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https://www.readbyqxmd.com/read/28823045/killing-activity-of-lfchimera-on-periodontopathic-bacteria-and-multispecies-oral-biofilm-formation-in-vitro
#1
Sopita Ruangcharoen, Waraporn Suwannarong, Marie Rossini Carmela T Lachica, Jan G M Bolscher, Kamran Nazmi, Watcharee Khunkitti, Suwimol Taweechaisupapong
Lactoferrin chimera (LFchimera), a heterodimeric peptide containing lactoferrampin (LFampin265-284) and a part of lactoferricin (LFcin17-30), possesses a broad spectrum of antimicrobial activity. However, there is no report on the inhibitory effects of LFchimera against multispecies oral biofilms. This study aimed to determine the effects of LFchimera in comparison to chlorhexidine digluconate (CHX) and minocycline hydrochloride (MH), on in vitro multispecies biofilms derived from subgingival plaque of periodontitis patients harboring Aggregatibacter actinomycetemcomitans...
August 19, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28822287/blue-light-induced-free-radicals-from-riboflavin-in-degradation-of-crystal-violet-by-microbial-viability-evaluation
#2
Ji-Yuan Liang, Jeu-Ming P Yuann, Zong-Jhe Hsie, Shiuh-Tsuen Huang, Chiing-Chang Chen
Crystal violet (CV) is applied in daily use mainly as a commercial dye and antimicrobial agent. Waste water containing CV may affect aquatic ecosystems. Riboflavin, also known as vitamin B2, is non-toxic and an essential vitamin required for the functions of the human body. Riboflavin is photosensitive to UV and visible light in terms of generating reactive oxygen species. This study investigated the potential application of blue light on riboflavin, so as to come up with an effective way of degrading CV during its treatment...
August 12, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28819697/mechanistic-understanding-of-crystal-violet-dye-sorption-by-woody-biochar-implications-for-wastewater-treatment
#3
Awanthi Wathukarage, Indika Herath, M C M Iqbal, Meththika Vithanage
Dye-based industries, particularly small and medium scale, discharge their effluents into waterways without treatment due to cost considerations. We investigated the use of biochars produced from the woody tree Gliricidia sepium at 300 °C (GBC300) and 500 °C (GBC500) in the laboratory and at 700 °C from a dendro bioenergy industry (GBC700), to evaluate their potential for sorption of crystal violet (CV) dye. Experiments were conducted to assess the effect of pH reaction time and CV loading on the adsorption process...
August 17, 2017: Environmental Geochemistry and Health
https://www.readbyqxmd.com/read/28804527/synthesis-and-characterisation-of-cross-linked-chitosan-composites-functionalised-with-silver-and-gold-nanoparticles-for-antimicrobial-applications
#4
Catherine Ryan, Emma Alcock, Finbarr Buttimer, Michael Schmidt, David Clarke, Martyn Pemble, Maria Bardosova
We present a study of a range of cross-linked chitosan composites with potential antimicrobial applications. They were formed by cross-linking chitosan and siloxane networks and by introducing silver and gold nanoparticles (NPs). The aim was to investigate whether adding the metal NPs to the chitosan-siloxane composite would lead to a material with enhanced antimicrobial ability as compared to chitosan itself. The composites were synthesised in hydrogel form with the metal NPs embedded in the cross-linked chitosan network...
2017: Science and Technology of Advanced Materials
https://www.readbyqxmd.com/read/28803003/pathogenic-features-and-characteristics-of-food-borne-pathogens-biofilm-biomass-viability-and-matrix
#5
REVIEW
Shiqi Lin, Ling Yang, Chen Gu, Bing Li, Dingqiang Chen, Lin Li, Zhenbo Xu
Biofilm is a ubiquitous growth pattern of bacterial species survival but is notorious for its threat on public health and food contamination. Extensive studies of the biofilm structure, formation, quantification, quorum sensing system and underlying control strategies have been reported during the past decades. Insightful elucidation of the pathogenic features and characteristic of bacterial biofilm can facilitate in devising appropriate control strategies for biofilm eradication. Therefore, this review mainly summarized the pathogenic features of biofilms from food borne microorganisms, including the biomass (which could be quantified using crystal violet and fluorogenic dye Syto9 assays), viability (which could be determined by tetrazolium salts, fluorescein diacetate, resazurin staining and alamar blue assays) and matrix (which are commonly detected by dimethyl methylene blue and wheat germ agglutinin assays)...
August 9, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28802722/quantification-of-biofilm-biomass-by-staining-non-toxic-safranin-can-replace-the-popular-crystal-violet
#6
Pernille Ommen, Natalia Zobek, Rikke Louise Meyer
Crystal violet staining is commonly used for quantification of biofilm formation, although it is highly toxic. Here we test safranin as a non-toxic replacement. Safranin staining provided similar results as crystal violet, but with higher reproducibility. We therefore recommend safranin staining for biofilm biomass quantification.
August 10, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28801810/effects-of-pbm-in-different-energy-densities-and-irradiance-on-maintaining-cell-viability-and-proliferation-of-pulp-fibroblasts-from-human-primary-teeth
#7
Nádia Carolina Teixeira Marques, Natalino Lourenço Neto, Mariel Tavares Oliveira Prado, Luciana Lourenço Ribeiro Vitor, Rodrigo Cardoso Oliveira, Vivien Thiemy Sakai, Carlos Ferreira Santos, Maria Aparecida Andrade Moreira Machado, Thais Marchini Oliveira
This study aimed to compare the effects of photobiomodulation (PBM) in different energy densities and irradiances on maintaining cell viability, and proliferation of pulp fibroblasts from human primary teeth (HPF) were cultured in DMEM and used between the fourth and eighth passages. Then, HPF were irradiated with the following different energy densities: 1.25 J/cm(2) (a), 2.50 J/cm(2) (b), 3.75 J/cm(2) (c), 5.00 J/cm(2) (d), and 6.25 J/cm(2) (e); but varying either the time of irradiation (groups 1a-1e) or the output power (groups 2a-2e)...
August 11, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28799927/heavy-metal-tolerance-and-removal-potential-in-mixed-species-biofilm
#8
Sandra Grujić, Sava Vasić, Ljiljana Čomić, Aleksandar Ostojić, Ivana Radojević
The aim of the study was to examine heavy metal tolerance (Cd(2+), Zn(2+), Ni(2+) and Cu(2+)) of single- and mixed-species biofilms (Rhodotorula mucilaginosa and Escherichia coli) and to determine metal removal efficiency (Cd(2+), Zn(2+), Ni(2+), Cu(2+), Pb(2+) and Hg(2+)). Metal tolerance was quantified by crystal violet assay and results were confirmed by fluorescence microscopy. Metal removal efficiency was determined by batch biosorption assay. The tolerance of the mixed-species biofilm was higher than the single-species biofilms...
August 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28799001/switch-on-fluorescent-strategy-based-on-crystal-violet-functionalized-cdte-quantum-dots-for-detecting-l-cysteine-and-glutathione-in-water-and-urine
#9
Zhen Sheng, Ligang Chen
The concentration of L-cysteine (Cys) and glutathione (GSH) is closely related to the critical risk of various diseases. In our study, a new rapid method for the determination of Cys and GSH in water and urine samples has been developed using a fluorescent probe technique, which was based on crystal violet (CV)-functionalized CdTe quantum dots (QDs). The original QDs emitted fluorescence light, which was turned off upon adding CV. This conjugation of CV and QDs could be attributed to electrostatic interaction between COO(-) of mercaptopropionic acid (MPA) on the surface of QDs and N(+) of CV in aqueous solution...
August 10, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28783550/characterization-of-the-clustered-regularly-interspaced-short-palindromic-repeats-sites-in-streptococcus-mutans-isolated-from-early-childhood-caries-patients
#10
Jing Chen, Tiancheng Li, Xuedong Zhou, Lei Cheng, Yuanyuan Huo, Jing Zou, Yuqing Li
OBJECTIVE: The aim of this study was to analyze the characteristics of the clustered regularly interspaced short palindromic repeats (CRISPR) sites in 45 clinical Streptococcus mutans strains and their relationship to the clinical manifestations of early childhood caries (ECC). METHODS: Forty-five S. mutans strains were isolated from the plaque samples taken from sixty-three children. CRISPR sites were sequenced and BLAST was used to compare these sites to those in the CRISPRTarget database...
July 29, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28774481/streptococcus-uberis-in-vitro-biofilm-production-in-response-to-carbohydrates-and-skim-milk
#11
Silvana A Dieser, Aluminé S Fessia, Miriam P Ferrari, Claudia G Raspanti, Liliana M Odierno
Streptococcus uberis has become one of the most important environmental pathogens associated with clinical and subclinical bovine mastitis. Biofilm confers to bacteria more resistance to physical and chemical agents as well as to different mechanisms of the innate immune system. The aim of this work was to evaluate the ability of in vitro biofilm production in 32 S. uberis isolates from bovine mastitis and identified by biochemical tests and subsequently confirmed by the amplification of the pauA gene. The isolates were cultivated in TMP broth and TMP broth with the addition of 0...
July 31, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28773010/synthesis-of-au-np-mos%C3%A2-quantum-dots-core-shell-nanocomposites-for-sers-bio-analysis-and-label-free-bio-imaging
#12
Xixi Fei, Zhiming Liu, Yuqing Hou, Yi Li, Guangcun Yang, Chengkang Su, Zhen Wang, Huiqing Zhong, Zhengfei Zhuang, Zhouyi Guo
In this work, we report a facile method using MoS₂ quantum dots (QDs) as reducers to directly react with HAuCl₄ for the synthesis of Au nanoparticle@MoS₂ quantum dots (Au NP@MoS₂ QDs) core@shell nanocomposites with an ultrathin shell of ca. 1 nm. The prepared Au NP@MoS₂ QDs reveal high surface enhanced Raman scattering (SERS) performance regarding sensitivity as well as the satisfactory SERS reproducibility and stability. The limit of detection of the hybrids for crystal violet can reach 0.5 nM with a reasonable linear response range from 0...
June 13, 2017: Materials
https://www.readbyqxmd.com/read/28771501/obliteration-of-bacterial-growth-and-biofilm-through-ros-generation-by-facilely-synthesized-green-silver-nanoparticles
#13
Shariq Qayyum, Mohammad Oves, Asad U Khan
Mangifera indica inflorescence aqueous extract was utilized for production of green AgNPs. Synthesized AgNPs were characterized by UV-vis spectrophotometry, XRD, TEM, FESEM and particles size analyzer. AgNPs showed minimum inhibitory concentrations (MICs) of 8 μg ml-1 and 16 μg ml-1 for Gram negative (K. pneumoniae, P. aeruginosa and E. coli) and Gram positive (S. mutans and S. aureus) strains, respectively which was relatively quite low compared to chemically synthesized silver nanoparticles. AgNPs inhibited 80% and 75% biofilms of E...
2017: PloS One
https://www.readbyqxmd.com/read/28762063/the-first-report-on-listeria-monocytogenes-producing-siderophores-and-responds-positively-to-n-acyl-homoserine-lactone-ahl-molecules-by-enhanced-biofilm-formation
#14
Milind Mohan Naik, Purva Bhangui, Chinmay Bhat
Listeria monocytogenes are Gram-positive well-known emerging food-borne pathogens causing listeriosis in humans. In the present study, we have isolated biofilm-forming Listeria sp. from utensils used by a local milk collection dairy society at Usgao Goa, which collects milk for Goa dairy. Through biochemical tests and 16S rRNA sequence analysis, the bacterium was confirmed to be L. monocytogenes and designated as strain BN3, having GenBank accession number MF095110. We report for the first time Gram-positive L...
July 31, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28759439/degradation-kinetics-of-organic-dyes-in-water-by-high-voltage-atmospheric-air-and-modified-air-cold-plasma
#15
S K Pankaj, Zifan Wan, William Colonna, Kevin M Keener
High voltage atmospheric cold plasma (HVACP) is a novel, non-thermal technology which has shown potential for degradation of various toxic components in wastewater. In this study, HVACP was used to examine the degradation kinetics of methyl red, crystal violet and fast green FCF dyes. HVACP discharge was found to be a source of reactive nitrogen and oxygen species. High voltage application completely degraded all dyes tested in less than 5 min treatment time. Plasma from modified gas (∼65% O2) further reduced the treatment time by 50% vs...
July 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28758725/superhydrophobic-and-white-light-activated-bactericidal-surface-through-a-simple-coating
#16
Gi Byoung Hwang, Adnan Patir, Elaine Allan, Sean P Nair, Ivan P Parkin
Bacterial adhesion and proliferation on surfaces are a challenge in medical and industrial fields. Here, a simple one-step technique is reported to fabricate self-cleaning and bactericidal surfaces. White, blue, and violet paints were produced using titanium dioxide nanoparticles, 1H,1H,2H,2H-perfluorooctyltriethoxysilane, crystal violet, toluidine Blue O, and ethanol solution. All of the painted surfaces showed superhydrophobicity in air, and even after hexadecane oil contamination, they retained water repellency and self-cleaning properties...
August 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28751708/using-persistent-photoconductivity-to-write-a-low-resistance-path-in-srtio3
#17
Violet M Poole, Slade J Jokela, Matthew D McCluskey
Materials with persistent photoconductivity (PPC) experience an increase in conductivity upon exposure to light that persists after the light is turned off. Although researchers have shown that this phenomenon could be exploited for novel memory storage devices, low temperatures (below 180 K) were required. In the present work, two-point resistance measurements were performed on annealed strontium titanate (SrTiO3, or STO) single crystals at room temperature. After illumination with sub-gap light, the resistance decreased by three orders of magnitude...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28748041/effect-of-azithromycin-on-a-red-complex-polymicrobial-biofilm
#18
Hwei Sze Ong, Orit Oettinger-Barak, Stuart G Dashper, Ivan B Darby, Kheng H Tan, Eric C Reynolds
Azithromycin has recently gained popularity for the treatment of periodontal disease, despite sparse literature supporting efficiency in treating periodontal bacterial biofilms. The aim of this study was to evaluate the effect of azithromycin on biofilms comprised of Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia in comparison to an amoxicillin and metronidazole combination. P. gingivalis W50, T. denticola ATCC35405, and T. forsythia ATCC43037 grown under anaerobic conditions at 37°C were aliquoted into 96-well flat-bottom plates in different combinations with addition of azithromycin or amoxicillin + metronidazole at various concentrations...
2017: Journal of Oral Microbiology
https://www.readbyqxmd.com/read/28744274/tobramycin-and-amikacin-delay-adhesion-and-microcolony-formation-in-pseudomonas-aeruginosa-cystic-fibrosis-isolates
#19
Elodie Olivares, Stéphanie Badel-Berchoux, Christian Provot, Benoît Jaulhac, Gilles Prévost, Thierry Bernardi, François Jehl
Cystic fibrosis (CF) patients are predisposed to chronic colonization of the major airways by Pseudomonas aeruginosa biofilms. Pulmonary infections, involving sessile bacteria, are the main cause of morbidity and mortality. As the eradication of antibiotic-resistant biofilms remains impossible, one key objective for the treatment of lung infections is to delay the switch of P. aeruginosa to a sessile phenotype. Few tools are currently available in hospital laboratories to evaluate the susceptibility of adherent microorganisms to antimicrobials...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28743086/effect-of-cinnamon-cinnamomum-verum-bark-essential-oil-on-the-halitosis-associated-bacterium-solobacterium-moorei-and-in-vitro-cytotoxicity
#20
Geneviève LeBel, Bruno Haas, Andrée-Ann Adam, Marie-Pier Veilleux, Amel Ben Lagha, Daniel Grenier
OBJECTIVES: Halitosis, also known as bad breath or oral malodour, is a condition affecting a large proportion of the population. Solobacterium moorei is a Gram-positive anaerobic bacterium that has been specifically associated with halitosis. In this study, we investigated the effects of essential oils, more particularly cinnamon bark oil, on growth, biofilm formation, eradication and killing, as well as hydrogen sulfide (H2S) production by S. moorei. METHODS: A broth microdilution assay was used to determine the antibacterial activity of essential oils...
July 12, 2017: Archives of Oral Biology
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