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https://www.readbyqxmd.com/read/28212568/an-anthocyanin-rich-strawberry-extract-induces-apoptosis-and-ros-while-decreases-glycolysis-and-fibrosis-in-human-uterine-leiomyoma-cells
#1
Md Soriful Islam, Francesca Giampieri, Milijana Janjusevic, Massimiliano Gasparrini, Tamara Y Forbes-Hernandez, Luca Mazzoni, Stefania Greco, Stefano Raffaele Giannubilo, Andrea Ciavattini, Bruno Mezzetti, Franco Capocasa, Mario Castellucci, Maurizio Battino, Pasquapina Ciarmela
Uterine leiomyomas are highly prevalent benign tumors in reproductive aged women. Unfortunately, medical treatments are still limited and no preventive therapies have been developed. In the present study, we investigated the therapeutic effects of strawberry extract on uterine leiomyoma cells. Leiomyoma and myometrial cells were treated with strawberry (cultivar Alba) extract (250 μg/ml) for 48 h to measure apoptosis, reactive oxygen species (ROS), oxidative phosphorylation (OCR, oxygen consumption rate) and glycolysis (ECAR, extracellular acidification rate) as well as fibrosis associated gene and/or protein expression...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28205186/optimisation-of-cognitive-performance-in-rodent-operant-touchscreen-testing-evaluation-and-effects-of-reinforcer-strength
#2
Benjamin U Phillips, Christopher J Heath, Zofia Ossowska, Timothy J Bussey, Lisa M Saksida
Operant testing is a widely used and highly effective method of studying cognition in rodents. Performance on such tasks is sensitive to reinforcer strength. It is therefore advantageous to select effective reinforcers to minimize training times and maximize experimental throughput. To quantitatively investigate the control of behavior by different reinforcers, performance of mice was tested with either strawberry milkshake or a known powerful reinforcer, super saccharin (1.5% or 2% (w/v) saccharin/1.5% (w/v) glucose/water mixture)...
February 15, 2017: Learning & Behavior
https://www.readbyqxmd.com/read/28195221/transcriptome-analysis-around-the-onset-of-strawberry-fruit-ripening-uncovers-an-important-role-of-oxidative-phosphorylation-in-ripening
#3
Qing-Hua Wang, Cheng Zhao, Miao Zhang, Yu-Zhong Li, Yuan-Yue Shen, Jia-Xuan Guo
Although much progress has been made towards understanding the ripening of non-climacteric fruit using the strawberry as a model plant, the defined molecular mechanisms remain unclear. Here, RNA-sequencing was performed using four cDNA libraries around the onset of ripening, and a total of 31,793 unigenes and 335 pathways were annotated including the top five pathways, which were involved in ribosome, spliceosome, protein processing, plant-pathogen interaction and plant hormone signaling, and the important DEGs related to ripening were annotated to be mainly involved in protein translation and processing, sugar metabolism, energy metabolism, phytohormones, antioxidation, pigment and softening, especially finding a decreased trend of oxidative phosphorylation during red-coloring...
February 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28194699/efficacy-and-safety-of-2-topical-propranolol-cream-for-the-treatment-of-proliferating-infantile-strawberry-hemangiomas
#4
Yuanyuan Wang, Xingcun Zhang, Yadong Yang, Junbo Zhang, Yunchuan Yang, Yuangang Lu
OBJECTIVE: To evaluate the efficacy and safety of 2% topical propranolol cream in the treatment of proliferating infantile strawberry hemangiomas. METHODS: A total of 40 infants were enrolled; 2% propranolol cream was applied three times daily. In the subsequent monthly visit, dynamic changes in tumor size, texture, and color were recorded. The adverse events (AEs) were observed. Treatment outcomes were scored on a four-point scale. All patients were followed up for 12 mo after treatment...
February 14, 2017: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/28168102/endophytic-fungal-diversity-of-fragaria-vesca-a-crop-wild-relative-of-strawberry-along-environmental-gradients-within-a-small-geographical-area
#5
Kazutomo Yokoya, Sarah Postel, Rui Fang, Viswambharan Sarasan
BACKGROUND: Fungal endophytes are highly diverse ubiquitous asymptomatic microorganisms, some of which appear to be symbiotic. Depending on abiotic conditions and genotype of the plant, the diversity of endophytes may confer fitness benefits to plant communities. METHODS: We studied a crop wild relative (CWR) of strawberry, along environmental gradients with a view to understand the cultivable root-derived endophytic fungi that can be evaluated for promoting growth and tolerating stress in selected plant groups...
2017: PeerJ
https://www.readbyqxmd.com/read/28167891/construction-of-a-system-for-the-strawberry-nursery-production-towards-elimination-of-latent-infection-of-anthracnose-fungi-by-a-combination-of-pcr-and-microtube-hybridization
#6
Kazuyoshi Furuta, Saki Nagashima, Tsuyoshi Inukai, Chikara Masuta
One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae...
February 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28159266/unravelling-the-nanostructure-of-strawberry-fruit-pectins-by-endo-polygalacturonase-digestion-and-atomic-force-microscopy
#7
Candelas Paniagua, Andrew R Kirby, A Patrick Gunning, Victor J Morris, Antonio J Matas, Miguel A Quesada, José A Mercado
Pectins analysed by AFM are visualized as individual chains, branched or unbranched, and aggregates. To investigate the nature of these structures, sodium carbonate soluble pectins from strawberry fruits were digested with endo-polygalacturonase M2 from Aspergillus aculeatus and visualized by AFM. A gradual decrease in the length of chains was observed as result of the treatment, reaching a minimum LN value of 22nm. The branches were not visible after 2h of enzymatic incubation. The size of complexes also diminished significantly with the enzymatic digestion...
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28154491/first-report-of-dieback-caused-by-lasiodiplodia-theobromae-in-strawberry-plants-in-korea
#8
Myeong Hyeon Nam, Myung Soo Park, Hyun Sook Kim, Tae Il Kim, Eun Mo Lee, Jong Dae Park, Hong Gi Kim
Dieback in strawberry (Seolhyang cultivar) was first observed during the nursery season (June to September) in the Nonsan area of Korea in the years 2012 and 2013. Initial disease symptoms included dieback on runners, as well as black rot on roots, followed by wilting and eventually blackened, necrotic discoloration in the crowns of daughter plants. A fungus isolated from the diseased roots, runners, and crowns is close to Lasiodiplodia theobromae based on morphological characteristics. Analysis of a combined dataset assembled from sequences of the internal transcribed spacer and translation elongation factor 1-alpha genes grouped nine fungal isolates with the type strain of L...
December 2016: Mycobiology
https://www.readbyqxmd.com/read/28142191/partial-preferential-chromosome-pairing-is-genotype-dependent-in-tetraploid-rose
#9
Peter M Bourke, Paul Arens, Roeland E Voorrips, G Danny Esselink, Carole F S Koning-Boucoiran, Wendy P C van 't Westende, Tiago Santos Leonardo, Patrick Wissink, Chaozhi Zheng, Geert van Geest, Richard G F Visser, Frans A Krens, Marinus J M Smulders, Chris Maliepaard
It has long been recognised that polyploid species do not always neatly fall into the categories of auto- or allopolyploid, leading to the term "segmental allopolyploid" to describe everything in between. The meiotic behaviour of such intermediate species is not fully understood, nor is there consensus as to how to model their inheritance patterns. In this study, we used a tetraploid cut rose (Rosa hybrida) population, genotyped using the 68K WagRhSNP array, to construct an ultra-high density linkage map of all homologous chromosomes, using methods previously developed for autotetraploids...
January 31, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28139162/effect-of-pasteurisation-and-freezing-method-on-bioactive-compounds-and-antioxidant-activity-of-strawberry-pulp
#10
Gilma Auxiliadora Santos Gonçalves, Nathane Silva Resende, Elisângela Elena Nunes Carvalho, Jaime Vilela de Resende, Eduardo Valério de Barros Vilas Boas
This study evaluated the stability of strawberry pulp subjected to three factors, pasteurisation (pasteurised and unpasteurised), freezing method (static air and forced air) and storage time (0, 2, 4 and 6 months). Pasteurisation favoured vitamin C retention during storage but enhanced the total loss of phenolics without affecting anthocyanin levels. Freezing by forced air was more effective in retaining phenolics during the first 4 months of storage, although the freezing method did not affect the anthocyanin levels...
January 31, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28135760/landscape-scale-gpp-and-carbon-density-inform-patterns-and-impacts-of-an-invasive-tree-across-wet-forests-of-hawaii
#11
Jomar M Barbosa, Gregory P Asner, R Flint Hughes, M Tracy Johnson
Plant invasion typically occurs within a landscape-scale framework of abiotic and biotic conditions, often resulting in emergent feedbacks among environment, ecosystem functions, and the dominance of invasive species. Understanding the mechanisms underlying successful invasions is an important component of conservation and management efforts, but this has been poorly investigated in a spatially explicit manner. Knowing where and why invasion patterns change throughout the landscape enables managers to use context-specific controls on the spread of invasive species...
October 3, 2016: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/28130090/anti-inflammatory-effect-of-strawberry-extract-against-lps-induced-stress-in-raw-264-7-macrophages
#12
Massimiliano Gasparrini, Tamara Y Forbes-Hernandez, Francesca Giampieri, Sadia Afrin, Josè M Alvarez-Suarez, Luca Mazzoni, Bruno Mezzetti, Josè L Quiles, Maurizio Battino
A common denominator in the pathogenesis of most chronic inflammatory diseases is the involvement of oxidative stress, related to ROS production by all aerobic organisms. Dietary antioxidants from plant foods represent an efficient strategy to counteract this condition. The aim of the present study was to evaluate the protective effects of strawberry extracts on inflammatory status induced by E. Coli LPS on RAW 264.7 macrophages by measuring the main oxidative and inflammatory biomarkers and investigating the molecular pathways involved...
January 25, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28117711/protective-effect-of-strawberry-extract-against-inflammatory-stress-induced-in-human-dermal-fibroblasts
#13
Massimiliano Gasparrini, Tamara Y Forbes-Hernandez, Francesca Giampieri, Sadia Afrin, Bruno Mezzetti, Josè L Quiles, Stefano Bompadre, Maurizio Battino
A protracted pro-inflammatory state is a major contributing factor in the development, progression and complication of the most common chronic pathologies. Fruit and vegetables represent the main sources of dietary antioxidants and their consumption can be considered an efficient tool to counteract inflammatory states. In this context an evaluation of the protective effects of strawberry extracts on inflammatory stress induced by E. coli LPS on human dermal fibroblast cells was performed in terms of viability assays, ROS and nitrite production and biomarkers of oxidative damage of the main biological macromolecules...
January 21, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28116068/cryptic-female-strawberry-poison-frogs-experience-elevated-predation-risk-when-associating-with-an-aposematic-partner
#14
Julia Carolina Segami Marzal, Andreas Rudh, Björn Rogell, Anders Ödeen, Hanne Løvlie, Charlotte Rosher, Anna Qvarnström
Population divergence in sexual signals may lead to speciation through prezygotic isolation. Sexual signals can change solely due to variation in the level of natural selection acting against conspicuousness. However, directional mate choice (i.e., favoring conspicuousness) across different environments may lead to gene flow between populations, thereby delaying or even preventing the evolution of reproductive barriers and speciation. In this study, we test whether natural selection through predation upon mate-choosing females can favor corresponding changes in mate preferences...
January 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28115904/evaluation-of-polyphenol-content-and-antioxidant-capacity-of-fruits-and-vegetables-using-a-modified-enzymatic-extraction
#15
Rudy Álvarez, Héctor Araya, Rosa Navarro-Lisboa, Carol Lopez de Dicastillo
Fruits and vegetables are considered a good source of polyphenols and antioxidant capacities which are beneficial in protecting the human body against damage induced by reactive species. The objective of this work is to conduct an assessment of the polyphenol content and antioxidant activities of different fruit (kiwi, pear, green apple, raspberry, blackberry, strawberry and blueberry) and vegetable (pumpkin, green and red pepper) extracts using both chemical extraction and a modified in vitro digestive enzymatic extraction in order to compare results...
December 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/28110594/protective-effect-of-fragaria-ananassa-methanolic-extract-on-cadmium-chloride-cdcl2-induced-hepatotoxicity-in-rats
#16
Manal F Elkhadragy, Ahmed E Abdel Moneim
This study investigated the protective effect of Fragaria ananassa methanolic extract on cadmium chloride (CdCl2)-induced hepatotoxicity in rats. CdCl2 was intraperitoneally injected at a dose of 6.5 mg/kg of body weight for 5 days with or without methanol extract of Fragaria ananassa (250 mg/kg). The hepatic cadmium concentration, lipid peroxidation, nitric oxide, glutathione content and antioxidant enzyme activities, including superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, were estimated...
January 22, 2017: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/28107986/dispersive-solid-phase-extraction-using-polyaniline-modified-zeolite-nay-as-a-new-sorbent-for-multiresidue-analysis-of-pesticides-in-food-and-environmental-samples
#17
Prapha Arnnok, Nopbhasinthu Patdhanagul, Rodjana Burakham
The applicability of polyaniline (PANI)-modified zeolite NaY as a sorbent for multi-class pesticides extraction was investigated. The sorbent had a sorption capacity of 833mgkg(-1) sorbent and preconcentration factor of 42 in the mixture of twenty pesticides. The PANI-coated zeolite NaY was successfully created via oxidative polymerization of aniline onto the surface of the zeolite. The sorbent was applied for dispersive solid phase extraction (DSPE) of commonly used pesticides belonging to five different chemical groups, involving carbamate, organophosphate, sulfonylurea, pyrethroid and neonicotinoid...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28101741/anti-hyperuricemic-effect-of-taxifolin-in-cultured-hepatocytes-and-model-mice
#18
Shin-Ichi Adachi, Ken-Ichi Nihei, Yoshiyuki Ishihara, Fumiaki Yoshizawa, Kazumi Yagasaki
Hyperuricemia is recognized as an important risk factor for gout. High dietary intake of purine-rich foods such as meats and sea foods increases uric acid (UA) levels in the blood. Taxifolin present in Siberian larch and strawberries has been reported to possess health promoting activities including anti-oxidant effect. In this study, we examined anti-hyperuricemic effect of taxifolin in both cultured hepatocytes and hyperuricemic model mice. In cultured AML12 hepatocytes, taxifolin significantly suppressed UA production dose- and time-dependently...
January 18, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28099767/a-comparative-study-of-phenolic-antioxidant-activity-and-flavonoid-biosynthesis-related-gene-expression-between-summer-and-winter-strawberry-cultivars
#19
Doori Park, Yeri Park, Young Hun Lee, Ik-Young Choi, Kyong Cheul Park, Sang Un Park, Byung Sup Kim, Young Rog Yeoung, Nam Il Park
Strawberry (Fragaria ananassa Duch.) possesses good antioxidant properties. Phenolic compounds in strawberries, such as anthocyanins and ellagic acid, mainly act as antioxidants. This study aimed to compare the phenolic content and expression patterns of genes involved in flavonoid biosynthesis between summer and winter strawberry cultivars affected by seasonal variation, degree of ripeness, and genotype. Antioxidant activity and the total content of phenols and flavonoids decreased with fruit ripening. Most notably, summer strawberry cultivars showed higher antioxidant activity than winter cultivars...
January 18, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28090334/an-experimental-validation-of-genomic-selection-in-octoploid-strawberry
#20
Salvador A Gezan, Luis F Osorio, Sujeet Verma, Vance M Whitaker
The primary goal of genomic selection is to increase genetic gains for complex traits by predicting performance of individuals for which phenotypic data are not available. The objective of this study was to experimentally evaluate the potential of genomic selection in strawberry breeding and to define a strategy for its implementation. Four clonally replicated field trials, two in each of 2 years comprised of a total of 1628 individuals, were established in 2013-2014 and 2014-2015. Five complex yield and fruit quality traits with moderate to low heritability were assessed in each trial...
2017: Horticulture Research
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