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Urine microscopy

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https://www.readbyqxmd.com/read/29225801/histologic-regression-of-fibrillary-glomerulonephritis-the-first-report-of-biopsy-proven-spontaneous-resolution-of-disease
#1
Miroslav Sekulic, Samih H Nasr, Joseph P Grande, Lynn D Cornell
Fibrillary glomerulonephritis (FGN) is a rare immune complex type glomerulonephritis characterized by glomerular deposition of randomly oriented fibrils measuring 10-30 nm in thickness, and typically presents with proteinuria with or without renal insufficiency and hematuria. We present a case in which a patient initially presented at age 41 years with nephrotic-range proteinuria and hypertension; a kidney biopsy showed FGN. The patient was treated with angiotensin receptor blockage only, without immunosuppression as per patient preference, and the level of protein in the urine improved...
December 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/29221467/urothelial-cells-may-indicate-underlying-bacteriuria-in-pregnancy-at-term-a-comparative-study
#2
N Liou, J Currie, C James, J Malone-Lee, A L David
BACKGROUND: Urinary tract infection is common in pregnancy. Urine is sampled from by mid-stream collection (MSU). If epithelial cells are detected, contamination by vulvo-vagial skin and skin bacteria is assumed. Outside pregnancy, catheter specimen urine (CSU) is considered less susceptible to contamination. We compared MSU and CSU methods in term pregnancy to test these assumptions. METHODS: Healthy pregnant women at term gestation (n = 32, median gestation 38 + 6 weeks, IQR 37 + 6-39 + 2) undergoing elective caesarean section provided a MSU and CSU for paired comparison that were each analysed for bacterial growth and bladder distress by fresh microscopy, sediment culture and immunofluorescent staining...
December 8, 2017: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/29215338/evaluation-of-antimicrobial-durability-and-anti-biofilm-effects-in-urinary-catheters-against-enterococcus-faecalis-clinical-isolates-and-reference-strains
#3
Didem Kart, Ayşe Semra Kustimur, Meral Sağıroğlu, Ayşe Kalkancı
BACKGROUND: Enterococcus faecalis, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa and Candida albicans biofilms are major causes of catheter-associated urinary tract infections. Antimicrobial-coated or impregnated urinary catheters are seen as a possible way to prevent these infections. AIMS: To determine the biofilm-forming ability of 89 E. faecalis isolates from urinary tract infections and to compare several urinary catheters for antimicrobial durability and the inhibitory effects on biofilm formation of different laboratory strains and clinical isolates of E...
December 1, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/29203238/targeting-erythropoietin-protects-against-proteinuria-in-type-2-diabetic-patients-and-in-zebrafish
#4
Jianqing She, Zuyi Yuan, Yue Wu, Junfang Chen, Jens Kroll
OBJECTIVE: Adult human kidneys produce erythropoietin (EPO), which regulates red blood cell formation; however, whether EPO also functions directly on kidney development and controls diabetic kidney disease remains unknown. Here we analyzed the role of EPO in kidney development and under hyperglycemic conditions in zebrafish and in humans. METHODS: Diabetic patients and respective controls were enrolled in two cohorts. Serum EPO level and urine protein change upon human EPO administration were then analyzed...
November 22, 2017: Molecular Metabolism
https://www.readbyqxmd.com/read/29184111/non-invasive-and-invasive-diagnoses-of-aspergillosis-in-a-rat-model-by-mass-spectrometry
#5
Dominika Luptáková, Tomáš Pluháček, Miloš Petřík, Jiří Novák, Andrea Palyzová, Lucie Sokolová, Anton Škríba, Blanka Šedivá, Karel Lemr, Vladimír Havlíček
Invasive pulmonary aspergillosis results in 450,000 deaths per year and complicates cancer chemotherapy, transplantations and the treatment of other immunosuppressed patients. Using a rat model of experimental aspergillosis, the fungal siderophores ferricrocin and triacetylfusarinine C were identified as markers of aspergillosis and quantified in urine, serum and lung tissues. Biomarkers were analyzed by matrix-assisted laser desorption ionization (MALDI) and electrospray ionization mass spectrometry using a 12T SolariX Fourier transform ion cyclotron resonance (FTICR) mass spectrometer...
November 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29181309/effect-of-mistletoe-combined-with-carboxymethyl-cellulose-on-dry-eye-in-postmenopausal-women
#6
Nan Jiang, Lin-Hong Ye, Lei Ye, Jing Yu, Qi-Chen Yang, Qing Yuan, Pei-Wen Zhu, Yi Shao
AIM: To investigate the protective effect of mistletoe combined with carboxymethyl cellulose eye drops on dry eye in postmenopausal women. METHODS: Sixty postmenopause female patients diagnosed of dry eye were assigned randomly to mistletoe combined with carboxymethyl cellulose eye drops treatment group (n=30) and control group treated with normal saline eye drops (n=30). The subjective symptoms of ocular surface, Ocular Surface Disease Index (OSDI), tear film function tests, tear protein and corneal morphology by confocal scanning microscopy were analyzed before treatment and at 1, 2, 4 and 8wk after treatment respectively...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29181028/comparison-of-the-performance-of-urinary-mycobacterium-tuberculosis-antigens-cocktail-esat6-cfp10-and-mpt64-with-culture-and-microscopy-in-pulmonary-tuberculosis-patients
#7
Dewi Kartika Turbawaty, Adhi Kristianto Sugianli, Arto Yuwono Soeroto, Budi Setiabudiawan, Ida Parwati
Pulmonary tuberculosis (TB) is a major global health problem and is one of the top 10 causes of death worldwide. Our study aimed to evaluate the performance of urinary Mycobacterium tuberculosis (Mtb) antigens cocktail (ESAT6, CFP10, and MPT64) compared with culture and microscopy. This descriptive cross-sectional study was conducted in Dr. Hasan Sadikin General Hospital, Bandung, from January 2014 to October 2016. A total of 141 pulmonary tuberculosis patients were included. Sputum samples were examined for acid-fast bacilli (ZN stain) and mycobacterial culture (LJ); the Mtb antigens cocktail was examined in the urine sample...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29178268/selective-extraction-of-morphine-from-biological-fluids-by-magnetic-molecularly-imprinted-polymers-and-determination-using-uhplc-with-diode-array-detection
#8
Mahdiyeh Ebrahimi Rahmani, Mehdi Ansari, Maryam Kazemipour, Mohammadreza Nateghi
The determination of morphine concentration in the blood and urine is necessary for patients and recruitment purposes. Herein, a magnetic molecularly imprinted polymer for selective and efficient extraction of morphine from biological samples was synthesized by using a core-shell method. Fe3 O4 nanoparticles were coated with SiO2 -NH2 . The molecularly imprinted polymer was coated on the Fe3 O4 /SiO2 -NH2 surface by the copolymerization of methacrylic acid and ethylene glycol dimethacrylate in the presence of morphine as the template molecule...
November 27, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29169771/antiurolithiatic-activity-of-natural-constituents-isolated-from-aerva-lanata
#9
Basavaraj M Dinnimath, Sunil S Jalalpure, Umesh K Patil
BACKGROUND: Pashanabheda is used as antiurolithiatic in Ayurveda. In the present study, Aerva lanata (L) Juss. ex. Schult (Amaranthaceae) from Western Ghats of India was selected for isolation of active constituents and screening for antiurolithiatic potentials. OBJECTIVE: Screening of compounds isolated from A. lanata for antiurolithiatic potentials. MATERIALS AND METHODS: Ethylene glycol (0.75% v/v) induced urolithiasis model was used to study the antiurolithiatic activity in male Wistar albino rats...
November 20, 2017: Journal of Ayurveda and Integrative Medicine
https://www.readbyqxmd.com/read/29163438/culture-strategies-for-isolation-of-fastidious-leptospira-serovar-hardjo-and-molecular-differentiation-of-genotypes-hardjobovis-and-hardjoprajitno
#10
Roberta T Chideroli, Daniela D Gonçalves, Suelen A Suphoronski, Alice F Alfieri, Amauri A Alfieri, Admilton G de Oliveira, Julio C de Freitas, Ulisses de Padua Pereira
The Leptospira serovar Hedjo belongs to the serogroup sejroe and this serovar is the most prevalent in bovine herds worldwide. The sejroe serogroup is the most frequently detected by serology in Brazilian cattle herds suggesting that it is due serovar Hardjo. In the molecular classification, this serovar has two genotypes: Hardjobovis and Hardjoprajitno. This serovar is as considered as fastidious pathogens, and their isolation is one of the bottlenecks in leptospirosis laboratories. In addition, its molecular characterization using genomic approaches is oftentimes not simple and time-consuming...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29156008/urinalysis-for-the-diagnosis-of-glomerulonephritis-role-of-dysmorphic-red-blood-cells
#11
Abdurrahman M Hamadah, Kamel Gharaibeh, Kristin C Mara, Katherine A Thompson, John C Lieske, Samar Said, Samih H Nasr, Nelson Leung
Background: Dysmorphic red blood cells (dRBCs) on urine microscopy have been associated with glomerulonephritis (GN). We assessed the prevalence and ability of dRBCs to differentiate GN from other kidney diseases. Methods: Adult patients with kidney biopsy performed between 2012 and 2015 at a single center who had a concurrent urinalysis were retrospectively studied. The association of ≥25% dRBCs with the presence of glomerular pathology was assessed. Univariate and multivariate logistic regression were performed on significantly associated variables...
October 6, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29151005/synthesis-of-a-novel-polymeric-magnetic-solid-phase-extraction-adsorbent-for-selective-extraction-of-amphetamine-from-urine-samples-coupled-with-high-performance-liquid-chromatography
#12
Arezou Taghvimi, Marjan Ghorbani, Hamed Hamishehkar
A novel pH-responsive block copolymer (Poly ethylene glycol-b-poly (N,N-dimethylaminoethylmethacrylate-co-maleic acid) was designed for the decoration and stabilization of magnetic nanoparticles (MNPs) to apply as an efficient magnetic nano adsorbent for extraction of amphetamine (AM) from biological urine samples to be determined by high performance liquid chromatography ultra violet detector (HPLC-UV). Full characterization of the synthesized Polymeric magnetic nanoparticles (PMNPs) were followed by various techniques like Fourier transform infrared (FT-IR) spectroscopy, powder x-ray diffraction (XRD), scanning electron microscopy (SEM), and vibrating sample magnetometer (VSM)...
November 18, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29145491/the-urine-biomarker-panel-igfbp7-x-timp-2-nephrocheck%C3%A2-parameter-does-not-correlate-with-igfbp7-and-timp-2-gene-expression-in-urinary-sediment
#13
Daniela Knafl, Markus Müller, Sahra Pajenda, Zeynep Genc, Manfred Hecking, Ludwig Wagner
BACKGROUND: Acute kidney injury (AKI) is frequently observed in serious infections, following nephrotoxic medication, surgery and trauma. Here we tested whether the detection of two recently identified biomarkers for AKI, Tissue Inhibitor of Metalloproteinase-2 (TIMP-2) and Insulin-Like Growth Factor Binding Protein 7 (IGFBP7), depends on the expression of these proteins in cells of the urinary sediment. METHOD: We collected urine samples of 33 kidney transplant recipients and 14 non-transplanted patients who all had AKI (stages 1-3 according to KDIGO), and measured [IGFBP7]x[TIMP-2] using the NephroCheck® Astute1 40 ™ meter...
2017: PloS One
https://www.readbyqxmd.com/read/29142988/proximal-tubular-expression-patterns-of-megalin-and-cubilin-in-proteinuric-nephropathies
#14
Jia Sun, Kjell Hultenby, Jonas Axelsson, Johan Nordström, Bing He, Annika Wernerson, Karin Lindström
Introduction: Receptor-mediated endocytosis is responsible for protein reabsorption in the proximal tubules. For albumin this process involves at least 2 interacting receptors, megalin and cubilin. Albumin is not usually present in the urine, indicating a highly efficient tubular reuptake under physiological conditions. However, early appearance of albuminuria may mean that the tubular system is overwhelmed by large quantities of albumin or that the function is impaired. Methods: To better understand the physiological role of megalin and cubilin in human renal disease, renal biopsies from 15 patients with a range of albuminuria and 3 healthy living donors were analyzed for proximal tubular expression of megalin and cubilin using immunohistochemistry (IHC) and semiquantitative immune-electron microscopy...
July 2017: KI Reports
https://www.readbyqxmd.com/read/29141749/visualization-of-west-nile-virus-in-urine-sediment-using-electron-microscopy-and-immunogold-up-to-nine-years-postinfection
#15
Kristy O Murray, Steven Kolodziej, Shannon E Ronca, Rodion Gorchakov, Patricia Navarro, Melissa S Nolan, Amber Podoll, Kevin Finkel, Sreedhar Mandayam
West Nile virus (WNV) is an important emerging flavivirus in North America. Experimental studies in animals infer the development of persistent infection in the kidneys. In humans, recent studies suggest the possibility of persistent renal infection and chronic kidney disease. Considering the discrepancies between published studies on viral RNA detection in urine of convalescing WNV-positive patients, we explored the use of electron microscopy (EM) with anti-WNV E protein antibody immunogold labeling to detect virus in the urine sediment from a subset of study participants in the Houston WNV cohort...
October 23, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29137703/fabrication-of-a-novel-aptasensor-based-on-three-dimensional-reduced-graphene-oxide-polyaniline-gold-nanoparticle-composite-as-a-novel-platform-for-high-sensitive-and-specific-cocaine-detection
#16
Pegah Hashemi, Hasan Bagheri, Abbas Afkhami, Yalda Hosseinzadeh Ardakani, Tayyebeh Madrakian
In the present research, we have developed a novel label free aptasensor based on screen printed carbon electrode (SPCE) modified with three-dimensional magnetic reduced graphene oxide(3D-MRGO)/polyaniline(PA)/gold nanoparticle(AuNP) nanocomposite for impedimetric determination of cocaine. To achieve this goal, a specific thiolated cocaine aptamer was immobilized onto the surface of synthesized nanocomposite. The signaling mechanism of the proposed aptasensor was based on increase in the [Fe(CN)6]3-/4- charge transfer resistance (RCT) as an electrochemical probe in the presence of target analyte...
December 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29136947/the-effect-of-the-dipeptidyl-peptidase-4-inhibitor-sitagliptin-on-gentamicin-nephrotoxicity-in-mice
#17
Yousuf M Al Suleimani, Aly M Abdelrahman, Turan Karaca, Priyadarsini Manoj, Mohammed Ashique, Abderrahim Nemmar, Badreldin H Ali
This study aimed at investigating the possible ameliorative effects of sitagliptin in mice with gentamicin (GEN) nephrotoxicity. Sitagliptin was given to the animals at an oral dose of 10mgkg-1 per day for 10days, and in some of these mice, GEN was injected intraperitoneally at a dose of 100mgkg-1 per day during the last seven days of the treatment. Nephrotoxicity was evaluated histopathologically by light microscopy and biochemically by measuring several indices in plasma, urine and renal cortex homogenates...
November 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29136792/comparative-performances-of-a-bare-graphite-polyurethane-composite-electrode-unmodified-and-modified-with-graphene-and-carbon-nanotubes-in-the-electrochemical-determination-of-escitalopram
#18
Marina Baccarin, Priscila Cervini, Eder Tadeu Gomes Cavalheiro
A bare composite graphite-polyurethane electrode (EGPU) and two other modified with graphene (EGPU-GR) and functionalized multi-walled carbon nanotubes (EGPU-CNTs) were prepared and compared regarding their voltammetric response to escitalopran (EST). The modifiers were characterized by Raman spectroscopy and the resulting electrode materials by contact angle measurements with a hydrophilicity character in the ascending order for the composites: GPU > GPU-GR > GPU-CNTs and scanning electron microscopy (SEM)...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29128151/is-the-choosing-wisely-%C3%A2-campaign-model-applicable-to-the-management-of-multiple-sclerosis-in-france-a-gresep-pilot-study
#19
B Trumbic, H Zéphir, J-C Ouallet, E Le Page, D Laplaud, C Bensa, J de Sèze
BACKGROUND: Launched in the US in 2012, Choosing Wisely(®) is a campaign promoted by the American Board of Internal Medicine (ABIM) Foundation with the goal of improving healthcare effectiveness by avoiding wasteful or unnecessary medical tests, treatments and procedures. It uses concise recommendations produced by national medical societies to start discussions between physicians and patients on the relevance of these services as part of a shared decision-making process. The Multiple Sclerosis Focus Group (Groupe de Reflexion Autour de la Sclérose en Plaques; GRESEP) undertook a pilot study to assess the relevance and feasibility of this approach in the management of multiple sclerosis (MS) in France...
November 8, 2017: Revue Neurologique
https://www.readbyqxmd.com/read/29121996/production-and-characterization-of-monoclonal-antibodies-against-encephalitozoon-intestinalis-and-encephalitozoon-sp-spores-and-their-developmental-stages
#20
Fernando Izquierdo, Hercules Moura, Fernando Jorge Bornay-Llinares, Rama Sriram, Carolina Hurtado, Ángela Magnet, Soledad Fenoy, Govinda Visvesvara, Carmen Del Aguila
BACKGROUND: Microsporidia are intracellular obligate parasites traditionally associated with immunosuppressed patients; their detection in immunocompetent patients has increased, highlighting their possible importance as emerging pathogens. Detection of spores in stools, urine, body fluids and tissues is difficult and immunological techniques such as immunofluorescence have proved to be a useful and reliable tool in the diagnosis of human microsporidiosis. For this reason, we have produced and characterized monoclonal antibodies (MAbs) specific for Encephalitozoon intestinalis (the second most frequent microsporidian infecting humans), and other Encephalitozoon species, that can be used in different diagnostic techniques...
November 9, 2017: Parasites & Vectors
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