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Lang Yang, Peihan Li, Beibei Liang, Xiaofeng Hu, Jinhui Li, Jing Xie, Chaojie Yang, Rongzhang Hao, Ligui Wang, Leili Jia, Peng Li, Shaofu Qiu, Hongbin Song
The emergence of carbapenemase-producing Citrobacter freundii poses a significant threat to public health worldwide. Here, we reported a C. freundii strain CWH001 which was resistant to all tested antimicrobials except tetracycline. Whole genome sequencing and analysis were performed. The strain, which belonged to a new sequence type ST139, showed close relationship with other foreign C. freundii strains through phylogenetic analysis. A novel variant of the intrinsic blaCMY gene located on the chromosome was identified and designated as blaCMY-152 ...
July 13, 2018: Scientific Reports
T Bandyopadhyay, A Kumar, A Saili, V S Randhawa
OBJECTIVE: The aim of this study is to investigate etiological agents, patterns of antimicrobial resistance and predictors of mortality in culture proven neonatal sepsis. METHOD: This is a twenty-four month retrospective cohort study of infants with culture proven sepsis. Demographic data, type of isolates and its sensitivity pattern were recorded. Multidrug resistant gram-negative isolates were defined as resistance to any three of five antibiotic classes: extended-spectrum cephalosporins, carbapenems, aminoglycosides, fluoroquinolones and piperacillin-tazobactam...
2018: Journal of Neonatal-perinatal Medicine
N Ueoka, A Kouzuma, K Watanabe
Exoelectrogens play central roles in microbial fuel cells and other bioelectrochemical systems (BESs), yet their physiological diversity remains largely elusive due to the lack of efficient methods for the isolation from naturally occurring microbiomes. The present study developed an electrode plate-culture (EPC) method that facilitates selective colony formation by exoelectrogens and used it for isolating them from an exoelectrogenic microbiome enriched from paddy-field soil. In an EPC device, the surface of solidified agarose medium was spread with a suspension of a microbiome and covered with a transparent fluorine doped tin oxide (FTO) electrode (poised at 0 V vs...
June 22, 2018: Bioelectrochemistry
Michał Dmowski, Marcin Gołębiewski, Izabela Kern-Zdanowicz
Plasmid conjugative transfer systems comprise type IV secretion systems (T4SS) coupled to DNA processing and replication. The T4SSs are divided into two phylogenetic subfamilies, namely, IVA and IVB or based on the phylogeny of the VirB4 ATPase into eight groups. The conjugation system of the IncM group plasmid pCTX-M3, from Citrobacter freundii , is classified in the IVB subfamily and in the MPFI group, as are the conjugation systems of IncI1 group plasmids. Although the majority of the conjugative genes of the IncM and IncI1 plasmids display conserved synteny, there are several differences...
July 9, 2018: Journal of Bacteriology
Yong-Chun Liu, Ri-Ming Huang, Jie Bao, Ke-Yue Wu, Heng-Yu Wu, Xiang-Yang Gao, Xiao-Yong Zhang
The microbes associated with black corals remain poorly studied. The present study is the first attempt to investigate microbial community structure in the black corals Antipathes ceylonensis and A. dichotoma from the South China Sea by using high-throughput Illumina sequencing. A total of 52 bacterial and 3 archaeal phyla were recovered in this study, suggesting the black corals harboured highly diverse microbial communities. Among the 55 microbial phyla, Proteobacteria, Firmicutes, Bacteroidetes, Chloroflexi, Acidobacteria and Actinobacteria dominated in the two black corals from the South China Sea...
June 30, 2018: FEMS Microbiology Letters
Siraj Anwar, Uma Bhandari, Bibhu Prasad Panda, Kiran Dubey, Washim Khan, Sayeed Ahmad
Gut microbiota based metabolism of choline produces trimethylamine (TMA) which is further converted to a pro-atherosclerotic metabolite, trimethylamine-N-oxide (TMAO) by flavin monooxygenase (FMO3 ). Trigonelline from the plant Trigonella foenum-graecum has been reported for the treatment of CVD. Aim of the present study was to check the effect of trigonelline on the gut microbiota based conversion of TMA to TMAO. Trigonelline was isolated from hydroalcoholic extract of seeds of Trigonella foenum-graecum. The isolated trigonelline was characterized through TLC and UPLC-MS...
June 28, 2018: Journal of Pharmaceutical and Biomedical Analysis
Natalia Walczak, Krzysztof Puk, Leszek Guz
Introduction: Ornamental fish can suffer from different bacterial diseases. Among them the most prevalent are infections caused by Aeromonas, Shewanella, Citrobacter, Plesiomonas, Edwardsiella , and Pseudomonas . But there is a broad spectrum of rarely identified bacteria which may be causative agents of diseases. The aim of the study was to determine the species of bacteria pathogenic for fish which are prevalent in aquariums. Material and Methods: Bacteria were isolated from infected ornamental fish from pet shops and private aquariums in the Lublin region in 2015 and classified to species using MALDI-TOF MS...
December 2017: Journal of Veterinary Research
Isabelle C Arnold, Mariela Artola-Borán, Paulino Tallón de Lara, Andreas Kyburz, Christian Taube, Karen Ottemann, Maries van den Broek, Shida Yousefi, Hans-Uwe Simon, Anne Müller
Eosinophils are predominantly known for their contribution to allergy. Here, we have examined the function and regulation of gastrointestinal eosinophils in the steady-state and during infection with Helicobacter pylori or Citrobacter rodentium We find that eosinophils are recruited to sites of infection, directly encounter live bacteria, and activate a signature transcriptional program; this applies also to human gastrointestinal eosinophils in humanized mice. The genetic or anti-IL-5-mediated depletion of eosinophils results in improved control of the infection, increased inflammation, and more pronounced Th1 responses...
July 3, 2018: Journal of Experimental Medicine
Bo-Qiang Wang, Chen-Yang Li, Wei Lu, Hao Fan, Dong-Zhi Zhang, Zhi Wang, Cong Lü, Fu-Dong Shen
In this study, the dynamic variation of the structure, functionality and biodiversity of indigenous microorganism during the CO2 -brine-sandstone interaction process was investigated using MiSeq sequencing techniques. The results indicated that some kinds of indigenous microorganisms could grow well under the extreme condition induced by CO2 -injection. After injection of CO2 , the species of indigenous microorganisms tended to be single and the relative abundance of Proteobacteria reached up to 99.77% after 6 months...
July 8, 2017: Huan Jing Ke Xue= Huanjing Kexue
Arshi Taj, Anjum Shamim, Shoiab Bashir Khanday, Mohamad Ommid
Background: Nosocomial infection presents with high mortality rate, and it remains a diagnostic and treatment challenge for health-care providers, with developing countries having the highest incidence and mortality rates.[1]. Aim: The present study was undertaken to evaluate prevalence of commonly isolated nosocomial organisms in patients admitted in Surgical Intensive Care Unit (ICU) in Government Medical College Srinagar. Materials and Methods: The study was proposed to be conducted in surgical ICU of Shri Maharaja Hari Singh - a Tertiary Care Hospital in Jammu and Kashmir (India) from March 2015 to March 2016...
April 2018: International Journal of Critical Illness and Injury Science
Natallia Varankovich, Alexander Grigoryan, Kirsty Brown, G Douglas Inglis, Richard R E Uwiera, Michael T Nickerson, Darren R Korber
The efficacy of two strains of Lactobacillus probiotics immobilized in pea protein isolate (PPI) - alginate microcapsules was assessed using a mouse model of Citrobacter rodentium-induced colitis. Mice were fed diets supplemented with freeze-dried probiotics, probiotic-containing PPI-alginate microcapsules or PPI-alginate microcapsules containing no probiotics. Half the mice were gavaged with C. rodentium 2 weeks after initiation of feeding. Daily monitoring of disease symptoms and body weights were undertaken...
June 29, 2018: Canadian Journal of Microbiology
Valerie Osieka, Mirjam Grobbel, Silvia Schmoger, Claudia A Szentiks, Alexandra Irrgang, Annemarie Käsbohrer, Bernd-Alois Tenhagen, Jens A Hammerl
Here, we announce the genome of an extended-spectrum β-lactamase-producing Citrobacter freundii strain isolated from the cecum of a house sparrow that was found dead in Berlin-Lichtenberg, Germany, in 2017. This isolate exhibits increased MICs for several antimicrobials and a comprehensive set of acquired resistance determinants potentially involved in horizontal gene transfer.
June 28, 2018: Genome Announcements
Chih-Ming Chen, Mei Huang, Hwa-Jene Wu, Ming-Kai Guo, Lii-Tzu Wu
A clinical isolate of Citrobacter freundii, JA99 obtained from bile culture of a Taiwanese patient, was found to produce a plasmid-encoded β-lactamase that conferred resistance to oxyimino-cephalosporins and cephamycins. Resistance arising from production of the β-lactamase could be transferred by conjugation with an IncW plasmid pJA99 into Escherichia coli J53. The substrate and inhibition profiles of this enzyme resembled those of the AmpC β-lactamase. The resistance gene of pJA99, cloned and expressed in E...
June 23, 2018: International Journal of Antimicrobial Agents
Jamila A D Jones, R G Kerr, B A Haltli, Winston F Tinto
Cellulolytic bacteria that produce cellulases, which are active over a range of pH and temperatures, can be used to catalyze hydrolysis of pretreated lignocellulosic material. This is important in the production of second generation biofuels among other biotechnological applications. In this investigation, bacteria isolated from sugarcane bagasse were identified as strains of Enterobacter xiangfangensis , Serratia rubidaea , Klebsiella pneumoniae and a novel species of Citrobacter designated Citrobacter sp...
June 2018: Heliyon
Olajumoke R Akinbami, Samson Olofinsae, Funmilola A Ayeni
A serious concern is arising on the coexistence of extended-spectrum beta-lactamase (ESBL) and plasmid mediated quinolone resistance (PMQR) producing bacteria in animal husbandry, which could be transferred to humans, especially in strains that may not be routinely screened for resistance. This study therefore tested the prevalence of ESBL and PMQR genes in selected bacteria isolated from poultry faeces. Faecal droppings of birds were collected from 11 farms in five states in South Western Nigeria. Bacteria were isolated from the samples on cefotaxime supplemented plates and identified with MALDI-TOF...
2018: PeerJ
Katie E Barber, Jason M Pogue, Henderson D Warnock, Robert A Bonomo, Keith S Kaye
Background: 'Last-line' antimicrobial usage has promoted the emergence of MDR bacteria. Production of Klebsiella pneumoniae carbapenemases (KPCs) is increasingly common and leads to resistance to most antimicrobials. However, ceftazidime/avibactam demonstrates activity against KPC-producing strains. Ceftazidime/avibactam in the empirical setting remains unknown. Methods: Strains underwent genetic analysis evaluating blaKPC presence/production and MICs were determined...
June 22, 2018: Journal of Antimicrobial Chemotherapy
D X Vega-Manriquez, R P Dávila-Arrellano, C A Eslava-Campos, E Salazar Jiménez, A C Negrete-Philippe, R Raigoza-Figueras, F A Muñoz-Tenería
Anthropogenic activities, predation, and diseases have contributed to a decrease in the sea turtle population in recent years. Ulcerative stomatitis is a condition that occurs in both wild and captive populations. The etiology of this condition is associated with bacteria such as E. coli, Citrobacter diversus, Klebsiella spp., Pseudomonas spp., Flavobacter calcoaceticus, Staphylococcus spp., and Flavobacterium spp. Some of these microorganisms are part of the oral microbiota of turtles, but alterations in the immune response can disturb the homeostatic relationship and cause an increase in the population of microorganisms, which in turn can cause disease...
June 22, 2018: Veterinary Research Communications
Branka Bedenić, Mia Slade, Lidija Žele Starčević, Sanda Sardelić, Mirna Vranić-Ladavac, Ana Benčić, Vlasta Zujić Atalić, Maja Bogdan, Marina Bubonja-Šonje, Maja Tomić-Paradžik, Tatjana Tot, Amarela Lukić-Grlić, Domagoj Drenjančević, Dijana Varda-Brkić, Daniela Bandić-Pavlović, Slobodan Mihaljević, Gernot Zarfel, Marija Gužvinec, Rick Conzemius, Ivan Barišić, Arjana Tambić-Andraševic
PURPOSE: A dramatic increase in OXA-48 β-lactamase was observed recently not only in large hospital centres, but also in smaller suburban hospital centres in geographic areas bordering Croatia. The aim of the study was to analyse the epidemiology, the mechanisms of antibiotic resistance and the routes of spread of OXA-48 carbapenemase in Croatia. METHODS: Carbapenemase and other β-lactamase and fluoroquinolone resistance genes were detected by PCR and sequencing...
June 21, 2018: Journal of Medical Microbiology
Roya Chegene Lorestani, Alisha Akya, Azam Elahi, Yazdan Hamzavi
Background and Objectives: Integrons play a major role in the transmission and accumulation of resistance factors in multidrug resistant bacteria. This study was aimed to evaluate the gene cassettes of class I integron and antimicrobial resistance in isolates of Citrobacter with multidrug resistance (MDR). Materials and Methods: Ninety isolates of Citrobacter spp. were collected from the largest hospital in Kermanshah, Iran. Antimicrobial resistance patterns were determined using disc diffusion method...
February 2018: Iranian Journal of Microbiology
Alain P Gobert, Nicole T Al-Greene, Kshipra Singh, Lori A Coburn, Johanna C Sierra, Thomas G Verriere, Paula B Luis, Claus Schneider, Mohammad Asim, Margaret M Allaman, Daniel P Barry, John L Cleveland, Christina E Destefano Shields, Robert A Casero, M Kay Washington, M Blanca Piazuelo, Keith T Wilson
Polyamines have been implicated in numerous biological processes, including inflammation and carcinogenesis. Homeostatic regulation leads to interconversion of the polyamines putrescine and the downstream metabolites spermidine and spermine. The enzyme spermine oxidase (SMOX), which back-converts spermine to spermidine, contributes to regulation of polyamine levels, but can also have other effects. We have implicated SMOX in gastric inflammation and carcinogenesis due to infection by the pathogen Helicobacter pylori ...
2018: Frontiers in Immunology
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