keyword
MENU ▼
Read by QxMD icon Read
search

10,000 steps

keyword
https://www.readbyqxmd.com/read/28323221/laparoscopic-promontohysteropexy-during-pregnancy
#1
L Pirtea, O Balint, C Secosan, R Ilina, D Grigoras
STUDY OBJECTIVE: to present a case of uterine prolapse in a 10 weeks pregnant patient that was successfully treated by laparoscopic promontohysteropexy. To our knowledge, this is the first case of laparoscopic promontopexy during pregnancy to ever be reported in literature. DESIGN: step by step explanation of the surgery using video SETTING: Uterine prolapse is a rare condition in young patients and extremely are in pregnant women. The incidence of uterine prolapse during pregnancy reported in literature is 1 per 10...
March 17, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28286354/how-did-the-domestication-of-fertile-crescent-grain-crops-increase-their-yields
#2
Catherine Preece, Alexandra Livarda, Pascal-Antoine Christin, Michael Wallace, Gemma Martin, Michael Charles, Glynis Jones, Mark Rees, Colin P Osborne
The origins of agriculture, 10 000 years ago, led to profound changes in the biology of plants exploited as grain crops, through the process of domestication. This special case of evolution under cultivation led to domesticated cereals and pulses requiring humans for their dispersal, but the accompanying mechanisms causing higher productivity in these plants remain unknown. The classical view of crop domestication is narrow, focusing on reproductive and seed traits including the dispersal, dormancy and size of seeds, without considering whole-plant characteristics...
February 2017: Functional Ecology
https://www.readbyqxmd.com/read/28279451/data-driven-models-to-predict-the-elimination-of-sleeping-sickness-in-former-equateur-province-of-drc
#3
K S Rock, A Pandey, M L Ndeffo-Mbah, K E Atkins, C Lumbala, A Galvani, M J Keeling
Approaching disease elimination, it is crucial to be able to assess progress towards key objectives using quantitative tools. For Gambian human African trypanosomiasis (HAT), the ultimate goal is to stop transmission by 2030, while intermediary targets include elimination as a public health problem - defined as <1 new case per 10,000 inhabitants in 90% of foci, and <2000 reported cases by 2020. Using two independent mathematical models, this study assessed the achievability of these goals in the former Equateur province of the Democratic Republic of Congo, which historically had endemic levels of disease...
March 2017: Epidemics
https://www.readbyqxmd.com/read/28257108/a-new-strategy-to-reduce-influenza-escape-detecting-therapeutic-targets-constituted-of-invariance-groups
#4
Julie Lao, Anne Vanet
The pathogenicity of the different flu species is a real public health problem worldwide. To combat this scourge, we established a method to detect drug targets, reducing the possibility of escape. Besides being able to attach a drug candidate, these targets should have the main characteristic of being part of an essential viral function. The invariance groups that are sets of residues bearing an essential function can be detected genetically. They consist of invariant and synthetic lethal residues (interdependent residues not varying or slightly varying when together)...
March 2, 2017: Viruses
https://www.readbyqxmd.com/read/28255713/quantitative-phosphoproteomic-analysis-of-t-cell-receptor-signaling
#5
Nagib Ahsan, Arthur R Salomon
TCR signaling critically depends on protein phosphorylation across many proteins. Localization of each phosphorylation event relative to the T-cell receptor (TCR) and canonical T-cell signaling proteins will provide clues about the structure of TCR signaling networks. Quantitative phosphoproteomic analysis by mass spectrometry provides a wide-scale view of cellular phosphorylation networks. However, analysis of phosphorylation by mass spectrometry is still challenging due to the relative low abundance of phosphorylated proteins relative to all proteins and the extraordinary diversity of phosphorylation sites across the proteome...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28228337/minimizing-the-mandibular-advancement-in-an-oral-appliance-for-the-treatment-of-obstructive-sleep-apnea
#6
Eduardo Anitua, Joaquín Durán-Cantolla, Gabriela Zamora Almeida, Mohammad Hamdan Alkhraisat
OBJECTIVE: In the treatment of obstructive sleep apnea (OSA) with an oral appliance (OA), there is no gold standard method to fine-tune the mandibular advancement. This study aimed to analyze the effect of gradual increment of mandibular advancement on the evolution of the apnea-hypopnea index (AHI). METHODS: OSA patients were recruited from a sleep unit. All treatments started with an oral appliance without mandibular advancement. After two weeks, the AHI was assessed with respiratory polygraphy...
January 29, 2017: Sleep Medicine
https://www.readbyqxmd.com/read/28196113/chasm-in-primary-care-provision-in-a-universal-health-system-findings-from-a-nationally-representative-survey-of-health-facilities-in-malaysia
#7
Huy Ming Lim, Sheamini Sivasampu, Ee Ming Khoo, Kamaliah Mohamad Noh
BACKGROUND: Malaysia has achieved universal health coverage since 1980s through the expansion of direct public provision, particularly in rural areas. However, no systematic examination of the rural-urban distribution of primary care services and resources has been conducted to date for policy impact evaluation. METHODS: We conducted a national cross-sectional survey of 316 public and 597 private primary care clinics, selected through proportionate stratified random sampling, from June 2011 through February 2012...
2017: PloS One
https://www.readbyqxmd.com/read/28188524/hydrophilic-strong-anion-exchange-hsax-chromatography-enables-deep-fractionation-of-tissue-proteomes
#8
Benjamin Ruprecht, Dongxue Wang, Riccardo Zenezini Chiozzi, Li-Hua Li, Hannes Hahne, Bernhard Kuster
The bottom-up proteomic analysis of cell line and tissue samples to a depth > 10,000 proteins still represents a considerable challenge because of the sheer number of peptides generated by proteolytic digestions and the high dynamic range of protein expression. As a result, comprehensive protein coverage requires multidimensional peptide separation. Recently, off-line hydrophilic strong cation exchange (hSAX) chromatography has proven its merits for high resolution separation of peptides due to its high degree of orthogonality to reversed-phase liquid chromatography...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28188522/optimized-enrichment-of-phosphoproteomes-by-fe-imac-column-chromatography
#9
Benjamin Ruprecht, Heiner Koch, Petra Domasinska, Martin Frejno, Bernhard Kuster, Simone Lemeer
Phosphorylation is among the most important post-translational modifications of proteins and has numerous regulatory functions across all domains of life. However, phosphorylation is often substoichiometric, requiring selective and sensitive methods to enrich phosphorylated peptides from complex cellular digests. Various methods have been devised for this purpose and we have recently described a Fe-IMAC HPLC column chromatography setup which is capable of comprehensive, reproducible, and selective enrichment of phosphopeptides out of complex peptide mixtures...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28178955/the-management-of-the-faeces-passed-by-under-five-children-an-exploratory-cross-sectional-research-in-an-urban-community-in-southwest-nigeria
#10
Olufemi Oludare Aluko, Olusegun Temitope Afolabi, Emmanuel Abiodun Olaoye, Adeyinka Daniel Adebayo, Seun Oladele Oyetola, Oluwaseun Olamide Abegunde
BACKGROUND: Safe management of faeces (SMoF) and environmental contamination by faecal pathogens have been extensively researched although the SMoF in under-five children has been perennially neglected perhaps due to the misconception that it is harmless. This research, therefore, studied the situation, to determine the magnitude and dimensions of the problem aimed at making policy level stakeholders aware of child faeces management systems and so, inform evidence-based implementation of child and health-related programmes in Nigeria...
February 8, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28177727/associations-between-physical-activity-screen-time-and-fitness-among-6-to-10-year-old-children-living-in-edmonton-canada
#11
Morgan Potter, John C Spence, Normand G Boulé, Jodie A Stearns, Valerie Carson
The objectives of this study were to describe fitness levels; examine associations between physical activity (PA), screen time (ST), and fitness; and examine sex-moderating effects in a sample of children. Participants were 649 children (age, 7.8 ± 0.6 years; 52.4% female) from Edmonton, Canada. Hours/week of PA and ST were parent-reported. PA was also objectively measured with pedometers and expressed in increments of 1000 steps/day. Fitness components (i.e., vertical jump, sit-and-reach, waist circumference, grip strength, predicted maximal oxygen consumption, push-ups, partial curl-ups, overall fitness) were measured according to the Canadian Physical Activity, Fitness, and Lifestyle Approach protocols and expressed as z scores or low/high fitness...
January 3, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28177357/technical-note-avoiding-the-direct-inversion-of-the-numerator-relationship-matrix-for-genotyped-animals-in-single-step-genomic-best-linear-unbiased-prediction-solved-with-the-preconditioned-conjugate-gradient
#12
Y Masuda, I Misztal, A Legarra, S Tsuruta, D A L Lourenco, B O Fragomeni, I Aguilar
This paper evaluates an efficient implementation to multiply the inverse of a numerator relationship matrix for genotyped animals () by a vector (). The computation is required for solving mixed model equations in single-step genomic BLUP (ssGBLUP) with the preconditioned conjugate gradient (PCG). The inverse can be decomposed into sparse matrices that are blocks of the sparse inverse of a numerator relationship matrix () including genotyped animals and their ancestors. The elements of were rapidly calculated with the Henderson's rule and stored as sparse matrices in memory...
January 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28138134/time-spent-in-sedentary-posture-is-associated-with-waist-circumference-and-cardiovascular-risk
#13
W W Tigbe, M H Granat, N Sattar, M E J Lean
BACKGROUND: The relationship between metabolic risk and time spent sitting, standing and stepping has not been well established. The present study aimed to determine associations of objectively measured time spent siting, standing and stepping, with coronary heart disease (CHD) risk. METHODS: A cross-sectional study of healthy non-smoking Glasgow postal workers, n=111 (55 office-workers, 5 women, and 56 walking/delivery-workers, 10 women), who wore activPAL physical activity monitors for seven days...
January 31, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28128213/high-throughput-automated-microfluidic-sample-preparation-for-accurate-microbial-genomics
#14
Soohong Kim, Joachim De Jonghe, Anthony B Kulesa, David Feldman, Tommi Vatanen, Roby P Bhattacharyya, Brittany Berdy, James Gomez, Jill Nolan, Slava Epstein, Paul C Blainey
Low-cost shotgun DNA sequencing is transforming the microbial sciences. Sequencing instruments are so effective that sample preparation is now the key limiting factor. Here, we introduce a microfluidic sample preparation platform that integrates the key steps in cells to sequence library sample preparation for up to 96 samples and reduces DNA input requirements 100-fold while maintaining or improving data quality. The general-purpose microarchitecture we demonstrate supports workflows with arbitrary numbers of reaction and clean-up or capture steps...
January 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/28127346/trypanosoma-cruzi-evaluation-of-pcr-as-a-laboratory-tool-to-follow-up-the-evolution-of-parasite-load
#15
Fabiana Nabarro Ferraz, Denise Lessa Aleixo, Ana Paula Gruendling, Mônica Lúcia Gomes, Max Jean de Ornelas Toledo, Silvana Marques DE Araújo
BACKGROUND: The study evaluated qualitative PCR, primers 121-122 as a tool to follow up evolution parasite load of Trypanosoma cruzi. METHODS: The study was conducted at the State University of Maringa, in 2015. Step 1, dilutions 1/10 were performed from T. cruzi-Y strain to obtain preparations of 50,000-0.05 parasites/mL from which DNA were extracted, quantified, and amplified. Step 2, the extracted DNA in the dilutions 5-0.05 parasites/mL was re-diluted 1/10, 1/100, 1/1000, quantified, and amplified...
July 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28126900/loss-less-nano-fractionator-for-high-sensitivity-high-coverage-proteomics
#16
Nils A Kulak, Philipp E Geyer, Matthias Mann
Recent advances in mass spectrometry (MS)-based proteomics now allow very deep coverage of cellular proteomes. To achieve near-comprehensive identification and quantification, the combination of a first HPLC-based peptide fractionation orthogonal to the online LC-MS/MS step has proven to be particularly powerful. This first dimension is typically performed with mL/min-flow and relatively large column inner diameters, which allows efficient pre-fractionation but typically requires peptide amounts in the mg range...
January 26, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28114147/physical-activity-and-sleep-patterns-in-hemodialysis-patients-in-a-suburban-environment
#17
RANDOMIZED CONTROLLED TRIAL
Schantel Williams, Maggie Han, Xiaoling Ye, Hanjie Zhang, Anna Meyring-Wösten, Marcee Bonner, Candace Young, Stephan Thijssen, Daniel Marsh, Peter Kotanko
BACKGROUND/AIMS: Hemodialysis (HD) patients are less active than their healthy counterparts. They are often plagued with sleep disorders that affect the quality of their sleep. Our aim was to objectively quantify activity and sleep quality among HD patients in a suburban HD population. METHODS: Activity and sleep parameters were measured using a commercially available activity tracker in 29 HD patients from Baton Rouge, LA, USA. Patients in the feedback group received their activity and sleep data at each dialysis treatment...
2017: Blood Purification
https://www.readbyqxmd.com/read/28112946/temporal-dynamics-of-in-field-bioreactor-populations-reflect-the-groundwater-system-and-respond-predictably-to-perturbation
#18
Andrew J King, Sarah P Preheim, Kathryn L Bailey, Michael S Robeson, Taniya Roy Chowdhury, Bryan R Crable, Richard A Hurt, Tonia Mehlhorn, Kenneth A Lowe, Tommy J Phelps, Anthony V Palumbo, Craig C Brandt, Steven D Brown, Mircea Podar, Ping Zhang, W Andrew Lancaster, Farris Poole, David B Watson, Matthew W Fields, John-Marc Chandonia, Eric J Alm, Jizhong Zhou, Michael W W Adams, Terry C Hazen, Adam P Arkin, Dwayne A Elias
Temporal variability complicates testing the influences of environmental variability on microbial community structure and thus function. An in-field bioreactor system was developed to assess oxic versus anoxic manipulations on in situ groundwater communities. Each sample was sequenced (16S SSU rRNA genes, average 10,000 reads), and biogeochemical parameters are monitored by quantifying 53 metals, 12 organic acids, 14 anions, and 3 sugars. Changes in dissolved oxygen (DO), pH, and other variables were similar across bioreactors...
February 10, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28109528/specific-enrichment-of-a-targeted-nitrotyrosine-containing-peptide-from-complex-matrices-and-relative-quantification-for-liquid-chromatography-mass-spectrometry-analysis
#19
Yun Yang
Protein tyrosine nitration is considered an important non-enzymatic post-translational modification. In the tyrosine nitration process, 3-nitrotyrosine is formed and recognized as a biomarker of nitrosative/nitrative stress implicated in inflammatory responses and age-related disorders. In view of the complexity of biological samples and the ultra-low abundance of protein-incorporated nitrotyrosine, selective enrichment of nitrotyrosine-containing peptides prior to chromatographic separation is crucial. Herein, I report a simple yet highly specific and efficient enrichment method for nitrotyrosine-containing peptides...
February 17, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28109439/deep-profiling-of-proteome-and-phosphoproteome-by-isobaric-labeling-extensive-liquid-chromatography-and-mass-spectrometry
#20
B Bai, H Tan, V R Pagala, A A High, V P Ichhaporia, L Hendershot, J Peng
Mass spectrometry-based proteomics has experienced an unprecedented advance in comprehensive analysis of proteins and posttranslational modifications, with particular technical progress in liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and isobaric labeling multiplexing capacity. Here, we introduce a deep proteomics profiling protocol that combines 10-plex tandem mass tag (TMT) labeling with an optimized LC-MS/MS platform to quantitate whole proteome and phosphoproteome. The major steps include protein extraction and digestion, TMT labeling, two-dimensional liquid chromatography, TiO2-mediated phosphopeptide enrichment, high-resolution mass spectrometry, and computational data processing...
2017: Methods in Enzymology
keyword
keyword
97048
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"