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Qi zhou CAS

Cheng-Zhou Mao, Li Zheng, Yi-Min Zhou, Hai-Yan Wu, Jing-Bo Xia, Chi-Qian Liang, Xiao-Fang Guo, Wen-Tao Peng, Hui Zhao, Wei-Bin Cai, Soo-Ki Kim, Kyu-Sang Park, Dong-Qing Cai, Xu-Feng Qi
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 system has emerged as a powerful tool for knock-in of DNA fragments via donor plasmid and homology-independent DNA repair mechanism; however, conventional integration includes unnecessary plasmid backbone and may result in the unfaithful expression of the modified endogenous genes. Here, we report an efficient and precise CRISPR/Cas9-mediated integration strategy using a donor plasmid that harbors 2 of the same cleavage sites that flank the cassette at both sides...
June 13, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Di Liu, Zhongyao Zhao, Anxin Wang, Siqi Ge, Hao Wang, Xiaoyu Zhang, Qi Sun, Weijie Cao, Ming Sun, Lijuan Wu, Manshu Song, Yong Zhou, Wei Wang, Youxin Wang
BACKGROUND: Glycosylation significantly affects protein structure and function and thus participates in multiple physiologic and pathologic processes. Studies demonstrated that immunoglobulin G (IgG) N-glycosylation associates with the risk factors of ischemic stroke (IS), such as aging, obesity, dyslipidemia, type 2 diabetes, and hypertension. METHODS: The study aimed to investigate the association between IgG N-glycosylation and IS in a Chinese population. IgG glycome composition in patients with IS (n = 78) and cerebral arterial stenosis (CAS) (n = 75) and controls (n = 77) were analyzed by ultra-performance liquid chromatography...
April 26, 2018: Journal of Neuroinflammation
Yuxuan Wu, Hai Zhou, Xiaoying Fan, Ying Zhang, Man Zhang, Yinghua Wang, Zhenfei Xie, Meizhu Bai, Qi Yin, Dan Liang, Wei Tang, Jiaoyang Liao, Chikai Zhou, Wujuan Liu, Ping Zhu, Hongshan Guo, Hong Pan, Chunlian Wu, Huijuan Shi, Ligang Wu, Fuchou Tang, Jinsong Li
Spermatogonial stem cells (SSCs) can produce numerous male gametes after transplantation into recipient testes, presenting a valuable approach for gene therapy and continuous production of gene-modified animals. However, successful genetic manipulation of SSCs has been limited, partially due to complexity and low efficiency of currently available genetic editing techniques. Here, we show that efficient genetic modifications can be introduced into SSCs using the CRISPR-Cas9 system. We used the CRISPR-Cas9 system to mutate an EGFP transgene or the endogenous Crygc gene in SCCs...
January 2015: Cell Research
Qi Wang, Ming Zhang, Dian Huang, Yu Zhou, Tong Qiao
OBJECTIVE: To investigate the theraputic effects of carotid endarterectomy (CEA) on cognitive function in patients with carotid stenosis (CAS) and congnitive impairment. METHODS: CEA was performed on 38 patients with CAS from December 2011 to July 2013. There were 26 male and 12 female patients, with an average age of (70 ± 7) years. Patients was underwent neuropsychological examinations (NPEs) including Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA) at 1 week before and 6 weeks after treatment...
August 2014: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
Xue Li, Chang-peng Xu, Yi-long Hou, Jin-qi Song, Zhuang Cui, Sheng-nan Wang, Lei Huang, Chang-ren Zhou, Bin Yu
Distraction osteogenesis after aggrieved bone segment resections is promising in the treatment of bone tumors and osteomyelitis. However, there is ambiguity with regard to the optimal choice of bone substitute, with biodegradability and excellent bone repair performance constituting key requirements. The purpose of this study was to develop a novel resorbable strontium-containing α-calcium sulfate hemihydrate (Sr-CaS) bone substitute to provide an alternative option for surgeons that better meets these requirements...
August 2014: Biomedical Materials
Xue Guo, Huaqun Yin, Yili Liang, Qi Hu, Xishu Zhou, Yunhua Xiao, Liyuan Ma, Xian Zhang, Guanzhou Qiu, Xueduan Liu
The genus Sulfobacillus is a cohort of mildly thermophilic or thermotolerant acidophiles within the phylum Firmicutes and requires extremely acidic environments and hypersalinity for optimal growth. However, our understanding of them is still preliminary partly because few genome sequences are available. Here, the draft genome of Sulfobacillus thermosulfidooxidans strain ST was deciphered to obtain a comprehensive insight into the genetic content and to understand the cellular mechanisms necessary for its survival...
2014: PloS One
LinLin Zhang, Qi Zhou
No abstract text is available yet for this article.
June 2014: Science China. Life Sciences
Wei Li, Xin Li, Tianda Li, Ming-Gui Jiang, Haifeng Wan, Guan-Zheng Luo, Chunjing Feng, Xiaolong Cui, Fei Teng, Yan Yuan, Quan Zhou, Qi Gu, Ling Shuai, Jiahao Sha, Yamei Xiao, Liu Wang, Zhonghua Liu, Xiu-Jie Wang, Xiao-Yang Zhao, Qi Zhou
The rat is an important animal model in biomedical research, but practical limitations to genetic manipulation have restricted the application of genetic analysis. Here we report the derivation of rat androgenetic haploid embryonic stem cells (RahESCs) as a tool to facilitate such studies. Our approach is based on removal of the maternal pronucleus from zygotes to generate androgenetic embryos followed by derivation of ESCs. The resulting RahESCs have 21 chromosomes, express pluripotency markers, differentiate into three germ layer cells, and contribute to the germline...
March 6, 2014: Cell Stem Cell
Yan Wang, Lei Ji, Bingbo Zhang, Peihao Yin, Yanyan Qiu, Daqian Song, Juying Zhou, Qi Li
Multi-modal imaging based on multifunctional nanoparticles is a promising alternative approach to improve the sensitivity of early cancer diagnosis. In this study, highly upconverting fluorescence and strong relaxivity rare-earth nanoparticles coated with paramagnetic lanthanide complex shells and polyethylene glycol (PEGylated UCNPs@DTPA-Gd(3+)) are synthesized as dual-modality imaging contrast agents (CAs) for upconverting fluorescent and magnetic resonance dual-modality imaging. PEGylated UCNPs@DTPA-Gd(3+) with sizes in the range of 32-86 nm are colloidally stable...
May 3, 2013: Nanotechnology
Jie Wu, You-Bin Wu, Dong-Xia Hao, Meng Zhou, Qing-Zhe Fan, Yue-Qi Wang, Guang-Hui Ma
The National Key Laboratory of Biochemical Engineering established in 1995 is affiliated with the Institute of Process Engineering, Chinese Academy of Sciences (CAS), and located in Zhong-guan-cun (Beijing, China). The National Key Laboratory of Biochemical Engineering is working towards developments in the fields of bio-reaction, bioseparation and bio-formulation, by chemical and material methods. Over the last 5 years, approximately 200 scientists and students have worked at the laboratory, and published over 400 articles...
October 2012: Therapeutic Delivery
Shun-bao Lu, Xiao-qi Zhou, Yi-chao Rui, Cheng-rong Chen, Zhi-hong Xu, Xiao-min Guo
Taking the typical forest types Pinus elliottii var. elliotttii, Araucaria cunninghamii, and Agathis australis in southern Queensland of Australia as test objects, an investigation was made on the soil soluble organic carbon (SOC) and nitrogen (SON), microbial biomass C (MBC) and N (MBN), and enzyme activities, aimed to understand the effects of forest type on soil quality. In the three forests, soil SOC content was 552-1154 mg kg(-1), soil SON content was 20.11-57.32 mg kg(-1), soil MBC was 42-149 mg kg(-1), soil MBN was 7-35 mg kg(-1), soil chitinase (CAS) activity was 2...
October 2011: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
Yi-Mei Wang, Shuang-Qing Peng, Qi Zhou, Min-Wei Wang, Chang-Hui Yan, Guo-Qiang Wang, Hai-Ying Yang
Butenolide (CAS No. 16275-44-8), a mycotoxin produced by several Fusarium species, has been shown to be a potential risk factor for animal and human health. This study was undertaken to investigate the potential oxidative damage of butenolide to biomembranes in vitro using the erythrocyte membrane model. Following exposure of isolated rat erythrocyte membranes to butenolide, the extent of oxidative damage was assessed by measuring lipid peroxidation, -SH groups content, Ca2+/Mg2+-ATPase and Na+/K+-ATPase activities, and conformational changes in membrane proteins...
August 2007: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Kai-song Zhang, Qi-xing Zhou, Hong Xiao
Combined flocculants with low ecological risk are urgently required in water supply and wastewater treatment in China. A novel flocculant was thus developed under the condition of low ecological risk (noted as CAS). The experiments to examine wastewater treatment performance of the new product showed that there was favourable performance in the flocculation process in contrast to commercial flocculants in treating kaolin suspensions, municipal effluent and domestic wastewater. Flocculation performance included the turbidity removal rate, sediment character and a decrease in COD (chemical oxygen demand)...
2004: Journal of Environmental Sciences (China)
Qi-Shui Lin, Bo-Liang Li
Professor WANG Ying-Lai, world-renowned biochemist and a member of the Chinese Academy of Sciences, passed away at 19:10, May 5, 2001 resulting from an incurable disease. Since then, the Chinese Communist Party has lost an outstanding member struggling all his life for the development of science in China. The scientific communities in China have lost an outstanding scientist enjoying world prestige. His colleagues and students have lost a valuable friend and good teacher. Today, although he has left us, his scientific achievement and noble morality will be engraved on our mind forever...
2001: Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica et Biophysica Sinica
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