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https://www.readbyqxmd.com/read/29149689/an-extended-sequence-specificity-for-uv-induced-dna-damage
#1
Long H Chung, Vincent Murray
The sequence specificity of UV-induced DNA damage was determined with a higher precision and accuracy than previously reported. UV light induces two major damage adducts: cyclobutane pyrimidine dimers (CPDs) and pyrimidine(6-4)pyrimidone photoproducts (6-4PPs). Employing capillary electrophoresis with laser-induced fluorescence and taking advantages of the distinct properties of the CPDs and 6-4PPs, we studied the sequence specificity of UV-induced DNA damage in a purified DNA sequence using two approaches: end-labelling and a polymerase stop/linear amplification assay...
November 2, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29149687/pyrrolidinyl-pna-polypyrrole-silver-nanofoam-electrode-as-a-novel-label-free-electrochemical-mirna-21-biosensor
#2
Tawatchai Kangkamano, Apon Numnuam, Warakorn Limbut, Proespichaya Kanatharana, Tirayut Vilaivan, Panote Thavarungkul
A label-free electrochemical miRNA biosensor was developed based on a pyrrolidinyl peptide nucleic acid (acpcPNA)/polypyrrole (PPy)/silver nanofoam (AgNF) modified electrode. The AgNF was electrodeposited as redox indicator on a gold electrode, which was then functionalized with an electropolymerized layer of PPy, a conducting polymer, to immobilize the PNA probes. The fabrication process was investigated by electrochemical impedance spectroscopy. The biosensor was used to detect miRNA-21, a biomarker abnormally expressed in most cancers...
November 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29149599/autophagosomal-content-profiling-reveals-an-lc3c-dependent-piecemeal-mitophagy-pathway
#3
François Le Guerroué, Franziska Eck, Jennifer Jung, Tatjana Starzetz, Michel Mittelbronn, Manuel Kaulich, Christian Behrends
Autophagy allows the degradation of cytosolic endogenous and exogenous material in the lysosome. Substrates are engulfed by double-membrane vesicles, coined autophagosomes, which subsequently fuse with lysosomes. Depending on the involvement of specific receptor proteins, autophagy occurs in a selective or nonselective manner. While this process is well understood at the level of bulky cargo such as mitochondria and bacteria, we know very little about individual proteins and protein complexes that are engulfed and degraded by autophagy...
November 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29149484/comparative-analysis-of-proteomes-between-diabetic-and-normal-human-sperm-insights-into-the-effects-of-diabetes-on-male-reproduction-based-on-the-regulation-of-mitochondria-related-proteins
#4
Tian An, Yue-Fen Wang, Jia-Xian Liu, Yan-Yun Pan, Yu-Fei Liu, Zhong-Chen He, Fang-Fang Mo, Jun Li, Li-Hua Kang, Yu-Jie Gu, Bo-Han Lv, Si-Huan Gao, Guang-Jian Jiang
This study sought to identify sources of the reduced fertility of men with type 2 diabetes mellitus. Significant reductions in semen volume, sperm concentration, and total sperm count were observed in diabetic individuals, while transmission electron microscopy revealed that the structure of mitochondria in the tail of sperm from diabetic patients was damaged. Proteins potentially associated with these sperm defects were identified using proteomics. Isobaric tagging for relative and absolute quantitation labeling and high-performance liquid chromatography-tandem mass spectrometry allowed us to identify 357 proteins significantly differentially expressed in diabetic versus control semen (>1...
November 17, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29149442/assessment-of-the-relative-contribution-of-volume-and-concentration-changes-in-yttrium-90-labelled-resin-microspheres-on-ionization-chamber-measurements
#5
Nicholas Forwood, Kathy P Willowson, Michael Tapner, Dale L Bailey
No abstract text is available yet for this article.
November 17, 2017: Australasian Physical & Engineering Sciences in Medicine
https://www.readbyqxmd.com/read/29149247/quantitative-proteomic-profiling-of-bovine-follicular-fluid-during-follicle-development
#6
Rodrigo de Andrade Ferrazza, Elizabeth Moreira Dos Santos Schmidt, Monika Mihm Carmichael, Fabiana Ferreira de Souza, Richard Burchmore, Roberto Sartori, Peter David Eckersall, João Carlos Pinheiro Ferreira
Bovine follicular fluid (FF) constitutes the microenvironment of follicles and includes various biologically active proteins. We performed a study involving 18 healthy non-lactating Holstein cows to determine the protein expression profile of FF at key-stages of follicular development. Follicles were individually aspirated in vivo at pre-deviation (F1∼7.0mm); deviation (F1∼8.5mm); post-deviation (F1∼12.0mm); and pre-ovulatory stages of follicle development, which were confirmed by measurement of follicular estradiol and progesterone concentrations...
November 15, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29149239/label-free-in-vivo-imaging-of-corneal-lymphatic-vessels-using-microscopic-optical-coherence-tomography
#7
Jens Horstmann, Hinnerk Schulz-Hildebrandt, Felix Bock, Sebastian Siebelmann, Eva Lankenau, Gereon Hüttmann, Philipp Steven, Claus Cursiefen
Purpose: Corneal neovascularization, in particular lymphangiogenesis, is a limiting factor in corneal transplant survival. Novel treatment approaches focus on (selective) inhibition and regression of lymphatic vessels. Imaging clinically invisible corneal lymphatic vessels is a prerequisite for these strategies. Using a murine model, this study investigates whether corneal lymphatic vessels can be imaged using microscopic optical coherence tomography (mOCT). Methods: Corneal neovascularization was induced by intrastromal placement of 11...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29149206/automatic-structural-scene-digitalization
#8
Rui Tang, Yuhan Wang, Darren Cosker, Wenbin Li
In this paper, we present an automatic system for the analysis and labeling of structural scenes, floor plan drawings in Computer-aided Design (CAD) format. The proposed system applies a fusion strategy to detect and recognize various components of CAD floor plans, such as walls, doors, windows and other ambiguous assets. Technically, a general rule-based filter parsing method is fist adopted to extract effective information from the original floor plan. Then, an image-processing based recovery method is employed to correct information extracted in the first step...
2017: PloS One
https://www.readbyqxmd.com/read/29149148/current-impact-and-application-of-abuse-deterrent-opioid-formulations-in-clinical-practice
#9
Ya-Han Lee, Daniel L Brown, Hsiang-Yin Chen
BACKGROUND: Abuse-deterrent formulations (ADFs) represent one novel strategy for curbing the potential of opioid abuse. OBJECTIVE: We aim to compare and contrast the characteristics and applications of current abuse-deterrent opioid products in clinical practice. METHODS: Literature searches were conducted in databases (Pubmed Medline, International Pharmaceutical Abstracts, Google Scholar) and official reports. Relevant data were screened and organized into: 1) epidemiology of opioid abuse, 2) mitigation strategies for reducing opioid abuse, 3) development of ADFs, and 4) clinical experience with these formulations...
November 2017: Pain Physician
https://www.readbyqxmd.com/read/29149147/hypothalamic-pituitary-adrenocortical-axis-suppression-following-a-single-epidural-injection-of-methylprednisolone-acetate
#10
Aleem Jameel Abdul, Babita Ghai, Dipika Bansal, Naresh Sachdeva, Anil Bhansali, Saravdeep Singh Dhatt
BACKGROUND: Epidural injections (EIs) are the most commonly performed minimally invasive intervention in managing chronic low back pain (CLBP). There is inconsistency in data to accurately predict the degree of hypothalamic-piuitary-adrenal (HPA) axis suppression in patients receiving exogenous steroid therapy, especially in the form of epidural steroid injections (ESIs). OBJECTIVE: We aim to quantify the degree and duration of HPA axis suppression after a single ESI of 80 mg methyl prednisolone acetate in patients with CLBP...
November 2017: Pain Physician
https://www.readbyqxmd.com/read/29148818/dissociation-constants-of-cytochrome-p450-2c9-cytochrome-p450-reductase-complexes-in-a-lipid-bilayer-membrane-depend-on-nadph-a-single-protein-tracking-study
#11
Carlo Barnaba, Evan Taylor, James A Brozik
Cytochrome P450-Reductase (CPR) is a versatile NADPH-dependent electron donor located in the cytoplasmic side of the endoplasmic reticulum. It is an electron transferase that is able to deliver electrons to a variety of membrane-bound oxidative partners, including the drug-metabolizing enzymes of the cytochrome P450s (P450). CPR is also stoichiometrically limited compared to its oxidative counterparts and hypotheses have arisen about possible models that can overcome the stochiometric imbalance, including quaternary organization of P450 and diffusion-limited models...
November 17, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29148785/rapid-quantitative-measurements-of-paramagnetic-relaxation-enhancements-in-cu-ii-tagged-proteins-by-proton-detected-solid-state-nmr-spectroscopy
#12
Dwaipayan Mukhopadhyay, Philippe S Nadaud, Matthew D Shannon, Christopher P Jaroniec
We demonstrate rapid quantitative measurements of site-resolved paramagnetic relaxation enhancements (PREs), which are a source of valuable structural restraints corresponding to electron-nucleus distances in the ~10-20 Å regime, in solid-state nuclear magnetic resonance (NMR) spectra of proteins containing covalent Cu(2+)-binding tags. Specifically, using protein GB1 K28C-EDTA-Cu(2+) mutant as a model, we show the determination of backbone amide (15)N longitudinal and (1)H transverse PREs within a few hours of experiment time based on proton-detected 2D or 3D correlation spectra recorded with magic-angle spinning frequencies ≥ ~60 kHz for samples containing ~10-50 nanomoles of (2)H,(13)C,(15)N-labeled protein back-exchanged in H2O...
November 17, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29148726/a-novel-approach-for-99mtc-labeling-of-red-blood-cells-evaluation-of-99mtc-4saboroxime-as-a-blood-pool-imaging-agent
#13
Min Liu, Zuoquan Zhao, Wei Fang, Shuang Liu
Angiography with radiolabeled red blood cells (RBCs) plays an important role in diagnosis and prognosis in vascular diseases. Both in vitro and in vivo methods have been developed for 99mTc-labeling of RBCs. However, these methods are complicated and lack of reproducibility. Therefore, it is highly desirable to develop an alternative method for routine 99mTc-labeling of RBCs. In this report, we present a novel approach for 99mTc-labeling of RBCs. We prepared a new 99mTc(III) radiotracer [99mTcCl(CDO)(CDOH)2B-4AS] (99mTc-4ASboroxime: 4AS-B(OH)2 = 4-aminosulfonylphenyl)boronic acid, and CDOH2 = cyclohexanedione dioxime) in >95% radiochemical purity...
November 17, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29148722/acoustic-wave-driven-functionalized-particles-for-aptamer-based-target-biomolecule-separation
#14
Raheel Ahmad, Ghulam Destgeer, Muhammad Afzal, Jinsoo Park, Husnain Ahmed, Jin Ho Jung, Kwangseok Park, Tae-Sung Yoon, Hyung Jin Sung
We developed a hybrid microfluidic device that utilized acoustic waves to drive functionalized microparticles inside a continuous flow microchannel and to separate particle-conjugated target proteins from a complex fluid. The acoustofluidic device comprised of an interdigitated transducer (IDT) that produced high-frequency surface acoustic waves (SAW) in a polydimethylsiloxane (PDMS) microfluidic channel. The SAW interacted with the sample fluid flow inside the microchannel and deflected particles from their original streamlines to achieve separation...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148720/a-dual-signal-readout-nanosphere-for-rapid-point-of-care-detection-of-ebola-virus-glycoprotein
#15
Jiao Hu, Yong-Zhong Jiang, Ling-Ling Wu, Zhen Wu, Yuhai Bi, Gary Wong, Xiang-Guo Qiu, Jian-Jun Chen, Dai-Wen Pang, Zhi-Ling Zhang
Rapid detection of highly contagious pathogens is the key to increasing the probability of survival and reducing infection rates. We developed a sensitive and quantitative lateral flow assay for detection of EBOV glycoprotein with a novel multifunctional nanosphere (RNs@Au) as a reporter. Each RNs@Au contains hundreds of quantum dots and dozens of Au nanoparticles and can achieve enhanced dual-signal readout (fluorescence signal for quantitative detection and colorimetric signal for visual detection). Antibody (Ab) and streptavidin (SA) were simultaneously modified onto the RNs@Au to label the target as well as to act as the signal enhancer...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148719/effect-of-antigen-retrieval-methods-on-non-specific-binding-of-anti-body-metal-nanoparticle-conjugates-on-ffpe-tissue
#16
Yuying Zhang, Xinping Wang, Sven Perner, Ágnes Bánkfalvi, Sebastian Schlücker
Immunohistochemical analysis of FFPE tissues provides important diagnostic and prognostic information in pathology. Metal nanoparticles (NPs) and in particular surface-enhanced Raman scattering (SERS) nanotags as a new class of labelling reagents are promising to be used for multiplexed protein profiling on tissue sections. However, non-specific binding of NPs onto the tissue specimens greatly hampers their clinical applications. In this study, we found that the antigen retrieval method strongly influences the extent of non-specific binding of the antibody-SERS NP conjugates to the tissue...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29148702/influence-of-particle-geometry-on-gastrointestinal-transit-and-absorption-following-oral-administration
#17
Dong Li, Jie Zhuang, Haisheng He, Sifan Jiang, Amrita Banerjee, Yi Lu, Wei Wu, Samir Mitragotri, Li Gan, Jianping Qi
Geometry has been considered as one of the important parameters in nanoparticle design since it affects cellular uptake, transport across the physiological barriers and in vivo distribution. However, only a few studies have been conducted to elucidate the influence of nanoparticle geometry in their in vivo fate after oral administration. This paper discloses the effect of nanoparticle shape on transport and absorption in gastrointestinal tract (GIT). Nanorods and nanospheres were prepared, and labeled using fluorescence resonance energy transfer (FRET) molecules to track the in vivo fate of intact nanoparticles accurately...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29148698/chelator-free-conjugation-of-99mtc-and-gd3-to-pegylated-nanographene-oxide-for-dual-modality-spect-mr-imaging-of-lymph-nodes
#18
Tianye Cao, Xiaobao Zhou, Yingying Zheng, Yuyun Sun, Jian Zhang, Wei Chen, Jianping Zhang, Zhiguo Zhou, Shi-Ping Yang, Yingjian Zhang, Hong Yang, Ming-Wei Wang
PEGylated ultra-small nanographene oxide (usNGO-PEG) has exhibited great potential in nanotheranostics due to its newly-discovered physicochemical properties derived from the rich functional groups and  bonds. Herein, we developed a general, simple and kit-like preparation approach for 99mTcI- and Gd-anchored NGO-PEG using a chelator-free strategy. In this strategy, [99mTcI(CO)3(OH2)3]+ (abbreviated to 99mTcI) and GdCl3 were mixed with usNGO-PEG to yield 99mTc- and Gd-usNGO-PEG via the synergistic coordination of N and O atoms from NGO and PEG with 99mTcI and Gd3+ without additional exogenous chelators...
November 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29148535/the-role-of-apc-in-wnt-pathway-activation-in-serrated-neoplasia
#19
Jennifer Borowsky, Troy Dumenil, Mark Bettington, Sally-Ann Pearson, Catherine Bond, Lochlan Fennell, Cheng Liu, Diane McKeone, Christophe Rosty, Ian Brown, Neal Walker, Barbara Leggett, Vicki Whitehall
Conventional adenomas are initiated by APC gene mutation that activates the WNT signal. Serrated neoplasia is commonly initiated by BRAF or KRAS mutation. WNT pathway activation may also occur, however, to what extent this is owing to APC mutation is unknown. We examined aberrant nuclear β-catenin immunolocalization as a surrogate for WNT pathway activation and analyzed the entire APC gene coding sequence in serrated and conventional pathway polyps and cancers. WNT pathway activation was a common event in conventional pathway lesions with aberrant nuclear immunolocalization of β-catenin and truncating APC mutations in 90% and 89% of conventional adenomas and 82% and 70% of BRAF wild-type cancers, respectively...
November 17, 2017: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29148291/effectiveness-and-safety-of-anti-ischemic-trimetazidine-in-patients-with-stable-angina-pectoris-and-type-2-diabetes
#20
Zsófia Meiszterics, Attila Kónyi, Gábor Hild, Zsolt Sárszegi, Balázs Gaszner
Aim & methods: This 6-month prospective, observational, noninterventional, open-label clinical study assessed the effectiveness/safety of trimetazidine in 737 patients with stable angina pectoris and Type 2 diabetes mellitus (OGYI/51534-1/2014). RESULTS: Trimetazidine-based therapy was effective in stable coronary artery disease, with significant improvements from baseline (p < 0.05) in: number of angina attacks/week (2.9 ± 2.4 vs 1.1 ± 1.6), angina severity (Canadian Cardiovascular Society Classification 1...
November 17, 2017: Journal of Comparative Effectiveness Research
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