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Thermal Imaging

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https://www.readbyqxmd.com/read/29792877/coordinating-multi-protein-mismatch-repair-by-managing-diffusion-mechanics-on-the-dna
#1
REVIEW
Daehyung Kim, Richard Fishel, Jong-Bong Lee
DNA mismatch repair (MMR) corrects DNA base pairing errors that occur during DNA replication. MMR catalyzes strand-specific DNA degradation and resynthesis by dynamic molecular coordination of sequential downstream pathways. The temporal and mechanistic order of molecular events is essential to insure interactions in MMR that occur over long distances on the DNA. Biophysical real-time studies of highly conserved components on mismatched DNA have shed light on the mechanics of MMR. Single-molecule imaging has visualized stochastically coordinated MMR interactions that are based on thermal fluctuation-driven motions...
May 21, 2018: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29791795/surface-acoustic-wave-saw-driven-device-for-dynamic-cell-cultures
#2
Gina Greco, Matteo Agostini, Ilaria Tonazzini, Damiano Sallemi, Stefano Barone, Marco Cecchini
In the last decades new types of cell cultures have been introduced to provide better cell survival and development, and micro/nano-environmental physico-chemical conditions aimed to mimic those present in vivo. However, despite the efforts made, the systems available to date are often difficult to replicate and use. Here, an easy-to-use surface acoustic wave (SAW)-based platform is presented for realizing dynamic cell cultures that is compatible with standard optical microscopes, incubators and cell culture dishes...
May 23, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29791413/measurement-of-optical-fiber-parameters-and-thermal-core-diffusion-characteristics-by-digital-image-processing
#3
F Chavez-Gutierrez, A Martinez-Rios, D Toral-Acosta, D Torres-Armenta, J A Guerrero-Viramontes
The simultaneous measurement of the numerical aperture (NA) and mode field diameter (MFD) of single-mode fibers was performed on several commercial fibers by means of digital image processing of the output light projected into a screen. In addition, the changes induced by core diffusion in an ultra-high (UH) NA fiber at several heat depositions from a CO2 laser source were measured. The measurement method was demonstrated to be useful to monitor the adiabaticity of this process, by taking the product of the NA and MFD to verify if it remains constant...
May 20, 2018: Applied Optics
https://www.readbyqxmd.com/read/29786615/morphological-control-of-gold-nanorods-via-thermally-driven-bi-surfactant-growth-and-application-for-detection-of-heavy-metal-ions
#4
Hao Huang, Huiyi Li, Huaiyu Wang, Jia Li, Penghui Li, Qidan Chen, Yue Chen, Paul K Chu, Bo Li, Xuefeng Yu
We report a modified synthesis route of colloidal gold nanorods (AuNRs) by combining the thermal re-shaping treatment and bi-surfactant modification using hexadecyltrimethylammonium bromide (CTAB) and sodium oleate (NaOL). Aspect ratios down to 1.3 ± 0.1 can be achieved in addition to good monodispersity, uniformity, and chemical stability of the materials. Furthermore, without needing post-treatment, metal ions directly interact with the AuNRs efficiently, allowing rapid and sensitive colorimetric detection of heavy metal ions such as Pb<sup>2+</sup> and Cu<sup>2+</sup> with a low concentration down to 2...
May 22, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29782671/enhanced-performance-of-a-molecular-photoacoustic-imaging-agent-by-encapsulation-in-mesoporous-silicon-nanoparticles
#5
Jinyoung Kang, Dokyoung Kim, Junxin Wang, Yunho Han, Jonathan M Zuidema, Ali Hariri, Ji-Ho Park, Jesse V Jokerst, Michael J Sailor
Photoacoustic (PA) imaging allows visualization of the physiology and pathology of tissues with good spatial resolution and relatively deep tissue penetration. The method converts near-infrared (NIR) laser excitation into thermal expansion, generating pressure transients that are detected with an acoustic transducer. Here, we find that the response of the PA contrast agent indocyanine green (ICG) can be enhanced 17-fold when it is sealed within a rigid nanoparticle. ICG encapsulated in particles composed of porous silicon (pSiNP), porous silica, or calcium silicate all show greater PA contrast relative to equivalent quantities of free ICG, with the pSiNPs showing the strongest enhancement...
May 21, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29782243/thermal-hazard-for-mr-imaging-systems-includes-all-textiles-with-metal
#6
Daniel M Krainak, Jana G Delfino, Terry O Woods
No abstract text is available yet for this article.
June 2018: Radiology
https://www.readbyqxmd.com/read/29781118/ballistic-tongue-projection-in-a-miniaturized-salamander
#7
Stephen M Deban, Segall V Bloom
Miniaturization of body size is often accompanied by peculiarities in morphology that can have functional consequences. We examined the feeding behavior and morphology of the miniaturized plethodontid salamander Thorius, one of the smallest vertebrates, to determine if its performance and biomechanics differ from those of its larger relatives. High-speed imaging and dynamics analysis of feeding at a range of temperatures show that tongue projection in Thorius macdougalli is ballistic and achieves accelerations of up to 600 G with low thermal sensitivity, indicating that tongue projection is powered by an elastic-recoil mechanism...
May 20, 2018: Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology
https://www.readbyqxmd.com/read/29780915/solitary-retinal-hemangioblastoma-findings-in-octa-pre-and-post-laser-therapy
#8
Brian W Chou, Peter L Nesper, Lee M Jampol, Rukhsana G Mirza
Purpose: To report optical coherence tomography angiography (OCTA) findings pre- and post-thermal laser therapy of a rare, asymptomatic, unilateral, isolated retinal hemangioblastoma. Observations: A 40-year-old asymptomatic Caucasian man was found to have an isolated, unilateral retinal hemangioblastoma after referral for possible retinal hole. Comparison of OCTAs pre- and post-thermal laser therapy demonstrated a significant reduction of blood flow toward and within the lesion, in addition to contraction of the lesion and constriction of the accompanying feeder vessels...
June 2018: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/29778037/efficient-electrochemical-degradation-of-multiwall-carbon-nanotubes
#9
Vytas Reipa, Shannon K Hanna, Aaron Urbas, Lane Sander, John Elliott, Joseph Conny, Elijah J Petersen
As the production mass of multiwall carbon nanotubes (MWCNT) increases, the potential for human and environmental exposure to MWCNTs may also increase. We have shown that exposing an aqueous suspension of pristine MWCNTs to an intense oxidative treatment in an electrochemical reactor, equipped with an efficient hydroxyl radical generating Boron Doped Diamond (BDD) anode, leads to their almost complete mineralization. Thermal optical transmittance analysis showed a total carbon mass loss of over two orders of magnitude due to the electrochemical treatment, a result consistent with measurements of the degraded MWCNT suspensions using UV-vis absorbance...
April 26, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29776832/an-sar-study-of-hydroxy-trifluoromethylpyrazolines-as-inhibitors-of-orai1-mediated-store-operated-ca-2-entry-in-mda-mb-231-breast-cancer-cells-using-a-convenient-fluorescence-imaging-plate-reader-assay
#10
Ralph J Stevenson, Iman Azimi, Jack U Flanagan, Marco Inserra, Irina Vetter, Gregory R Monteith, William A Denny
The proteins Orai1 and STIM1 control store-operated Ca2+ entry (SOCE) into cells. SOCE is important for migration, invasion and metastasis of MDA-MB-231 human triple negative breast cancer (TNBC) cells and has been proposed as a target for cancer drug discovery. Two hit compounds from a medium throughput screen, displayed encouraging inhibition of SOCE in MDA-MB-231 cells, as measured by a Fluorescence Imaging Plate Reader (FLIPR) Ca2+ assay. Following NMR spectroscopic analysis of these hits and reassignment of their structures as 5-hydroxy-5-trifluoromethylpyrazolines, a series of analogues was prepared via thermal condensation reactions between substituted acylhydrazones and trifluoromethyl 1,3-dicarbonyl arenes...
May 9, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29776438/uninterrupted-monitoring-of-drug-effects-in-human-induced-pluripotent-stem-cell-derived-cardiomyocytes-with-bioluminescence-ca-2-microscopy
#11
Kazushi Suzuki, Takahito Onishi, Chieko Nakada, Shunsuke Takei, Matthew J Daniels, Masahiro Nakano, Tomoki Matsuda, Takeharu Nagai
OBJECTIVE: Cardiomyocytes derived from human-induced pluripotent stem cells are a powerful platform for high-throughput drug screening in vitro. However, current modalities for drug testing, such as electrophysiology and fluorescence imaging have inherent drawbacks. To circumvent these problems, we report the development of a bioluminescent Ca2+ indicator GmNL(Ca2+ ), and its application in a customized microscope for high-throughput drug screening. RESULTS: GmNL(Ca2+ ) gives a 140% signal change with Ca2+ , and can image drug-induced changes of Ca2+ dynamics in cultured cells...
May 18, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29775911/estimation-of-collagen-presence-in-dry-bone-using-combined-x-ray-and-neutron-radiography
#12
Arkadiusz Sołtysiak, Ewelina A Miśta-Jakubowska, Michał Dorosz, Tymoteusz Kosiński, Izabela Fijał-Kirejczyk
Thermal neutron radiography and X-ray radiography are characterised by different penetration depths in various materials, for example in collagen and hydroxyapatite, two major components of bone. Neutron radiography penetrates hydroxyapatite easier than collagen and, conversely, in X-ray radiography attenuation is higher in hydroxyapatite than in collagen. This effect allows estimation of collagen presence in dry bone. In our study we show that differences between the two imaging methods are sufficient to produce significant results when bone areas with higher and lower content of collagen are being compared...
March 30, 2018: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/29775440/crystalline-multiwall-carbon-nanotube-and-its-application-as-field-emission-electron-source
#13
Peng Liu, Duanliang Zhou, Chunhai Zhang, Haoming Wei, Xinhe Yang, Yang Wu, Qingwei Li, Changhong Liu, Bingchu Du, Liang Liu, Kaili Jiang, Shoushan Fan
With the super aligned carbon nanotube (SACNT) film, we have fabricated crystalline multiwall CNT (MWCNT) by adopting a high pressure and high temperature processing. The CNTs keep parallel to each other and distributed uniformly. XRD characterization shows peaks at small angle range, which can be assigned to the spacing of MWCNT crystal. The mechanical, electrical and thermal properties are all greatly improved compared with the original CNT film. The field emission properties of crystalline MWCNT are tested and it shows a better surface morphology stability at large emission current...
May 18, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29774894/value-of-diffusion-weighted-imaging-for-monitoring-tissue-change-during-magnetic-resonance-guided-high-intensity-focused-ultrasound-therapy-in-bone-applications-an-ex-vivo-study
#14
Sharon L Giles, Jessica M Winfield, David J Collins, Ian Rivens, John Civale, Gail R Ter Haar, Nandita M deSouza
Background: Magnetic resonance (MR)-guided high-intensity focused ultrasound (HIFU) can palliate metastatic bone pain by periosteal neurolysis. We investigated the value of diffusion-weighted imaging (DWI) for monitoring soft tissue changes adjacent to bone during MR-guided HIFU. We evaluated the repeatability of the apparent diffusion coefficient (ADC) measurement, the temporal evolution of ADC change after sonication, and its relationship with thermal parameters. Methods: Ex-vivo experiments in lamb legs ( n = 8) were performed on a Sonalleve MR-guided HIFU system...
2018: European radiology experimental
https://www.readbyqxmd.com/read/29773363/characterization-of-a-fluorescent-hydrogel-synthesized-using-chitosan-polyvinyl-alcohol-and-9-anthraldehyde-for-the-selective-detection-and-discrimination-of-trace-fe-3-and-fe-2-in-water-for-live-cell-imaging
#15
Santu Maity, Nira Parshi, Chandraday Prodhan, Keya Chaudhuri, Jhuma Ganguly
A three-dimensional fluorescent hydrogel based on chitosan, polyvinyl alcohol and 9-anthraldehyde (ChPA) has been successfully designed and synthesized for the selective detection and discrimination of Fe3+ and Fe2+ in aqueous environment. The unique characteristics of ChPA has been confirmed by the Fourier-transform infrared spectroscopy (FTIR), rheological measurement, scanning electron microscopy (SEM), thermogravimetry and differential thermogravimetry (TG-DTG), ultraviolet-visible spectroscopy (UV-vis), fluorescence studies, transmission electron microscopy (TEM), energy dispersive x-ray spectroscopy (EDX), x-ray diffraction (XRD) and dynamic light scattering (DLS)...
August 1, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29772689/fabrication-and-characterization-of-nanoenergetic-hollow-spherical-hexanitrostibene-hns-derivatives
#16
Xiong Cao, Peng Deng, Shuangqi Hu, Lijun Ren, Xiaoxia Li, Peng Xiao, Yu Liu
The spherization of nanoenergetic materials is the best way to improve the sensitivity and increase loading densities and detonation properties for weapons and ammunition, but the preparation of spherical nanoenergetic materials with high regularization, uniform size and monodispersity is still a challenge. In this paper, nanoenergetic hollow spherical hexanitrostibene (HNS) derivatives were fabricated via a one-pot copolymerization strategy, which is based on the reaction of HNS and piperazine in acetonitrile solution...
May 16, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29772066/3d-specific-absorption-rate-estimation-from-high-intensity-focused-ultrasound-sonications-using-the-green-s-function-heat-kernel
#17
Nicholas J Freeman, Henrik Odéen, Dennis L Parker
PURPOSE: Evaluate a numerical inverse Green's function method of deriving specific absorption rates (SARs) from high intensity focused ultrasound (HIFU) sonications given tissue parameters (thermal conductivity, specific heat capacity, and mass density) and 3-D magnetic resonance imaging (MRI) temperature measurements. METHODS: SAR estimates were evaluated using simulations and MR temperature measurements from HIFU sonications. For simulations, a "true" SAR was calculated using the hybrid angular spectrum method for ultrasound simulations...
May 17, 2018: Medical Physics
https://www.readbyqxmd.com/read/29770773/highly-ordered-molecular-rotor-matrix-on-a-nanopatterned-template-titanyl-phthalocyanine-molecules-on-feo-pt-111
#18
Shuangzan Lu, Min Huang, Zhihui Qin, Yinghui Yu, Qinmin Guo, Gengyu Cao
Molecular rotors, motors and gears plays important roles in artificial molecular machines, in which rotor and motor matrices are much desirable for large scale bottom-up fabrication of molecular machines. Here we demonstrate the fabrication of a highly ordered molecular rotor matrix by depositing nonplanar dipolar titanyl phthalocyanine (TiOPc, C<sub>32</sub>H<sub>16</sub>N<sub>8</sub>OTi) molecules on Moiré patterned dipolar FeO/Pt(111) substrate...
May 17, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29770422/biodegradable-toughened-nanohybrid-shape-memory-polymer-for-smart-biomedical-applications
#19
Arpan Biswas, Akhand Pratap Singh, Dipak Rana, Vinod K Aswal, Pralay Maiti
A polyurethane nanohybrid has been prepared through the in situ polymerization of an aliphatic diisocyanate, ester polyol and a chain extender in the presence of two-dimensional platelets. Polymerization within the platelet galleries helps to intercalate, generate diverse nanostructure and improve the nano to macro scale self-assembly, which leads to a significant enhancement in the toughness and thermal stability of the nanohybrid in comparison to pure polyurethane. The extensive interactions, the reason for property enhancement, between nanoplatelets and polymer chains are revealed through spectroscopic measurements and thermal studies...
May 17, 2018: Nanoscale
https://www.readbyqxmd.com/read/29768878/nano-nets-covered-composite-nanofibers-with-enhanced-biocompatibility-and-mechanical-properties-for-bone-tissue-engineering
#20
Arjun Prasad Tiwari, Mahesh Kumar Joshi, Chan Hee Park, Cheol Sang Kim
Enhancing the biocompatibility profiles including cell attachment, growth, and viability and mechanical properties of designed synthetic scaffolds have an essential role in tissue engineering applications. Polymer blending is one of the most effective methods for providing new anticipated biomaterials for tissue scaffolds. Here, the blend solution of the different mass weight ratio of polycaprolactone (PCL) to human serum albumin (HSA) was subjected to fabricate nanocomposite spider-web-like membranes using electrospinning process...
January 1, 2018: Journal of Nanoscience and Nanotechnology
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