Read by QxMD icon Read

beauveria bassiana gene

Qing Cai, Sen-Miao Tong, Wei Shao, Sheng-Hua Ying, Ming-Guang Feng
Histone acetyltransferases (HATs) and deacetylases (HDACs) maintain dynamics of lysine acetylation/deacetylation on histones and non-histone substrates involved in gene regulation and cellular events. Hos2 is a class I HDAC that deacetylates unique histone H4-K16 site in yeasts. Here, we report that orthologous Hos2 deacetylates H4-K16 and is also involved in the acetylation of histone H3-K56 and the phosphorylation of histone H2A-S129 and cyclin-dependent kinase 1 CDK1-Y15 in Beauveria bassiana, a filamentous fungal insect pathogen...
March 15, 2018: Cellular Microbiology
Soumya Moonjely, Nemat O Keyhani, Michael J Bidochka
The hyd1/hyd2 hydrophobins are important constituents of the conidial cell wall of the insect pathogenic fungus Beauveria bassiana. This fungus can also form intimate associations with several plant species. Here, we show that inactivation of two Class I hydrophobin genes, hyd1 or hyd2, significantly decreases the interaction of B. bassiana with bean roots. Curiously, the ∆hyd1/∆hyd2 double mutant was less impaired in root association than Δhyd1 or Δhyd2. Loss of hyd genes affected growth rate, conidiation ability and oosporein production...
March 8, 2018: Microbiology
Tilottama Biswas, Gerrit Joop, Charlotte Rafaluk-Mohr
Host-parasite coevolution can influence interactions of the host and parasite with the wider ecological community. One way that this may manifest is in cross-resistance towards other parasites, which has been observed to occur in some host-parasite evolution experiments. In this paper, we test for cross-resistance towards Bacillus thuringiensis and Pseudomonas entomophila in the red flour beetle Tribolium castaneum , which was previously allowed to coevolve with the generalist entomopathogenic fungus Beauveria bassiana ...
February 26, 2018: Insects
Se Jin Lee, Mi Rong Lee, Sihyeon Kim, Jong Cheol Kim, So Eun Park, Tae Young Shin, Jae Su Kim
Beauveria bassiana is an entomopathogenic fungi used in environmentally mindful pest management. Its main active ingredient, conidia, is commercially available as a fungal biopesticide. Many studies of conidia production have focused on how to optimize culture conditions for maximum productivity and stability against unfavorable abiotic factors. However, understanding of how conidiogenesis-related genes provide improved conidial production remains unclear. In this study, we focus on identifying conidiogenesis-related genes in B...
February 14, 2018: Journal of Invertebrate Pathology
Qing Cai, Juan-Juan Wang, Bo Fu, Sheng-Hua Ying, Ming-Guang Feng
Gcn5 is a core histone acetyltransferase that catalyzes histone H3 acetylation on N-terminal lysine residues in yeasts and was reported to catalyze H3K9/K14 acetylation required for activating asexual development in Aspergillus. Here, we report a localization of Gcn5 ortholog in the nucleus and cytoplasm of Beauveria bassiana, a fungal insect pathogen. Deletion of gcn5 led to hypoacetylated H3 at K9/14/18/27 and 97% reduction in conidiation capacity as well as severe defects in colony growth and conidial thermotolerance...
February 7, 2018: Environmental Microbiology
Yibo Huang, Yujiro Higuchi, Takashi Kinoshita, Ai Mitani, Yasunari Eshima, Kaoru Takegawa
Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides. Although many ENGases have been characterized from various organisms, so far no fucose-containing oligosaccharides-specific ENGase has been identified in any organism. Here, we screened soil samples, using dansyl chloride (Dns)-labeled sialylglycan (Dns-SG) as a substrate, and discovered a strain that exhibits ENGase activity in the culture supernatant; this strain, named here as strain HMA12, was identified as a Sphingobacterium species by 16S ribosomal RNA gene analysis...
January 10, 2018: Scientific Reports
Huifang Wang, Zhangjiang He, Linli Luo, Xin Zhao, Zhuoyue Lu, Tingying Luo, Min Li, Yongjun Zhang
The aldo-keto reductases (AKRs) belong to the NADP-dependent oxidoreductase superfamily, which play important roles in various physiological functions in prokaryotic and eukaryotic organisms. However, many AKR superfamily members remain uncharacterized. Here, a downstream target gene of the HOG1 MAPK pathways coding for an aldo-keto reductase, named Bbakr1, was characterized in the insect fungal pathogen, Beauveria bassiana. Bbakr1 expression increased in response to osmotic and salt stressors, and oxidative and heavy metal (chromium) stress...
January 3, 2018: Fungal Genetics and Biology: FG & B
Juan-Juan Wang, Qing Cai, Lei Qiu, Sheng-Hua Ying, Ming-Guang Feng
Histone lysine acetylation orchestrates transcriptional activity essential for diverse cellular events across organisms, but it remains poorly understood how an acetylated lysine affects cellular functions in filamentous fungal pathogens. Here, we show the functions of a histone acetyltransferase that is phylogenetically close to Mst2 in fission yeast and specifically acetylates histone H3K14 in Beauveria bassiana, a fungal insect pathogen widely applied in arthropod pest management. Deletion of mst2 in B. bassiana resulted in moderate growth defects on rich and minimal media, delayed conidiation, and drastic reduction (75%) in conidiation capacity under normal culture conditions...
December 23, 2017: Applied Microbiology and Biotechnology
Zhangjiang He, Xin Zhao, Zhuoyue Lu, Huifang Wang, Pengfei Liu, Fanqin Zeng, Yongjun Zhang
Sensing, responding, and adapting to the surrounding environment are crucial for all living organisms to survive, proliferate, and differentiate in their biological niches. Beauveria bassiana is an economically important insect-pathogenic fungus which is widely used as a biocontrol agent to control a variety of insect pests. The fungal pathogen unavoidably encounters a variety of adverse environmental stresses and defense response from the host insects during application of the fungal agents. However, few are known about the transcription response of the fungus to respond or adapt varied adverse stresses...
December 16, 2017: Journal of Invertebrate Pathology
Anhui Chen, Yulong Wang, Ying Shao, Qiumei Zhou, Shanglong Chen, Yonghua Wu, Hongwei Chen, Enqi Liu
The ascomycete fungus Beauveria bassiana is a natural pathogen of hundreds of insect species and is commercially produced as an environmentally friendly mycoinsecticide. Many genes involved in fungal insecticide infection have been identified but few have been further explored. In this study, we constructed three transcriptomes of B. bassiana at 24, 48 and 72 h post infection of insect pests (BbI) or control (BbC). There were 3148, 3613 and 4922 genes differentially expressed at 24, 48 and 72 h post BbI/BbC infection, respectively...
December 6, 2017: Archives of Microbiology
Chen Chen, Salah Eldein, Xiaosan Zhou, Yu Sun, Jin Gao, Yuxuan Sun, Chaoliang Liu, Lei Wang
The Rab-family GTPases mainly regulate intracellular vesicle transport, and play important roles in the innate immune response in invertebrates. However, the function and signal transduction of Rab proteins in immune reactions remain unclear in silkworms. In this study, we analyzed a Rab-related protein of silkworm Bombyx mori (BmRABRP) by raising antibodies against its bacterially expressed recombinant form. Tissue distribution analysis showed that BmRABRP mRNA and protein were high expressed in the Malpighian tubule and fat body, respectively...
January 2018: Archives of Insect Biochemistry and Physiology
Yuuki Hayakawa, Mitsuki Sawada, Masaki Seki, Patchara Sirasoonthorn, Shotaro Shiga, Katsumi Kamiya, Chieka Minakuchi, Ken Miura
We previously reported that the moderate knockdown of chitin synthase 1 gene of the model beetle Tribolium castaneum impairs the host defense against entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae, which infect host insects via the direct penetration of cuticular integuments (Hayakawa et al, 2017). In this study, we focused on the antifungal roles of laccase2 (Lac2) as well as yellow-e (Y-e) genes, both of which are shown to be important to the establishment of stable cuticular structures in this beetle species...
November 1, 2017: Journal of Invertebrate Pathology
Jin Gao, Yu Sun, Yuxuan Sun, Chen Chen, Saima Kausar, Jiwu Tian, Baojian Zhu, Chaoliang Liu
Cyclic AMP response element binding (CREB) proteins participate in the regulation of many biological processes. However, little is known about their role in immune regulation in the Oak silkworm (Antheraea pernyi). In this study, a CREB gene was identified in A. pernyi and its role in immune regulation was investigated. ApCREB shares conserved signature motifs with other CREB proteins, and includes a typical leucine zipper domain, specific DNA-binding site, nuclear localisation signal (NLS) and cAMP-dependent protein kinase phosphorylation site...
October 24, 2017: Journal of Invertebrate Pathology
Xiao-Guan Zhu, Zhen-Jian Chu, Sheng-Hua Ying, Ming-Guang Feng
Krs is a class II lysyl-tRNA synthetase (KRS) that is involved in cytosolic protein synthesis in budding yeast but functionally has not been explored in filamentous fungi. Previous transcriptomic analysis has revealed that a Krs-coding gene is likely involved in pathogenesis of Beauveria bassiana, a classic insect pathogen as a global source of fungal insecticides. Here, we show that Krs is localized in the cytoplasm of hyphal cells and acts as a substantial virulence factor in B. bassiana. Deletion of krs resulted in 10-h delayed germination, decreased (15 %) thermotolerance, and lowered (46 %) UV-B resistance of aerial conidia despite limited impact on conidiation capacity and slight or inconspicuous influence on radial growth on rich and minimal media with different carbon (10 sugars/polyols) and nitrogen (17 amino acids) sources...
November 2017: Fungal Biology
Sen-Miao Tong, An-Xue Zhang, Chong-Tao Guo, Sheng-Hua Ying, Ming-Guang Feng
The fungal insect pathogen Beauveria bassiana has the blue-light photoreceptor VIVID (VVD) but lacks a pigmentation pattern to trace its light responses. Here we show that the fungal vvd is transcriptionally expressed, and linked to other blue/red photoreceptor genes, in a daylight length-dependent manner. GFP-tagged VVD fusion protein was localized to periphery, cytoplasm and vacuoles of hyphal cells in light/dark (L:D) cycles of 24:0 and 16:8 and aggregated in cytoplasm with shortening daylight until transfer into nuclei in full darkness...
October 2, 2017: Environmental Microbiology
Carla Huarte-Bonnet, Suresh Kumar, Mario C N Saparrat, Juan R Girotti, Marianela Santana, John E Hallsworth, Nicolás Pedrini
Several filamentous fungi are able to concomitantly assimilate both aliphatic and polycyclic aromatic hydrocarbons that are the biogenic by-products of some industrial processes. Cytochrome P450 monooxygenases catalyze the first oxidation reaction for both types of substrate. Among the cytochrome P450 (CYP) genes, the family CYP52 is implicated in the first hydroxylation step in alkane-assimilation processes, while genes belonging to the family CYP53 have been linked with oxidation of aromatic hydrocarbons...
September 23, 2017: Applied Biochemistry and Biotechnology
Sudarshan K Aryal, Derreck Carter-House, Jason E Stajich, Adler R Dillman
We report the isolation and identification of seven bacterial strains and one fungal strain from dead and diseased Scapteriscus borellii mole crickets collected from a golf course in southern California. Using 16S and 18S rRNA gene sequence analysis we identified the microbes as Serratia marcescens (red), S. marcescens (white), S. marcescens (purple), Achromobacter xylosoxidans, Chryseobacterium sp., Ochrobactrum anthropi, Tsukamurella tryosinosolvens, and Beauveria bassiana. We performed a dose response curve for each of these cricket-associated microbial strains (except T...
September 12, 2017: Journal of Invertebrate Pathology
Kai Cen, Bing Li, Yuzhen Lu, Siwei Zhang, Chengshu Wang
The lysin motif (LysM) containing proteins can bind chitin and are ubiquitous in various organisms including fungi. In plant pathogenic fungi, a few LysM proteins have been characterized as effectors to suppress chitin-induced immunity in plant hosts and therefore contribute to fungal virulence. The effector mechanism is still questioned in fungus-animal interactions. In this study, we found that LysM proteins are also present in animal pathogenic fungi and have evolved divergently. The genome of the insect pathogen Beauveria bassiana encodes 12 LysM proteins, and the genes were differentially transcribed by the fungus when grown in different conditions...
September 2017: PLoS Pathogens
Xin-Ling Chu, Wei-Xia Dong, Jin-Li Ding, Ming-Guang Feng, Sheng-Hua Ying
Oxidation tolerance is an important determinant to predict the virulence and biocontrol potential of Beauveria bassiana, a well-known entomopathogenic fungus. As a transcriptional coactivator, multiprotein bridging factor 1 mediates the activity of transcription factor in diverse physiological processes, and its homolog in B. bassiana (BbMBF1) contributes to fungal oxidation tolerance. In this study, the BbMBF1-interactomes under oxidative stress and normal growth condition were deciphered by mass spectrometry integrated with the immunoprecipitation...
September 4, 2017: Current Genetics
Almudena Aranda-Martinez, Miguel Ángel Naranjo Ortiz, Isabel Sofía Abihssira García, Ernesto A Zavala-Gonzalez, Luis Vicente Lopez-Llorca
Chitin is the second most abundant biopolymer after cellulose and virtually unexplored as raw material for bioethanol production. In this paper, we investigate chitosan, the deacetylated form of chitin which is the main component of shellfish waste, as substrate for bioethanol production by fungi. Fungal parasites of invertebrates such as the nematophagous Pochonia chlamydosporia (Pc) or the entomopathogens Beauveria bassiana (Bb) and Metarhizium anisopliae (Ma) are biocontrol agents of plant parasitic nematodes (eg...
November 2017: Microbiological Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"