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Urine microscopy

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https://www.readbyqxmd.com/read/28821965/increased-endocytosis-of-magnetic-nanoparticles-into-cancerous-urothelial-cells-versus-normal-urothelial-cells
#1
Jasna Lojk, Vladimir Boštjan Bregar, Klemen Strojan, Samo Hudoklin, Peter Veranič, Mojca Pavlin, Mateja Erdani Kreft
The blood-urine barrier is the tightest and most impermeable barrier in the body and as such represents a problem for intravesical drug delivery applications. Differentiation-dependent low endocytotic rate of urothelial cells has already been noted; however, the differences in endocytosis of normal and cancer urothelial cells have not been exploited yet. Here we analysed the endocytosis of rhodamine B isothiocyanate-labelled polyacrylic acid-coated cobalt ferrite nanoparticles (NPs) in biomimetic urothelial in vitro models, i...
August 18, 2017: Histochemistry and Cell Biology
https://www.readbyqxmd.com/read/28814668/injury-induced-actin-cytoskeleton-reorganization-in-podocytes-revealed-by-super-resolution-microscopy
#2
Hani Y Suleiman, Robyn Roth, Sanjay Jain, John E Heuser, Andrey S Shaw, Jeffrey H Miner
The architectural integrity of tissues requires complex interactions, both between cells and between cells and the extracellular matrix. Fundamental to cell and tissue homeostasis are the specific mechanical forces conveyed by the actomyosin cytoskeleton. Here we used super-resolution imaging methods to visualize the actin cytoskeleton in the kidney glomerulus, an organized collection of capillaries that filters the blood to make the primary urine. Our analysis of both mouse and human glomeruli reveals a network of myosin IIA-containing contractile actin cables within podocyte cell bodies and major processes at the outer aspects of the glomerular tuft...
August 17, 2017: JCI Insight
https://www.readbyqxmd.com/read/28814579/hepatosplenic-schistosomiasis-playing-hide-and-seek-with-an-elusive-parasite
#3
Martin Baekby, Henning Glerup, Katrine Stribolt, Britta Tarp
A 27-year-old man of Eritrean origin presented with persistent left-sided abdominal pain. Initial investigation showed signs of liver fibrosis, portal hypertension and splenomegaly. A diagnosis of hepatosplenic schistosomiasis was suspected on grounds of elevated total IgE, grey area antischistosomiasis antibodies and the high endemic status of his native country. However, repeated microscopy of faecal and urine samples, as well as rectal biopsies, failed to demonstrate schistosomal eggs. Finally, the diagnosis of hepatosplenic schistosomiasis was established through demonstration of a Schistosoma mansoni egg in a liver biopsy taken in an attempt to clarify the cause of the above findings...
August 16, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28799315/glucose-6-phosphate-dehydrogenase-deficiency-unveiled-by-diabetic-ketoacidosis-a-dual-dilemma
#4
Pankti Mehta, Vishal Srivastav, Priya Bhate, Vishal Gupta, Milind Y Nadkar
An 18 year old male, known case of Type 1 Diabetes Mellitus was admitted in view of diabetic ketoacidosis. With normalization of blood sugars patient developed gross reddish discoloration of urine. Urine routine microscopy did not reveal RBCs or RBC casts. Peripheral blood smear revealed bite cells, Heinz bodies and spherocytes. Thus a diagnosis of hemolytic anemia with hemoglobinuria was made. Patient's glucose-6-phosphate dehydrogenase (G6PD) levels were below the normal range. G6PD, an enzyme of the HMP shunt, is the most common enzyme defect causing hemolytic anemia...
August 2017: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/28798722/autonomic-neuropathy-and-albuminocytologic-dissociation-in-cerebrospinal-fluid-as-the-presenting-features-of-primary-amyloidosis-a-case-report
#5
Jingjing Li, Yi Li, Hongbing Chen, Shihui Xing, Huiyu Feng, Dawei Liu, Dilong Wang, Jinsheng Zeng, Yuhua Fan
OBJECTIVE: Primary amyloidosis is a disease with a poor prognosis and multi-organ involvement. Here, we report the clinical and pathological features of a patient with primary amyloidosis featuring autonomic neuropathy as the initial symptom and albuminocytologic dissociation in the cerebrospinal fluid (CSF). METHODS: The patient was a 60-year-old Chinese male with numbness, orthostatic hypotension, and gastrointestinal symptoms. For diagnosis, we performed an electromyogram (EMG), lumbar puncture, Bence Jones protein urine test, serum electrophoresis blood test, sural nerve and rectal membrane biopsies, transthyretin (TTR) gene sequencing, and bone marrow puncture...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28793922/comparison-of-novel-and-standard-diagnostic-tools-for-the-detection-of-schistosoma-mekongi-infection-in-lao-people-s-democratic-republic-and-cambodia
#6
Youthanavanh Vonghachack, Somphou Sayasone, Virak Khieu, Robert Bergquist, Govert J van Dam, Pytsje T Hoekstra, Paul L A M Corstjens, Beatrice Nickel, Hanspeter Marti, Jürg Utzinger, Sinuon Muth, Peter Odermatt
BACKGROUND: Given the restricted distribution of Schistosoma mekongi in one province in Lao People's Democratic Republic (Lao PDR) and two provinces in Cambodia, together with progress of the national control programmes aimed at reducing morbidity and infection prevalence, the elimination of schistosomiasis mekongi seems feasible. However, sensitive diagnostic tools will be required to determine whether elimination has been achieved. We compared several standard and novel diagnostic tools in S...
August 10, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28774945/arteriolar-hyalinosis-predicts-increase-in-albuminuria-and-gfr-decline-in-normo-and-microalbuminuric-japanese-patients-with-type-2-diabetes
#7
Tatsumi Moriya, Kazuki Omura, Madoka Matsubara, Yuki Yoshida, Kei Hayama, Motoshi Ouchi
OBJECTIVE: This study investigated the association between renal histology, as assessed by morphometric analysis using light (LM) and electron (EM) microscopy, and changes in urinary albumin excretion (UAE) and glomerular filtration rate (GFR) in Japanese people with type 2 diabetes in the early stages of diabetic nephropathy. RESEARCH DESIGN AND METHODS: We performed percutaneous renal biopsies in 29 patients with type 2 diabetes (22 men, mean ± SD age 49 ± 10 years and GFR 119 ± 27 mL/min/1...
August 3, 2017: Diabetes Care
https://www.readbyqxmd.com/read/28771134/evaluation-of-the-cobas-u-701-microscopy-analyser-compared-with-urine-culture-in-screening-for-urinary-tract-infection
#8
Si Hyun Kim, Sae Am Song, Sang-Hwa Urm, Joong-Ki Kook, Hye Ran Kim, Dongeun Yong, Jun Yong Choi, Jeong Hwan Shin
PURPOSE: A new automated Cobas u 701 microscopy analyser for urine sediment examination was introduced. The aim of this study was to evaluate the analyser in comparison with urine culture in screening for urinary tract infection (UTI). METHODOLOGY: A total of 852 urine specimens submitted for culture were included in this study. Urine sediment examination was performed using the Cobas u 701 microscopy analyser. The results of the bacteria (BAC) and yeast (YEA) analyses were compared with the results from urine culture as a method for UTI screening...
August 4, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28763127/european-veterinary-renal-pathology-service-a-survey-over-a-7-year-period-2008-2015
#9
L Aresu, V Martini, S L Benali, C Brovida, R E Cianciolo, R Dalla Riva, D Trez, J J Van Der Lugt, A Van Dongen, E Zini
BACKGROUND: The European Veterinary Renal Pathology Service (EVRPS) is the first Web-based registry for canine renal biopsy specimens in Europe. HYPOTHESIS/OBJECTIVES: The aim was to verify whether differences exist between the clinical and laboratory presentation of dogs with nephropathy according to renal pathological findings, as defined by light and electron microscopy of renal biopsy specimens submitted to EVRPS. ANIMALS: Renal biopsy specimens of dogs were collected from the archive of the service (n = 254)...
August 1, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28758790/the-accuracy-of-the-sysmex-uf-1000i-in-urine-bacterial-detection-compared-with-the-standard-urine-analysis-and-culture
#10
Patrick Erdman, Brian Anderson, J Christopher Zacko, Kirk Taylor, Keri Donaldson
CONTEXT: - Urinary tract infections are characterized by the presence of microbial pathogens within the urinary tract. They represent one of the most common infections in hospitalized and clinic patients. OBJECTIVES: - To model the parameters of the Sysmex UF-1000i to the gold standard, urine culture, and to compare the detection of dipstick leukocyte esterase and nitrates to urine cultures and UF-1000i results. DESIGN: - Data were compared from urine samples collected in sterile containers for bacterial culture and microscopic analysis...
July 31, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28750602/mechanical-induction-of-bi-directional-orientation-of-primary-porcine-bladder-smooth-muscle-cells-in-tubular-fibrin-poly-vinylidene-fluoride-scaffolds-for-ureteral-and-urethral-repair-using-cyclic-and-focal-balloon-catheter-stimulation
#11
Volker Seifarth, Joachim O Grosse, Matthias Gossmann, Heinz Peter Janke, Patrick Arndt, Sabine Koch, Matthias Epple, Gerhard M Artmann, Aysegül Temiz Artmann
To restore damaged organ function or to investigate organ mechanisms, it is necessary to prepare replicates that follow the biological role model as faithfully as possible. The interdisciplinary field of tissue engineering has great potential in regenerative medicine and might overcome negative side effects in the replacement of damaged organs. In particular, tubular organ structures of the genitourinary tract, such as the ureter and urethra, are challenging because of their complexity and special milieu that gives rise to incrustation, inflammation and stricture formation...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28733605/genexpert-mtb-rif-assay-in-the-diagnosis-of-urinary-tuberculosis-from-urine-specimens
#12
Yu Pang, Yuanyuan Shang, Jie Lu, Qian Liang, Lingling Dong, Yunxu Li, Liping Zhao, Guanglu Jiang, Hairong Huang
Conventional bacteriological methods are not generally helpful in diagnosing urinary tuberculosis (UTB). GeneXpert is endorsed for the detection of pulmonary tuberculosis, whereas the data on its utility for urine specimens is limited. In this study, we aimed to evaluate its performance on urine specimens in a country with high TB incidence. A total of 163 suspected UTB patients were consecutively enrolled in the analysis, including 37 (22.7%) culture-positive and 44 (27.0%) clinically diagnosed UTB cases. Compared with conventional culture, the sensitivity of GeneXpert (94...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28728558/accuracy-of-a-plasmodium-falciparum-specific-histidine-rich-protein-2-rapid-diagnostic-test-in-the-context-of-the-presence-of-non-malaria-fevers-prior-anti-malarial-use-and-seasonal-malaria-transmission
#13
Francois Kiemde, Massa Dit Achille Bonko, Marc Christian Tahita, Palpouguini Lompo, Toussaint Rouamba, Halidou Tinto, Michael Boele van Hensbroek, Petra F Mens, Henk D F H Schallig
BACKGROUND: It remains challenging to distinguish malaria from other fever causing infections, as a positive rapid diagnostic test does not always signify a true active malaria infection. This study was designed to determine the influence of other causes of fever, prior anti-malarial treatment, and a possible seasonality of the performance of a PfHRP2 RDT for the diagnosis of malaria in children under-5 years of age living in a malaria endemic area. METHODS: A prospective etiology study was conducted in 2015 among febrile children under 5 years of age in Burkina Faso...
July 20, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28716013/long-term-renal-survival-of-%C3%AE-3-heavy-chain-deposition-disease-a-case-report
#14
Takayuki Katsuno, Shige Mizuno, Masatsuna Mabuchi, Naotake Tsuboi, Atsushi Komatsuda, Shoichi Maruyama
BACKGROUND: Monoclonal immunoglobulin deposition disease (MIDD) is characterized by the non-amyloid deposition of monoclonal immunoglobulin fragments in the basement membranes. Heavy chain deposition disease (HCDD) is a type of MIDD. HCDD is an extremely rare disease, and only three cases have been reported in Japan up to the present. The prognosis of HCDD is very poor, and optimal treatment has not been established. Only a few cases of HCDD with favorable long-term renal prognosis have been reported to date...
July 17, 2017: BMC Nephrology
https://www.readbyqxmd.com/read/28711277/use-of-a-midstream-clean-catch-mobile-application-did-not-lower-urine-contamination-rates-in-an-emergency-department
#15
Mary S Jacob, Paige Kulie, Cameron Benedict, Alexander J Ordoobadi, Neal Sikka, Erika Steinmetz, Melissa L McCarthy
OBJECTIVES: Urine microscopy is a common test performed in emergency departments (EDs). Urine specimens can easily become contaminated by different factors, including the collection method. The midstream clean-catch (MSCC) collection technique is commonly used to reduce urine contamination. The urine culture contamination rate from specimens collected in our ED is 30%. We developed an instructional application (app) to show ED patients how to provide a MSCC urine sample. We hypothesized that ED patients who viewed our instructional app would have significantly lower urine contamination rates compared to patients who did not...
July 8, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28693436/urinary-schistosomiasis-among-vulnerable-children-in-a-rehabilitation-home-in-ibadan-oyo-state-nigeria
#16
Obioma Uchendu, Victoria Oladoyin, Michael Idowu, Oluwapelumi Adeyera, Oluwatosin Olabisi, Oluwafisayomi Oluwatosin, Gbemisola Leigh
BACKGROUND: Schistosomiasis is a disease of public health importance with long term complications mostly common among children, rural dwellers, poor and migrant workers. Studies have not documented the burden among migrant workers and their families. The study aimed to describe the burden of schistosomiasis and demographic characteristics among children of migrant workers residing in a rehabilitation home in Ibadan, Nigeria. METHODS: A cross-sectional study using sixty six children, who were tested following complaints of haematuria by six of them...
July 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28685832/assessment-of-genotoxicity-and-biodistribution-of-nano-and-micron-sized-yttrium-oxide-in-rats-after-acute-oral-treatment
#17
Archana Panyala, Srinivas Chinde, Srinivas Indu Kumari, Paramjit Grover
The increasing use of yttrium oxide (Y2 O3 ) nanoparticles (NPs) entails an improved understanding of their potential impact on the environmental and human health. In the present study, the acute oral toxicity of Y2 O3 NPs and their microparticles (MPs) was carried out in female albino Wistar rats with 250, 500 and 1000 mg kg(-1) body weight doses. Before the genotoxicity evaluation, characterization of the particles by transmission electron microscopy, dynamic light scattering and laser Doppler velocimetry was performed...
July 7, 2017: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/28668772/field-effect-transition-sensor-for-ki-detection-based-on-self-assembled-calixtube-monolayers
#18
Kirill Puchnin, Mariia Andrianova, Alexander Kuznetsov, Vladimir Kovalev
A series of novel calixarene-based tubes comprising different numbers of silatrane anchoring groups was synthesized. For the first time, a self-assembled monolayer (SAM) derived from calixtubes was formed on a SiO2 surface. The formation of the SAM was confirmed by X-ray photoelectron spectroscopy, scanning electron microscopy-energy dispersive X-ray analysis, and contact angle measurements. Modification of the sensitive surface of a conventional ion-selective field effect transistor (ISFET) with the afforded SAM resulted in the production of a KI-sensitive sensor...
June 28, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28659153/advanced-glycation-end-products-ages-increase-renal-lipid-accumulation-a-pathogenic-factor-of-diabetic-nephropathy-dn
#19
Yang Yuan, Hong Sun, Zilin Sun
BACKGROUND: Advanced glycation end products (AGEs) are pathogenic factors of diabetic nephropathy (DN), causing renal damage in various ways. The aim of this study is to investigate the ectopic lipid accumulation caused by AGEs in human renal tubular epithelial cell line (HK-2) cells and the kidney of type 2 diabetic rats. METHODS: In vivo study, diabetes was induced in male Sprague-Dawley rats through intraperitoneal injection of high-fat/high-sucrose diet and low-dose streptozocin (STZ)...
June 28, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28658768/clinicopathological-analysis-of-glomerular-disease-of-adult-onset-nephrotic-syndrome-in-an-indian-cohort-a-retrospective-study
#20
Mayur Suryawanshi, Swapnil Karnik, Sanjeet Roy
INTRODUCTION: Primary glomerular disease presenting with adult onset nephrotic syndrome are a major cause of chronic renal failure worldwide. The spectrum of renal disease presenting with nephrotic syndrome has undergone a gradual change globally over the course of time. However, there still exist regional differences in the incidence of primary glomerular diseases causing adult onset nephrotic syndrome. AIM: To observe the spectrum of renal diseases presenting with adult onset nephrotic syndrome with comparative analysis of changing trends over the last five decades with regards to Western and Indian literature...
May 2017: Journal of Clinical and Diagnostic Research: JCDR
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