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https://www.readbyqxmd.com/read/28316949/evaluation-of-the-role-of-probiotics-in-endodontic-treatment-a-preliminary-study
#1
Aarti Bohora, Sharad Kokate
AIMS AND OBJECTIVES: The principal goal of endodontics is the prevention of periapical infection. Acute and chronic apical periodontitis occur due to the persistence of pathogenic microorganisms such as Enterococcus faecalis and Candida albicans harboring the root canal systems of the teeth. The concept of the use of probiotics in addressing endodontic disease is new and has not been studied adequately. On the basis of the success of probiotics in periodontal treatment, this preliminary work was performed (a) to evaluate the antibacterial efficacy of probiotics against common endodontic pathogens, i...
January 2017: Journal of International Society of Preventive & Community Dentistry
https://www.readbyqxmd.com/read/28299849/erythrocyte-osmotic-fragility-and-general-health-status-of-adolescent-sprague-dawley-rats-supplemented-with-hibiscus-sabdariffa-aqueous-calyx-extracts-as-neonates-followed-by-a-high-fructose-diet-post-weaning
#2
K G Ibrahim, B W Lembede, E Chivandi, K Erlwanger
High-fructose diets (HFD) can cause oxidative damage to tissues including erythrocyte cell membranes. Hibiscus sabdariffa (HS) has protective antioxidant properties. Rats were used to investigate whether the consumption of HS by neonates would result in long-term effects on their erythrocyte osmotic fragility (EOF) and general health when later fed a high-fructose diet post-weaning through adolescence. Eighty of four-day-old Sprague Dawley rat pups were divided randomly into three treatment groups. The controls (n = 27) received distilled water at 10 ml/kg b...
March 16, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28298816/effect-of-gallium-arsenic-laser-on-photosensitized-periodontopathic-anaerobic-organisms-an-in-vitro-study
#3
Setu Mathur, Shaila Veerappa Kothiwale, Buddhi Prakash Nag, Tanu Mathur, Ashoka Bhansali, Rohit Kumar Khatri
BACKGROUND: The mainstay of periodontal therapy is mechanical removal of subgingival plaque. There is considerable interest in supplementing it with the use of antibiotics and antiseptics. Many drawbacks are associated with these adjunctive pharmacological regimens such as development of resistance to antibiotics and disruption of microflora of the gastrointestinal tract. Hence, alternate means of killing subgingival bacteria are clearly desirable. One such method is the use of laser...
July 2016: Journal of Indian Society of Periodontology
https://www.readbyqxmd.com/read/28293307/sensitivity-to-antimicrobial-drugs-of-pseudomonas-aeruginosa-extreme-resistant-strains-isolated-in-the-major-hospitals-of-central-kazakhstan
#4
Ilya S Azizov, Alyona V Lavrinenko, Ilya A Belyaev, Dmitry B Babenko, Natalya A Shambilova, Nelya M Bissenova
AIM: The article presents the current data on the sensitivity of the main 37 strains of eXtremaly Drugs Resistance (XDR) category to anti-pseudomonas drugs. MATERIAL AND METHODS: The strains were collected during the prospective multicenter study in large multidisciplinary hospitals of Central Kazakhstan. Susceptibility to antimicrobial drugs was carried out by disk method and the serial dilution method with the interpretation of the results according to EUCAST criteria...
March 15, 2017: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/28291433/effects-of-bone-cement-loaded-with-teicoplanin-n-acetylcysteine-or-their-combination-on-staphylococcus-aureus-biofilm-formation-an-in-vitro-study
#5
Hasan Göçer, Dilek Emir, Mehmet Emin Önger, Nevzat Dabak
OBJECTIVES: This study aims to demonstrate the antibiofilm effects of teicoplanin alone, N-acetyl cysteine (NAC) alone, or combination of both compounds when mixed with bone cement. MATERIALS AND METHODS: A total of four groups were formed by using six cement samples in each, prepared with bone cement having different contents in each group. Group 1 (control group): cement alone without any drugs added. Group 2: 40 g cement, 400 mg teicoplanin. Group 3: 40 g cement, 6 g NAC...
April 2017: Eklem Hastalıkları Ve Cerrahisi, Joint Diseases & related Surgery
https://www.readbyqxmd.com/read/28281929/in-vitro-amplification-of-h-type-atypical-bovine-spongiform-encephalopathy-by-protein-misfolding-cyclic-amplification
#6
Matthew J O'Connor, Keith Bishop, Robert G Workman, Ben C Maddison, Kevin C Gough
The in vitro amplification of prions by serial protein misfolding cyclic amplification has been shown to detect PrP(Sc) to levels at least as sensitive as rodent bioassay but in a fraction of the time. Bovine spongiform encephalopathy is a zoonotic prion disease in cattle and has been shown to occur in 3 distinct forms, classical BSE (C-BSE) and 2 atypical BSE forms (L-BSE and H-BSE). Atypical forms are usually detected in asymptomatic, older cattle and are suggested to be spontaneous forms of the disease. Here, we show the development of a serial protein misfolding cyclic amplification method for the detection of H-BSE...
January 2, 2017: Prion
https://www.readbyqxmd.com/read/28271692/colorimetric-detection-of-23-human-papillomavirus-genotypes-by-loop-mediated-isothermal-amplification
#7
Junxiao Lin, Biao Ma, Jiehong Fang, Ye Wang, Haizhen He, Wei Lin, Wei Su, Mingzhou Zhang
BACKGROUND: Human papillomavirus (HPV) infection is linked to cervical cancer. With the technological development of molecular biology and epidemiology, detection and treatment of HPV has become an important mean to prevent cervical cancer. METHODS: A simple, rapid, and sensitive colorimetric loop-mediated isothermal amplification (LAMP) method was established herein to detect 23 HPV genotypes. The sequences of the primers for the LAMP reaction were located in the L1 gene of the HPV genome...
March 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28268092/droplet-digital-pcr-is-a-reliable-tool-for-monitoring-minimal-residual-disease-in-acute-promyelocytic-leukemia
#8
Claudia Brunetti, Luisa Anelli, Antonella Zagaria, Angela Minervini, Crescenzio F Minervini, Paola Casieri, Nicoletta Coccaro, Cosimo Cumbo, Giuseppina Tota, Luciana Impera, Paola Orsini, Giorgina Specchia, Francesco Albano
Nested PCR (nPCR) and real-time quantitative PCR (qPCR) are well-established methods for monitoring minimal residual disease (MRD) in acute promyelocytic leukemia (APL). Despite their remarkable sensitivity and specificity, both methods have inherent limitations, such as qualitative MRD evaluation and relative quantification. Herein, we used droplet digital PCR (ddPCR) to monitor MRD in 21 APL patients and compared its performance with nPCR and qPCR. After assessing the limit of detection (LOD) for each technique on serial dilutions of PML-RARA bcr1 and bcr3 transcripts, a total of 48 follow-up samples were analyzed and the results compared...
March 4, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28267558/rapid-detection-of-donor-cell-free-dna-in-lung-transplant-recipients-with-rejections-using-donor-recipient-hla-mismatch
#9
Jun Zou, Brian Duffy, Michael Slade, Andrew Lee Young, Nancy Steward, Ramsey Hachem, T Mohanakumar
Fiberoptic bronchoscopy and transbronchial lung biopsy are currently the gold standard for detection of acute rejection following human lung transplantation (LTx). However, these surveillance procedures are expensive and invasive. Up to now, there are few new methods that have demonstrated clinical utility for detecting early stages of rejection following human lung transplantation. We optimized and technically validated a novel method to quantify donor-derived circulating cell free DNA (DcfDNA) that can be used as an early biomarker for lung allograft rejection...
March 4, 2017: Human Immunology
https://www.readbyqxmd.com/read/28267427/prevention-of-biomaterial-infection-by-pre-operative-incubation-with-human-cells
#10
Ramón Pérez-Tanoira, Antti A Aarnisalo, Kari K Eklund, Xia Han, Antti Soininen, Veli-Matti Tiainen, Jaime Esteban, Teemu J Kinnari
BACKGROUND: Cells of tissues and biofilm forming bacteria compete for the living space on the surface of an implant. We hypothesized the incubation of the implant (titanium, polydimethylsiloxane, and polystyrene surface) with human cells before implantation as a strategy to prevent bacterial adhesion and biofilm formation. METHODS: After 24 hours of incubation with human osteogenic sarcoma SaOS-2 cells (1 × 10(5) cells/mL), the materials were incubated for 4...
March 7, 2017: Surgical Infections
https://www.readbyqxmd.com/read/28257256/biological-effects-of-provisional-resin-materials-on-human-dental-pulp-stem-cells
#11
S-K Jun, C Mahapatra, H-H Lee, H-W Kim, J-H Lee
OBJECTIVES: This study investigated the in vitro cytotoxicity as well as the proinflammatory cytokine expression of provisional resin materials on primary cultured human dental pulp stem cells (hDPSCs). METHODS: Five commercially available provisional resin materials were chosen (SNAP [SN], Luxatemp [LT], Jet [JE], Revotek LC [RL], and Vipi block [VB]). Eluates that were either polymerizing or already set were added to hDPSCs under serially diluted conditions divided into three different setting times (25% set, 50% set, and 100% set) and incubated for 24 hours with 2× concentrated culture media...
March 2017: Operative Dentistry
https://www.readbyqxmd.com/read/28254776/accurate-quantification-of-site-specific-acetylation-stoichiometry-reveals-the-impact-of-sirtuin-deacetylase-cobb-on-the-e-coli-acetylome
#12
Brian Tate Weinert, Shankha Satpathy, Bogi Karbech Hansen, David Lyon, Lars Juhl Jensen, Chunaram Choudhary
Lysine acetylation is a protein posttranslational modification (PTM) that occurs on thousands of lysine residues in diverse organisms from bacteria to humans. Accurate measurement of acetylation stoichiometry on a proteome-wide scale remains challenging. Most methods employ a comparison of chemically acetylated peptides to native acetylated peptides, however, the potentially large differences in abundance between these peptides presents a challenge for accurate quantification. Stable isotope labeling by amino acids in cell culture (SILAC)-based mass spectrometry (MS) is one of the most widely used quantitative proteomic methods...
March 2, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28252872/powerplex%C3%A2-fusion-6c-system-evaluation-study-for-analysis-of-casework-and-database-samples
#13
Selena Cisana, Nicoletta Cerri, Alessandro Bosetti, Andrea Verzeletti, Venusia Cortellini
AIM: To report on the successful analysis of amplicons obtained with PowerPlex® Fusion 6C System, a highly robust 27-plex genotyping kit developed for human identification laboratories, on the Applied Biosystems® 3500 Genetic Analyzer. METHOD: We performed characterization and evaluation studies following the Scientific Working Group on DNA Analysis Methods (SWGDAM) validation guidelines, examining several critical areas of kit performance. We report the results of sensitivity, robustness, heterozygous peak height ratio, precision, concordance, caseworks, and mixture interpretations...
February 28, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28247312/interference-in-acth-immunoassay-negatively-impacts-the-management-of-subclinical-hypercortisolism
#14
Serkan Yener, Leyla Demir, Mustafa Demirpence, Mustafa Mahmut Baris, Ilgin Yildirim Simsir, Secil Ozisik, Abdurrahman Comlekci, Tevfik Demir
PURPOSE: Low plasma corticotropin is considered a useful parameter for the diagnosis of subclinical hypercortisolism in patients with an adrenal incidentaloma. However, immunoassays are vulnerable to interference from endogenous antibodies. In this study, subjects who underwent Hypothalamus-pituitary-adrenal axis evaluation for the assessment of subclinical hypercortisolism were evaluated. The objective of the study was to ascertain whether antibody interference in corticotropin immunoassay affected the diagnostic work-up and clinical decisions...
February 28, 2017: Endocrine
https://www.readbyqxmd.com/read/28242424/should-we-care-about-transmission-of-infectious-bacterial-agents-through-serum-handling
#15
Pezhman Mahmoodi, Abdolmajid Mohammadzadeh, Shadi Baniardalani, Alireza Nourian, Ali Sadeghi-Nasab, Maryam Khaninour
Immunological assays are valuable diagnostic tools to detect infections due to most of bacterial microorganisms. However, the question is how much safe are the common serum samples used as sources of antibodies in these assays? Here, we experimentally followed the issue. 10-fold serial dilutions of two Gram-positive, Staphylococcus aureus and Bacillus cereus, and one Gram-negative bacteria, Escherichia coli, were prepared and spiked into freshly taken blood samples of human and four animal species including sheep, goat, cattle, and horse...
February 24, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28242337/survival-of-vaginal-microorganisms-in-three-commercially-available-transport-systems
#16
Allison L DeMarco, Lorna K Rabe, Michele N Austin, Kevin A Stoner, Hilary A Avolia, Leslie A Meyn, Sharon L Hillier
Transport systems are used to collect and maintain the viability of microorganisms. Two Amies media based transport systems, BD CultureSwab™ MaxV(+) Amies Medium without Charcoal (MaxV(+)) and Fisherfinest(®) with Amies gel Transport Medium without charcoal (Fisherfinest(®)) were compared to a Cary-Blair media based transport system, Starswab(®) Anaerobic Transport System (Starswab(®)), for their capacity to maintain the viability of 17 clinical microorganisms commonly isolated from the vagina (Lactobacillus crispatus, L...
February 24, 2017: Anaerobe
https://www.readbyqxmd.com/read/28241818/the-antibacterial-activity-of-extracts-of-nine-plant-species-with-good-activity-against-escherichia-coli-against-five-other-bacteria-and-cytotoxicity-of-extracts
#17
Ishaku Leo Elisha, Francien S Botha, Lyndy Joy McGaw, Jacobus Nicolaas Eloff
BACKGROUND: The development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal health and their prolonged use as growth promoters at sub-clinical doses in poultry and livestock production. We were interested in investigating plants that could be useful in protecting humans or animals against diarrhoea. We decided to work on extracts of nine plant species with good activity against Escherichia coli based on earlier work in the Phytomedicine Programme...
February 28, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28235402/anti-mycobacteria-potential-and-synergistic-effects-of-combined-crude-extracts-of-selected-medicinal-plants-used-by-bapedi-traditional-healers-to-treat-tuberculosis-related-symptoms-in-limpopo-province-south-africa
#18
Nancy Patience Motlalepula Komape, Victor Patrick Bagla, Prudence Kabongo-Kayoka, Peter Masoko
BACKGROUND: Tuberculosis is an infectious communicable disease and the causative agent of the disease has over the years developed resistance to streamline chemotherapeutic agents with dire consequences and there is a need for development of new and more potent alternatives. METHODS: Constituents of leaves material of Combretum heroroense, Citrus lemon and Apodytes dimidiata were serially extracted using solvents of varying polarity. TLC finger print profile of the different extracts were determined by spraying eluted plates with vanillin sulphuric acid and 2, 2- diphenylpicryl hydrazyl (DPPH) for the presence of antioxidant constituents...
February 24, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28223973/a-perspective-study-of-koumiss-microbiome-by-metagenomics-analysis-based-on-single-cell-amplification-technique
#19
Guoqiang Yao, Jie Yu, Qiangchuan Hou, Wenyan Hui, Wenjun Liu, Lai-Yu Kwok, Bilige Menghe, Tiansong Sun, Heping Zhang, Wenyi Zhang
Koumiss is a traditional fermented dairy product and a good source for isolating novel bacteria with biotechnology potential. In the present study, we applied the single-cell amplification technique in the metagenomics analysis of koumiss. This approach aimed at detecting the low-abundant bacteria in the koumiss. Briefly, each sample was first serially diluted until reaching the level of approximately 100 cells. Then, three diluted bacterial suspensions were randomly picked for further study. By analyzing 30 diluted koumiss suspensions, a total of 24 bacterial species were identified...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28222680/field-evaluation-of-a-recombinase-polymerase-amplification-assay-for-the-diagnosis-of-schistosoma-japonicum-infection-in-hunan-province-of-china
#20
Weiwei Xing, Xinling Yu, Jingtao Feng, Kui Sun, Wenliang Fu, Yuanyuan Wang, Minji Zou, Wenrong Xia, Zhihong Luo, Hongbin He, Yuesheng Li, Donggang Xu
BACKGROUND: Current diagnostic methods for Schistosoma japonicum infection are insensitive for low-density infections. Therefore, a new diagnostic assay based on recombinase polymerase amplification (RPA) technology was established and assessed for field applification. METHODS: The S.japonicum RPA assay was developed to target highly repetitive retrotransposon SjR2 gene of S japonicum, and its sensitivity and specificity were assessed by serial dilution of S. japonicum genomic DNA and other related worm genomic DNA respectively...
February 21, 2017: BMC Infectious Diseases
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