keyword
MENU ▼
Read by QxMD icon Read
search

RT-PCR

keyword
https://www.readbyqxmd.com/read/29166725/-effect-and-related-mechanism-of-mirna-21-on-hydrogen-peroxide-induced-c-kit-cardiac-stem-cells-apoptosis
#1
R Z Zhao, Y Wang, W W Deng, B Shi, X P Long, Z L Wang, W M Chen
Objective: To explored the effect and related mechanism of miRNA-21 on hydrogen peroxide-induced C-kit(+) cardiac stem cells apoptosis. Methods: C-kit(+) cardiac stem cells were isolated from SD rats by the methods of enzyme digestion and magnetic bead. Cells were divided into the following experimental groups: (1) negative control mimics (NCM)group: cells were transfected with negative control miRNA-21 mimics for 48 hours; (2)mimics group: cells were transfected with miRNA-21 mimics for 48 hours; (3) NCM+ H(2)O(2) group: negative control miRNA-21 mimics were transfected into cells for 48 hours and then treated with 100 μmol H(2)O(2) for 2 hours; (4)mimics+ H(2)O(2) group: miRNA-21 mimics were transfected into cells for 48 hours and then treated with 100 μmol H(2)O(2) for 2 hours...
November 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29166644/cylindromatosis-cyld-a-deubiquitinase-attenuates-inflammatory-signaling-pathways-by-activating-toll-like-receptor-3-in-human-mesangial-cells
#2
Tadaatsu Imaizumi, Ryo Hayakari, Shojiro Watanabe, Tomomi Aizawa, Tomoh Matsumiya, Hidemi Yoshida, Kazushi Tsuruga, Shogo Kawaguchi, Hiroshi Tanaka
BACKGROUND/AIMS: Cylindromatosis (CYLD), a deubiquitinase, negatively regulates nuclear factor-κB in various cells. However, its potential roles in glomerular inflammation remain unclear. Because the activation of the Toll-like receptor 3 (TLR3)/type I interferon (IFN) pathways plays a pivotal role in chronic kidney diseases (CKD), we examined the role of CYLD in the TLR3 signaling in cultured human mesangial cells (MCs). METHODS: We stimulated CYLD-silenced MCs with polyinosinic-polycytidylic acid (poly IC), a synthetic analogue of dsRNA, and studied representative TLR3/IFN-β pathways (i...
November 22, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29166265/selection-of-reference-genes-for-quantitative-real-time-rt-pcr-on-gene-expression-in-golden-pompano-trachinotus-ovatus
#3
X J Chen, X Q Zhang, S Huang, Z J Cao, Q W Qin, W T Hu, Y Sun, Y C Zhou
Golden pompano (Trachinotus ovatus) is an important economically fish species. In this study, with an aim to identify reliable reference genes for quantitative real-time PCR (qRT-PCR) in golden pompano, we evaluated the expression stability of eight housekeeping genes in the presence and absence of poly I:C stimulation in eight tissues. The PCR data was analyzed by geNorm and NormFinder algorithms. The results showed that the expression of all the examined genes exhibited tissue-dependent variations. When under normal physiological condition, geNorm and NormFinder identified B2M and 18S as suitable genes...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29165998/characterization-of-natural-organic-substances-potentially-hindering-rt-pcr-based-virus-detection-in-large-volumes-of-environmental-water
#4
Akihiko Hata, Manami Inaba, Hiroyuki Katayama, Hiroaki Furumai
Quantitative detection of pathogenic viruses in the environmental water is essential for the assessment of water safety. It is known that some of natural organic substances interfere with virus detection processes, i.e., nucleic acid extraction and reverse transcription-PCR. Such substances are carried over into a sample after virus concentration. In this study, inhibitory substances in coastal water samples were characterized in view of their effects on efficiency of virus detection and property as organic matters...
November 22, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29165657/rotavirus-vaccination-can-be-performed-without-viral-dissemination-in-the-neonatal-intensive-care-unit
#5
Hiroyuki Hiramatsu, Ryota Suzuki, Arisa Nagatani, Hiroko Boda, Masafumi Miyata, Fumihiko Hattori, Hiroki Miura, Ken Sugata, Shigeki Yamada, Satoshi Komoto, Koki Taniguchi, Masaru Ihira, Naoko Nishimura, Takao Ozaki, Tetsushi Yoshikawa
Background: This study was conducted to assess transmissibility of the vaccine strains after RV vaccination in a neonatal intensive care unit (NICU). Methods: Rotateq (RV5) or Rotarix (RV1) was administered to infants admitted to the NICU. Vaccinated infants (VIs, n=19) and unvaccinated infants (UVIs, n=49) whose beds were located in close proximity to the VIs were enrolled in this study. Fecal shedding of vaccine viruses and spreading of the vaccine strains within the NICU were examined using real-time reverse transcriptase polymerase chain reaction (RT-PCR)...
November 20, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29165231/sequence-optimized-real-time-reverse-transcription-polymerase-chain-reaction-assay-for-detection-of-crimean-congo-hemorrhagic-fever-virus
#6
Jeffrey W Koehler, Korey L Delp, Adrienne T Hall, Scott P Olschner, Brian J Kearney, Aura R Garrison, Louis A Altamura, Cynthia A Rossi, Timothy D Minogue
Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne virus of the genus Nairovirus within the family Bunyaviridae. Infection can result in general myalgia, fever, and headache with some patients developing hemorrhagic fever with mortality rates ranging from 5% to 30%. CCHFV has a wide geographic range that includes Africa, Asia, the Middle East, and Europe with nucleotide sequence variation approaching 20% across the three negative-sense RNA genome segments. While phylogenetic clustering generally aligns with geographic origin of individual strains, distribution can be wide due to tick/CCHFV dispersion via migrating birds...
November 20, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29164589/mir-214-protects-mc3t3-e1-osteoblasts-against-h2o2-induced-apoptosis-by-suppressing-oxidative-stress-and-targeting-atf4
#7
X-Z Lu, Z-H Yang, H-J Zhang, L-L Zhu, X-L Mao, Y Yuan
OBJECTIVE: Fragility fracture is one of the common complications of osteoporosis. Elevated oxidative stress-induced apoptosis is thought to be one of the unfavorable factors to osteoblastic dysfunction, which increased the risk of bone fracture. However, the molecular mechanisms for oxidative stress-induced osteoblast cells apoptosis still need to be elucidated. This study aims to investigate the protective function of miR-214 in H2O2-induced apoptosis of MC3T3-E1 osteoblasts. MATERIALS AND METHODS: MC3T3-E1 cells were treated with 400 μM H2O2...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29164574/mechanism-of-as2o3-induces-apoptosis-of-glioma-u87-cells
#8
G-B Wang, J-H Liu, J Hu, K Xue
OBJECTIVE: To investigate the mechanism of arsenic trioxide (As2O3)-induced apoptosis of glioma cells. MATERIALS AND METHODS: U87 cells were treated by different concentrations of As2O3 (8 μmol/L, 6 μmol/L, 4 μmol/L, 2 μmol/L, 1 μmol/L and 0.5 μmol/L) for 24 h, 48 h and 72 h, respectively. Cell viability was detected by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the appropriate dosage and time were screened. Transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL) was used to stain cells, followed by an investigation on the apoptosis of cells...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29164560/the-expression-of-transforming-growth-factor-%C3%AE-1-in-myocardial-tissue-and-concentration-of-serum-b-type-natriuretic-peptide-in-myocardial-remodeling-of-sprague-dawley-rats-treated-with-carvedilol
#9
R Yi, G Xiangyu, S Min
OBJECTIVE: The objective of this study was to observe the expression of transforming growth factor-β1 (TGF-β1) in myocardial tissue and the concentration of serum B-type natriuretic peptide (BNP) in myocardial remodeling of Sprague-Dawley rats induced by isoproterenol (ISO) and the effects of carvedilol intervention. MATERIALS AND METHODS: Thirty rats were divided randomly into three groups: (1) Control group: rats were injected with 5 mL/(kg·d) of saline for 10 days, followed by 10 mL/(kg·d) of saline by gavage for 4 weeks...
November 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29164460/di-2-ethylhexyl-phthalate-induces-glucose-metabolic-disorder-in-adolescent-rats
#10
Jin Xu, Liting Zhou, Shuyue Wang, Jian Zhu, Te Liu, Yiyang Jia, Di Sun, Huaiji Chen, Qi Wang, Feng Xu, Yuezhu Zhang, Hongbo Liu, Tianrong Zhang, Lin Ye
As a plasticizer, di-(2-ethylhexyl)-phthalate (DEHP) is widely added in various commercial products. Some researchers had suggested that DEHP has adverse effects on the glucose metabolism, but the mechanisms remain unclear. Adolescent Wistar rats were divided into four groups and administered DEHP by gavage at 0, 5, 50, and 500 mg kg(-1) d(-1) for 28 days. ELISA was used to quantify the serum insulin and leptin levels; RT-PCR, immunohistochemistry, and Western blot were used to detect the mRNA and protein expressions of Janus-activated kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3), suppressor of cytokine signaling 3 (SOCS3), leptin receptor (Ob-R), and insulin receptor (IR) in liver and pancreas In comparison to the control group, the DEHP-treated rats showed the following: (1) higher organ coefficient of liver; (2) higher fasting blood glucose levels, higher fasting serum insulin and leptin levels, higher insulin resistance index homeostasis model assessment; (3) lower protein levels of Ob-R and IR in the liver and pancreas; (4) higher protein levels of JAK2 and STAT3 in the liver; and (5) higher protein and mRNA levels of SOCS3 in the liver and pancreas...
November 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29164336/stimulation-of-chemokines-in-human-endometrial-stromal-cells-by-tumor-necrosis-factor-%C3%AE-and-interferon-%C3%AE-is-similar-under-apoptotic-and-non-apoptotic-conditions
#11
Julia Spratte, Henriette Princk, Florian Schütz, Joachim Rom, Marek Zygmunt, Herbert Fluhr
PURPOSE: Tumor necrosis factor (TNF)-α and interferon (IFN)-γ are pro-inflammatory cytokines which have been shown to be involved in the pathophysiology of implantation disorders. Both cytokines in combination are able to sensitize primarily resistant human endometrial stromal cells (ESCs) to Fas-induced apoptosis. Since CCL (CC-chemokine ligand) 5 and CCL2 are important regulators of the endometrial immune cell population, we examined the impact of TNF-α and IFN-γ on these two chemokines under non-apoptotic and apoptotic conditions...
November 21, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29164313/genome-wide-analysis-of-a-recently-active-retrotransposon-au-sine-in-wheat-content-distribution-within-subgenomes-and-chromosomes-and-gene-associations
#12
Danielle Keidar, Chen Doron, Khalil Kashkush
Here, we show that Au SINE elements have strong associations with protein-coding genes in wheat. Most importantly Au SINE insertion within introns causes allelic variation and might induce intron retention. The impact of transposable elements (TEs) on genome structure and function is intensively studied in eukaryotes, especially in plants where TEs can reach up to 90% of the genome in some cases, such as in wheat. Here, we have performed a genome-wide in-silico analysis using the updated publicly available genome draft of bread wheat (T...
November 21, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/29164291/bacterial-diversity-in-traditional-doogh-in-comparison-to-industrial-doogh
#13
Hamid Reza Sayevand, Farzaneh Bakhtiary, Angelika Pointner, Marlene Remely, Berit Hippe, Hedayat Hosseini, Alexander Haslberger
Forty-four samples of traditional Doogh and yoghurt were collected from 13 regions of 4 provinces in west of Iran (13 area) and analyzed using molecular methods including PCR, denaturing gradient gel electrophoresis (DGGE) of 16S rDNA, and sequencing. Moreover, collected samples as well as samples from industrially Doogh were analyzed with quantitative real-time PCR (RT-PCR). Analyzed 16S rRNA gene sequences of Doogh samples could be allocated to the presence of Lactobacillus spp. The typical yoghurt starter culture bacteria included four different Lactobacillus species with possible probiotic properties, L...
November 21, 2017: Current Microbiology
https://www.readbyqxmd.com/read/29163988/effect-of-mt3-on-retinal-and-choroidal-tgf-%C3%AE-2-and-has2-expressions-in-form-deprivation-myopia-of-guinea-pig
#14
Tao Li, Xiaodong Zhou, Bing Li, Bo Jiang
Purpose: To confirm its dose-dependent effect on form deprivation myopia and evaluate the effect of MT3 at different tissue concentrations on changes in mRNA and protein expression for TGF-β2 and HAS2. Methods: MT3 was intravitreally injected into deprived eyes at two-day intervals. Refraction was measured by streak retinoscopy after cycloplegia. The axial dimensions were measured by A-scan ultrasound. The quantitative RT-PCR and Western blot were used to detect the changes of TGF-β2 and HAS2 expressions in the retina and choroid of guinea pigs...
2017: Journal of Ophthalmology
https://www.readbyqxmd.com/read/29163675/effects-of-different-concentrations-of-type-i-collagen-hydrogel-on-the-growth-and-differentiation-of-chondrocytes
#15
Deshan Hu, Xiuli Shan
The objective of this study was to analyze the effects of type-I collagen hydrogel of different concentrations on the growth and differentiation of rabbit chondrocytes. Articular cartilage from New Zealand white rabbits was harvested and cultured. Second-generation chondrocytes were collected for in vitro culture with 10, 7, and 5 mg/ml type-I collagen hydrogel, respectively (denoted as groups A, B, and C). After in vitro culture for 1 day, chondrocytes were stained with fluorescein diacetate (FDA)/propidium iodide (PI), and cell viability was observed by laser confocal microscopy...
December 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29163643/persistent-bovine-viral-diarrhea-virus-bvdv-infection-in-cattle-herds
#16
REVIEW
A Khodakaram-Tafti, G H Farjanikish
Bovine viral diarrhea virus (BVDV) is a significant pathogen associated with gastrointestinal, respiratory, and reproductive diseases of cattle worldwide. It causes continuous economic losses to the cattle industry primarily due to decreased reproductive performance. The ability of virus to cross the placenta during early pregnancy can result in the birth of persistently infected (PI) calves. Persistently infected animals are generally much more efficient transmitters of BVDV than transiently or acutely infected animals because they are capable of shedding large quantities of virus throughout their lives and are considered the primary reservoirs for BVDV...
2017: Iranian journal of veterinary research
https://www.readbyqxmd.com/read/29163484/tracking-human-immunodeficiency-virus-1-infection-in-the-humanized-drag-mouse-model
#17
Jiae Kim, Kristina K Peachman, Ousman Jobe, Elaine B Morrison, Atef Allam, Linda Jagodzinski, Sofia A Casares, Mangala Rao
Humanized mice are emerging as an alternative model system to well-established non-human primate (NHP) models for studying human immunodeficiency virus (HIV)-1 biology and pathogenesis. Although both NHP and humanized mice have their own strengths and could never truly reflect the complex human immune system and biology, there are several advantages of using the humanized mice in terms of using primary HIV-1 for infection instead of simian immunodeficiency virus or chimera simian/HIV. Several different types of humanized mice have been developed with varying levels of reconstitution of human CD45(+) cells...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29163458/molecular-biology-can-change-the-classic-laboratory-approach-for-intestinal-protozoan-infections
#18
Fabio Formenti, Matteo Valerio, Massimo Guerriero, Francesca Perandin, Barbara Pajola, Manuela Mistretta, Stefano Tais, Monica Degani, Zeno Bisoffi
For many years microscopy has been considered the mainstay of the diagnosis of parasitic infections. In our laboratory, before the advent of molecular biology, the approach for the identification of parasitic infections in stools was the microscopic exam of three samples. Once we adopted molecular biology, a real-time PCR on one single sample was added to the classical coproparasitological exam of three samples. Given the high sensitivity of real-time PCR (Rt-PCR), we then decided to evaluate if a change of our routine was justified...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29162986/hyperoside-attenuates-dextran-sulfate-sodium-induced-colitis-in-mice-possibly-via-activation-of-the-nrf2-signalling-pathway
#19
Lei Yang, Lei Shen, Yue Li, Yanxia Li, Shijie Yu, Shanshan Wang
Background: Hyperoside (Hyp) is a flavonoid glycoside compound that has been demonstrated to have anti-inflammatory, anti-apoptotic and antioxidant effects. However, the impact of Hyp on inflammatory bowel disease (IBD) has not been previously explored. Thus, we evaluated the role of Hyp in dextran sodium sulfate (DSS)-induced acute colitis in mice. Methods: We established a mouse model of experimental acute colitis by treating mice with drinking water supplemented with 3...
2017: Journal of Inflammation
https://www.readbyqxmd.com/read/29162985/cbfa2t2-is-associated-with-a-cancer-stem-cell-state-in-renal-cell-carcinoma
#20
Du-Chu Chen, You-De Liang, Liang Peng, Yi-Ze Wang, Chun-Zhi Ai, Xin-Xing Zhu, Ya-Wei Yan, Yasmeen Saeed, Bin Yu, Jingying Huang, Yuxin Gao, Jiaqi Liu, Yi-Zhou Jiang, Min Liu, Demeng Chen
Background: Renal cell carcinoma (RCC) is the most common kidney cancer, accounting for approximately 80-90% of all primary kidney cancer. Treatment for patients with advanced RCC remains unsatisfactory. Rare cancer stem cells (CSCs) are proposed to be responsible for failure of current treatment. Methods: OncoLnc was used as a tool for interactively exploring survival correlations. Gene manipulation and expression analysis were carried out using siRNA, RT-PCR and Western blotting...
2017: Cancer Cell International
keyword
keyword
97095
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"