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https://www.readbyqxmd.com/read/28447761/mir-21-inhibitor-suppresses-cell-proliferation-and-colony-formation-through-regulating-the-pten-akt-pathway-and-improves-paclitaxel-sensitivity-in-cervical-cancer-cells
#1
Guohui Du, Dongmei Cao, Lingzheng Meng
The present study aimed to investigate the role and the molecular mechanisms underlying the effects of microRNA-21 (miR-21) on the proliferation, apoptosis and colony formation of cervical cancer cells, and to examine the role of miR-21 in mediating the sensitivity of cervical cancer cells to paclitaxel (PTX). Reverse transcription‑quantitative polymerase chain reaction was employed to determine the level of miR‑21 in various cervical cancer and normal cervical cells. The results revealed that the expression levels of miR-21 in cervical cancer cells were markedly higher when compared with normal cervical cells...
March 16, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28441744/production-of-laccase-by-a-new-myrothecium-verrucaria-md-r-16-isolated-from-pigeon-pea-cajanus-cajan-l-millsp-and-its-application-on-dye-decolorization
#2
Jiao Sun, Na Guo, Li-Li Niu, Qing-Fang Wang, Yu-Ping Zang, Yuan-Gang Zu, Yu-Jie Fu
The present study was conducted to screen a laccase-producing fungal endophyte, optimize fermentation conditions, and evaluate the decolorization ability of the laccase. A new fungal endophyte capable of laccase-producing was firstly isolated from pigeon pea and identified as Myrothecium verrucaria based on a ITS-rRNA sequences analysis. Meanwhile, various fermentation parameters on the laccase production were optimized via response surface methodology (RSM). The optimal fermentation conditions were a fermentation time of five days, temperature 30 °C and pH 6...
April 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28440203/antimicrobial-biofilm-inhibitory-and-anti-infective-activity-of-metallic-nanoparticles-against-multi-drug-resistant-mrsa-and-pseudomonas-aeruginosa-pa01
#3
Jamuna Bai Ashwathanarayan, Ravishankar Rai Vittal
BACKGROUND: The emergence of drug resistant pathogens is a major concern to the scientific community. Novel approaches such as the use of functionalized nanomaterials with antimicrobial activity is required to treat infectious diseases. OBJECTIVE: In the present study, the metallic nanoparticles (iron, gold, zinc oxide and copper oxide) were evaluated for the antimicrobial, biofilm inhibitory and anti-infective activity against human pathogens methicillin resistant Staphylococcus aureus and Pseudomonas aeruginosa PA01...
April 24, 2017: Pharmaceutical Nanotechnology
https://www.readbyqxmd.com/read/28438935/candida-guilliermondii-complex-is-characterized-by-high-antifungal-resistance-but-low-mortality-a-report-on-22-cases-of-candidemia
#4
Laura Judith Marcos-Zambrano, Mireia Puig-Asensio, Felipe Pérez-García, Pilar Escribano, Carlos Sánchez-Carrillo, Oscar Zaragoza, Belén Padilla, Manuel Cuenca-Estrella, Benito Almirante, M Teresa Martin-Gómez, Patricia Muñoz, Emilio Bouza, Jesús Guinea
Objectives: The objectives of our study were to describe the characteristics of patients infected with C. guilliermondii candidemia and to perform an in-depth microbiological characterization of isolates and compare them with those of patients with C. albicans candidemia.Methods: We described the risk factors and outcome of 22 patients with candidemia caused by C. guilliermondii complex. Incident isolates were identified using molecular techniques, and susceptibility to fluconazole, anidulafungin and micafungin was studied...
April 24, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28436713/modified-dextran-heparin-based-triggered-release-microspheres-for-cardiovascular-delivery-of-therapeutic-drugs-using-protamine-as-a-stimulus
#5
Hoai X Nguyen, Edgar A O'Rear
In this study, we describe the synthesis of an amine-modified acetalated dextran polymer which is combined with heparin (HP) as the basis for a novel controlled release system. Dextran-amine (DEXAM) conjugates, synthesized using reductive amination, were incorporated into DEXAM/HP microspheres. HP binds to positively charged ammonium ions of the DEXAM conjugates, contributing to the structural integrity of the microspheres. Crystal violet (CV) was encapsulated inside DEXAM/HP microspheres as a model drug to test the system...
April 24, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28436680/extracting-optical-fiber-background-from-surface-enhanced-raman-spectroscopy-spectra-based-on-bi-objective-optimization-modeling
#6
Jie Huang, Tielin Shi, Zirong Tang, Wei Zhu, Guanglan Liao, Xiaoping Li, Bo Gong, Tengyuan Zhou
We propose a bi-objective optimization model for extracting optical fiber background from the measured surface-enhanced Raman spectroscopy (SERS) spectrum of the target sample in the application of fiber optic SERS. The model is built using curve fitting to resolve the SERS spectrum into several individual bands, and simultaneously matching some resolved bands with the measured background spectrum. The Pearson correlation coefficient is selected as the similarity index and its maximum value is pursued during the spectral matching process...
January 1, 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28434696/complete-eradication-of-biofilm-from-orthopedic-materials
#7
Jeffrey T Leary, Matthew M Werger, William H Broach, Lindsey N Shaw, Brandon G Santoni, Thomas L Bernasek, Steven T Lyons
BACKGROUND: Interest exists in finding alternatives to current management strategies in periprosthetic joint infections, which typically include a 2-stage revision with placement of an antibiotic spacer and delayed placement of a new implant. We studied the efficacy of autoclaving, ultrasonication, and mechanical scrubbing for sterilization and biofilm eradication on infected cobalt-chrome discs. METHODS: Strains of Staphylococcus aureus MRSA252 or Staphylococcus epidermidis RP62A were grown on the discs...
March 29, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28427622/efficient-removal-of-crystal-violet-and-methylene-blue-from-wastewater-by-ultrasound-nanoparticles-cu-mof-in-comparison-with-mechanosynthesis-method
#8
Amir Reza Abbasi, Maryam Karimi, Kim Daasbjerg
The present investigation reports the synthesis of CuBTC (BTC=1,3,5-benzenetricarboxylate) metal-organic frameworks (MOFs) under solid-state conditions and ultrasound irradiation. Herein, we study uptake and release properties of crystal violet (CV) and methylene blue (MB) from ultrasound nano-CuBTC MOF in comparison with mechanosynthesis method (bulk structure). The ultrasound-assisted methods give a decrease in the surface area as calculated from the reduced nitrogen adsorption capability. In comparison, the uptake of guest molecules on ultrasound nano-CuBTC is remarkable and clearly exceeds that of bulk structure in the aqueous solution of guests...
July 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28421635/a-homochiral-co%C3%AE-%C3%AE-16co%C3%AE-%C3%AE-%C3%AE-4-supertetrahedral-t4-cluster-from-a-racemic-ligand-with-ferromagnetic-behavior-and-high-photocatalytic-activity
#9
Yue-Ling Bai, Wei Du, Xiuping Yin, Jianhui Fang, Shourong Zhu, Jun Tao
A homochiral mixed-valence cobalt cluster [CoΙΙ16CoΙΙΙ4(μ6-O)4(μ3-OH)12(S-bme)12(OAc)6]Cl6·5CH3OH·18H2O (1, Hbme = 1H-(benzimidazol-2-yl)ethanol) was synthesized from a racemic ligand and three cobalt salts of CoCl2·6H2O, Co(Ac)2·4H2O and Co(NO3)2·6H2O in a DMF/MeOH mixed solvent. The enantioselective coordination occurs when a large excess of cobalt ions added in the solution and only the S-configuration of the racemic ligand involved in crystallization. The CD spectra of three crystal samples show identical Cotton signals, indicating the repeatability and the enantiomeric purity of the single crystals...
April 19, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28420830/antimicrobial-and-antibiofilm-activities-of-mta-supplemented-with-bismuth-lipophilic-nanoparticles
#10
Rene Hernandez-Delgadillo, Casiano Del Angel-Mosqueda, Juan Manuel Solís-Soto, Silvia Munguia-Moreno, Nayely Pineda-Aguilar, Rosa Isela Sánchez-Nájera, Shankararaman Chellam, Claudio Cabral-Romero
The objective of this work was to determine the antimicrobial and antibiofilm properties of mineral trioxide aggregate (MTA) supplemented with bismuth lipophilic nanoparticles (BisBAL NPs). The antimicrobial activity of the composite MTA-BisBAL NPs was determined by the disk diffusion assay, while antibiofilm activity was analyzed by fluorescence microscopy. The cytotoxicity of MTA-BisBAL NPs was determined on human gingival fibroblasts by optical microscopy and crystal violet staining. MTA-BisBAL NPs inhibited the growth of Enterococcus faecalis, Escherichia coli, and Candida albicans and also detached the biofilm of fluorescent E...
April 18, 2017: Dental Materials Journal
https://www.readbyqxmd.com/read/28417524/biofilms-of-candida-spp-from-the-ocular-conjunctiva-of-horses-with-reduced-azole-susceptibility-a-complicating-factor-for-the-treatment-of-keratomycosis
#11
Raimunda Sâmia Nogueira Brilhante, Paula Vago Bittencourt, Débora de Souza Collares Castelo-Branco, Glaucia Morgana de Melo Guedes, Jonathas Sales de Oliveira, Lucas Pereira Alencar, Rossana de Aguiar Cordeiro, Mariana Pinheiro, Evilázio Fernandes Nogueira-Filho, Waldemiro de Aquino Pereira-Neto, José Júlio Costa Sidrim, Marcos Fábio Gadelha Rocha
OBJECTIVES: This study aimed to assess the biofilm-forming ability of Candida spp. from the ocular conjunctiva of horses and to investigate the antifungal susceptibility of these biofilms. PROCEDURES: Initially, the biofilm-forming ability of 15 strains was assessed by crystal violet staining, which reveals the fungal biomass adhered to the polystyrene plates, and scanning electron microscopy. Then, the minimum inhibitory concentrations (MICs) of amphotericin B, fluconazole, itraconazole, and caspofungin were initially determined against strains in planktonic form...
April 18, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28417271/antimicrobial-effects-of-manuka-honey-on-in-vitro-biofilm-formation-by-clostridium-difficile
#12
M Piotrowski, P Karpiński, H Pituch, A van Belkum, P Obuch-Woszczatyński
Clostridium difficile is the cause of the nosocomial C. difficile infection (CDI). The conventional antibiotics used in CDI therapy are often unsuccessful, and recurrent infections may occur. Biofilm formation by C. difficile is associated with chronic or recurrent infections; biofilms may contribute to virulence and impaired antimicrobial efficacy. Manuka honey, derived from the Manuka tree (Leptospermum scoparium), is known to exhibit antimicrobial properties that are associated with its significant content of methylglyoxal, a natural antibiotic...
April 18, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28414038/modulation-of-staphylococcus-epidermidis-rp62a-extracellular-polymeric-layer-by-marine-cyclic-dipeptide-cyclo-l-leucyl-l-prolyl-thwarts-biofilm-formation
#13
Shanmugaraj Gowrishankar, Shunmugiah Karutha Pandian
Staphylococcus epidermidis is the most frequent cause of biofilm mediated implant-associated infections. Extracellular polymeric substance (EPS) is a key component of most biofilms and in pathogens it specifically protects the entrenched-bacterial cells from antibiotics and hosts immune response, and thereby makes the infection ineradicable. Recently, the prominence of cyclic dipeptides in interfering with biofilms and the associated virulence factors of pathogens has offered an alternative to eliminate difficult-to-treat infections...
April 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28410397/a-bis-sulphamoylated-estradiol-derivative-induces-ros-dependent-cell-cycle-abnormalities-and-subsequent-apoptosis
#14
Michelle Helen Visagie, Iman van den Bout, Anna Margaretha Joubert
Clinical trials have revealed that the potential anticancer agent, 2-methoxyestradiol (2ME2) has limitations due to its low bioavailability. Subsequently, 2ME2 derivatives including (8R,13S,14S,17S)-2-ethyl-13-methyl-7,8,9,11,12,13,14,15,16,17-decahydro-6H-cyclopenta[a]phenanthrane-3,17-diyl bis(sulphamate) (EMBS) have shown improved efficacies in inducing apoptosis. However, no conclusive data exist to explain the mode of action exerted by these drugs. This study investigated the mode of action used by EMBS as a representative of the sulphamoylated 2ME2 derivatives...
2017: PloS One
https://www.readbyqxmd.com/read/28408902/the-naturally-occurring-host-defense-peptide-ll-37-and-its-truncated-mimetics-ke-18-and-kr-12-have-selected-biocidal-and-antibiofilm-activities-against-candida-albicans-staphylococcus-aureus-and-escherichia-coli-in-vitro
#15
Yu Luo, Denise T F McLean, Gerard J Linden, Danny F McAuley, Ronan McMullan, Fionnuala T Lundy
Amongst the recognized classes of naturally occurring antimicrobials, human host defense peptides are an important group with an advantage (given their source) that they should be readily translatable to medicinal products. It is also plausible that truncated versions will display some of the biological activities of the parent peptide, with the benefit that they are less costly to synthesize using solid-phase chemistry. The host defense peptide, LL-37, and two truncated mimetics, KE-18 and KR-12, were tested for their inhibitory effects and antibiofilm properties against Candida albicans, Staphylococcus aureus, and Escherichia coli, microorganisms commonly implicated in biofilm-related infections such as ventilator-associated pneumonia (VAP)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28406026/multiple-color-generating-cu-in-ga-s-se-2-thin-film-solar-cells-via-dichroic-film-incorporation-for-power-generating-window-applications
#16
Gang Yeol Yoo, Jae-Seung Jeong, Soyoung Lee, Youngki Lee, Hee Chang Yoon, Van Ben Chu, Gi Soon Park, Yun Jeong Hwang, Woong Kim, Byoung Koun Min, Young Rag Do
There are four prerequisites when applying all types of thin-film solar cells to power-generating window photovoltaics (PVs): high power-generation efficiency, longevity and high durability, semitransparency or partial-light transmittance, and colorful and aesthetic value. Solid-type thin-film Cu(In,Ga)S2 (CIGS) or Cu(In,Ga)(S,Se)2 (CIGSSe) PVs nearly meet the first two criteria, making them promising candidates for power-generating window applications if they can transmit light to some degree and generate color with good aesthetic value...
April 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28405263/direct-contact-low-frequency-ultrasound-clearance-of-biofilm-from-metallic-implant-materials
#17
Mark S Granick, Chaitra Paribathan, Mayilvahanan Shanmugam, Narayanan Ramasubbu
Introduction: Biofilms are recognized as a significant deterrent to wound healing and to the management of exposed or infected surgical implants. Biofilms can be disrupted by a variety of enzymatic and mechanical interventions. This experiment was designed to determine whether direct-contact low-frequency ultrasound has the ability to clear biofilms and what then happens to the released bacteria. Methods:Staphylococcus epidermidis biofilm was grown on the surfaces of metallic discs composed of titanium and stainless steel, comparable with the alloys used in surgical implants...
2017: Eplasty
https://www.readbyqxmd.com/read/28402186/inhibition-of-hafnia-alvei-h4-biofilm-formation-by-the-food-additive-dihydrocoumarin
#18
Hong Man Hou, Feng Jiang, Gong Liang Zhang, Jia Y Wang, Ya H Zhu, Xin Y Liu
Quorum sensing (QS) is an intercellular signaling and gene regulatory mechanism that is implicated in food spoilage caused by bacteria. Thus, blocking QS may suppress QS-controlled phenotypes of these bacteria that are responsible for food spoilage. Biofilm formation is closely related to bacterial infection, and it is also a major mechanism responsible for the increased resistance of biofilm-associated bacteria to antimicrobial drugs. Food spoilage and biofilm formation caused by food-related bacteria have posed a significant problem for the food industry...
April 12, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28401747/dynamics-of-mono-and-dual-species-biofilm-formation-and-interactions-between-staphylococcus-aureus-and-gram-negative-bacteria
#19
Jitka Makovcova, Vladimir Babak, Pavel Kulich, Josef Masek, Michal Slany, Lenka Cincarova
Microorganisms are not commonly found in the planktonic state but predominantly form dual- and multispecies biofilms in almost all natural environments. Bacteria in multispecies biofilms cooperate, compete or have neutral interactions according to the involved species. Here, the development of mono- and dual-species biofilms formed by Staphylococcus aureus and other foodborne pathogens such as Salmonella enterica subsp. enterica serovar Enteritidis, potentially pathogenic Raoultella planticola and non-pathogenic Escherichia coli over the course of 24, 48 and 72 h was studied...
April 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28401535/nano-hydroxyapatite-blasted-titanium-surface-affects-pre-osteoblast-morphology-by-modulating-critical-intracellular-pathways
#20
Fábio Bezerra, Marcel R Ferreira, Giselle N Fontes, Célio da Costa Fernandes, Denise C Andia, Nilson C Cruz, Rodrigo A da Silva, Willian F Zambuzzi
Since intracellular signalling pathways are proposed to predict the quality of cell-surface relationship, this study addressed pre-osteoblast behaviour in response to nano hydroxyapatite (HA)-blasted titanium (Ti) surface by exploring critical intracellular pathways and pre-osteoblast morphological change. Physicochemical properties were evaluated by atomic force microscopy and wettability considering water contact angle of three differently texturized Ti surfaces: Machined (Mac), Dual acid-etching (DAE) and nano hydroxyapatite-blasted (nHA)...
April 12, 2017: Biotechnology and Bioengineering
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