keyword
MENU ▼
Read by QxMD icon Read
search

In vitro diagnostic technology

keyword
https://www.readbyqxmd.com/read/28074431/detection-and-specific-elimination-of-egfr-ovarian-cancer-cells-using-a-near-infrared-photoimmunotheranostic-approach
#1
Dirk Bauerschlag, Ivo Meinhold-Heerlein, Nicolai Maass, Andreas Bleilevens, Karen Bräutigam, Wa'el Al Rawashdeh, Stefano Di Fiore, Anke Maria Haugg, Felix Gremse, Julia Steitz, Rainer Fischer, Elmar Stickeler, Stefan Barth, Ahmad Fawzi Hussain
PURPOSE: Targeted theranostics is an alternative strategy in cancer management that aims to improve cancer detection and treatment simultaneously. This approach combines potent therapeutic and diagnostic agents with the specificity of different cell receptor ligands in one product. The success of antibody drug conjugates (ADCs) in clinical practice has encouraged the development of antibody theranostics conjugates (ATCs). However, the generation of homogeneous and pharmaceutically-acceptable ATCs remains a major challenge...
January 10, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28068664/fusion-peptide-improves-stability-and-bioactivity-of-single-chain-antibody-against-rabies-virus
#2
Hualong Xi, Kaixin Zhang, Yanchun Yin, Tiejun Gu, Qing Sun, Linqing Shi, Renxia Zhang, Chunlai Jiang, Wei Kong, Yongge Wu
The combination of rabies immunoglobulin (RIG) with a vaccine is currently effective against rabies infections, but improvements are needed. Genetic engineering antibody technology is an attractive approach for developing novel antibodies to replace RIG. In our previous study, a single-chain variable fragment, scFv57R, against rabies virus glycoprotein (RVGP) was constructed. However, its inherent weak stability and short half-life compared with the parent RIG may limit its diagnostic and therapeutic application...
January 9, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27993967/an-fda-perspective-on-the-regulatory-implications-of-complex-signatures-to-predict-response-to-targeted-therapies
#3
Julia A Beaver, Abraham Tzou, Gideon M Blumenthal, Amy E McKee, Geoffrey Kim, Richard Pazdur, Reena Philip
As technologies evolve, and diagnostics move from detection of single biomarkers toward complex signatures, an increase in the clinical use and regulatory submission of complex signatures is anticipated. However, to date, no complex signatures have been approved as companion diagnostics. In this article, we will describe the potential benefit of complex signatures and their unique regulatory challenges including analytical performance validation, complex signature simulation, and clinical performance evaluation...
December 19, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27990088/impact-of-office-hysteroscopy-in-repeated-implantation-failure-experience-of-a-single-center
#4
Emre Göksan Pabuçcu, İbrahim Yalçın, Taylan Bodur, Gamze Sinem Çağlar, Recai Pabuçcu
OBJECTIVE: Repeated implantation failure (RIF) is a clinical entity affecting many couples undergoing assisted reproductive technology (ART). Various intrauterine pathologies contribute to RIF. Nevertheless, vaginal sonography and hysterosalpingography, which are the common diagnostic tools for the initial follow-up, have limited sensitivities. In this context, we aimed to evaluate the impact of office hysteroscopy (oHS) on live birth rates (LBRs) when performed prior to subsequent ART cycles in women with a history of RIF...
2016: Journal of the Turkish German Gynecological Association
https://www.readbyqxmd.com/read/27978590/laboratory-testing-for-von-willebrand-disease-the-past-present-and-future-state-of-play-for-von-willebrand-factor-assays-that-measure-platelet-binding-activity-with-or-without-ristocetin
#5
Sarah Just
von Willebrand disease (VWD) was first described nearly a century ago in 1924 by Erik Adolf von Willebrand. Diagnostic testing at the time was very limited and it was not until the mid to late 1900s that more tests became available to assist with the diagnosis and classification of VWD. Two of these tests are based on ristocetin, one being ristocetin-induced platelet aggregation (RIPA) and the other the von Willebrand factor (VWF) ristocetin cofactor assay (VWF:RCo). The VWF:RCo assay provides functional assessment of in vitro VWF binding to the platelet glycoprotein (Gp) complex, GPIb-IX-V...
December 15, 2016: Seminars in Thrombosis and Hemostasis
https://www.readbyqxmd.com/read/27965777/potential-of-molecular-based-diagnostics-and-its-impact-on-allergen-immunotherapy
#6
Giovanni Melioli, Eleonora Savi, Maria Angiola Crivellaro, Giovanni Passalacqua
Molecular based in vitro technologies greatly changed the diagnostic approaches in allergy. At present, sensitization profiles can be dissected according to IgE subsets, which are specific for genuine or cross-reacting components and potentially dangerous or virtually harmless components. The identification of IgE in components with specific characteristics has a direct impact on the accuracy of the diagnosis (indeed, it is possible at present to not only identify the allergen derived from a given allergen source but also the family of molecules to which the patient is sensitized), on the prognosis of the patient's allergy, and on the prevention activities to be implemented...
2016: Asthma research and practice
https://www.readbyqxmd.com/read/27933281/pet-imaging-of-urokinase-type-plasminogen-activator-receptor-upar-in-prostate-cancer-current-status-and-future-perspectives
#7
REVIEW
Dorthe Skovgaard, Morten Persson, Andreas Kjaer
Overexpression of urokinase-type plasminogen activator receptors (uPAR) represents an important biomarker for aggressiveness in most common malignant diseases, including prostate cancer (PC). Accordingly, uPAR expression either assessed directly in malignant PC tissue or assessed directly in plasma (intact/cleaved forms)-provides independent additional clinical information to that contributed by PSA, Gleason score, and other relevant pathological and clinical parameters. In this respect, non-invasive molecular imaging by positron emission tomography (PET) offers a very attractive technology platform, which can provide the required quantitative information on the uPAR expression profile, without the need for invasive procedures and the risk of missing the target due to tumor heterogeneity...
2016: Clinical and Translational Imaging: Reviews in Nuclear Medicine and Molecular Imaging
https://www.readbyqxmd.com/read/27932550/spermaurin-an-la1-like-peptide-from-the-venom-of-the-scorpion-scorpio-maurus-palmatus-improves-sperm-motility-and-fertilization-in-different-mammalian-species
#8
Guillaume Martinez, Jean-Pascal Hograindleur, Sébastien Voisin, Roland Abi Nahed, Tarek M Abd El Aziz, Jessica Escoffier, Julien Bessonnat, Claire-Maëlle Fovet, Michel De Waard, Sylviane Hennebicq, Vincent Aucagne, Pierre F Ray, Eric Schmitt, Philippe Bulet, Christophe Arnoult
STUDY QUESTION: Is it possible to identify original compounds that are able to enhance sperm motility from the venom of the scorpion Scorpio maurus palmatus? SUMMARY ANSWER: We identified a potent disulfide-rich peptide (DRP) of 73 amino acids that significantly improved the motility of fresh and frozen-thawed sperm in different mammalian species, including human, and improved fertilization outcome in mouse IVF experiments. WHAT IS KNOWN ALREADY: Any disturbance of sperm motility has a strong impact on fertilization and can lead to subfertility or infertility...
December 8, 2016: Molecular Human Reproduction
https://www.readbyqxmd.com/read/27924602/genome-wide-analysis-of-periodontal-and-peri-implant-cells-and-tissues
#9
Moritz Kebschull, Claudia Hülsmann, Per Hoffmann, Panos N Papapanou
Omics analyses, including the systematic cataloging of messenger RNA and microRNA sequences or DNA methylation patterns in a cell population, organ or tissue sample, are powerful means of generating comprehensive genome-level data sets on complex diseases. We have systematically assessed the transcriptome, miRNome and methylome of gingival tissues from subjects with different diagnostic entities of periodontal disease, and studied the transcriptome of primary cells ex vivo, or in vitro after infection with periodontal pathogens...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27918161/-current-options-of-preimplantion-genetic-screening-and-preimplantation-genetic-diagnostics
#10
V Šimečková
OBJECTIVE: The aim of this work is to summarize the current knowledge about preimplantation genetic screening and diagnostics. DESIGN: A review article. SETTING: Department of Gynecology and Obstetrics, District Hospital Šternberk, IVF Clinic, Olomouc. RESULTS: Preimplantation genetic testing is a complex of genetic and molecular cytogenetic examinations, which can help to detect abnormalities in embryos before transfer into the uterus of the mother...
2016: Ceská Gynekologie
https://www.readbyqxmd.com/read/27914804/nutrition-and-chronic-inflammatory-rheumatic-disease
#11
Luca Semerano, Chantal Julia, Ouidade Aitisha, Marie-Christophe Boissier
Nutrition is a major environmental influence on human health. Epidemiological and interventional studies suggest a pathophysiological or therapeutic role, respectively, for nutrition in inflammatory rheumatic diseases (IRDs). Nevertheless, the associations between nutrition and IRDs are often weak and inconsistent, and the available clinical trials on nutrition are methodologically flawed. Experimental evidence is accumulating that micronutrients in the diet may influence intestinal and systemic immune responses via complex interactions involving the gut microbiota...
November 30, 2016: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/27913044/clinical-validation-of-the-50-gene-ampliseq-cancer-panel-v2-for-use-on-a-next-generation-sequencing-platform-using-formalin-fixed-paraffin-embedded-and-fine-needle-aspiration-tumour-specimens
#12
Vivek Rathi, Gavin Wright, Diana Constantin, Siok Chang, Huong Pham, Kerryn Jones, Atha Palios, Sue-Anne Mclachlan, Matthew Conron, Penny McKelvie, Richard Williams
The advent of massively parallel sequencing has caused a paradigm shift in the ways cancer is treated, as personalised therapy becomes a reality. More and more laboratories are looking to introduce next generation sequencing (NGS) as a tool for mutational analysis, as this technology has many advantages compared to conventional platforms like Sanger sequencing. In Australia all massively parallel sequencing platforms are still considered in-house in vitro diagnostic tools by the National Association of Testing Authorities (NATA) and a comprehensive analytical validation of all assays, and not just mere verification, is a strict requirement before accreditation can be granted for clinical testing on these platforms...
January 2017: Pathology
https://www.readbyqxmd.com/read/27865995/g-quadruplex-based-aptamers-against-protein-targets-in-therapy-and-diagnostics
#13
REVIEW
Chiara Platella, Claudia Riccardi, Daniela Montesarchio, Giovanni N Roviello, Domenica Musumeci
Nucleic acid aptamers are single-stranded DNA or RNA molecules identified to recognize with high affinity specific targets including proteins, small molecules, ions, whole cells and even entire organisms, such as viruses or bacteria. They can be identified from combinatorial libraries of DNA or RNA oligonucleotides by SELEX technology, an in vitro iterative selection procedure consisting of binding (capture), partitioning and amplification steps. Remarkably, many of the aptamers selected against biologically relevant protein targets are G-rich sequences that can fold into stable G-quadruplex (G4) structures...
November 16, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27837499/commercial-molecular-tests-for-fungal-diagnosis-from-a-practical-point-of-view
#14
Michaela Lackner, Cornelia Lass-Flörl
The increasing interest in molecular diagnostics is a result of tremendously improved knowledge on fungal infections in the past 20 years and the rapid development of new methods, in particular polymerase chain reaction. High expectations have been placed on molecular diagnostics, and the number of laboratories now using the relevant technology is rapidly increasing-resulting in an obvious need for standardization and definition of laboratory organization. In the past 10 years, multiple new molecular tools were marketed for the detection of DNA, antibodies, cell wall components, or other antigens...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27836978/a-homozygous-nobox-truncating-variant-causes-defective-transcriptional-activation-and-leads-to-primary-ovarian-insufficiency
#15
Lin Li, Binbin Wang, Wei Zhang, Beili Chen, Minna Luo, Jing Wang, Xi Wang, Yunxia Cao, Kehkooi Kee
STUDY QUESTION: Does a novel homozygous NOBOX truncating variant, identified in whole exome sequencing (WES) of patients with primary ovarian insufficiency (POI), cause defective transcriptional activation of multiple oocyte-related genes? SUMMARY ANSWER: A novel homozygous truncating mutation of NOBOX was confirmed to exhibit a loss-of-function effect using well-defined molecular and functional analyses. WHAT IS KNOWN ALREADY: Several NOBOX mutations have been reported to be associated with POI but all of them are heterozygous mutations...
January 2017: Human Reproduction
https://www.readbyqxmd.com/read/27825889/carcinoembryonic-antigen-detection-with-handing-controlled-fluorescence-spectroscopy-using-a-color-matrix-for-point-of-care-applications
#16
Weijian Qin, Kan Wang, Kun Xiao, Yafei Hou, Wenting Lu, Hao Xu, Yan Wo, Shaoqing Feng, Daxiang Cui
In this study, we developed a power-free, accurate, and portable diagnostic platform called Handing based on embedded technology, which can rapidly detect fluorescent signals from quantum dots (QDs) on lateral flow test strips (LFTSs). The Handing system has three components: a hand-held test terminal, LFTS cartridge, and data server. The hand-held test terminal is the primary system component and it is integrated with tiny components using printed circuit board packaging for image acquisition, processing, and data handling by a specific program...
April 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27818705/fluorescent-nanoprobes-for-sensing-and-imaging-of-metal-ions-recent-advances-and-future-perspectives
#17
JingJing Zhang, FangFang Cheng, JingJing Li, Jun-Jie Zhu, Yi Lu
Recent advances in nanoscale science and technology have generated nanomaterials with unique optical properties. Over the past decade, numerous fluorescent nanoprobes have been developed for highly sensitive and selective sensing and imaging of metal ions, both in vitro and in vivo. In this review, we provide an overview of the recent development of the design and optical properties of the different classes of fluorescent nanoprobes based on noble metal nanomaterials, upconversion nanoparticles, semiconductor quantum dots, and carbon-based nanomaterials...
June 2016: Nano Today
https://www.readbyqxmd.com/read/27802166/cabyr-is-essential-for-fibrous-sheath-integrity-and-progressive-motility-in-mouse-spermatozoa
#18
Samantha A M Young, Haruhiko Miyata, Yuhkoh Satouh, Robert John Aitken, Mark A Baker, Masahito Ikawa
Ca(2+)-binding tyrosine-phosphorylation-regulated protein (CABYR) has been implicated in sperm physiological function in several in vitro studies. It has also been implicated as a potential cause of and diagnostic tool in asthenozoospermic human males. CABYR is known to be localized to the fibrous sheath, an accessory structure in the flagellar principal piece. Utilizing the CRISPR-Cas9 technology, we have knocked out this gene in mice to understand its role in male fertility. Cabyr-knockout male mice showed severe subfertility with a defect in sperm motility as well as a significant disorganization in the fibrous sheath...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27723157/improving-the-screening-of-blood-donors-with-syphilis-rapid-diagnostic-test-rdt-and-rapid-plasma-reagin-rpr-in-low-and-middle-income-countries-lmic
#19
F Sarkodie, O Hassall, E Owusu-Dabo, S Owusu-Ofori, I Bates, I C Bygbjerg, A Owusu-Ofori, L H Harritshøj, H Ullum
BACKGROUND: Syphilis testing conventionally relies on a combination of non-treponemal and treponemal tests. The primary objective of this study was to describe the positive predictive value (PPV) of a screening algorithm in a combination of a treponemal rapid diagnostic test (RDT) and rapid plasma reagin (RPR) test at Komfo Anokye Teaching Hospital (KATH), Ghana. MATERIALS AND METHODS: From February 2014 to January 2015, 5 mL of venous blood samples were taken from 16 016 blood donors and tested with a treponemal RDT; 5 mL of venous blood was taken from 526 consenting initial syphilis sero-reactive blood donors...
October 10, 2016: Transfusion Medicine
https://www.readbyqxmd.com/read/27713991/cmos-biosensors-for-in-vitro-diagnosis-transducing-mechanisms-and-applications
#20
Ka-Meng Lei, Pui-In Mak, Man-Kay Law, Rui P Martins
Complementary metal oxide semiconductor (CMOS) technology enables low-cost and large-scale integration of transistors and physical sensing materials on tiny chips (e.g., <1 cm(2)), seamlessly combining the two key functions of biosensors: transducing and signal processing. Recent CMOS biosensors unified different transducing mechanisms (impedance, fluorescence, and nuclear spin) and readout electronics have demonstrated competitive sensitivity for in vitro diagnosis, such as detection of DNA (down to 10 aM), protein (down to 10 fM), or bacteria/cells (single cell)...
September 21, 2016: Lab on a Chip
keyword
keyword
96970
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"