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Jerusalem artichokes

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https://www.readbyqxmd.com/read/29157907/expression-and-purification-of-plant-fructan-exohydrolases-and-their-potential-applications-in-fructose-production
#1
Wenyue Zhan, Lijin Jin, Jiao Jiao, Xi Zhang, Yan Zhang, Haiyan Zhao, Mingxiang Liang
Inulinases from microorganisms have been extensively studied for their role in the production of fructose from fructan. Fructan can also be hydrolyzed by plant fructan exohydrolases (FEHs), but these enzymes have not been used to produce fructose commercially. Two Ht1-FEHs (Ht1-FEH I and Ht1-FEH II) were recently characterized in Jerusalem artichoke. In this study, we cloned the third member of the Ht1-FEH family in Jerusalem artichoke (i.e., Ht1-FEH III). When heterologously expressed in Pichia pastoris X-33, Ht1-FEH III not only demonstrated hydrolysis activity towards β (2, 1)-linked fructans and β (2, 6)-linked levan, but also towards sucrose...
November 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29117735/antioxidant-and-%C3%AE-glucosidase-inhibitory-ingredients-identified-from-jerusalem-artichoke-flowers
#2
Yan-Ming Wang, Jian-Qiang Zhao, Jun-Li Yang, Pema Tsering Idong, Li-Juan Mei, Yan-Duo Tao, Yan-Ping Shi
Jerusalem artichoke (JA, Helianthus tuberosus L.) has been researched extensively due to its wide range of uses, but there are limited studies on its flowers. In this study, we report the first detailed phytochemical study on JA flowers, which yielded 21 compounds. Compound 4 was identified as a major water-soluble yellow pigment of JA flowers. In addition, the methanol extract of JA flowers and the isolates were evaluated for their antioxidant and α-glucosidase inhibitory activities. Among the tested compounds, compound 13 showed the strongest ABTS(+) free radical scavenging activity with SC50 value of 2...
November 9, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29073305/-topinambour-nutritional-and-medical-properties-of-the-jerusalem-artichoke-helianthus-tuberoses-l
#3
REVIEW
Michalina Horochowska, Elżbieta Kołeczek, Zygmunt Zdrojewicz, Jacek Jagiełło, Karolina Pawlus
Topinambour (Helianthus tuberosus L.), also known as the Jerusalem artichoke is a plant, which origins from North America. In XVII century it was brought to the Europe. For ages it was cultivated due to edible tuber and its healing properties.The aim of the article is to present medical properties and application of topinmbour in patient's diet. Many studies were performed on animals to specify medical properties of topinambour. Results show, that topinambour lowers plasma glucose, total cholesterol and triglyceride levels...
2017: Pediatric Endocrinology, Diabetes, and Metabolism
https://www.readbyqxmd.com/read/28899777/jerusalem-artichoke-enriched-diet-on-growth-performance-immuno-hematological-changes-and-disease-resistance-against-aeromonas-hydrophila-in-asian-seabass-lates-calcarifer
#4
Sajjad Syed Raffic Ali, Kondusamy Ambasankar, Mohamed Saiyad Musthafa, Ramasamy Harikrishnan
A 45 days feeding trial was conducted to study the effect of Jerusalem artichoke (JA) on growth performance, body composition, biochemical, immuno-hematological parameters and disease resistance in Asian seabass (Lates calcarifer) fingerlings against Aeromonas hydrophila. JA was supplemented at three different levels viz., control 0, 5, 10, and 20 g kg(-1) in the commercial diet (403 g kg(-1)protein and 89 g kg (-1)lipid) in L. calcarifer. The results showed that there were no significant (P > 0...
September 9, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28867090/nutrients-and-natural-toxic-substances-in-commonly-consumed-jerusalem-artichoke-helianthus-tuberosus-l-tuber
#5
Kunchit Judprasong, Nidthida Archeepsudcharit, Kedsiri Chantapiriyapoon, Pharrunrat Tanaviyutpakdee, Piya Temviriyanukul
This study determined nutrients, chemical contaminants, (insecticide residues and heavy metals), and natural toxic substances (nitrate, nitrite, cyanide, oxalate, phytate, and trypsin inhibitor) in tubers of Jerusalem artichokes-Kaentawan in the Thai language-grown in four major provinces in Thailand. They were purchased, prepared, homogenized, and freeze-dried for further analysis using standard methods. All Kaentawan samples contained considerable amounts of fructans and dietary fiber (15.4±0.2gand3.2±0...
January 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28833484/microbial-production-of-mannitol-by-lactobacillus-brevis-3-a5-from-concentrated-extract-of-jerusalem-artichoke-tubers
#6
Hailong Cao, Min Yue, Gang Liu, Yuguang Du, Heng Yin
In the present study, the conversion of the extract of Jerusalem artichoke tubers for mannitol production by Lactobacillus brevis 3-A5 was investigated. When the bacterium utilized enzymatic hydrolysates of Jerusalem artichoke extract as the main substrates in batch fermentation, the significant decrease in mannitol productivity was observed when the initial concentration of reducing sugar increased. Then, a strategy of continuous fed-batch fermentation was adopted for improving mannitol production with enzymatic hydrolysates of Jerusalem artichoke extract as main substrates...
August 17, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28801602/fermentative-hydrogen-production-from-jerusalem-artichoke-by-clostridium-tyrobutyricum-expressing-exo-inulinase-gene
#7
Ling Jiang, Qian Wu, Qing Xu, Liying Zhu, He Huang
Clostridium tyrobutyricum ATCC25755 has been reported as being able to produce significant quantities of hydrogen. In this study, the exo-inulinase encoding gene cloned from Paenibacillus polymyxa SC-2 was into the expression plasmid pSY6 and expressed in the cells of C. tyrobutyricum. The engineered C. tyrobutyricum strain efficiently fermented the inulin-type carbohydrates from Jerusalem artichoke, without any pretreatment being necessary for the production of hydrogen. A comparatively high hydrogen yield (3...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28731563/improving-fructose-utilization-and-butanol-production-by-clostridium-acetobutylicum-via-extracellular-redox-potential-regulation-and-intracellular-metabolite-analysis
#8
Li-Jie Chen, You-Duo Wu, Chuang Xue, Feng-Wu Bai
Jerusalem artichoke (JA) can grow well in marginal lands with high biomass yield, and thus is a potential energy crop for biorefinery. The major biomass of JA is from tubers, which contain inulin that can be easily hydrolyzed into a mixture of fructose and glucose, but fructose utilization for producing butanol as an advanced biofuel is poor compared to glucose-based ABE fermentation by Clostridium acetobutylicum. In this article, the impact of extracellular redox potential (ORP) on the process is studied using a mixture of fructose and glucose to simulate the hydrolysate of JA tubers...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28647180/helianthus-tuberosus-jerusalem-artichoke-tubers-improve-glucose-tolerance-and-hepatic-lipid-profile-in-rats-fed-a-high-fat-diet
#9
Naoto Okada, Shinya Kobayashi, Kouta Moriyama, Kohsuke Miyataka, Shinji Abe, Chiemi Sato, Kazuyoshi Kawazoe
OBJECTIVES: To analyze the effects of feeding Helianthus tuberosus (HT) tubers on glucose tolerance and lipid profile in rats fed a high-fat diet (HFD). METHODS: A normal HFD or HFD including 10 w/w% HT tubers (HFD + HT) was fed to F334/Jcl rats. After 10 weeks, organ weights, glucose tolerance, and lipid profile were analyzed. RESULTS: The body weight, liver weight, and epidermal fat content in the HFD group were higher than those of the normal group, and similar to those of the HFD + HT group...
May 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28627061/glycaemic-and-insulinaemic-responses-of-adult-healthy-warm-blooded-mares-following-feeding-with-jerusalem-artichoke-meal
#10
M Glatter, M Bochnia, F Goetz, J Gottschalk, G Koeller, N Mielenz, D Hillegeist, J M Greef, A Einspanier, A Zeyner
This study aimed to investigate the impact of the supplementation of a pre-biotic compound [Jerusalem artichoke meal (JAM)] on the glycaemic and insulinaemic response in healthy, non-obese warm-blooded horses. Six adult mares [mean body weight (bwt) 529 ± 38.7 kg; body condition score 5.1 ± 0.49/9] were used. In two equal meals per day, the horses received crushed oat grains (1 g starch/kg bwt per day) and meadow hay (2 kg/100 kg bwt per day) which together were likely to meet the energy recommendation for light work (GfE, )...
June 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28624998/efficient-conversion-of-fructose-based-biomass-into-lipids-with-trichosporon-fermentans-under-phosphate-limited-conditions
#11
Ruiqi Bao, Xiangying Wu, Sasa Liu, Tongzhou Xie, Chenxu Yu, Xinping Lin
Limiting nitrogen supply has been routinely used as the master regulator to direct lipid biosynthesis. However, this strategy does not work with nitrogen-rich substrates, such as Jerusalem artichoke (JA), a fructose-based biomass, while it is difficult to obtain a high carbon-to-nitrogen (C/N) molar ratio. In this study, an alternative strategy to promote lipid accumulation by the oleaginous yeast Trichosporon fermentans CICC 1368 was developed by limiting phosphorous supply, and this strategy was implemented with JA hydrolysate as substrate...
June 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28616072/the-advanced-strategy-for-enhancing-biobutanol-production-and-high-efficient-product-recovery-with-reduced-wastewater-generation
#12
Chuang Xue, Xiaotong Zhang, Jufang Wang, Min Xiao, Lijie Chen, Fengwu Bai
BACKGROUND: Butanol as an important chemical and potential fuel could be produced via ABE fermentation from lignocellulosic biomass. The use of food-related feedstocks such as maize and sugar cane may not be a sustainable solution to world's energy needs. Recently, Jerusalem artichoke tubers containing inulin have been used as feedstock for butanol production, but this bioprocess is not commercially feasible due to the great value of inulin as functional food. Till now, there is a gap on the utilization of Jerusalem artichoke stalk (JAS) as feedstock for microbial butanol production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28554523/improved-ethanol-production-at-high-temperature-by-consolidated-bioprocessing-using-saccharomyces-cerevisiae-strain-engineered-with-artificial-zinc-finger-protein
#13
M Mahfuza Khatun, Xinshui Yu, Akihiko Kondo, Fengwu Bai, Xinqing Zhao
In this work, the consolidated bioprocessing (CBP) yeast Saccharomyces cerevisiae MNII/cocδBEC3 was transformed by an artificial zinc finger protein (AZFP) library to improve its thermal tolerance, and the strain MNII-AZFP with superior growth at 42°C was selected. Improved degradation of acid swollen cellulose by 45.9% led to an increase in ethanol production, when compared to the control strain. Moreover, the fermentation of Jerusalem artichoke stalk (JAS) by MNII-AZFP was shortened by 12h at 42°C with a concomitant improvement in ethanol production...
May 18, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28521229/development-of-jerusalem-artichoke-resource-for-efficient-one-step-fermentation-of-poly-%C3%AE-glutamic-acid-using-a-novel-strain-bacillus-amyloliquefaciens-nx-2s
#14
Yibin Qiu, Yuanyuan Sha, Yatao Zhang, Zongqi Xu, Sha Li, Peng Lei, Zheng Xu, Xiaohai Feng, Hong Xu
This study aimed to develop non-food fermentation for the cost-effective production of poly-(γ-glutamic acid) (γ-PGA) using a novel strain of Bacillus amyloliquefaciens NX-2S. The new isolate assimilated inulin more efficiently than other carbohydrates from Jerusalem artichoke, without hydrolytic treatment. To investigate the effect of inulin on γ-PGA production, the transcript levels of γ-PGA synthetase genes (pgsB, pgsC, pgsA), regulatory genes (comA, degQ, degS), and the glutamic acid biosynthesis gene (glnA) were analyzed; inulin addition upregulated these key genes...
September 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28341907/utilization-of-inulin-containing-waste-in-industrial-fermentations-to-produce-biofuels-and-bio-based-chemicals
#15
REVIEW
Stephen R Hughes, Nasib Qureshi, Juan Carlos López-Núñez, Marjorie A Jones, Joshua M Jarodsky, Luz Ángela Galindo-Leva, Mitchell R Lindquist
Inulins are polysaccharides that belong to an important class of carbohydrates known as fructans and are used by many plants as a means of storing energy. Inulins contain 20 to several thousand fructose units joined by β-2,1 glycosidic bonds, typically with a terminal glucose unit. Plants with high concentrations of inulin include: agave, asparagus, coffee, chicory, dahlia, dandelion, garlic, globe artichoke, Jerusalem artichoke, jicama, onion, wild yam, and yacón. To utilize inulin as its carbon and energy source directly, a microorganism requires an extracellular inulinase to hydrolyze the glycosidic bonds to release fermentable monosaccharides...
April 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28338054/identification-of-hexose-kinase-genes-in-kluyveromyces-marxianus-and-thermo-tolerant-one-step-producing-glucose-free-fructose-strain-construction
#16
Guorong Zhang, Min Lu, Jichao Wang, Dongmei Wang, Xiaolian Gao, Jiong Hong
In yeast, the hexose assimilation is started at hexose phosphorylation. However, in Kluyveromyces marxianus, the hexokinase (HXK) and glucokinase (GLK) genes were not identified by experiments. Meanwhile, the glucose-free fructose product requires more cost-efficient method. In this study, the KmHXK1 and KmGLK1 genes were functionally identified through gene disruption, over-expression and recombinant enzymes characterization. Both glucose and fructose assimilation ability decreased significantly in KmHXK1 disrupted strain YLM001, however, this ability was not changed obviously in KmGLK1 disrupted strain YLM002...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28315963/enhancement-of-2-3-butanediol-production-from-jerusalem-artichoke-tuber-extract-by-a-recombinant-bacillus-sp-strain-brc1-with-increased-inulinase-activity
#17
Jang Min Park, Baek-Rock Oh, In Yeong Kang, Sun-Yeon Heo, Jeong-Woo Seo, Seung-Moon Park, Won-Kyung Hong, Chul Ho Kim
A Bacillus sp. strain named BRC1 is capable of producing 2,3-butanediol (2,3-BD) using hydrolysates of the Jerusalem artichoke tuber (JAT), a rich source of the fructose polymer inulin. To enhance 2,3-BD production, we undertook an extensive analysis of the Bacillus sp. BRC1 genome, identifying a putative gene (sacC) encoding a fructan hydrolysis enzyme and characterizing the activity of the resulting recombinant protein expressed in and purified from Escherichia coli. Introduction of the sacC gene into Bacillus sp...
July 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28298683/fructan-fructan-1-fructosyltransferase-and-inulin-hydrolase-activities-relating-to-inulin-and-soluble-sugars-in-jerusalem-artichoke-helianthus-tuberosus-linn-tubers-during-storage
#18
Sukanya Maicaurkaew, Sanun Jogloy, Bruce R Hamaker, Suwayd Ningsanond
Influences of harvest time and storage conditions on activities of fructan:fructan1-fructosyltransferase (1-FFT) and inulin hydrolase (InH) in relation to inulin and soluble sugars of Jerusalem artichoke (Helianthus tuberosus L.) tubers were investigated. Maturity affected 1-FFT-activity, inulin contents, and inulin profiles of the tubers harvested between 30 and 70 days after flowering (DAF). Decreases in 1-FFT activity, high molecular weight inulin, and inulin content were observed in late-harvested tubers...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28216099/the-draft-genome-sequence-of-the-ascomycete-fungus-penicillium-subrubescens-reveals-a-highly-enriched-content-of-plant-biomass-related-cazymes-compared-to-related-fungi
#19
Mao Peng, Adiphol Dilokpimol, Miia R Mäkelä, Kristiina Hildén, Sander Bervoets, Robert Riley, Igor V Grigoriev, Matthieu Hainaut, Bernard Henrissat, Ronald P de Vries, Zoraide Granchi
Here we report the genome sequence of the ascomycete saprobic fungus Penicillium subrubescens FBCC1632/CBS132785 isolated from a Jerusalem artichoke field in Finland. The 39.75Mb genome containing 14,188 gene models is highly similar for that reported for other Penicillium species, but contains a significantly higher number of putative carbohydrate active enzyme (CAZyme) encoding genes.
March 20, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28177802/plant-growth-promotion-properties-of-bacterial-strains-isolated-from-the-rhizosphere-of-the-jerusalem-artichoke-helianthus-tuberosus-l-adapted-to-saline-alkaline-soils-and-their-effect-on-wheat-growth
#20
Xiaolin Liu, Xiangyue Li, Yan Li, Runzhi Li, Zhihong Xie
The Jerusalem artichoke (JA; Helianthus tuberosus), known to be tolerant to saline-alkaline soil conditions, has been cultivated for many years in the Yellow River delta, Shandong Province coastal zone, in China. The aim of our study was to isolate nitrogen-fixing bacteria colonizing the rhizosphere of JA and to characterize other plant growth promotion properties. The ultimate goal was to identify isolates that could be used as inoculants benefiting an economic crop, in particular for improving wheat growth production in the Yellow River delta...
March 2017: Canadian Journal of Microbiology
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