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https://www.readbyqxmd.com/read/29674848/biofilm-infections-between-scylla-and-charybdis-interplay-of-host-antimicrobial-peptides-and-antibiotics
#1
Sergey Chernysh, Natalia Gordya, Dmitry Tulin, Andrey Yakovlev
Purpose: The aim of this study is to improve the anti-biofilm activity of antibiotics. We hypothesized that the antimicrobial peptide (AMP) complex of the host's immune system can be used for this purpose and examined the assumption on model biofilms. Methods: FLIP7, the AMP complex of the blowfly Calliphora vicina containing a combination of defensins, cecropins, diptericins and proline-rich peptides was isolated from the hemolymph of bacteria-challenged maggots...
2018: Infection and Drug Resistance
https://www.readbyqxmd.com/read/29670843/bacteriophage-isolated-from-sewage-eliminates-and-prevents-the-establishment-of-escherichia-coli-biofilm
#2
Karla Veloso Gonçalves Ribeiro, Cleberson Ribeiro, Roberto Sousa Dias, Silvia Almeida Cardoso, Sergio Oliveira de Paula, Jose Cola Zanuncio, Leandro Licursi de Oliveira
Purpose: Biofilm growth exerts a negative impact on industry and health, necessitating the development of strategies to control. The objective of this work was study the lytic activity of the phage isolated from the sewage network in the formation and degradation of Escherichia coli biofilms. Methods: E. coli cultures were incubated in 96-well polystyrene microplates under controlled conditions to evaluate the biofilm formation. The E. coli cultures and established biofilms were treated with the suspensions of the vB_EcoM-UFV017 (EcoM017) bacteriophage obtained from sewage for 24 hours...
March 2018: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29666955/charge-ordering-in-the-metal-insulator-transition-of-v-doped-cro-2-in-the-rutile-structure
#3
Sarajit Biswas
Electronic, magnetic, and structural properties of pure and V-doped CrO2 were extensively investigated utilizing density functional theory. Usually, pure CrO2 is a half-metallic ferromagnet with conductive spin majority species and insulating spin minority species. This system remains in its half-metallic ferromagnetic phase even at 50% V-substitution for Cr within the crystal. The V-substituted compound Cr0.5 V0.5 O2 encounters metal-insulator transition upon the application of on-site Coulomb repulsion U = 7 eV preserving its ferromagnetism in the insulating phase...
April 17, 2018: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29666668/prospective-real-time-evaluation-of-diagnostic-performance-using-endocytoscopy-in-differentiating-neoplasia-from-non-neoplasia-for-colorectal-diminutive-polyps-%C3%A2-5-mm
#4
Takahiro Utsumi, Yasushi Sano, Mineo Iwatate, Hironori Sunakawa, Akira Teramoto, Daizen Hirata, Santa Hattori, Wataru Sano, Noriaki Hasuike, Kazuhito Ichikawa, Takahiro Fujimori
AIM: To clarify the diagnostic performance of endocytoscopy for differentiation between neoplastic and non-neoplastic colorectal diminutive polyps. METHODS: Patients who underwent endocytoscopy between October and December 2016 at Sano Hospital were prospectively recruited. When diminutive polyps (≤ 5 mm) were detected, the lesions were evaluated by endocytoscopy after being stained with 0.05% crystal violet and 1% methylene blue. The diminutive polyps were classified into five categories (EC 1a, 1b, 2, 3a, and 3b)...
April 15, 2018: World Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/29666543/spoilage-of-refrigerated-litopenaeus-vannamei-eavesdropping-on-acinetobacter-acyl-homoserine-lactones-promotes-the-spoilage-potential-of-shewanella-baltica
#5
Suqin Zhu, Haohao Wu, Caili Zhang, Jinxin Jie, Zunying Liu, Mingyong Zeng, Changyun Wang
Shewanella baltica and Acinetobacter are among the predominant spoilage bacteria in refrigerated shrimp ( Litopenaeus vannamei ). S. baltica are incapable of producing acyl-homoserine lactone (AHL) quorum sensing signals, but can respond to environmental AHLs. In this paper, Acinetobacter was found to produce three AHLs, i.e. N -butanoyl-l-homoserine lactone (C4-HSL), N -(3-oxohexanoyl)-l-homoserine lactone (O-C6-HSL) and N -(3-oxooctanoyl)-l-homoserine lactone (O-C8-HSL), according to thin-layer chromatography using the bioreporter Agrobacterium tumefaciens A136...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29665388/expression-and-characterization-of-novel-laccase-gene-from-pandoraea-sp-istkb-and-its-application
#6
Madan Kumar, Arti Mishra, Shashi Shekhar Singh, Shaili Srivastava, Indu Shekhar Thakur
In the present study, a non-blue laccase gene from previously reported lignin degrading bacterium, Pandoraea sp. ISTKB, was isolated, cloned and expressed in E. coli. Bioinformatics analysis of sequence discovered twin-arginine translocation signal sequence, copper binding motifs and presence of more random coil compare to helices and sheets in structure. The enzyme was found to be active on wide pH range and the pH optima was observed at pH 4 and 8 on substrate 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and 2,6-Dimethoxyphenol respectively...
April 14, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29651623/facile-synthesis-of-surface-functionalized-magnetic-nanocomposites-for-effectively-selective-adsorption-of-cationic-dyes
#7
Yani Hua, Juan Xiao, Qinqin Zhang, Chang Cui, Chuan Wang
A new magnetic nano-adsorbent, polycatechol modified Fe3 O4 magnetic nanoparticles (Fe3 O4 /PCC MNPs) were prepared by a facile chemical coprecipitation method using iron salts and catechol solution as precursors. Fe3 O4 /PCC MNPs owned negatively charged surface with oxygen-containing groups and showed a strong adsorption capacity and fast adsorption rates for the removal of cationic dyes in water. The adsorption capacity of methylene blue (MB), cationic turquoise blue GB (GB), malachite green (MG), crystal violet (CV) and cationic pink FG (FG) were 60...
April 12, 2018: Nanoscale Research Letters
https://www.readbyqxmd.com/read/29649653/a-label-free-cellulose-sers-biosensor-chip-with-improvement-of-nanoparticle-enhanced-lspr-effects-for-early-diagnosis-of-subarachnoid-hemorrhage-induced-complications
#8
Wansun Kim, Sung Ho Lee, Yong Jin Ahn, Seung Ho Lee, Jiwook Ryu, Seok Keun Choi, Samjin Choi
It is very difficult to predict some complications after subarachnoid hemorrhage (SAH), despite rapid advances in medical science. Herein, we introduce a label-free cellulose surface-enhanced Raman spectroscopy (SERS) biosensor chip with pH-functionalized, gold nanoparticle (AuNP)-enhanced localized surface plasmon resonance (LSPR) effects for identification of SAH-induced cerebral vasospasm and hydrocephalus caused by cerebrospinal fluid (CSF). The SERS biosensor chip was implemented by the synthesis reaction of the AuNPs, which were charged positively through pH level adjustment, onto a negatively-charged cellulose substrate with ξ = -30...
April 4, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29649626/novel-compounds-tad-1822-7-f2-and-f5-inhibited-hela-cells-growth-through-the-jak-stat-signaling-pathway
#9
Tianfeng Yang, Xianpeng Shi, Yuan Kang, Man Zhu, Mengying Fan, Dongdong Zhang, Yanmin Zhang
Cervical carcinoma remains the second most common malignancy with a high mortality rate among women worldwide. TAD-1822-7-F2 (F2) and TAD-1822-7-F5 (F5) are novel compounds synthesized on the chemical structure of taspine derivatives, and show an effective suppression for HeLa cells. Our study aims to confirm the potential targets of F2 and F5, and investigate the underlying mechanism of the inhibitory effect on HeLa cells. In this study, Real Time Cell Analysis and crystal violet staining assay were conducted to investigate the effect of F2 and F5 on HeLa cells proliferation...
April 9, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29648890/phelligridin-d-loaded-oral-nanotube-titanium-implant-enhances-osseointegration-and-prevents-osteolysis-in-rat-mandible
#10
Ji-Eun Kim, Jyoti Shrestha Takanche, Jeong-Seok Kim, Min-Ho Lee, Jae-Gyu Jeon, Il-Song Park, Ho-Keun Yi
Poor bone quality and osteolysis are the major causes of implant failure in dentistry. Here, this study tested the effect of phelligridin D-loaded nanotubes titanium (Ti) for bone formation around the dental implants. The purpose of this study was to enhance osseointegration of phelligridin D-loaded implant into the bone for bone formation and prevention of osteolysis. Cell viability, crystal violet staining, Western blot, alizarin red S staining, alkaline phosphatase activity, tartrate-resistant acid phosphatase staining, micro-computed tromography (μ-CT), hematoxylin and eosin (H&E) and immunohistochemical staining were used in vitro and in vivo to test the biocompatibility of phelligridin D...
April 12, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29630157/an-intensely-pruritic-pebbled-presentation
#11
Livia Francine Soriano, Conn McGrath, Mark Hawthorne, Arucha Linda Ekeowa-Anderson
Lichen amyloidosis (LA) is a form of primary localized cutaneous amyloidosis (PLCA) characterized by bilateral intensely itchy domed scaly hyperkeratotic papules. Lichen amyloidosis is rare and affects men more than women. It is uncommonly seen in the western world but more prevalent in Asia. These papules most typically affect the shins and occasionally the arms and torso. Lichen amyloidosis has been reported in association with autoimmune disorders and after prolonged exfoliation and friction of affected skin...
March 7, 2018: Dermatology Online Journal
https://www.readbyqxmd.com/read/29627449/rutin-inhibits-quorum-sensing-biofilm-formation-and-virulence-genes-in-avian-pathogenic-escherichia-coli
#12
Lu-Yuan Peng, Meng Yuan, Zhen-Qiang Cui, Zong-Mei Wu, Zhen-Jiang Yu, Ke Song, Bo Tang, Ben-Dong Fu
The study aimed to investigate whether rutin affects the quorum sensing (QS) of avian pathogenic Escherichia coli (APEC). In this study, APEC-O78 was selected as the test strain. We mainly examined the effects of rutin on the AI-2 secretion by bioluminescence assay, biofilm formation through a crystal violet staining method, and expression of virulence genes of APEC by qRT-PCR. We found that rutin can significantly interfering with QS through reducing the secretion of AI-2, inhibited the biofilm formation, and reduced the expression of virulence genes of APEC...
April 5, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29620347/electrically-triggered-small-molecule-release-from-poly-n-isopropylacrylamide-co-acrylic-acid-microgel-modified-electrodes
#13
Wenwen Xu, Yingnan Zhang, Yongfeng Gao, Michael J Serpe
A monolithic layer of poly( N-isopropylacrylamide- co-acrylic acid) microgels was deposited on an Au electrode and used for electrically triggered release of the small molecule crystal violet (CV), which was used as a model drug. CV was loaded into the surface-bound microgels by exposing them to a CV solution at pH 6.5, where the microgels are negatively charged and the CV is positively charged. The electrostatic attraction holds the CV inside of the microgels, while a decrease of the solution pH can neutralize the microgels and allow for CV release...
April 5, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29618248/surface-enhanced-raman-scattering-investigation-of-bovine-serum-albumin-by-au-nanoparticles-with-different-sizes
#14
Wei Xiaodan, Zheng Dawei, Zhang Ping, Lin Taifeng, Wang Huiqin, Zhu Yongwei
BACKGROUND: Surface-enhanced Raman scattering (SERS) has become a useful spectroscopic tool for studying biomolecule structures. The main types of plasmonic substrates used in biological systems are Au nanoparticles (AuNPs), whose surface plasmon resonance depends on the nanoparticle size, morphology, particle interspace, and so on. METHODS: In this study, AuNP colloids with different sizes were synthesized and used as the sensors to probe SERS signals of different biomarkers and biomolecules...
January 2018: Journal of Applied Biomaterials & Functional Materials
https://www.readbyqxmd.com/read/29614999/species-specific-characteristics-of-the-biofilm-generated-in-silicone-tube-an-in-vitro-study
#15
Dong Ju Kim, Joo-Hee Park, Minwook Chang
BACKGROUND: To investigate characteristics of biofilm which is usually found in silicone tube for nasolacrimal duct surgery and can be the root of chronic bacterial infections eventually resulted in surgical failure. METHODS: To form a biofilm, sterile silicone tube was placed in culture media of Staphylococcus aureus, Corynebacterium matruchotii, Pseudomonas aeruginosa, or Streptococcus pneumonia. Biofilms formed on these silicone tubes were fixed with 95% ethanol and stained with 0...
April 3, 2018: BMC Ophthalmology
https://www.readbyqxmd.com/read/29614315/a-novel-pharmacological-activity-of-caffeine-in-the-cholinergic-system
#16
Camila Fabiani, Ana Paula Murray, Jeremías Corradi, Silvia Susana Antollini
Cholinergic deficit is regarded as an important factor responsible for Alzheimer's disease (AD) symptoms. Acetylcholinesterase (AChE) and nicotinic receptor (AChR) are two molecular targets for the treatment of this disease. We found here that methanolic extracts of Camellia sinensis exhibited anticholinesterase activity and induced AChR conformational changes. From bioguided fractionation we confirmed that caffeine was the active compound exerting such effects. It is well-known that caffeine acts as an inhibitor of AChE and here we explored the effect of caffeine on the AChR by combining single channel recordings and fluorescent measurements...
March 31, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29604806/an-investigation-of-the-sites-occupied-by-atomic-barium-in-solid-xenon-a-2d-ee-luminescence-spectroscopy-and-molecular-dynamics-study
#17
Barry M Davis, Benoit Gervais, John G McCaffrey
A detailed characterisation of the luminescence recorded for the 6p1 P1 -6s1 S0 transition of atomic barium isolated in annealed solid xenon has been undertaken using two-dimensional excitation-emission (2D-EE) spectroscopy. In the excitation spectra extracted from the 2D-EE scans, two dominant thermally stable sites were identified, consisting of a classic, three-fold split Jahn-Teller band, labeled the blue site, and an unusual asymmetric 2 + 1 split band, the violet site. A much weaker band has also been identified, whose emission is strongly overlapped by the violet site...
March 28, 2018: Journal of Chemical Physics
https://www.readbyqxmd.com/read/29603682/adipose-derived-stem-cells-improve-erectile-function-partially-through-the-secretion-of-igf-1-bfgf-and-vegf-in-aged-rats
#18
J Yang, Y Zhang, G Zang, T Wang, Z Yu, S Wang, Z Tang, J Liu
Adipose-derived stem cells (ADSCs) have recently been considered as a promising therapy for erectile dysfunction (ED). However, the mechanism of ADSC-based therapy is unclear. Insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (VEGF) secreted by ADSCs were assessed in vitro. Sixteen 24-month-old male Sprague-Dawley rats were used for comparative analysis of 2-week treatment with labeled ADSCs or PBS. Eight additional 5-month-old rats were used as a young rat group...
March 30, 2018: Andrology
https://www.readbyqxmd.com/read/29600051/-in-vitro-effects-of-n-acetylcysteine-alone-and-combined-with-tigecycline-on-planktonic-cells-and-biofilms-of-acinetobacter-baumannii
#19
Jinlun Feng, Baomo Liu, Junwen Xu, Qinqin Wang, Lixia Huang, Weijun Ou, Jincui Gu, Jian Wu, Shaoli Li, Chao Zhuo, Yanbin Zhou
Background: Acinetobacter baumannii ( A. baumannii ), as a common opportunistic pathogen, has strong ability to form biofilms, which has led to drug resistance and chronic infections. The combination of N-acetylcysteine (NAC) and tigecycline (TGC) was demonstrated to synergistically inhibit biofilm-associated bacterial infections, including methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. The purpose of this study is to investigate the effect of NAC and TGC on planktonic cells and biofilms of A...
January 2018: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29595163/biosorption-of-cationic-dyes-by-par%C3%A3-chestnut-husk-bertholletia-excelsa
#20
Jordana Georgin, Bianca Silva Marques, Enrique Chaves Peres, Daniel Allasia, Guilherme Luiz Dotto
Pará chestnut husk (Bertholletia excelsa) (PCH), an agro-industrial waste largely generated in Brazil, was employed as a low-cost and efficient biosorbent to remove the cationic dyes Crystal Violet (CV) and Methylene Blue (MB) from aqueous media. PCH presented an amorphous structure containing carboxylic acids, esters, ketones and aldehydes on the surface. Non-porous and irregular particles were also observed. For both dyes, the biosorption capacity was favored under acid conditions. Equilibrium was attained within 40 min at 25 °C with a PCH dosage of 0...
March 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
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