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Urine microscopy

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https://www.readbyqxmd.com/read/29043360/synthesis-photophysical-and-biological-properties-of-a-new-oxazolone-fluorescent-probe-for-bioimaging-an-experimental-and-theoretical-study
#1
Przemysław Krawczyk, Beata Jędrzejewska, Piotr Cysewski, Tomasz Janek
In this study, a new oxazolone derivative 4-{N,N-bis[2-phenyl-4-benzylidene-1,3-oxazol-5(4H)-one]amino}benzaldehyde (PB3) was synthesized and investigated as a fluorescent dye. The spectroscopic properties in different solvents were thoroughly studied. The experimental data were supported by quantum-chemical calculations using density functional theory. Measurements and theoretical calculations showed that the PB3 dye is characterized by non-monotonic solvatochromism, a strongly polar charge transfer excited state, a large Stokes' shift, a high fluorescence quantum yield and a high fluorescence lifetime...
October 18, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29035449/performance-verification-of-the-iris-iq200-sprint-automated-urine-microscopy-analyzer-in-a-hospital-routine-laboratory
#2
Ming Cui, Shaoqing Ju, Yingjuan Shi, Rongrong Jing
BACKGROUND: Systematic performance verification is required before a laboratory can introduce a new measurement procedure for reporting results of patient testing. The aim of this study was to determine whether a new Iris iQ200 Sprint automated urine microscopy analyzer (iQ200 Sprint) could be incorporated into our routine laboratory. METHODS: A total of 421 fresh urine samples were selected from the Affiliated Hospital of Nantong University, including those from healthy individuals and those with a variety of abnormalities to ensure a wide range of results...
October 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29035286/urocytological-evaluation-of-pemphigus-patients-on-long-term-cyclophosphamide-therapy-a-cross-sectional-study
#3
Sujay Khandpur, Saurabh Singh, Saumyaranjan Mallick, Vinod Kumar Sharma, Venkat Iyer, Amlesh Seth, Mahesh Kumawat
BACKGROUND: Cyclophosphamide therapy is associated with several urological complications including urinary bladder malignancy. Data on urologic complications of chronic cyclophosphamide therapy for dermatologic conditions is not available. OBJECTIVES: To study the urocytological profile of pemphigus patients on long-term cyclophosphamide therapy. MATERIALS AND METHODS: In a cross-sectional study, consecutive patients who had received cyclophosphamide therapy for pemphigus for more than 12 months were included...
November 2017: Indian Journal of Dermatology, Venereology and Leprology
https://www.readbyqxmd.com/read/29035239/diagnosis-of-trichomoniasis-in-male-patients-on-performing-nested-polymerase-chain-reaction
#4
T Mutlu Yar, Mehmet Karakuş, Seray Töz, Aysun Bay Karabulut, Yusuf Özbel, Metin Atambay
OBJECTIVE: Trichomoniasis is a parasitic infection that occurs with the settlement of Trichomonas vaginalis in female and male urinary and reproductive tracts. This infection is generally asymptomatic in males, and males are thought to be a carrier for the transmission of infection. In this study, our aim was to detect trichomoniasis using nested polymerase chain reaction among males who were referred to a hospital with suspected urinary tract infection. METHODS: Urine samples were collected from 138 male patients between 18 and 50 years of age who were referred with suspected urinary system infection to the Urology Outpatient Clinic at Malatya University Medical Center Malatya between December 2013 and May 2014...
September 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29029307/droplet-digital-pcr-diagnosis-of-human-schistosomiasis-parasite-cell-free-dna-detection-in-diverse-clinical-samples
#5
Kosala G Weerakoon, Catherine A Gordon, Gail M Williams, Pengfei Cai, Geoffrey N Gobert, Remigio M Olveda, Allen G Ross, David U Olveda, Donald P McManus
Background: Schistosomiasis japonica remains a major public health and socio-economic concern in Southeast-Asia. Sensitive and accurate diagnostics can play a pivotal role in achieving disease elimination goals. Methods: We previously reported a novel droplet digital-PCR (ddPCR) assay targeting the mitochondrial gene nad1 to diagnose schistosomiasis japonica. The tool identified both pre-patent and patent infections using Schistosoma japonicum DNA isolated from serum, urine, salivary glands and faeces in a murine model...
September 27, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29017265/antibiotic-overconsumption-in-pregnant-women-with-urinary-tract-symptoms-in-uganda
#6
Musa Sekikubo, Karolina Hedman, Florence Mirembe, Annelie Brauner
Background: Urinary tract infections (UTIs) are one of the most common bacterial infections in women. During pregnancy physiological changes, like frequency, mimic UTI symptoms, and therefore bacteriological cultures are needed to confirm the diagnosis. However, in developing countries antibiotic therapy is commonly initiated without culture confirmation. Methods: We investigated the prevalence of bacteriuria among pregnant women with and without UTI symptoms in Uganda...
August 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28992005/characterization-of-exosomes-from-body-fluids-of-dairy-cows
#7
Y Q Koh, H N Peiris, K Vaswani, S Meier, C R Burke, K A Macdonald, J R Roche, F Almughlliq, B J Arachchige, S Reed, M D Mitchell
Exosomes are a specific subpopulation of extracellular vesicles that are widely released by cells of different origins with divergent functions that make their way into body fluids that can be conveniently sampled. In the current study, we isolated and evaluated exosomes from concurrently collected samples of milk, plasma, saliva, and urine from a group of 6 pregnant Holstein-Friesian dairy cows (aged 7 mo, 174 to 203 d of gestation). The cows had BCS of 3.5 to 5.25 (on a scale of 1 to 10), and the milk production for the season to the time of sampling ranged between 5,118 and 6,959 kg...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28988177/fabry-disease-due-to-d313y-and-novel-gla-mutations
#8
Konstantinos Koulousios, Konstantinos Stylianou, Panagiotis Pateinakis, Maria Zamanakou, Gedeon Loules, Eleni Manou, Parthena Kyriklidou, Christos Katsinas, Alexandra Ouzouni, John Kyriazis, Matthaios Speletas, Anastasios E Germenis
OBJECTIVES: Our aim is to report four novel α-gal A gene (GLA) mutations resulting in Fabry disease (FD) and provide evidence of pathogenicity of the D313Y mutation regarding which contradictory data have been presented in the literature. SETTING AND PARTICIPANTS: Twenty-five family members of nine unrelated patients with definite FD diagnosis, 10 clinically suspected cases and 18 members of their families were included in this polycentric cohort study. PRIMARY AND SECONDARY OUTCOME MEASURES: Genotyping and measurement of lyso-Gb3 was performed in all individuals...
October 6, 2017: BMJ Open
https://www.readbyqxmd.com/read/28985483/pipette-tip-dummy-molecularly-imprinted-solid-phase-extraction-of-bisphenol-a-from-urine-samples-and-analysis-by-gas-chromatography-coupled-to-mass-spectrometry
#9
Tamires Amabile Valim Brigante, Luis Felippe Cabral Miranda, Israel Donizeti de Souza, Vinicius Ricardo Acquaro Junior, Maria Eugênia Costa Queiroz
Molecularly imprinted polymers (MIPs) were synthesized and used as sorbent for Bisphenol A (BPA) pipette tip solid-phase microextraction from urine samples and BPA analysis by gas chromatography coupled to mass spectrometry (GC-MS). The MIPs were synthesized by the sol-gel methodology. Aminopropyltriethoxysilane (APTES) and tetraethyl orthosilicate (TEOS) were used as functional monomer and cross-linking reagent, respectively. BPA and tetrabromobisphenol A (TBBPA) were evaluated as template during MIP synthesis...
September 27, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28984337/primary-splenic-pregnancy-a-rare-but-imperative-cause-of-hemoperitoneum-case-report-and-review-of-literature
#10
Ruchi Rathore, Shilpi Shilpi, Ratna Chopra, Namrata Nargotra
Primary splenic pregnancy is an extremely rare form of extratubal ectopic pregnancy. These cases often cause splenic rupture in very early course of their gestation thereby presenting with hemoperitoneum in emergencies. Owing to the higher risk of exsanguination and death caused by hemoperitoneum, it is essential to diagnose these cases for proper management and better prognosis of the patients. We present the case of a 23-year-old female, gravida 2, para 1, live issue 1 presenting to the emergency outpatient department with acute abdomen and hemoperitoneum...
October 6, 2017: Türk Patoloji Dergisi
https://www.readbyqxmd.com/read/28978534/nmr-spectroscopy-and-electron-microscopy-identification-of-metabolic-and-ultrastructural-changes-to-the-kidney-following-ischemia-reperfusion-injury
#11
Tafadzwa Chihanga, Qing Ma, Jenna D Nicholson, Hannah N Ruby, Richard E Edelmann, Prasad Devarajan, Michael A Kennedy
Cellular, molecular, and ultrastructural nephron changes associated with ischemia reperfusion injury-induced acute kidney injury (IRI-AKI) are not completely understood. Here, a multidisciplinary study was used to identify nephron changes in a mouse model of IRI-AKI. Histological analyses indicated distended Bowman's glomerular spaces and proximal and distal tubules. Increased filtrate volume in nephrons was caused by reduced water reabsorption by severely damaged proximal tubule brush borders and blocked flow of filtrate into collecting tubules by mucoprotein casts in distal tubules...
October 4, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28975104/proliferative-glomerulonephritis-with-monoclonal-igg-deposits-an-unusual-cause-of-de-novo-disease-in-kidney-allograft
#12
Sabiha M Hussain, Kalathil K Sureshkumar
BACKGROUND: Proliferative glomerulonephritis with monoclonal IgG deposits (PGNMID) is a newly described and rare entity that can develop in native and very rarely in transplanted kidneys. We present a patient who developed de novo PGNMID in the kidney allograft along with a review of the literature. CASE PRESENTATION: A 38-year old female with type 1 diabetes who underwent successful simultaneous pancreas-kidney (SPK) transplantation 6 years earlier presented with rising serum creatinine, nephrotic range proteinuria and microhematuria...
July 2017: Journal of Nephropathology
https://www.readbyqxmd.com/read/28971220/lower-urinary-tract-symptoms-that-predict-microscopic-pyuria
#13
Rajvinder Khasriya, William Barcella, Maria De Iorio, Sheela Swamy, Kiren Gill, Anthony Kupelian, James Malone-Lee
INTRODUCTION AND HYPOTHESIS: Urinary dipsticks and culture analyses of a mid-stream urine specimen (MSU) at 10(5) cfu ml(-1) of a known urinary pathogen are considered the gold standard investigations for diagnosing urinary tract infection (UTI). However, the reliability of these tests has been much criticised and they may mislead. It is now widely accepted that pyuria (≥1 WBC μl(-1)) detected by microscopy of a fresh unspun, unstained specimen of urine is the best biological indicator of UTI available...
October 2, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28970097/urinary-schistosomiasis-in-school-aged-children-of-two-rural-endemic-communities-in-edo-state-nigeria
#14
Rukeme M Noriode, Emmanuel T Idowu, Olubunmi A Otubanjo, Margaret A Mafe
BACKGROUND: Urinary schistosomiasis is endemic in many rural communities of Nigeria and school aged children are mostly affected. A cross-sectional study was carried out to assess the prevalence and intensity of urinary schistosomiasis infection among 251 school aged children in two communities of Ovia South West LGA of Edo State, Nigeria, as well as their knowledge on the control/elimination measures. METHODS: Urine samples were collected and examined by microscopy using filtration technique...
September 29, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28962156/pilot-application-of-lipoxin-a4-analog-and-lipoxin-a4-receptor-agonist-in-asthmatic-children-with-acute-episodes
#15
Xia Kong, Sheng-Hua Wu, Li Zhang, Xiao-Qing Chen
Previous studies have demonstrated that lipoxin A4 (LXA4) analogs blocked both airway hyper-responsiveness and pulmonary inflammation in a murine model of asthma. The present pilot study investigated the initial efficacy and safety of inhaled 5(S),6(R)-LXA4 methyl ester and BML-111, a LXA4 agonist, in the treatment of asthmatic children with acute episodes. A total of 50 asthmatic children diagnosed with acute moderate asthma were randomly assigned into groups and subjected to an inhalation challenge with pulmicort (n=10), ventolin (n=10), 5(S),6(R)-LXA4 methyl ester (n=10), BML-111 (n=10) or normal saline as a placebo (n=10)...
September 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28958893/mucoadhesive-maleimide-functionalised-liposomes-for-drug-delivery-to-urinary-bladder
#16
Daulet B Kaldybekov, Prasopchai Tonglairoum, Praneet Opanasopit, Vitaliy V Khutoryanskiy
Intravesical drug administration is used to deliver chemotherapeutic agents via a catheter to treat bladder cancer. The major limitation of this treatment is poor retention of the drug in the bladder due to periodic urine voiding. In this work, maleimide-functionalised PEGylated liposomes (PEG-Mal) were explored as mucoadhesive vehicles for drug delivery to the urinary bladder. The retention of these liposomes on freshly excised porcine bladder mucosa in vitro was compared with conventional liposomes, PEGylated liposomes, two controls (dextran and chitosan), and evaluated through Wash Out50 (WO50) values...
September 27, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28946397/fabrication-of-magnetic-fe3o4-nsio2-msio2-nh2-core-shell-mesoporous-nanocomposite-and-its-application-for-highly-efficient-ultrasound-assisted-dispersive-%C3%A2%C2%B5spe-spectrofluorimetric-detection-of-ofloxacin-in-urine-and-plasma-samples
#17
Roya Mirzajani, Nahid Pourreza, Jafar Burromandpiroze
In this research, a sensitive, simple and rapid ultrasound assisted dispersive micro solid-phase extraction (USAD-µSPE) was developed using a synthesized core-shell magnetic mesoporous nanocomposite (Fe3O4@nSiO2@mSiO2-NH2) as an efficient adsorbent for the preconcentration and spectrofluorometric determination of ofloxacin (OFL) in biological samples. The synthesized adsorbent was characterized using FT-IR spectroscopy, transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), energy dispersive X-ray (EDX) spectroscopy, thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) analysis...
January 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28936266/a-novel-method-for-the-isolation-of-extracellular-vesicles-and-rna-from-urine
#18
Anna Markowska, R Scott Pendergrast, J Stephen Pendergrast, P Shannon Pendergrast
The discovery of urinary extracellular biomarkers has been impeded by the lack of efficient methods for the isolation of extracellular vesicles (EVs: exosomes and microvesicles) and extracellular nucleic acid (RNA and DNA) from urine. Ultracentrifugation (UC), considered the gold standard for vesicle isolation from many biofluids, is efficacious but laborious, and, like most commercially available methods, is unable to isolate enough material from small volumes for protein or RNA-based biomarker discovery. We have developed a novel precipitation method for the isolation of EVs and nucleic acids from urine...
January 2017: J Circ Biomark
https://www.readbyqxmd.com/read/28934365/murine-glomerular-transcriptome-links-endothelial-cell-specific-molecule-1-deficiency-with-susceptibility-to-diabetic-nephropathy
#19
Xiaoyi Zheng, Fariborz Soroush, Jin Long, Evan T Hall, Puneeth K Adishesha, Sanchita Bhattacharya, Mohammad F Kiani, Vivek Bhalla
Diabetic nephropathy (DN) is the leading cause of kidney disease; however, there are no early biomarkers and no cure. Thus, there is a large unmet need to predict which individuals will develop nephropathy and to understand the molecular mechanisms that govern this susceptibility. We compared the glomerular transcriptome from mice with distinct susceptibilities to DN at four weeks after induction of diabetes, but before histologic injury, and identified differential regulation of genes that modulate inflammation...
2017: PloS One
https://www.readbyqxmd.com/read/28932401/practical-approach-to-detection-and-management-of-acute-kidney-injury-in-critically-ill-patient
#20
REVIEW
Vahid Mohsenin
BACKGROUND: Acute kidney injury (AKI) is a common complication in critically ill patients and is associated with high morbidity and mortality. This paper provides a critical review of the etiologies of AKI and a systematic approach toward its diagnosis and management with emphasis on fluid volume assessment and the use of urine biochemical profile and microscopy in identifying the nature and the site of kidney injury. MATERIALS AND METHODS: The search of PubMed and selection of papers had employed observational designs or randomized control trials relevant to AKI...
2017: Journal of Intensive Care
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