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https://www.readbyqxmd.com/read/28711338/in-vitro-cytocompatibility-of-dental-resin-monomers-on-osteoblast-like-cells
#1
Dominik Kraus, Markus Wolfgarten, Norbert Enkling, Ernst-Heinrich Helfgen, Matthias Frentzen, Rainer Probstmeier, Jochen Winter, Helmut Stark
OBJECTIVES: Dental resin-based materials are widely used in modern dentistry. Especially, resin cements enjoy great popularity and are utilized in many applications. Nevertheless, monomers could be released from the resinous matrix, thus interact with surrounding tissues, cause adverse biological reactions and may lead in cases of implant retained restorations to peri-implant bone destruction. Hence, we performed an in-vitro study to determine cytotoxicity of resin monomers on osteoblast-like cells...
July 12, 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28710011/antibacterial-activity-of-food-grade-chitosan-against-vibrio-parahaemolyticus-biofilms
#2
Ting Xie, Zhenlin Liao, Huan Lei, Xiang Fang, Jie Wang, Qingping Zhong
Biofilm is a community composed of microbes and the extracellular polymeric substances. This special architecture poses a significant public health risk as it increases the fitness of bacteria in harsh conditions and renders bacterial resistance to antimicrobial agents and cleaning. In this study, we investigated the inhibition and eradication effects of chitosan on the biofilm of Vibrio parahaemolyticus, an important food-borne pathogen. The crystal violet staining, [2, 3-bis (2-methoxy-4-nitro-5- sulfophenyl)-2H-tetrazolium-5-carboxanilide] (XTT) reduction method, phenol-sulfuric acid method, fluorescence microscope and confocal laser scanning microscope (CLSM) observation were conducted...
July 11, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28709945/punicalagin-a-polyphenol-from-pomegranate-fruit-induces-growth-inhibition-and-apoptosis-in-human-pc-3-and-lncap-cells
#3
Oluwatosin Adaramoye, Bettina Erguen, Bianca Nitzsche, Michael Höpfner, Klaus Jung, Anja Rabien
Prostate cancer (PCa) is an international health problem and search for its effective treatment is in progress. Punicalagin (PN), polyphenol from pomegranate fruit, is known to exhibit potent anticancer activity in lung, breast and cervical cells. However, there is paucity of information on its effect in PCa. This study evaluated anti-proliferative effects of PN and its effects on extrinsic pathway of apoptosis in PCa cells, and angiogenesis in chicken chorioallantoic membrane (CAM). Antioxidant activities of PN were determined by 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging and inhibition of lipid peroxidation (LPO) methods...
July 11, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28699308/phospholipid-capped-mesoporous-nanoparticles-for-targeted-high-intensity-focused-ultrasound-ablation
#4
Adem Yildirim, Rajarshi Chattaraj, Nicholas T Blum, Dennis Shi, Kaushlendra Kumar, Andrew P Goodwin
The mechanical effects of cavitation can be effective for therapy but difficult to control, thus potentially leading to off-target side effects in patients. While administration of ultrasound active agents such as fluorocarbon microbubbles and nanodroplets can locally enhance the effects of high intensity focused ultrasound (HIFU), it has been challenging to prepare ultrasound active agents that are small and stable enough to accumulate in tumors and internalize into cancer cells. Here, this paper reports the synthesis of 100 nm nanoparticle ultrasound agents based on phospholipid-coated, mesoporous, hydrophobically functionalized silica nanoparticles that can internalize into cancer cells and remain acoustically active...
July 12, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28696482/cytotoxicity-and-genotoxicity-of-stilbene-derivatives-in-cho-k1-and-hepg2-cell-lines
#5
Cassia Suemi Mizuno, Winnifred Ampomaah, Fernanda Ribeiro Mendonça, Gabriela Carvalho Andrade, Ariel Maria Nazaré da Silva, Mirian Oliveira Goulart, Raquel Alves Dos Santos
The cytotoxicity and genotoxicity of the stilbenes (E)-methyl-4-(3-5-dimethoxystyryl)benzoate (ester), (E)-4-(3-5-dimethoxystyryl)aniline (amino), (Z)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (cis-TMS) and (E)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (trans-TMS) were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses...
July 10, 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28681217/how-calcite-and-modified-hydroxyapatite-influence-physicochemical-properties-and-cytocompatibility-of-alpha-tcp-based-bone-cements
#6
Aneta Zima, Joanna Czechowska, Dominika Siek, Radosław Olkowski, Magdalena Noga, Małgorzata Lewandowska-Szumieł, Anna Ślósarczyk
Nowadays successful regeneration of damaged bone tissue is a major problem of the reconstructive medicine and tissue engineering. Recently a great deal of attention has been focused on calcium phosphate cements (CPCs) as the effective bone fillers. Despite a number of studies regarding CPCs, only a few compare the physicochemical and biological properties of α-TCP based materials of various phase compositions. In our study we compared the effect of several components (calcite, hydroxyapatite doped with Mg(2+), CO3(2-) or Ag(+) ions, alginate, chitosan and methylcellulose) on the physicochemical and biological properties of α-TCP-based bone cements...
August 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28674704/antibacterial-orthodontic-adhesive-incorporating-polyethyleneimine-nanoparticles
#7
Nathan Zaltsman, Dana Kesler Shvero, David Polak, Ervin I Weiss, Nurit Beyth
PURPOSE: To evaluate the antibacterial, mechanical and biocompatibility characteristics of an orthodontic adhesive that contains quaternary ammonium polyethyleneimine (QPEI) nanoparticles. MATERIALS AND METHODS: QPEI nanoparticles were added to an orthodontic adhesive at 0%, 1% and 1.5% wt/wt. Antibacterial activity was tested after aging for 14 days using the direct contact test (DCT). The degree of monomer conversion (DC) was measured using Fourier transform infrared (FTIR) spectroscopy...
2017: Oral Health & Preventive Dentistry
https://www.readbyqxmd.com/read/28665155/effects-of-high-power-pulsed-nd-yag-laser-irradiation-on-the-release-of-transforming-growth-factor-beta-tgf-%C3%AE-and-vascular-endothelial-growth-factor-vegf-from-human-gingival-fibroblasts
#8
Mehmet Sağlam, Serhat Köseoğlu, Tuğba Pekbağrıyanık, Levent Savran, Şükrü Enhoş
The purpose of this study was to investigate the effect of different high power energy settings of a Nd:YAG laser (1064-nm) on cell viability of human gingival fibroblasts and release of transforming growth factor-beta (TGF-β) and vascular endothelial growth factor (VEGF) on these cells. Gingival fibroblasts (GF) were isolated from human gingival connective tissue during crown lengthening procedure. GF were irradiated with different laser parameters as follows; group 1: 1 W (100 mj, 10 Hz) 10 s, group 2: 1,5 W (150 mj, 10 Hz) 10 s, group 3: 2 W (200mj, 10 Hz) 10 s, group 4: 1 W (100 mj, 10 Hz) 20 s, group 5: 1,5 W (150 mj, 10 Hz) 20 s, group 6: 2 W (200mj, 10 Hz) 20 s...
June 30, 2017: Journal of Cosmetic and Laser Therapy: Official Publication of the European Society for Laser Dermatology
https://www.readbyqxmd.com/read/28650789/effects-of-cola-flavored-beverages-and-caffeine-on-streptococcus-mutans-biofilm-formation-and-metabolic-activity
#9
Roger P Dotsey, Elizabeth A S Moser, George J Eckert, Richard L Gregory
OBJECTIVE: To examine the effects of cola-flavored beverages and caffeine on growth and metabolism of Streptococcus mutans biofilm. This study was designed to determine if carbonated beverages or caffeine can increase S. mutans growth and biofilm formation and metabolic activity in vitro, potentially leading to increased S. mutans-associated cariogenicity in children that consume them. STUDY DESIGN: Six different cola-flavored products, plus pure caffeine, and pure high fructose corn syrup (HFCS), at different concentrations similar to those in the beverages were tested...
2017: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28645774/evaluation-of-selected-indian-medicinal-plants-for-antagonistic-potential-against-malassezia-spp-and-the-synergistic-effect-of-embelin-in-combination-with-ketoconazole
#10
Chandran Sivasankar, Subramanian Gayathri, James Prabhanand Bhaskar, Venkat Krishnan, Shunmugiah Karutha Pandian
The genus Malassezia comprises of extremely lipophilic yeasts secreting lipases as a vital factor for survival. They are emerging as opportunistic pathogens in medical microbiology and dermatology by causing recurring and recalcitrant infection. Combinatorial therapy is a constructive way to combat infectious diseases. In that prospect, totally 16 Indian medicinal plants were screened, among which a maximum degree of antimicrobial activity was ascertained in Embelia ribes. Subsequently embelin was identified as the bioactive principle with antagonistic potential by comparative antimicrobial assay and FTIR analysis...
June 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28642925/the-effect-of-gelatinase-production-of-enterococcus-faecalis-on-adhesion-to-dentin-after-irrigation-with-various-endodontic-irrigants
#11
Mehmet Burak Guneser, Ayce Unverdi Eldeniz
Objectives: The aim of this study was to evaluate whether the gelatinase production ability of Enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. Materials and methods: Standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (NaOCl), group 2: 2% chlorhexidine (CHX), group 3: NaOCl + Saline + CHX, group 4: NaOCl + EDTA + NaOCl, group 5: QMix. After incubation of dentin discs with irrigants, each group was divided into two subgroups (n = 10) according to the bacterial strains used; a gelatinase-producing and a gelatinase-deficient strain of E...
December 2016: Acta Biomaterialia Odontologica Scandinavica
https://www.readbyqxmd.com/read/28637488/a-high-throughput-colorimetric-assay-for-detection-of-schistosoma-mansoni-viability-based-on-the-tetrazolium-salt-xtt
#12
Pedro Henrique Nascimento Aguiar, Núbia Monteiro Gonçalves Soares Fernandes, Carlos Leomar Zani, Marina Moraes Mourão
BACKGROUND: Schistosoma mansoni is a trematode parasite that causes schistosomiasis, one of the most prevalent neglected tropical diseases, leading to the loss of 2.6 million disability-adjusted life years. Praziquantel is the only drug available, and new drugs are required. The most common strategy in schistosomiasis drug discovery is the use of the schistosomula larval-stage for a pre-screen in drug sensitivity assays. However, assessing schistosomula viability by microscopy has always been a limitation to the throughput of such assays...
June 21, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28630386/low-17%C3%AE-estradiol-levels-are-better-inducers-of-regulatory-conditioned-t-cells-in-vitro
#13
Ramina Fatemi, Ebrahim Mirzadegan, Zohreh Vahedian, Amir Hassan Zarnani, Mahmood Jeddi-Tehrani, Farah Idali
BACKGROUND: 17β-estradiol (E2) has been known to modulate immune response. Recent studies indicate that E2 at pregnancy level plays a role in regulating T cell response. OBJECTIVE: To investigate the optimum dose of E2 (from 10-9 to 10-7 M) in mediating the generation of regulatory T cells (Tregs), using naive human CD4+ T cells from healthy women. METHODS: Naive peripheral T cells were purified and conditioned with soluble anti-CD28 in anti-CD3-coated plates in the presence or absence of E2...
June 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28630381/antibody-response-to-human-extracellular-her2-subdomain-proteins-in-mice
#14
Fateme Sadri-Ardalani, Moslem Ahmadi, Azam Hemmati, Shaghayegh Emami, Samira Farid, Mohammad Mehdi Amiri, Mahmood Jeddi-Tehrani, Mahdi Shabani, Fazel Shokri
BACKGROUND: In addition to passive immunotherapy using anti-HER2 monoclonal antibodies, active immunotherapy via HER2 targeting is an interesting approach to inducing specific anti-tumor immune responses. We have recently reported the immunogenicity of HER2 subdomains following DNA immunization and HER2 protein boosting. In the present study, we evaluated the immunogenicity of different HER2 extracellular subdomains for the induction of anti-HER2 antibody response in BALB/c mice. OBJECTIVE: To investigate and characterize antibody responses to human recombinant proteins of HER2 extracellular subdomains in immunized mice...
June 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28621579/optimization-of-antimicrobial-photodynamic-therapy-in-biofilms-by-inhibiting-efflux-pump
#15
Tatiana Maria Starck Fogaça de Aguiar Coletti, Laura Marise de Freitas, Ana Marisa Fusco Almeida, Carla Raquel Fontana
BACKGROUND: One of the main mechanisms of microbial resistance is given by efflux pumps, which reduce the effectiveness of antimicrobials by decreasing their intracellular concentration. OBJECTIVE AND METHODS: Considering that efflux pump inhibitors are promising adjuvant molecules for antibiotics in infections, in this study, using XTT test and colony forming unit (CFU) counting, we evaluated the association between the pump inhibitor verapamil (VP) and the antimicrobial photodynamic therapy (aPDT) mediated by methylene blue (MB) in biofilms of Escherichia coli and Staphylococcus aureus to optimize the bacterial reduction...
July 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28607012/potent-in-vitro-synergism-of-fluconazole-and-osthole-against-fluconazole-resistant-candida-albicans
#16
De-Dong Li, Dong Chai, Xiao-Wen Huang, Shao-Xing Guan, Jiang Du, Hao-Yu Zhang, Yan Sun, Yuan-Ying Jiang
Osthole is a natural coumarin that exhibits wide biological and pharmacological activities such as neuroprotective, osteogenic, immunomodulation, anti-tumor and anti-inflammatory effects. In this study, we investigated the antifungal effects of osthole in vitro Checkerboard microdilution assay showed that osthole has significant synergistic effect with fluconazole against fluconazole-resistant Candida albicans Similar results was obtained from growth curve assay. Meanwhile, XTT reduction assay demonstrated the synergism of fluconazole and osthole against C...
June 12, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28603699/crispr-interference-crispri-inhibition-of-luxs-gene-expression-in-e-coli-an-approach-to-inhibit-biofilm
#17
Azna Zuberi, Lama Misba, Asad U Khan
Biofilm is a sessile bacterial accretion embedded in self-producing matrix. It is the root cause of about 80% microbial infections in human. Among them, E. coli biofilms are most prevalent in medical devices associated nosocomial infections. The objective of this study was to inhibit biofilm formation by targeting gene involved in quorum sensing, one of the main mechanisms of biofilm formation. Hence we have introduced the CRISPRi, first time to target luxS gene. luxS is a synthase, involved in the synthesis of Autoinducer-2(AI-2), which in turn guides the initial stage of biofilm formation...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28599387/antioxidant-and-antiproliferative-potentials-of-methanol-extract-of-xylopia-aethiopica-dunal-a-rich-in-pc-3-and-lncap-cells
#18
Oluwatosin Adekunle Adaramoye, Bettina Erguen, Bianca Nitzsche, Michael Höpfner, Klaus Jung, Anja Rabien
BACKGROUND: Our previous studies showed that fruit methanol extract from Xylopia aethiopica (MEXA) exhibited antiproliferative activity in human cervical cancer cells via the induction of apoptosis. The present study was designed to assess the antiproliferative, antiangiogenic and antioxidant effects of MEXA on prostate cancer (PCa) cells (PC-3 and LNCaP). METHODS: PC-3 and LNCaP cells were cultured and treated with MEXA (10, 50 and 100 μg/mL). The sodium 3'-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene sulfonic acid hydrate (XTT) and lactate dehydrogenase (LDH) assays were used to evaluate cell viability and cytotoxicity, respectively...
July 26, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/28587857/comparison-of-the-xtt-and-resazurin-assays-for-quantification-of-the-metabolic-activity-of-staphylococcus-aureus-biofilm
#19
Beatriz Alonso, Raquel Cruces, Adrián Pérez, Carlos Sánchez-Carrillo, María Guembe
We assessed whether resazurin was as efficient as XTT in the measurement of the metabolic activity of 209 clinical Staphylococcus aureus biofilm using an vitro model comparing the percentage of formazan and resorufin. The overall categorical agreement was 61.2% (r=0.024), which means that resazurin can not substitute XTT.
June 3, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28580314/continuous-or-transient-high-level-of-glucose-exposure-differentially-increases-coronary-artery-endothelial-cell-proliferation-and-human-colon-cancer-cell-proliferation
#20
Yoko Shimoda, Yuko Tagaya, Tsugumichi Saito, Eijiro Yamada, Aya Osaki, Yasuyo Nakajima, Atsushi Ozawa, Tetsurou Satoh, Junichi Okada, Shuichi Okada, Masanobu Yamada
We studied effect of high glucose levels on coronary artery endothelial cell proliferation and human colon cancer cell proliferation. To examine the long-term effect of glucose exposure on cell growth, cells were cultured for 14 days in the absence or presence of 183 mg/dL D-glucose addition in the culture medium. Short effect of elevated glucose levels was examined by addition of 183 mg/dL D-glucose addition in the culture medium for just one hour per day followed by changing the culture to standard medium (5...
2017: Cell Journal
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