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https://www.readbyqxmd.com/read/28750349/escherichia-coli-maltose-binding-protein-mbp-activates-mouse-th1-through-tlr2-mediated-myd88-dependent-pathway-and-tlr4-mediated-trif-dependent-pathway
#1
GuoMu Liu, YiXin Zhang, NanNan Zhang, WeiHua Ni, Jing Jie, LiNa Jiang, GuiXiang Tai
MBP (maltose-binding protein) is a component of Escherichia coli. Our previous study found that MBP directly induces the activation of Th1 (T helper type 1), but the molecular mechanism remains unclear. In the present study, CD4(+)T cells were purified from the spleens of normal mice using antibody-coated immunomagnetic beads by negative selection. CD4(+)T cells activated with a CD3/CD28 antibody were stimulated with MBP. The results indicated that MBP elevated IFN-γ mRNA levels in activated CD4(+)T cells and promoted IFN-γ production from activated CD4(+)T cells...
July 24, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28750058/human-enteroviruses-associated-with-and-without-diarrhea-in-thailand-between-2010-and-2016
#2
Jira Chansaenroj, Supansa Tuanthap, Thanundorn Thanusuwannasak, Ausanee Duang-In, Sirapa Klinfueng, Napha Thaneskongtong, Viboonsuk Vutithanachot, Sompong Vongpunsawad, Yong Poovorawan
Non-bacterial acute gastroenteritis (AGE) associated with virus infection affects individuals living in developing countries, especially children. To investigate whether shedding of certain human enterovirus (EV) is more frequently detected in the stool of individuals with AGE of unknown etiology than individuals without AGE symptoms, we tested fecal samples collected from 2,692 individuals with diarrhea between January 2010 and December 2016. Samples were tested for rotavirus, norovirus, and EV by reverse-transcription polymerase chain reaction (RT-PCR) and adenovirus by PCR...
2017: PloS One
https://www.readbyqxmd.com/read/28750051/acute-resistance-exercise-modulates-microrna-expression-profiles-combined-tissue-and-circulatory-targeted-analyses
#3
Randall F D'Souza, James F Markworth, Kirsten M M Aasen, Nina Zeng, David Cameron-Smith, Cameron J Mitchell
A subset of short non-coding RNAs, microRNAs (miRs), have been identified in the regulation of skeletal muscle hypertrophy and atrophy. Expressed within cells, miRs are also present in circulation (c-miR) and have a putative role in cross-tissue signalling. The aim of this study was to examine the impact of a single bout of high intensity resistance exercise (RE) on skeletal muscle and circulatory miRs harvested simultaneously. Resistance trained males (n = 9, 24.6 ± 4.9 years) undertook a single bout of high volume RE with venous blood and muscle biopsies collected before, 2 and 4hr post-exercise...
2017: PloS One
https://www.readbyqxmd.com/read/28749991/nanofluidic-digital-pcr-for-the-quantification-of-norovirus-for-water-quality-assessment
#4
Silvia Monteiro, Ricardo Santos
Sensitive detection of water- and foodborne enteric viruses is extremely relevant, especially due to the low concentrations in which they are found. Accurate and sensitive detection of Norovirus, the primary responsible for water- and foodborne outbreaks, is of particular importance. Quantification of Norovirus is commonly performed by quantitative RT-PCR (RT-qPCR). In recent years a new platform was developed, digital PCR, that quantifies without the need for a standard curve thus decreasing the errors associated with its utilization...
2017: PloS One
https://www.readbyqxmd.com/read/28749955/simultaneous-detection-of-three-pome-fruit-tree-viruses-by-one-step-multiplex-quantitative-rt-pcr
#5
Ioanna Malandraki, Despoina Beris, Ioannis Isaioglou, Antonio Olmos, Christina Varveri, Nikon Vassilakos
A one-step multiplex real-time reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan probes was developed for the simultaneous detection of Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in total RNA of pome trees extracted with a CTAB method. The sensitivity of the method was established using in vitro synthesized viral transcripts serially diluted in RNA from healthy, virus-tested (negative) pome trees. The three viruses were simultaneously detected up to a 10-4 dilution of total RNA from a naturally triple-infected apple tree prepared in total RNA of healthy apple tissue...
2017: PloS One
https://www.readbyqxmd.com/read/28749408/clinical-role-of-asct2-slc1a5-in-kras-mutated-colorectal-cancer
#6
Kosuke Toda, Gen Nishikawa, Masayoshi Iwamoto, Yoshiro Itatani, Ryo Takahashi, Yoshiharu Sakai, Kenji Kawada
Mutation in the KRAS gene induces prominent metabolic changes. We have recently reported that KRAS mutations in colorectal cancer (CRC) cause alterations in amino acid metabolism. However, it remains to be investigated which amino acid transporter can be regulated by mutated KRAS in CRC. Here, we performed a screening of amino acid transporters using quantitative reverse-transcription polymerase chain reaction (RT-PCR) and then identified that ASCT2 (SLC1A5) was up-regulated through KRAS signaling. Next, immunohistochemical analysis of 93 primary CRC specimens revealed that there was a significant correlation between KRAS mutational status and ASCT2 expression...
July 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28749283/seroprevalence-and-molecular-characterization-of-ferlavirus-in-captive-vipers-of-costa-rica
#7
Cristina Solis, Randall Arguedas, Mario Baldi, Martha Piche, Carlos Jimenez
Ferlaviruses (FV, previously referred to as ophidian paramyxoviruses, OPMV), are enveloped viruses with a negative-strand RNA genome, affecting snakes in captivity worldwide. Infection is characterized by respiratory and nervous clinical signs and carries high mortality rates, but no specific treatment or vaccine is currently available. Costa Rica has 16 species of vipers, found in captivity in collections essential for antivenom production, reintroduction, and public education. FV circulation in these populations was previously unknown, and the risk of introducing the viruses into naïve collections or free-ranging populations exists if the virus's presence is confirmed...
June 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28749076/serum-and-gene-expression-levels-of-ct-1-il-6-and-tnf-%C3%AE-after-a-lifestyle-intervention-in-obese-children
#8
A Marti, L Morell-Azanza, T Rendo-Urteaga, S García-Calzón, A Ojeda-Rodríguez, N Martín-Calvo, M J Moreno-Aliaga, J A Martínez, M C Azcona-San Julián
BACKGROUND: Inflammation related molecules such as tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and cardiotrophin-1 (CT-1) are highly expressed in obese individuals and could partly explain some comorbidities associated to obesity. In obese children, lifestyle interventions are able to lower inflammation and reduce cardiovascular risk factors associated with obesity. The aim of the present work was to study changes in inflammation-related molecules serum and peripheral blood mononuclear cells (PBMC) transcript levels after a 10-week lifestyle intervention in obese children and asses their potential association with glucose metabolism...
July 27, 2017: Pediatric Diabetes
https://www.readbyqxmd.com/read/28749049/mineral-trioxide-aggregate-enhances-the-osteogenic-capacity-of-periodontal-ligament-stem-cells-via-nf-%C3%AE%C2%BAb-and-mapk-signaling-pathways
#9
Yanqiu Wang, Yixiang Zhou, Lin Jin, Xiyao Pang, Yadie Lu, Zilu Wang, Yan Yu, Jinhua Yu
INTRODUCTION: Mineral trioxide aggregate (MTA), as a bioactive material, has a widespread application in clinical practice. To date, the effects of MTA on the proliferation and differentiation of human periodontal ligament stem cells (hPDLSCs) remain unclear. MATERIALS AND METHODS: hPDLSCs were isolated from human periodontal ligament tissues and cultured with MTA conditioned media. Cell counting kit-8 (CCK-8) assay was performed to assess the proliferation capacity of MTA-treated hPDLSCs...
July 27, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28748835/prevalence-of-lassa-virus-among-rodents-trapped-in-three-south-south-states-of-nigeria
#10
D E Agbonlahor, A Erah, I M Agba, F E Oviasogie, A F Ehiaghe, M Wankasi, O A Eremwanarue, I J Ehiaghe, E C Ogbu, R I Iyen, S Abbey, M Y Tatfeng, J Uhunmwangho
BACKGROUND & OBJECTIVES: Lassa fever has been endemic in Nigeria since 1969. The rodent Mastomys natalensis has been widely claimed to be the reservoir host of the Lassa virus. This study was designed to investigate the dis- tribution of species of rodents in three states (Edo, Delta and Bayelsa) of Nigeria and to determine the prevalence of Lassa virus amongst trapped rodents in the selected states. METHODS: Rodents were trapped during November 2015 to October 2016 from the three states in South-South re- gion of Nigeria...
April 2017: Journal of Vector Borne Diseases
https://www.readbyqxmd.com/read/28748627/genetic-ablation-of-carbonic-anhydrase-ix-disrupts-gastric-barrier-function-via-claudin-18-downregulation-and-acid-backflux
#11
Taolang Li, Xuemei Liu, Brigitte Riederer, Katerina Nikolovska, Anurag Kumar Singh, Kari A Mäkelä, Anna Seidler, Yongjian Liu, Gerolf Gros, Helmut Bartels, Karl Heinz Herzig, Ursula Seidler
AIM: The study aims to explore the molecular mechanisms for the parietal cell loss and fundic hyperplasia observed in gastric mucosa of mice lacking the carbonic anhydrase 9 (CAIX). METHODS: We assessed the ability of CAIX knockout and WT gastric surface epithelial cells to withstand luminal acid load by measuring the pHi of exteriorized gastric mucosa in vivousing two photon confocal laser scanning microscopy. Cytokines and claudin-18A2 expression was analyzed by RT-PCR...
July 27, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28748494/high-glucose-stimulates-cell-proliferation-and-collagen-iv-production-in-rat-mesangial-cells-through-inhibiting-ampk-katp-signaling
#12
Bei Zhang, Yong-Quan Shi, Jun-Jie Zou, Xiang-Fang Chen, Wei Tang, Fei Ye, Zhi-Min Liu
PURPOSE: The present study investigated the putative mechanisms underlying effects of KATP channel on high glucose (HG)-induced mesangial cell proliferation and tissue inhibitors of metalloproteinases (TIMP)-2 and Collagen IV production. METHODS: Rat mesangial cells were subjected to whole cell patch clamp to record the KATP channel currents under high glucose (HG, 30 mM) condition. Cell proliferation was measured using a CCK-8 assay. The production of TIMP-2 and Collagen IV and AMP-activated protein kinase (AMPK)-signaling pathway activity was assessed by ELISA and Western blotting, respectively...
July 26, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28748476/quantitative-rt-pcr-for-micrornas-in-biofluids
#13
Michael Thorsen, Thorarinn Blondal, Peter Mouritzen
MicroRNAs in biofluids hold great promise as minimally invasive diagnostic biomarkers for a wide range of diseases and biological processes. One of the most sensitive technologies for detection and measuring expression levels of microRNA is quantitative RT-PCR. However, quantification of microRNA in biofluid samples is challenging in many ways. Biofluids contain low levels of RNA and high levels of inhibitors of enzymatic processes like reverse transcription and PCR. Furthermore, biofluids are susceptible to many preanalytical variables...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28748470/statistical-analysis-of-quantitative-rt-pcr-results
#14
Richard Khan-Malek, Ying Wang
Quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) represents a benchmark technology in the detection and quantification of mRNA. Yet, accurate results cannot be realized without proper statistical analysis of RT-PCR data. Here we examine some of the issues concerning RT-PCR experiments that would benefit from rigorous statistical treatment including normalization, quantification, efficiency estimation, and sample size calculations. Examples are used to illustrate the methods.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28748449/first-report-of-persistent-dengue-1-associated-autoimmune-neurological-disturbance-neuromyelitis-optica-spectrum-disorder
#15
Marzia Puccioni-Sohler, Alice M M Ornelas, Andrea S de Souza, Mauro Jorge Cabral-Castro, Jessyca T M A Ramos, Carolina Rosadas, Maria Cecilia F Salgado, Alexandre A Castiglione, Fernando Ferry, Jose Mauro Peralta, Carolina Moreira Voloch, Amilcar Tanuri, Fernanda Tovar-Moll, Renato Santana Aguiar
Dengue virus (DENV) causes immune-mediated diseases. Neurological involvement represents a severe condition that is rarely observed in DENV-1 infection. Neuromyelitis optica (NMO)/NMO spectrum disorders (NMOSD) are idiopathic immune-mediated demyelinating syndromes of the central nervous system. We report a 17-year-old female with oligosymptomatic DENV-1 viremia, diagnosed as NMOSD. Magnetic resonance imaging showed spinal cord and brainstem lesions. Antibody for aquaporin 4 was negative. DENV-1 RNA infection was detected by serial RT-PCR and confirmed by phylogenetic analysis in serum...
July 26, 2017: Journal of Neurovirology
https://www.readbyqxmd.com/read/28748349/desmoplastic-small-round-cell-tumor-evaluation-of-reverse-transcription-polymerase-chain-reaction-and-fluorescence-in-situ-hybridization-as-ancillary-molecular-diagnostic-techniques
#16
Mustafa Mohamed, David Gonzalez, Karen J Fritchie, John Swansbury, Dorte Wren, Charlotte Benson, Robin L Jones, Cyril Fisher, Khin Thway
Desmoplastic small round cell tumor (DSRCT) is a rare, biologically aggressive soft tissue neoplasm of uncertain differentiation, most often arising in the abdominal and pelvic cavities of adolescents and young adults with a striking male predominance. Histologically, it is characterized by islands of uniform small round cells in prominent desmoplastic stroma, and it has a polyimmunophenotypic profile, typically expressing WT1 and cytokeratin, desmin, and neural/neuroendocrine differentiation markers to varying degrees...
July 26, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28747891/proliferative-role-of-kv11-channels-in-murine-arteries
#17
Vincenzo Barrese, Pilar Cidad, Shuk Y Yeung, Jose R López-López, Alister J McNeish, Susumu Ohya, Maria T Pérez-García, Iain A Greenwood
K(+) channels encoded by the ether-a-go-go related gene (ERG1 or KCNH2) are important determinants of the cardiac action potential. Expression of both cardiac isoforms (ERG1a and ERG1b) were identified in murine portal vein and distinctive voltage-gated K(+) currents were recorded from single myocytes. The aim of the present study was to ascertain the expression and functional impact of ERG channels in murine arteries. Methods: Quantitative RT-PCR was undertaken on RNA extracted from a number of murine arteries...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28747866/polysaccharide-from-wheat-bran-induces-cytokine-expression-via-the-toll-like-receptor-4-mediated-p38-mapk-signaling-pathway-and-prevents-cyclophosphamide-induced-immunosuppression-in-mice
#18
Ting Shen, Gongcheng Wang, Long You, Liang Zhang, Haiwei Ren, Weicheng Hu, Qian Qiang, Xinfeng Wang, Lilian Ji, Zhengzhong Gu, Xiangxiang Zhao
Wheat bran-derived polysaccharides have attracted particular attention due to their immunomodulatory effects. However, the molecular mechanisms underlying their functions are poorly understood. The current study was designed to examine the effect of wheat bran polysaccharide (WBP) on RAW 264.7 cells and the underlying signaling pathways, which have not been explored. In addition, we also investigated the immuno-enhancement effects of WBP on cyclophosphamide (CTX)-induced immunosuppression in mice. WBP significantly increased the concentrations of intracellular nitric oxide (NO) and cytokines such as prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) in RAW 264...
2017: Food & Nutrition Research
https://www.readbyqxmd.com/read/28747230/full-length-genome-sequence-analysis-of-enzootic-nasal-tumor-virus-isolated-from-goats-in-china
#19
Yapeng He, Qi Zhang, Jing Wang, Man Zhou, Mingzhe Fu, Xingang Xu
BACKGROUND: Enzootic nasal tumor virus (ENTV) is a betaretrovirus of sheep (ENTV-1) and goats (ENTV-2) associated with neoplastic transformation of epithelial cells of the ethmoid turbinate. Confirmation of the role of ENTV in the pathogenesis of enzootic nasal adenocarcinoma (ENA) has yet to be resolved due to the inability to culture the virus. Very little is known about the prevalence of this disease, particularly in China. METHODS: To evaluate the genetic diversity of ENTV-2 from Shaanxi province of China, the complete genome sequence of four isolates from Shaanxi province was determined by RT-PCR...
July 26, 2017: Virology Journal
https://www.readbyqxmd.com/read/28746985/west-nile-virus-lineage-2-in-culex-specimens-from-iran
#20
Nariman Shahhosseini, Sadegh Chinikar, Seyed Hassan Moosa-Kazemi, Mohammad Mehdi Sedaghat, Mohammad Hassan Kayedi, Renke Lühken, Jonas Schmidt-Chanasit
OBJECTIVE: Screening of mosquitoes for viruses is an important forecasting tool for emerging and reemerging arboviruses. Iran has been known to harbor medically important arboviruses such as West Nile virus (WNV) and dengue virus (DENV) based on seroepidemiological data. However, there are no data about the potential mosquito vectors for arboviruses in Iran. This study was performed to provide mosquito and arbovirus data from Iran. MATERIALS AND METHODS: 32,317 mosquitos were collected at 16 sites in 5 provinces of Iran in 2015 and 2016...
July 26, 2017: Tropical Medicine & International Health: TM & IH
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