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Kodela Vani, Seshi R Sompuram, Anika K Schaedle, Anuradha Balasubramanian, Steven A Bogen
An important limitation in the field of immunohistochemistry (IHC) is the inability to correlate stain intensity with specific analyte concentrations. Clinical immunohistochemical tests are not described in terms of analytic response curves, namely, the analyte concentrations in a tissue sample at which an immunohistochemical stain (1) is first visible, (2) increases in proportion to the analyte concentration, and (3) ultimately approaches a maximum color intensity. Using a new immunostaining tool ( IHControls), we measured the analytic response curves of the major clinical immunohistochemical tests for human epidermal growth factor receptor type II (HER-2), estrogen receptor (ER), and progesterone receptor (PR)...
May 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
Elena Kupcová, Katarína Reiffová
In this work, a fast and effective dispersive liquid-liquid microextraction was developed for the isolation and pre-concentration of free 17β-estradiol, the main human estrogen, from real human urine samples. To optimize the extraction technique, few important parameters such as type and volume of extraction and dispersive solvents, centrifugation conditions, effect of salt addition, and extraction time were studied. Optimal conditions were obtained when injecting 600 μL mixture of tetrachloromethane as extraction solvent and ethanol as dispersive solvent (1:5; v/v) into 2 mL of urine containing 8% NaCl and following centrifugation at 10 000 rpm, thus reaching enrichment factor 28 and extraction recovery 98% for estradiol...
April 24, 2017: Journal of Separation Science
Brett J Feldman, Cristina G Calogero, Kareem S Elsayed, Osman Z Abbasi, Joshua Enyart, Timothy J Friel, Yasir H Abunamous, Stephen W Dusza, Marna Rayl Greenberg
INTRODUCTION: According to the National Alliance to End Homelessness, the national rate of homelessness has been cited as 17.7 homeless people/10,000 people in the general population, and 24.8 homeless veterans/10,000 veterans in the general population. However, it is unknown what the prevalence of homelessness is in the emergency department (ED) setting. We set out to determine the prevalence of homelessness or at risk for homelessness in the ED setting. METHODS: Using a five-question screening tool derived from the U...
April 2017: Western Journal of Emergency Medicine
Fabrice Gritti, Michael Fogwill
The potential advantage of turbulent supercritical fluid chromatography (TSFC) in open tubular columns (OTC) was evaluated on both theoretical and practical viewpoints. First, the dispersion model derived by Golay in 1958 and recently extended from laminar to turbulent flow regime is used for the predictions of the speed-resolution performance in TSFC. The average dispersion coefficient of matter in the turbulent flow regime was taken from the available experimental data over a range of Reynolds number from 2000 to 6000...
April 19, 2017: Journal of Chromatography. A
Steven A Harvey, Sandra Incardona, Nina Martin, Cristina Lussiana, Elizabeth Streat, Stephanie Dolan, Nora Champouillon, Daniel J Kyabayinze, Robert Mugerwa, Grace Nakanwagi, Nancy Njoki, Ratsimandisa Rova, Jane Cunningham
BACKGROUND: Use of antigen-detecting malaria rapid diagnostic tests (RDTs) has increased exponentially over the last decade. WHO's Global Malaria Programme, FIND, and other collaborators have established a quality assurance scheme to guide product selection, lot verification, transport, storage, and training procedures. Recent concerns over the quality of buffer packaging and test accessories suggest a need to include these items in product assessments. This paper describes quality problems with buffer and accessories encountered in a project promoting private sector RDT use in five African countries and suggests steps to avoid or more rapidly identify and resolve such problems...
April 20, 2017: Malaria Journal
David R Bell, Karin A Pfeiffer, Lisa A Cadmus-Bertram, Stephanie M Trigsted, Adam Kelly, Eric G Post, Joseph M Hart, Dane B Cook, Warren R Dunn, Christopher Kuenze
BACKGROUND: Returning to a healthy level of physical activity is among the most commonly discussed clinical goals for patients recovering from anterior cruciate ligament reconstruction (ACLR). However, physical activity has not been objectively measured in this population. PURPOSE: To investigate differences in the mean time (min/d) spent in moderate-to-vigorous physical activity (MVPA) as well as the daily step count (steps/d) between patients who underwent ACLR and matched controls...
April 1, 2017: American Journal of Sports Medicine
Emerson Sebastião, Yvonne C Learmonth, Robert W Motl
Persons with multiple sclerosis (MS) often report being afraid of falling, and this may have effects on physical activity (PA) engagement. This study investigated PA levels in persons with MS as a function of fall risk categories. Forty-seven persons with MS participated in the study and were categorized into either increased fall risk (IFR; n = 21; 55.5 ± 9.0 years) or normal fall risk (NFR; n = 26; 51.2 ± 12.9 years) groups based on scores from the Activities-Balance Confidence scale. PA was measured by accelerometer and expressed as average steps per day, and time spent in sedentary behavior, light PA, and moderate to vigorous physical activity over the course of 7 consecutive days...
May 2017: American Journal of Physical Medicine & Rehabilitation
R L Wang, C Y Yuan, Y X Pan, F C Tian, Z H Wang, X Y Wang
Objective: To investigate surface properties of novel flowable composites after polishing and simulated brushing wear, compared to their pasty counterpart. Methods: Composites employed in this study were: three flowable composites (A1: Clearfil Majesty ES Flow; B1: Beautifil Flow Plus F00; C1: Filtek Bulk Fill) and three paste composites (A2: Clearfil Majesty; B2: Beautifil; C2: Filtek Z350. Eleven disk-shaped specimens were made for each material. The specimens were cured, then subjected to sandpaper finishing for 20 s, one-step polishing for 30 s, finally subjected to simulated brushing for 10 000 cycles...
April 9, 2017: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
Bárbara C Leal, Guilherme L P Aydos, Paulo A Netz, Jairton Dupont
The isomerization of estragole to trans-anethole is an important reaction and is industrially performed using an excess of NaOH or KOH in ethanol at high temperatures with very low selectivity. Simple Ru-based transition-metal complexes, under homogeneous, ionic liquid (IL)-supported (biphasic) and "solventless" conditions, can be used for this reaction. The selectivity of this reaction is more sensitive to the solvent/support used than the ligands associated with the metal catalyst. Thus, under the optimized reaction conditions, 100% conversion can be achieved in the estragole isomerization, using as little as 4 × 10(-3) mol % (40 ppm) of [RuHCl(CO)(PPh3)3] in toluene, reflecting a total turnover number (TON) of 25 000 and turnover frequencies (TOFs) of up to 500 min(-1) at 80 °C...
March 31, 2017: ACS Omega
Mahshid Mohammadi-Bassir, Mahdieh Jamshidian, Mohammad Bagher Rezvani, Mansoure Babasafari
STATEMENT OF PROBLEM: Limited information is available for the best polishing systems and methods to obtain minimally abrasive monolithic zirconia surfaces after contouring and occlusal adjustment. PURPOSE: The purpose of this in vitro study was to evaluate the effect of grinding and polishing procedures on the flexural strength, quality and quantity of surface roughness, topography, and phase transformation of a zirconia-based ceramic system. MATERIAL AND METHODS: Fifty bar-shaped yttrium-stabilized zirconium oxide specimens (20×4×2 mm) were cut from presintered zirconia blanks...
April 3, 2017: Journal of Prosthetic Dentistry
Chee Keong Kwok, Yuichiro Ueda, Asifiqbal Kadari, Katharina Günther, Antoine Heron, Aletta C Schnitzler, Martha Rook, Frank Edenhofer
The production of human induced pluripotent stem cells (hiPSCs) in quantities that are relevant for cell-based therapies and cell-loaded implants through standard adherent culture is hardly achievable and lacks process scalability. A promising approach to overcoming these hurdles is the culture of hiPSCs in suspension. In this study, stirred suspension culture vessels were investigated for their suitability in the expansion of two hiPSC lines inoculated as a single cell suspension, with a free scalability between volumes of 50 and 2 400 mL...
April 6, 2017: Journal of Tissue Engineering and Regenerative Medicine
Alexander M Sherwood, Rachel Saylor Crowley, Kelly F Paton, Andrew Biggerstaff, Benjamin Neuenswander, Victor W Day, Bronwyn M Kivell, Thomas E Prisinzano
Previous structure-activity studies on the neoclerodane diterpenoid salvinorin A have demonstrated the importance of the acetoxy functionality on the A-ring in its activity as a κ-opioid receptor agonist. Few studies have focused on understanding the role of conformation in these interactions. Herein we describe the synthesis and evaluation of both flexible and conformationally restricted compounds derived from salvinorin A. One such compound, spirobutyrolactone 14, was synthesized in a single step from salvinorin B and had similar potency and selectivity to salvinorin A (EC50 = 0...
April 19, 2017: Journal of Medicinal Chemistry
Zhi Zheng, Weiping Wang, Xin Huang, Quan Lv, Wenling Fan, Wenkui Yu, Lei Li, Zhibing Zhang
Polysulfone (PSF) was grafted chemically with polyethylene glycol (PEG) of different molecular weights (400, 2000, 10 000, and 20 000) and heparin to prepare PSF-PEG-Hep membranes (labeled as PSF-PEG400-Hep, PSF-PEG2000-Hep, PSF-PEG10 000-Hep, and PSF-PEG20 000-Hep). These membranes were synthesized via the following steps: (i) PSF chloromethylation; (ii) PEGylation; and (iii) heparin immobilization process. Water contact angle, critical water permeation pressure, ATR-FTIR, (1) H NMR, UV-visible spectrophotometry, and X-ray photoelectron spectroscopy were conducted to prove grafting success and to fix the optimal reaction parameters during chemical modification processes...
November 2016: Artificial Organs
Jorge De-Las-Heras-Romero, Ana M Lledo-Alvarez, Alejandro Lizaur-Utrilla, Fernando A Lopez-Prats
BACKGROUND: Intra-articular tibial plafond fractures are a challenge for the surgeon with generally poor functional outcomes. The purpose was to assess the impact of this injury on patient quality of life (QOL), as well as analyzed the predictor factors. METHODS: Retrospective study of 47 patients with mean age of 44.4 (range 18-79) years. Patients were also invited for new clinical and radiological assessments. QOL was evaluated by the Short Form-36 questionnaire and compared to the standard in the age-matched general population...
March 22, 2017: Injury
Renata A Rodrigues de Oliveira, Paulo R Dos Santos Amorim, Rômulo J Mota, Debora D Ferreira Tavares, Fernanda Rocha de Faria, Osvaldo Costa Moreira, Luciana Moreira Lima, Raquel Rodrigues Britto, João C Bouzas Marins
BACKGROUND: Regular physical activity is suggested as an important non pharmacological mechanism to prevent cardiovascular diseases. Thus, the objective the presente study was to verify the association of the number of daily steps with cardiovascular risk factors in basic education teachers and analyze the suitability of the cutoff of 10,000 steps/day as a predictor for the main risk factors in this sample. METHODS: 200 teachers (43.2 + 10.2 years) of basic education were assessed in Viçosa-MG, Brazil...
March 30, 2017: Journal of Sports Medicine and Physical Fitness
Marc A Adams, Jane C Hurley, Michael Todd, Nishat Bhuiyan, Catherine L Jarrett, Wesley J Tucker, Kevin E Hollingshead, Siddhartha S Angadi
BACKGROUND: Emerging interventions that rely on and harness variability in behavior to adapt to individual performance over time may outperform interventions that prescribe static goals (e.g., 10,000 steps/day). The purpose of this factorial trial was to compare adaptive vs. static goal setting and immediate vs. delayed, non-contingent financial rewards for increasing free-living physical activity (PA). METHODS: A 4-month 2 × 2 factorial randomized controlled trial tested main effects for goal setting (adaptive vs...
March 29, 2017: BMC Public Health
Andreas Ranft, Corinna Seidel, Christiane Hoffmann, Michael Paulussen, Ann-Christin Warby, Henk van den Berg, Ruth Ladenstein, Claudia Rossig, Uta Dirksen, Dieter Rosenbaum, Herbert Juergens
Purpose Significantly improved survival rates in patients with Ewing sarcoma have raised interest in accessing the quality of long-term survivorship. In this study, subjective and objective measurement tools, preclassified as physical or mental scores, were used to assess clinicofunctional outcome and physical activity after intensive bone tumor treatment. Methods Long-term outcome of 618 survivors from consecutive Ewing sarcoma trials was assessed by the Toronto Extremity Salvage Score, Short-Form Health Survey (SF-36), Brief Symptom Inventory (BSI), and Rosenberg Self-Esteem Scale questionnaires and by the accelerometric StepWatch 3 Activity Monitor...
March 29, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
Rajendra P Panmand, Purnima Patil, Yogesh Sethi, Sunil R Kadam, Milind V Kulkarni, Suresh W Gosavi, N R Munirathnam, Bharat B Kale
Herein, we demonstrated a green approach for the synthesis of high surface area (850 m(2) g(-1)) mesoporous perforated graphene (PG) from Bougainvillea flower for the first time using a template free single-step method. The existence of PG was confirmed by XRD, Raman spectroscopy, FESEM, and FETEM. Surprisingly, FETEM clearly showed 5-10 nm perforation on the graphene sheets. More significantly, these mesoporous perforated graphene sheets can be produced in large scale using the present green approach. Considering high surface area and unique perforated graphene architecture, these PGs were studied for supercapacitor applications in detail without any chemical or physical activation...
March 29, 2017: Nanoscale
Marta Futema, Jackie A Cooper, Marietta Charakida, Christopher Boustred, Naveed Sattar, John Deanfield, Debbie A Lawlor, Nicholas J Timpson, Steve E Humphries, Aroon D Hingorani
BACKGROUND AND AIMS: Familial hypercholesterolaemia (FH) is an autosomal-dominant disease with frequency of 1/500 to 1/250 that leads to premature coronary heart disease. New approaches to identify FH mutation-carriers early are needed to prevent premature cardiac deaths. In a cross-sectional study of the Avon Longitudinal Study of Parents and Children (ALSPAC), we evaluated the biochemical thresholds for FH screening in childhood, and modelled a two-stage biochemical and sequencing screening strategy for FH detection...
May 2017: Atherosclerosis
Karolina Haernvall, Sabine Zitzenbacher, Katrin Wallig, Motonori Yamamoto, Michael Bernhard Schick, Doris Ribitsch, Georg M Guebitz
Water-soluble polyesters are used in a range of applications today and enter wastewater treatment plants after product utilization. However, little is known about extracellular enzymes and aquatic microorganisms involved in polyester biodegradation and mineralization. In this study, structurally different ionic phthalic acid based polyesters (the number-average molecular weights (Mn) 1770 to 10 000 g/mol and semi crystalline with crystallinity below 1%) were synthesized in various combinations. Typical wastewater microorganisms like Pseudomonas sp...
April 7, 2017: Environmental Science & Technology
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