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https://www.readbyqxmd.com/read/27936518/computational-investigation-of-proton-transfer-pka-shifts-and-ph-optimum-of-protein-dna-and-protein-rna-complexes
#1
Yunhui Peng, Emil Alexov
Protein-nucleic acid interactions play a crucial role in many biological processes. This work investigates the changes of pKa values and protonation states of ionizable groups (including nucleic acid bases) that may occur at protein-nucleic acid binding. Taking advantage of the recently developed pKa calculation tool DelphiPka, we utilize the large protein-nucleic acid interaction database (NPIDB database) to model pKa shifts caused by binding. It has been found that the protein's interfacial basic residues experience favorable electrostatic interactions while the protein acidic residues undergo proton uptake to reduce the energy cost upon the binding...
December 9, 2016: Proteins
https://www.readbyqxmd.com/read/27935111/degradation-of-elastic-fiber-and-elavated-elastase-expression-in-long-head-of-biceps-tendinopathy
#2
Yen-Ting Wu, Wei-Ren Su, Po-Ting Wu, Po-Chuan Shen, I-Ming Jou
Tendinopathy of the long head of the biceps (TLHB) involves various types of extracellular matrix degeneration, but previous studies have not evaluated elastic fibers. The purpose of this study was to investigate elastic fiber distribution in long head of the biceps (LHB). The TLHB tendons of 16 consecutive patients (8 men and 8 women; average age of 55.75 years; age range of 40-71 years) were transected and harvested. Three cadaveric LHB tendons were used as the control group. The expression of collagen type I was decreased, but type III was increased in TLHB...
December 9, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27932432/fungal-mobile-mass-on-echocardiogram-native-mitral-valve-aspergillus-fumigatus-endocarditis
#3
Rymon Rofaiel, Yosra Turkistani, David McCarty, Seyed M Hosseini-Moghaddam
The most common type of infective endocarditis is bacterial endocarditis. However, fungal infections have been seen more frequently, mostly in the immunocompromised population. We report a case of invasive Aspergillus fumigatus native mitral valve endocarditis. The patient received appropriate empiric antifungal treatment with a combination of liposomal amphotericin B and flucytosine, associated with surgical debridement, valve replacement and chordae tendineae repair. Despite receiving the standard treatment of Aspergillus endocarditis, and susceptibility of the microorganism to the antifungal regimen, the patient, unexpectedly, developed early-onset septic emboli...
December 8, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27930691/vascular-endothelial-growth-factor-receptor-2-polymorphisms-have-protective-effect-against-the-development-of-tendinopathy-in-volleyball-athletes
#4
José Inácio Salles, Maria Eugenia Leite Duarte, João Matheus Guimarães, Lucas Rafael Lopes, Jessica Vilarinho Cardoso, Diego Pinheiro Aguiar, João Olyntho Machado Neto, Daniel Escorsim Machado, Jamila Alessandra Perini
The aim of the study was to investigate whether genetic variants in VEGF and KDR genes can be correlated with susceptibility of tendinopathy in volleyball athletes. This study was conducted at the Brazilian Volleyball Federation, and comprised 179 volleyball athletes: 88 had a confirmed diagnosis of tendinopathy (cases), whereas 91 had no evidence of the disease (controls). The VEGF (-2578C>A, -460T>C and +936C>T) and KDR (-604C>T, 1192G>A and 1719T>A) polymorphisms were determined by TaqMan real-time polymerase chain reaction...
2016: PloS One
https://www.readbyqxmd.com/read/27930090/sonoelastography-of-the-common-flexor-tendon-of-the-elbow-with-histologic-agreement-a-cadaveric-study
#5
Andrea S Klauser, Mathias J Pamminger, Ethan J Halpern, Mohamed M H Abd Ellah, Bernhard Moriggl, Mihra S Taljanovic, Christian Deml, Judith Sztankay, Guenter Klima, Leonhard Gruber, Werner R Jaschke
Purpose To determine the correlation of the results of conventional B-mode ultrasonography (US) and compression sonoelastography with histologic results in common flexor tendons of the elbow in human cadavers. Materials and Methods Twenty-five common flexor tendons were evaluated in 16 fresh, unembalmed cadavers of 11 women with a median age of 85 years (range, 71-101 years) and five men with a median age of 78 years (range, 70-88 years). Informed consent was provided according to the last will of the donors...
December 7, 2016: Radiology
https://www.readbyqxmd.com/read/27928439/coping-behavior-and-risk-and-resilience-stress-factors-in-french-regional-emergency-medicine-unit-workers-a-cross-sectional-survey
#6
Lala Ai, Sturzu Lm, Picard Jp, Druot F, Grama F, Bobirnac G
The Emergency Department (ED) has the highest workload in a hospital, offering care to patients in their most acute state of illness, as well as comforting their families and tending to stressful situations of the physical and psychological areal. Method. A cross-sectional survey of 366 Emergency Unit staff members including medical doctors, medical residents, medical nurses and ward aids, was undergone. Study participants came from four periphery hospitals in the Moselle Department of Eastern France with similar workforce and daily patient loads statistics...
October 2016: Journal of Medicine and Life
https://www.readbyqxmd.com/read/27920330/treatment-of-insertional-achilles-pathology-with-dorsal-wedge-calcaneal-osteotomy-in-athletes
#7
Dimitrios Georgiannos, Vasilis Lampridis, Angelos Vasiliadis, Ilias Bisbinas
BACKGROUND: Insertional Achilles tendinopathy and retrocalcaneal bursitis is difficult to treat, and several operative techniques have been used after failure of conservative management. Dorsal wedge calcaneal osteotomy has been described for the treatment of insertional Achilles pathology. It was hypothesized that dorsal wedge calcaneal osteotomy would be an effective and safe method for the treatment of athletes with insertional Achilles pathology unrelieved by nonoperative measures...
December 5, 2016: Foot & Ankle International
https://www.readbyqxmd.com/read/27918676/effects-of-training-load-and-leg-dominance-on-achilles-and-patellar-tendon-structure
#8
Alireza Esmaeili, Andrew M Stewart, William G Hopkins, George P Elias, Robert J Aughey
PURPOSE: Detrimental changes in tendon structure increase the risk of tendinopathies. The aim of this study was to investigate the influence of individual internal and external training loads and leg dominance on changes in the Achilles and patellar tendon structure. METHODS: The internal structure of the Achilles and patellar tendons of both limbs of 26 elite Australian footballers was assessed using ultrasound tissue characterization at the beginning and the end of an 18 week pre-season...
December 5, 2016: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/27914921/polydeoxyribonucleotide-injection-in-the-treatment-of-chronic-supraspinatus-tendinopathy-a-case-controlled-retrospective-comparative-study-of-6-month-follow-up
#9
Young Chun Yoon, Doo-Hyung Lee, Michael Young Lee, Seung-Hyun Yoon
OBJECTIVE: To determine the efficacy of polydeoxyribonucleotide (PDRN) injection for rotator cuff disease (RCD). DESIGN: Case-controlled, retrospective, comparative study. SETTING: Outpatient clinic at university-affiliated tertiary care hospital. PARTICIPANTS: 106 patients with chronic non-traumatic refractory RCD who were unresponsive to at least 1 month of conservative treatment; 55 patients received PDRN injection (PDRN group) and 51 continued conservative treatment (control group)...
November 30, 2016: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/27909786/calcific-tendinopathy-of-the-shoulder-with-intraosseous-extension-outcomes-of-ultrasound-guided-percutaneous-irrigation
#10
Michail E Klontzas, Evangelia E Vassalou, Apostolos H Karantanas
OBJECTIVES: Rotator cuff calcific tendinopathy (RCCT) with intraosseous extension is a rare complication of tendinous and peritendinous involvement. The purpose of our study is to evaluate the outcome of ultrasound-guided percutaneous irrigation of calcific tendinopathy (US-PICT) in patients with intraosseous involvement. MATERIALS AND METHODS: From January 2011 to June 2014, patients with a clinical and imaging diagnosis of RCCT were prospectively categorised in two groups based on imaging findings: group A (10 patients) with intraosseous RCCT and group B (control group 35 patients) without osseous involvement...
December 1, 2016: Skeletal Radiology
https://www.readbyqxmd.com/read/27909679/arthroscopic-treatment-of-patellar-tendinopathy-use-of-70%C3%A2-arthroscope-and-superolateral-portal
#11
Orestis Karargyris, Vipul Mandalia
Surgical treatment of recalcitrant patellar tendinopathy includes both the open surgical approach and minimally invasive arthroscopic debridement. A variety of arthroscopic techniques have been described that involve the use of a standard 30° arthroscope and standard anterolateral and anteromedial portals. As a result, visualization of the infrapatellar region can be variable, and it may be necessary to create additional portals. A 70° arthroscope provides the advantage of a wider field of view to the surgeon...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27909678/bridge-tenodesis-a-secure-fixation-technique-for-biceps-long-head-tendinopathy-during-arthroscopic-rotator-cuff-repair-using-a-suture-bridge-technique
#12
Jin-Young Park, Jae-Hyung Lee, Kyung-Soo Oh, Seok-Won Chung, Jin-Young Bang, Young-Min Noh
Tendinopathy of the long head of the biceps is often found as an intra-articular pathology in the glenohumeral joint. Because long head of the biceps lesions are common, surgical intervention to properly manage the long head of the biceps has become an important issue. Both tenodesis and tenotomy have been shown to provide benefits in biceps long head tendinopathy. But because of concerns about muscle power reduction, cramping, and "Popeye's deformity," which may result from biceps tenotomy, biceps tenodesis is a good option for treating biceps lesions...
October 2016: Arthroscopy Techniques
https://www.readbyqxmd.com/read/27904957/neck-pain-and-acute-dysphagia
#13
João Simões, José Romão, Anita Cunha, Sofia Paiva, António Miguéis
The acute tendinitis of the longus colli muscle is an unusual diagnosis in the cases of acute dysphagia with cervical pain. Is a self-limiting condition caused by abnormal calcium hydroxyapatite deposition in the prevertebral space and can cause pharyngeal swelling with impaired swallow. It is absolutely critical to make the differential diagnosis with deep cervical infections in order to avoid invasive treatments.
November 30, 2016: Dysphagia
https://www.readbyqxmd.com/read/27904789/current-concepts-in-the-treatment-of-patellar-tendinopathy
#14
Mark F Reinking
: Patellar tendon pain is a significant problem in athletes who participate in jumping and running sports and can interfere with athletic participation. This clinical commentary reviews patellar tendon anatomy and histopathology, the language used to describe patellar tendon pathology, risk factors for patellar tendinopathy and common interventions used to address patellar tendon pain. Evidence is presented to guide clinicians in their decision-making regarding the treatment of athletes with patellar tendon pain...
December 2016: International Journal of Sports Physical Therapy
https://www.readbyqxmd.com/read/27904212/functional-outcome-after-primary-hemiarthroplasty-in-three-or-four-part-proximal-humerus-fracture-a-short-term-followup
#15
Saurabh Agarwal, Ashish Rana, Rajeev K Sharma
BACKGROUND: Several modalities of treatment are being used for the management of proximal humerus fractures. Primary hemiarthroplasty in proximal humerus fracture is indicated in three or four part fracture or fracture dislocations. It is also indicated if fracture involves a large area of articular cartilage loss and viability of head is doubtful. We studied the functional outcome of hemiarthroplasty in comminuted proximal humerus fracture. MATERIALS AND METHODS: 29 patients of three or four part proximal humerus fractures, (according to Neer's classification) who underwent primary shoulder hemiarthroplasty were included in this retrospective study...
November 2016: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/27904023/early-embryonic-development-of-long-tendons-in-the-human-foot
#16
Masahito Yamamoto, Yasuhiro Shiraishi, Kei Kitamura, Zhe Wu Jin, Gen Murakami, Jose Francisco Rodríguez-Vázquez, Shin-Ichi Abe
To examine a common plantar tendinous plate for long flexors of the toe and fingers in human embryos, we observed sections of 10 embryos at 5-6 weeks (crown-rump length or CRL 15-21 mm). The heel or tuber of the calcaneus was underdeveloped in 3 embryos with CRL 15 mm and the talus appeared not to be piled up on the calcaneus but these two bones were arranged along the lateromedial axis. As reported in the hand, we demonstrated, in the deep side of tarsal bones, a common tendinous plate formed by a joining of the flexor halluces longus and flexor digitorum longus tendons...
2016: Okajimas Folia Anatomica Japonica
https://www.readbyqxmd.com/read/27900924/-persistent-shoulder-symptoms-in-calcific-tendinitis-clinical-and-radiological-predictors
#17
P B de Witte, R A van Adrichem, J W Selten, J Nagels, M Reijnierse, R G H H Nelissen
OBJECTIVE: We assessed the most important demographics and radiological characteristics at the time of diagnosis of rotator cuff calcific tendinitis (RCCT), and their associations with long-term clinical outcome. DESIGN: Observational study. METHOD: Baseline characteristics and treatment were evaluated in 342 patients in whom RCCT had been diagnosed. Interobserver agreement of the radiological investigations was analysed. Patients were sent a general questionnaire and 2 shoulder questionnaires, the "Western Ontario rotator cuff" (WORC) and the "Disabilities of the arm, shoulder and hand" (DASH) for evaluation of long-term clinical outcome...
2016: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/27900300/treatment-of-proximal-hamstring-tendinopathy-related-sciatic-nerve-entrapment-presentation-of-an-ultrasound-guided-intratissue-percutaneous-electrolysis-application
#18
Gabriele Mattiussi, Carlos Moreno
BACKGROUND: Proximal Hamstring Tendinopathy-related Sciatic Nerve Entrapment (PHTrSNE) is a neuropathy caused by fibrosis interposed between the semimembranosus tendon and the sciatic nerve, at the level of the ischial tuberosity. METHODS: Ultrasound-guided Intratissue Percutaneous Electrolysis (US-guided EPI) involves galvanic current transfer within the treatment target tissue (fibrosis) via a needle 0.30 to 0.33 mm in diameter. The galvanic current in a saline solution instantly develops the chemical process of electrolysis, which in turn induces electrochemical ablation of fibrosis...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27900294/efficacy-of-autologous-leukocyte-reduced-platelet-rich-plasma-therapy-for-patellar-tendinopathy-in-a-rat-treadmill-model
#19
Mamoru Yoshida, Hiroki Funasaki, Keishi Marumo
BACKGROUND: An autologous platelet-rich plasma (PRP) therapy has currently been applied for the tendinopathy; however, its efficacy and an optimal platelets concentration in PRP were uncertain. We analyzed them in an animal model prepared using a repetitive running exercise. METHODS: We made the tendinopathy rat model of patellar tendon using a rodent treadmill machine. Rats with tendinopathy were injected with leukocyte-reduced PRP at the platelets concentration of 1...
April 2016: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/27900292/hypoxia-inhibits-primary-cilia-formation-and-reduces-cell-mediated-contraction-in-stress-deprived-rat-tail-tendon-fascicles
#20
Michael Lavagnino, Anna N Oslapas, Keri L Gardner, Steven P Arnoczky
BACKGROUND: Hypoxia, which is associated with chronic tendinopathy, has recently been shown to decrease the mechanosensitivity of some cells. Therefore, the purpose of this study was to determine the effect of hypoxia on the formation of elongated primary cilia (a mechanosensing organelle of tendon cells) in vitro and to determine the effect of hypoxia on cell-mediated contraction of stress-deprived rat tail tendon fascicles (RTTfs). METHODS: Tendon cells isolated from RTTfs were cultured under normoxic (21% O2) or hypoxic (1% O2) conditions for 24 hours...
April 2016: Muscles, Ligaments and Tendons Journal
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