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https://www.readbyqxmd.com/read/27914077/making-fluorescent-streptococci-and-enterococci-for-live-imaging
#1
Sarah Shabayek, Barbara Spellerberg
Since the discovery of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, outstanding fluorescent labeling tools with numerous applications in vastly different areas of life sciences have been developed. To optimize GFP for diverse life science applications, a large variety of GFP derivatives with different environmental characteristics have been generated by mutagenesis. The enhanced green fluorescent protein (EGFP) is a well-known GFP derivative with highly increased fluorescence intensity compared to the GFP wild-type molecule...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27914045/achievements-and-challenges-in-computational-protein-design
#2
Ilan Samish
Computational protein design (CPD), a yet evolving field, includes computer-aided engineering for partial or full de novo designs of proteins of interest. Designs are defined by a requested structure, function, or working environment. This chapter describes the birth and maturation of the field by presenting 101 CPD examples in a chronological order emphasizing achievements and pending challenges. Integrating these aspects presents the plethora of CPD approaches with the hope of providing a "CPD 101". These reflect on the broader structural bioinformatics and computational biophysics field and include: (1) integration of knowledge-based and energy-based methods, (2) hierarchical designated approach towards local, regional, and global motifs and the integration of high- and low-resolution design schemes that fit each such region, (3) systematic differential approaches towards different protein regions, (4) identification of key hot-spot residues and the relative effect of remote regions, (5) assessment of shape-complementarity, electrostatics and solvation effects, (6) integration of thermal plasticity and functional dynamics, (7) negative design, (8) systematic integration of experimental approaches, (9) objective cross-assessment of methods, and (10) successful ranking of potential designs...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27913017/marine-anthropogenic-litter-on-british-beaches-a-10-year-nationwide-assessment-using-citizen-science-data
#3
S E Nelms, C Coombes, L C Foster, T S Galloway, B J Godley, P K Lindeque, M J Witt
Growing evidence suggests that anthropogenic litter, particularly plastic, represents a highly pervasive and persistent threat to global marine ecosystems. Multinational research is progressing to characterise its sources, distribution and abundance so that interventions aimed at reducing future inputs and clearing extant litter can be developed. Citizen science projects, whereby members of the public gather information, offer a low-cost method of collecting large volumes of data with considerable temporal and spatial coverage...
November 29, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27910578/liquid-phase-catalytic-reactor-combined-with-measurement-of-hot-electron-flux-and-chemiluminescence
#4
Ievgen I Nedrygailov, Changhwan Lee, Song Yi Moon, Hyosun Lee, Jeong Young Park
Understanding the role of electronically nonadiabatic interactions during chemical reactions on metal surfaces in liquid media is of great importance for a variety of applications including catalysis, electrochemistry, and environmental science. Here, we report the design of an experimental apparatus for detection of the highly excited (hot) electrons created as a result of nonadiabatic energy transfer during the catalytic decomposition of hydrogen peroxide on thin-film metal-semiconductor nanodiodes. The apparatus enables the measurement of hot electron flows and related phenomena (e...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27909415/imagining-change-an-integrative-approach-toward-explaining-the-motivational-role-of-mental-imagery-in-pro-environmental-behavior
#5
Christine Boomsma, Sabine Pahl, Jackie Andrade
Climate change and other long-term environmental issues are often perceived as abstract and difficult to imagine. The images a person associates with environmental change, i.e., a person's environmental mental images, can be influenced by the visual information they come across in the public domain. This paper reviews the literature on this topic across social, environmental, and cognitive psychology, and the wider social sciences; thereby responding to a call for more critical investigations into people's responses to visual information...
2016: Frontiers in Psychology
https://www.readbyqxmd.com/read/27906509/principles-of-brain-development
#6
REVIEW
Joan Stiles
Throughout much of the 20th century, the major models of brain development were strongly deterministic. It was thought that brain development proceeds via a prescribed blueprint that is somehow innately specified in the organism. Contemporary models present a distinctly different view of both inheritance and brain development. First, we do not inherit blueprints or plans, we inherit genes and the cellular machinery for expressing them. Genes carry essential information for creating proteins, but do not determine biological processes or developmental outcomes; the first cells contain the elements necessary for creating proteins based on the information coded in the nucleotide sequences of genes...
December 1, 2016: Wiley Interdisciplinary Reviews. Cognitive Science
https://www.readbyqxmd.com/read/27903053/a-comparison-of-temporal-and-location-based-sampling-strategies-for-global-positioning-system-triggered-electronic-diaries
#7
Tobias Törnros, Helen Dorn, Markus Reichert, Ulrich Ebner-Priemer, Hans-Joachim Salize, Heike Tost, Andreas Meyer-Lindenberg, Alexander Zipf
Self-reporting is a well-established approach within the medical and psychological sciences. In order to avoid recall bias, i.e. past events being remembered inaccurately, the reports can be filled out on a smartphone in real-time and in the natural environment. This is often referred to as ambulatory assessment and the reports are usually triggered at regular time intervals. With this sampling scheme, however, rare events (e.g. a visit to a park or recreation area) are likely to be missed. When addressing the correlation between mood and the environment, it may therefore be beneficial to include participant locations within the ambulatory assessment sampling scheme...
November 21, 2016: Geospatial Health
https://www.readbyqxmd.com/read/27902453/environmental-and-occupational-exposure-to-cadmium-in-iran-a-systematic-review
#8
Leila Tavakkoli, Narges Khanjani
INTRODUCTION: Cadmium (Cd) is one of the heavy metals that have negative effects on human health. In Iran, there is no policy to regularly measure Cd in the general population. However, some studies have been performed on special groups and in different cities of Iran. This paper has reviewed these studies and human contamination levels in Iran. METHOD AND MATERIALS: All accessible electronic information resources such as Scientific Information Database (SID), Magiran, IranMedex, PubMed, Web of Knowledge, Google Scholar and Science Direct were searched, with relevant phrases and their Persian translations...
December 1, 2016: Reviews on Environmental Health
https://www.readbyqxmd.com/read/27901017/daily-intake-and-hazard-index-of-parabens-based-upon-24%C3%A2-h-urine-samples-of-the-german-environmental-specimen-bank-from-1995-to-2012
#9
Rebecca K Moos, Petra Apel, Christa Schröter-Kermani, Marike Kolossa-Gehring, Thomas Brüning, Holger M Koch
In recent years, exposure to parabens has become more of a concern because of evidence of ubiquitous exposure in the general population, combined with evidence of their potency as endocrine disruptors. New human metabolism data from oral exposure experiments enable us to back calculate daily paraben intakes from urinary paraben levels. We report daily intakes (DIs) for six parabens based on 660 24 h urine samples from the German Environmental Specimen Bank collected between 1995 and 2012. Median DI values ranged between 1...
November 30, 2016: Journal of Exposure Science & Environmental Epidemiology
https://www.readbyqxmd.com/read/27901016/relationships-between-arsenic-concentrations-in-drinking-water-and-lung-and-bladder-cancer-incidence-in-u-s-counties
#10
William M Mendez, Sorina Eftim, Jonathan Cohen, Isaac Warren, John Cowden, Janice S Lee, Reeder Sams
Increased risks of lung and bladder cancer have been observed in populations exposed to high levels of inorganic arsenic. However, studies at lower exposures (i.e., less than 100 μg/l in water) have shown inconsistent results. We therefore conducted an ecological analysis of the association between historical drinking water arsenic concentrations and lung and bladder cancer incidence in U.S. counties. We used drinking water arsenic concentrations measured by the U.S. Geological Survey and state agencies in the 1980s and 1990s as proxies for historical exposures in counties where public groundwater systems and private wells are important sources of drinking water...
November 30, 2016: Journal of Exposure Science & Environmental Epidemiology
https://www.readbyqxmd.com/read/27899636/the-monarch-initiative-an-integrative-data-and-analytic-platform-connecting-phenotypes-to-genotypes-across-species
#11
Christopher J Mungall, Julie A McMurry, Sebastian Köhler, James P Balhoff, Charles Borromeo, Matthew Brush, Seth Carbon, Tom Conlin, Nathan Dunn, Mark Engelstad, Erin Foster, J P Gourdine, Julius O B Jacobsen, Dan Keith, Bryan Laraway, Suzanna E Lewis, Jeremy NguyenXuan, Kent Shefchek, Nicole Vasilevsky, Zhou Yuan, Nicole Washington, Harry Hochheiser, Tudor Groza, Damian Smedley, Peter N Robinson, Melissa A Haendel
The correlation of phenotypic outcomes with genetic variation and environmental factors is a core pursuit in biology and biomedicine. Numerous challenges impede our progress: patient phenotypes may not match known diseases, candidate variants may be in genes that have not been characterized, model organisms may not recapitulate human or veterinary diseases, filling evolutionary gaps is difficult, and many resources must be queried to find potentially significant genotype-phenotype associations. Non-human organisms have proven instrumental in revealing biological mechanisms...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27894680/frequency-of-micronuclei-and-other-biomarkers-of-dna-damage-in-populations-exposed-to-dusts-asbestos-and-other-fibers-a-systematic-review
#12
REVIEW
Stefano Bonassi, Mirta Milić, Monica Neri
Airborne particles are small, solid particles projected into the air either by natural forces, or by mechanical or man-made processes, and include fibers and dusts. Their toxicity is usually subsequent to inhalation and can lead to pulmonary dysfunctions and diseases, including cancer. Cytochalasin B blocked micronucleus assay in lymphocytes (L-CBMN) has been shown as a sensitive and reliable technique in assessing genotoxic exposure, An extensive search of the PubMed and Web of Science databases allowed retrieval of 18 articles on occupational or environmental exposure evaluating L-CBMN in subjects exposed to fibers or dusts (asbestos, silica, rockwool, beryllium, tobacco, and wood)...
October 2016: Mutation Research
https://www.readbyqxmd.com/read/27891647/a-roadmap-for-improving-the-representation-of-photosynthesis-in-earth-system-models
#13
Alistair Rogers, Belinda E Medlyn, Jeffrey S Dukes, Gordon Bonan, Susanne von Caemmerer, Michael C Dietze, Jens Kattge, Andrew D B Leakey, Lina M Mercado, Ülo Niinemets, I Colin Prentice, Shawn P Serbin, Stephen Sitch, Danielle A Way, Sönke Zaehle
Accurate representation of photosynthesis in terrestrial biosphere models (TBMs) is essential for robust projections of global change. However, current representations vary markedly between TBMs, contributing uncertainty to projections of global carbon fluxes. Here we compared the representation of photosynthesis in seven TBMs by examining leaf and canopy level responses of photosynthetic CO2 assimilation (A) to key environmental variables: light, temperature, CO2 concentration, vapor pressure deficit and soil water content...
January 2017: New Phytologist
https://www.readbyqxmd.com/read/27890403/isoelectric-points-and-points-of-zero-charge-of-metal-hydr-oxides-50years-after-parks-review
#14
REVIEW
Marek Kosmulski
The pH-dependent surface charging of metal (hydr)oxides is reviewed on the occasion of the 50th anniversary of the publication by G.A. Parks: "Isoelectric points of solid oxides, solid hydroxides, and aqueous hydroxo complex systems" in Chemical Reviews. The point of zero charge (PZC) and isoelectric point (IEP) became standard parameters to characterize metal oxides in aqueous dispersions, and they define adsorption (surface excess) of ions, stability against coagulation, rheological properties of dispersions, etc...
November 5, 2016: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/27889875/development-of-an-educational-program-integrating-concepts-of-genetic-risk-and-preventive-strategies-for-children-with-a-family-history-of-melanoma
#15
Yelena P Wu, Lisa G Aspinwall, Elizabeth Nagelhout, Wendy Kohlmann, Kimberly A Kaphingst, Sheila Homburger, Ryan D Perkins, Douglas Grossman, Garrett Harding, Pamela Cassidy, Sancy A Leachman
Efforts to prevent melanoma, especially for those at elevated risk for the disease, should ideally begin during childhood. However, there are few preventive interventions targeting children who are at higher risk for melanoma due to a family history of the disease. Further, there are no educational interventions that aim to help these at-risk children understand their risk for melanoma and the ways in which preventive behaviors, such as sun protection, can mitigate their risk. The current paper describes a multidisciplinary team's process for creating a developmentally appropriate educational intervention about melanoma risk and prevention for children ages 8-17 years who have a family history of melanoma...
November 26, 2016: Journal of Cancer Education: the Official Journal of the American Association for Cancer Education
https://www.readbyqxmd.com/read/27888927/thermal-behavior-of-human-eye-in-relation-with-change-in-blood-perfusion-porosity-evaporation-and-ambient-temperature
#16
REVIEW
Aasma Rafiq, M A Khanday
Extreme environmental and physiological conditions present challenges for thermal processes in body tissues including multi-layered human eye. A mathematical model has been formulated in this direction to study the thermal behavior of the human eye in relation with the change in blood perfusion, porosity, evaporation and environmental temperatures. In this study, a comprehensive thermal analysis has been performed on the multi-layered eye using Pennes' bio-heat equation with appropriate boundary and interface conditions...
December 2016: Journal of Thermal Biology
https://www.readbyqxmd.com/read/27888923/determination-of-time-of-death-in-forensic-science-via-a-3-d-whole-body-heat-transfer-model
#17
Catherine Bartgis, Alexander M LeBrun, Ronghui Ma, Liang Zhu
This study is focused on developing a whole body heat transfer model to accurately simulate temperature decay in a body postmortem. The initial steady state temperature field is simulated first and the calculated weighted average body temperature is used to determine the overall heat transfer coefficient at the skin surface, based on thermal equilibrium before death. The transient temperature field postmortem is then simulated using the same boundary condition and the temperature decay curves at several body locations are generated for a time frame of 24h...
December 2016: Journal of Thermal Biology
https://www.readbyqxmd.com/read/27888425/an-overview-on-common-aspects-influencing-the-dissipation-pattern-of-pesticides-a-review
#18
Waziha Farha, A M Abd El-Aty, Md Musfiqur Rahman, Ho-Chul Shin, Jae-Han Shim
The common aspects and processes influencing dissipation kinetics of pesticides are determinants of their fate in the environment. Nowadays, with increasing population, the demand for food and fodder crops has also increased. With the development in science and technology, the methods of controlling pests may improve, but the major role played by the environment cannot be altered, i.e. the environmental factors, climatic conditions, and geology of areas under cultivation. Plants play a crucial role in the dissipation kinetics, as they may vary in species and characteristics...
December 0: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27882306/personalized-proteomics-the-future-of-precision-medicine
#19
Trevor T Duarte, Charles T Spencer
Medical diagnostics and treatment has advanced from a one size fits all science to treatment of the patient as a unique individual. Currently, this is limited solely to genetic analysis. However, epigenetic, transcriptional, proteomic, posttranslational modifications, metabolic, and environmental factors influence a patient's response to disease and treatment. As more analytical and diagnostic techniques are incorporated into medical practice, the personalized medicine initiative transitions to precision medicine giving a holistic view of the patient's condition...
2016: Proteomes
https://www.readbyqxmd.com/read/27881558/the-american-heart-association-s-new-institute-for%C3%A2-precision-cardiovascular-medicine
#20
Steven R Houser
"One size does not fit all." These 6 simple words represent the fundamental concept driving an unprecedented initiative of the American Heart Association, the Institute for Precision Cardiovascular Medicine. In the field of heart disease and stroke research, we have seen enormous knowledge advances in recent decades, all of which have led to breakthroughs in our ability to treat and prevent these illnesses. We celebrate these advances because they are allowing us to save and improve more lives than ever before...
November 22, 2016: Circulation
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