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Theresa A Niklas

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https://www.readbyqxmd.com/read/26930550/an-antigen-specific-four-color-b-cell-fluorospot-assay-utilizing-tagged-antigens-for-detection
#1
Peter Jahnmatz, Theresa Bengtsson, Bartek Zuber, Anna Färnert, Niklas Ahlborg
The FluoroSpot assay, a variant of ELISpot utilizing fluorescent detection, has so far been used primarily for assessment of T cells, where simultaneous detection of several cytokines has allowed a more qualitative analysis of functionally distinct T cells. The potential to measure multiple analytes also presents several advantages when analyzing B cells. Our aim was to develop a B-cell FluoroSpot assay adaptable to studies of a variety of antigens. The assay utilizes anti-IgG antibodies immobilized in 96-well filter membrane plates...
June 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/26683397/multicenter-validation-of-the-quality-of-trauma-care-patient-reported-experience-measure-qtac-prem
#2
MULTICENTER STUDY
Niklas Bobrovitz, Maria J Santana, Theresa Kline, John Kortbeek, Sandy Widder, Kevin Martin, Henry T Stelfox
BACKGROUND: Incorporating patient and family perspectives into injury care quality assessment is a necessary part of comprehensive quality improvement. However, tools to measure patient and family perspectives of injury care are lacking. Therefore, our objective was to assess the psychometric properties of the Quality of Trauma Care Patient-Reported Experience Measure (QTAC-PREM), the first measure developed to assess patient experiences with overall injury care. METHODS: We conducted a prospective multicenter cohort study of adult injury patients recruited from three trauma centers...
January 2016: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/25589232/the-use-of-cognitive-interviews-to-revise-the-quality-of-trauma-care-patient-reported-experience-measure-qtac-prem
#3
Niklas Bobrovitz, Maria J Santana, Theresa Kline, John Kortbeek, Henry T Stelfox
PURPOSE: The "Quality of Trauma Care Patient-Reported Experience Measure" is the first measure of patient experiences with overall injury care. The objective of this study was to use cognitive interviews to inform revision of the measure into a parsimonious set of items that function as intended, in preparation for multicenter testing. METHODS: Concurrent and retrospective cognitive interviews with injured patients (n = 17) and family members (n = 13) using semi-structured interview guides...
August 2015: Quality of Life Research
https://www.readbyqxmd.com/read/25382658/immature-dendritic-cells-convert-anergic-nonregulatory-t-cells-into-foxp3-il-10-regulatory-t-cells-by-engaging-cd28-and-ctla-4
#4
Katrien Pletinckx, Martin Vaeth, Theresa Schneider, Niklas Beyersdorf, Thomas Hünig, Friederike Berberich-Siebelt, Manfred B Lutz
Anergic T cells can survive for long time periods passively in a hyporesponsive state without obvious active functions. Thus, the immunological reason for their maintenance is unclear. Here, we induced peptide-specific anergy in T cells from mice by coculturing these cells with immature murine dendritic cells (DCs). We found that these anergic, nonsuppressive IL-10(-) Foxp3(-) CTLA-4(+) CD25(low) Egr2(+) T cells could be converted into suppressive IL-10(+) Foxp3(-) CTLA-4(+) CD25(high) Egr2(+) cells resembling type-1 Treg cells (Tr1) when stimulated a second time by immature DCs in vitro...
February 2015: European Journal of Immunology
https://www.readbyqxmd.com/read/23496959/prospective-cohort-study-protocol-to-evaluate-the-validity-and-reliability-of-the-quality-of-trauma-care-patient-reported-experience-measure-qtac-prem
#5
Niklas Bobrovitz, Maria Santana, Theresa Kline, John Kortbeek, Henry T Stelfox
BACKGROUND: Patient-centeredness is a key component of health care quality. However, patient-centered measures of quality have not been developed in injury care. In response to this challenge, we developed the Quality of Trauma Adult Care Patient-Reported Experience Measure (QTAC-PREM) to measure injured patient experiences with trauma care and pilot-tested the instrument at a single Level 1 trauma centre. The objective of this study is to test the reliability, validity, and feasibility of the QTAC-PREM in multiple Canadian trauma centers and to refine the measure based on the results...
2013: BMC Health Services Research
https://www.readbyqxmd.com/read/22917143/reference-measurement-procedures-for-alzheimer-s-disease-cerebrospinal-fluid-biomarkers-definitions-and-approaches-with-focus-on-amyloid-%C3%AE-42
#6
REVIEW
Niklas Mattsson, Ingrid Zegers, Ulf Andreasson, Maria Bjerke, Marinus A Blankenstein, Robert Bowser, Maria C Carrillo, Johan Gobom, Theresa Heath, Rand Jenkins, Andreas Jeromin, June Kaplow, Daniel Kidd, Omar F Laterza, Andrew Lockhart, Michael P Lunn, Robert L Martone, Kevin Mills, Josef Pannee, Marianne Ratcliffe, Leslie M Shaw, Adam J Simon, Holly Soares, Charlotte E Teunissen, Marcel M Verbeek, Robert M Umek, Hugo Vanderstichele, Henrik Zetterberg, Kaj Blennow, Erik Portelius
Cerebrospinal fluid (CSF) biomarkers for Alzheimer's disease (AD) are increasingly used in clinical settings, research and drug trials. However, their broad-scale use on different technology platforms is hampered by the lack of standardization at the level of sample handling, determination of concentrations of analytes and the absence of well-defined performance criteria for in vitro diagnostic or companion diagnostic assays, which influences the apparent concentration of the analytes measured and the subsequent interpretation of the data...
August 2012: Biomarkers in Medicine
https://www.readbyqxmd.com/read/22916991/the-mbt-adherence-and-competence-scale-mbt-acs-development-structure-and-reliability
#7
Sigmund Karterud, Geir Pedersen, Magnus Engen, Merete Selsbakk Johansen, Paul Niklas Johansson, Christian Schlüter, Oyvind Urnes, Theresa Wilberg, Anthony W Bateman
The properties of the 17-item Mentalization-Based Treatment Adherence and Competence Scale (MBT-ACS) were investigated in a reliability study in which 18 psychotherapy sessions, comprising two sessions by nine different therapists, were rated by seven different raters. The overall reliabilities for adherence and competence for seven raters were high, .84 and .88 respectively. The level of reliability declined by number of raters but was still acceptable for two raters (.60 and .68). The reliabilities for the various items differed...
2013: Psychotherapy Research: Journal of the Society for Psychotherapy Research
https://www.readbyqxmd.com/read/22406166/measurement-of-human-latent-transforming-growth-factor-%C3%AE-1-using-a-latency-associated-protein-reactive-elisa
#8
Irene Areström, Bartek Zuber, Theresa Bengtsson, Niklas Ahlborg
Human Transforming Growth Factor (TGF)-β1, one of three TGF-β isoforms, is a pleotropic cytokine critical for many physiological and immunological processes. TGF-β1 is secreted in a latent form, linked to Latency Associated Protein (LAP). Analysis of Latent TGF-β1 by TGF-β1 ELISA requires dissociation of TGF-β1 from LAP, e.g. by acidification of samples. The ELISA then measures total TGF-β1, equivalent to dissociated Latent TGF-β1 plus any free TGF-β1 present prior to acidification. Evolutionary conservation of TGF-β1 across mammals also renders TGF-β1 ELISAs reactive with TGF-β1 in bovine serum often used in human cell cultures...
May 31, 2012: Journal of Immunological Methods
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