keyword
MENU ▼
Read by QxMD icon Read
search

XTT

keyword
https://www.readbyqxmd.com/read/28925481/functional-impact-of-galectin-3-and-trail-expression-in-breast-cancer-cells
#1
X-H Liu, C-X Deng, P-C Hu, Y Wang, Y-H Dong
OBJECTIVE: To examine the expression of Galectin-3 and TRAIL in breast cancer tissue and their effects on the proliferation and apoptosis of breast cancer cells. PATIENTS AND METHODS: Breast cancer and normal adjacent tissue were collected from 120 patients pathologically diagnosed with breast cancer who underwent a modified radical mastectomy. SP method of immunohistochemistry was used to detect the expression levels of Galectin-3 and TRAIL in breast cancer tissues and normal adjacent tissues...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28919715/in-vitro-inhibitory-activities-of-magnolol-against-candida-spp
#2
Peiru Zhou, Jingya Fu, Hong Hua, Xiaosong Liu
Candida spp. cause various infections involving the skin, mucosa, deep tissues, and even life-threatening candidemia. They are regarded as an important pathogen of nosocomial bloodstream infection, with a high mortality rate. As a result of prolonged exposure to azoles, the therapeutic failure associated with azoles resistance has become a serious challenge in clinical situations. Therefore, novel, alternative antifungals are required urgently. In the present study, the CLSI M-27A broth microdilution method and the 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay were used to evaluate the antifungal effects of magnolol against various standard Candida strains in planktonic mode and biofilm formation, respectively...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28919583/polyclonal-antibody-against-different-extracellular-subdomains-of-her2-induces-tumor-growth-inhibition-in-vitro
#3
Reza Hosseini Ghatar, Tahereh Soltantoyeh, Motahareh Bahadori, Jalal Khoshnoodi, Forough Golsaz Shirazi, Mahmood Jeddi Tehrani, Mohammad Mehdi Amiri, Fazel Shokri
BACKGROUND: Human epidermal growth factor receptor 2 (HER2) has a crucial role in several malignancies. The extracellular domain of HER2 (HER2-ECD) has been extensively employed as an important target in passive and active immunotherapy. Isolated recombinant prokaryotic HER2-ECD subdomains were previously found to be ineffective in inducing anti-tumor antibody response. OBJECTIVE: To employ recombinant eukaryotic HER2-ECD subdomains to raise anti-HER2 antibodies and determine their anti-tumor activity in vitro...
September 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28901907/isolates-of-lactobacillus-plantarum-and-l-reuteri-display-greater-antiproliferative-and-antipathogenic-activity-than-other-lactobacillus-isolates
#4
Meysam Hasannejad Bibalan, Morteza Eshaghi, Mahdi Rohani, Maryam Esghaei, Davood Darban-Sarokhalil, Mohammad R Pourshafie, Malihe Talebi
PURPOSE: Lactic acid bacteria (LAB) have been associated with many beneficial effects in human digestive physiology. The aim of this study was to evaluate such effect, including attachment, antiproliferation and anti-pathogenic/antibacterial/antimicrobial properties of LAB isolated from healthy humans. METHODOLOGY: Thirteen isolates, obtained from fecal samples of healthy individuals, were identified by phenotypic and molecular methods. Human colon adenocarcinoma cell line HT-29 and the cell proliferation kit II (XTT) assay were used for examination of the Lactobacillus adherence and antiproliferative activity, respectively...
September 13, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28900419/enhanced-killing-and-antibiofilm-activity-of-encapsulated-cinnamaldehyde-against-candida-albicans
#5
Shahper N Khan, Shakir Khan, Jawed Iqbal, Rosina Khan, Asad U Khan
Candida sp. impelled opportunistic infection in immune-compromised patients ensuing from asymptomatic colonization to pathogenic forms. Moreover, slow spread of Candida species inducing refractory mucosal and invasive infections brings acute resistance to antifungal drugs. Hence, here we probed the effect of encapsulated preparation of cinnamaldehyde (CNMA) in multilamellar liposomes (ML) against Candida albicans. The efficacy of ML-CNMA against Candida biofilm was assessed by scanning electron microscopy, transmission electron microscopy, as well as light microscopy and its percent inhibition, was determined by XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] and crystal violet assay...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28893370/inhibitory-efficacy-of-geraniol-on-biofilm-formation-and-development-of-adaptive-resistance-in-staphylococcus-epidermidis-rp62a
#6
Arunachalam Kannappan, Murugesan Sivaranjani, Ramanathan Srinivasan, Janarthanam Rathna, Shunmugiah Karutha Pandian, Arumugam Veera Ravi
PURPOSE: The current study has been designed to delineate the efficacy of geraniol (GE) on biofilm formation in Staphylococcus epidermidis as well as the effect of subinhibitory concentrations of GE on the development of adaptive resistance. METHODOLOGY: Biofilm biomass quantification assay was performed to evaluate the antibiofilm activity of GE against S. epidermidis. Microscopic observation of biofilms and extracellular polymeric substance (EPS), slime and cell surface hydrophobicity (CSH) production were also studied to support the antibiofilm potential of GE...
September 12, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28869462/applying-xtt-wst-1-and-wst-8-to%C3%A2-human-chondrocytes-a-comparison-of%C3%A2-membrane-impermeable-tetrazolium-salts-in-2d-and-3d-cultures
#7
Anne-Helen Lutter, Jenny Scholka, Heiko Richter, Ursula Anderer
BACKGROUND: Tetrazolium-based assays are optimized to assess proliferation/toxicity of monolayer or suspension cells in microtiter plates. With regard to tissue engineering and regenerative medicine the need for in vivo like 3D microtissues has an increasing relevance. Applying tetrazolium-based assays to 3D culture systems is technically more challenging. The composed microenvironment may influence the assay standards, e.g. equal distribution of tetrazolium. OBJECTIVE: Evaluation of membrane-impermeable tetrazolium salt-based assays with regard to spheroid culture (3D) of human chondrocytes...
August 28, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28844680/korean-red-ginseng-panax-ginseng-inhibits-obesity-and-improves-lipid-metabolism-in-high-fat-diet-fed-castrated-mice
#8
Soon Shik Shin, Michung Yoon
ETHNOPHARMACOLOGICAL RELEVANCE: Korean red ginseng (Panax ginseng C.A. Meyer, Araliaceae) has been historically used as a traditional drug for the prevention and treatment of most ageing-related diseases, such as obesity, dyslipidemia, diabetes, and cardiovascular disease. Elderly men with testosterone deficiency are strongly associated with many of the aforementioned metabolic diseases. The aim of this study was to determine the effects of ginseng on obesity and lipid metabolism in a mouse model of testosterone deficiency (castrated C57BL/6J mice)...
August 24, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28837052/bismuth-subsalicylate-nanoparticles-with-anaerobic-antibacterial-activity-for-dental-applications
#9
Alejandro Luis Vega-Jiménez, Argelia Almaguer-Flores, Mariela Flores-Castañeda, Enrique Camps, Marisela Uribe-Ramirez, O Gamaliel Aztatzi-Aguilar, Andrea De Vizcaya-Ruiz
In recent years, nanomaterials have been used in the medical-dental field as new alternative antimicrobial agents. Bismuth subsalicylate (BSS) has been used as an antimicrobial agent, but the effect of BSS in the form of nanoparticles (BSS-nano) as a potential antimicrobial agent has not been tested, in specific against bacteria responsible for periodontal disease. The aim of this study was to evaluate the antibacterial effect of BSS-nano against oral anaerobic bacteria and to assess the safety of BSS-nano by evaluating their cytotoxicity in human gingival fibroblast (HGF-1) cells...
August 24, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28835169/computational-design-of-phe-tyr-dipeptide-and-preparation-characterization-cytotoxicity-studies-of-phe-tyr-dipeptide-loaded-plga-nanoparticles-for-the-treatment-of-hypertension
#10
Serda Kecel-Gündüz, Yasemin Budama-Kilinc, Rabia Cakir Koc, Yagmur Kökcü, Bilge Bicak, Bahar Aslan, Aysen E Özel
Phe-Tyr dipeptide which was investigated in Wakame food with greatest ACE-inhibitory activity is used as a pharmaceutical drug for the treatment of hypertension, cardiovascular diseases and diabetic nephropathy. To improve the bioavailability of Phe-Tyr, a delivery system based on poly (lactic-co-glycolic acid) (PLGA) nanoparticles loaded with Phe-Tyr (Phe-Tyr-PLGA NPs) for treating hypertension and cardiovascular diseases was prepared in this study. In the experiments, poly(lactic-co-glycolic acid) (PLGA) and Phe-Tyr dipeptide-loaded PLGA nanoparticles were prepared using the double emulsion (w/o/w) method...
August 23, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28834275/synthesis-biological-evaluation-and-molecular-docking-studies-of-novel-4-arylpyridin-1-4h-yl-benzoic-acid-derivatives-as-anti-hiv-1-agents
#11
Saghi Sepehri, Sepehr Soleymani, Rezvan Zabihollahi, Mohammad R Aghasadeghi, Mehdi Sadat, Lotfollah Saghaie, Afshin Fassihi
The structural similarities between N1 substituted 1,4-dihydropyridines and the known gp41 inhibitors, NB-2 and NB-64, were considered in the current research for the design of some novel anti-HIV-1 agents. A series of novel 4-arylpyridin-1(4H)-yl) benzoic acid derivatives were synthesized and after a comprehensive structural elucidation were screened for in vitro anti-HIV-1 activity. Most of the tested compounds displayed moderate to good inhibitory activity against HIV-1 growth and were evaluated for in vitro cytotoxic activity using XTT assay at the concentration of 100 μM...
August 21, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28821108/modified-bacterial-cellulose-based-self-healable-polyeloctrolyte-film-for-wound-dressing-application
#12
Moumita Khamrai, Sovan Lal Banerjee, Patit P Kundu
In this investigation, we prepare a self-healable polyelectrolyte film via crosslinking the cationically charged chitosan (Cts) with anionically modified bacterial cellulose (BC), which is a green source of nano-filler. This polyelectrolyte film is able to show dynamic self-healing activity at physiological pH condition via adapting ionic interaction, a state of non-covalent bond. BC was prepared using Glucanoacetobacter xylinus (MTCC7795) bacteria and after that its surface was modified with anionic poly(acrylic acid) using "grafting from" technique...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28807849/danshen-improves-survival-of-patients-with-colon-cancer-and-dihydroisotanshinone-i-inhibit-the-proliferation-of-colon-cancer-cells-via-apoptosis-and-skp2-signaling-pathway
#13
Yin-Yin Lin, I-Yun Lee, Wen-Shih Huang, Yu-Shin Lin, Feng-Che Kuan, Li-Hsin Shu, Yu-Ching Cheng, Yao-Hsu Yang, Ching-Yuan Wu
ETHNOPHARMACOLOGICAL RELEVANCE: Danshen (Salvia miltiorrhiza Bunge) is widely used in traditional Chinese medicine. However, it's definite clinical effect and mechanism on colon carcinoma is unclear. AIM OF THE STUDY: To test the hypothesis that the protective effect of danshen on colon cancer and discover the bioactive compounds through in vitro study. MATERIALS AND METHODS: We conducted a nationwide cohort study by using population-based data from the Taiwan National Health Insurance Research Database (NHIRD)...
August 12, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28803881/cell-specific-and-dose-dependent-effects-of-pahs-on-proliferation-cell-cycle-and-apoptosis-protein-expression-and-hormone-secretion-by-placental-cell-lines
#14
Eliza Drwal, Agnieszka Rak, Adam Grochowalski, Tomasz Milewicz, Ewa Lucja Gregoraszczuk
In the preset study we measured the concentrations of 16 priority PAHs in maternal blood and placental tissue by using the GC-MS/MS system, and investigated the effects of selected PAHs (naphthalene, anthracene, phenanthrene, pyrene) and mixtures on BeWo and JEG-3 human placental cell line proliferation (Alamar Blue), cytotoxicity (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (XTT), lactate dehydrogenase (LDH), acid phosphatase (AP), endocrine activity (progesterone and estradiol secretion) and apoptosis (cyclin A1, cyclin D2, cdk 2, cdk 4, Bcl-xl, Bax, and caspase-3 protein expression)...
August 10, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28794934/activity-of-chitosan-lysozyme-nanoparticles-on-the-growth-membrane-integrity-and-%C3%AE-1-3-glucanase-production-by-aspergillus-parasiticus
#15
Cynthia Nazareth Hernández-Téllez, Francisco Julián Rodríguez-Córdova, Ema Carina Rosas-Burgos, Mario Onofre Cortez-Rocha, Armando Burgos-Hernández, Jaime Lizardi-Mendoza, Wilfrido Torres-Arreola, Aarón Martínez-Higuera, Maribel Plascencia-Jatomea
Synthesis of nanocomposites from antimicrobial biopolymers such as chitosan (CS) and lysozyme (LZ) is an important and promising area in bionanotechnology. Chitosan-lysozyme (CS-LZ) nanoparticles (NPs) were prepared by the nanoprecipitation method, using commercial chitosan of 153 kDa. TEM and dynamic light scattering (DLS) analysis were carried out to evaluate the morphology, size, dispersion, and Z potential. Association efficiency of lysozyme was determined using Coomassie blue assay. The antifungal activity of NPs against Aspergillus parasiticus was evaluated through cell viability (XTT), germination and morphometry of spores, and reducing sugars production; the effects on membrane integrity and cell wall were also analyzed...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28772791/heat-polymerized-resin-containing-dimethylaminododecyl-methacrylate-inhibits-candida-albicans-biofilm
#16
Hui Chen, Qi Han, Xuedong Zhou, Keke Zhang, Suping Wang, Hockin H K Xu, Michael D Weir, Mingye Feng, Mingyun Li, Xian Peng, Biao Ren, Lei Cheng
The prevalence of stomatitis, especially caused by Candida albicans, has highlighted the need of new antifungal denture materials. This study aimed to develop an antifungal heat-curing resin containing quaternary ammonium monomer (dimethylaminododecyl methacrylate, DMADDM), and evaluate its physical performance and antifungal properties. The discs were prepared by incorporating DMADDM into the polymer liquid of a methyl methacrylate-based, heat-polymerizing resin at 0% (control), 5%, 10%, and 20% (w/w). Flexure strength, bond quality, surface charge density, and surface roughness were measured to evaluate the physical properties of resin...
April 20, 2017: Materials
https://www.readbyqxmd.com/read/28769026/chemical-composition-antioxidant-anti-inflammatory-and-antiproliferative-activities-of-the-plant-lebanese-crataegus-azarolus-l
#17
Hany Kallassy, Mohammad Fayyad-Kazan, Rawan Makki, Yolla El-Makhour, Eva Hamade, Hasan Rammal, David Y Leger, Vincent Sol, Hussein Fayyad-Kazan, Bertrand Liagre, Bassam Badran
BACKGROUND In the present study, phytochemical screening, antioxidant, anti-inflammatory, and antiproliferative capacities of 3 extracts from leaves of Lebanese Crataegus azarolus L. were evaluated. MATERIAL AND METHODS Fresh leaves were dissolved in 3 different solvents: distilled water, ethanol, and methanol. The chemical composition was determined using high-performance liquid chromatography (HPLC) and the content of essential oil of this plant was examined by gas chromatography (GC) coupled with mass spectrometry (MS)...
August 3, 2017: Medical Science Monitor Basic Research
https://www.readbyqxmd.com/read/28767065/do-multiwell-plate-high-throughput-assays-measure-loss-of-cell-viability-following-exposure-to-genotoxic-agents
#18
Razmik Mirzayans, Bonnie Andrais, David Murray
Cell-based assays in multiwell plates are widely used for radiosensitivity and chemosensitivity assessment with different mammalian cell types. Despite their relative ease of performance, such assays lack specificity as they do not distinguish between the cytostatic (reversible/sustained growth arrest) and cytotoxic (loss of viability) effects of genotoxic agents. We recently reported studies with solid tumor-derived cell lines demonstrating that radiosensitivity as measured by multiwell plate colorimetric (e...
August 2, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28764751/the-in-vitro-effects-of-interferon-gamma-alone-or-in-combination-with-amphotericin-b-tested-against-the-pathogenic-fungi-candida-albicans-and-aspergillus-fumigatus
#19
Moufid El-Khoury, Rogine Ligot, Simon Mahoney, Colin M Stack, Gabriel G Perrone, C Oliver Morton
OBJECTIVE: Recent studies into the antifungal activity of NK-cells against the Aspergillus fumigatus have presented differing accounts on their mode of antifungal activity. One of these mechanisms proposed that NK-cells may kill the fungus via the direct effects of exposure to Interferon gamma (IFN-γ). RESULTS: In this study we investigated the direct antifungal effects of recombinant human IFN-γ against a range of pathogenic fungi by measuring cellular damage using an XTT-based assay and cell viability through plate counts...
August 1, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28763317/rocuronium-is-more-hepatotoxic-than-succinylcholine-in-vitro
#20
Martin Sauer, Ines Piel, Cristof Haubner, Georg Richter, Miriam Mann, Gabriele Nöldge-Schomburg, Thomas Mencke
BACKGROUND: The development of liver failure is a major problem in critically ill patients. The hepatotoxicity of many drugs, as one important reason for liver failure, is poorly screened for in human models. Rocuronium and succinylcholine are neuromuscular blocking agents used for tracheal intubation and for rapid-sequence induction. OBJECTIVE: We used an in-vitro test with a permanent cell line and compared rocuronium and succinylcholine for hepatotoxicity. DESIGN: In-vitro study...
September 2017: European Journal of Anaesthesiology
keyword
keyword
96953
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"