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HSP27 extracellular

Kirsten F Smit, Daniel Brevoord, Stefan De Hert, Bas A de Mol, Raphaela P Kerindongo, Susan van Dieren, Wolfgang S Schlack, Markus W Hollmann, Nina C Weber, Benedikt Preckel
BACKGROUND: The noble gas helium induces pre- and postconditioning in animals and humans. Volatile anesthetics induce cardioprotection in humans undergoing coronary artery bypass graft (CABG) surgery. We hypothesized that helium induces pre- and postconditioning in CABG-patients, affecting signaling molecules protein kinase C-epsilon (PKC-ε), p38 mitogen activated protein kinase (p38 MAPK), extracellular signal-regulated kinase 1/2 (ERK-1/2) and heat shock protein 27 (HSP-27) within cardiac tissue, and reducing postoperative troponin levels...
October 14, 2016: Journal of Translational Medicine
Gwangchul Lee, Bora Kim, Youngjong Ko, Mineon Park, Donghwi Kim, Kang Hyeon Ryu, Yong Cheol Jun, Hong Moon Sohn, Wonbong Lim
OBJECTIVE: This study was designed to investigate the effect of 635-nm irradiation from a light emitting diode (LED) on osteoclastogenesis in receptor activator of nuclear factor kappa-B (NF-κB) ligand (RANKL)-stimulated mouse bone marrow-derived macrophages (BMMs). We determined whether 635-nm irradiation modulated the RANKL-induced osteoclastic signaling pathway in heat shock protein-27 (HSP27)-silenced cells and analyzed the functional cross talk between these factors in osteoclastic differentiation and activation...
September 14, 2016: Photomedicine and Laser Surgery
Zarah Batulan, Vivek Krishna Pulakazhi Venu, Yumei Li, Geremy Koumbadinga, Daiana Gisela Alvarez-Olmedo, Chunhua Shi, Edward R O'Brien
Heat shock protein 27 (HSP27) is traditionally viewed as an intracellular chaperone protein with anti-apoptotic properties. However, recent data indicate that a number of heat shock proteins, including HSP27, are also found in the extracellular space where they may signal via membrane receptors to alter gene transcription and cellular function. Therefore, there is increasing interest in better understanding how HSP27 is released from cells, its levels and composition in the extracellular space, and the cognate cell membrane receptors involved in effecting cell signaling...
2016: Frontiers in Immunology
Joanna Homa, Weronika Ortmann, Elzbieta Kolaczkowska
Formation of extracellular traps (ETs) capturing and immobilizing pathogens is now a well-established defense mechanism added to the repertoire of vertebrate phagocytes. These ETs are composed of extracellular DNA (extDNA), histones and antimicrobial proteins. Formation of mouse and human ETs depends on enzymes (i) facilitating decondensation of chromatin by citrullination of histones, and (ii) serine proteases degrading histones. In invertebrates, initial reports revealed existence of ETs composed of extDNA and histones, and here we document for the first time that also coelomocytes, immunocompetent cells of an earthworm Eisenia andrei, cast ETs which successfully trap bacteria in a reactive oxygen species (ROS)-dependent and -independent manner...
2016: PloS One
Matthias B Stope, Luise Wiegank, Martin Weiss, Karoline Diesing, Dominique Koensgen, Martin Burchardt, Marek Zygmunt, Alexander Mustea
BACKGROUND: Heat-shock protein HSPB1 (alternative name HSP27) plays a pivotal role in cell survival pathways, apoptosis, metastasis and has been frequently linked to treatment resistance in ovarian cancer (OC) and other malignancies. Characteristic HSPB1 induction in different solid tumors is often caused by cytotoxic agents. MATERIALS AND METHODS: An in vitro OC cell model system was established to characterize resistance mechanisms during chemotherapy. Human OC cell lines OVCAR-3, SK-OV-3 and TOV-21G were treated with paclitaxel or carboplatin...
July 2016: Anticancer Research
Jorge E Toblli, Gabriel Cao, Carlos Rivas, Jorge F Giani, Fernando P Dominici
BACKGROUND: According to recent clinical trial data, correction of iron deficiency with intravenous (i.v.) iron has favorable outcomes on cardiac function. We evaluated whether i.v. iron treatment of anemic rats has favorable effect on the left ventricular (LV) performance and remodeling and the role of oxidative/nitrosative stress and inflammation in the process. METHODS: After weaning, Sprague-Dawley rats were fed low iron diet for 16weeks, after which the treatment group received five weekly doses of i...
June 1, 2016: International Journal of Cardiology
Vincent Andermark, Katrin Göke, Malte Kokoschka, Mohamed A Abu El Maaty, Ching Tung Lum, Taotao Zou, Raymond Wai-Yin Sun, Elisabet Aguiló, Luciano Oehninger, Laura Rodríguez, Heike Bunjes, Stefan Wölfl, Chi-Ming Che, Ingo Ott
Gold alkynyl complexes with phosphane ligands of the type (alkynyl)Au(I)(phosphane) represent a group of bioorganometallics, which has only recently been evaluated biologically in more detail. Structure-activity-relationship studies regarding the residues of the phosphane ligand (P(Ph)3, P(2-furyl)3, P(DAPTA)3, P(PTA)3, P(Et)3, P(Me)3) of complexes with an 4-ethynylanisole alkyne ligand revealed no strong differences concerning cytotoxicity. However, a relevant preference for the heteroatom free alkyl/aryl residues concerning inhibition of the target enzyme thioredoxin reductase was evident...
July 2016: Journal of Inorganic Biochemistry
Alice A Han, Holly N Currie, Matthew S Loos, Julie A Vrana, Emily B Fabyanic, Maren S Prediger, Jonathan W Boyd
Molecular mechanisms of wound healing have been extensively characterized, providing a better understanding of the processes involved in wound repair and offering advances in treatment methods. Both spatial and temporal investigations of injury biomarkers have helped to pinpoint significant time points and locations during the recovery process, which may be vital in managing the injury and making the appropriate diagnosis. This study addresses spatial and temporal differences of phosphoproteins found in skeletal muscle tissue following a traumatic femur fracture, which were further compared to co-localized cytokine responses...
March 2016: Cytokine
Kyle K Biggar, Cheng-Wei Wu, Shannon N Tessier, Jing Zhang, Fabien Pifferi, Martine Perret, Kenneth B Storey
Very few selected species of primates are known to be capable of entering torpor. This exciting discovery means that the ability to enter a natural state of dormancy is an ancestral trait among primates and, in phylogenetic terms, is very close to the human lineage. To explore the regulatory mechanisms that underlie primate torpor, we analyzed signal transduction cascades to discover those involved in coordinating tissue responses during torpor. The responses of mitogen-activated protein kinase (MAPK) family members to primate torpor were compared in six organs of control (aroused) versus torpid gray mouse lemurs, Microcebus murinus...
April 2015: Genomics, Proteomics & Bioinformatics
Roland Scholz, Koshi Imami, Nichollas E Scott, William S Trimble, Leonard J Foster, B Brett Finlay
Enteropathogenic Escherichia coli (EPEC) uses a type III secretion system (T3SS) to directly translocate effector proteins into host cells where they play a pivotal role in subverting host cell signaling needed for disease. However, our knowledge of how EPEC affects host protein phosphorylation is limited to a few individual protein studies. We employed a quantitative proteomics approach to globally map alterations in the host phosphoproteome during EPEC infection. By characterizing host phosphorylation events at various time points throughout infection, we examined how EPEC dynamically impacts the host phosphoproteome over time...
July 2015: Molecular & Cellular Proteomics: MCP
Ahmed F El-Yazbi, Khaled S Abd-Elrahman, Alejandro Moreno-Dominguez
Defective protein kinase C (PKC) signaling has been suggested to contribute to abnormal vascular contraction in disease conditions including hypertension and diabetes. Our previous work on agonist and pressure-induced cerebral vasoconstriction implicated PKC as a major contributor to force production in a myosin light chain (LC20) phosphorylation-independent manner. Here, we used phorbol dibutyrate to selectively induce a PKC-dependent constriction in rat middle cerebral arteries and delineate the relative contribution of different contractile mechanisms involved...
June 15, 2015: Biochemical Pharmacology
Isabel Fernandez, Abel Martin-Garrido, Dennis W Zhou, Roza E Clempus, Bonnie Seidel-Rogol, Alejandra Valdivia, Bernard Lassègue, Andrés J García, Kathy K Griendling, Alejandra San Martin
OBJECTIVE: Focal adhesions (FAs) link the cytoskeleton to the extracellular matrix and as such play important roles in growth, migration, and contractile properties of vascular smooth muscle cells. Recently, it has been shown that downregulation of Nox4, a transforming growth factor (TGF) β-inducible, hydrogen peroxide (H2O2)-producing enzyme, affects the number of FAs. However, the effectors downstream of Nox4 that mediate FA regulation are unknown. The FA resident protein H2O2-inducible clone (Hic)-5 is H2O2 and TGFβ inducible, and a binding partner of the heat shock protein (Hsp) 27...
May 2015: Arteriosclerosis, Thrombosis, and Vascular Biology
Tushar B Deb, Annie H Zuo, Robert J Barndt, Surojeet Sengupta, Radmila Jankovic, Michael D Johnson
The gene for Pregnancy Up-regulated Non-ubiquitous Calmodulin Kinase (Pnck), a novel calmodulin kinase, is expressed in roughly one-third of human breast tumors, but not in adjoining normal tissues. Pnck alters EGFR stability and function, prompting this study to determine if Pnck expression has implications for HER-2 function and HER-2-directed therapy. The frequency of Pnck expression in HER-2-amplified breast cancer was examined by immunohistochemistry, and the impact of Pnck expression in the presence of HER-2 amplification on cancer cell proliferation, clonogenicity, cell-cycle progression, and Trastuzumab sensitivity was examined in vitro by transfection of cells with Pnck...
April 2015: Breast Cancer Research and Treatment
Hiroyuki Nishi, Noriko Maeda, Shunsuke Izumi, Sayomi Higa-Nakamine, Seikichi Toku, Manabu Kakinohana, Kazuhiro Sugahara, Hideyuki Yamamoto
In previous studies, we found that stimulation of Toll-like receptor 5 (TLR5) by flagellin induced the activation of mitogen-activated protein kinase (MAPK)-activated protein kinase-2 (MAPKAPK-2) through activation of the p38 MAPK pathway in cultured alveolar epithelial A549 cells. Our studies strongly suggested that MAPKAPK-2 phosphorylated epidermal growth factor receptor (EGFR) at Ser1047. It has been reported that phosphorylation of Ser1047 after treatment with tumor necrosis factor α (TNFα) induced the internalization of EGFR...
February 5, 2015: European Journal of Pharmacology
Jian-ling Xia, Li-qun Wang, Li-li Wu, Qiao-bing Huang
Doxycycline hyclate (DOX-h) attenuates inflammatory conditions independent of its antimicrobial effect. This study aimed to observe the effects of DOX-h on lipopolysaccharide (LPS)-induced endothelial barrier dysfunction. The endothelial monolayer permeability of human umbilical vein endothelial cells (HUVECs) was monitored by transendothelial electrical resistance (TEER). The phosphorylation of mitogen-activated protein kinases (MAPKs) and the arrangement of F-actin were detected. The results showed that both pretreatment and simultaneous treatment with DOX-h markedly attenuated the LPS-induced reduction in TEER and the disorganization of F-actin on HUVECs in a dose- and time-dependent manner...
2014: Biological & Pharmaceutical Bulletin
Hai-Feng Chen, Shu-Juan Liu, Gang Chen
In the present study, the effect of the heat shock protein 27 (HSP27) signaling pathway on the proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs) induced by high glucose (HG) was investigated. HUVEC proliferation in the indicated conditions was measured by the alamarBlue® assay. Apoptosis in HUVECs cultured with HG was analyzed by an Annexin V‑fluorescein isothiocyanate/propidium iodide apoptosis detection kit. HSP27 activity was evaluated by western blotting with specific phospho‑HSP27 antibody...
February 2015: Molecular Medicine Reports
Edna Suarez, Farhatullah Syed, Teresa Alonso-Rasgado, Ardeshir Bayat
Among raised dermal scar types, keloid (KS) and hypertrophic scars (HS) are considered to present clinical similarities, but there are no known specific biomarkers that allow both scar types to be easily distinguished. Development and progression of raised dermal scars comprises the activation of several molecular pathways and cell defence mechanisms leading to elevated extracellular matrix component synthesis, delayed apoptosis, altered migration and differentiation. Therefore, the aim here was to identify biomarkers that may differentiate between KS and HS compared to normal skin (NS)...
March 2015: Archives of Dermatological Research
Marcellino Monda, Giovanni Messina, Ilaria Scognamiglio, Angela Lombardi, Giuseppe A Martin, Pasquale Sperlongano, Marina Porcelli, Michele Caraglia, Paola Stiuso
The present study was designed to evaluate the effects of diet lifestyle on extending lifespan and reducing liver cancer risk. Young overweight men (n = 20), without metabolic syndrome, were placed in a 3-week residential program on a low-fat diet and moderate aerobic exercise. In each subject, pre- and postintervention fasting blood were collected for evaluating levels of serum lipids, and oxidative stress markers. Using subject sera and cardiomyocyte (H9C2) culture systems, we measured heat shock protein 27 and 90 expression, lipid accumulation, and oxidative stress marker levels...
2014: Oxidative Medicine and Cellular Longevity
T Usui, R Nijima, T Sakatsume, K Otani, S Kameshima, M Okada, H Yamawaki
AIM: Eukaryotic elongation factor 2 kinase (eEF2K), also known as calmodulin (CaM)-dependent protein kinase (CaMK) III, is a unique member of CaMK family protein. We have recently found that expression of eEF2K protein increased in mesenteric artery from spontaneously hypertensive rats. As pathogenesis of hypertension is in part regulated by vascular structural remodelling via proliferation and migration of vascular smooth muscle cells (SMCs), we tested the hypothesis that eEF2K controls SMCs proliferation and migration...
February 2015: Acta Physiologica
Edna Suarez, Farhatullah Syed, Teresa A Rasgado, Alan Walmsley, Parthasarathi Mandal, Ardeshir Bayat
Skin tension may influence keloid scar behavior, development, and spreading, e.g., butterfly-shaped keloid disease in the sternum. Here, we developed a three-dimensional (3D) in vitro model to mimic in vivo tension and evaluate keloid fibroblast (KF) behavior and extracellular matrix synthesis under tension. In vivo skin tension measured in volunteers (n = 4) using 3D image photogrammetry enabled prediction of actual force (35 mN). A novel cell force monitor applied tension in a fibroblast-populated 3D collagen lattice replicating the in vivo force...
September 2014: Wound Repair and Regeneration
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