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In vitro diagnostic technology

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https://www.readbyqxmd.com/read/29928493/aptamers-novelty-tools-for-cancer-biology
#1
REVIEW
Ricardo L Pereira, Isis C Nascimento, Ana P Santos, Isabella E Y Ogusuku, Claudiana Lameu, Günter Mayer, Henning Ulrich
Although the term 'cancer' was still over two thousand years away of being coined, the first known cases of the disease date back to about 3000BC, in ancient Egypt. Five thousand years later, still lacking a cure, it has become one of the leading causes of death, killing over half a dozen million people yearly. So far, monoclonal antibodies are the most successful immune-therapy tools when it comes to fighting cancer. The number of clinical trials that use them has been increasing steadily during the past few years, especially since the Food and Drug Administration greenlit the use of the first immune-checkpoint blockade antibodies...
June 1, 2018: Oncotarget
https://www.readbyqxmd.com/read/29927355/horizon-2020-sme-instrument-topic-clinical-research-for-the-validation-of-biomarkers-and-or-diagnostic-medical-devices
#2
Jean-Luc Sanne
The European Commission released €130 million over 2014, 2015 and 2017 under the EU Framework Program for Research and Innovation, Horizon 2020, to support innovative small and medium-sized enterprises in the diagnostic area. The call topic focused on 'Clinical research for the validation of biomarkers and/or diagnostic medical devices'. It attracted 1194 applicants from all over Europe. The quality of the proposals was high and a large proportion of them were eligible for funding. In the majority, proposals were about in vitro diagnostics and tackled both clinical validation of new biomarkers and device optimization...
June 21, 2018: Personalized Medicine
https://www.readbyqxmd.com/read/29884677/current-and-emerging-trends-in-point-of-care-technology-and-strategies-for-clinical-validation-and-implementation
#3
REVIEW
Ping Wang, Larry J Kricka
BACKGROUND: Point-of-care technology (POCT)2 provides actionable information at the site of care to allow rapid clinical decision-making. With healthcare emphasis shifting toward precision medicine, population health, and chronic disease management, the potential impact of POCT continues to grow, and several prominent POCT trends have emerged or strengthened in the last decade. CONTENT: This review summarizes current and emerging trends in POCT, including technologies approved or cleared by the Food and Drug Administration or in development...
June 8, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29880747/landscape-phage-evolution-from-phage-display-to-nanobiotechnology
#4
REVIEW
Valery A Petrenko
The development of phage engineering technology has led to the construction of a novel type of phage display library-a collection of nanofiber materials with diverse molecular landscapes accommodated on the surface of phage particles. These new nanomaterials, called the "landscape phage", serve as a huge resource of diagnostic/detection probes and versatile construction materials for the preparation of phage-functionalized biosensors and phage-targeted nanomedicines. Landscape-phage-derived probes interact with biological threat agents and generate detectable signals as a part of robust and inexpensive molecular recognition interfaces introduced in mobile detection devices...
June 7, 2018: Viruses
https://www.readbyqxmd.com/read/29869625/microrna-708-3p-as-a-potential-therapeutic-target-via-the-adam17-gata-stat3-axis-in-idiopathic-pulmonary-fibrosis
#5
Bo Liu, Rongrong Li, Jinjin Zhang, Chao Meng, Jie Zhang, Xiaodong Song, Changjun Lv
MicroRNAs (miRNAs) are important diagnostic markers and therapeutic targets for many diseases. However, the miRNAs that control the pathogenesis of idiopathic pulmonary fibrosis (IPF) and act as potential therapeutic targets for the disease are rarely studied. In the present study, we analyzed the function and regulatory mechanism of microRNA-708-3p (miR-708-3p) and evaluated this marker's potential as a therapeutic target in IPF. The clinical and biological relevance of fibrogenesis for miR-708-3p was assessed in vivo and in vitro, specifically in matching plasma and tissue samples from 78 patients with IPF...
March 30, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29862965/the-future-for-diagnostic-tests-of-acute-kidney-injury-in-critical-care-evidence-synthesis-care-pathway-analysis-and-research-prioritisation
#6
Peter S Hall, Elizabeth D Mitchell, Alison F Smith, David A Cairns, Michael Messenger, Michelle Hutchinson, Judy Wright, Karen Vinall-Collier, Claire Corps, Patrick Hamilton, David Meads, Andrew Lewington
BACKGROUND: Acute kidney injury (AKI) is highly prevalent in hospital inpatient populations, leading to significant mortality and morbidity, reduced quality of life and high short- and long-term health-care costs for the NHS. New diagnostic tests may offer an earlier diagnosis or improved care, but evidence of benefit to patients and of value to the NHS is required before national adoption. OBJECTIVES: To evaluate the potential for AKI in vitro diagnostic tests to enhance the NHS care of patients admitted to the intensive care unit (ICU) and identify an efficient supporting research strategy...
May 2018: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/29862703/-contemporary-historical-development-and-analysis-of-in-vitro-diagnostics-ivd
#7
Jinghua Xing, Xunjun Zhang
By analyzing the contemporary key elements evolution of in vitro diagnostics (IVD), especially the rise in vitro diagnostic to the development of molecular diagnostics and precision medicine, from clinical laboratory to POCT, from basic research to the global regulation industry, this research is to expound the complex process and value of biomedical technology development and application.
November 30, 2017: Zhongguo Yi Liao Qi Xie za Zhi, Chinese Journal of Medical Instrumentation
https://www.readbyqxmd.com/read/29857831/3d-printed-tissue-engineered-model-for-bone-invasion-of-oral-cancer
#8
Thafar Almela, Sarmad Al-Sahaf, Ian M Brook, Kimia Khoshroo, Morteza Rasoulianboroujeni, Farahnaz Fahimipour, Mohammadreza Tahriri, Erfan Dashtimoghadam, Robert Bolt, Lobat Tayebi, Keyvan Moharamzadeh
Recent advances in three-dimensional printing technology have led to a rapid expansion of its applications in tissue engineering. The present study was designed to develop and characterize an in vitro multi-layered human alveolar bone, based on a 3D printed scaffold, combined with tissue engineered oral mucosal model. The objective was to incorporate oral squamous cell carcinoma (OSCC) cell line spheroids to the 3D model at different anatomical levels to represent different stages of oral cancer. Histological evaluation of the 3D tissue model revealed a tri-layered structure consisting of distinct epithelial, connective tissue, and bone layers; replicating normal oral tissue architecture...
June 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29700076/computer-aided-biochemical-programming-of-synthetic-microreactors-as-diagnostic-devices
#9
Alexis Courbet, Patrick Amar, François Fages, Eric Renard, Franck Molina
Biological systems have evolved efficient sensing and decision-making mechanisms to maximize fitness in changing molecular environments. Synthetic biologists have exploited these capabilities to engineer control on information and energy processing in living cells. While engineered organisms pose important technological and ethical challenges, de novo assembly of non-living biomolecular devices could offer promising avenues toward various real-world applications. However, assembling biochemical parts into functional information processing systems has remained challenging due to extensive multidimensional parameter spaces that must be sampled comprehensively in order to identify robust, specification compliant molecular implementations...
April 26, 2018: Molecular Systems Biology
https://www.readbyqxmd.com/read/29679865/generation-and-characterization-of-hagfish-variable-lymphocyte-receptor-b-against-glycoprotein-of-viral-hemorrhagic-septicemia-virus-vhsv
#10
Jung Seok Lee, Jaesung Kim, Se Pyeong Im, Si Won Kim, Jassy Mary S Lazarte, Jae Wook Jung, Tae Won Gong, Young Rim Kim, Jeong Ho Lee, Hyoung Jun Kim, Tae Sung Jung
Variable lymphocyte receptors B (VLRBs) are non-immunoglobulin components of the humoral immune system in jawless vertebrates including hagfish (Eptatretus burgeri) and lamprey (Petromyzon marinus). Hagfish VLRBs consist of leucine rich repeat (LRR) modules with a superhydrophobic C-terminal tail, the latter of which leads to extremely low expression levels in recombinant protein technology. Here, we present an artificially oligomerized VLRB (arVLRB) that conjugates via the C4bp oligomerization domain derived from human C4b-binding protein (hC4bp) rather than the superhydrophobic tail...
July 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29658818/a-long-non-coding-sineup-rna-boosts-semi-stable-production-of-fully-human-monoclonal-antibodies-in-hek293e-cells
#11
Emanuele Sasso, Debora Latino, Guendalina Froechlich, Mariangela Succoio, Margherita Passariello, Claudia De Lorenzo, Alfredo Nicosia, Nicola Zambrano
Use of monoclonal antibodies is emerging as a highly promising and fast-developing scenario for innovative treatment of viral, autoimmune and tumour diseases. The search for diagnostic and therapeutic antibodies currently depends on in vitro screening approaches, such as phage and yeast display technologies. Antibody production still represents a critical step for preclinical and clinical evaluations. Accordingly, improving production of monoclonal antibodies represents an opportunity, to facilitate downstream target validations...
April 16, 2018: MAbs
https://www.readbyqxmd.com/read/29618499/comparison-of-an-in-vitro-diagnostic-next-generation-sequencing-assay-with-sanger-sequencing-for-hiv-1-genotypic-resistance-testing
#12
Philip L Tzou, Pramila Ariyaratne, Vici Varghese, Charlie Lee, Elian Rakhmanaliev, Carolin Villy, Meiqi Yee, Kevin Tan, Gerd Michel, Benjamin A Pinsky, Robert W Shafer
The ability of next-generation sequencing (NGS) technologies to detect low frequency HIV-1 drug resistance mutations (DRMs) not detected by dideoxynucleotide Sanger sequencing has potential advantages for improved patient outcomes. We compared the performance of an in vitro diagnostic (IVD) NGS assay, the Sentosa SQ HIV genotyping assay for HIV-1 genotypic resistance testing, with Sanger sequencing on 138 protease/reverse transcriptase (RT) and 39 integrase sequences. The NGS assay used a 5% threshold for reporting low-frequency variants...
June 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29616985/recording-characteristics-of-electrical-impedance-electromyography-needle-electrodes
#13
H Kwon, J F Di Cristina, S B Rutkove, B Sanchez
OBJECTIVE: Needle EMG remains the standard clinical test for neuromuscular disease (NMD) assessment, but it only characterizes myofiber membrane depolarization. On the other hand, electrical impedance provides non-electrically active structural and compositional data of tissues. Here, we designed a prototype of needle electrode integrating electrical impedance and EMG measurement capabilities, the so-called I-EMG needle electrode. APPROACH: We use finite element method models to study the impedance recording characteristics of I-EMG needle electrodes...
May 22, 2018: Physiological Measurement
https://www.readbyqxmd.com/read/29577645/a-novel-monoclonal-antibody-kmp1-has-potential-antitumor-activity-of-bladder-cancer-by-blocking-cd44-in-vivo-and-in-vitro
#14
Yujin Chen, Haifeng Wang, Yigang Zuo, Ning Li, Mingxia Ding, Chong Li
Bladder cancer becomes a serious medical and social concern due to its high recurrence and mortality rates. Thus, it is urgent to search a novel prognostic biomarker and targeted therapy with high sensitivity and specificity. In this study, we used the human bladder cancer cell line EJ as an immunogen to generate a novel mouse monoclonal antibody KMP1 that specifically bound to bladder cancer, and then, the antitumor effect of KMP1 against bladder cancer was investigated both in vivo and in vitro. The results showed that expression of the KMP1 epitope is consistent with clinical severity and prognosis of bladder cancer...
May 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29566378/adrenal-insufficiency-sex-reversal-and-angelman-syndrome-due-to-uniparental-disomy-unmasking-a-mutation-in-cyp11a1
#15
Ahlee Kim, Masanobu Fujimoto, Vivian Hwa, Philippe Backeljauw, Andrew Dauber
BACKGROUND/AIMS: Cholesterol side-chain cleavage enzyme (P450scc) deficiency is a rare genetic disorder causing primary adrenal insufficiency with or without a 46,XY disorder of sexual development (DSD). Herein, we report a case of the combination of primary adrenal insufficiency, a DSD (testes with female external genitalia in a setting of a 47,XXY karyotype), and Angelman syndrome. METHODS: Comprehensive genetic analyses were performed, including a single nucleotide polymorphism microarray and whole-exome sequencing...
2018: Hormone Research in Pædiatrics
https://www.readbyqxmd.com/read/29561796/disease-modeling-using-3d-organoids-derived-from-human-induced-pluripotent-stem-cells
#16
REVIEW
Beatrice Xuan Ho, Nicole Min Qian Pek, Boon-Seng Soh
The rising interest in human induced pluripotent stem cell (hiPSC)-derived organoid culture has stemmed from the manipulation of various combinations of directed multi-lineage differentiation and morphogenetic processes that mimic organogenesis. Organoids are three-dimensional (3D) structures that are comprised of multiple cell types, self-organized to recapitulate embryonic and tissue development in vitro. This model has been shown to be superior to conventional two-dimensional (2D) cell culture methods in mirroring functionality, architecture, and geometric features of tissues seen in vivo...
March 21, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29536992/mobile-diagnostics-next-generation-technologies-for-in-vitro-diagnostics
#17
Joonchul Shin, Sudesna Chakravarty, Wooseok Choi, Kyungyeon Lee, Dongsik Han, Hyundoo Hwang, Jaekyu Choi, Hyo-Il Jung
The emergence of a wide range of applications of smartphones along with advances in 'liquid biopsy' has significantly propelled medical research particularly in the field of in vitro diagnostics (IVD). Herein, we have presented a detailed analysis of IVD, its associated critical concerns and probable solutions. It also demonstrates the transition in terms of analytes from minimally invasive (blood) to non-invasive (urine, saliva and sweat) and depicts how the different features of a smartphone can be integrated for specific diagnostic purposes...
March 26, 2018: Analyst
https://www.readbyqxmd.com/read/29509709/cell-free-approaches-in-synthetic-biology-utilizing-microfluidics
#18
REVIEW
Samar Damiati, Rami Mhanna, Rimantas Kodzius, Eva-Kathrin Ehmoser
Synthetic biology is a rapidly growing multidisciplinary branch of science which aims to mimic complex biological systems by creating similar forms. Constructing an artificial system requires optimization at the gene and protein levels to allow the formation of entire biological pathways. Advances in cell-free synthetic biology have helped in discovering new genes, proteins, and pathways bypassing the complexity of the complex pathway interactions in living cells. Furthermore, this method is cost- and time-effective with access to the cellular protein factory without the membrane boundaries...
March 6, 2018: Genes
https://www.readbyqxmd.com/read/29504623/clinical-applications-of-infrared-and-raman-spectroscopy-state-of-play-and-future-challenges
#19
REVIEW
Matthew J Baker, Hugh J Byrne, John Chalmers, Peter Gardner, Royston Goodacre, Alex Henderson, Sergei G Kazarian, Francis L Martin, Julian Moger, Nick Stone, Josep Sulé-Suso
Vibrational spectroscopies, based on infrared absorption and/or Raman scattering provide a detailed fingerprint of a material, based on the chemical content. Diagnostic and prognostic tools based on these technologies have the potential to revolutionise our clinical systems leading to improved patient outcome, more efficient public services and significant economic savings. However, despite these strong drivers, there are many fundamental scientific and technological challenges which have limited the implementation of this technology in the clinical arena, although recent years have seen significant progress in addressing these challenges...
April 16, 2018: Analyst
https://www.readbyqxmd.com/read/29474672/leptomeninges-derived-induced-pluripotent-stem-cells-and-directly-converted-neurons-from-autopsy-cases-with-varying-neuropathologic-backgrounds
#20
Shannon E Rose, Harald Frankowski, Allison Knupp, Bonnie J Berry, Refugio Martinez, Stephanie Q Dinh, Lauren T Bruner, Sherry L Willis, Paul K Crane, Eric B Larson, Thomas Grabowski, Martin Darvas, C Dirk Keene, Jessica E Young
Patient-specific stem cell technology from skin and other biopsy sources has transformed in vitro models of neurodegenerative disease, permitting interrogation of the effects of complex human genetics on neurotoxicity. However, the neuropathologic changes that underlie cognitive and behavioral phenotypes can only be determined at autopsy. To better correlate the biology of derived neurons with age-related and neurodegenerative changes, we generated leptomeningeal cell lines from well-characterized research subjects that have undergone comprehensive postmortem neuropathologic examinations...
May 1, 2018: Journal of Neuropathology and Experimental Neurology
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