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https://www.readbyqxmd.com/read/28636636/sensiscreen%C3%A2-kras-exon-2-sensitive-simplex-and-multiplex-real-time-pcr-based-assays-for-detection-of-kras-exon-2-mutations
#1
Alice Riva, Michael BØrgesen, Mariann Guldmann-Christensen, Majbritt Hauge Kyneb, Kirsten Voogd, Christina Andersen, Samantha Epistolio, Elisabetta Merlo, Tine Yding Wolff, Stephen Hamilton-Dutoit, Jan Lorenzen, Ulf Bech Christensen, Milo Frattini
Activating mutations in codon 12 and codon 13 of the KRAS (Kirsten rat sarcoma viral oncogene homolog) gene are implicated in the development of several human cancer types and influence their clinical evaluation, treatment and prognosis. Numerous different methods for KRAS genotyping are currently available displaying a wide range of sensitivities, time to answer and requirements for laboratory equipment and user skills. Here we present SensiScreen® KRAS exon 2 simplex and multiplex CE IVD assays, that use a novel real-time PCR-based method for KRAS mutation detection based on PentaBase's proprietary DNA analogue technology and designed to work on standard real-time PCR instruments...
2017: PloS One
https://www.readbyqxmd.com/read/28629407/antibacterial-activity-of-crude-extracts-of-some-south-african-medicinal-plants-against-multidrug-resistant-etiological-agents-of-diarrhoea
#2
Mary A Bisi-Johnson, Chikwelu L Obi, Babatunde B Samuel, Jacobus N Eloff, Anthony I Okoh
BACKGROUND: This study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the Eastern Cape Province, South Africa. METHODS: The acetone extracts of Acacia mearnsii De Wild., Aloe arborescens Mill., A. striata Haw., Cyathula uncinulata (Schrad.) Schinz, Eucomis autumnalis (Mill.) Chitt., E. comosa (Houtt.) Wehrh., Hermbstaedtia odorata (Burch. ex Moq.) T.Cooke, Hydnora africana Thunb, Hypoxis latifolia Wight, Pelargonium sidoides DC, Psidium guajava L and Schizocarphus nervosus (Burch...
June 19, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28628728/characterizing-the-detection-threshold-for-optical-imaging-in-surgical-oncology
#3
Andrew C Prince, Aditi Jani, Melissa Korb, Kiranya E Tipirneni, Benjamin B Kasten, Eben L Rosenthal, Jason M Warram
BACKGROUND AND OBJECTIVES: Optical imaging to guide cancer resections is rapidly transitioning into the operating room. However, the sensitivity of this technique to detect subclinical disease is yet characterized. The purpose of this study was to determine the minimum range of cancer cells that can be detected by antibody-based fluorescence imaging. METHODS: 2LMP (breast), COLO-205 (colon), MiaPaca-2 (pancreas), and SCC-1 (head and neck) cells incubated in vitro with cetuximab-IRDye800CW (dose range 8...
June 19, 2017: Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28627822/development-of-a-one-step-reverse-transcription-loop-mediated-isothermal-amplification-system-for-a-highly-sensitive-detection-of-rabbit-hepatitis-e-virus
#4
Jing Wang, Hua Zhang, Shengyuan Yan, Yichao Wang, Xin Lu, Hongwei Fu
BACKGROUND: Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in areas with poor sanitation. Rabbit is one of important animal reservoirs of the virus. Reverse transcription-loop-mediated isothermal amplification (RT-LAMP) system is a novel nucleic acid amplification assay, for which the specificity and sensitivity are much higher than that of conventional PCR. However, previously reported RT-LAMP system cannot detect rabbit HEV, which was identified recently and seems to be another potential source of human HEV infection...
May 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28625519/multiplex-ultrasensitive-genotyping-of-patients-with-non-small-cell-lung-cancer-for-epidermal-growth-factor-receptor-egfr-mutations-by-means-of-picodroplet-digital-pcr
#5
Masaru Watanabe, Tomoya Kawaguchi, Shun-Ichi Isa, Masahiko Ando, Akihiro Tamiya, Akihito Kubo, Hideo Saka, Sadanori Takeo, Hirofumi Adachi, Tsutomu Tagawa, Osamu Kawashima, Motohiro Yamashita, Kazuhiko Kataoka, Yukito Ichinose, Yukiyasu Takeuchi, Katsuya Watanabe, Akihide Matsumura, Yasuhiro Koh
Epidermal growth factor receptor (EGFR) mutations have been used as the strongest predictor of effectiveness of treatment with EGFR tyrosine kinase inhibitors (TKIs). Three most common EGFR mutations (L858R, exon 19 deletion, and T790M) are known to be major selection markers for EGFR-TKIs therapy. Here, we developed a multiplex picodroplet digital PCR (ddPCR) assay to detect 3 common EGFR mutations in 1 reaction. Serial-dilution experiments with genomic DNA harboring EGFR mutations revealed linear performance, with analytical sensitivity ~0...
June 7, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28622712/evaluation-of-the-antibacterial-and-modulatory-potential-of-%C3%AE-bisabolol-%C3%AE-cyclodextrin-and-%C3%AE-bisabolol-%C3%AE-cyclodextrin-complex
#6
Fernanda de Sousa Oliveira, Thiago Sampaio de Freitas, Rafael Pereira da Cruz, Maria do Socorro Costa, Raimundo Luiz Silva Pereira, Lucindo José Quintans-Júnior, Tatianny de Araújo Andrade, Paula Dos Passos Menezes, Bruna Maria Hipólito de Sousa, Paula Santos Nunes, Mairim Russo Serafini, Irwin Rose Alencar de Menezes, Adriano Antunes de Souza Araújo, Henrique Douglas Melo Coutinho
The present study aimed to evaluate the antibacterial and modulatory potential of α-bisabolol, β-cyclodextrin and α-bisabolol/β-cyclodextrin complex. The minimum inhibitory concentration was determined through the broth microdilution technique using the bacterial strains: Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The drugs norfloxacin, imipenem and gentamicin were used in the tests, and the compounds α-bisabolol and β-cyclodextrin; all the compounds were diluted in DMSO. To obtain the minimum inhibitory concentration (MIC) a serial microdilution of the substances in volumes corresponding to the sub-inhibitory concentration (MIC/8), and microdilution with the antibiotic until the penultimate well were performed...
June 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28615847/larvicidal-potency-of-marine-actinobacteria-isolated-from-mangrove-environment-against-aedes-aegypti-and-anopheles-stephensi
#7
S Balakrishnan, P Santhanam, M Srinivasan
The marine soil samples were collected from different locations of Parangipettai mangrove ecosystem, Vellar estuary, southeast coast of India. Totally 30 different marine actinobacteria were isolated by serial dilution plate technique on starch casein agar medium. The isolated actinobacteria were investigated for their larvicidal activity against Aedes aegypti and Anopheles stephensi mosquitoes. Streptomyces fungicidicus, S. griseus, S. albus, S. alboflavus and S. rochei were identified as potential biocide producers...
June 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28601310/hemoglobin-based-oxygen-carriers-promote-systemic-hyperfibrinolysis-that-is-both-dependent-and-independent-of-plasmin
#8
Alexander P Morton, Ernest E Moore, Hunter B Moore, Eduardo Gonzalez, Michael P Chapman, Erik Peltz, Anirban Banerjee, Christopher Silliman
BACKGROUND: Hyperfibrinolysis plays an integral role in the genesis of trauma-induced coagulopathy. Recent data demonstrate that red blood cell lysis promotes fibrinolysis; however, the mechanism is unclear. Hemoglobin-based oxygen carriers (HBOCs) have been developed for resuscitation and have been associated with coagulopathy. We hypothesize that replacement of whole blood (WB) using an HBOC results in a coagulopathy because of the presence of free hemoglobin. MATERIALS AND METHODS: WB was sampled from healthy donors (n = 6)...
June 1, 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28595928/histopathological-effects-of-cypermethrin-and-bacillus-thuringiensis-var-israelensis-on-midgut-of-chironomus-calligraphus-larvae-diptera-chironomidae
#9
Sabrina Lavarías, Florencia Arrighetti, Augusto Siri
Pesticides are extensively used for the control of agricultural pests and disease vectors, but they also affect non-target organisms. Cypermethrin (CYP) is a synthetic pyrethroid used worldwide. Otherwise, bioinsecticides like Bacillus thuringiensis var. israelensis (Bti) have received great attention as an environmentally benign and desirable alternative. In order to evaluate the toxicity of those pesticides, Chironomus calligraphus was selected due to its high sensitivity to some toxicants. Third and fourth instars larvae were exposed to serial dilutions of CYP and Bti to determine LC50 values...
June 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28591979/investigation-into-the-antimicrobial-action-and-mechanism-of-a-novel-endogenous-peptide-%C3%AE-casein-197-from-human-milk
#10
Yanrong Fu, Chenbo Ji, Xiaohui Chen, Xianwei Cui, Xing Wang, Jie Feng, Yun Li, Rui Qin, Xirong Guo
A novel endogenous peptide cleaved from 197-213 AA of β-casein, named β-casein 197, was identified by tandem mass spectrometry. β-casein 197 constituted a significant proportion of the peptide content in preterm milk. This study investigated the antibacterial effects and mechanisms against common pathogenic bacteria. Six bacterial strains were selected for this study: Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica, Listeria monocytogenes, Klebsiella pneumonia and Bacillus subtilis. After synthesis, serial twofold dilutions of β-casein 197 were added to select for sensitive bacteria...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28588284/a-novel-preventive-strategy-against-hiv-1-infection-combinatorial-use-of-inhibitors-targeting-the-nucleocapsid-and-fusion-proteins
#11
Yu Yang, Jingyu Zhu, Matthew Hassink, Lisa M Miller Jenkins, Yanmin Wan, Daniel H Appella, Jianqing Xu, Ettore Appella, Xiaoyan Zhang
The strategy of simultaneously attacking multiple targets is worthy of exploration in the field of microbicide development to combat HIV-1 sequence diversity and minimize the transmission of resistant variants. A combination of S-acyl-2-mercaptobenzamide thioester-10 (SAMT10), an inhibitor of the HIV-1 nucleocapsid protein (NCp7), and the fusion inhibitor sifuvirtide (SFT) may exert synergistic effects, since SFT can block viral fusion at an early stage of the viral cycle and SAMT10 can disrupt viral particles at a later stage...
June 7, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28588123/a-genoproteomic-approach-to-detect-peptide-markers-of-bacterial-respiratory-pathogens
#12
Honghui Wang, Steven K Drake, Chen Yong, Marjan Gucek, Matthew A Lyes, Avi Z Rosenberg, Erik Soderblom, M Arthur Moseley, John P Dekker, Anthony F Suffredini
BACKGROUND: Rapid identification of respiratory pathogens may facilitate targeted antimicrobial therapy. Direct identification of bacteria in bronchoalveolar lavage (BAL) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry is confounded by interfering substances. We describe a method to identify unique peptide markers of 5 gram-negative bacteria by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for direct pathogen identification in BAL. METHODS: In silico translation and digestion were performed on 14-25 whole genomes representing strains of Acinetobacter baumannii, Moraxella catarrhalis, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Klebsiella pneumoniae...
June 6, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28572040/measuring-influenza-rna-quantity-after-prolonged-storage-or-multiple-freeze-thaw-cycles
#13
Andrea Granados, Astrid Petrich, Allison McGeer, Jonathan B Gubbay
In this study, we aim to determine what effects prolonged storage and repeated freeze/thaw cycles have on the stability of influenza A(H1N1)pdm09 (influenza A/H1N1)RNA. Cloned influenza A/H1N1 RNA transcripts were serially diluted from 8.0-1.0 log10 copies/μl. RT-qPCR was used to measure RNA loss in transcripts stored at -80°C, -20°C, 4°C and 25°C for up to 84days or transcripts undergoing a total of 10 freeze/thaw cycles. Viral load was measured in clinical specimens stored at-80°C for three years (n=89 influenza A RNA extracts; n=35 primary specimens) and in 10 clinical specimens from the 2015/2016 influenza season that underwent 7 freeze/thaw cycles...
May 29, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28570250/effect-of-negative-pressure-wound-therapy-with-instillation-on-bioburden-in-chronically-infected-wounds
#14
Chun Yang, Selena G Goss, Sean Alcantara, Greg Schultz, John C Lantis Ii
INTRODUCTION: Standard negative pressure wound therapy (NPWT) has been shown to help close wounds despite increasing planktonic bioburden. Both planktonic and biofilm critical colonization are associated with delayed wound healing; therefore, reducing microbial colonization is thought to aid wound healing. The use of NPWT with topical antimicrobial irrigation solution has previously shown reduction in quantitative planktonic bioburden when combined with sharp debridement in chronic wounds...
May 25, 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/28569159/fast-and-robust-single-pcr-for-plasmodium-sporozoite-detection-in-mosquitoes-using-the-cytochrome-oxidase-i-gene
#15
Diego F Echeverry, Nicholas A Deason, Victoria Makuru, Jenna Davidson, Honglin Xiao, Julie Niedbalski, Xiaoyu Yu, Jennifer C Stevenson, Hugo Bugoro, Allan Aparaimo, Hedrick Reuben, Robert Cooper, Thomas R Burkot, Tanya L Russell, Frank H Collins, Neil F Lobo
BACKGROUND: Molecular tools for detecting malaria-infected mosquitoes with improved practicality, sensitivity and specificity, and high-throughput are required. A common PCR technique used to detect mosquitoes infected with Plasmodium spp. is a nested PCR assay based on the 18s-rRNA gene. However, this technique has several technical limitations, is laborious and time consuming. METHODS: In this study, a PCR-based on the Plasmodium cytochrome oxidase I (COX-I) gene was compared with the 18s-rRNA nested PCR using serial dilutions (330-0...
May 31, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28566853/bacteriostatic-effect-of-simvastatin-on-selected-oral-streptococci-in-vitro
#16
Eugene J Whitaker, Abdulaziz Alshammari
CONTEXT AND OBJECTIVE: Simvastatin is a widely used cholesterol-lowering drug, which has been found to have a number of pleiotropic effects. The aim of this study was to evaluate the antimicrobial effectiveness of simvastatin against selected oral streptococci as determined by the minimum inhibitory concentration (MIC). METHODS: Streptococcus mutans, Streptococcus sanguis, Streptococcus anginosus, and Streptococcus salivarius were the test microorganisms. The serial dilution method was used to determine the MIC of simvastatin against these organisms...
January 2017: Contemporary Clinical Dentistry
https://www.readbyqxmd.com/read/28561638/mitigation-of-salmonella-on-pet-food-kibbles-by-using-liquid-and-powdered-3-hydroxy-3-methylbutyric-acid
#17
Anne R Huss, John C Fuller, William Centrella, Douglas L Marshall, Aiswariya Deliephan, Cassandra K Jones
In recent years, several pet food recalls have been attributed to Salmonella contamination. In addition to the negative impacts on animal health, Salmonella-contaminated pet foods have been linked to infection in humans. With that in mind, the U.S. Food and Drug Administration has set forth a zero-tolerance policy for Salmonella in pet foods. Typically, pet foods are extruded or processed at high temperatures that are sufficient to reduce pathogenic bacteria. However, the possibility for postextrusion contamination still exists...
May 31, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28558918/general-phytoplasma-detection-by-a-q-pcr-method-using-mycoplasma-primers
#18
Eleonora Satta, Irene M Nanni, Nicoletta Contaldo, Marina Collina, José B Poveda, Ana S Ramírez, Assunta Bertaccini
Phytoplasmas and mycoplasmas are bacteria belonging to the class Mollicutes. In this study, a fine tuning of quantitative polymerase chain reaction (qPCR) with a universal mycoplasma primer pair (GPO3F/MGSO) targeting the 16S rRNA gene was carried out on phytoplasmas. The dissociation curves of DNAs from Catharanthus roseus phytoplasma-infected micropropagated shoots and from phytoplasma field-infected plant samples showed a single peak at 82.5 °C (±0.5) specifically detecting phytoplasmas belonging to several ribosomal groups...
May 27, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28558683/inhibition-of-biofilm-formation-in-mycobacterium-smegmatis-by-parinari-curatellifolia-leaf-extracts
#19
Benjamin Bhunu, Ruvimbo Mautsa, Stanley Mukanganyama
BACKGROUND: Tuberculosis (TB) is a serious public health problem worldwide. Mycobacterium tuberculosis (M. tuberculosis) grows as drug tolerant pellicles. Agents that inhibit biofilm formation in M. tuberculosis have the potential to reduce the disease treatment period and improve the quality of tuberculosis chemotherapy. Parinari curatellifolia (P. curatellifolia) leaf extracts are claimed to treat symptoms similar to tuberculosis in ethnomedicinal practices. Mycobacterium smegmatis (M...
May 30, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28549942/tissue-specific-expression-profile-of-some-immune-related-genes-in-labeo-rohita-to-edwardsiella-tarda-infection
#20
Sajal Kole, Deepika Anand, Rupam Sharma, Gayatri Tripathi, M Makesh, K V Rajendran, Megha Kadam Bedekar
Rohu (Labeo rohita), an Indian Major Carp (IMC) is an economically important aquaculture species in India. Inspite of the technological advances, infectious diseases caused by viruses, bacteria and parasites have been a major limiting factor in the development and profitability of fish farms. At present, information regarding the immune status of the Indian major carps is limited. This lack of knowledge is a major impediment for establishment of effective preventive measures against broad spectrum of infectious agents...
May 23, 2017: Fish & Shellfish Immunology
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