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https://www.readbyqxmd.com/read/28650789/effects-of-cola-flavored-beverages-and-caffeine-on-streptococcus-mutans-biofilm-formation-and-metabolic-activity
#1
Roger P Dotsey, Elizabeth A S Moser, George J Eckert, Richard L Gregory
OBJECTIVE: To examine the effects of cola-flavored beverages and caffeine on growth and metabolism of Streptococcus mutans biofilm. This study was designed to determine if carbonated beverages or caffeine can increase S. mutans growth and biofilm formation and metabolic activity in vitro, potentially leading to increased S. mutans-associated cariogenicity in children that consume them. STUDY DESIGN: Six different cola-flavored products, plus pure caffeine, and pure high fructose corn syrup (HFCS), at different concentrations similar to those in the beverages were tested...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28649961/optoelectronic-device-physics-and-technology-of-nitride-semiconductors-from-the-uv-to-the-terahertz-a-review
#2
Theodore D Moustakas, Roberto Paiella
This paper reviews the device physics and technology of optoelectronic devices based on semiconductors of the GaN family, operating in the spectral regions from deep UV to Terahertz. Such devices include LEDs, lasers, detectors, electroabsorption modulators and devices based on intersubband transitions in AlGaN quantum wells (QWs). After a brief history of the development of the field, we describe how the unique crystal structure, chemical bonding, and resulting spontaneous and piezoelectric polarizations in heterostructures affect the design, fabrication and performance of devices based on these materials...
June 26, 2017: Reports on Progress in Physics
https://www.readbyqxmd.com/read/28648453/tolerance-of-pseudomonas-aeruginosa-in-in-vitro-biofilms-to-high-level-peracetic-acid-disinfection
#3
Ayorinde Akinbobola, Leighann Sherry, William G Mckay, Gordon Ramage, Craig Williams
BACKGROUND: Biofilm has been suggested as a cause of disinfection failures in flexible endoscopes where no lapses in the decontamination procedure can be identified. To test this theory, the activity of peracetic acid (PAA), one of the commonly used disinfectants in the reprocessing of flexible endoscopes, was evaluated against both planktonic and sessile communities of Pseudomonas aeruginosa. AIM: To investigate the ability of P. aeruginosa biofilm to survive high level PAA disinfection...
June 22, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28645294/3-5-4-trihydroxy-6-7-3-trimethoxyflavone-protects-against-beta-amyloid-induced-neurotoxicity-through-antioxidative-activity-and-interference-with-cell-signaling
#4
Alona Telerman, Rivka Ofir, Yoel Kashman, Anat Elmann
BACKGROUND: Alzheimer's disease is a neurodegenerative disease, characterized by progressive decline in memory and cognitive functions, that results from loss of neurons in the brain. Amyloid beta (Aβ) protein and oxidative stress are major contributors to Alzheimer's disease, therefore, protecting neuronal cells against Aβ-induced toxicity and oxidative stress might form an effective approach for treatment of this disease. 3,5,4'-trihydroxy-6,7,3'-trimethoxyflavone (TTF) is a flavonoid we have purified from the plant Achillea fragrantissima; and the present study examined, for the first time, the effects of this compound on Aβ-toxicity to neuronal cells...
June 23, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28633820/green-synthesis-of-ag-cr-ac-nanocomposites-by-azadirachta-indica-and-its-application-for-the-simultaneous-removal-of-binary-mixture-of-dyes-by-ultrasonicated-assisted-adsorption-process-using-response-surface-methodology
#5
Muhammad Saad, Hajira Tahir, Duaa Ali
In the present studies the Ag-Cr-AC nanocomposites were synthesized by Azadirachta indica leaves extract. They were inoculated on the amorphous surface of activated carbon. The surface morphology and structural identification was determined by SEM, FTIR and XRD techniques. The simultaneous removal of binary dye system of Reactive Red and Crystal Violet were performed by ultrasonicated assisted adsorption process utilizing Ag-Cr-AC nanocomposites. Central Composite Design (CCD) having 5 factors of time, pH, amount of Ag-Cr-AC (adsorbent), concentrations of Reactive Red (RR) and Crystal Violet (CV) was employed...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28632948/nodular-cutaneous-amyloidoma-of-the-extremity-secondary-to-chronic-granulomatous-inflammation-in-setting-of-sarcoidosis
#6
Victoria Billero, Audrey A Jacobsen, Mariya I Miteva, Adam J Wulkan, Claudio Marasca, Roanelli Paolo
Soft tissue amyloidoma is a rare condition that presents primarily in the abdomen and/or mediastinum and more uncommonly on the extremities(1) . Soft tissue amyloidomas on the extremities have been associated with chronic inflammation, particularly when accompanied by AA-type amyloid deposition as seen in local trauma, surgery, hypertension, and diabetes(2) . To our knowledge, this is the first reported case of nodular cutaneous amyloidoma in the setting of systemic and cutaneous sarcoidosis. A 65-year-old woman presented with an asymptomatic subcutaneous nodule above her left lateral malleolus...
June 20, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28629363/fibroblast-paracrine-tnf-%C3%AE-signaling-elevates-integrin-a5-expression-in-idiopathic-pulmonary-fibrosis-ipf
#7
Gali Epstein Shochet, Elizabetha Brook, Lilach Israeli-Shani, Evgeny Edelstein, David Shitrit
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a progressive lung disease with a poor prognosis. Inflammatory cytokines play a significant role in IPF pathology. However, the fibroblast itself is also believed to be the primary effector in IPF. We hypothesized that the fibroblasts themselves secrete pro-inflammatory cytokines that could propagate IPF by affecting normal neighboring cells. Thus, we explored the effects of IPF fibroblast derived media on normal fibroblast characteristics...
June 19, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28622484/bactericidal-effect-of-er-yag-laser-activated-sodium-hypochlorite-irrigation-against-biofilms-of-enterococcus-faecalis-isolate-from-canal-of-root-filled-teeth-with-periapical-lesions
#8
Xiaogang Cheng, Doudou Xiang, Wenxi He, Jun Qiu, Bing Han, Qing Yu, Yu Tian
OBJECTIVE: This study was to evaluate the bactericidal effect of Er:YAG laser-activated sodium hypochlorite irrigation (Er:YAG + NaOCl) on biofilms of Enterococcus faecalis clinical isolate. BACKGROUND DATA: It was reported that Er:YAG + NaOCl had effective bactericidal effect on laboratory-adapted E. faecalis strain, while no study has reported its effect on the clinical isolate. METHODS: Eighteen E. faecalis strains were isolated from 39 root-filled teeth with periapical lesions, and their biofilm formation abilities were evaluated using the crystal violet staining method...
June 16, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28618365/a-highly-sensitive-and-widely-adaptable-plasmonic-aptasensor-using-berberine-for-small-molecule-detection
#9
Jin-Ho Park, Ju-Young Byun, Hyungjun Jang, Donggu Hong, Min-Gon Kim
Localized surface plasmon resonance (LSPR) biosensors allow label-free detection of small molecules in molecular binding events; however, they are limited by a relatively low sensitivity and narrow dynamic range. Here, we report highly sensitive small-molecule detection by LSPR peak shift exploiting the G-quadruplex (GQx) structure-binding characteristic of known GQx binders to enhance the LSPR signal of a plasmonic aptasensor. Six known GQx binders (thiazole orange, malachite green, crystal violet, zinc protoporphyrin IX, thioflavin T, and berberine) were tested for their ability to enhance the LSPR signal...
June 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28618020/bacterial-colonization-of-resin-composite-cements-influence-of-material-composition-and-surface-roughness
#10
Stephanie Glauser, Monika Astasov-Frauenhoffer, Johannes A Müller, Jens Fischer, Tuomas Waltimo, Nadja Rohr
So-called secondary caries may develop in the cement gap between the tooth and the bonded restoration. Cement materials with a low susceptibility to biofilm formation are therefore desirable. In the present study, the adhesion of Strepococcus mutans onto three adhesive (Multilink Automix, RelyX Ultimate, and Panavia V5) and three self-adhesive (Multilink Speed Cem, RelyX Unicem 2 Automix, and Panavia SA plus) resin composite cements was evaluated. Previous studies have failed to evaluate concomitantly the effect of both the composition of the cements and their surface roughness on biofilm formation...
June 15, 2017: European Journal of Oral Sciences
https://www.readbyqxmd.com/read/28616631/the-effect-of-emodin-on-staphylococcus-aureus-strains-in-planktonic-form-and-biofilm-formation-in-vitro
#11
Xin Yan, Shanshan Gu, Yunjia Shi, Xingyang Cui, Shanshan Wen, Junwei Ge
Staphylococcus aureus (S. aureus) is a Gram-positive pathogen and forms biofilm easily. Bacteria inside biofilms display an increased resistance to antibiotics and disinfectants. The objective of the current study was to assess the antimicrobial activities of emodin, 1,2,8-trihydroxy-6-methylanthraquinone, an anthraquinone derivative isolated from Polygonum cuspidatum and Rheum palmatum, against S. aureus CMCC26003 grown in planktonic and biofilm cultures in vitro. In addition, a possible synergistic effect between emodin and berberine chloride was evaluated...
June 14, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28608317/ica-status-of-clinical-staphylococcus-epidermidis-strains-affects-adhesion-and-aggregation-a-thermodynamic-analysis
#12
Titik Nuryastuti, Bastiaan P Krom
Staphylococcus epidermidis is a major nosocomial pathogen associated with infections of indwelling medical devices. One important virulence factor of these organisms is their ability to adhere to devices and form biofilms. In this study, we evaluated the effect of the ica operon on cell surface hydrophobicity, thermodynamics of adhesion, and biofilm formation for seven S. epidermidis strains. The surface free energy parameters of the bacterial cell surface and the substratum were determined by contact angle measurement...
June 12, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28603699/crispr-interference-crispri-inhibition-of-luxs-gene-expression-in-e-coli-an-approach-to-inhibit-biofilm
#13
Azna Zuberi, Lama Misba, Asad U Khan
Biofilm is a sessile bacterial accretion embedded in self-producing matrix. It is the root cause of about 80% microbial infections in human. Among them, E. coli biofilms are most prevalent in medical devices associated nosocomial infections. The objective of this study was to inhibit biofilm formation by targeting gene involved in quorum sensing, one of the main mechanisms of biofilm formation. Hence we have introduced the CRISPRi, first time to target luxS gene. luxS is a synthase, involved in the synthesis of Autoinducer-2(AI-2), which in turn guides the initial stage of biofilm formation...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28602275/alcohol-fixation-of-bacteria-to-surgical-instruments-increases-cleaning-difficulty-and-may-contribute-to-sterilization-inefficacy
#14
Dayane de Melo Costa, Lillian Kelly de Oliveira Lopes, Honghua Hu, Anaclara Ferreira Veiga Tipple, Karen Vickery
BACKGROUND: Alcohol is frequently inappropriately used on surgical instruments to reduce bacterial contamination, but fixes protein to stainless steel. Here we compare the effect of air drying, prolonged soaking in water, and alcohol treatment on cleaning difficulty of contaminated forceps. METHODS: Haltsted-mosquito forceps were contaminated with only Staphylococcus aureus. Instruments were air-dried for 10 (control), 75, or 240 minutes, soaked in water, or air dried then treated with ethanol or isopropanol for 10 seconds...
June 8, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28599174/persistent-listeria-monocytogenes-strains-isolated-from-mussel-production-facilities-form-more-biofilm-but-are-not-linked-to-specific-genetic-markers
#15
Jessika Nowak, Cristina D Cruz, Marcel Tempelaars, Tjakko Abee, Arnoud H M van Vliet, Graham C Fletcher, Duncan Hedderley, Jon Palmer, Steve Flint
Contamination of mussels with the human pathogen Listeria monocytogenes occurs during processing in the factory, possibly from bacteria persisting in the factory's indoor and outdoor areas. In this study, a selection of persistent (n=8) and sporadic (n=8) L. monocytogenes isolates associated with mussel-processing premises in New Zealand were investigated for their phenotypic and genomic characteristics. To identify traits that favour or contribute to bacterial persistence, biofilm formation, heat resistance, motility and recovery from dry surfaces were compared between persistent and sporadic isolates...
June 1, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28595275/antifungal-susceptibility-of-sporothrix-schenckii-complex-biofilms
#16
Raimunda Sâmia Nogueira Brilhante, Felipe Rodrigues Magalhães de Aguiar, Maria Lucilene Queiroz da Silva, Jonathas Sales de Oliveira, Zoilo Pires de Camargo, Anderson Messias Rodrigues, Vandbergue Santos Pereira, Rosana Serpa, Débora de Souza Collares Maia Castelo-Branco, Edmilson Emanuel Monteiro Correia, Waldemiro Aquino Pereira-Neto, Rossana de Aguiar Cordeiro, Marcos Fábio Gadelha Rocha, José Júlio Costa Sidrim
Sporotrichosis, caused by species of Sporothrix schenckii complex, is the most prevalent subcutaneous mycosis in many areas of Latin America. The aim of this study was to evaluate the ability of Sporothrix spp. to form biofilms in vitro and to characterize the growth kinetics, morphology, and antifungal susceptibility of biofilms against classical antifungals. We investigated the ability of strains to produce biofilms in vitro and determined the effects of exposure to amphotericin B, itraconazole, caspofungin, ketoconazole, voriconazole, and fluconazole at minimum inhibitory concentration (MIC) against planktonic form and at 10× MIC and 50× MIC on the biomass and metabolic activity of these biofilms...
June 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28594946/association-of-vaginal-dysbiosis-and-biofilm-with-contraceptive-vaginal-ring-biomass-in-african-women
#17
Liselotte Hardy, Vicky Jespers, Irith De Baetselier, Jozefien Buyze, Lambert Mwambarangwe, Viateur Musengamana, Janneke van de Wijgert, Tania Crucitti
We investigated the presence, density and bacterial composition of contraceptive vaginal ring biomass and its association with the vaginal microbiome. Of 415 rings worn by 120 Rwandese women for three weeks, the biomass density was assessed with crystal violet and the bacterial composition of biomass eluates was assessed with quantitative polymerase chain reaction (qPCR). The biomass was visualised after fluorescence in situ hybridisation (FISH) and with scanning electron microscopy (SEM). The vaginal microbiome was assessed with Nugent scoring and vaginal biofilm was visualised after FISH...
2017: PloS One
https://www.readbyqxmd.com/read/28591948/-the-role-of-hmgn2-in-the-development-of-periodontitis-dental-plaque
#18
Yang Luo, Qian Li, Ya-Ping Fan, Jiao Chen, Ping Zhang, Yun Feng
OBJECTIVES: To determine the role of high mobility group chromosomal protein N2 (HMGN2) in the development of periodontitis plaque biofilm. METHODS: Saliva samples were collected before clinical interventions in patients with periodontitis (25 Mild periodontitis, 25 Moderate periodontitis and 25 Severe periodontitis) and healthy controls ( n=25).Following an estimation of dental plaque index, the level of HMGN2 in saliva was determined by enzyme-linked immunosorbent assay (ELISA)...
July 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28585714/use-of-standing-gold-nanorods-for-detection-of-malachite-green-and-crystal-violet-in-fish-by-sers
#19
Xiaowei Chen, Trang H D Nguyen, Liqun Gu, Mengshi Lin
With growing consumption of aquaculture products, there is increasing demand on rapid and sensitive techniques that can detect prohibited substances in the seafood products. This study aimed to develop a novel surface-enhanced Raman spectroscopy (SERS) method coupled with simplified extraction protocol and novel gold nanorod (AuNR) substrates to detect banned aquaculture substances (malachite green [MG] and crystal violet [CV]) and their mixture (1:1) in aqueous solution and fish samples. Multivariate statistical tools such as principal component analysis (PCA) and partial least squares regression (PLSR) were used in data analysis...
June 6, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28567055/surface-characteristics-and-biofilm-development-on-selected-dental-ceramic-materials
#20
Kyoung H Kim, Carolina Loch, J Neil Waddell, Geoffrey Tompkins, Donald Schwass
BACKGROUND: Intraoral adjustment and polishing of dental ceramics often affect their surface characteristics, promoting increased roughness and consequent biofilm growth. This study correlated surface roughness to biofilm development with four commercially available ceramic materials. METHODS: Four ceramic materials (Vita Enamic®, Lava™ Ultimate, Vitablocs Mark II, and Wieland Reflex®) were prepared as per manufacturer instructions. Seventeen specimens of each material were adjusted and polished to simulate clinical intraoral procedures and another seventeen remained unaltered...
2017: International Journal of Dentistry
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