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crystal violet

Maofeng Zhang, Xiyan Zhang, Liaowang Tong, Yaping Wang, Dexin Ou, Jumei Zhang, Qingping Wu, Yingwang Ye
Cronobacter malonaticus is one of the opportunistic food-borne pathogens in powdered infant formula and has unusual abilities to survive under environmental stresses such as osmotic conditions. However, the genes involved in osmotic stress have received little attention in C. malonaticus. Here, genes involved in osmotic stress were determined in C. malonaticus using a transposon mutagenesis approach. According to the growth of mutants (n = 215) under 5.0% NaCl concentration, the survival of 5 mutants under osmotic stress was significantly decreased compared with that of the wild type strain...
February 14, 2018: Journal of Dairy Science
Satoshi Otani, Kazufumi Hiramatsu, Kazuhiko Hashinaga, Kosaku Komiya, Kenji Umeki, Kenji Kishi, Jun-Ichi Kadota
Pseudomonas aeruginosa exhibits the biofilm mode of growth and causes chronic as well as acute infections in humans. Several reports have shown that the treatments with sub-minimum inhibitory concentrations (sub-MICs) of antimicrobial agents influence biofilm formation by P. aeruginosa. The antibiotic ceftazidime (CAZ) is used to treat P. aeruginosa infections, but few studies have examined the effects of β-lactams on biofilm formation by P. aeruginosa. In this study, we investigated the role of sub-MICs of CAZ in the formation of P...
February 12, 2018: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Manli Shen, Lu Fu, Jianhua Tang, Mingyu Liu, Youtao Song, Fangyuan Tian, Zhigang Zhao, Zhaohong Zhang, Dionysios D Dionysiou
In this study, novel spinel-NiFe 2 O 4 /natural mineral (sepiolite, diatomite and kaolinite) composites were developed using microwave (MW) hydrothermal method, and applied in MW-induced catalytic degradation (NiFe 2 O 4 /natural mineral/MW) of organic pollutants such as sodium dodecyl benzene sulfonate (SDBS), azo fuchsine (AF), methyl parathion (MP), and crystal violet (CVL) in solution. Catalytic activities of three NiFe 2 O 4 /natural mineral composites were compared. The effects of material synthesis process parameters such as molar ratios of NiFe 2 O 4 and natural mineral, and pH of precursor solutions for synthesizing catalysts, and degradation parameters such as MW irradiation time and catalyst reuse cycles were also investigated...
February 6, 2018: Journal of Hazardous Materials
S Benito, A Ferrer, S Benabou, A Aviñó, R Eritja, R Gargallo
Guanine-rich sequences may fold into highly ordered structures known as G-quadruplexes. Apart from the monomeric G-quadruplex, these sequences may form multimeric structures that are not usually considered when studying interaction with ligands. This work studies the interaction of a ligand, crystal violet, with three guanine-rich DNA sequences with the capacity to form multimeric structures. These sequences correspond to short stretches found near the promoter regions of c-kit and SMARCA4 genes. Instrumental techniques (circular dichroism, molecular fluorescence, size-exclusion chromatography and electrospray ionization mass spectrometry) and multivariate data analysis were used for this purpose...
February 6, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Rekha Yamini Kosuru, Md Aashique, Aisha Fathima, Amrita Roy, Soumen Bera
AIM: To understand the effects of gallic acid (GA) on ampicillin (Amp) sensitive or resistant strain of Pseudomonas sp. and also in modulating the corresponding biofilms. METHODOLOGY: The cell viability was determined by broth dilution, dry weight and CFU assays. Biofilm formation was measured by crystal violet assay while oxygen consumption rate was measured to verify the metabolic status of the cells. The membrane damage and drug efflux/accumulation were studied by fluorimetric assays...
February 15, 2018: Future Microbiology
Ju E Morozova, V V Syakaev, Ya V Shalaeva, A M Ermakova, I R Nizameev, M K Kadirov, E Kh Kazakova, A I Konovalov
The association of branched polyethyleneimine (PEI) with a series of octacarboxy-calixresorcinarenes bearing different low-rim substituents leads to the formation of nanosized supramolecular complexes. The PEI-macrocycle complexes have fine-tunable sizes regulated by variations in the self-association capacity of the calixresorcinarenes. In the supramolecular complexes, hydrophobic fragments of the polymer and calixresorcinarenes form cooperative hydrophobic domains which provide synergistic enhancement of guest molecule binding...
February 14, 2018: Soft Matter
Peter Muturi, Junping Yu, Junhua Li, Mengwei Jiang, Alice Nyambura Maina, Samuel Kariuki, Francis B Mwaura, Hongping Wei
AIMS: Isolation and characterisation of pectolytic bacteria associated with soft rot disease of potatoes in Nakuru, Kenya, to provide basis for the development of disease control measures. METHODS AND RESULTS: Potato tubers showing symptoms of soft rot were collected from different farms in Molo and Mau Narok regions within Nakuru county. Isolation was done using crystal violet pectate medium (CVPM). Out of 71 isolates that showed growth on crystal violet pectate medium, pathogenicity tests revealed that 36 of them had the ability to macerate tissues of potato tubers...
February 13, 2018: Journal of Applied Microbiology
Satish Kumar Rajasekharan, Jin-Hyung Lee, Yueju Zhao, Jintae Lee
Yeast-mold mycobiota inhabit several natural ecosystems, in which symbiotic relationships drive strategic pathoadaptation. Mycotoxins are metabolites produced by diverse mycotoxigenic fungi as a defense against yeasts, though at times yeasts secrete enzymes that degrade, detoxify, or bio-transform mycotoxins. The present study is focused on the in vitro inhibitory effects of zearalenone (ZEN), a F2 mycotoxin produced by several Fusarium and Gibberella species, on different microbial strains. ZEN exhibited no effect on the planktonic growth or biofilms of several Gram positive and negative bacteria at the tested concentrations...
March 2018: Indian Journal of Microbiology
Wei-Hsuan Hsu, Yu-Chun Lin, Bo-Rui Chen, She-Ching Wu, Bao-Hong Lee
Mitochondrial function is applied as oxidative stress and neuronal damage index. In this study, d-galactose was used to induce free radicals production and neuronal damage in HN-h cells, and the effect of novel 43 kDa protein isolated from oyster on anti-mitochondrial dysfunction and zinc-binding ability were evaluated. Crystal violet stain results indicated zinc-binding protein of oyster (ZPO) attenuated neuronal cell death induced by 100 mM of d-galactose on HN-h cells in a dose-dependent manner. ZPO alleviated mitochondrial inactivation, mitochondrial membrane potential decreasing, oxidative stress, and fusion/fission state in non-cytotoxic concentration of d-galactose (50 mM)-treated HN-h cells...
February 9, 2018: Food and Chemical Toxicology
Yosuke Toya, Masaki Endo, Risaburo Akasaka, Jun Urushikubo, Takahiro Gonai, Kensuke Asakura, Shunichi Yanai, Keisuke Kawasaki, Makoto Eizuka, Noriyuki Uesugi, Shotaro Nakamura, Tamotsu Sugai, Takayuki Matsumoto
BACKGROUND AND AIMS: We aimed to investigate an association between clinicopathological features, including immunohistochemical mucin phenotypes, and magnifying chromoendoscopic findings with crystal violet staining (ME-CV) in non-ampullary duodenal epithelial tumors (NADETs). METHODS: A total of 55 patients with NADET were divided into 3 groups by mucin phenotype: intestinal, gastrointestinal, or gastric. ME-CV findings were classified into 4 patterns: convoluted, leaf-like, reticular/sulciolar, and pinecone...
February 9, 2018: Digestion
Yvanna Carla S de Salgado, Marianna Boia Ferreira, Jessica Zablocki da Luz, Francisco Filipak Neto, Ciro Alberto de Oliveira Ribeiro
Murine melanoma cells B16F1 were exposed to the flame retardant and wood preservative chemical 2,4,6-tribromophenol (TBP) during 24 and 48 h, at the concentrations found in human diet. TBP-exposed cells had increased MTT and Alamar blue® metabolism and ABCB5 mRNA levels (qPCR), but the cells had decreased proliferation (crystal violet assay), migration (scratch assay), and drug-effux transporters activity (rhodamine B efflux assay). Exposure to TBP did not affect the cell viability (neutral red and annexin V-PI assays), colony formation (colony number, clonogenic assay), and the levels of reactive oxygen species (DCF probe) or P53 mRNA (qPCR)...
February 7, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Magdalena Wypij, Magdalena Świecimska, Joanna Czarnecka, Hanna Dahm, Mahendra Rai, Patrycja Golinska
AIMS: Presently, the effective antimicrobial agents have been limited by emergence of microbial strains with multi-drug resistance and biofilm formation potential. In the present study, we report remarkable antimicrobial activity of silver nanoparticles (AgNPs) synthesized from Streptomyces calidiresistens IF11 and IF17 strains, including inhibition of biofilm formation and synergistic effect of AgNPs and antibiotics against selected bacteria and yeasts. Cytotoxic effect of AgNPs on mammalian cell lines was also evaluated...
February 10, 2018: Journal of Applied Microbiology
Hatem A Mahmoud, Katabathini Narasimharao, Tarek T Ali, Kamal M S Khalil
TiO2 nanoparticles were synthesized from titanium isopropoxide by a simple peptization method using sulfuric, nitric, and acetic acids. The effect of peptizing acid on physicochemical and photocatalytic properties of TiO2 powders was studied. The structural properties of synthesized TiO2 powders were analyzed by using XRD, TEM, N2-physisorption, Raman, DR UV-vis, FTIR, and X-ray photoelectron spectroscopy techniques. The characterization results showed that acetic acid peptization facilitated the formation of pure anatase phase after thermal treatment at 500 °C; in contrast, nitric acid peptization led to a major rutile phase formation (67%)...
February 9, 2018: Nanoscale Research Letters
Géssica A Costa, Fernanda C P Rossatto, Aline W Medeiros, Ana Paula F Correa, Adriano Brandelli, Ana Paula G Frazzon, Amanda DE S DA Motta
The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocin-like substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods...
February 1, 2018: Anais da Academia Brasileira de Ciências
Emelia Hornam Adator, Meining Cheng, Rick Holley, Tim McAllister, Claudia Narvaez-Bravo
Biofilms are known to play important roles in bacterial survival and persistence in food-processing environments. This study aimed to determine the ability of the top 7 STEC serotypes to form biofilms on polystyrene (POL) and stainless steel (SS) plates and to quantify their survival and transfer from dry-surface biofilms to lettuce pieces. The ability of 14 STEC strains to form biofilms on these two materials at different exposure times and temperatures was assessed using crystal violet, Congo red and SEM...
January 25, 2018: International Journal of Food Microbiology
Wei Ren, Ruanhong Cai, Wanli Yan, Mingsheng Lyu, Yaowei Fang, Shujun Wang
This study evaluated the ability of a dextranase from a marine bacterium Catenovulum sp. (Cadex) to impede formation of Streptococcus mutans biofilms, a primary pathogen of dental caries, one of the most common human infectious diseases. Cadex was purified 29.6-fold and had a specific activity of 2309 U/mg protein and molecular weight of 75 kDa. Cadex showed maximum activity at pH 8.0 and 40 °C and was stable at temperatures under 30 °C and at pH ranging from 5.0 to 11.0. A metal ion and chemical dependency study showed that Mn2+ and Sr2+ exerted positive effects on Cadex, whereas Cu2+, Fe3+, Zn2+, Cd2+, Ni2+, and Co2+ functioned as inhibitors...
February 7, 2018: Marine Drugs
E Paluch, A Piecuch, E Obłąk, Ł Lamch, K A Wilk
The studies were aimed to contribute to the elucidation of the relationships between structure of the double-headed cationic surfactants - N,N-bis[3,3'-(dimethylamine)propyl]alkylamide dihydrochlorides and N,N-bis[3,3'-(trimethylammonio)propyl]alkylamide dibromides (alkyl: n-C9H19, n-C11H23, n-C13H27, n-C15H31), which are of particular interest, as they contain a labile amide group in the molecule and their antifungal activity. Therefore, the minimal inhibitory and fungicidal concentrations (MIC and MFC) of dicephalic surfactants against various fungi were tested using standardized methods...
January 31, 2018: Colloids and Surfaces. B, Biointerfaces
Do-Yeon Cho, Dong-Jin Lim, Calvin Mackey, Christopher G Weeks, Jaime A Peña Garcia, Daniel Skinner, Jessica W Grayson, Harrison S Hill, David K Alexander, Shaoyan Zhang, Bradford A Woodworth
BACKGROUND: Biofilms may contribute to refractory chronic rhinosinusitis (CRS), as they lead to antibiotic resistance and failure of effective clinical treatment. l-Methionine is an amino acid with reported biofilm-inhibiting properties. Ivacaftor is a cystic fibrosis transmembrane conductance regulator (CFTR) potentiator with mild antimicrobial activity via inhibition of bacterial DNA gyrase and topoisomerase IV. The objective of this study was to evaluate whether co-treatment with ivacaftor and l-methionine can reduce the formation of Pseudomonas aeruginosa biofilms...
February 7, 2018: International Forum of Allergy & Rhinology
Andrea Muras, Celia Mayer, Manuel Romero, Tamara Camino, Maria D Ferrer, Alex Mira, Ana Otero
Background: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent the biofilm formation by Streptococcus mutans, a pathogen involved in tooth decay. Objective: To obtain inhibition of biofilm formation by S. mutans by extracts obtained from the marine bacterium Tenacibaculum sp. 20J interfering with the AI-2 quorum sensing system. Design: The AI-2 inhibitory activity was tested with the biosensors Vibrio harveyi BB170 and JMH597. S. mutans ATCC25175 biofilm formation was monitored using impedance real-time measurements with the xCELLigence system®, confocal laser microscopy, and the crystal violet quantification method...
2018: Journal of Oral Microbiology
Patricia Muñoz, Caroline Agnelli, Jesús Guinea, Antonio Vena, Ana Alvarez-Uria, Laura Judith Marcos-Zambrano, Pilar Escribano, Maricela Valerio, Emilio Bouza
OBJECTIVES: The role of biofilm production in the outcome of candidemia remains under discussion. Current evidence relies on variable biofilm detection methods while evaluating distinct clinical endpoints. We aimed to determine the impact of biofilm production measured by metabolic activity (MA) and biomass (BM) on the prognosis of adults with candidemia. METHODS: Retrospective cohort including 280 adults with candidemia admitted from 2010 to 2016. BM was assessed using crystal violet binding stain and the XTT reduction assay was used to detect MA...
February 2, 2018: Clinical Microbiology and Infection
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