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Sally V Irwin, Peter Fisher, Emily Graham, Ashley Malek, Adriel Robidoux
Sulfites and other preservatives are considered food additives to limit bacterial contamination, and are generally regarded as safe for consumption by governmental regulatory agencies at concentrations up to 5000 parts per million (ppm). Consumption of bactericidal and bacteriostatic drugs have been shown to damage beneficial bacteria in the human gut and this damage has been associated with several diseases. In the present study, bactericidal and bacteriostatic effects of two common food preservatives, sodium bisulfite and sodium sulfite, were tested on four known beneficial bacterial species common as probiotics and members of the human gut microbiota...
2017: PloS One
Ran Zhuo, Joanne Cho, YuanYuan Qiu, Brendon D Parsons, Bonita E Lee, Linda Chui, Stephen B Freedman, Xiaoli Pang
BACKGROUND: It is important to understand the diagnostic accuracy of multiplex panels such as the Luminex xTAG(®) Gastrointestinal Pathogen Panel (GPP) as they are increasingly employed for routine diagnostics worldwide. Recent evaluations in our laboratory identified lower detection rates of norovirus genogroup II (NoV GII) using GPP compared to our laboratory-developed RT-qPCR, Gastroenteritis Virus Panel (GVP). OBJECTIVES: To characterize the cases of discordant NoV GII results between GPP and GVP and determine the sensitivity of the two assays for specific NoV GII genotypes...
October 9, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Fiaz Alam, Qazi Najam Us Saqib
Zanthoxylum armatum fruits are used traditionally as a spice in various food preparations. The aim of this study was analysis of antimicrobial, cytotoxic, phytotoxic, insecticidal, and anti-leishmanial activity. The crude extract showed 86 ± 10% antifungal activity (Agar tube dilution method) against Trichophyton longifusis while n-hexane, chloroform, and aqueous-methanol fractions inhibited this pathogen by 90 ± 7, 85 ± 10 and 70 ± 9% respectively. The n-hexane and aqueous-methanol fraction also, respectively, showed 40 ± 10 and 87 ± 9% inhibition of Microsporum canis...
October 2017: Journal of Traditional and Complementary Medicine
Karumutchu Sitalu, B Hari Babu, J Naveena Lavanya Latha, A Lakshmana Rao
BACKGROUND: Caffeine, 1, 3, 7-trimethylxanthine is one of the xanthine derivatives that are for the most part utilized as a part of solutions as diuretics. The Cu (II) complexes have been synthesized from the N-heterocyclic carbene ligands. MATERIALS AND METHODS: The Cu (II) NHC complexes were characterized using analytical and spectral techniques. Antibacterial and antifungal activities of the Cu (II) NHC complexes were determined using the reported techniques...
2017: Pakistan Journal of Biological Sciences: PJBS
Yu-En Shih, Yu-Chih Lin, Tse-Yu Chung, Mei-Chun Liu, Guan-Heng Chen, Chia-Chang Wu, Jason T C Tzen
Astringency, a sensory characteristic of food and beverages rich in polyphenols, mainly results from the formation of complexes between polyphenols and salivary proteins, causing a reduction of the lubricating properties of saliva. To develop an in vitro assay to estimate the astringency of oolong tea infusion, artificial oil bodies were constituted with sesame oil sheltered by a modified caleosin fused with histatin 3, one of the human salivary small peptides. Aggregation of artificial oil bodies was induced when they were mixed with oolong tea infusion or its major polyphenolic compound, (-)-epigallocatechin gallate (EGCG) of 100μM as observed in light microscopy...
October 2017: Journal of Food and Drug Analysis
B J Jacques, T J Bourret, J J Shaffer
Flies are known to be mechanical vectors of bacterial, viral, and parasitic diseases. Although flies are known to transmit disease, the effects of cleaning behavior have not been well studied. This study quantified the cleaning effectiveness and behavior of three fly species: Sarcophaga bullata, Musca domestica L., and Drosophila virilis. Flies were transferred to plates of Escherichia coli or Pseudomonas aeruginosa and allowed to walk on the bacteria for a total of 5 min. After the flies were contaminated, they were either immediately collected to quantify bacteria or were placed onto sterile plates to clean for 5 or 10 min...
July 14, 2017: Journal of Medical Entomology
Muyun Peng, Chen Chen, Alicia Hulbert, Malcolm V Brock, Fenglei Yu
Tumor DNA contains specific somatic alterations that are crucial for the diagnosis and treatment of cancer. Due to the spatial and temporal intra-tumor heterogeneity, multi-sampling is needed to adequately characterize the somatic alterations. Tissue biopsy, however, is limited by the restricted access to sample and the challenges to recapitulate the tumor clonal diversity. Non-blood circulating tumor DNA are tumor DNA fragments presents in non-blood body fluids, such as urine, saliva, sputum, stool, pleural fluid, and cerebrospinal fluid (CSF)...
September 15, 2017: Oncotarget
A R E O Xavier, E R Lima, A M E Oliveira, L Cardoso, J Santos, C H C Cangussu, L N Leite, M C L Quirino, I G C Júnior, D A Oliveira, M A S Xavier
The microorganisms are the best source of extracellular enzymes since they allow an economical technology with low-resource consumption compared to animals and plants. The amylases are among the most important enzymes being the genus Bacillus one of the most investigated due to its ability to produce this enzyme. The objective of this study was to isolate and analyze the genetic diversity among bacteria of the genus Bacillus sp producer of amylase originated from the soil. To this end, soil samples were collected and submitted to the condition of extreme temperature...
September 27, 2017: Genetics and Molecular Research: GMR
Suchismita Panda, Anuva Mishra, Manoranjan Jena, Sashi Bhusan Rout, Srikrushna Mohapatra
INTRODUCTION: Anaemia is one of the common complications associated with Chronic Kidney Disease (CKD) responsible for the increase in the morbidity and mortality in such patients. Several factors have been attributed to cause renal anaemia, amongst which hyperparathyroidism is one of the less recognised reasons. Most studies have been conducted in this regard in CKD patients undergoing haemodialysis. The level of PTH in early stages of chronic kidney disease has not been much studied...
August 2017: Journal of Clinical and Diagnostic Research: JCDR
A Doescher, U Loges, E K Petershofen, T H Müller
BACKGROUND AND OBJECTIVES: Enumeration of residual white blood cells in leucoreduced blood components is essential part of quality control. Digital PCR has substantially facilitated quantitative PCR and was thus evaluated for measurements of leucocytes. MATERIALS AND METHODS: Target for quantification of leucocytes by digital droplet PCR was the blood group gene RHCE. The SPEF1 gene was added as internal control for the entire assay starting with automated DNA extraction...
October 2, 2017: Vox Sanguinis
C Venâncio, R Pereira, A C Freitas, T A P Rocha-Santos, J P da Costa, A C Duarte, I Lopes
Soil salinization, as the combination of primary and secondary events, can adversely affect organisms inhabiting this compartment. In the present study, the effects of increased salinity were assessed in four species of terrestrial fungi: Lentinus sajor caju, Phanerochaete chrysosporium, Rhizopus oryzae and Trametes versicolor. The mycelial growth and biochemical composition of the four fungi were determined under three exposure scenarios: 1) exposure to serial dilutions of natural seawater (SW), 2) exposure to serial concentrations of NaCl (potential surrogate of SW); and 3) exposure to serial concentrations of NaCl after a period of pre-exposure to low levels of NaCl...
September 27, 2017: Environmental Pollution
Alaya Mikalauskas, Michael D Parkins, Keith Poole
Objectives: Rifampicin potentiates the activity of aminoglycosides (AGs) versus Pseudomonas aeruginosa by targeting the AmgRS two-component system. In this study we examine the impact of rifampicin on the AG susceptibility of cystic fibrosis (CF) lung isolates of P. aeruginosa and the contribution of AmgRS to AG resistance in these isolates. Methods: amgR deletion derivatives of clinical isolates were constructed using standard gene replacement technology. Susceptibility to AGs ± rifampicin (at ½ MIC) was assessed using a serial 2-fold dilution assay...
September 5, 2017: Journal of Antimicrobial Chemotherapy
Danillo Lucas Alves Esposito, Benedito Antonio Lopes da Fonseca
INTRODUCTION: Chikungunya fever is a condition resulting from infection by chikungunya virus (CHIKV), an Aedes sp.-transmitted virus. This disease has been diagnosed in thousands of cases in the Americas, particularly in Brazil, in recent years, and there is an ongoing epidemic of chikungunya fever in Brazil that began in 2014. Clinical diagnosis is difficult; only a few cases have been confirmed by laboratory tests due to the low number of specific, efficient tests available for virus or antibody detection...
July 2017: Revista da Sociedade Brasileira de Medicina Tropical
Keum-Soo Song, Satish Balasaheb Nimse, Mukesh Digambar Sonawane, Yahui Lin, Zhou Zhou, Taisun Kim
A glass fibre membrane platform that allows quantification of circulating cTnT with a LoD of 0.87 pg mL(-1) is described. The proposed platform uses a glass fibre membrane, DNA-guided detection method, and antibody-conjugated fluorescent beads for the quantification of cTnT in the analytical detection range of 1-120 pg mL(-1) at room temperature in 30 min. Glass fibre membranes were chemically modified to immobilize the oligonucleotide probes that catch a biomolecular complex (FB-dAB-cTnT-cAB-DNA) containing complementary oligonucleotides...
September 27, 2017: Analyst
Yaqi Zhang, Baonian Liu, Chengchen Shao, Hongmei Xu, Aimin Xue, Ziqin Zhao, Yiwen Shen, Qiqun Tang, Jianhui Xie
The use of messenger RNA (mRNA) profiling is considered a promising method in the identification of forensically relevant body fluids which can provide crucial information for reconstructing a potential crime. However, casework samples are usually of limited quantity or have been subjected to degradation, which requires improvement of body fluid identification. Circular RNAs (circRNAs), a class of products from the backsplicing of pre-mRNAs, are shown to have high abundance, remarkable stability, and cell type-specific expression in human cells...
September 25, 2017: International Journal of Legal Medicine
Marijke W de Bar, Ellen C Hopmans, Monique Verweij, Denise J C Dorhout, Jaap S Sinninghe Damsté, Stefan Schouten
We have compared and assessed the suitability of several chromatographic methods for the analysis of long chain alkenones and long chain diols and the associated paleotemperature proxies (U(K')37 and LDI). We evaluated the traditional methods for the analysis of the U(K')37 and the LDI, gas chromatography (GC) - flame ionization detection (FID) and GC mass spectrometry (MS) using selected ion monitoring (SIM), respectively, and developed a new method using GC-MS/MS in multiple reaction monitoring mode (MRM) for the analysis of long chain diols as well as a method for automatic silylation of diols using a robot autosampler...
September 19, 2017: Journal of Chromatography. A
Fei Liu, Jiao Lan, Wei Jiao, Xianglan Mo, Yongta Huang, Huilan Ye, Ruiping Xiao, Yongli Wang, Mingzheng Mo, Liwei Shi
The present study aimed to determine the association between changes in Zbtb7a expression levels and heterogeneity of nasopharyngeal carcinoma (NPC) CNE3 sublines. CNE3 sublines were established by screening of serial dilution and continuous passage. Proliferative ability and tumorigenicity of the sublines were analyzed separately by soft-agar colony formation and mouse studies. The NPC tissues from mice were analyzed by histological evaluation and immunohistochemistry. The expression levels of Zbtb7a mRNA and protein were analyzed separately by quantitative reverse transcription polymerase chain reaction and western blotting...
September 2017: Oncology Letters
Meryem Lehbili, Abdulmagid Alabdul Magid, Ahmed Kabouche, Laurence Voutquenne-Nazabadioko, Amin Abedini, Hamid Morjani, Sophie C Gangloff, Zahia Kabouche
From the aerial parts of Salvia barrelieri Etl. (Lamiaceae), 12 compounds including a new triterpene, 3β-acetoxy-olean-18-ene-2α-ol, were isolated. Their structures were established by the combination analyses of spectroscopy including 1D-, 2D-NMR and HRESIMS and in comparison with the reported data in the literature. The antibacterial activity of these compounds was evaluated by bioautography on Staphylococcus aureus followed by the determination of MIC values by serial dilution technique against five bacteria...
September 19, 2017: Natural Product Research
Jee Sun Min, Jiseon Kim, Jung Ho Kim, Doyun Kim, Yu Fen Zheng, Ji Eun Park, Wooin Lee, Soo Kyung Bae
A highly sensitive and rapid LC-MS/MS method was developed and validated to determine the levels of carfilzomib in mice plasma by using chlorpropamide as an internal standard. Carfilzomib and chlorpropamide were extracted from 5 μL of plasma after protein precipitation with acetonitrile. Chromatographic separation was performed on Phenomenex Luna C18 column (50×2.0mm id, 3μm). The mobile phase consisted of 0.1% formic acid in acetonitrile -0.1% formic acid in water (1:1v/v) and the flow rate was 0.3mL/min...
September 1, 2017: Journal of Pharmaceutical and Biomedical Analysis
Albert K Y Tsui, Dylan Thomas, Alison Hunt, Mathew Estey, Cathy-Lou Christensen, Trefor Higgins, Irwindeep Sandhu, Karina Rodriguez-Capote
BACKGROUND: Serum protein electrophoresis (SPE) and immunofixation electrophoresis (IFE) are used in the diagnosis and monitoring of plasma cell dyscrasias. IFE is considered the most sensitive method for the detection of monoclonal proteins (M-proteins), but it is not quantitative. The goal of this study was to establish the analytical sensitivity and diagnostic performance of SPE on the Sebia Hydrasys using HYDRAGEL 30 PROTEIN(E) β1-β2. METHODOLOGY: Patient sera with a previously identified M-protein (IgG, IgA or IgM) were serially diluted with a normal serum pool and electrophoresed on the Sebia Hydrasys using HYDRAGEL 30 PROTEIN(E) β1-β2...
September 12, 2017: Clinical Biochemistry
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