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https://www.readbyqxmd.com/read/29622874/utility-of-cd157-as-a-common-leukocytes-marker-for-paroxysmal-nocturnal-hemoglobinuria-screening-in-a-single-tube-five-color-combination
#1
Khaliqur Rahman, Ruchi Gupta, Shivangi Harankhedkar, Tanvi Gupta, Manoj K Sarkar, Soniya Nityanand
To study the utility and advantage of CD157 in the paroxysmal nocturnal hemoglobinuria (PNH) screening along with its ability to replace CD24 and CD14. This was a confirmatory study to analyse the role and advantage of CD157 in a single tube five color combination to identify the PNH clones. A serial tenfold dilution experiments was carried out for sensitivity assessment. Reproducibility was checked in the intra-assay and inter-assay experiments. The results obtained with CD157 based assay were compared with the routinely used single tube six color CD24/CD14 based assay...
April 2018: Indian Journal of Hematology & Blood Transfusion
https://www.readbyqxmd.com/read/29621925/transport-kinetics-of-chromium-in-perfused-human-placental-lobule-in-late-gestation-in-vitro-study
#2
B Al-Sannan, M Nandakumaran, J Al-Harmi, M Al-Shammari, M Fouda
OBJECTIVE: Reports relating to maternal-fetal transport kinetics of chromium, an essential trace element in the human pregnancies are scanty. Hence, we thought it interesting to investigate the transport kinetics of this trace element in the human placenta in late gestation in vitro. METHODS: Human placentae were collected immediately after delivery from normal uncomplicated pregnancies. Chromium chloride solution (GFS Chem Inc, Ohio, USA) at 10 times the physiological concentrations and antipyrine (Sigma Chem Co...
April 5, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29618449/pharmacophoric-screening-of-newly-synthesized-isoniazid-derivatives-and-their-antimycobacterial-activity
#3
Sabahat Naeem, Shamim Akhtar, Zaheer Ali, Muhammad Yahya Noori, Ahsaan Ahmed
Mycobacterium tuberculosis is clinically recognized as a causative agent of Tuberculosis. Keeping in view, this study was endeavored to screen our previously synthesized seventeen INH analogues for their antimycobacterial potential using proportion method. During this process, INH and all the seventeen compounds were examined at different concentrations of 0.05, 0.1 and 0.2μg/mL which were prepared using Lowenstein-Jensen (LJ) base. For drug susceptibility test, three Mycobacterial strains ATCC H37Rv, known INH-sensitive and INH-resistant strains were selected, sub-cultured on LJ Medium and serial diluted to achieve 1:10, 1:100, 1:1000 and 1:10000 from calibrated bacterial suspension Mcfarland No...
March 2018: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29617361/a-real-time-pcr-tool-for-the-surveillance-of-zoonotic-onchocerca-lupi-in-dogs-cats-and-potential-vectors
#4
Maria Stefania Latrofa, Giada Annoscia, Vito Colella, Maria Alfonsa Cavalera, Carla Maia, Coralie Martin, Jan Šlapeta, Domenico Otranto
The ocular onchocercosis is caused is by the zoonotic parasite Onchocerca lupi (Spirurida: Onchocercidae). A major hindrance to scientific progress is the absence of a reliable diagnostic test in affected individuals. Microscopic examination of skin snip sediments and the identification of adults embedded in ocular nodules are seldom performed and labour-intensive. A quantitative real-time PCR (qPCR) assay was herein standardized for the detection of O. lupi DNA and the results compared with microscopic examination and conventional PCR (cPCR)...
April 4, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29582323/assessment-of-biotoxicity-of-cu-nanoparticles-with-respect-to-probiotic-strains-of-microorganisms-and-representatives-of-the-normal-flora-of-the-intestine-of-broiler-chickens
#5
Aleksey Nikolayevich Sizentsov, Olga Vilorievna Kvan, Elena Petrovna Miroshnikova, Irina Aleksandrovna Gavrish, Victoria Alekseevna Serdaeva, Artem Vladimirovich Bykov
Copper nanoparticle Cu (d = 55 ± 15 nm) and CuO nanoparticles (d = 90 ± 10 nm) were used in the studies (OOO Platina, Russia). Using the method of pure cultures, we extracted Lactobacillus, Enterococcus, and Enterobacterium from the intestines of broilers. Additionally, strains of Bacillus subtilis 10641 and Bifidobacterium were involved in probiotic strains. The data obtained in the course of the study testify to the insignificant biotoxicity of copper nanoparticles with respect to representatives of the genera Lactobacillus (30 to 15 μg/ml) and Bifidobacterium (30 μg/ml), with the most sensitive bacteria being the genus Lactobacillus, for which a concentration of 7...
March 26, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29576953/pcr-an-r-package-for-quality-assessment-analysis-and-testing-of-qpcr-data
#6
Mahmoud Ahmed, Deok Ryong Kim
Background: Real-time quantitative PCR (qPCR) is a broadly used technique in the biomedical research. Currently, few different analysis models are used to determine the quality of data and to quantify the mRNA level across the experimental conditions. Methods: We developed an R package to implement methods for quality assessment, analysis and testing qPCR data for statistical significance. Double Delta C T and standard curve models were implemented to quantify the relative expression of target genes from C T in standard qPCR control-group experiments...
2018: PeerJ
https://www.readbyqxmd.com/read/29576580/in-vitro-cytotoxic-evaluation-of-novel-fast-setting-calcium-silicate-cement-compositions-and-dental-materials-using-colorimetric-methyl-thiazolyl-tetrazolium-assay
#7
Bahram Ranjkesh, Flemming Isidor, David Christian Evar Kraft, Henrik Løvschall
A novel fast-setting calcium silicate cement with fluoride (CSC) has been developed for potential application in tooth crowns. This study compared the cytotoxicity of CSC compositions and a variety of dental materials. We tested CSC compositions (Protooth), MTA, Biodentine, Ketac Molar, Fuji II LC, Vitrebond, DeTrey Zinc, Dycal, and IRM, DMEM (negative control) and 1% NaOCl (positive control). After setting of cements for 24 h, specimens were immersed in DMEM for 24 h to obtain material elutes. The elutes were serially diluted in serum-free DMEM to obtain three dilutions...
2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29570739/development-of-an-improved-rt-qpcr-assay-for-detection-of-japanese-encephalitis-virus-jev-rna-including-a-systematic-review-and-comprehensive-comparison-with-published-methods
#8
Tehmina Bharucha, Onanong Sengvilaipaseuth, Manivanh Vongsouvath, Malavanh Vongsouvath, Viengmon Davong, Phonepasith Panyanouvong, Géraldine Piorkowski, Jeremy A Garson, Paul N Newton, Xavier de Lamballerie, Audrey Dubot-Pérès
BACKGROUND: Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia, and the commonest cause of mosquito-borne encephalitis worldwide. Detection of JEV RNA remains challenging due to the characteristic brief and low viraemia, with 0-25% of patients positive, and the mainstay of diagnosis remains detection of anti-JEV IgM antibody. METHODS: We performed a systematic review of published RT-PCR protocols, and evaluated them in silico and in vitro alongside new primers and probes designed using a multiple genome alignment of all JEV strains >9,000nt from GenBank, downloaded from the NCBI website (November 2016)...
2018: PloS One
https://www.readbyqxmd.com/read/29569291/in-vitro-efficacy-of-a-honey-based-gel-against-canine-clinical-isolates-of-staphylococcus-pseudintermedius-and-malassezia-pachydermatis
#9
Ana M P Oliveira, Joana S P Devesa, Peter B Hill
BACKGROUND: Staphylococcus pseudintermedius and Malassezia pachydermatis are important agents in canine pyoderma and otitis. HYPOTHESIS/OBJECTIVES: Determine the in vitro efficacy of a honey-based gel (HBO) against meticillin-susceptible S. pseudintermedius (MSSP), meticillin-resistant S. pseudintermedius (MRSP) and M. pachydermatis, by minimum bactericidal concentration (MBC), minimum fungicidal concentration (MFC) and time-kill assay (TKA). Efficacy of the product's honey component (HO) also was evaluated...
March 22, 2018: Veterinary Dermatology
https://www.readbyqxmd.com/read/29566082/a-d-enantiomer-of-the-antimicrobial-peptide-gl13k-evades-antimicrobial-resistance-in-the-gram-positive-bacteria-enterococcus-faecalis-and-streptococcus-gordonii
#10
Helmut Hirt, Jeffrey W Hall, Elliot Larson, Sven-Ulrik Gorr
Antimicrobial peptides represent an alternative to traditional antibiotics that may be less susceptible to bacterial resistance mechanisms by directly attacking the bacterial cell membrane. However, bacteria have a variety of defense mechanisms that can prevent cationic antimicrobial peptides from reaching the cell membrane. The L- and D-enantiomers of the antimicrobial peptide GL13K were tested against the Gram-positive bacteria Enterococcus faecalis and Streptococcus gordonii to understand the role of bacterial proteases and cell wall modifications in bacterial resistance...
2018: PloS One
https://www.readbyqxmd.com/read/29555416/phosphorus-kinetics-in-lambs-experimentally-infected-with-trichostrongylus-colubriformis-with-the-use-of-32-p
#11
Tairon Pannunzio Dias E Silva, Carolina Rodriguez Jimenez, Egon Ion Ieda, Adibe Luiz Abdalla, Helder Louvandini
This study was conducted to evaluate the metabolism and phosphorus (P) kinetics in lambs experimentally infected with Trichostrongylus colubriformis using the isotope dilution technique and modelling. Fifteen male lambs (21.1 ± 1.50 kg) of the Santa Inês hair breed of approximately six months old, distributed in the treatments infected (I, n = 8) and control (C, n = 7) were used. The infected lambs received serial infections with 5000 T. colubriformis larvae, three times per week, for 3 weeks (45 000 T...
March 16, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29550438/evaluating-the-impact-of-synthetic-herbicides-on-soil-dwelling-macrobes-and-the-physical-state-of-soil-in-an-agro-ecosystem
#12
J O Frimpong, E S K Ofori, S Yeboah, D Marri, B K Offei, F Apaatah, J O Sintim, E Ofori-Ayeh, M Osae
This study evaluated three herbicides active ingredients: Paraquat, Glyphosate and 2,4-D Amine in commercial formulations as Frankoquat, Roundup and Agriherb respectively under field conditions to determine their influence on soil dwelling macrobes and the physical state of soil. Herbicides were serially diluted to three treatment concentrations for each plus three controls. Herbicide concentrations were applied to the demarcated field on three consecutive occasions in splits. Macrobes extraction from soil was done under a stereo microscope at 20 × magnification...
March 15, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29547758/human-papillomavirus-type-16-integration-analysis-by-real-time-pcr-assay-in-associated-cancers
#13
Mohammad Hadi Karbalaie Niya, Hossein Keyvani, Fahimeh Safarnezhad Tameshkel, Mostafa Salehi-Vaziri, Sedigheh Teaghinezhad-S, Farah Bokharaei Salim, Seyed Hamid Reza Monavari, Davod Javanmard
Human papillomavirus (HPV) is a common viral infection worldwide associated with a variety of cancers. The integration of the HPV genome in these patients causes chromosomal instability and triggers carcinogenesis. The aim of this study was to investigate the HPV-16 genome physical status in four major cancers related to HPV infection. Formalin-fixed paraffin-embedded blocks from our previous projects on head and neck, colorectal, penile, and cervical cancers were collected, and HPV-16-positive specimens were used for further analysis...
March 13, 2018: Translational Oncology
https://www.readbyqxmd.com/read/29535804/detection-of-somatic-mutations-in-cell-free-dna-in-plasma-and-correlation-with-overall-survival-in-patients-with-solid-tumors
#14
Meenakshi Mehrotra, Rajesh R Singh, Sanam Loghavi, Dzifa Yawa Duose, Bedia A Barkoh, Carmen Behrens, Keyur P Patel, Mark J Routbort, Scott Kopetz, Russell R Broaddus, L Jeffrey Medeiros, Ignacio I Wistuba, Rajyalakshmi Luthra
A suitable clinical-grade platform is required for detection of somatic mutations with high sensitivity in cell-free DNA (cfDNA) of patients with solid tumors. In this study, we evaluated in parallel ultra-deep NGS with MassARRAY and allele-specific droplet digital PCR (ddPCR) for cfDNA genotyping and correlated cfDNA yield and mutation status with overall survival (OS) of patients. We assessed plasma samples from 46 patients with various advanced metastatic solid tumors and known mutations by deep sequencing using an Ampliseq cancer hotspot panel V2 on Ion Proton...
February 13, 2018: Oncotarget
https://www.readbyqxmd.com/read/29534739/comparison-of-two-different-combined-test-strips-with-fluorescent-microspheres-or-colored-microspheres-as-tracers-for-rotavirus-and-adenovirus-detection
#15
Na Jiang, Lei Shi, Jieping Lin, Lifang Zhang, Yanxia Peng, Huiying Sheng, Ping Wu, Qingjun Pan
BACKGROUND: Rotavirus (RV) and enteric adenovirus (AdV) mainly cause infantile infectious gastroenteritis. Several separate test methods for the detection of RV or AdV are currently available, but few tests are able to simultaneously detect both RV and AdV viruses, especially in primary medical institutions. METHODS: The present study was mainly designed to compare the performance of two combined test strips for the detection of RV and AdV: a rotavirus-adenovirus strip with fluorescent microspheres for tracers (FMT); and the CerTest rotavirus-adenovirus blister strip with colored microspheres for tracers (CMT)...
March 13, 2018: Virology Journal
https://www.readbyqxmd.com/read/29532802/in-situ-cultivation-of-previously-uncultivable-microorganisms-using-the-ichip
#16
Brittany Berdy, Amy L Spoering, Losee L Ling, Slava S Epstein
Most microbial species remain uncultivated, and modifying artificial nutrient media brings only an incremental increase in cultivability. We reasoned that an alternative way to cultivate species with unknown requirements is to use naturally occurring combinations of growth factors. To achieve this, we moved cultivation into the microbes' natural habitat by placing cells taken from varying environmental samples into diffusion chambers, which are then returned to nature for incubation. By miniaturizing the chambers and placing only one to several cells into each chamber, we can grow and isolate microorganisms in axenic culture in one step...
October 2017: Nature Protocols
https://www.readbyqxmd.com/read/29529898/improved-quantification-of-a-commercial-enzyme-linked-immunosorbent-assay-kit-for-measuring-anti-mda5-antibody
#17
Takahisa Gono, Yuka Okazaki, Akihiro Murakami, Masataka Kuwana
OBJECTIVES: To compare the quantitative performance for measuring anti-MDA5 antibody titer of two enzyme-linked immunosorbent assay (ELISA) systems: an in-house ELISA and the commercial MESACUPTM anti-MDA5 test. METHODS: Anti-MDA5 antibody titer was measured in sera from 70 patients with dermatomyositis using an in-house ELISA and the MESACUPTM anti-MDA5 test side-by-side. For the commercial ELISA kit, serum samples diluted 1:101 were used according to the manufacturer's protocol, but serial dilutions of sera were also examined to identify the optimal serum dilution for quantification...
March 13, 2018: Modern Rheumatology
https://www.readbyqxmd.com/read/29528705/inoculation-protocols-influence-the-thermal-resistance-of-salmonella-enteritidis-pt-30-in-fabricated-almond-wheat-and-date-products
#18
Pichamon Limcharoenchat, Sarah E Buchholz, Michael K James, Nicole O Hall, Elliot T Ryser, Bradley P Marks
Inoculation methods in pathogen inactivation studies ideally represent conditions that might occur in real-world scenarios. Surface contamination in or on low-moisture foods affects Salmonella thermal resistance, which is critically important for process validation applications. The objective of this study was to quantify the effect of inoculation protocol on the thermal resistance of Salmonella Enteritidis PT 30 in fabricated low-moisture foods. Almond meal, almond butter, wheat meal, wheat flour, and date paste were inoculated via prefabrication and postfabrication protocols...
March 12, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29528475/degree-of-response-to-homeopathic-potencies-correlates-with-dipole-moment-size-in-molecular-detectors-implications-for-understanding-the-fundamental-nature-of-serially-diluted-and-succussed-solutions
#19
Steven J Cartwright
BACKGROUND:  The use of solvatochromic dyes to investigate homeopathic potencies holds out the promise of understanding the nature of serially succussed and diluted solutions at a fundamental physicochemical level. Recent studies have shown that a range of different dyes interact with potencies and, moreover, the nature of the interaction is beginning to allow certain specific characteristics of potencies to be delineated. AIMS AND METHODS:  The study reported in this article takes previous investigations further and aims to understand more about the nature of the interaction between potencies and solvatochromic dyes...
February 2018: Homeopathy: the Journal of the Faculty of Homeopathy
https://www.readbyqxmd.com/read/29512604/-in-vitro-susceptibility-of-indian-plasmodium-falciparum-isolates-to-different-antimalarial-drugs-antibiotics
#20
Pooja Agarwal, A R Anvikar, C R Pillai, Kumkum Srivastava
Background & objectives: : The in vitro assays for susceptibility of Plasmodium falciparum to antimalarial drugs are important tools for monitoring drug resistance. During the present study, efforts were made to establish long-term continuous in vitro culture of Indian field isolates of P. falciparum and to determine their sensitivity to standard antimalarial drugs and antibiotics. Methods: Four (MZR-I, -II, -III and -IV) P. falciparum isolates were obtained from four patients who showed artemisinin-based combination therapy (ACT) from Mizoram, a north-eastern State of India, and characterized for their in vitro susceptibility to chloroquine diphosphate (CQ), quinine hydrochloride dehydrate, mefloquine, piperaquine, artemether, arteether, dihydro-artemisinin (DHA), lumefantrine and atovaquone and antibiotics, azithromycin and doxycycline...
November 2017: Indian Journal of Medical Research
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