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https://www.readbyqxmd.com/read/29334709/-molecular-epidemiological-study-of-human-coronavirus-oc43-in-shanghai-from-2009-2016
#1
Y J Yang, Y W Hu
Objective: To understand the epidemiological characteristics of Human coronavirus (HCoV), the patterns of emergence and circulation, and the genotype distribution of human coronavirus OC43 (HCoV-OC43) from November, 2009 to April, 2016 in Shanghai. Methods: A total of 6 059 respiratory specimens, including pharyngeal swab, sputum, nasopharyngeal aspirates and alveolar lavage fluid, as well as relative clinical data were collected from patients with acute respiratory infections from seven sentinel hospitals during November, 2009 to April, 2016 in Shanghai...
January 6, 2018: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29334668/lidanpaidu-prescription-alleviates-lipopolysaccharide-induced-acute-kidney-injury-by-suppressing-the-nf-%C3%AE%C2%BAb-signaling-pathway
#2
Fan Zhang, Shan Lu, Siyi Jin, Keli Chen, Juan Li, Bisheng Huang, Yan Cao
The Lidanpaidu Prescription (LDP), a hospital preparation, composed of Chinese classical preparations, has been reported to have antiendotoxin, anticoagulant and other effects. However, its therapeutic effect on lipopolysaccharide (LPS)-induced acute kidney injury (AKI) and the mechanisms remain unclear. Therefore, we administered LPD pretreatment at different doses to examine the protective effects and mechanisms in LPS-induced AKI in mice. The kidney injury induced by LPS was assessed by histological examination...
January 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29334656/activated-protein-c-suppresses-osteoclast-differentiation-via-endothelial-protein-c-receptor-protease-activated-receptor-1-sphingosine-1-phosphate-receptor-and-apolipoprotein-e-receptor-2
#3
Kakunoshin Yoshida, Nobuyuki Akita, Takayuki Okamoto, Kunihiro Asanuma, Atsumasa Uchida, Akihiro Sudo, Motomu Shimaoka, Koji Suzuki, Tatsuya Hayashi
INTRODUCTION: Bone remodeling relies on a delicate balance between formation and resorption of bone tissues, processes in which bone-forming osteoblasts and bone-resorbing osteoclasts play central roles. Recently, we reported that anticoagulant activated protein C (APC) promotes osteoblast proliferation, but the role of the blood coagulation system in bone remodeling remains unclear. In this study, to further elucidate the relationship between bone remodeling and blood coagulation, we investigated the effect of APC on osteoclast differentiation...
January 3, 2018: Thrombosis Research
https://www.readbyqxmd.com/read/29334295/neuritin-provides-neuroprotection-against-experimental-traumatic-brain-injury-in-rats
#4
Qi Liu, Hang Zhang, Jian Xu, Dong Zhao
Objectives Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. Neuritin is a neurotrophic factor that regulates neural growth and development. However, the role of neuritin in alleviating TBI has not been investigated. Methods In this study, Sprague Dawley rats (n = 144) weighing 300 ± 50 g were categorized into control, sham, TBI, and TBI + neuritin groups. The neurological scores and the ultrastructure of cortical neurons, apoptotic cells, and caspase-3 were measured by using Garcia scoring system, transmission electron microscopy, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, Western blot analysis, and real-time RT-PCR at various time points post-TBI...
January 15, 2018: International Journal of Neuroscience
https://www.readbyqxmd.com/read/29334172/silica-nanoparticle-induced-cytokine-responses-in-beas-2b-and-hbec3-kt-cells-significance-of-particle-size-and-signalling-pathways-in-different-lung-cell-cultures
#5
Marit Låg, Tonje Skuland, Anna Godymchuk, Thu H T Nguyen, Hang L T Pham, Magne Refsnes
We have previously reported that silica nanoparticles (SiNPs) of nominal size 50 nm (Si50) induce the pro-inflammatory cytokines CXCL8 and IL-6 in BEAS-2B cells, via mechanisms involving MAPK p38, TACE-mediated TGFα release and the NFκB pathway. In the present study, we examined whether these findings are cell-specific or might be extended to another epithelial lung cell model, HBEC3-KT, and also to SiNPs of a smaller size (nominal size of 10 nm; Si10). The TEM average size of Si10 and Si50 was 10.9 nm and 34...
January 15, 2018: Basic & Clinical Pharmacology & Toxicology
https://www.readbyqxmd.com/read/29333916/cytoplasmic-collagen-xi%C3%AE-i-as-a-prognostic-biomarker-in-esophageal-squamous-cell-carcinoma
#6
Bohan Zhang, Cheng Zhang, Xuetao Yang, Yue Chen, Hongqi Zhang, Jingshu Liu, Qingchen Wu
Stromal/cytoplasmic collagen XIαI (COL11A1) has been highlighted in the process of neoplastic transformation, including epithelial mensenchymal transition (EMT), metastasis and invasiveness. In this study, we aim to illuminate the clinical significance and biological role of COL11A1 in esophageal squamous cell carcinoma (ESCC). Herein, we investigated COL11A1 expression in 16 pairs of ESCC and adjacent normal tissues by RT-PCR and western blotting analysis. Correlations of COL11A1 expression with clinicopathologic parameters and survival status were then determined by immunohistochemistry in 116 ESCC and 50 normal specimens...
January 15, 2018: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29333720/degree-of-sglt1-phosphorylation-is-associated-with-but-does-not-determine-segment-specific-glucose-transport-features-in-the-porcine-small-intestines
#7
Stefanie Klinger, Patrick Lange, Elisabeth Brandt, Karin Hustedt, Bernd Schröder, Gerhard Breves, Jens Herrmann
Glucose-induced electrogenic ion transport is higher in the porcine ileum compared with the jejunum despite equal apical abundance of SGLT1. The objective of this study was a detailed determination of SGLT1 and GLUT2 expressions at mRNA and protein levels along the porcine small intestinal axis. Phosphorylation of SGLT1 at serine 418 was assessed as a potential modulator of activity. Porcine intestinal tissues taken along the intestinal axis 1 h or 3 h after feeding were analyzed for relative mRNA (RT-PCR) and protein levels (immunoblot) of SGLT1, pSGLT1, GLUT2, (p)AMPK, β2 -receptor, and PKA substrates...
January 2018: Physiological Reports
https://www.readbyqxmd.com/read/29333035/the-activity-of-immunoglobulin-y-anti-mycobacterium-tuberculosis-on-proliferation-and-cytokine-expression-of-rat-peripheral-blood-mononuclear-cells
#8
Sri Agus Sudjarwo, Koerniasari Eraiko, Giftania Wardani Sudjarwo, Koerniasari
Objective: It has long been known that chickens, like mammals, are capable of producing antigen-specific immunoglobulin Y (IgY), which functions similar to IgG. The present study was performed to investigate the activity of IgY anti-Mycobacterium tuberculosis on proliferation, interleukin (IL)-2, and interferon (IFN)-γ expression of rat peripheral blood mononuclear cells (PBMCs). Materials and Methods: The activity of IgY anti-M. tuberculosis in different doses (25, 50, and 100 μg/ml) on rat PBMCs proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay...
December 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/29332592/construction-of-an-m1gs-ribozyme-for-targeted-and-rapid-mrna-cleavage-application-on-the-ets-2-oncogene
#9
Chrisavgi Toumpeki, Dimitrios Anastasakis, Ioannis Panagoulias, Vassiliki Stamatopoulou, Tassos Georgakopoulos, Sofia Kallia-Raftopoulos, Athanasia Mouzaki, Denis Drainas
Background RNase P-mediated cleavage of target RNAs has been proposed as a promising tool for gene silencing. Ets-2 proto-oncogene controls the expression of a wide variety of genes involved in cancer and immunity. Objective Construction of a functional RNase P-based ribozyme (M1GS303) that targets Ets-2 mRNA Method The accessible sites for targeting of Ets-2 mRNA were identified by footprinting analysis. M1GS303 ribozyme was constructed by cloning. The activity of the ribozyme in the presence or absence of spiramysin in E...
January 11, 2018: Medicinal Chemistry
https://www.readbyqxmd.com/read/29331511/maresin-1-regulates-autophagy-and-inflammation-in-human-periodontal-ligament-cells-through-glycogen-synthase-kinase-3%C3%AE-%C3%AE-catenin-pathway-under-inflammatory-conditions
#10
Li Du, Yucheng Li, Weifeng Liu
OBJECTIVE: Accumulating lines of evidence suggest that maresin 1 (MaR-1) exerts anti-inflammatory effects in many cell types and plays beneficial roles in inflammatory disease, such as peritonitis and colitis. Moreover, it has been demonstrated that MaR-1 play protective roles against localized aggressive periodontitis. However, the function and mechanism of MaR-1 in human periodontal ligament cells (PDL) cells from periodontitis are poorly understood. The present study aimed to clarify the effects and molecular mechanism of MaR-1 in PDL cell survival and inflammation...
December 23, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29331388/e4bp4-inhibits-angii-induced-apoptosis-in-h9c2-cardiomyoblasts-by-activating-the-pi3k-akt-pathway-and-promoting-calcium-uptake
#11
Bih-Cheng Chen, Marthandam Asokan Shibu, Chia-Hua Kuo, Chia-Yao Shen, Shu Nu Chang-Lee, Chao-Hung Lai, Ray-Jade Chen, Chun-Hsu Yao, Vijaya Padma Viswanadha, Jian-Shen Liu, Wei-Kung Chen, Chih-Yang Huang
The bZIP transcription factor E4BP4 is a survival factor that is known to be elevated in diseased heart and promote cell survival. In this study the role of E4BP4 on angiotensin-II (AngII)-induced apoptosis has been examined in in vitro cell model. H9c2 cardiomyoblast cells that overexpressed E4BP4 were exposed to AngII to observe the cardio-protective effects of E4BP4 on hypertension related apoptosis. The results from TUNEL assays revealed that E4BP4 significantly attenuated AngII-induced apoptosis. Further analysis by Western blot and RT-PCR showed that E4BP4 inhibited AngII-induced IGF-II mRNA expression and cleavage of caspase-3 through the PI3K-Akt pathway...
January 10, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29330702/ptac10-an-s1-domain-containing-component-of-the-transcriptionally-active-chromosome-complex-is-essential-for-plastid-gene-expression-in-arabidopsis-thaliana-and-is-phosphorylated-by-chloroplast-targeted-casein-kinase-ii
#12
Qing-Bo Yu, Tuan-Tuan Zhao, Lin-Shan Ye, Ling Cheng, Ying-Qian Wu, Chao Huang, Zhong-Nan Yang
In higher plant chloroplasts, the plastid-encoded RNA polymerase (PEP) consists of four catalytic subunits and numerous nuclear-encoded accessory proteins, including pTAC10, an S1-domain-containing protein. In this study, pTAC10 knockout lines were characterized. Two ptac10 mutants had an albino phenotype and severely impaired chloroplast development. The pTAC10 genomic sequence fused to a four-tandem MYC tag driven by its own promoter functionally complemented the ptac10-1 mutant phenotype. pTAC10 was present in both the chloroplast stroma and thylakoids...
January 12, 2018: Photosynthesis Research
https://www.readbyqxmd.com/read/29330614/effect-of-ancymidol-on-cell-wall-metabolism-in-growing-maize-cells
#13
J Mabel Hernández-Altamirano, Asier Largo-Gosens, Romina Martínez-Rubio, Diego Pereda, Jesús M Álvarez, José L Acebes, Antonio Encina, Penélope García-Angulo
Ancymidol inhibits the incorporation of cellulose into cell walls of maize cell cultures in a gibberellin-independent manner, impairing cell growth; the reduction in the cellulose content is compensated with xylans. Ancymidol is a plant growth retardant which impairs gibberellin biosynthesis. It has been reported to inhibit cellulose synthesis by tobacco cells, based on its cell-malforming effects. To ascertain the putative role of ancymidol as a cellulose biosynthesis inhibitor, we conducted a biochemical study of its effect on cell growth and cell wall metabolism in maize cultured cells...
January 12, 2018: Planta
https://www.readbyqxmd.com/read/29330370/a-rna-sequencing-approach-for-the-identification-of-novel-long-non-coding-rna-biomarkers-in-colorectal-cancer
#14
Atsushi Yamada, Pingjian Yu, Wei Lin, Yoshinaga Okugawa, C Richard Boland, Ajay Goel
Long non-coding RNAs (lncRNAs) have been implicated in human pathology, however, their role in colorectal carcinogenesis have not been fully elucidated. In the current study, whole-transcriptome analysis was performed in 3 pairs of colorectal cancer (CRC) and matched normal mucosa (NM) by RNA sequencing (RNA-seq). Followed by confirmation using the Cancer Genome Atlas (TCGA) dataset, we identified 27 up-regulated and 22 down-regulated lncRNAs in CRC. Up-regulation of four lncRNAs, hereby named colorectal cancer associated lncRNA (CRCAL)-1 [AC021218...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330084/real-time-reverse-transcription-recombinase-polymerase-amplification-assay-for-rapid-detection-of-porcine-epidemic-diarrhea-virus
#15
Jianchang Wang, Ruoxi Zhang, Jinfeng Wang, Qingan Han, Libing Liu, Yanan Li, Wanzhe Yuan
Porcine epidemic diarrhea (PED), which is caused by porcine epidemic diarrhea virus (PEDV), is an acute, highly contagious enteric disease characterized by severe watery diarrhea, vomiting, dehydration, and high mortality in suckling piglets. A real-time reverse-transcription recombinase polymerase amplification assay (RT-RPA) was developed based on the nucleocapsid gene of PEDV. RT-RPA assay was performed at 40 °C for 20 min. The assay could detect both the classical and variant PEDV strains, and there was no cross-reaction with other pathogens tested...
January 9, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29330013/a-functional-splicing-variant-associated-with-decreased-asthma-risk-abolishes-the-ability-of-gasdermin-b-gsmdb-to-induce-epithelial-cell-pyroptosis
#16
Ronald A Panganiban, Maoyun Sun, Amber Dahlin, Hae-Ryung Park, Mengyuan Kan, Blanca E Himes, Jennifer A Mitchel, Carlos Iribarren, Eric Jorgenson, Scott H Randell, Elliot Israel, Kelan Tantisira, Stephanie Shore, Jin-Ah Park, Scott T Weiss, Ann Chen Wu, Quan Lu
BACKGROUND: Genetic variants in the chromosomal region 17q21 are consistently associated with asthma. However, mechanistic studies have not yet linked any of the associated variants to a function that could influence asthma, and as a result, the identity of the asthma gene(s) remains elusive. OBJECTIVES: We sought to identify and characterize functional variants in the 17q21 locus. METHODS: We used the Exome Aggregation Consortium (ExAC) browser to identify coding (amino acid-changing) variants in the 17q21 locus...
January 9, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29330010/nasopharyngeal-lactobacillus-is-associated-with-childhood-wheezing-illnesses-following-acute-respiratory-syncytial-virus-infection-in-infancy
#17
Christian Rosas-Salazar, Meghan H Shilts, Andrey Tovchigrechko, Seth Schobel, James D Chappell, Emma K Larkin, Tebeb Gebretsadik, Rebecca A Halpin, Karen E Nelson, Martin L Moore, Larry J Anderson, R Stokes Peebles, Suman R Das, Tina V Hartert
BACKGROUND: Early-life acute respiratory infection (ARI) with respiratory syncytial virus (RSV) has been strongly associated with the development of childhood wheezing illnesses, but the pathways underlying this association are poorly understood. OBJECTIVE: To examine the role of the nasopharyngeal microbiome in the development of childhood wheezing illnesses following RSV ARI in infancy. METHODS: We conducted a nested cohort study of 118 previously healthy, term infants with confirmed RSV ARI by RT-PCR...
January 9, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29329758/real-time-rt-pcr-a-necessary-tool-to-support-the-diagnosis-and-surveillance-of-rotavirus-in-mexico
#18
Sergio Isaac De La Cruz Hernández, Yazmin Anaya Molina, Fabián Gómez Santiago, Heidi Lizbeth Terán Vega, Elda Monroy Leyva, Héctor Méndez Pérez, Herlinda García Lozano
Rotavirus produces diarrhea in children under 5 years old. Most of those conventional methods such as polyacrylamide gel electrophoresis (PAGE) and reverse transcription-polymerase chain reaction (RT-PCR) have been used for rotavirus detection. However, these techniques need a multi-step process to get the results. In comparison with conventional methods, the real-time RT-PCR is a highly sensitive method, which allows getting the results in only one day. In this study a real-time RT-PCR assay was tested using a panel of 440 samples from patients with acute gastroenteritis, and characterized by PAGE and RT-PCR...
December 14, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29329647/atractylodes-lancea-rhizome-water-extract-reduces-triptolide-induced-toxicity-and-enhances-anti-inflammatory-effects
#19
Yuan Wei, Dan-Juan Sui, Hai-Miao Xu, Zhen Ouyang, Na Wu, Du-Jun Wang, Xiao-Yan Zhang, Da-Wei Qian
The present study was designed to explore the influence of water extracts of Atractylodes lancea rhizomes on the toxicity and anti-inflammatory effects of triptolide (TP). A water extract was prepared from A. lancea rhizomes and co-administered with TP in C57BL/6 mice. The toxicity was assayed by determining serum biochemical parameters and visceral indexes and by liver histopathological analysis. The hepatic CYP3A expression levels were detected using Western blotting and RT-PCR methods. The data showed that the water extract of A...
December 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29329610/bacopa-monnieri-extracts-prevent-hydrogen-peroxide-induced-oxidative-damage-in-a-cellular-model-of-neuroblastoma-imr32-cells
#20
Gaurav Bhatia, Vikram Dhuna, Kshitija Dhuna, Manpreet Kaur, Jatinder Singh
Neurodegenerative diseases are the consequences of imbalance between the production of oxidative stress and its nullification by cellular defense mechanisms. Hydrogen peroxide (H2O2), a precursor of deleterious reactive oxygen species, elicits oxidative stress, resulting in severe brain injuries. Bacopa monnieri is well known for its nerve relaxing and memory enhancing properties. The present study was designed to evaluate the protective effects of extracts from Bacopa monnieri against H2O2 induced oxidative stress using a cellular model, neuroblastoma IMR32 cell line...
November 2017: Chinese Journal of Natural Medicines
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