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crystal violet

Takeaki Wajima, Yui Anzai, Tetsuya Yamada, Hideaki Ikoshi, Norihisa Noguchi
Oldenlandia diffusa has been empirically used as a therapeutic adjunct for the treatment of respiratory infections. To establish the basic evidence of its clinical usefulness, antimicrobial and biofilm inhibitory activities of an O. diffusa extract were examined against clinical isolates of Haemophilus influenzae, a major causative pathogen of respiratory and sensory organ infections. No significant growth inhibitory activity was observed during incubation for more than 6 h after the extract addition into a culture of H...
2016: PloS One
Celha Borges Costa Alves, Márcio Nunes Segurado, Miriam Cristina Leandro Dorta, Fátima Ribeiro Dias, Maurício Guilherme Lenza, Marcos Augusto Lenza
Objective: To evaluate and compare in vitro cytotoxicity and corrosion resistance of mini-implants from three different commercial brands used for orthodontic anchorage. Methods: Six mini-implants (Conexão(tm), Neodent(tm) and SIN(tm)) were separately immersed in artificial saliva (pH 6.76) for 30 and 60 days. The cytotoxicity of the corrosion extracts was assessed in L929 cell cultures using the violet crystal and MTT assays, as well as cell morphology under light microscopy...
September 2016: Dental Press Journal of Orthodontics
Alexey A Sysoev, Sergey S Poteshin, Denis M Chernyshev, Alexander A Sysoev
An ion mobility time-of-flight mass spectrometry (IM-TOFMS)-based method has been preliminarily investigated for the identification of triphenylmethane ballpoint pen dyes on paper. The dyes were sampled from one-year-old ballpoint pen ink entries. The entries were written on paper documents stored in the dark in a bookcase. Sample solutions were prepared by extraction of dyes in a vial. Basic violet 2, Methyl violet 6B, Methyl violet 2B and Crystal violet were characterized by IM-TOFMS. Since the ballpoint ink dyes contain ionic compounds, the studied compounds were expected to form stable peaks in the atmospheric pressure drift tube ion mobility spectrometry, and this was experimentally verified...
2016: European Journal of Mass Spectrometry
Ankita Tandon, Narendra Nath Singh, V R Brave, Gadiputi Sreedhar
BACKGROUND: Mitosis is a process of cell division resulting in two genetically equivalent daughter cells. Excessive proliferation of cells due to mitosis is the hallmark in pre cancer and cancer. AIMS: This study was conducted to count the number of mitotic figures in normal oral mucosa, oral epithelial dysplasia and squamous cell carcinoma in both Hematoxylin and Eosin (H&E) and Crystal Violet stained sections. Also the overall number of mitotic figures with both stains were compared along with the evaluation of staining efficacy of both the stains...
November 2016: Journal of Oral Biology and Craniofacial Research
D Knafl, S Tobudic, S C Cheng, D R Bellamy, F Thalhammer
Activity of dalbavancin against methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus epidermidis (MRSE) in biofilm was investigated and the microbicidal biofilm concentrations (MBC) were determined. Biofilms obtained from ten MRSA and ten MRSE bloodstream isolates, collected from patients in the General Hospital of Vienna between 2012 and 2015, were incubated with dalbavancin in trypticase soy broth (TSB) in serial dilution from 0.0625 mg/l to 256 mg/l using a microtiter plate biofilm model...
November 28, 2016: European Journal of Clinical Microbiology & Infectious Diseases
H Jayasantha Kumari, P Krishnamoorthy, T K Arumugam, S Radhakrishnan, D Vasudevan
A composite of Typha latifolia activated carbon (TLAC) (a novel, low cost absorbent) and chitosan (TLAC/Chitosan composite) was prepared. The composite was characterised using IR spectra, XRD, FESEM and Pore size studies. Its effectivity was tested for the removal of crystal violet dye from aqueous solutions. The effect of pH, dose rate and initial dye concentration was evaluated. The adsorption isotherm, kinetics and thermodynamic parameters were studied. Langmuir and Freundlich isotherm models were found fit effectively for the dye adsorption data in the present study...
November 23, 2016: International Journal of Biological Macromolecules
Francesca Uberti, Claudio Bardelli, Vera Morsanuto, Sabrina Ghirlanda, Andrea Cochis, Claudio Molinari
The physiological effects of acetylcholine on keratinocytes depend on the presence of nicotinic and muscarinic receptors. The role of nonneuronal acetylcholine in keratinocytes could have important clinical implications for patients with various skin disorders such as nonhealing wounds. In order to evaluate the efficacy of highly diluted acetylcholine solutions obtained by sequential kinetic activation, we aimed to investigate the effects of these solutions on normal human keratinocytes. Two different concentrations (10 fg/mL and 1 pg/mL) and formulations (kinetically activated and nonkinetically activated) of acetylcholine were used to verify keratinocyte viability, proliferation, and migration and the intracellular pathways involved using MTT, crystal violet, wound healing, and Western blot compared to 147 ng/mL acetylcholine...
November 25, 2016: Cells, Tissues, Organs
Jiayin Sun, J Frederique Comeau, John E Baenziger
In the absence of activating anionic lipids and cholesterol, the nicotinic acetylcholine receptor (nAChR) from Torpedo adopts an uncoupled conformation that does not usually gate open in response to agonist. The uncoupled conformation binds both agonists and non-competitive channel blockers with a lower affinity than the desensitized state, consistent with both the extracellular agonist-binding and transmembrane channel-gating domains individually adopting resting-state like conformations. To test this hypothesis, we characterized the binding of the agonist, acetylcholine, and two fluorescent channel blockers, ethidium and crystal violet, to resting, desensitized and uncoupled nAChRs in reconstituted membranes...
November 19, 2016: Biochimica et Biophysica Acta
Eric Hong Jian Wong, Chow Goon Ng, Eng Guan Chua, Alfred Chin Yen Tay, Fanny Peters, Barry J Marshall, Bow Ho, Khean Lee Goh, Jamuna Vadivelu, Mun Fai Loke
BACKGROUND: Biofilm formation by Helicobacter pylori may be one of the factors influencing eradication outcome. However, genetic differences between good and poor biofilm forming strains have not been studied. MATERIALS AND METHODS: Biofilm yield of 32 Helicobacter pylori strains (standard strain and 31 clinical strains) were determined by crystal-violet assay and grouped into poor, moderate and good biofilm forming groups. Whole genome sequencing of these 32 clinical strains was performed on the Illumina MiSeq platform...
2016: PloS One
Elise Melloul, Stéphanie Luiggi, Leslie Anaïs, Pascal Arné, Jean-Marc Costa, Vincent Fihman, Benoit Briard, Eric Dannaoui, Jacques Guillot, Jean-Winoc Decousser, Anne Beauvais, Françoise Botterel
BACKGROUND: Biofilms are communal structures of microorganisms that have long been associated with a variety of persistent infections poorly responding to conventional antibiotic or antifungal therapy. Aspergillus fumigatus fungus and Stenotrophomonas maltophilia bacteria are examples of the microorganisms that can coexist to form a biofilm especially in the respiratory tract of immunocompromised patients or cystic fibrosis patients. The aim of the present study was to develop and assess an in vitro model of a mixed biofilm associating S...
2016: PloS One
Seung-Gyun Woo, So-Yeon Lee, So-Min Lee, Kyoung-Hee Lim, Eun-Ju Ha, Yong-Bin Eom
Staphylococcus aureus is one of the most important pathogens causing chronic biofilm infections. These are becoming more difficult to treat owing to drug resistance, particularly because S. aureus biofilms limit the efficacy of antimicrobial agents, leading to high morbidity and mortality. In the present study, we screened for inhibitors of S. aureus biofilm formation using a natural product library from the Korea Chemical Bank (KCB). Screening by crystal violet-based biomass staining assay identified hit compounds...
November 18, 2016: Folia Microbiologica
C H Sudhakar Reddy, G Ravi, K Sreenu, Ravinder Guje, M Malathi, Muga Vithal
Phosphates as multi-functional materials were of vital importance in the environmental and energy fields. In the present work, a new cyclophosphate, potassium-manganese-phosphate (KMnP3 O9 ) (hereafter KMPO), was prepared by solid state method. Cations (Ag(+) and Cu(2+) ) and anion (N(3-) ) were substituted into KMPO lattice via ion-exchange and solid state methods respectively. The as-prepared materials were characterized by powder X-ray diffraction, SEM-EDS and UV-visible diffuse reflectance spectra. Rietveld refinement was carried out for parent material...
November 18, 2016: Photochemistry and Photobiology
Yuri A Rulev, Zalina T Gugkaeva, Anastasia V Lokutova, Victor I Maleev, Alexander S Peregudov, Xiao Wu, Michael North, Yuri N Belokon
Carbocation/polyol systems are shown to be highly efficient catalysts for the synthesis of cyclic carbonates from epoxides and carbon dioxide at 50 °C and 5 MPa CO2 pressure. The best activity was shown by the combination of crystal violet and 1,1'-bi-2-naphthol (BINOL), which could be recycled five times with no loss of activity. The presence of specific interactions between the amino groups of the carbocation and the hydroxyl protons was confirmed by NMR experiments. The Job plots for the crystal violet iodide/BINOL and brilliant green iodide/BINOL systems showed that the catalytic systems consist of one molecule of the carbocation and one molecule of BINOL...
November 16, 2016: ChemSusChem
Zaixiang Lou, Hongxin Wang, Yuxia Tang
AIMS: This study aimed to evaluate the effect of a fraction of burdock (Arctium lappa L.) leaf on the initial adhesion, biofilm formation, quorum sensing and virulence factors of P. aeruginosa. METHODS AND RESULTS: Anti-biofilm activity of the burdock leaf fraction was studied by the method of crystal violet staining. When the concentration of the burdock leaf fraction was 2.0 mg mL(-1) , the inhibition rates on biofilm formation of P. aeruginosa were 100%. The burdock leaf fraction was found to inhibit the formation of biofilm by reducing bacterial surface hydrophobicity, decreasing bacterial aggregation ability and inhibiting swarming motility Interestingly, the burdock leaf fraction inhibited the secretion of quorum sensing (QS) signaling molecule 3-oxo-C12-HSL and interfered quorum sensing...
November 12, 2016: Journal of Applied Microbiology
Seyed Karim Hassaninejad-Darzi, Mohammad Torkamanzadeh
One of the main difficulties in quantification of dyes in industrial wastewaters is the fact that dyes are usually in complex mixtures rather than being pure. Here we report the development of two rapid and powerful methods, partial least squares (PLS-1) and artificial neural network (ANN), for spectral resolution of a highly overlapping ternary dye system in the presence of interferences. To this end, Crystal Violet (CV), Malachite Green (MG) and Methylene Blue (MB) were selected as three model dyes whose UV-Vis absorption spectra highly overlap each other...
November 2016: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Sylvain Sergent, Masato Takiguchi, Hideaki Taniyama, Akihiko Shinya, Eiichi Kuramochi, Masaya Notomi
Nanowire-induced SiN photonic crystal (PhC) nanocavities specifically designed for the ultra-violet and visible range are investigated by three-dimensional finite-difference time-domain calculations. As opposed to their silicon PhC counterpart, we find that the formation of nanowire-induced two-dimensional (2D) SiN PhC nanocavities is more challenging because of the low refractive index of SiN. We thus discuss optimization strategies to circumvent such difficulties and we investigate the influence of critical design parameters such as PhC geometry, as well as nanowire geometry and position...
November 14, 2016: Optics Express
Zhen Li, Shouzhen Jiang, Yanyan Huo, Mei Liu, Cheng Yang, Chao Zhang, Xiaoyun Liu, Yingqing Sheng, Chonghui Li, Baoyuan Man
In this work, a facile and effective method for controlled-layer and large-area MoS<sub>2</sub> films encapsulated Au nanoparticle hybrids is developed. With accurate Ar plasma treatment time control, the large-area MoS<sub>2</sub> layers can be obtained from monolayer to trilayer. The fabricated MoS<sub>2</sub>@Au NPs with higher surface area exhibit excellent Raman enhanced effect for aromatic organic molecules (rhodamine 6G and crystal violet) and achieve the best when the monolayer MoS<sub>2</sub>@AuNPs was obtained...
November 14, 2016: Optics Express
Feng-Xia Liang, Deng-Yue Zhang, Jiu-Zhen Wang, Wei-Yu Kong, Zhi-Xiang Zhang, Yi Wang, Lin-Bao Luo
A highly sensitive ultraviolet A (UVA) and violet photodetector based on p-type single-layer graphene (SLG)-TiO<sub>2</sub> heterostructure was fabricated by transferring chemical vapor deposition derived SLG on the surface of commercial single-crystal TiO<sub>2</sub> wafer. Optoelectronic analysis reveals the as-fabricated Schottky junction PD was highly sensitive to light illumination in UVA and violet range, with peak sensitivity at 410 nm and excellent stability and reproducibility, but virtually blind to illumination with wavelength less than 350 nm or more than 460 nm...
November 14, 2016: Optics Express
Viraj C Gala, Nithya R John, Ashok M Bhagwat, Ajit G Datar, Prashant S Kharkar, Krutika B Desai
BACKGROUND & OBJECTIVES: The pathogenicity of the nosocomial pathogens, Pseudomonas aeruginosa and Acinetobacter baumannii is regulated by their quorum sensing (QS) systems. The objective of the present study was to examine the effect of the cold ethyl acetate extract of Tinospora cordifolia stem on virulence and biofilm development in the wild type and clinical strains of P. aeruginosa and A. baumannii. The study was further aimed to identify the probable active constituents in the plant extract...
July 2016: Indian Journal of Medical Research
M J Salas-Jara, E A Sanhueza, A Retamal-Díaz, C González, H Urrutia, A García
The ability of the human isolate Lactobacillus fermentum UCO-979C to form biofilm and synthesize exopolysaccharide on abiotic and biotic models is described. These properties were compared with the well-known Lactobacillus casei Shirota to better understand their anti-Helicobacter pylori probiotic activities. The two strains of lactobacilli synthesized exopolysaccharide as detected by the Dubois method and formed biofilm on abiotic and biotic surfaces visualized by crystal violet staining and scanning electron microscopy...
November 2016: Biofouling
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