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https://www.readbyqxmd.com/read/28335303/detection-of-prohibited-fish-drugs-using-silver-nanowires-as-substrate-for-surface-enhanced-raman-scattering
#1
Jia Song, Yiqun Huang, Yuxia Fan, Zhihui Zhao, Wansong Yu, Barbara A Rasco, Keqiang Lai
Surface-enhanced Raman scattering or surface-enhanced Raman spectroscopy (SERS) is a promising detection technology, and has captured increasing attention. Silver nanowires were synthesized using a rapid polyol method and optimized through adjustment of the molar ratio of poly(vinyl pyrrolidone) and silver nitrate in a glycerol system. Ultraviolet-visible spectrometry, X-ray diffraction, and transmission electron microscopy were used to characterize the silver nanowires. The optimal silver nanowires were used as a SERS substrate to detect prohibited fish drugs, including malachite green, crystal violet, furazolidone, and chloramphenicol...
September 21, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28333393/laser-generated-shockwaves-enhance-antibacterial-activity-against-biofilms-in-vitro
#2
William Yao, Edward C Kuan, Nathan C Francis, Maie A St John, Warren S Grundfest, Zachary D Taylor
BACKGROUND AND OBJECTIVES: Bacterial biofilm formation within chronic wound beds, which provides an effective barrier against antibiotics, is a known cause of recalcitrant infections and a significant healthcare burden, often requiring repeated surgical debridements. Laser-generated shockwaves (LGS) is a novel, minimally invasive, and nonthermal modality for biofilm mechanical debridement which utilizes compressive stress waves, generated by photonic absorption in thin titanium films to mechanically disrupt the biofilm...
March 23, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28322109/tio2-nanorod-arrays-as-a-photocatalytic-coating-enhanced-antifungal-and-antibacterial-efficiency-of-ti-substrates
#3
Lu Jia, Jichuan Qiu, Lingqian Du, Zhi Li, Hong Liu, Shaohua Ge
AIM: To investigate the photocatalytic inactivation of fungi and bacteria mediated by TiO2 nanorod arrays (TNRs). MATERIALS & METHODS: The features of TNRs were characterized by scanning electron microscopy, atomic force microscopy, transmission electron microscopy, x-ray diffraction (XRD) and contact angle measurement. The antimicrobial efficiency was detected on biofilm and planktonic forms of Candida albicans, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis by crystal violet and XTT (2,3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide) assay, confocal laser scanning microscope and colony-forming assay...
March 21, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28303128/a-high-throughput-screening-platform-of-microbial-natural-products-for-the-discovery-of-molecules-with-antibiofilm-properties-against-salmonella
#4
Sonia Paytubi, Mercedes de La Cruz, Jose R Tormo, Jesús Martín, Ignacio González, Victor González-Menendez, Olga Genilloud, Fernando Reyes, Francisca Vicente, Cristina Madrid, Carlos Balsalobre
In this report, we describe a High-Throughput Screening (HTS) to identify compounds that inhibit biofilm formation or cause the disintegration of an already formed biofilm using the Salmonella Enteritidis 3934 strain. Initially, we developed a new methodology for growing Salmonella biofilms suitable for HTS platforms. The biomass associated with biofilm at the solid-liquid interface was quantified by staining both with resazurin and crystal violet, to detect living cells and total biofilm mass, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28301130/graphene-embedded-hydrogel-nanofibers-for-detection-and-removal-of-aqueous-phase-dyes
#5
Kyungun Im, Duong Nguyen Nguyen, Saerona Kim, Hye Jeong Kong, Yukyung Kim, Chul Soon Park, Oh Seok Kwon, Hyeonseok Yoon
A facile route to graphene/polymer hydrogel nanofibers was developed. An aqueous dispersion of graphene (containing >40% bilayer graphene flakes) stabilized by a functionalized water-soluble polymer with phenyl side chains was successfully electrospun to yield nanofibers. Subsequent vapor-phase cross-linking of the nanofibers produced graphene-embedded hydrogel nanofibers (GHNFs). Interestingly, the GHNFs showed chemical sensitivity to the cationic dyes methylene blue (MB) and crystal violet (CV) in the aqueous phase...
March 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28290651/adsorption-driven-catalytic-and-photocatalytic-activity-of-phase-tuned-in2s3-nanocrystals-synthesized-via-ionic-liquids
#6
Rahul Kumar Sharma, Yogendra Nath Chouryal, Sushmita Chaudhari, Jeganathan Saravanakumar, Suhash Ranjan Dey, Pushpal Ghosh
Phase tuned quantum confined In2S3 nanocrystals are accessible solvothermally using task-specific ionic liquids (ILs) as structure directing agents. Selective tuning of size, shape, morphology and, most importantly, crystal phase of In2S3 is achieved by changing the alkyl side chain length, the H-bonding and aromatic -stacking ability of the 1-alkyl-3- methylimidazolium bromide ILs, [Cnmim]Br (n=2,4,6,8 and 10). It is observed that crystallite size is significantly less when ILs are used compared to the synthesis without ILs keeping the other reaction parameters same...
March 14, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28288290/effect-of-commonly-prescribed-liquid-medications-on-streptococcus-mutans-biofilm-an-in-vitro-study
#7
Samantha A Clark, LaQuia A Vinson, George Eckert, Richard L Gregory
OBJECTIVE: This study addressed the effect of pediatric liquid antibiotic medications on Streptococcus mutans UA159. These suspensions commonly contain sugars such as sucrose to make them more palatable for children. The study was designed to evaluate the effects of oral liquid antibiotics on Streptococcus mutans growth and biofilm formation. STUDY DESIGN: A 24 hour culture of S. mutans was treated with various concentrations of liquid medications commonly prescribed to children for odontogenic or fungal infections- amoxicillin, penicillin VK, clindamycin, and nystatin...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28286870/d-amino-acids-do-not-inhibit-pseudomonas-aeruginosa-biofilm-formation
#8
Wee Tin K Kao, Mitchell Frye, Patricia Gagnon, Joseph P Vogel, Richard Chole
OBJECTIVE: Pseudomonas aeruginosa, a known biofilm-forming organism, is an opportunistic pathogen that plays an important role in chronic otitis media, tracheitis, cholesteatoma, chronic wounds, and implant infections. Eradication of biofilm infections has been a challenge because the biofilm phenotype provides bacteria with a protective environment from the immune system and antibiotics; thus, there has been great interest in adjunctive molecules that may inhibit biofilm formation or cause biofilm dispersal...
February 2017: Laryngoscope Investigative Otolaryngology
https://www.readbyqxmd.com/read/28281322/chemical-composition-antibacterial-antibiofilm-and-synergistic-properties-of-essential-oils-from-eucalyptus-globulus-labill-and-seven-mediterranean-aromatic-plants
#9
Maria Vieira, Lucinda J Bessa, M Rosário Martins, Sílvia Arantes, António P S Teixeira, Ângelo Mendes, Paulo Martins da Costa, Anabela D F Belo
Essential oils (EOs) from Eucalyptus globulus Labill. ssp. globulus and from Mediterranean autochthonous aromatic plants - Thymus mastichina L., Mentha pulegium L., Rosmarinus officinalis L., Calamintha nepeta (L.) Savi ssp. nepeta, Cistus ladanifer L., Foeniculum vulgare L., Dittrichia viscosa (L.) Greuter ssp. viscosa - were extracted by hydrodistillation and characterized by GC-FID and NMR spectroscopy. EOs were evaluated for antimicrobial properties against several bacterial strains, using diverse methods, namely, the agar disc-diffusion method, the microdilution method, the crystal violet assay and the Live/Dead staining for assessment of biofilm formation...
March 9, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28280998/virulence-factors-of-fluconazole-susceptible-and-fluconazole-resistant-candida-albicans-after-antimicrobial-photodynamic-therapy
#10
Fernanda Alves, Ewerton Garcia de Oliveira Mima, Renata Caroline Polato Passador, Vanderlei Salvador Bagnato, Janaína Habib Jorge, Ana Cláudia Pavarina
This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) mediated by Photodithazine® (PDZ) and LED light on the virulence factors of fluconazole-susceptible (CaS) and fluconazole-resistant (CaR) Candida albicans. Standardized suspensions of strains were prepared (10(7)), and after 48 h of biofilm formation, these strains were incubated with PDZ (100 mg/L) for 20 min and exposed to LED light (660 nm, 37.5 J/cm(2)). Additional samples were treated with PDZ or light only, and the control consisted of biofilms that received no treatment...
March 9, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28272044/physicochemical-effect-of-activation-temperature-on-the-sorption-properties-of-pine-shell-activated-carbon
#11
Agha Arslan Wasim, Muhammad Nasiruddin Khan
Activated carbons produced from a variety of raw materials are normally selective towards a narrow range of pollutants present in wastewater. This study focuses on shifting the selectivity of activated carbon from inorganic to organic pollutants using activation temperature as a variable. The material produced from carbonization of pine shells substrate was activated at 250°C and 850°C. Both adsorbents were compared with commercial activated carbon for the sorption of lead, cadmium, methylene blue, methyl blue, xylenol orange, and crystal violet...
March 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28267427/prevention-of-biomaterial-infection-by-pre-operative-incubation-with-human-cells
#12
Ramón Pérez-Tanoira, Antti A Aarnisalo, Kari K Eklund, Xia Han, Antti Soininen, Veli-Matti Tiainen, Jaime Esteban, Teemu J Kinnari
BACKGROUND: Cells of tissues and biofilm forming bacteria compete for the living space on the surface of an implant. We hypothesized the incubation of the implant (titanium, polydimethylsiloxane, and polystyrene surface) with human cells before implantation as a strategy to prevent bacterial adhesion and biofilm formation. METHODS: After 24 hours of incubation with human osteogenic sarcoma SaOS-2 cells (1 × 10(5) cells/mL), the materials were incubated for 4...
March 7, 2017: Surgical Infections
https://www.readbyqxmd.com/read/28266278/synthesis-and-preliminary-cytotoxicity-studies-of-1-1-4-5-dihydrooxazol-2-yl-1h-indazol-3-yl-3-phenylurea-and-3-phenylthiourea-derivatives
#13
Monika Wojciechowska, Łukasz Sitek, Aneta Sakowicz, Ewa Romejko, Piotr Szumlas, Martyna Korcz, Patrick J Bednarski, Franciszek Sączewski, Anita Kornicka
BACKGROUND: N-substituted 3-amino-1H-indazoles represent an interesting class of biologically active compounds. Among them, derivatives containing phenylurea moiety are of particular interest. Such compounds have been found to possess inhibitory activity against cancer cell growth. Additionally, various oxazoline-containing compounds have also been designed as potential anticancer agents. OBJECTIVE: The aim of this work was to obtain a new class of N-substituted 3-amino-1H-indazole derivatives with cytotoxic activity towards cancer cells...
March 6, 2017: Medicinal Chemistry
https://www.readbyqxmd.com/read/28266094/controlled-growth-of-gold-nanostars-effect-of-spike-length-on-sers-signal-enhancement
#14
Ganesh Venkatachalam, S V Sheen Mers, Shivakumar Umadevi
In this work, two different types of gold nanostars (Au NS) namely short-spiked nanostars (SSNS) and long-spiked nanostars (LSNS) are prepared by using hexagonal lyotropic liquid crystalline (LLC) phase as a template. Basically the formation, size and length of spikes or arms of the resultant Au NS are controlled by preparation either in a hexagonal LLC phase or in an isotropic phase. These NS are anchored onto ITO electrodes through self-assembled monolayer (SAM) of 3-mercaptopropyltrimethoxysilane (MPTMS) acting as a linker molecule...
March 7, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28250349/adsorption-capability-of-cationic-dyes-methylene-blue-and-crystal-violet-onto-poly-%C3%AE-glutamic-acid
#15
Fumihiko Ogata, Noriaki Nagai, Naohito Kawasaki
In this study, the adsorption capability of cationic dyes, which were methylene blue and crystal violet, by poly-γ-glutamic acid (PGA) in a single or binary solution system was investigated. The effect of the molecular weight of PGA, initial dye concentration, solution pH, and temperature on the adsorption of dyes was evaluated. The adsorption mechanism of dyes onto PGA was the interaction between -COOH group on the PGA surface and the polarity groups of dyes. These results indicated that PGA is useful for removal of dyes and cationic organic compounds from a single or binary solution system...
2017: Chemical & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28249235/rapid-fabrication-of-silver-nanoparticle-coated-filter-paper-as-sers-substrate-for-low-abundance-molecules-detection
#16
Wenxian Wei, Qingli Huang
Silver nanoparticles (Ag NPs) were fabricated on the fibers of the filter paper by the reaction between silver nitrate (AgNO3) and hydrazine hydrate (N2H4·H2O). By using the Ag NPs-coated paper, the limit of detection as low as 10(-11)M for Rhodamine B (RhB) and 10(-10)M for crystal violet (CV) was achieved. Moreover, the uniformity, reproducibility and stability of the Ag NPs-coated paper were also involved. Meanwhile, the detection of 10(-4)-10(-6)M moxifloxacin in deionized water and tap water was also carried out successfully by using the paper-based substrates...
February 24, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28244237/usefulness-of-confocal-laser-endomicroscopy-to-diagnose-ulcerative-colitis-associated-neoplasia
#17
Naoki Ohmiya, Noriyuki Horiguchi, Tomomitsu Tahara, Dai Yoshida, Hyuga Yamada, Mitsuo Nagasaka, Yoshihito Nakagawa, Tomoyuki Shibata, Tetsuya Tsukamoto, Makoto Kuroda
Chromoendoscopy, narrow-band imaging (NBI), and confocal laser endomicroscopy (CLE) have been introduced in ulcerative colitis (UC)-associated neoplasia surveillance. We aimed to determine the ability of CLE to differentiate among UC-associated neoplasia (differentiated type or undifferentiated type), sporadic adenoma, and circumscribed regenerative lesions. Of 665 patients with UC, we performed probe-based CLE (pCLE) on 12 patients with suspected UC-associated neoplasia in addition to magnifying chromoendoscopy with crystal violet and NBI...
February 28, 2017: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
https://www.readbyqxmd.com/read/28243912/formation-of-biofilms-by-foodborne-pathogens-and-development-of-laboratory-in-vitro-model-for-the-study-of-campylobacter-genus-bacteria-based-on-these-biofilms
#18
N R Efimochkina, I B Bykova, Yu M Markova, Yu V Korotkevich, V V Stetsenko, L P Minaeva, S A Sheveleva
We analyzed the formation of biofilms by 7 strains of Campylobacter genus bacteria and 18 strains of Enterobacteriaceae genus bacteria that were isolated from plant and animal raw materials, from finished products, and swabs from the equipment of the food industry. Biofilm formation on glass plates, slides and coverslips, microtubes made of polymeric materials and Petri dishes, and polystyrene plates of different profiles were analyzed. When studying the process of films formation, different effects on bacterial populations were simulated, including variation of growth factor composition of culture media, technique of creating of anaerobiosis, and biocide treatment (active chlorine solutions in a concentration of 100 mg/dm(3))...
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28239823/inhibitory-effect-and-mechanism-of-metformin-on-human-ovarian-cancer-cells-skov-3-and-a2780
#19
J Huo, X-H Bian, Y Huang, Z-C Miao, L-H Song
OBJECTIVE: Ovarian cancer is the most common malignant tumor in female reproductive system. Metformin is an orally taken hypoglycemic agent, which is extensively applied in the clinic. Clinical trials find that there may be a certain degree of action of the metformin in inhibiting malignant tumors. This paper aims to investigate the inhibitory effect and mechanism of metformin on human ovarian cancer cells. MATERIALS AND METHODS: Through in vitro cell experiment, the influences of metformin on the proliferation, colony formation and apoptosis of ovarian carcinoma cells were studied...
February 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28231617/characterization-of-staphylococcus-epidermidis-strains-isolated-from-industrial-cleanrooms-under-regular-routine-disinfection
#20
U Ribič, A Klančnik, B Jeršek
AIMS: The purpose of this study was the genotypic and phenotypic characterization of 57 strains of Staphylococcus epidermidis isolated from cleanroom environments, based on their biofilm formation and antimicrobial resistance profiles. METHODS AND RESULTS: Biofilm formation was investigated using real-time PCR (icaA, aap, bhp genes), the Congo red agar method, and the crystal violet assay. The majority of the strains (59.7%; 34/57) did not form biofilms according to the crystal violet assay, although the biofilm-associated genes were present in 94...
February 23, 2017: Journal of Applied Microbiology
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