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Daniel M Grochowski, Krystyna Skalicka-Woźniak, Ilkay Erdogan Orhan, Jianbo Xiao, Marcello Locatelli, Jakub P Piwowarski, Sebastian Granica, Michał Tomczyk
Plant tannins are a unique class of polyphenols with relatively high molecular weights. Within the ellagitannins group, agrimoniin--dimeric ellagitannin--is one of the most representative compounds found in many plant materials belonging to the Rosaceae family. Agrimoniin was first isolated in 1982 from roots of Agrimonia pilosa Ledeb. (Rosaceae), a plant traditionally used in Japan and China as an antidiarrheal, hemostatic, and antiparasitic agent. Agrimoniin is a constituent of medicinal plants, which are often applied orally in the form of infusions, decoctions, or tinctures...
July 21, 2017: Annals of the New York Academy of Sciences
Petra Matić, Marija Sabljić, Lidija Jakobek
The aim of this study was to validate spectrophotometric methods for the measurement of total polyphenol (TP;via the Folin–Ciocalteu method) and total flavonoid (TF) content [via the aluminum chloride (AlCl₃) method]. Validation parameters of these methods were determined, including linearity, sensitivity, precision (intra-assay and intermediate), accuracy, LOD, and LOQ. For the validation process, groups of polyphenol standards were used, including phenolic acids (gallic, p-coumaric, caffeic, and chlorogenic acids), flavan-3-ols [(+)-catechin and procyanidins B1 and B2], flavonols (quercetin and quercetin-3-rutinoside), and dihydrochalcones (phloretin and phloretin-2-glucoside)...
July 20, 2017: Journal of AOAC International
Na Li, Zhi-Jun Zhang, Xiao-Jun Li, Hui-Zhen Li, Li-Xia Cui, Dong-Liang He
BACKGROUND: Perilla EO possess high antioxidant, antimicrobial and insecticidal activities, and have proven to be more reliable than chemically synthesized food preservatives. Nevertheless, EOs have disadvantages of facile photo-degradation and oxidation which limits their use in agriculture and food industries. Microencapsulation technology that generates a polymeric coating surrounding EOs could overcome these disadvantages. RESUILS: The EO concentration had a significant effect on encapsulation efficiency (EE) and loading capacity (LC)...
July 18, 2017: Journal of the Science of Food and Agriculture
Eun Woo Kim, Benjamin U Phillips, Christopher J Heath, So Yeon Cho, Hyunjeong Kim, Jemeen Sreedharan, Ho-Taek Song, Jong Eun Lee, Timothy J Bussey, Chul Hoon Kim, Eosu Kim, Lisa M Saksida
Reliable and reproducible assessment of animal learning and behavior is a central aim of basic and translational neuroscience research. Recent developments in automated operant chamber technology have led to the possibility of universal standard protocols, in addition to increased translational potential, reliability and accuracy. However, the impact of regional and national differences in the supplies of available reinforcers in this system on behavioural performance and inter-laboratory variability is an unknown and at present uncontrolled variable...
July 17, 2017: Molecular Brain
Gideon St Helen, Delia A Dempsey, Christopher M Havel, Peyton Jacob, Neal L Benowitz
OBJECTIVES: To describe the effect of e-liquid flavors on nicotine intake and pharmacology of e-cigarettes. METHODS: 11 males and 3 females participated in a 3-day inpatient crossover study with strawberry, tobacco, and their usual flavor e-liquid. Nicotine levels were nominally 18mg/mL in the strawberry (pH 8.29) and tobacco (pH 9.10) e-liquids and ranged between 3-18mg/mL in the usual brands (mean pH 6.80). Each day consisted of a 15-puff session followed by 4h of abstinence, then 90min of ad libitum use...
June 30, 2017: Drug and Alcohol Dependence
Claudia Pinna, Luisa Carta, Vitale Deiana, Ignazio Camarda
The study shows the results of an inventory of place names connected to Arbutus unedo L., a Mediterranean species, widespread throughout Sardinia. The main aim was to compare the past distribution of place names, referring to the strawberry tree, to the current distribution of the species on the island. In addition, we investigated the meaning and the diversity of these local place names in the various communities. The result was a collection of 432 phyto-toponyms. 248 of them were used for an analysis of their distribution in the habitats, indicated on the Map of the Nature System in Sardinia, defined on the basis of the current vegetation typology...
2017: PloS One
Francesca Giampieri, Josè M Alvarez-Suarez, Massimiliano Gasparrini, Tamara Y Forbes-Hernandez, Sadia Afrin, Corrado Rubini, Antonio Zizzi, Josè L Quiles, Bruno Mezzetti, Maurizio Battino
Here, we present new original data on the effects of strawberry consumption on body weight and liver status of aged rats. Wistar rats aged 19-21 months were fed a strawberry enriched diet prepared by substituting 15% of the total calories with freeze-dried strawberry powder for two months. Body weight, plasma biomarkers of liver injury (alanine transferase, aspartate aminotransferase and alkaline phosphatase) and liver histological analysis were assessed. These data indicate that strawberry supplementation did not interfere with normal animal maintenance and with liver structure and functionality...
August 2017: Data in Brief
Meiru Jia, Ning Ding, Qing Zhang, Sinian Xing, Lingzhi Wei, Yaoyao Zhao, Ping Du, Wenwen Mao, Jizheng Li, Bingbing Li, Wensuo Jia
Ripening of fleshy fruits is controlled by a series of intricate signaling processes. Here, we report a FERONIA/FER-like receptor kinase, FaMRLK47, that regulates both strawberry (Fragaria × ananassa) fruit ripening and quality formation. Overexpression and RNAi-mediated downregulation of FaMRLK47 delayed and accelerated fruit ripening, respectively. We showed that FaMRLK47 physically interacts with FaABI1, a negative regulator of abscisic acid (ABA) signaling, and demonstrated that FaMRLK47 regulates fruit ripening by modulating ABA signaling, a major pathway governing strawberry fruit ripening...
2017: Frontiers in Plant Science
Nancy Greco, Nicolás Cluigt, Andrew Cline, Gerardo Liljesthröm
Lobiopa insularis is a newly reported pest of strawberry in Argentina. We investigated characteristics of its biology in the laboratory, including survivorship and reproduction. We also estimated population growth for L. insularis fed ripe strawberry fruits. Lobiopa insularis was not observed ovipositing on strawberry fruits. A higher proportion of egg masses were recorded from a depth of 1 cm within the soil than on either the soil surface or deeper than 1cm (i.e. between 1and 2 cm) within the soil. The duration of preimaginal developmental stages represented ~18...
2017: PloS One
Grace Thompson, Nicola Benwell, Peter Hollingsworth, Andrew McLean-Tooke
Hyperplastic gingivitis is a rare manifestation of granulomatosis with polyangiitis (GPA). This gingivitis has a very distinctive clinical appearance (so-called Strawberry gingivitis) and when seen is virtually pathognomic for GPA. Gingivitis often precedes other organ involvement therefore making awareness of this manifestation particularly important to aid early diagnosis and treatment. Furthermore, histopathological findings of gingival specimens rarely reveal necrotizing granulomatous vasculitis, which is classically seen at other sites of involvement...
June 13, 2017: Seminars in Arthritis and Rheumatism
Javier Tabima, Brent Kronmiller, Caroline Press, Brett M Tyler, Inga Zasada, Niklaus J Grünwald
Phytophthora rubi and P. fragariae are two closely related oomycete plant pathogens that exhibit strong morphological and physiological similarities, but are specialized to infect different hosts of economic importance, namely raspberry and strawberry. Here, we report the draft genome sequences of these two Phytophthora species as a first step towards understanding the genomic processes underlying plant host adaptation in these pathogens.
July 6, 2017: Molecular Plant-microbe Interactions: MPMI
Charles Hawkins, Julie Caruana, Jiaming Li, Chris Zawora, Omar Darwish, Jun Wu, Nadim Alkharouf, Zhongchi Liu
Wild strawberry Fragaria vesca is emerging as an important model system for the cultivated strawberry due to its diploid genome and availability of extensive transcriptome data and a range of molecular genetic tools. Being able to better utilize these tools, especially the transcriptome data, will greatly facilitate research progress in strawberry and other Rosaceae fruit crops. The electronic fluorescent pictograph (eFP) software is a useful and popular tool to display transcriptome data visually, and is widely used in other model organisms including Arabidopsis and mouse...
2017: Horticulture Research
Gabriela M Saavedra, Eugenio Sanfuentes, Pablo M Figueroa, Carlos R Figueroa
The Chilean strawberry (Fragaria chiloensis) fruit has interesting organoleptic properties, but its postharvest life is affected by gray mold decay caused by Botrytis cinerea. The effect of preharvest applications of methyl jasmonate (MeJA) or chitosan on the molecular defense-related responses and protection against gray mold decay were investigated in Chilean strawberry fruit during postharvest storage. Specifically, we inoculated harvested fruit with B. cinerea spores and studied the expression of genes encoding for the pathogenesis-related (PR) proteins β-1,3-glucanases (FcBG2-1, FcBG2-2 and FcBG2-3) and chitinases (FcCHI2-2 and FcCHI3-1), and for polygalacturonase inhibiting proteins (FcPGIP1 and FcPGIP2) at 0, 2, 24, 48, and 72 h post inoculation (hpi)...
July 3, 2017: International Journal of Molecular Sciences
Zijin Zhou, Sophie Zuber, Frédérique Cantergiani, Sophie Butot, Dan Li, Thomas Stroheker, Frank Devlieghere, Anthony Lima, Umberto Piantini, Mieke Uyttendaele
The efficacy of levulinic acid (LVA) in combination with sodium dodecyl sulfate (SDS) in removal of foodborne viruses, enteric bacterial pathogens and their surrogates on fresh strawberries was investigated. Inoculated strawberries were treated with potable water, sodium hypochlorite solution (50ppm), 0.5% LVA plus 0.5% SDS solution, and 5% LVA plus 2% SDS solution respectively for 2min, followed by spray-rinsing with potable water. Water washing removed at least 1.0-log of the tested viral and bacterial strains from the strawberries' surfaces...
June 23, 2017: International Journal of Food Microbiology
Yang Cheng, Jiyun Nie, Zhixia Li, Zhen Yan, Guofeng Xu, Haifei Li, Dikai Guan
In this study, we have successfully synthesized a forchlorfenuron (CPPU)-imprinted polymer in dimethyl sulfoxide by molecular imprinting technique. In the molecular imprinting, β-cyclodextrin was used as the monomer with 1,6-hexamethylene diisocyanate (HMDI) as the cross-linking agent that formed a complex with forchlorfenuron by inclusion interactions. The adsorption equilibrium was attained in approximately 30 min and the maximum binding capacity was 26.79 mg/g, which indicated that the adsorption kinetics was relatively fast...
June 29, 2017: Analytical and Bioanalytical Chemistry
Ane S Simionato, Miguel O P Navarro, Maria L A de Jesus, André R Barazetti, Caroline S da Silva, Glenda C Simões, Maria I Balbi-Peña, João C P de Mello, Luciano A Panagio, Ricardo S C de Almeida, Galdino Andrade, Admilton G de Oliveira
One of the most important postharvest plant pathogens that affect strawberries, grapes and tomatoes is Botrytis cinerea, known as gray mold. The fungus remains in latent form until spore germination conditions are good, making infection control difficult, causing great losses in the whole production chain. This study aimed to purify and identify phenazine-1-carboxylic acid (PCA) produced by the Pseudomonas aeruginosa LV strain and to determine its antifungal activity against B. cinerea. The compounds produced were extracted with dichloromethane and passed through a chromatographic process...
2017: Frontiers in Microbiology
Katrin Franz-Oberdorf, Andreas Langer, Ralf Strasser, Erika Isono, Quirin L Ranftl, Christian Wunschel, Wilfried Schwab
The strawberry fruit allergens Fra a 1.01E, Fra a 1.02 and Fra a 1.03 belong to the group of pathogenesis-related 10 (PR-10) proteins and are homologues of the major birch pollen Bet v 1 and apple allergen Mal d 1. Bet v 1 related proteins are the most extensively studied allergens but their physiological function in planta remains elusive. Since Mal d 1-Associated Protein (MdAP) has been previously identified as interaction partner of Mal d 1 we studied the binding of the orthologous Fra a 1-Associated Protein (FaAP) to Fra a 1...
June 28, 2017: Proteins
Feng Dong, Xiaolin Wang
The present study was aimed to determine the effects of carboxymethyl cellulose (CMC)/garlic essential oil (GEO) composite coatings in improving the quality of strawberries stored at 20°C and 35-40% RH. To find the effects of CMC/GEO composite coatings, strawberries were coated with CMC, CMC+GEO (1%), CMC+GEO (2%), CMC+GEO (3%) and stored, while the uncoated strawberries were taken as control during storing. The effectiveness of CMC/GEO composite coatings was evaluated by measuring their weight loss, decay percentage, ascorbic acid, total phenols, anthocyanins, titratable acidity, total soluble solids and sensory evaluation...
June 26, 2017: International Journal of Biological Macromolecules
Moon-Jin Cho, Soo-Young Park
Carbon dots (CDs) are covalently coupled to the poly(acrylic acid) (PAA) block of polystyrene-b-poly(acrylic acid) (PS-b-PAA). The produced amphiphilic PS-b-PAA-CD shows excellent solubility and micellar formation in selective solvents. Transmission electron microscopy study shows that the micelle of PS-b-PAA-CD in water (a PAA-selective solvent) has a strawberry-like shape with the CDs as patches on the PAA corona surface although that in toluene (a PS-selective solvent) has a reversed shape with the CDs in the core...
July 19, 2017: ACS Applied Materials & Interfaces
Ondřej Kurka, Lukáš Kučera, Helena Pelantová, Marek Kuzma, Vladimír Havlíček, Petr Bednář
Condensation of anthocyanins and their aglycons with small organic molecules yields more stable natural dyes, e.g. pyranoanthocyanins arising spontaneously in various food products. Reaction of pelargonidin with acetone produces two isomeric anthocyanidin dyes - 5-methylpyranopelargonidin and 4-methylfuropelargonidin. A robust semipreparative liquid chromatographic method was developed to isolate both derivatives from a simple aged solution of pelargonidin in methanol: acetone: 37% aqueous hydrochloric acid (1:1:0...
August 11, 2017: Journal of Chromatography. A
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