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https://www.readbyqxmd.com/read/28529588/downregulation-of-calbindin-1-a-calcium-binding-protein-reduces-the-proliferation-of-osteosarcoma-cells
#1
Zhengxiang Huang, Guojun Fan, Dongliang Wang
Osteosarcoma is the most common type of primary malignant bone tumor and has a high propensity to metastasize to the lungs and bones. Calbindin 1 (CALB1) is a constituent Ca2+ binding protein, which can prevent apoptotic death in several cell types induced through various pro-apoptotic signaling pathways. To investigate whether CALB1 is implicated in the tumor growth of human osteosarcoma, two different short hairpin RNAs (shRNAs) against CALB1 were used for CALB1-knockdown in osteosarcoma U2OS cells. The U2OS cells were divided into three groups: Two groups with CALB1 knockdown (CALB1-shRNA 1 and CALB1-shRNA 2) and one control group (Con-shRNA)...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28505288/effect-of-different-antibiotics-on-biofilm-produced-by-uropathogenic-escherichia-coli-isolated-from-children-with-urinary-tract-infection
#2
María José González, Luciana Robino, Victoria Iribarnegaray, Pablo Zunino, Paola Scavone
Recurrent urinary tract infections (UTI) occur frequently in children and women. Intracellular bacterial communities (IBC) and biofilm formation by Escherichia coli are risk factors for recurrence. The aim of the present study was to evaluate the effect of different antibiotics on biofilms by E. coli strains isolated from children with UTI and to correlate virulence factors and IBC with biofilm formation. 116 E. coli strains were tested for biofilm formation using the crystal violet microplate technique. 58...
May 12, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28502956/evaluation-of-biofilm-forming-ability-of-bacterial-strains-isolated-from-the-roof-of-an-old-house
#3
Marwa Jardak, Faten Abdelli, Rahma Laadhar, Raphael Lami, Didier Stien, Sami Aifa, Sami Mnif
The bacterial diversity associated with biofilm-forming ability was studied. Eighteen bacterial strains were isolated from a microbial film collected from the roof of an old house located in Sfax, Tunisia. The purity of these microorganisms was confirmed by microscopic observation after repeated streaking on a Tryptic Soy agar medium. Biofilm formation was estimated using preliminary tests including a motility test, microbial adhesion to solvents (MATS), and the Congo Red Agar method (CRA). Since these tests showed no significant result, microplate tests, such as crystal violet and resazurin assays, were used...
May 15, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28496447/inosine-released-from-dying-or-dead-cells-stimulates-cell-proliferation-via-adenosine-receptors
#4
Jin Chen, Ricardo A Chaurio, Christian Maueröder, Anja Derer, Manfred Rauh, Andriy Kost, Yi Liu, Xianming Mo, Axel Hueber, Rostyslav Bilyy, Martin Herrmann, Yi Zhao, Luis E Muñoz
INTRODUCTION: Many antitumor therapies induce apoptotic cell death in order to cause tumor regression. Paradoxically, apoptotic cells are also known to promote wound healing, cell proliferation, and tumor cell repopulation in multicellular organisms. We aimed to characterize the nature of the regenerative signals concentrated in the micromilieu of dead and dying cells. METHODS: Cultures of viable melanoma B16F10 cells, mouse fibroblasts, and primary human fibroblast-like synoviocytes (FLS) in the presence of dead and dying cells, their supernatants (SNs), or purified agonists and antagonists were used to evaluate the stimulation of proliferation...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28495625/nickel-nanoparticle-synthesis-using-camellia-sinensis-as-reducing-and-capping-agent-growth-mechanism-and-photo-catalytic-activity-evaluation
#5
Ismat Bibi, Shagufta Kamal, Adeel Ahmed, Munawar Iqbal, Shazia Nouren, Kashif Jilani, Nosheen Nazar, Muhammad Amir, Ansar Abbas, Sadia Ata, Farzana Majid
Recently, the biogenic synthesis of nanoparticle attracted the attention of scientific community due to its simplicity, ease and eco-friendly nature. In the present study, Camellia Sinensis (C. Sinensis) leaves extract was employed for the synthesis of nickel nanoparticles (NiNPs). The fabricated NiNPs were characterized by Scanning electron microscopy (SEM) and Energy-dispersive X-ray (EDX), magnetic properties and X-ray diffraction techniques. The photocatalytic activity (PCA) was evaluated by degrading crystal violet (CV) dye...
May 8, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28493889/characterization-of-a-novel-antibiofilm-effect-of-nitric-oxide-releasing-aspirin-ncx-4040-on-candida-albicans-isolates-from-denture-stomatitis-patients
#6
Francisco Madariaga-Venegas, Roberto Fernández-Soto, Luisa Fernanda Duarte, Nicole Suarez, Daniela Delgadillo, José A Jara, Ricardo Fernández-Ramires, Blanca Urzúa, Alfredo Molina-Berríos
Candida albicans biofilms play a key role in denture stomatitis, one of the most common oral pathologies in elderly people. Because biofilms are highly resistant to antifungals, new pharmacological strategies are needed. Aspirin and nitric oxide-donor molecules have both shown antibiofilm effects on C. albicans, making them promising candidates for treatment. In this study, we evaluated the antifungal/antibiofilm effect of a nitric-oxide releasing aspirin (NO-ASA) on C. albicans isolates from denture stomatitis patients in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28493679/light-induced-fluorescence-modulation-of-quantum-dot-crystal-violet-conjugates-stochastic-off-on-off-cycles-for-multicolor-patterning-and-super-resolution
#7
Sungwook Jung, Joonhyuck Park, Jiwon Bang, Jae-Yeol Kim, Cheolhee Kim, Yongmoon Jeon, Seung Hwan Lee, Ho Jin, Sukyung Choi, Bomi Kim, Woo Jin Lee, Chan-Gi Pack, Jong-Bong Lee, Nam Ki Lee, Sungjee Kim
Photoswitching or modulation of quantum dots (QDs) can be promising for many fields that include display, memory, and super-resolution imaging. However, such modulations have mostly relied on photomodulations of conjugated molecules in QD vicinity, which typically require high power of high energy photons at UV. We report a visible light-induced facile modulation route for QD-dye conjugates. QD crystal violets conjugates (QD-CVs) were prepared and the crystal violet (CV) molecules on QD quenched the fluorescence efficiently...
May 25, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28493317/attachment-and-biofilm-formation-by-selected-strains-of-salmonella-enterica-and-entrohemorrhagic-escherichia-coli-of-fresh-produce-origin
#8
Ran Han, Yaa Asantewaa Kafui Klu, Jinru Chen
This study compared the abilities of selected Salmonella enterica and enterohemorrhagic Escherichia coli (EHEC) strains of fresh produce origin to form biofilms on polystyrene surface and to attach to alfalfa and bean sprouts. Each of the 7 S. enterica and 4 EHEC inocula (2 mL; 10(7) CFU/mL) was placed in 6 different broths in 24-well polystyrene tissue culture plates at 28 °C for 1 to 7 d. Developed biofilms were quantified using the crystal violet binding assay. In a separate experiment, alfalfa and mung bean sprouts (5 g) were exposed to 25 mL inocula (10(7) CFU/mL) of S...
May 10, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28493157/determination-of-the-biofilm-production-capacities-and-characteristics-of-members-belonging-to-bacillaceae-family
#9
Arzu Coleri Cihan, Basar Karaca, Beste Piril Ozel, Tugba Kilic
The biofilm characteristics of many endospore-forming bacilli, especially the thermophiles are still unclear. In this study, a detailed identification and description of biofilm production characteristics of totally 145 isolates and reference strains belonging to Bacillaceae family, displaying thermophilic (n = 115), facultative thermophilic (n = 24) and mesophilic (n = 6) growth from genera Anoxybacillus, Geobacillus, Thermolongibacillus, Aeribacillus, Brevibacillus, Paenibacillus and Bacillus were presented...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28490150/a-simple-and-sensitive-vortex-assisted-ionic-liquid-dispersive-microextraction-and-spectrophotometric-determination-of-selenium-in-food-samples
#10
Esra Bağda, Mustafa Tüzen
In the present study, a novel and eco-friendly vortex-assisted ionic liquid-based microextraction method was developed for the determination of selenium in food. The microextraction method is based on the liberation of iodine in the presence of selenium; the liberated iodine reacts with I(-) to form I3(-). Anionic I3(-) reacts with cationic crystal violet dye, and the product is extracted into 1-hexyl-3-methylimidazolium hexafluorophosphate phase in the presence of Triton X-114. The proposed method is linear in the range of 2...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28479393/synthesis-and-swelling-characteristics-of-chitosan-and-cmc-grafted-sodium-acrylate-co-acrylamide-using-modified-nanoclay-and-examining-its-efficacy-for-removal-of-dyes
#11
M V Nagarpita, Pratik Roy, S B Shruthi, R R N Sailaja
Chitosan/carboxy methyl chitosan (CMC) grafted sodium acrylate-co-acrylamide/nanoclay superabsorbent nanocomposites have been synthesized in this study by following conventional and microwave assisted grafting methods. Microwave assisted grafting method showed higher grafting yield with enhanced reaction rate. Effect of nanoclay on water adsorption and swelling behaviour of both the composites in acidic, neutral and alkaline medium has been studied. Results showed enhanced swelling rate and water adsorption of both composites after adding 5% of silane treated nanoclay...
May 4, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28477054/neuroprotective-effects-of-sigesbeckia-pubescens-extract-on-glutamate-induced-oxidative-stress-in-ht22-cells-via-downregulation-of-mapk-caspase-3-pathways
#12
Md Rashedunnabi Akanda, Myung-Jin Kim, In-Shik Kim, Dongchoon Ahn, Hyun-Jin Tae, Md Mahfujur Rahman, Yang-Gyu Park, Jae-Won Seol, Hyeon-Hwa Nam, Byung-Kil Choo, Byung-Yong Park
Sigesbeckia pubescens (SP) is a traditional Chinese medicine, possessing antioxidant and anti-inflammatory activities. In this study, we evaluate the neuroprotective activities of SP extract on glutamate-induced oxidative stress in HT22 cells and the molecular mechanism underlying neuroprotection. We applied 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), crystal violet, reactive oxygen species (ROS), lactate dehydrogenase (LDH), quantitative real-time polymerase chain reaction (qPCR), and western blot analyses for assessing the neuroprotective effects of SP extract...
May 5, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28472126/growth-and-adherence-of-staphylococcus-aureus-were-enhanced-through-the-pge2-produced-by-the-activated-cox-2-pge2-pathway-of-infected-oral-epithelial-cells
#13
Yuxia Wang, Biao Ren, Xuedong Zhou, Shiyu Liu, Yujie Zhou, Bolei Li, Yaling Jiang, Mingyun Li, Mingye Feng, Lei Cheng
Staphylococcus aureus is a major pathogen of varieties of oral mucous infection. Prostaglandin E2 (PGE2) is a pro-inflammatory factor and Cyclooxygenase 2 (COX-2) is a critical enzyme of PGE2 biosynthesis. The purpose of this study is to investigate whether Staphylococcus aureus can increase PGE2 production of oral epithelial cells and how PGE2 functions in the growth and adherence of Staphylococcus aureus. mRNA levels of COX-2, fnbpA and fnbpB were estimated by quantitative PCR. PGE2 production was measured by Enzyme Linked Immunosorbent Assay (ELISA)...
2017: PloS One
https://www.readbyqxmd.com/read/28471120/evaluation-of-efficacy-of-1-crystal-violet-nuclear-fast-red-stain-compared-to-haematoxyline-eosin-stain-for-assessing-mitotic-figures-in-oral-premalignant-and-malignant-lesions
#14
Gauri Motiwale, Shradha Jaiswal, Ashok Vikey, Tejas Motiwale, Bhupesh Bagulkar, Atul Bhat, Prakhar Kapoor
Various chromosomal arrangements in cells undergoing division are referred to as Mitotic figure (MF). The abnormal excess of mitotic figures is commonly seen in oral epithelial dysplasia (ED) and oral squamous cell carcinoma (OSCC). In present study, we compared the number of mitotic figures in normal oral mucosa, epithelial dysplasia & OSCC sections with haematoxyline & eosine (H&E) and 1%Crystal Violet & Nuclear Fast Red (CV&NFR) stain, also the efficacy of the CV&NFR stain as compared to H & E stain. We investigated the correlation between the number of mitotic figures & grades of OSCC...
July 2016: Journal of Experimental Therapeutics & Oncology
https://www.readbyqxmd.com/read/28471113/antibacterial-mechanism-of-high-mobility-group-nucleosomal-binding-domain-2-on-the-gram-negative-bacteria-escherichia-coli
#15
Heng Li, Xiao-Fei Shen, Xin-E Zhou, Yan-E Shi, Lu-Xia Deng, Yi Ma, Xiao-Ying Wang, Jing-Yu Li, Ning Huang
OBJECTIVE: To investigate the antibacterial mechanism of high-mobility group nucleosomal-binding domain 2 (HMGN2) on Escherichia coli K12, focusing on the antibacterial and antibiofilm formation effects. Its chemotactic activity on human neutrophils was also investigated. METHODS: Human tissue-derived HMGN2 (tHMGN2) was extracted from fresh uterus fiber cystadenoma and purified by HP1100 reversed-phase high-performance liquid chromatography (RP-HPLC). Recombinant human HMGN2 (rHMGN2) was generated in E...
May 2017: Journal of Zhejiang University. Science. B
https://www.readbyqxmd.com/read/28470375/subtractive-protein-profiling-of-salmonella-typhimurium-biofilm-treated-with-dmso
#16
Mohd Fakharul Zaman Raja Yahya, Zazali Alias, Saiful Anuar Karsani
Salmonella typhimurium is an important biofilm-forming bacteria. It is known to be resistant to a wide range of antimicrobials. The present study was carried out to evaluate the effects of dimethyl sulfoxide (DMSO) against S. typhimurium biofilm and investigate whole-cell protein expression by biofilm cells following treatment with DMSO. Antibiofilm activities were assessed using pellicle assay, crystal violet assay, colony-forming unit counting and extracellular polymeric substance (EPS) matrix assay whilst differential protein expression was investigated using a combination of one dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, tandem mass spectrometry and bioinformatics...
May 4, 2017: Protein Journal
https://www.readbyqxmd.com/read/28468237/hypoxia-mediates-differential-response-to-anti-egfr-therapy-in-hnscc-cells
#17
Emilia Wiechec, Katharina Tiefenböck Hansson, Lisa Alexandersson, Jan-Ingvar Jönsson, Karin Roberg
Despite advances in the head and neck squamous cell carcinoma (HNSCC) treatment modalities, drug resistance and cancer recurrence are often reported. Hypoxia signaling through hypoxia-inducible factor 1 (HIF-1) promotes angiogenesis and metastasis by inducing epithelial-mesenchymal-transition (EMT). The aim of this study was to evaluate the impact of hypoxia on response to therapy as well as EMT and expression of stem cell markers in HNSCC cells. Five HNSCC cell lines (UT-SCC-2, UT-SCC-14, LK0412, LK0827, and LK0923) were selected for this study...
April 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28464918/growth-suppressive-effect-of-pegylated-arginase-in-malignant-pleural-mesothelioma-xenografts
#18
Sze-Kwan Lam, Yuan-Yuan Li, Shi Xu, Leanne Lee Leung, Kin-Pong U, Yan-Fang Zheng, Paul Ning-Man Cheng, James Chung-Man Ho
BACKGROUND: Malignant pleural mesothelioma (MPM) is a difficult-to-treat global disease. Pegylated arginase (BCT-100) has recently shown anti-tumor effects in hepatocellular carcinoma, acute myeloid leukemia and melanoma. This study aims to investigate the effects of PEG-BCT-100 in MPM. METHODS: A panel of 5 mesothelioma cell lines (H28, 211H, H226, H2052 and H2452) was used to study the in vitro effects of BCT-100 by crystal violet staining. The in vivo effects of BCT-100 were studied using 211H and H226 nude mice xenografts...
May 2, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28463662/antibiotic-susceptibility-of-planktonic-and-biofilm-grown-staphylococci-isolated-from-implant-associated-infections-should-mbec-and-nature-of-biofilm-formation-replace-mic
#19
Aaron J Brady, Garry Laverty, Deirdre F Gilpin, Patricia Kearney, Michael Tunney
PURPOSE: The purpose of this study was to develop an alternative, more clinically relevant approach to susceptibility reporting for implant-associated infections. Using 20 staphylococcal isolates, isolated from clinical implant infections, the majority (85 %) demonstrated biofilm-forming capabilities. A significantly increased minimum biofilm eradication concentration (MBEC) compared to minimum inhibitory concentration (MIC) breakpoint was obtained, with MBEC values greater than 256 µg ml-1 for the majority of bacteria...
April 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28463491/effective-passivation-and-tunneling-hybrid-a-siox-in-layer-in-ito-n-si-heterojunction-photovoltaic-device
#20
Ming Gao, Yazhou Wan, Yong Li, Baichao Han, Wenlei Song, Fei Xu, Lei Zhao, Zhongquan Ma
In this article, using controllable magnetron sputtering of indium tin oxide (ITO) materials on single crystal silicon at 100 °C, the optoelectronic heterojunction frame of ITO/a-SiOx(In)/n-Si is simply fabricated for the purpose of realizing passivation contact and hole tunneling. It is found that the gradation profile of indium (In) element together with silicon oxide (SiOx/In) within the ultrathin boundary zone between ITO and n-Si occurs and is characterized by X-ray photoelectron spectroscopy with the ion milling technique...
May 11, 2017: ACS Applied Materials & Interfaces
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