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beauveria bassiana gene

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https://www.readbyqxmd.com/read/29790944/the-reducing-clade-iib-polyketide-synthase-pks14-acts-as-a-virulence-determinant-of-the-entomopathogenic-fungus-beauveria-bassiana
#1
Chettida Srisuksam, Juntira Punya, Songsak Wattanachaisaereekul, Wachiraporn Toopaang, Supapon Cheevadhanarak, Morakot Tanticharoen, Alongkorn Amnuaykanjanasin
pks14, a reducing clade IIb polyketide synthase gene, is preserved throughout the evolution of entomopathogenic fungi. We examined the functions of pks14 in Beauveria bassiana using targeted gene disruption. pks14 disruption was verified by Southern blot and PCR analyses. The radial growth, cell dry weight and conidial germination of Δpks14 were comparable to that of the wild type. Our sequence and gene expression analyses of the pks14 biosynthetic cluster demonstrated: (i) cotranscription and constitutive expression of nearly all genes of the aforementioned cluster including C2H2 zinc finger transcription regulator gene, but not pks14 and cytochrome P450 gene; (ii) expression of pks14 gene in insect-containing culture condition only; whereby (iii) a KAR9-like gene in direct proximity with pks14 is the only gene showing co-regulation...
May 22, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29760061/methylglucosylation-of-aromatic-amino-and-phenolic-moieties-of-drug-like-biosynthons-by-combinatorial-biosynthesis
#2
Linan Xie, Liwen Zhang, Chen Wang, Xiaojing Wang, Ya-Ming Xu, Hefen Yu, Ping Wu, Shenglan Li, Lida Han, A A Leslie Gunatilaka, Xiaoyi Wei, Min Lin, István Molnár, Yuquan Xu
Glycosylation is a prominent strategy to optimize the pharmacokinetic and pharmacodynamic properties of drug-like small-molecule scaffolds by modulating their solubility, stability, bioavailability, and bioactivity. Glycosyltransferases applicable for "sugarcoating" various small-molecule acceptors have been isolated and characterized from plants and bacteria, but remained cryptic from filamentous fungi until recently, despite the frequent use of some fungi for whole-cell biocatalytic glycosylations...
May 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29704296/rtt109-dependent-histone-h3-k56-acetylation-and-gene-activity-are-essential-for-the-biological-control-potential-of-beauveria-bassiana
#3
Qing Cai, Juan-Juan Wang, Wei Shao, Sheng-Hua Ying, Ming-Guang Feng
BACKGROUND: Rtt109 is a histone acetyltransferase that catalyzes histone H3K56 acetylation required for genomic stability, DNA damage repair and virulence-related gene activity in yeast-like human pathogens but remains functionally unknown in fungal insect pathogens. This study seeks to elucidate catalytic activity of Rtt109 orthologue and its possible role in sustaining biological control potential of Beauveria bassiana, a fungal entomopathogen. RESULTS: Deletion of rtt109 in B...
April 27, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29704043/antioxidant-enzymes-and-their-contributions-to-biological-control-potential-of-fungal-insect-pathogens
#4
REVIEW
Long-Bin Zhang, Ming-Guang Feng
Filamentous fungal insect pathogens represent a source of biological insecticides and acaricides formulated using intact cells, such as conidia or other spores. These mycoinsecticides infect arthropod pests through cuticular penetration. In field application, formulated fungal cells are exposed to environmental stresses, including solar UV irradiation, high temperature, and applied chemical herbicides and fungicides, as well as stress from host immune defenses. These stresses often result in accumulation of toxic reactive oxygen species (ROS), generating oxidative stress to the fungal cells and hence affecting the efficacy and persistency of fungi formulated for pest control...
April 28, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29575704/essential-role-of-rpd3-dependent-lysine-modification-in-the-growth-development-and-virulence-of-beauveria-bassiana
#5
Qing Cai, Zhi-Kang Wang, Wei Shao, Sheng-Hua Ying, Ming-Guang Feng
Rpd3 is a class I histone deacetylase that reverses lysine acetylation thus influencing cellular processes and functions. However, its role in fungal insect pathogens has not been explored yet. Here we show that Rpd3-dependent lysine modification and gene expression orchestrate growth, conidiation and virulence in Beauveria bassiana. Deletion of Rpd3 resulted in severe growth defects on various carbon/nitrogen sources, 97% reduction in conidiation capacity and drastic attenuation in virulence. These phenotypes concurred with differential expression of 1479 proteins and hyperacetylation or hypoacetylation of 2227 lysine residues on 1134 proteins...
March 24, 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29543404/pleiotropic-effects-of-the-histone-deacetylase-hos2-linked-to-h4-k16-deacetylation-h3-k56-acetylation-and-h2a-s129-phosphorylation-in-beauveria-bassiana
#6
Qing Cai, Sen-Miao Tong, Wei Shao, Sheng-Hua Ying, Ming-Guang Feng
Histone acetyltransferases and deacetylases maintain dynamics of lysine acetylation/deacetylation on histones and nonhistone substrates involved in gene regulation and cellular events. Hos2 is a Class I histone deacetylases that deacetylates unique histone H4-K16 site in yeasts. Here, we report that orthologous Hos2 deacetylates H4-K16 and is also involved in the acetylation of histone H3-K56 and the phosphorylation of histone H2A-S129 and cyclin-dependent kinase 1 CDK1-Y15 in Beauveria bassiana, a filamentous fungal insect pathogen...
March 15, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29517481/hydrophobins-contribute-to-root-colonization-and-stress-responses-in-the-rhizosphere-competent-insect-pathogenic-fungus-beauveria-bassiana
#7
Soumya Moonjely, Nemat O Keyhani, Michael J Bidochka
The hyd1/hyd2 hydrophobins are important constituents of the conidial cell wall of the insect pathogenic fungus Beauveria bassiana. This fungus can also form intimate associations with several plant species. Here, we show that inactivation of two Class I hydrophobin genes, hyd1 or hyd2, significantly decreases the interaction of B. bassiana with bean roots. Curiously, the ∆hyd1/∆hyd2 double mutant was less impaired in root association than Δhyd1 or Δhyd2. Loss of hyd genes affected growth rate, conidiation ability and oosporein production...
April 2018: Microbiology
https://www.readbyqxmd.com/read/29495405/cross-resistance-a-consequence-of-bi-partite-host-parasite-coevolution
#8
Tilottama Biswas, Gerrit Joop, Charlotte Rafaluk-Mohr
Host-parasite coevolution can influence interactions of the host and parasite with the wider ecological community. One way that this may manifest is in cross-resistance towards other parasites, which has been observed to occur in some host-parasite evolution experiments. In this paper, we test for cross-resistance towards Bacillus thuringiensis and Pseudomonas entomophila in the red flour beetle Tribolium castaneum , which was previously allowed to coevolve with the generalist entomopathogenic fungus Beauveria bassiana ...
February 26, 2018: Insects
https://www.readbyqxmd.com/read/29453964/conidiogenesis-related-dna-photolyase-gene-in-beauveria-bassiana
#9
Se Jin Lee, Mi Rong Lee, Sihyeon Kim, Jong Cheol Kim, So Eun Park, Tae Young Shin, Jae Su Kim
Beauveria bassiana is an entomopathogenic fungi used in environmentally mindful pest management. Its main active ingredient, conidia, is commercially available as a fungal biopesticide. Many studies of conidia production have focused on how to optimize culture conditions for maximum productivity and stability against unfavorable abiotic factors. However, understanding of how conidiogenesis-related genes provide improved conidial production remains unclear. In this study, we focus on identifying conidiogenesis-related genes in B...
March 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29417710/gcn5-dependent-histone-h3-acetylation-and-gene-activity-is-required-for-the-asexual-development-and-virulence-of-beauveria-bassiana
#10
Qing Cai, Juan-Juan Wang, Bo Fu, Sheng-Hua Ying, Ming-Guang Feng
Gcn5 is a core histone acetyltransferase that catalyzes histone H3 acetylation on N-terminal lysine residues in yeasts and was reported to catalyze H3K9/K14 acetylation required for activating asexual development in Aspergillus. Here, we report a localization of Gcn5 ortholog in the nucleus and cytoplasm of Beauveria bassiana, a fungal insect pathogen. Deletion of gcn5 led to hypoacetylated H3 at K9/14/18/27 and 97% reduction in conidiation capacity as well as severe defects in colony growth and conidial thermotolerance...
April 2018: Environmental Microbiology
https://www.readbyqxmd.com/read/29321565/characterization-of-novel-endo-%C3%AE-n-acetylglucosaminidases-from-sphingobacterium-species-beauveria-bassiana-and-cordyceps-militaris-that-specifically-hydrolyze-fucose-containing-oligosaccharides-and-human-igg
#11
Yibo Huang, Yujiro Higuchi, Takashi Kinoshita, Ai Mitani, Yasunari Eshima, Kaoru Takegawa
Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides. Although many ENGases have been characterized from various organisms, so far no fucose-containing oligosaccharides-specific ENGase has been identified in any organism. Here, we screened soil samples, using dansyl chloride (Dns)-labeled sialylglycan (Dns-SG) as a substrate, and discovered a strain that exhibits ENGase activity in the culture supernatant; this strain, named here as strain HMA12, was identified as a Sphingobacterium species by 16S ribosomal RNA gene analysis...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29305969/an-aldo-keto-reductase-bbakr1-is-involved-in-stress-response-and-detoxification-of-heavy-metal-chromium-but-not-required-for-virulence-in-the-insect-fungal-pathogen-beauveria-bassiana
#12
Huifang Wang, Zhangjiang He, Linli Luo, Xin Zhao, Zhuoyue Lu, Tingying Luo, Min Li, Yongjun Zhang
The aldo-keto reductases (AKRs) belong to the NADP-dependent oxidoreductase superfamily, which play important roles in various physiological functions in prokaryotic and eukaryotic organisms. However, many AKR superfamily members remain uncharacterized. Here, a downstream target gene of the HOG1 MAPK pathways coding for an aldo-keto reductase, named Bbakr1, was characterized in the insect fungal pathogen, Beauveria bassiana. Bbakr1 expression increased in response to osmotic and salt stressors, and oxidative and heavy metal (chromium) stress...
February 2018: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29275430/the-histone-acetyltransferase-mst2-sustains-the-biological-control-potential-of-a-fungal-insect-pathogen-through-transcriptional-regulation
#13
Juan-Juan Wang, Qing Cai, Lei Qiu, Sheng-Hua Ying, Ming-Guang Feng
Histone lysine acetylation orchestrates transcriptional activity essential for diverse cellular events across organisms, but it remains poorly understood how an acetylated lysine affects cellular functions in filamentous fungal pathogens. Here, we show the functions of a histone acetyltransferase that is phylogenetically close to Mst2 in fission yeast and specifically acetylates histone H3K14 in Beauveria bassiana, a fungal insect pathogen widely applied in arthropod pest management. Deletion of mst2 in B. bassiana resulted in moderate growth defects on rich and minimal media, delayed conidiation, and drastic reduction (75%) in conidiation capacity under normal culture conditions...
February 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29258843/comparative-transcriptome-and-gene-co-expression-network-analysis-reveal-genes-and-signaling-pathways-adaptively-responsive-to-varied-adverse-stresses-in-the-insect-fungal-pathogen-beauveria-bassiana
#14
Zhangjiang He, Xin Zhao, Zhuoyue Lu, Huifang Wang, Pengfei Liu, Fanqin Zeng, Yongjun Zhang
Sensing, responding, and adapting to the surrounding environment are crucial for all living organisms to survive, proliferate, and differentiate in their biological niches. Beauveria bassiana is an economically important insect-pathogenic fungus which is widely used as a biocontrol agent to control a variety of insect pests. The fungal pathogen unavoidably encounters a variety of adverse environmental stresses and defense response from the host insects during application of the fungal agents. However, few are known about the transcription response of the fungus to respond or adapt varied adverse stresses...
January 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29214339/genes-involved-in-beauveria-bassiana-infection-to-galleria-mellonella
#15
Anhui Chen, Yulong Wang, Ying Shao, Qiumei Zhou, Shanglong Chen, Yonghua Wu, Hongwei Chen, Enqi Liu
The ascomycete fungus Beauveria bassiana is a natural pathogen of hundreds of insect species and is commercially produced as an environmentally friendly mycoinsecticide. Many genes involved in fungal insecticide infection have been identified but few have been further explored. In this study, we constructed three transcriptomes of B. bassiana at 24, 48 and 72 h post infection of insect pests (BbI) or control (BbC). There were 3148, 3613 and 4922 genes differentially expressed at 24, 48 and 72 h post BbI/BbC infection, respectively...
December 6, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/29193252/immune-function-of-a-rab-related-protein-by-modulating-the-jak-stat-signaling-pathway-in-the-silkworm-bombyx-mori
#16
Chen Chen, Salah Eldein, Xiaosan Zhou, Yu Sun, Jin Gao, Yuxuan Sun, Chaoliang Liu, Lei Wang
The Rab-family GTPases mainly regulate intracellular vesicle transport, and play important roles in the innate immune response in invertebrates. However, the function and signal transduction of Rab proteins in immune reactions remain unclear in silkworms. In this study, we analyzed a Rab-related protein of silkworm Bombyx mori (BmRABRP) by raising antibodies against its bacterially expressed recombinant form. Tissue distribution analysis showed that BmRABRP mRNA and protein were high expressed in the Malpighian tubule and fat body, respectively...
January 2018: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/29102780/involvement-of-laccase2-and-yellow-e-genes-in-antifungal-host-defense-of-the-model-beetle-tribolium-castaneum
#17
Yuuki Hayakawa, Mitsuki Sawada, Masaki Seki, Patchara Sirasoonthorn, Shotaro Shiga, Katsumi Kamiya, Chieka Minakuchi, Ken Miura
We previously reported that the moderate knockdown of chitin synthase 1 gene of the model beetle Tribolium castaneum impairs the host defense against entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae, which infect host insects via the direct penetration of cuticular integuments (Hayakawa et al., 2017). In this study, we focused on the antifungal roles of laccase2 (Lac2) as well as yellow-e (Y-e) genes, both of which are shown to be important to the establishment of stable cuticular structures in this beetle species...
January 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29079530/identification-and-function-of-camp-response-element-binding-protein-in-oak-silkworm-antheraea-pernyi
#18
Jin Gao, Yu Sun, Yuxuan Sun, Chen Chen, Saima Kausar, Jiwu Tian, Baojian Zhu, Chaoliang Liu
Cyclic AMP response element binding (CREB) proteins participate in the regulation of many biological processes. However, little is known about their role in immune regulation in the Oak silkworm (Antheraea pernyi). In this study, a CREB gene was identified in A. pernyi and its role in immune regulation was investigated. ApCREB shares conserved signature motifs with other CREB proteins, and includes a typical leucine zipper domain, specific DNA-binding site, nuclear localisation signal (NLS) and cAMP-dependent protein kinase phosphorylation site...
January 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29029702/lysyl-trna-synthetase-krs-acts-a-virulence-factor-of-beauveria-bassiana-by-its-vital-role-in-conidial-germination-and-dimorphic-transition
#19
Xiao-Guan Zhu, Zhen-Jian Chu, Sheng-Hua Ying, Ming-Guang Feng
Krs is a class II lysyl-tRNA synthetase (KRS) that is involved in cytosolic protein synthesis in budding yeast but functionally has not been explored in filamentous fungi. Previous transcriptomic analysis has revealed that a Krs-coding gene is likely involved in pathogenesis of Beauveria bassiana, a classic insect pathogen as a global source of fungal insecticides. Here, we show that Krs is localized in the cytoplasm of hyphal cells and acts as a substantial virulence factor in B. bassiana. Deletion of krs resulted in 10-h delayed germination, decreased (15 %) thermotolerance, and lowered (46 %) UV-B resistance of aerial conidia despite limited impact on conidiation capacity and slight or inconspicuous influence on radial growth on rich and minimal media with different carbon (10 sugars/polyols) and nitrogen (17 amino acids) sources...
November 2017: Fungal Biology
https://www.readbyqxmd.com/read/28967173/daylight-length-dependent-translocation-of-vivid-photoreceptor-in-cells-and-its-essential-role-in-conidiation-and-virulence-of-beauveria-bassiana
#20
Sen-Miao Tong, An-Xue Zhang, Chong-Tao Guo, Sheng-Hua Ying, Ming-Guang Feng
The fungal insect pathogen Beauveria bassiana has the blue-light photoreceptor VIVID (VVD) but lacks a pigmentation pattern to trace its light responses. Here, we show that the fungal vvd is transcriptionally expressed, and linked to other blue/red photoreceptor genes, in a daylight length-dependent manner. GFP-tagged VVD fusion protein was localized to periphery, cytoplasm and vacuoles of hyphal cells in light/dark (L:D) cycles of 24:0 and 16:8 and aggregated in cytoplasm with shortening daylight until transfer into nuclei in full darkness...
January 2018: Environmental Microbiology
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