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https://www.readbyqxmd.com/read/29224250/multiple-autologous-bone-marrow-derived-cd271-mesenchymal-stem-cell-transplantation-overcomes-drug-resistant-epilepsy-in-children
#1
Olga Milczarek, Danuta Jarocha, Anna Starowicz-Filip, Stanislaw Kwiatkowski, Bogna Badyra, Marcin Majka
There is a need among patients suffering from drug-resistant epilepsy (DRE) for more efficient and less toxic treatments. The objective of the present study was to assess the safety, feasibility, and potential efficacy of autologous bone marrow cell transplantation in pediatric patients with DRE. Two females and two males (11 months to 6 years) were enrolled and underwent a combined therapy consisting of autologous bone marrow nucleated cells (BMNCs) transplantation (intrathecal: 0.5 × 109 ; intravenous: 0...
December 10, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29224121/high-definition-infrared-thermography-of-ice-nucleation-and-propagation-in-wheat-under-natural-frost-conditions-and-controlled-freezing
#2
David P Livingston, Tan D Tuong, J Paul Murphy, Lawrence V Gusta, Ian Willick, Micheal E Wisniewski
An extremely high resolution infrared camera demonstrated various freezing events in wheat under natural conditions. Many of those events shed light on years of misunderstanding regarding freezing in small grains. Infrared thermography has enhanced our knowledge of ice nucleation and propagation in plants through visualization of the freezing process. The majority of infrared analyses have been conducted under controlled conditions and often on individual organs instead of whole plants. In the present study, high-definition (1280 × 720 pixel resolution) infrared thermography was used under natural conditions to visualize the freezing process of wheat plants during freezing events in 2016 and 2017...
December 9, 2017: Planta
https://www.readbyqxmd.com/read/29224082/generating-electric-current-by-bioartificial-photosynthesis
#3
Babu Halan, Jenny Tschörtner, Andreas Schmid
Abundant solar energy can be a sustainable source of energy. This chapter highlights recent advancements, challenges, and future scenarios in bioartificial photosynthesis, which is a new subset of bioelectrochemical systems (BESs) and technologies. BES technologies exploit the catalytic interactions between biological moieties and electrodes. At the nexus of BES and photovoltaics, this review focuses on light-harvesting technologies based on bioartificial photosynthesis. Such technologies are promising because electrical energy is generated from sunlight and water without the need for additional organic feedstock...
December 10, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/29224067/multiplexed-targeted-sequencing-for-oxford-nanopore-minion-a-detailed-library-preparation-procedure
#4
Timokratis Karamitros, Gkikas Magiorkinis
MinION is a small form factor sequencer recently retailed by Oxford Nanopore technologies. This lighter-sized USB3.0-interfaced device uses innovative nanotechnology to generate extra-long reads from libraries prepared using only standard molecular biology lab equipment. The flexibility and the portability of the platform makes it ideal for point-of-interest and real-time surveillance applications. However, MinION's limited capacity is not enough for the study of specific targets within larger genomes. Apart from just PCR-amplifying regions of interest, the capture of long reads spanning the edges of known-unknown genomic regions is of great importance for structural studies, such as the identification of mobile elements' integrations sites, bridging over low complexity repetitive regions etc...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29224039/state-of-the-art-lasers-and-light-treatments-for-vascular-lesions-from-red-faces-to-vascular-malformations
#5
Manuel Valdebran, Brent Martin, Kristen M Kelly
Notable milestones in the treatment of vascular lesions have been achieved over the past century. Many cutaneous vascular lesions can be successfully treated with lightbased devices. In this review, we will discuss the treatment of port-wine birthmarks, lymphatic malformations, infantile hemangiomas, rosacea, venous lakes, pyogenic granulomas, cherry angiomas, and angiofibromas using lasers, total reflection amplification of spontaneous emission of radiation, intense pulsed light, and photodynamic therapy. In addition, for several of these diagnoses, we will review medical therapies that can be combined with light-based devices to provide enhanced results...
December 2017: Seminars in Cutaneous Medicine and Surgery
https://www.readbyqxmd.com/read/29224036/laser-advances-in-the-treatment-of-burn-and-traumatic-scars
#6
Shilpi Khetarpal, Urvashi Kaw, Jeffrey S Dover, Kenneth A Arndt
The realm of scar management is constantly changing. Many factors need to be considered when developing a comprehensive treatment plan, including the nature of the scar and the patient. Scar characteristics can be divided by color, scar type and thickness, and body location. Topical and intralesional agents and light- and laserbased treatments can be used to revitalize and restore damaged skin in atrophic and hypertrophic scars. The most commonly used lasers are the pulsed-dye laser (PDL) and fractional lasers...
December 2017: Seminars in Cutaneous Medicine and Surgery
https://www.readbyqxmd.com/read/29224029/lasers-lights-and-energy-devices-state-of-the-art-introduction
#7
Omer Ibrahim, Jeffrey S Dover, Kenneth A Arndt
No abstract text is available yet for this article.
December 2017: Seminars in Cutaneous Medicine and Surgery
https://www.readbyqxmd.com/read/29224027/a-clinical-scoring-system-for-diagnosis-of-ocular-demodicosis
#8
Oktay Alver, Sertaç Argun Kıvanç, Berna Akova Budak, Nazmiye Ülkü Tüzemen, Beyza Ener, Ahmet Tuncer Özmen
BACKGROUND Demodex may cause chronic and refractory blepharitis with associated ocular surface problems, and its diagnosis and treatment can be quite challenging. In this study, our aim was to assess the efficacy of tea tree oil in Demodex treatment on caucasian patients in an industrialized region of Turkey, and to develop a systematic scoring system for extremely accurate diagnosis in the absence of advanced facilities. MATERIAL AND METHODS Charts of 412 patients with blepharitis were reviewed. A group of 39 out of 412 cases were identified as chronic and treatment-refractory, and therefore were enrolled in this study...
December 10, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29224009/itraq-based-proteomics-analysis-of-acute-lung-injury-induced-by-oleic-acid-in-mice
#9
Xiaotao Xu, Qingwei Zhu, Rong Zhang, Yan Wang, Fangfang Niu, Wenying Wang, Dawei Sun, Aizhong Wang
BACKGROUND/AIMS: This study was conducted to investigate the relationship between differentially expressed proteins (DEPs) and the pathogenesis of oleic acid (OA)-induced acute lung injury (ALI) in mice. METHODS: Eight-week-old male C57BL/6 mice were injected with OA through the tail vein and sacrificed 6 hours after OA administration to identify protein expression levels in lung tissue using isobaric tags for relative and absolute quantification (iTRAQ) technology...
December 8, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29224005/evidence-of-osteoclastic-activity-in-the-human-temporal-bone
#10
Takefumi Kamakura, Joseph B Nadol
Bone remodeling within the otic capsule has been reported to be inhibited especially at or near the cochlea, except under some pathological conditions such as otosclerosis, Paget's disease, or mastoiditis, when bone remodeling can occur. Microcavitations found in periosteal and endosteal layers of human temporal bone specimens without otosclerosis, Paget's disease, or inflammation as reported in the current study are consistent with osteoclastic bone resorption. Thirty-three temporal bones from 33 patients were prepared for light microscopy and classified into 4 groups: histologically proven dehiscence of the superior semicircular canal (SSCD) (n = 3, group 1), age 20 years or younger (n = 10, group 2), age 90 years or older and with otosclerosis (n = 10, group 3), and age 90 years or older without otosclerosis (n = 10, group 4)...
December 9, 2017: Audiology & Neuro-otology
https://www.readbyqxmd.com/read/29223930/frequency-selectivity-in-macaque-monkeys-measured-using-a-notched-noise-method
#11
Jane A Burton, Margit E Dylla, Ramnarayan Ramachandran
The auditory system is thought to process complex sounds through overlapping bandpass filters. Frequency selectivity as estimated by auditory filters has been well quantified in humans and other mammalian species using behavioral and physiological methodologies, but little work has been done to examine frequency selectivity in nonhuman primates. In particular, knowledge of macaque frequency selectivity would help address the recent controversy over the sharpness of cochlear tuning in humans relative to other animal species...
November 28, 2017: Hearing Research
https://www.readbyqxmd.com/read/29223906/comparison-of-real-world-and-certification-emission-rates-for-light-duty-gasoline-vehicles
#12
Tanzila Khan, H Christopher Frey
U.S. light duty vehicles are subject to the U.S. Environmental Protection Agency (EPA) emission standards. Emission compliance is determined by certification testing of selected emissions from representative vehicles on standard driving cycles using chassis dynamometers. Test results are also used in many emission inventories. The dynamometer based emission rates are adjusted to provide the certification levels (CL), which must be lower than the standards for compliance. Although standard driving cycles are based on specific observations of real-world driving, they are not necessarily real-world representative...
December 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29223818/application-of-airborne-photogrammetry-for-the-visualisation-and-assessment-of-contamination-migration-arising-from-a-fukushima-waste-storage-facility
#13
D T Connor, P G Martin, N T Smith, L Payne, C Hutton, O D Payton, Y Yamashiki, T B Scott
Airborne systems such as lightweight and highly portable unmanned aerial vehicles (UAVs) are becoming increasingly widespread in both academia and industry - with an ever-increasing range of applications, including (but not limited to), air quality sampling, wildlife monitoring and land-use mapping. In this work, high-resolution airborne photogrammetry obtained using a multi-rotor system operating at low survey altitudes, is combined with ground-based radiation mapping data acquired at an interim storage facility for wastes removed as part of the large-scale Fukushima clean-up program...
December 7, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29223790/temporal-and-bi-directional-associations-between-sleep-duration-and-physical-activity-sedentary-time-in-children-an-international-comparison
#14
Yingyi Lin, Mark S Tremblay, Peter T Katzmarzyk, Mikael Fogelholm, Gang Hu, Estelle V Lambert, Carol Maher, Jose Maia, Timothy Olds, Olga L Sarmiento, Martyn Standage, Catrine Tudor-Locke, Jean-Philippe Chaput
The purpose of this multinational and cross-sectional study was to investigate whether nighttime sleep duration was associated with physical activity (PA) and sedentary time (SED) the following day, whether daytime PA/SED were associated with sleep duration the subsequent night, and whether the associations were modified by sex and study sites. Data from 5779 children aged 9-11years were analyzed. A waist-worn Actigraph GT3X+ accelerometer was used to assess children's 24-h movement behaviours for 7days, i...
December 6, 2017: Preventive Medicine
https://www.readbyqxmd.com/read/29223755/in-situ-biomineralization-by-silkworm-feeding-with-ion-precursors-for-the-improved-mechanical-properties-of-silk-fiber
#15
Zhenhu Guo, Wensheng Xie, Qin Gao, Dan Wang, Fei Gao, Shengrong Li, Lingyun Zhao
Possessing excellent biocompatibility, biodegradability and good reactive activity, silk fiber has been attracting great attention in biomedicine including surgical suture, drug delivery and tissue engineering. So far, several protocols have been developed to further improve the mechanical properties of the silk fiber. In current study, a novel in suit biomineralization strategy was developed to produce nano-hydroxyapatite (HA) strengthened silk fiber based on the natural alkaline condition in the body of silkworm by feeding the silkworm with ion precursors of Ca2+ and PO43- ions...
December 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29223737/synthesis-and-characterization-of-small-sized-gold-nanoparticles-coated-by-bovine-serum-albumin-bsa-for-cancer-photothermal-therapy
#16
Selma M H Al-Jawad, Ali A Taha, Mohammad M F Al-Habosiy, Lamyaa F A Al-Barram
In the present study, small gold nanoparticles <5nm coated with natural protein Bovine Serum Albumin (BSA) was synthesized and characterized using UV-Vis spectrophotometer, Dynamic Light Scattering (DLS), Transmission Electron Microscopy (TEM), zeta potential and scanning electron microscopy (SEM). Three types of cancer cell lines; Rhabdomyosarcoma (RD), Murine fibroblast (L20B) and RAW 264.7 monocyte-macrophage (MQ) were tested and treated by photothermal strategy, in vitro, by conjugating BSA-AuNPs complex of (0...
December 6, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29223712/correlated-colour-temperature-of-morning-light-influences-alertness-and-body-temperature
#17
Marije Te Kulve, Luc Schlangen, Lisje Schellen, Jan L Souman, Wouter van Marken Lichtenbelt
Though several studies have reported human alertness to be affected by the intensity and spectral composition of ambient light, the mechanism behind this effect is still largely unclear, especially for daytime exposure. Alerting effects of nocturnal light exposure are correlated with melatonin suppression, but melatonin levels are generally low during the day. The aim of this study was to explore the alerting effect of light in the morning for different correlated colour temperature (CCT) values, as well as its interaction with ambient temperature...
December 6, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/29223650/dry-eye-following-cataract-surgery-the-effect-of-light-exposure-using-an-in-vitro-model
#18
Tugce Ipek, Mariana Petronela Hanga, Andreas Hartwig, James Wolffsohn, Clare O'Donnell
PURPOSE: Cataract surgery can lead to the temporary development or worsening of dry eye symptoms. Contributing factors may include corneal incisions, agents used before, during or after surgery, light from the operating microscope, disruption of ocular surface tissues and inflammatory processes. The purpose of this study was to observe the effect of light exposure on conjunctival fibroblasts in order to determine whether light has an effect on wound healing closure, assuming that operating microscopes might have an effect on the ocular surface...
December 6, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/29223581/toward-new-forms-of-meal-sharing-collective-habits-and-personal-diets
#19
Estelle Masson, Sandrine Bubendorff, Christèle Fraïssé
This article sheds light on the fact that the commensality remains a fundamental aspect of eating in French culture. However, at the same time, the expansion of the individualization and medicalization of the act of eating during the latest decades impacted the social representations of food. We will first place dietary practices into a general context in which the relationship to food tends to be individualised and in which health-related issues remain an important aspect of the discourse about food on internet...
December 6, 2017: Appetite
https://www.readbyqxmd.com/read/29223553/lipopolysaccharide-induces-autophagy-by-targeting-the-ampk-mtor-pathway-in-human-nasal-epithelial-cells
#20
Xue-Hai Wang, Zhong-Hua Zhang, Xiao-Lan Cai, Ping Ye, Xin Feng, Ting-Ting Liu, Xue-Zhong Li
Chronic rhinosinusitis (CRS) is a well-known disease encountered in the department of otorhinolaryngology, yet little is known about its pathogenesis. Autophagy, a lysosome-dependent degradation process, has been reported to be involved in the process of many chronic inflammatory diseases. Here we tried to evaluate the function of autophagy in CRS as well as explore the related mechanisms. We first stained light chain 3B (LC3B) with immunohistochemistry in uncinate tissues (UT) from patients with and without CRS and found that its expression was up-regulated in CRS patients...
December 6, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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