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beauveria bassiana gene

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https://www.readbyqxmd.com/read/28916147/microbial-associates-of-the-southern-mole-cricket-scapteriscus-borellii-are-highly-pathogenic
#1
Sudarshan K Aryal, Derreck Carter-House, Jason E Stajich, Adler R Dillman
We report the isolation and identification of seven bacterial strains and one fungal strain from dead and diseased Scapteriscus borellii mole crickets collected from a golf course in southern California. Using 16S and 18S rRNA gene sequence analysis we identified the microbes as Serratia marcescens (red), S. marcescens (white), S. marcescens (purple), Achromobacter xylosoxidans, Chryseobacterium sp., Ochrobactrum anthropi, Tsukamurella tryosinosolvens, and Beauveria bassiana. We performed a dose response curve for each of these cricket-associated microbial strains (except T...
September 12, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28873459/divergent-lysm-effectors-contribute-to-the-virulence-of-beauveria-bassiana-by-evasion-of-insect-immune-defenses
#2
Kai Cen, Bing Li, Yuzhen Lu, Siwei Zhang, Chengshu Wang
The lysin motif (LysM) containing proteins can bind chitin and are ubiquitous in various organisms including fungi. In plant pathogenic fungi, a few LysM proteins have been characterized as effectors to suppress chitin-induced immunity in plant hosts and therefore contribute to fungal virulence. The effector mechanism is still questioned in fungus-animal interactions. In this study, we found that LysM proteins are also present in animal pathogenic fungi and have evolved divergently. The genome of the insect pathogen Beauveria bassiana encodes 12 LysM proteins, and the genes were differentially transcribed by the fungus when grown in different conditions...
September 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28871395/interactome-analysis-of-transcriptional-coactivator-multiprotein-bridging-factor-1-unveils-a-yeast-ap-1-like-transcription-factor-involved-in-oxidation-tolerance-of-mycopathogen-beauveria-bassiana
#3
Xin-Ling Chu, Wei-Xia Dong, Jin-Li Ding, Ming-Guang Feng, Sheng-Hua Ying
Oxidation tolerance is an important determinant to predict the virulence and biocontrol potential of Beauveria bassiana, a well-known entomopathogenic fungus. As a transcriptional coactivator, multiprotein bridging factor 1 mediates the activity of transcription factor in diverse physiological processes, and its homolog in B. bassiana (BbMBF1) contributes to fungal oxidation tolerance. In this study, the BbMBF1-interactomes under oxidative stress and normal growth condition were deciphered by mass spectrometry integrated with the immunoprecipitation...
September 4, 2017: Current Genetics
https://www.readbyqxmd.com/read/28870289/ethanol-production-from-chitosan-by-the-nematophagous-fungus-pochonia-chlamydosporia-and-the-entomopathogenic-fungi-metarhizium-anisopliae-and-beauveria-bassiana
#4
Almudena Aranda-Martinez, Miguel Ángel Naranjo Ortiz, Isabel Sofía Abihssira García, Ernesto A Zavala-Gonzalez, Luis Vicente Lopez-Llorca
Chitin is the second most abundant biopolymer after cellulose and virtually unexplored as raw material for bioethanol production. In this paper, we investigate chitosan, the deacetylated form of chitin which is the main component of shellfish waste, as substrate for bioethanol production by fungi. Fungal parasites of invertebrates such as the nematophagous Pochonia chlamydosporia (Pc) or the entomopathogens Beauveria bassiana (Bb) and Metarhizium anisopliae (Ma) are biocontrol agents of plant parasitic nematodes (eg...
November 2017: Microbiological Research
https://www.readbyqxmd.com/read/28762123/differential-immune-responses-of-monochamus-alternatus-against-symbiotic-and-entomopathogenic-fungi
#5
Wei Zhang, Jie Meng, Jing Ning, Peijun Qin, Jiao Zhou, Zhen Zou, Yanhong Wang, Hong Jiang, Faheem Ahmad, Lilin Zhao, Jianghua Sun
Monochamus alternatus, the main vector beetles of invasive pinewood nematode, has established a symbiotic relationship with a native ectotrophic fungal symbiont, Sporothrix sp. 1, in China. The immune response of M. alternatus to S. sp. 1 in the coexistence of beetles and fungi is, however, unknown. Here, we report that immune responses of M. alternatus pupae to infection caused by ectotrophic symbiotic fungus S. sp. 1 and entomopathogenic fungus Beauveria bassiana differ significantly. The S. sp. 1 did not kill the beetles while B...
July 28, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28755300/in-vivo-gene-expression-profiling-of-the-entomopathogenic-fungus-beauveria-bassiana-elucidates-its-infection-stratagems-in-anopheles-mosquito
#6
Yiling Lai, Huan Chen, Ge Wei, Guandong Wang, Fang Li, Sibao Wang
The use of entomopathogenic fungi to control mosquitoes is a promising tool for reducing vector-borne disease transmission. To better understand infection stratagems of insect pathogenic fungi, we analyzed the global gene expression profiling of Beauveria bassiana at 36, 60, 84 and 108 h after topical infection of Anopheles stephensi adult mosquitoes using RNA sequencing (RNA-Seq). A total of 5,354 differentially expressed genes (DEGs) are identified over the course of fungal infection. When the fungus grows on the mosquito cuticle, up-regulated DEGs include adhesion-related genes involved in cuticle attachment, Pth11-like GPCRs hypothesized to be involved in host recognition, and extracellular enzymes involved in the degradation and penetration of the mosquito cuticle...
July 27, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/28730600/transcriptomic-insights-into-the-alternative-splicing-mediated-adaptation-of-the-entomopathogenic-fungus-beauveria-bassiana-to-host-niches-autophagy-related-gene-8-as-an-example
#7
Wei-Xia Dong, Jin-Li Ding, Yang Gao, Yue-Jin Peng, Ming-Guang Feng, Sheng-Hua Ying
Alternative splicing (AS) regulates various biological processes in fungi by extending the cellular proteome. However, comprehensive studies investigating AS in entomopathogenic fungi are lacking. Based on transcriptome data obtained via dual RNA-seq, the first overview of AS events was developed for Beauveria bassiana growing in an insect hemocoel. The AS was demonstrated for 556 of 8,840 expressed genes, accounting for 5.4% of the total genes in B. bassiana. Intron retention was the most abundant type of AS, accounting for 87...
July 21, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28713011/the-genomic-and-transcriptomic-analyses-of-serine-proteases-and-their-homologs-in-an-endoparasitoid-pteromalus-puparum
#8
Lei Yang, Zhe Lin, Qi Fang, Jiale Wang, Zhichao Yan, Zhen Zou, Qisheng Song, Gongyin Ye
In insects, serine proteases (SPs) and serine protease homologs (SPHs) constitute a large family of proteins involved in multiple physiological processes such as digestion, development, and immunity. Here we identified 145 SPs and 38 SPHs in the genome of an endoparasitoid, Pteromalus puparum. Gene duplication and tandem repeats were observed in this large SPs/SPHs family. We then analyzed the expression profiles of SP/SPH genes in response to different microbial infections (Gram-positive bacterium Micrococcus luteus, Gram-negative bacterium Escherichia coli, and entomopathogenic fungus Beauveria bassiana), as well as in different developmental stages and tissues...
July 13, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28708056/metarhizium-robertsii-produces-indole-3-acetic-acid-which-promotes-root-growth-in-arabidopsis-and-enhances-virulence-to-insects
#9
Xinggang Liao, Brian Lovett, Weiguo Fang, Raymond J St Leger
The plant root colonizing insect-pathogenic fungus Metarhizium robertsii has been shown to boost plant growth, but little is known about the responsible mechanisms. Here we show that M. robertsii promotes lateral root growth and root hair development of Arabidopsis seedlings in part through an auxin [indole-3-acetic acid (IAA)]-dependent mechanism. M. robertsii, or its auxin-containing culture filtrate promoted root proliferation, activated IAA-regulated gene expression and rescued the root hair defect of the IAA-deficient rhd6 Arabidopsis mutant...
July 15, 2017: Microbiology
https://www.readbyqxmd.com/read/28706459/beauvericin-a-cyclic-peptide-inhibits-inflammatory-responses-in-macrophages-by-inhibiting-the-nf-%C3%AE%C2%BAb-pathway
#10
Sulgi Yoo, Mi-Yeon Kim, Jae Youl Cho
Beauvericin (BEA), a cyclic hexadepsipeptide produced by the fungus Beauveria bassiana, is known to have anti-cancer, anti-inflammatory, and anti-microbial actions. However, how BEA suppresses macrophage-induced inflammatory responses has not been fully elucidated. In this study, we explored the anti-inflammatory properties of BEA and the underlying molecular mechanisms using lipopolysaccharide (LPS)-treated macrophage-like RAW264.7 cells. Levels of nitric oxide (NO), mRNA levels of transcription factors and the inflammatory genes inducible NO synthase (iNOS) and interleukin (IL)-1, and protein levels of activated intracellular signaling molecules were determined by Griess assay, semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), luciferase reporter gene assay, and immunoblotting analysis...
July 2017: Korean Journal of Physiology & Pharmacology
https://www.readbyqxmd.com/read/28705395/targeted-disruption-of-the-polyketide-synthase-gene-pks15-affects-virulence-against-insects-and-phagocytic-survival-in-the-fungus-beauveria-bassiana
#11
Wachiraporn Toopaang, Suranat Phonghanpot, Juntira Punya, Cheerapha Panyasiri, Kewarin Klamchao, Rudsamee Wasuwan, Chettida Srisuksam, Duangjai Sangsrakru, Chutima Sonthirod, Sithichoke Tangphatsornruang, Morakot Tanticharoen, Alongkorn Amnuaykanjanasin
The reducing clade III polyketide synthase genes, including pks15, are highly conserved among entomopathogenic fungi. To examine the function of pks15, we used targeted disruption to investigate the impact of Beauveria bassiana pks15 on insect pathogenesis. Southern analysis verified that the Δpks15 mutant was disrupted by a single integration of the transformation cassette at the pks15 locus. The Δpks15 mutant had a slight reduction in radial growth, and it produced fewer spores. Our insect bioassays indicated the Δpks15 mutant to be significantly reduced in virulence against beet armyworms compared to wild type (WT), which could be partially accounted for by its markedly decreased ability to survive phagocytosis...
August 2017: Fungal Biology
https://www.readbyqxmd.com/read/28667709/relationship-between-expression-level-of-hygromycin-b-resistant-gene-and-agrobacterium-tumefaciens-mediated-transformation-efficiency-in-beauveria-bassiana-jef-007
#12
Y S Nai, M R Lee, S Kim, S J Lee, J C Kim, Y T Yang, J S Kim
AIMS: Agrobacterium tumefaciens-mediated transformation (AtMT) is an effective method for generation of entomopathogenic Beauveria bassiana transformants. However, some strains grow on the selective medium containing hygromycin B (HygB), which reduces the selection efficiency of the putative transformants. In this work, a relationship between HygB resistance gene promoter and AtMT efficiency was investigated to improve the transformant selection. METHODS AND RESULTS: Ten B...
July 1, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28557308/discovery-of-a-new-intravacuolar-protein-required-for-the-autophagy-development-and-virulence-of-beauveria-bassiana
#13
Zhen-Jian Chu, Huan-Huan Sun, Xiao-Guan Zhu, Sheng-Hua Ying, Ming-Guang Feng
High proportions of hypothetical proteins exist in genomic databases of fungi, including putative secretory proteins (PSPs) likely involved in fungal invasion and virulence. Here we characterize one of many PSPs revealed in the previous transcriptome of Beauveria bassiana (a fungal insect pathogen) infecting a global lepidopteran pest and name it vacuole-localized protein 4 (VLP4) because this small, domain-lacking protein (22.96 kDa) was specifically localized in the vacuoles of hyphal cells. Deletion of VLP4 resulted in repression of almost all genes acting in autophagy and central development pathways...
May 30, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28552321/vital-role-for-cyclophilin-b-cypb-in-asexual-development-dimorphic-transition-and-virulence-of-beauveria-bassiana
#14
Zhen-Jian Chu, Huan-Huan Sun, Sheng-Hua Ying, Ming-Guang Feng
Cyclophilin B (CypB) was previously revealed as one of many putative secretory proteins in the transcriptome of Beauveria bassiana infection to a lepidopteran pest. Here we show a main localization of CypB in hyphal cell walls and septa and its essential role in the in vitro and in vivo asexual cycles of the fungal insect pathogen. Deletion of cypB reduced colony growth by 16-42% on two rich media and 30 scant media with different carbon or nitrogen sources. The deletion mutant suffered a delayed conidiation on a standard medium and a final 47% reduction in conidial yield, accompanied with drastic transcript depression of several key genes required for conidiation and conidial maturation...
August 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28545959/serpin-14-negatively-regulates-prophenoloxidase-activation-and-expression-of-antimicrobial-peptides-in-chinese-oak-silkworm-antheraea-pernyi
#15
Saima Kausar, Muhammad Nadeem Abbas, Cen Qian, Baojian Zhu, Yu Sun, Yuxuan Sun, Lei Wang, Guoqing Wei, Iram Maqsood, Chao-Liang Liu
Genes encoding proteins of serpins superfamily are widely distributed in invertebrates. In insects, serpins play important roles in regulating immune responses and other physiological processes. Here, we report the cloning and characterization of cDNA of Apserpin-14 from Chinese oak silkworm (Antheraea pernyi). The Apserpin-14 gene contains 1206 bp open reading frame, encoding a predicted 401 amino acid residue protein. We expressed the recombinant Apserpin-14 protein in Escherichia coli and then purified protein was used to prepare rabbit anti-Apserpin-14 polyclonal antibodies...
May 22, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28544681/tenebrio-molitor-gram-negative-binding-protein-3-tmgnbp3-is-essential-for-inducing-downstream-antifungal-tenecin-1-gene-expression-against-infection-with-beauveria-bassiana-jef-007
#16
Yi-Ting Yang, Mi Rong Lee, Se Jin Lee, Sihyeon Kim, Yu-Shin Nai, Jae Su Kim
The Toll signaling pathway is responsible for defense against both Gram-positive bacteria and fungi. Gram-negative binding protein 3 (GNBP3) has a strong affinity for the fungal cell wall component, β-1,3-glucan, which can activate the prophenoloxidase (proPO) cascade and induce the Toll signaling pathway. Myeloid differentiation factor 88 (MyD88) is an intracellular adaptor protein involved in the Toll signaling pathway. In this study, we monitored the response of 5 key genes (TmGNBP3, TmMyD88, and Tenecin 1, 2, and 3) in the Toll pathway of the mealworm Tenebrio molitor immune system against the fungus Beauveria bassiana JEF-007 using RT-PCR...
May 23, 2017: Insect Science
https://www.readbyqxmd.com/read/28469160/differential-roles-for-six-p-type-calcium-atpases-in-sustaining-intracellular-ca-2-homeostasis-asexual-cycle-and-environmental-fitness-of-beauveria-bassiana
#17
Jie Wang, Xiao-Guan Zhu, Sheng-Hua Ying, Ming-Guang Feng
A global insight into the roles of multiple P-type calcium ATPase (CA) pumps in sustaining the life of a filamentous fungal pathogen is lacking. Here we elucidated the functions of five CA pumps (Eca1, Spf1 and PmcA/B/C) following previous characterization of Pmr1 in Beauveria bassiana, a fungal insect pathogen. The fungal CA pumps interacted at transcriptional level, at which singular deletions of five CA genes depressed eca1 expression by 76-98% and deletion of spf1 resulted in drastic upregulation of four CA genes by 36-50-fold...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28436337/molecular-cloning-expression-and-characterization-of-e2f-transcription-factor-4-from-antheraea-pernyi
#18
M N Abbas, S Kausar, Y-X Sun, Y Sun, L Wang, C Qian, G-Q Wei, B-J Zhu, C-L Liu
The E2F transcription factor family is distributed widely in eukaryotes and has been well studied among mammals. In the present study, the E2F transcription factor 4 (E2F4) gene was isolated from fat bodies of Antheraea pernyi and sequenced. E2F4 comprised a 795 bp open reading frame encoding a deduced amino acid sequence of 264 amino acid residues. The recombinant protein was expressed in Escherichia coli (Transetta DE3), and anti-E2F4 antibodies were prepared. The deduced amino acid sequence displayed significant homology to an E2F4-like protein from Bombyx mori L...
April 24, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28399190/expression-of-the-insect-metalloproteinase-inhibitor-impi-in-the-fat-body-of-galleria-mellonella-exposed-to-infection-with-beauveria-bassiana
#19
Lidiia Vertyporokh, Iwona Wojda
The inducible metalloproteinase inhibitor (IMPI) discovered in Galleria mellonella is currently the only specific inhibitor of metalloproteinases found in animals. Its role is to inhibit the activity of metalloproteinases secreted by pathogenic organisms as virulence factors to degrade immune-relevant polypeptides of the infected host. This is a good example of an evolutionary arms race between the insect hosts and their natural pathogens. In this report, we analyze the expression of a gene encoding an inducible metalloproteinase inhibitor (IMPI) in fat bodies of the greater wax moth larvae Galleria mellonella infected with an entomopathogenic fungus Beauveria bassiana...
2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/28369437/upregulation-of-a-trypsin-like-serine-protease-gene-in-antheraea-pernyi-lepidoptera-saturniidae-strains-exposed-to-different-pathogens
#20
Ying Sun, Yong Wang, Wei Liu, Jing-Lin Zhou, Jun Zeng, Xiao-Hui Wang, Yi-Ren Jiang, Dong-Hua Li, Li Qin
Antheraea pernyi Guérin-Méneville is used for silk production and as a food resource. Its infection by exogenous pathogens, including microsporidia, fungi, bacteria, and virus, can lead to silkworm diseases, causing major economic losses. A trypsin-like serine protease gene (TLS) was found in A. pernyi transcriptome data resulting from two different infection experiments. The cDNA sequence of ApTLS was 1,020 bp in length and contained an open reading frame of 774 bp encoding a 257-amino acid protein (GenBank KF779933)...
June 1, 2017: Journal of Economic Entomology
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