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https://www.readbyqxmd.com/read/28228337/minimizing-the-mandibular-advancement-in-an-oral-appliance-for-the-treatment-of-obstructive-sleep-apnea
#1
Eduardo Anitua, Joaquín Durán-Cantolla, Gabriela Zamora Almeida, Mohammad Hamdan Alkhraisat
OBJECTIVE: In the treatment of obstructive sleep apnea (OSA) with an oral appliance (OA), there is no gold standard method to fine-tune the mandibular advancement. This study aimed to analyze the effect of gradual increment of mandibular advancement on the evolution of the apnea-hypopnea index (AHI). METHODS: OSA patients were recruited from a sleep unit. All treatments started with an oral appliance without mandibular advancement. After two weeks, the AHI was assessed with respiratory polygraphy...
January 29, 2017: Sleep Medicine
https://www.readbyqxmd.com/read/28196113/chasm-in-primary-care-provision-in-a-universal-health-system-findings-from-a-nationally-representative-survey-of-health-facilities-in-malaysia
#2
Huy Ming Lim, Sheamini Sivasampu, Ee Ming Khoo, Kamaliah Mohamad Noh
BACKGROUND: Malaysia has achieved universal health coverage since 1980s through the expansion of direct public provision, particularly in rural areas. However, no systematic examination of the rural-urban distribution of primary care services and resources has been conducted to date for policy impact evaluation. METHODS: We conducted a national cross-sectional survey of 316 public and 597 private primary care clinics, selected through proportionate stratified random sampling, from June 2011 through February 2012...
2017: PloS One
https://www.readbyqxmd.com/read/28188524/hydrophilic-strong-anion-exchange-hsax-chromatography-enables-deep-fractionation-of-tissue-proteomes
#3
Benjamin Ruprecht, Dongxue Wang, Riccardo Zenezini Chiozzi, Li-Hua Li, Hannes Hahne, Bernhard Kuster
The bottom-up proteomic analysis of cell line and tissue samples to a depth > 10,000 proteins still represents a considerable challenge because of the sheer number of peptides generated by proteolytic digestions and the high dynamic range of protein expression. As a result, comprehensive protein coverage requires multidimensional peptide separation. Recently, off-line hydrophilic strong cation exchange (hSAX) chromatography has proven its merits for high resolution separation of peptides due to its high degree of orthogonality to reversed-phase liquid chromatography...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28188522/optimized-enrichment-of-phosphoproteomes-by-fe-imac-column-chromatography
#4
Benjamin Ruprecht, Heiner Koch, Petra Domasinska, Martin Frejno, Bernhard Kuster, Simone Lemeer
Phosphorylation is among the most important post-translational modifications of proteins and has numerous regulatory functions across all domains of life. However, phosphorylation is often substoichiometric, requiring selective and sensitive methods to enrich phosphorylated peptides from complex cellular digests. Various methods have been devised for this purpose and we have recently described a Fe-IMAC HPLC column chromatography setup which is capable of comprehensive, reproducible, and selective enrichment of phosphopeptides out of complex peptide mixtures...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28178955/the-management-of-the-faeces-passed-by-under-five-children-an-exploratory-cross-sectional-research-in-an-urban-community-in-southwest-nigeria
#5
Olufemi Oludare Aluko, Olusegun Temitope Afolabi, Emmanuel Abiodun Olaoye, Adeyinka Daniel Adebayo, Seun Oladele Oyetola, Oluwaseun Olamide Abegunde
BACKGROUND: Safe management of faeces (SMoF) and environmental contamination by faecal pathogens have been extensively researched although the SMoF in under-five children has been perennially neglected perhaps due to the misconception that it is harmless. This research, therefore, studied the situation, to determine the magnitude and dimensions of the problem aimed at making policy level stakeholders aware of child faeces management systems and so, inform evidence-based implementation of child and health-related programmes in Nigeria...
February 8, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28177727/associations-between-physical-activity-screen-time-and-fitness-among-6-to-10-year-old-children-living-in-edmonton-canada
#6
Morgan Potter, John C Spence, Normand G Boulé, Jodie A Stearns, Valerie Carson
The objectives of this study were to describe fitness levels, examine associations between physical activity (PA), screen time (ST), and fitness, and examine sex moderating effects in a sample of children. Participants were 649 children (7.8±0.6years; 52.4% female) from Edmonton, Canada. Hours/week of PA and ST were parent-reported. PA was also objectively measured with pedometers and expressed in increments of 1000 steps/day. Fitness components (i.e., vertical jump, sit-and-reach, waist circumference, grip strength, predicted maximal oxygen consumption (VO₂ max), push-ups, partial curl-ups, overall fitness) were measured according to the Canadian PA, Fitness and Lifestyle Approach protocols and expressed as z-scores or low/high fitness...
January 3, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28177357/technical-note-avoiding-the-direct-inversion-of-the-numerator-relationship-matrix-for-genotyped-animals-in-single-step-genomic-best-linear-unbiased-prediction-solved-with-the-preconditioned-conjugate-gradient
#7
Y Masuda, I Misztal, A Legarra, S Tsuruta, D A L Lourenco, B O Fragomeni, I Aguilar
This paper evaluates an efficient implementation to multiply the inverse of a numerator relationship matrix for genotyped animals () by a vector (). The computation is required for solving mixed model equations in single-step genomic BLUP (ssGBLUP) with the preconditioned conjugate gradient (PCG). The inverse can be decomposed into sparse matrices that are blocks of the sparse inverse of a numerator relationship matrix () including genotyped animals and their ancestors. The elements of were rapidly calculated with the Henderson's rule and stored as sparse matrices in memory...
January 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28138134/time-spent-in-sedentary-posture-is-associated-with-waist-circumference-and-cardiovascular-risk
#8
W W Tigbe, M H Granat, N Sattar, M E J Lean
BACKGROUND: The relationship between metabolic risk and time spent sitting, standing and stepping has not been well established. The present study aimed to determine associations of objectively measured time spent siting, standing and stepping, with coronary heart disease (CHD) risk. METHODS: A cross-sectional study of healthy non-smoking Glasgow postal workers, n=111 (55 office-workers, 5 women, and 56 walking/delivery-workers, 10 women), who wore activPAL physical activity monitors for seven days...
January 31, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28128213/high-throughput-automated-microfluidic-sample-preparation-for-accurate-microbial-genomics
#9
Soohong Kim, Joachim De Jonghe, Anthony B Kulesa, David Feldman, Tommi Vatanen, Roby P Bhattacharyya, Brittany Berdy, James Gomez, Jill Nolan, Slava Epstein, Paul C Blainey
Low-cost shotgun DNA sequencing is transforming the microbial sciences. Sequencing instruments are so effective that sample preparation is now the key limiting factor. Here, we introduce a microfluidic sample preparation platform that integrates the key steps in cells to sequence library sample preparation for up to 96 samples and reduces DNA input requirements 100-fold while maintaining or improving data quality. The general-purpose microarchitecture we demonstrate supports workflows with arbitrary numbers of reaction and clean-up or capture steps...
January 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/28127346/trypanosoma-cruzi-evaluation-of-pcr-as-a-laboratory-tool-to-follow-up-the-evolution-of-parasite-load
#10
Fabiana Nabarro Ferraz, Denise Lessa Aleixo, Ana Paula Gruendling, Mônica Lúcia Gomes, Max Jean de Ornelas Toledo, Silvana Marques DE Araújo
BACKGROUND: The study evaluated qualitative PCR, primers 121-122 as a tool to follow up evolution parasite load of Trypanosoma cruzi. METHODS: The study was conducted at the State University of Maringa, in 2015. Step 1, dilutions 1/10 were performed from T. cruzi-Y strain to obtain preparations of 50,000-0.05 parasites/mL from which DNA were extracted, quantified, and amplified. Step 2, the extracted DNA in the dilutions 5-0.05 parasites/mL was re-diluted 1/10, 1/100, 1/1000, quantified, and amplified...
July 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28126900/loss-less-nano-fractionator-for-high-sensitivity-high-coverage-proteomics
#11
Nils A Kulak, Philipp E Geyer, Matthias Mann
Recent advances in mass spectrometry (MS)-based proteomics now allow very deep coverage of cellular proteomes. To achieve near-comprehensive identification and quantification, the combination of a first HPLC-based peptide fractionation orthogonal to the online LC-MS/MS step has proven to be particularly powerful. This first dimension is typically performed with mL/min-flow and relatively large column inner diameters, which allows efficient pre-fractionation but typically requires peptide amounts in the mg range...
January 26, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28114147/physical-activity-and-sleep-patterns-in-hemodialysis-patients-in-a-suburban-environment
#12
Schantel Williams, Maggie Han, Xiaoling Ye, Hanjie Zhang, Anna Meyring-Wösten, Marcee Bonner, Candace Young, Stephan Thijssen, Daniel Marsh, Peter Kotanko
BACKGROUND/AIMS: Hemodialysis (HD) patients are less active than their healthy counterparts. They are often plagued with sleep disorders that affect the quality of their sleep. Our aim was to objectively quantify activity and sleep quality among HD patients in a suburban HD population. METHODS: Activity and sleep parameters were measured using a commercially available activity tracker in 29 HD patients from Baton Rouge, LA, USA. Patients in the feedback group received their activity and sleep data at each dialysis treatment...
January 24, 2017: Blood Purification
https://www.readbyqxmd.com/read/28112946/temporal-dynamics-of-in-field-bioreactor-populations-reflect-the-groundwater-system-and-respond-predictably-to-perturbation
#13
Andrew J King, Sarah P Preheim, Kathryn L Bailey, Michael S Robeson, Taniya Roy Chowdhury, Bryan R Crable, Richard A Hurt, Tonia L Mehlhorn, Kenneth A Lowe, Tommy J Phelps, Anthony V Palumbo, Craig C Brandt, Steven D Brown, Mircea Podar, Ping Zhang, William Andrew Lancaster, Farris L Poole, David B Watson, Matthew W W Fields, John-Marc Chandonia, Eric J Alm, Jizhong Zhou, Michael W W Adams, Terry C Hazen, Adam P Arkin, Dwayne A Elias
Temporal variability complicates testing the influences of environmental variability on microbial community structure and thus function. An in-field bioreactor system was developed to assess oxic versus anoxic manipulations on in-situ groundwater communities. Each sample was sequenced (16S SSU rRNA genes, average 10,000 reads) and biogeochemical parameters monitored by quantifying 53 metals, 12 organic acids, 14 anions and 3 sugars. Changes in dissolved oxygen (DO), pH, and other variables were similar across bioreactors...
January 23, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28109528/specific-enrichment-of-a-targeted-nitrotyrosine-containing-peptide-from-complex-matrices-and-relative-quantification-for-liquid-chromatography-mass-spectrometry-analysis
#14
Yun Yang
Protein tyrosine nitration is considered an important non-enzymatic post-translational modification. In the tyrosine nitration process, 3-nitrotyrosine is formed and recognized as a biomarker of nitrosative/nitrative stress implicated in inflammatory responses and age-related disorders. In view of the complexity of biological samples and the ultra-low abundance of protein-incorporated nitrotyrosine, selective enrichment of nitrotyrosine-containing peptides prior to chromatographic separation is crucial. Herein, I report a simple yet highly specific and efficient enrichment method for nitrotyrosine-containing peptides...
February 17, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28109439/deep-profiling-of-proteome-and-phosphoproteome-by-isobaric-labeling-extensive-liquid-chromatography-and-mass-spectrometry
#15
B Bai, H Tan, V R Pagala, A A High, V P Ichhaporia, L Hendershot, J Peng
Mass spectrometry-based proteomics has experienced an unprecedented advance in comprehensive analysis of proteins and posttranslational modifications, with particular technical progress in liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and isobaric labeling multiplexing capacity. Here, we introduce a deep proteomics profiling protocol that combines 10-plex tandem mass tag (TMT) labeling with an optimized LC-MS/MS platform to quantitate whole proteome and phosphoproteome. The major steps include protein extraction and digestion, TMT labeling, two-dimensional liquid chromatography, TiO2-mediated phosphopeptide enrichment, high-resolution mass spectrometry, and computational data processing...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28103405/altered-walking-strategy-and-increased-unsteadiness-in-participants-with-impaired-glucose-tolerance-and-type-2-diabetes-relates-to-small-fibre-neuropathy-but-not-vitamin-d-deficiency
#16
M M Almurdhi, S J Brown, F L Bowling, A J M Boulton, M Jeziorska, R A Malik, N D Reeves
AIMS: To investigate alterations in walking strategy and dynamic sway (unsteadiness) in people with impaired glucose tolerance and people with Type 2 diabetes in relation to severity of neuropathy and vitamin D levels. METHODS: A total of 20 people with Type 2 diabetes, 20 people with impaired glucose tolerance and 20 people without either Type 2 diabetes or impaired glucose tolerance (control group) underwent gait analysis using a motion analysis system and force platforms, and detailed assessment of neuropathy and serum 25 hydroxy-vitamin D levels...
January 19, 2017: Diabetic Medicine: a Journal of the British Diabetic Association
https://www.readbyqxmd.com/read/28093573/impact-of-lifestyle-intervention-for-obese-women-during-pregnancy-on-maternal-metabolic-and-inflammatory-markers
#17
K M Renault, E M Carlsen, S Hædersdal, L Nilas, N J Secher, J Eugen-Olsen, D Cortes, S F Olsen, T I Halldorsson, K Nørgaard
BACKGROUND: Offspring of obese mothers have increased risk of developing obesity and related short and long-term disease. The cause is multifactorial and may partly be explained by the unfavorable intrauterine environment. Intervention during pregnancy leading to a healthier lifestyle among obese may alter this. OBJECTIVE: To assess the effect of lifestyle intervention on markers of maternal metabolism and inflammation in 'the TOP (Treatment of Obese Pregnant Women)-study', a randomized controlled trial...
January 17, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28087535/efficient-low-ph-iron-removal-by-a-microbial-iron-oxide-mound-ecosystem-at-scalp-level-run
#18
Christen L Grettenberger, Alexandra R Pearce, Kyle J Bibby, Daniel S Jones, William D Burgos, Jennifer L Macalady
: Acid mine drainage (AMD) is a major environmental problem affecting tens of thousands of km of waterways worldwide. Passive bioremediation of AMD relies on microbial communities to oxidize and remove iron from the system; however, iron-oxidation rates in AMD environments are highly variable across sites. At Scalp Level Run (Cambria County PA), first-order iron-oxidation rates are ten times faster than at other coal associated iron mounds in the Appalachians. We examined the bacterial community at Scalp Level Run to determine whether a unique community is responsible for the rapid iron oxidation rate...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28073970/estimated-generic-prices-for-novel-treatments-for-drug-resistant-tuberculosis
#19
Dzintars Gotham, Joseph Fortunak, Anton Pozniak, Saye Khoo, Graham Cooke, Frederick E Nytko, Andrew Hill
BACKGROUND: The estimated worldwide annual incidence of MDR-TB is 480 000, representing 5% of TB incidence, but 20% of mortality. Multiple drugs have recently been developed or repurposed for the treatment of MDR-TB. Currently, treatment for MDR-TB costs thousands of dollars per course. OBJECTIVES: To estimate generic prices for novel TB drugs that would be achievable given large-scale competitive manufacture. METHODS: Prices for linezolid, moxifloxacin and clofazimine were estimated based on per-kilogram prices of the active pharmaceutical ingredient (API)...
January 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28068968/lifestyle-behaviours-associated-with-5-year-weight-gain-in-a-prospective-cohort-of-australian-adults-aged-26-36-years-at-baseline
#20
Kylie J Smith, Seana L Gall, Sarah A McNaughton, Verity J Cleland, Petr Otahal, Terence Dwyer, Alison J Venn
BACKGROUND: Whether not meeting common guidelines for lifestyle behaviours is associated with weight gain is uncertain. This study examined whether 5-year weight gain was predicted by not meeting guidelines for: breakfast consumption (eating between 6 and 9 am), takeaway food consumption (<2 times/week), television viewing (<2 h/day) and daily steps (≥10,000 steps/day). METHODS: One thousand one hundred and fifty-five Australian participants (43% men, 26-36 years) completed questionnaires and wore a pedometer at baseline (2004-06) and follow-up (2009-11)...
January 10, 2017: BMC Public Health
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