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https://www.readbyqxmd.com/read/28221922/contribution-of-selective-conditions-to-microbial-competition-in-four-listeria-selective-enrichment-formulations
#1
Ashley L Keys, Anthony D Hitchins, R Derike Smiley
Microbial competition during selective enrichment negatively affects Listeria monocytogenes populations and may hinder the subsequent detection or recovery of this organism. Competition assays among 10 selected strains of Listeria and Citrobacter braakii were performed in buffered Listeria enrichment broth, 3-(N-morpholino)propanesulfonic acid-buffered Listeria enrichment broth, University of Vermont medium-modified Listeria enrichment broth, and Fraser broth. The individual contributions of each selective agent in these media were also assessed, as well as the contribution of incubation temperature...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221887/soil-type-soil-moisture-and-field-slope-influence-the-horizontal-movement-of-salmonella-enterica-and-citrobacter-freundii-from-floodwater-through-soil
#2
Mary Theresa Callahan, Shirley A Micallef, Robert L Buchanan
Pathogens in soil are readily mobilized by infiltrating water to travel downward through the soil. However, limited data are available on the horizontal movement of pathogens across a field. This study used a model system to evaluate the influence of soil type, initial soil moisture content, and field slope on the movement of Salmonella enterica serovar Newport across a horizontal plane of soil under flooding conditions. Three soil types of varying clay content were moistened to 40, 60, or 80% of their maximum water-holding capacities and flooded with water containing 6 log CFU/ml Salmonella Newport and Citrobacter freundii , the latter being evaluated as a potential surrogate for S...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28202619/exopolysaccharide-from-bacillus-subtilis-induces-anti-inflammatory-m2-macrophages-that-prevent-t-cell-mediated-disease
#3
Mallory L Paynich, Sara E Jones-Burrage, Katherine L Knight
Commensal bacteria contribute to immune homeostasis in the gastrointestinal tract; however, the underlying mechanisms for this are not well understood. A single dose of exopolysaccharide (EPS) from the probiotic spore-forming bacterium Bacillus subtilis protects mice from acute colitis induced by the enteric pathogen Citrobacter rodentium Adoptive transfer of macrophage-rich peritoneal cells from EPS-treated mice confers protection from disease to recipient mice. In vivo, EPS induces development of anti-inflammatory M2 macrophages in a TLR4-dependent manner, and these cells inhibit T cell activation in vitro and in C...
February 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28192483/development-of-a-high-throughput-method-to-evaluate-serum-bactericidal-activity-using-bacterial-atp-measurement-as-survival-readout
#4
Francesca Necchi, Allan Saul, Simona Rondini
Serum Bactericidal Activity (SBA) assay is the method of choice to evaluate the complement-mediated functional activity of both infection- and vaccine-induced antibodies. To perform a typical SBA assay, serial dilutions of sera are incubated with target bacterial strains and complement. The conventional SBA assay is based on plating on agar the SBA reaction mix and counting the surviving bacterial colony forming units (CFU) at each serum dilution. Even with automated colony counting, it is labor-intensive, time-consuming and not amenable for large-scale studies...
2017: PloS One
https://www.readbyqxmd.com/read/28178629/plasma-methionine-depletion-and-pharmacokinetic-properties-in-mice-of-methionine-%C3%AE-lyase-from-citrobacter-freundii-clostridium-tetani-and-clostridium-sporogenes
#5
E A Morozova, N V Anufrieva, D Zh Davydov, M V Komarova, I N Dyakov, A N Rodionov, T V Demidkina, V S Pokrovsky
PK studies were carried out after a single i.v. administration of 500 and 1000 U/kg by measuring of MGL activity in plasma samples. L-methionine concentration was measured by mass spectrometry. After single i.v. injection of 500U/kg the circulating T1/2 of enzymes in mice varies from 73 to 123min. The AUC0-tinf values determined for MGL 500U/kg from C. freundii, C. tetani and C. sporogenes are 8.21±0.28, 9.04±0.33 and 13.88±0.39U/(ml×h), respectively. Comparison of PK parameters of three MGL sources in the dose of 500U/kg indicated the MGL C...
February 5, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28176201/lactobacillus-delbrueckii-subsp-lactis-strain-cidca-133-stimulates-murine-macrophages-infected-with-citrobacter-rodentium
#6
Ayelén A Hugo, Ivanna S Rolny, David Romanin, Pablo F Pérez
Citrobacter rodentium is a specific murine enteropathogen which causes diarrheal disease characterized by colonic hyperplasia and intestinal inflammation. Recruitment of neutrophils and macrophages constitute a key step to control the infection. Since modulation of the activity of professional phagocytic cells could contribute to improve host´s defences against C. rodentium, we investigated the effect of Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) on the interaction between murine macrophages (RAW 264...
March 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28163398/%C3%AE%C2%BA-carrageenan-enhances-lipopolysaccharide-induced-interleukin-8-secretion-by-stimulating-the-bcl10-nf-%C3%AE%C2%BAb-pathway-in-ht-29-cells-and-aggravates-c-freundii-induced-inflammation-in-mice
#7
Wei Wu, Zhanghe Zhen, Tingting Niu, Xiaojuan Zhu, Yuli Gao, Jiangyan Yan, Yu Chen, Xiaojun Yan, Haimin Chen
Background. The dietary usage of carrageenan as common food additive has increased observably over the last 50 years. But there is substantial controversy about its safety. Methods. We investigated whether the κ-carrageenan could enhance lipopolysaccharide-induced IL-8 expression by studying its actions on the TLR4-NF-κB pathway. The aggravating effect of κ-carrageenan on Citrobacter freundii DBS100-induced intestinal inflammation was also investigated in a mouse model. Results. Our data show that κ-carrageenan pretreatment promoted LPS-induced IL-8 expression in HT-29 cells...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28159898/nlrx1-regulates-effector-and-metabolic-functions-of-cd4-t-cells
#8
Andrew Leber, Raquel Hontecillas, Nuria Tubau-Juni, Victoria Zoccoli-Rodriguez, Matthew Hulver, Ryan McMillan, Kristin Eden, Irving C Allen, Josep Bassaganya-Riera
Nucleotide oligomerization domain-like receptor X1 (NLRX1) has been implicated in viral response, cancer progression, and inflammatory disorders; however, its role as a dual modulator of CD4(+) T cell function and metabolism has not been defined. The loss of NLRX1 results in increased disease severity, populations of Th1 and Th17 cells, and inflammatory markers (IFN-γ, TNF-α, and IL-17) in mice with dextran sodium sulfate-induced colitis. To further characterize this phenotype, we used in vitro CD4(+) T cell-differentiation assays and show that NLRX1-deficient T cells have a greater ability to differentiate into an inflammatory phenotype and possess greater proliferation rates...
February 3, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28155661/functional-analysis-for-gut-microbes-of-the-brown-tree-frog-polypedates-megacephalus-in-artificial-hibernation
#9
Francis Cheng-Hsuan Weng, Yi-Ju Yang, Daryi Wang
BACKGROUND: Annual hibernation is an adaptation that helps many animals conserve energy during food shortage in winter. This natural cycle is also accompanied by a remodeling of the intestinal immune system, which is an aspect of host biology that is both influenced by, and can itself influence, the microbiota. In amphibians, the bacteria in the intestinal tract show a drop in bacterial counts. The proportion of pathogenic bacteria is greater in hibernating frogs than that found in nonhibernating frogs...
December 22, 2016: BMC Genomics
https://www.readbyqxmd.com/read/28155242/sensory-neuron-regulation-of-gastrointestinal-inflammation-and-bacterial-host-defence
#10
N Y Lai, K Mills, I M Chiu
Sensory neurons in the gastrointestinal tract have multifaceted roles in maintaining homeostasis, detecting danger and initiating protective responses. The gastrointestinal tract is innervated by three types of sensory neurons: dorsal root ganglia, nodose/jugular ganglia and intrinsic primary afferent neurons. Here, we examine how these distinct sensory neurons and their signal transducers participate in regulating gastrointestinal inflammation and host defence. Sensory neurons are equipped with molecular sensors that enable neuronal detection of diverse environmental signals including thermal and mechanical stimuli, inflammatory mediators and tissue damage...
February 2, 2017: Journal of Internal Medicine
https://www.readbyqxmd.com/read/28153975/citrobacter-youngae-and-pantoea-agglomerans-peritonitis-in-a-peritoneal-dialysis-patient
#11
(no author information available yet)
No abstract text is available yet for this article.
January 2017: Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis
https://www.readbyqxmd.com/read/28149295/marine-bivalve-mollusks-as-possible-indicators-of-multidrug-resistant-escherichia-coli-and-other-species-of-the-enterobacteriaceae-family
#12
Didrik H Grevskott, Cecilie S Svanevik, Marianne Sunde, Astrid L Wester, Bjørn T Lunestad
The mechanisms for the development and spread of antibacterial resistance (ABR) in bacteria residing in environmental compartments, including the marine environment, are far from understood. The objective of this study was to examine the ABR rates in Escherichia coli and other Enterobacteriaceae isolates obtained from marine bivalve mollusks collected along the Norwegian coast during a period from October 2014 to November 2015. A total of 549 bivalve samples were examined by a five times three tube most probable number method for enumeration of E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28143582/gut-symbiont-enhances-insecticide-resistance-in-a-significant-pest-the-oriental-fruit-fly-bactrocera-dorsalis-hendel
#13
Daifeng Cheng, Zijun Guo, Markus Riegler, Zhiyong Xi, Guangwen Liang, Yijuan Xu
BACKGROUND: Symbiotic bacteria affect insect physiology and ecology. They may also mediate insecticide resistance within their hosts and thereby impact pest and vector control practices. Here, we document a novel mechanism of insecticide resistance in which a gut symbiont of the tephritid pest fruit fly Bactrocera dorsalis enhances resistance to the organophosphate insecticide trichlorphon. RESULTS: We demonstrated that the gut symbiont Citrobacter sp. (CF-BD) plays a key role in the degradation of trichlorphon...
February 1, 2017: Microbiome
https://www.readbyqxmd.com/read/28128639/evaluation-of-three-bacterial-identification-systems-for-species-identification-of-bacteria-isolated-from-bovine-mastitis-and-bulk-tank-milk-samples
#14
Emily Savage, Shubhada Chothe, Valerie Lintner, Traci Pierre, Tammy Matthews, Subhashinie Kariyawasam, Dawn Miller, Deepanker Tewari, Bhushan Jayarao
A study was conducted to evaluate Sensititre(®) Automated Reading and Incubation System 2x System (ARIS), API(®) (API), and Bruker MALDI-TOF MS (MALDI) bacterial species identification systems using 132 diverse bacterial isolates from bovine milk samples and bulk tank milk received at the Penn State Animal Diagnostic Laboratory. The results were compared with 16S rRNA gene sequence analysis, which served as the reference method for species identification. The ARIS, API, and MALDI identified 0%, 40%, and 33...
January 27, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28124783/diversity-of-culturable-bacterial-communities-in-the-intestinal-tracts-of-goldfish-carassius-auratus-and-their-ability-to-produce-n-acyl-homoserine-lactone
#15
Haruo Sugita, Shun Kitao, Satoshi Narisawa, Ryosuke Minamishima, Shiro Itoi
Intestinal bacteria isolated from goldfish (Carassius auratus) were identified based on 16 ribosomal RNA (rRNA) gene sequences and screened for their ability to produce N-acyl homoserine lactone (AHL), an autoinducer of the quorum sensing (QS) system. The 230 aerobes/facultative anaerobes that were isolated comprised members of the genera Aeromonas (184 isolates), Citrobacter (11), Enterobacter (2), Shewanella (28), Vagococcus (1), and Vibrio (4). Among these genera, the two most abundant species were Aeromonas veronii (163 isolates) and Shewanella xiamenensis (27)...
January 26, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28121036/effect-of-preoperative-biliary-drainage-on-bacterial-flora-in-bile-of-patients-with-periampullary-cancer
#16
F Scheufele, L Aichinger, C Jäger, I E Demir, S Schorn, M Sargut, M Erkan, J Kleeff, H Friess, G O Ceyhan
BACKGROUND: Patients with obstructive jaundice due to periampullary tumours may undergo preoperative biliary drainage (PBD). The effect of PBD on the microbiome of the biliary system and on postoperative outcome remains unclear. METHODS: A single-centre retrospective study of patients with obstructive jaundice due to periampullary cancer, treated between July 2007 and July 2015, was undertaken. Intraoperative bile samples were obtained for microbiological analysis after transection of the common bile duct...
January 2017: British Journal of Surgery
https://www.readbyqxmd.com/read/28110033/purification-characterization-and-functional-analysis-of-the-immune-molecule-lectin-from-the-haemolymph-of-blue-swimmer-crab-portunus-pelagicus-and-their-antibiofilm-properties
#17
Sangily Jayanthi, Ramachandran Ishwarya, Mahalingam Anjugam, Arokiadhas Iswarya, Sivashanmugam Karthikeyan, Baskaralingam Vaseeharan
The present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab Portunus pelagicus (Pp-Lec). The Pp-Lec was purified by affinity chromatography with mannose coupled sepharose CL-4B column and it exhibits single band with a molecular weight of 155 kDa in SDS-PAGE. The surface morphology of purified Pp-Lec displays the homogeneous nature of protein. A distinct peak with a retention time of 3.3 min was appeared in high performance liquid chromatography (HPLC) and X-ray diffraction (XRD) analysis expresses a single peak at 31...
January 18, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28105684/bacterial-microcompartment-directed-polyphosphate-kinase-promotes-stable-polyphosphate-accumulation-in-e-coli
#18
Mingzhi Liang, Stefanie Frank, Heinrich Lünsdorf, Martin J Warren, Michael B Prentice
Processes for the biological removal of phosphate from wastewater rely on temporary manipulation of bacterial polyphosphate levels by phased environmental stimuli. In E. coli polyphosphate levels are controlled via the polyphosphate-synthesizing enzyme polyphosphate kinase (PPK1) and exopolyphosphatases (PPX and GPPA), and are temporarily enhanced by PPK1 overexpression and reduced by PPX overexpression. We hypothesised that partitioning PPK1 from cytoplasmic exopolyphosphatases would increase and stabilise E...
January 20, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28096401/ornithine-decarboxylase-regulates-m1-macrophage-activation-and-mucosal-inflammation-via-histone-modifications
#19
Dana M Hardbower, Mohammad Asim, Paula B Luis, Kshipra Singh, Daniel P Barry, Chunying Yang, Meredith A Steeves, John L Cleveland, Claus Schneider, M Blanca Piazuelo, Alain P Gobert, Keith T Wilson
Macrophage activation is a critical step in host responses during bacterial infections. Ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine metabolism, has been well studied in epithelial cells and is known to have essential roles in many different cellular functions. However, its role in regulating macrophage function during bacterial infections is not well characterized. We demonstrate that macrophage-derived ODC is a critical regulator of M1 macrophage activation during both Helicobacter pylori and Citrobacter rodentium infection...
January 31, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28094878/characterization-of-cellulose-degrading-bacteria-from-the-larval-gut-of-the-white-grub-beetle-lepidiota-mansueta-coleoptera-scarabaeidae
#20
Gautam Handique, Amrita Phukan, Badal Bhattacharyya, Abu Adil Lutful Haque Baruah, Syed Wasifur Rahman, Rajen Baruah
The goal of this study is to identify and characterize the cellulose degrading microorganisms in the larval gut of the white grub beetle, Lepidiota mansueta. Thirty bacterial strains were isolated and tested for cellulolytic activity using soluble carboxymethyl cellulose (CMC) degrading assays. Of these strains, five (FGB1, FB2, MB1, MB2, and HB1) degrade cellulose. Cellulolytic activity was determined based on formation of clear zone and cellulolytic index on CMC plate media. The highest cellulolytic index (2...
January 17, 2017: Archives of Insect Biochemistry and Physiology
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