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loop-mediated isothermal amplification

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https://www.readbyqxmd.com/read/28427352/point-of-sampling-detection-of-zika-virus-within-a-multiplexed-kit-capable-of-detecting-dengue-and-chikungunya
#1
Ozlem Yaren, Barry W Alto, Priyanka V Gangodkar, Shatakshi R Ranade, Kunal N Patil, Kevin M Bradley, Zunyi Yang, Nikhil Phadke, Steven A Benner
BACKGROUND: Zika, dengue, and chikungunya are three mosquito-borne viruses having overlapping transmission vectors. They cause diseases having similar symptoms in human patients, but requiring different immediate management steps. Therefore, rapid (< one hour) discrimination of these three viruses in patient samples and trapped mosquitoes is needed. The need for speed precludes any assay that requires complex up-front sample preparation, such as extraction of nucleic acids from the sample...
April 20, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28427163/the-application-of-is6110-baced-loop-mediated-isothermal-amplification-lamp-in-the-early-diagnosis-of-tuberculous-meningitis
#2
Wen-Wen Sun, Qin Sun, Li-Ping Yan, Qing Zhang
Here, we evaluated the potential activity of rapid Mycobacterium tuberculosis detection with loop-mediated isothermal amplification (LAMP), for the early diagnosis of tuberculous meningitis (TBM). Patients with suspected TBM from January 2014 to December 2015 were reviewed retrospectively. The cerebrospinalfluid(CSF) was collected. Acid-fast bacillus (AFB) staining, MGIT 960 culture, real-time fluorescent quantitative polymerase chain reaction (RTFQ PCR) and LAMP were performed. A total of 200 patients were included in the study...
February 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28424530/molecular-diagnostic-assays-based-on-cpn60-ut-sequences-reveal-the-geographic-distribution-of-subgroup-16srxiii-a-i-i-phytoplasma-in-mexico
#3
Edel Pérez-López, Douglas Rodríguez-Martínez, Chrystel Y Olivier, Mauricio Luna-Rodríguez, Tim J Dumonceaux
Geographically diverse samples from strawberry exhibiting symptoms of Strawberry Green Petal (SbGP), periwinkle plants with virescence, and blackberry, blueberry, and raspberry plants displaying yellowing and inedible fruits, were assayed for the presence of phytoplasma DNA. PCR targeting the 16S rRNA-encoding gene and chaperonin-60 (cpn60) showed that the plants were infected with phytoplasma subgroup16SrXIII-(A/I)I (SbGP/MPV). To examine the geographic distribution of this pathogen in Mexico, we designed an array of cpn60-targeted molecular diagnostic assays for SbGP/MPV phytoplasma...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28405200/corrigendum-loop-mediated-isothermal-amplification-method-for-the-rapid-detection-of-ralstonia-solanacearum-phylotype-i-mulberry-strains-in-china
#4
Wen Huang, Hao Zhang, Jingsheng Xu, Shuai Wang, Xiangjiu Kong, Wei Ding, Jin Xu, Jie Feng
[This corrects the article on p. 76 in vol. 8, PMID: 28197157.].
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28392412/detection-on-integron-carried-gene-cassettes-from-pathogens-by-loop-mediated-isothermal-amplification-assays
#5
Guangchao Yu, Congxiu Ye, Qiang Fu, Juzhen Liu, Xiaoyi Fan, Yunzu Huang, Shishui Zhou
In this study, a number of frequently detected gene cassettes from bacterial integrons have been detected and characterized by rapid and simple loop-mediated isothermal amplification (LAMP) assays. Six gene cassettes commonly found in class 1 integrons were studied, including dfrA12, dfrA17, aadA2, aadA5, orfF, and blaVIM2. Primers design, sensitivity, specificity, optimization of each LAMP assay, as well as application of the developed 6 individual LAMP assays on a large scale of bacteria, had been conducted...
April 6, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28381965/development-and-evaluation-of-loop-mediated-isothermal-amplification-assay-for-rapid-detection-of-tylenchulus-semipenetrans-using-dna-extracted-from-soil
#6
Zhi-Qiang Song, Ju-E Cheng, Fei-Xue Cheng, De-Yong Zhang, Yong Liu
Tylenchulus semipenetrans is an important and widespread plant-parasitic nematode of citrus worldwide and can cause citrus slow decline disease leading to significant reduction in tree growth and yield. Rapid and accurate detection of T. semipenetrans in soil is important for the disease forecasting and management. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed to detect T. semipenetrans using DNA extracted from soil. A set of five primers was designed from the internal transcribed spacer region (ITS1) of rDNA, and was highly specific to T...
April 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28377900/loop-mediated-isothermal-amplification-lamp-for-embryo-sex-determination-in-pregnant-women-at-eight-weeks-of-pregnancy
#7
Mohammad Amin Almasi, Galavizh Almasi
BACKGROUND: In human, SRY (sex-determining region of the Y chromosome) is the major gene for the testis-determining factor which is found in normal XY males and in the rare XX males, and it is absent in normal XX females and many XY females. There are several methods which can indicate a male genotype by the presence of the amplified product of SRY gene. The aim of this study was to identify the SRY gene for embryo sex determination in human during pregnancy using loop mediated isothermal amplification (LAMP) method...
January 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28372729/development-of-a-thermostabilised-triplex-lamp-assay-with-dry-reagent-four-target-lateral-flow-dipstick-for-detection-of-entamoeba-histolytica-and-non-pathogenic-entamoeba-spp
#8
Phiaw Chong Foo, Yean Yean Chan, Maizan Mohamed, Weng Kin Wong, A B Nurul Najian, Boon Huat Lim
This study highlighted the development of a four target nitrocellulose-based nucleic acid lateral flow immunoassay biosensor in a dry-reagent strip format for interpretation of double-labelled double-stranded amplicons from thermostabilised triplex loop-mediated isothermal amplification assay. The DNA biosensor contained two test lines which captured biotin and texas red labelled amplicons; a LAMP internal amplification control line that captured digoxigenin labelled amplicon; and a chromatography control line that validated the functionality of the conjugated gold nanoparticles and membrane...
May 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28368036/rapid-simple-and-direct-detection-of-meloidogyne-hapla-from-infected-root-galls-using-loop-mediated-isothermal-amplification-combined-with-fta-technology
#9
Huan Peng, Haibo Long, Wenkun Huang, Jing Liu, Jiangkuan Cui, Lingan Kong, Xianqi Hu, Jianfeng Gu, Deliang Peng
The northern root-knot nematode (Meloidogyne hapla) is a damaging nematode that has caused serious economic losses worldwide. In the present study, a sensitive, simple and rapid method was developed for detection of M. hapla in infested plant roots by combining a Flinders Technology Associates (FTA) card with loop-mediated isothermal amplification (LAMP). The specific primers of LAMP were designed based on the distinction of internal transcribed spacer (ITS) sequences between M. hapla and other Meloidogyne spp...
April 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28359328/stem-loop-mediated-isothermal-amplification-test-comparative-analysis-with-classical-lamp-and-pcr-in-detection-of-entamoeba-histolytica-in-kenya
#10
Fridah Mwendwa, Cecilia K Mbae, Johnson Kinyua, Erastus Mulinge, Gitonga Nkanata Mburugu, Zablon K Njiru
BACKGROUND: Entamoeba histolytica, the causative agent for amoebiasis is a considerable burden to population in the developing countries where it accounts for over 50 million infections. The tools for detection of amoebiasis are inadequate and diagnosis relies on microscopy which means a significant percent of cases remain undiagnosed. Moreover, tests formats that can be rapidly applied in rural endemic areas are not available. METHODS: In this study, a loop-mediated isothermal test (LAMP) based on 18S small subunit ribosomal RNA gene was designed with extra reaction accelerating primers (stem primers) and compared with the published LAMP and PCR tests in detection of E...
March 31, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28356221/rapid-detection-of-all-known-ebolavirus-species-by-reverse-transcription-loop-mediated-isothermal-amplification-rt-lamp
#11
Olamide K Oloniniyi, Yohei Kurosaki, Hiroko Miyamoto, Ayato Takada, Jiro Yasuda
Ebola virus disease (EVD), a highly virulent infectious disease caused by ebolaviruses, has a fatality rate of 25-90%. Without a licensed chemotherapeutic agent or vaccine for the treatment and prevention of EVD, control of outbreaks requires accurate and rapid diagnosis of cases. In this study, five sets of six oligonucleotide primers targeting the nucleoprotein gene were designed for specific identification of each of the five ebolavirus species using reverse transcription-loop mediated isothermal amplification (RT-LAMP) assay...
March 26, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28352046/evaluation-of-sybr-green-i-based-visual-loop-mediated-isothermal-amplification-lamp-assay-for-genus-and-species-specific-diagnosis-of-malaria-in-p-vivax-and-p-falciparum-endemic-regions
#12
COMPARATIVE STUDY
Ruchi Singh, Dhirendra Pratap Singh, Deepali Savargaonkar, Om P Singh, Rajendra M Bhatt, Neena Valecha
BACKGROUND & OBJECTIVES: Loop-mediated isothermal amplification (LAMP) is an emerging nucleic acid based diag- nostic approach that is easily adaptable to the field settings with limited technical resources. This study was aimed to evaluate the LAMP assay for the detection and identification of Plasmodium falciparum and P. vivax infection in malaria suspected cases using genus and species-specific assay. METHODS: The 18S rRNA-based LAMP assay was evaluated for diagnosis of genus Plasmodium, and species- specific diagnosis of P...
January 2017: Journal of Vector Borne Diseases
https://www.readbyqxmd.com/read/28351721/a-rapid-simple-and-sensitive-loop-mediated-isothermal-amplification-method-to-detect-anaplasma-bovis-in-sheep-and-goats-samples
#13
Jinhong Wang, Yan Zhang, Yanyan Cui, Yaqun Yan, Xiaoxing Wang, Rongjun Wang, Fuchun Jian, Longxian Zhang, Changshen Ning
A loop-mediated isothermal amplification (LAMP) technique has been widely used in detecting the nucleic acid of various pathogenic bacteria. In this study, a set of four LAMP primers was designed to specifically test Anaplasma bovis. The LAMP assay was performed at 62°C for 60min in a water bath. The specificity was confirmed by amplifying A. bovis isolate, while no cross reaction was observed with other five pathogens (Anaplasma bovis, Anaplasma phagocytophilum, Theileria luwenshuni, Babesia motasi and Schistosoma japonicum)...
March 25, 2017: Parasitology International
https://www.readbyqxmd.com/read/28349967/development-of-a-multiplex-loop-mediated-isothermal-amplification-method-for-the-simultaneous-detection-of-salmonella-spp-and-vibrio-parahaemolyticus
#14
Ningwei Liu, Dayang Zou, Derong Dong, Zhan Yang, Da Ao, Wei Liu, Liuyu Huang
Rapid detection of food-borne pathogens is important in the food industry, to monitor and prevent the spread of these pathogens through contaminated food products. We therefore established a multiplex real-time loop-mediated isothermal amplification (LAMP) assay to simultaneously detect and distinguish Salmonella spp. and Vibrio parahaemolyticus DNA in a single reaction. Two target sequences, one specific for Salmonella and the other specific for Vibrio parahaemolyticus, were amplified by specific LAMP primers in the same reaction tube...
March 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28339040/detection-assay-for-hpv16-and-hpv18-by-loop%C3%A2-mediated-isothermal-amplification-with-lateral-flow-dipstick-tests
#15
Ratchanida Kumvongpin, Patcharee Jearanaikoon, Chotchana Wilailuckana, Nattaya Sae-Ung, Prinya Prasongdee, Sakda Daduang, Metee Wongsena, Patcharee Boonsiri, Wansika Kiatpathomchai, Sukumarn Sanersak Swangvaree, Alisa Sandee, Jureerut Daduang
Cervical cancer is the third highest cause of death in developing countries and most commonly results from high‑risk human papillomavirus (HR‑HPV) infection. Among HR‑HPV genotypes, HPV16 and HPV18 are the most prevalent in cervical cancers. Therefore, the present study aimed to develop a detection assay for HPV16 and HPV18 infection using loop‑mediated isothermal amplification (LAMP) with lateral flow dipstick (LFD) tests. This assay is a simplified, user‑friendly method for the visual detection of HPV genotypes...
March 23, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28338022/alizarin-red-s-for-online-pyrophosphate-detection-identified-by-a-rapid-screening-method
#16
Jens Fischbach, Qiuting Loh, Frank F Bier, Theam Soon Lim, Marcus Frohme, Jörn Glökler
We identified Alizarin Red S and other well known fluorescent dyes useful for the online detection of pyrophosphate in enzymatic assays, including the loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays. An iterative screening was used for a selected set of compounds to first secure enzyme compatibility, evaluate inorganic pyrophosphate sensitivity in the presence of manganese as quencher and optimize conditions for an online detection. Of the selected dyes, the inexpensive alizarin red S was found to selectively detect pyrophosphate under LAMP and PCR conditions and is superior with respect to its defined red-shifted spectrum, long shelf life and low toxicity...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28335829/comparison-of-nasopharyngeal-and-guttural-pouch-specimens-to-determine-the-optimal-sampling-site-to-detect-streptococcus-equi-subsp-equi-carriers-by-dna-amplification
#17
Ashley G Boyle, Darko Stefanovski, Shelley C Rankin
BACKGROUND: Streptococcus equi subsp equi (S. equi) is the cause of "equine strangles" which is a highly infectious upper respiratory disease. Detection of S. equi is influenced by site of specimen collection, method of sampling, and type of diagnostic test that is performed. We hypothesized i) that a loop-mediated isothermal amplification (LAMP) assay that targets the S. equi-specific eqbE gene would be more sensitive than a realtime PCR assay that targets the S. equi-specific seeI gene and ii) that LAMP of specimens obtained by guttural pouch lavage (GPL) would be more sensitive than LAMP of nasopharyngeal specimens to identify S...
March 23, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28335752/development-of-a-rapid-loop-mediated-isothermal-amplification-assay-for-diagnosis-and-assessment-of-cure-of-leishmania-infection
#18
Sandeep Verma, Ruchi Singh, Vanila Sharma, Ram Avtar Bumb, Narendra Singh Negi, V Ramesh, Poonam Salotra
BACKGROUND: Leishmaniasis is a spectrum of diseases with great relevance to public health. Conventional diagnostic methods are time consuming, needing trained personnel. A robust, rapid and cost effective diagnostic test is warranted for on-time diagnosis and field application. METHODS: We have developed a loop mediated isothermal amplification (LAMP) assay with primers (n = 6) based on Leishmania donovani kDNA for detection of Leishmania infection, using a closed tube to prevent cross-contamination...
March 23, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28330752/prevalence-and-distribution-of-covert-mortality-nodavirus-cmnv-in-cultured-crustacean
#19
Qingli Zhang, Tingting Xu, Xiaoyuan Wan, Shuang Liu, Xiuhua Wang, Xiaoping Li, Xuan Dong, Bing Yang, Jie Huang
An emerging covert mortality nodavirus (CMNV) was proved to be the infectious agent of shrimp viral covert mortality disease (VCMD). Prevalence and distribution of CMNV were investigated by using the methods of reverse transcription loop-mediated isothermal amplification (RT-LAMP), nested reverse transcription PCR, gene sequencing, histopathology, in situ RNA hybridization (ISH) and transmission electron microscope (TEM) in this study. RT-LAMP results showed that CMNV positive samples appeared in the cultured crustaceans including Litopenaeus vannamei, Fenneropenaeus chinensis, Marsupenaeus japonicus, Penaeus monodon, and Macrobrachium rosenbergii, and mostly distributed the coastal provinces in China...
March 19, 2017: Virus Research
https://www.readbyqxmd.com/read/28324245/comparison-of-dna-microarray-loop-mediated-isothermal-amplification-lamp-and-real-time-pcr-with-dna-sequencing-for-identification-of-fusarium-spp-obtained-from-patients-with-hematologic-malignancies
#20
Marcela de Souza, Tetsuhiro Matsuzawa, Kanae Sakai, Yasunori Muraosa, Luzia Lyra, Ariane Fidelis Busso-Lopes, Anna Sara Shafferman Levin, Angélica Zaninelli Schreiber, Yuzuru Mikami, Tohoru Gonoi, Katsuhiko Kamei, Maria Luiza Moretti, Plínio Trabasso
The performance of three molecular biology techniques, i.e., DNA microarray, loop-mediated isothermal amplification (LAMP), and real-time PCR were compared with DNA sequencing for properly identification of 20 isolates of Fusarium spp. obtained from blood stream as etiologic agent of invasive infections in patients with hematologic malignancies. DNA microarray, LAMP and real-time PCR identified 16 (80%) out of 20 samples as Fusarium solani species complex (FSSC) and four (20%) as Fusarium spp. The agreement among the techniques was 100%...
March 21, 2017: Mycopathologia
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