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Urine microscopy

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https://www.readbyqxmd.com/read/29692316/extended-screening-for-infectious-diseases-among-newly-arrived-asylum-seekers-from-africa-and-asia-verona-province-italy-april-2014-to-june-2015
#1
Dora Buonfrate, Federico Gobbi, Valentina Marchese, Chiara Postiglione, Geraldo Badona Monteiro, Giovanni Giorli, Giuseppina Napoletano, Zeno Bisoffi
Background and aimManagement of health issues presented by newly-arrived migrants is often limited to communicable diseases even though other health issues may be more prevalent. We report the results of infectious disease screening proposed to 462 recently-arrived asylum seekers over 14 years of age in Verona province between April 2014 and June 2015. Methods: Screening for latent tuberculosis (TB) was performed via tuberculin skin test (TST) and/or QuantiFERON-TB Gold in-tube assay and/or chest X-ray. An ELISA was used to screen for syphilis...
April 2018: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/29689371/large-supramolecular-structures-of-33-mer-gliadin-peptide-activate-toll-like-receptors-in-macrophages
#2
M G Herrera, M Pizzuto, C Lonez, K Rott, A Hütten, N Sewald, J-M Ruysschaert, V I Dodero
Gliadin, an immunogenic protein present in wheat, is not fully degraded by humans and after the normal gastric and pancreatic digestion, the immunodominant 33-mer gliadin peptide remains unprocessed. The 33-mer gliadin peptide is found in human faeces and urine, proving not only its proteolytic resistance in vivo but more importantly its transport through the entire human body. Here, we demonstrate that 33-mer supramolecular structures larger than 220nm induce the overexpression of nuclear factor kappa B (NF-κB) via a specific Toll-like Receptor (TLR) 2 and (TLR) 4 dependent pathway and the secretion of pro-inflammatory cytokines such as IP-10/CXCL10 and TNF-α...
April 21, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29677440/phenotypic-antimicrobial-susceptibility-testing-with-deep-learning-video-microscopy
#3
Hui Yu, Wenwen Jing, Rafael Iriya, Yunze Yang, Karan Syal, Manni Mo, Thomas E Grys, Shelley E Haydel, Shaopeng Wang, Nongjian Tao
Timely determination of antimicrobial susceptibility for a bacterial infection enables precision prescription, shortens treatment time and helps minimize the spread of antibiotic resistant infections. Current antimicrobial susceptibility testing (AST) methods often take several days and thus impede these clinical and health benefits. Here, we present an AST method by imaging freely moving bacterial cells in urine in real time and analyzing the videos with a deep learning algorithm. The deep learning algorithm determines if an antibiotic inhibits a bacterial cell by learning multiple phenotypic features of the cell without the need for defining and quantifying each feature...
April 20, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29674039/reduced-graphene-oxide-supported-gold-dendrite-for-electrochemical-sensing-of-acetaminophen
#4
Shangmin Yu, Haifeng Li, Gengen Li, Liting Niu, Weilu Liu, Xin Di
A new nanocomposite was developed based on reduced graphene oxide (RGO) supported gold dendrite and applied for amperometric detection of acetaminophen. The RGO-gold dendrite composite was prepared by self-assembly of poly (diallyldimethylammonium chloride) (PDDA) functionalized gold dendrite and poly (sodium 4-styrenesulfonate) (PSS) functionalized RGO. The composite electrode material was characterized by Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), Ultraviolet-vis spectroscopy (UV), X-ray diffraction (XRD), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS)...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29667286/molecular-vise-approach-to-create-metal-binding-sites-in-mofs-and-detection-of-biomarker
#5
Yang Wang, Qi Liu, Qin Zhang, Bosi Peng, Hexiang Deng
We report a new approach to create metal-binding site in a series of metal-organic frameworks (MOFs), where tetra-topic carboxylate linker, 4',4'',4''',4''''-methanetetrayltetrabiphenyl-4-carboxylic acid, is partially replaced by a tri-topic carbox-ylate linker, tris(4-carboxybiphenyl)amine, in combination with mono-topic linkers, formic acid, trifluoroacetic acid, ben-zoic acid, isonicotinic acid, 4-chlorobenzoic acid, and 4-nitrobenzoic acid, respectively. The distance between these paired-up linkers can be precisely controlled, ranging from 5...
April 18, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29653387/bovine-uterus-derived-exosomes-improve-developmental-competence-of-somatic-cell-nuclear-transfer-embryos
#6
Fang Qiao, Hui Ge, Xiaonan Ma, Ying Zhang, Zhenzi Zuo, Mengyun Wang, Yong Zhang, Yongsheng Wang
Exosomes widely exist in various tissues and body fluids, including blood, tissue fluid, and urine. In the present study, exosomes were first isolated from the early luteal phase uterus and confirmed through morphological examination, immunofluorescence (IF) staining of special membrane antigen, and Western blot. The effects of exosomes on the developmental competence of somatic cell nuclear transfer (SCNT) embryos were investigated. Transmission electron microscopy results showed that the isolated exsomes were spherical particles with a 50 nm-150 nm diameter...
March 22, 2018: Theriogenology
https://www.readbyqxmd.com/read/29649957/tissue-engineered-neo-urinary-conduit-from-decellularized-trachea
#7
Anirudha Anirudhasingh Gmail Com Singh, David Lee, Harrison Jeong, Christine Yu, Jiuru Li, ChenHao Fang, Praveena Sabnekar, Xiaopu Liu, Takahiro Yoshida, Nikolai Sopko, Trinity Bivalacqua
Decellularized tissues have been increasingly popular for constructing scaffolds for tissue engineering applications due to their beneficial biological compositions and mechanical properties. It is therefore natural to consider decellularized trachea for construction of tissue-engineered trachea as well as other tubular organs. A neo-urinary conduit (NUC) is such a tubular organ that works as a passage for urine removal in bladder cancer patients who need a urinary diversion after their diseased bladder is removed...
April 13, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29643200/the-use-of-automated-urine-microscopy-analysis-in-the-clinical-diagnosis-of-urinary-tract-infection-defining-an-optimal-diagnostic-score-in-an-academic-medical-center-population
#8
Dimard E Foudraine, Martijn P Bauer, Anne Russcher, Elske Kusters, Christa M Cobbaert, Martha T van der Beek, Janneke E Stalenhoef
Background: Automated standardized quantitative urine analysis such as iQ200 analysis is on the rise because of its high accuracy and efficiency compared to traditional urine analysis. Previous research on automated urinalysis focused mainly on predicting culture results but not on the clinical diagnosis of urinary tract infection (UTI). Methods: A retrospective analysis was conducted of consecutive urine samples sent in for culture because of suspected UTI. UTI was defined by expert opinion based on reported symptoms, conventional urine sediment analysis and urine cultures...
April 11, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29629958/guanylyl-cyclase-a-in-both-renal-proximal-tubular-and-vascular-endothelial-cells-protects-the-kidney-against-acute-injury-in-rodent-experimental-endotoxemia-models
#9
Hiroaki Kitamura, Daisuke Nakano, Yoshiharu Sawanobori, Takehiko Asaga, Hideki Yokoi, Motoko Yanagita, Masashi Mukoyama, Takeshi Tokudome, Kenji Kangawa, Gotaro Shirakami, Akira Nishiyama
BACKGROUND: Natriuretic peptides are used, based on empirical observations, in intensive care units as antioliguric treatments. We hypothesized that natriuretic peptides prevent lipopolysaccharide-induced oliguria by activating guanylyl cyclase A, a receptor for natriuretic peptides, in proximal tubules and endothelial cells. METHODS: Normal Sprague-Dawley rats and mice lacking guanylyl cyclase A in either endothelial cells or proximal tubular cells were challenged with lipopolysaccharide and assessed for oliguria and intratubular flow rate by intravital imaging with multiphoton microscopy...
April 9, 2018: Anesthesiology
https://www.readbyqxmd.com/read/29627526/role-of-akr1c3-in-renal-injury-and-glibenclamide-is-anti-inflammatory-in-preeclamptic-rats
#10
Cheng-Juan Sun, Ya Jin, Wei-Yuan Zhang, Lin Li, Xiao-Wei Liu
Proteinuria is a common adverse effect of renal injury in preeclampsia. To explore the effects of AKR1C3 in renal injury due to preeclampsia and to determine an optimal method to prevent proteinuria, glibenclamide treatment was used in unrestrained Wistar rats exposed to N-nitro-L-arginine methyl ester hydrochloride (L-NAME). Successful rat models for preeclampsia were confirmed based on mean arterial pressure, a 24-h protein urine test, and by observing the structure of the kidney by transmission electron microscopy (TEM)...
April 5, 2018: Gene
https://www.readbyqxmd.com/read/29624903/lupus-nephritis-low-urinary-dnase-i-levels-reflect-loss-of-renal-dnase-i-and-may-be-utilized-as-a-biomarker-of-disease-progression
#11
Hege L Pedersen, Kjersti D Horvei, Dhivya Thiyagarajan, Gudrun E Norby, Natalya Seredkina, Gabriella Moroni, Gro Ø Eilertsen, Hallvard Holdaas, Erik H Strøm, Gunnstein Bakland, Pier-Luigi Meroni, Ole P Rekvig
Renal DNase I is lost in advanced stages of lupus nephritis. Here, we determined if loss of renal DNase I reflects a concurrent loss of urinary DNase I, and whether absence of urinary DNase I predicts disease progression. Mouse and human DNase I protein and DNase I endonuclease activity levels were determined by western blot, gel, and radial activity assays at different stages of the murine and human forms of the disease. Cellular localization of DNase I was analyzed by immunohistochemistry, immunofluorescence, confocal microscopy, and immunoelectron microscopy...
February 21, 2018: Journal of Pathology. Clinical Research
https://www.readbyqxmd.com/read/29619876/inhibitory-effects-of-taraxasterol-and-aqueous-extract-of-taraxacum-officinale-on-calcium-oxalate-crystallization-in-vitro-study
#12
Mahboubeh Yousefi Ghale-Salimi, Maryam Eidi, Nasser Ghaemi, Ramezan Ali Khavari-Nejad
OBJECTIVE: We investigated and compared the effects of taraxasterol, aqueous extract of T. officinale (AET) aerial part, and potassium citrate (PC) on calcium oxalate (CaOx) crystallization in vitro. MATERIALS AND METHODS: CaOx crystallization was induced by adding sodium oxalate to synthetic urine. Taraxasterol (2.5, 5, 7.5 and 12.5 μg/mL), extract (1, 2, 4 and 8 mg/mL), and PC (100, 150, 200 and 350 mg/mL) were subjected to anti-crystallization activities...
November 2018: Renal Failure
https://www.readbyqxmd.com/read/29594841/a-two-wavelength-fluorescence-recovery-method-for-the-simultaneous-determination-of-aureomycin-and-oxytetracycline-by-using-gold-nanocrystals-modified-with-serine-and-11-mercaptoundecanoic-acid
#13
Yaning Mao, Yufeng Wu, Yongyin Nie, Jun Wang, Yuanyuan Liu, Shenghao Xu, Xiliang Luo
A method is described for rapid (1 h) synthesis of gold nanoclusters (AuNCs) co-functionalized with serine and 11-mercaptoundecanoic acid. The co-functionalized AuNCs exhibit good stability towards temperature, pH values, and over time. They were characterized by atomic force microscopy, high-resolution transmission electron microscopy, dynamic light scattering, and by fluorescence, IR and X-ray photoelectron spectroscopies. The fluorescence of the AuNCs is quenched by Hg(II) and restored on subsequent addition of aureomycin (CTC) or oxytetracycline (OTC)...
March 14, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29577510/successful-treatment-with-steroid-and-cyclosporine-a-in-a-patient-with-immunoglobulin-a-proliferative-glomerulonephritis-with-monoclonal-immunoglobulin-deposits
#14
Keisuke Sato, Shiho Makabe, Yuko Iwabuchi, Kaori Kojima, Masayo Sato, Hiroshi Kataoka, Takahito Moriyama, Sekiko Taneda, Ken Tsuchiya, Kosaku Nitta, Toshio Mochizuki
We report a case of glomerulonephritis with monoclonal immunoglobulin (Ig) A deposits as a form of monoclonal gammopathy of renal significance (MGRS) caused by monoclonal immunoglobulins without blood disorders in a 41-year-old woman. She developed lower leg oedema and was hospitalized because of nephrotic syndrome. Serum and urine were negative for M protein, and the free light chain κ/λ ratio was within the normal range. Renal histopathological findings included mesangial proliferation, endocapillary cell proliferation, and a double-contour appearance of the capillary walls...
March 25, 2018: Nephrology
https://www.readbyqxmd.com/read/29567183/simultaneous-determination-of-isoproterenol-acetaminophen-folic-acid-propranolol-and-caffeine-using-a-sensor-platform-based-on-carbon-black-graphene-oxide-copper-nanoparticles-and-pedot-pss
#15
Ademar Wong, Anderson Martin Santos, Tiago Almeida Silva, Orlando Fatibello-Filho
We explored the use of carbon black (CB), graphene oxide (GO), copper nanoparticles (CuNPs) and poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) as electrode materials for the simultaneous determination of isoproterenol, acetaminophen, folic acid, propranolol and caffeine. The designed nanostructured surface was widely characterized by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), contact angle measurements and electrochemistry. From electrochemical characterization assays carried out towards the potassium ferricyanide redox probe, fast electron transfer kinetics and a considerably higher electroactive surface area were observed for the modified electrodic surface based on CB, GO, CuNPs and PEDOT:PSS film...
June 1, 2018: Talanta
https://www.readbyqxmd.com/read/29567154/facile-preparation-and-characterization-of-new-green-emitting-carbon-dots-for-sensitive-and-selective-off-on-detection-of-fe-3-ion-and-ascorbic-acid-in-water-and-urine-samples-and-intracellular-imaging-in-living-cells
#16
Mojtaba Shamsipur, Karam Molaei, Fatemeh Molaabasi, Mohsen Alipour, Naader Alizadeh, Saman Hosseinkhani, Morteza Hosseini
Carbon dots (CDs) have gained great attention as multifunctional materials because of their interesting properties and general applicability. However, there are some reports for the preparation of highly luminescent green-emitting CDs (G-CDs), although these reports seem not to be extensible. Herein, new G-CDs (quantum yield: 27.2%) were synthesized from a facile hydrothermal treatment of p-aminosalicylic acid and ethylene glycol dimethacrylate as both carbon and nitrogen source and cross-linking agent, respectively...
June 1, 2018: Talanta
https://www.readbyqxmd.com/read/29565789/improved-contrast-microscopy-modification-of-bright-field-for-urine-sediment-visualisation
#17
Vani Chandrashekar
BACKGROUND: Phase contrast microscopy is the recommended technique for urine sediment examination. Bright field microscopy does not differentiate objects with slight changes in the refractive index and hence phase contrast is a superior alternative. METHODS: In this article, we describe a novel method to improve contrast in bright field microscopy. A strategically placed disc of specific dimensions enhances the diffraction of rays by Fresnel principle causing a shift in wavelength in the rays which are perceived as differences in contrast by the eye due to constructive and destructive interference...
March 28, 2018: Diagnosis
https://www.readbyqxmd.com/read/29560841/surveillance-of-intestinal-schistosomiasis-during-control-a-comparison-of-four-diagnostic-tests-across-five-ugandan-primary-schools-in-the-lake-albert-region
#18
Hajri Al-Shehri, Artemis Koukounari, Michelle C Stanton, Moses Adriko, Moses Arinaitwe, Aaron Atuhaire, Narcis B Kabatereine, J Russell Stothard
Programmatic surveillance of intestinal schistosomiasis during control can typically use four diagnostic tests, either singularly or in combination, but these have yet to be cross-compared directly. Our study assembled a complete diagnostic dataset, inclusive of infection intensities, from 258 children from five Ugandan primary schools. The schools were purposely selected as typical of the endemic landscape near Lake Albert and reflective of high- and low-transmission settings. Overall prevalence was: 44.1% (95% CI 38...
March 21, 2018: Parasitology
https://www.readbyqxmd.com/read/29560055/high-prevalence-of-sterile-pyuria-in-the-setting-of-sexually-transmitted-infection-in-women-presenting-to-an-emergency-department
#19
Stacia B Shipman, Chelsea R Risinger, Crystalle M Evans, Chelsey D Gilbertson, David E Hogan
Introduction: The clinical presentations for sexually transmitted infections (STI) and urinary tract infections (UTI) often overlap, and symptoms of dysuria and urinary frequency/urgency occur with both STIs and UTIs. Abnormal urinalysis (UA) findings and pyuria are common in both UTIs and STIs, and confirmatory urine cultures are not available to emergency clinicians to aid in decision-making regarding prescribing antibiotics for UTIs. The objective of this study was to determine the frequency of sterile pyuria in women with confirmed STIs, as well as whether the absolute number of leukocytes on microscopy or nitrite on urine dipstick correlated with positive urine cultures in patients with confirmed STIs...
March 2018: Western Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29556674/recalcitrant-chronic-bladder-pain-and-recurrent-cystitis-but-negative-urinalysis-what-should-we-do
#20
Sheela Swamy, William Barcella, Maria De Iorio, Kiren Gill, Rajvinder Khasriya, Anthony S Kupelian, Jennifer L Rohn, James Malone-Lee
PURPOSE: Lower urinary tract symptoms (LUTS) may be associated with chronic urinary tract infection (UTI) undetected by routine diagnostic tests. Antimicrobial therapy might confer benefit for these patients. MATERIALS AND METHODS: Over 10 years, we treated patients with chronic LUTS. Pyuria was adopted as the principal biomarker of infection. Urinary leucocyte counts were recorded from microscopy of fresh midstream urine (MSU) samples. Antibiotics were prescribed and the prescription adjusted to achieve a measurable clinical response and a reduction in pyuria...
March 20, 2018: International Urogynecology Journal
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