keyword
MENU ▼
Read by QxMD icon Read
search

zhaojing

keyword
https://www.readbyqxmd.com/read/29103021/hsa_circ_0000520-a-potential-new-circular-rna-biomarker-is-involved-in-gastric-carcinoma
#1
Handong Sun, Weiwei Tang, Dawei Rong, Hui Jin, Kai Fu, Wenling Zhang, Zhaojing Liu, Hongyong Cao, Xiufeng Cao
BACKGROUND: Circular RNAs (circRNAs) have been found playing important roles in regulating cancer progression. Human circRNA microarray was performed to screen for abnormally expressed circRNA in gastric cancer tissues. In this study, we are aimed to investigate the relationship between a new circular RNA named hsa_circ_0000520 and gastric cancer development. METHODS: The hsa_circ_0000520 levels were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in gastric tissue, cell and plasma levels, respectively...
October 18, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28981088/hsf4-regulates-lens-fiber-cell-differentiation-by-activating-p53-and-its-downstream-regulators
#2
Meng Gao, Yuwen Huang, Ling Wang, Mi Huang, Fei Liu, Shengjie Liao, Shanshan Yu, Zhaojing Lu, Shanshan Han, Xuebin Hu, Zhen Qu, Xiliang Liu, Tinsae Assefa Yimer, Lifang Yang, Zhaohui Tang, David Wan-Cheng Li, Mugen Liu
Cataract refers to opacities of the lens that impede the passage of light. Mutations in heat shock transcription factor 4 (HSF4) have been associated with cataract; however, the mechanisms regarding how mutations in HSF4 cause cataract are still obscure. In this study, we generated an hsf4 knockout zebrafish model using TALEN technology. The mutant zebrafish developed an early-onset cataract with multiple developmental defects in lens. The epithelial cells of the lens were overproliferated, resulting in the overabundance of lens fiber cells in hsf4(null) zebrafish lens...
October 5, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28959248/rifabutin-resistance-associated-with-double-mutations-in-rpob-gene-in-mycobacterium-tuberculosis-isolates
#3
Wei Jing, Yu Pang, Zhaojing Zong, Jing Wang, Ru Guo, Fengmin Huo, Guanglu Jiang, Yifeng Ma, Hairong Huang, Naihui Chu
The objective of this study was to investigate the cross-resistance between rifampin (RIF) and rifabutin (RFB) among clinical Mycobacterium tuberculosis (MTB) isolates, and the correlations between specific rpoB mutations and the minimum inhibitory concentrations (MICs) of RIF and RFB. A total of 256 RIF-resistant isolates were included from the National Tuberculosis Clinical Laboratory in China. The MICs of MTB isolates against RIF and RFB were determined by using a microplate alamarBlue assay. In addition, all the MTB isolates were sequenced for mutations in rpoB gene...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28926148/biodiversity-and-species-competition-regulate-the-resilience-of-microbial-biofilm-community
#4
Kai Feng, Zhaojing Zhang, Weiwei Cai, Wenzong Liu, Meiying Xu, Huaqun Yin, Aijie Wang, Zhili He, Ye Deng
The relationship between biodiversity and ecosystem stability is poorly understood in microbial communities. Biofilm communities in small bioreactors called microbial electrolysis cells (MEC) contain moderate species numbers and easy tractable functional traits, thus providing an ideal platform for verifying ecological theories in microbial ecosystems. Here, we investigated the resilience of biofilm communities with a gradient of diversity, and explored the relationship between biodiversity and stability in response to a pH shock...
September 19, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28752308/green-synthesis-of-gold-nanoparticles-using-fungus-mariannaea-sp-hj-and-their-catalysis-in-reduction-of-4-nitrophenol
#5
Xiaofang Pei, Yuanyuan Qu, Wenli Shen, Huijie Li, Xuwang Zhang, Shuzhen Li, Zhaojing Zhang, Xuanying Li
In the present study, biosynthesis of gold nanoparticles (AuNPs) by the cells (cells-AuNPs) and cell-free extracts (extracts-AuNPs) of a new fungus Mariannaea sp. HJ was reported. The as-synthesized particles were characterized by UV-vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The effects of different parameters on AuNP biosynthesis were investigated, and initial gold ion concentration of 2 mM, pH 7, was demonstrated to be suitable for both cells-AuNP and extracts-AuNP syntheses...
September 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28739779/in-vitro-drug-susceptibility-of-bedaquiline-delamanid-linezolid-clofazimine-moxifloxacin-and-gatifloxacin-against-extensively-drug-resistant-tuberculosis-in-beijing-china
#6
Yu Pang, Zhaojing Zong, Fengmin Huo, Wei Jing, Yifeng Ma, Lingling Dong, Yunxu Li, Liping Zhao, Yuhong Fu, Hairong Huang
Extensively drug-resistant tuberculosis (XDR-TB) is a deadly form of TB that can be incurable due to its extreme drug resistance. In this study, we aimed to explore the in vitro susceptibility to bedaquiline (BDQ), delamanid (DMD), linezolid (LZD), clofazimine (CLO), moxifloxacin (MFX), and gatifloxacin (GAT) of 90 XDR-TB strains isolated from patients in China. We also describe the genetic characteristics of XDR-TB isolates with acquired drug resistance. Resistance to MFX, GAT, LZD, CLO, DMD, and BDQ was found in 82 (91...
October 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28684789/soil-bacterial-quantification-approaches-coupling-with-relative-abundances-reflecting-the-changes-of-taxa
#7
Zhaojing Zhang, Yuanyuan Qu, Shuzhen Li, Kai Feng, Shang Wang, Weiwei Cai, Yuting Liang, Hui Li, Meiying Xu, Huaqun Yin, Ye Deng
Understanding the abundance change of certain bacterial taxa is quite important for the study of soil microbiology. However, the observed differences of relative abundances by high-throughput techniques may not accurately reflect those of the actual taxon abundances. This study investigated whether soil microbial abundances coupling with microbial quantities can be more informative in describing the microbial population distribution under different locations. We analyzed relative abundances of the major species in soil microbial communities from Beijing and Tibet grasslands by using 16 S rRNA high-throughput sequencing technique, and quantified the absolute bacterial cell numbers directly or indirectly by multiple culture-independent measurements, including adenosine tri-phosphate (ATP), flow cytometry (FCM), quantitative real-time PCR (qPCR), phospholipid fatty acids (PLFA) and microbial biomass Carbon (MBC)...
July 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28530386/controllable-growth-of-ga-film-electrodeposited-from-aqueous-solution-and-cu-in-ga-se2-solar-cells
#8
Jinlian Bi, Jianping Ao, Qing Gao, Zhaojing Zhang, Guozhong Sun, Qing He, Zhiqiang Zhou, Yun Sun, Yi Zhang
Electrodepositon of Ga film is very challenging due to the high standard reduction potential (-0.53 V vs SHE for Ga(3+)). In this study, Ga film with compact structure was successfully deposited on the Mo/Cu/In substrate by the pulse current electrodeposition (PCE) method using GaCl3 aqueous solution. A high deposition rate of Ga(3+) and H(+) can be achieved by applying a large overpotential induced by high pulse current. In the meanwhile, the concentration polarization induced by cation depletion can be minimized by changing the pulse frequency and duty cycle...
June 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28398482/cerkl-gene-knockout-disturbs-photoreceptor-outer-segment-phagocytosis-and-causes-rod-cone-dystrophy-in-zebrafish
#9
Shanshan Yu, Chang Li, Lincoln Biswas, Xuebin Hu, Fei Liu, James Reilly, Xiliang Liu, Ying Liu, Yuwen Huang, Zhaojing Lu, Shanshan Han, Lei Wang, Jing Yu Liu, Tao Jiang, Xinhua Shu, Fulton Wong, Zhaohui Tang, Mugen Liu
In humans, CERKL mutations cause widespread retinal degeneration: early dysfunction and loss of rod and cone photoreceptors in the outer retina and, progressively, death of cells in the inner retina. Despite intensive efforts, the function of CERKL remains obscure and studies in animal models have failed to clarify the disease mechanism of CERKL mutations. To address this gap in knowledge, we have generated a stable CERKL knockout zebrafish model by TALEN technology and a 7bp deletion in CERKL cDNA that caused the premature termination of CERKL...
June 15, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28378834/ablation-of-eys-in-zebrafish-causes-mislocalisation-of-outer-segment-proteins-f-actin-disruption-and-cone-rod-dystrophy
#10
Zhaojing Lu, Xuebin Hu, Fei Liu, Dinesh C Soares, Xiliang Liu, Shanshan Yu, Meng Gao, Shanshan Han, Yayun Qin, Chang Li, Tao Jiang, Daji Luo, An-Yuan Guo, Zhaohui Tang, Mugen Liu
Mutations in EYS are associated with autosomal recessive retinitis pigmentosa (arRP) and autosomal recessive cone-rod dystrophy (arCRD) however, the function of EYS and the molecular mechanisms of how these mutations cause retinal degeneration are still unclear. Because EYS is absent in mouse and rat, and the structure of the retina differs substantially between humans and Drosophila, we utilised zebrafish as a model organism to study the function of EYS in the retina. We constructed an EYS-knockout zebrafish-line by TALEN technology which showed visual impairment at an early age, while the histological and immunofluorescence assays indicated the presence of progressive retinal degeneration with a cone predominately affected pattern...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28209709/pathogenic-mutations-in-retinitis-pigmentosa-2-predominantly-result-in-loss-of-rp2-protein-stability-in-humans-and-zebrafish
#11
Fei Liu, Yayun Qin, Shanshan Yu, Dinesh C Soares, Lifang Yang, Jun Weng, Chang Li, Meng Gao, Zhaojing Lu, Xuebin Hu, Xiliang Liu, Tao Jiang, Jing Yu Liu, Xinhua Shu, Zhaohui Tang, Mugen Liu
Mutations in retinitis pigmentosa 2 (RP2) account for 10-20% of X-linked retinitis pigmentosa (RP) cases. The encoded RP2 protein is implicated in ciliary trafficking of myristoylated and prenylated proteins in photoreceptor cells. To date >70 mutations in RP2 have been identified. How these mutations disrupt the function of RP2 is not fully understood. Here we report a novel in-frame 12-bp deletion (c.357_368del, p.Pro120_Gly123del) in zebrafish rp2 The mutant zebrafish shows reduced rod phototransduction proteins and progressive retinal degeneration...
April 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28154170/transforming-growth-factor-%C3%AE-tgf-%C3%AE-directly-activates-the-jak1-stat3-axis-to-induce-hepatic-fibrosis-in-coordination-with-the-smad-pathway
#12
Liu-Ya Tang, Mary Heller, Zhaojing Meng, Li-Rong Yu, Yi Tang, Ming Zhou, Ying E Zhang
Transforming growth factor-β (TGF-β) signals through both SMAD and non-SMAD pathways to elicit a wide array of biological effects. Existing data have shown the association and coordination between STATs and SMADs in mediating TGF-β functions in hepatic cells, but it is not clear how STATs are activated under these circumstances. Here, we report that JAK1 is a constitutive TGFβRI binding protein and is absolutely required for phosphorylation of STATs in a SMAD-independent manner within minutes of TGF-β stimulation...
March 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28149995/trophic-transfer-of-hexabromocyclododecane-in-the-terrestrial-and-aquatic-food-webs-from-an-e-waste-dismantling-region-in-east-china
#13
Chaofei Zhu, Pu Wang, Yingming Li, Zhaojing Chen, Honghua Li, Patrick Ssebugere, Qinghua Zhang, Guibin Jiang
Trophic transfer of hexabromocyclododecane (HBCD) was investigated in both the terrestrial and aquatic food webs from an e-waste dismantling region in East China. The mean Σ3HBCD concentrations in the terrestrial species varied from 0.91 (0.16-1.85) ng g(-1) lipid weight (lw) in dragonflies (Pantala flavescens) to 40.3 (22.1-51.1) ng g(-1) lw in rats (Rattus norvegicus). The isomeric profile indicated that α-HBCD presented a decreasing trend along the trophic level (TL) (from 97.2% to 16.3% of Σ3HBCDs), while γ-HBCD showed a reverse trend (from 2...
February 22, 2017: Environmental Science. Processes & Impacts
https://www.readbyqxmd.com/read/28143869/stabilization-of-notch1-by-the-hsp90-chaperone-is-crucial-for-t-cell-leukemogenesis
#14
Zhaojing Wang, Yufeng Hu, Daibiao Xiao, Jingchao Wang, Chuntao Liu, Yisheng Xu, Xiaomeng Shi, Peng Jiang, Liang Huang, Peng Li, Hudan Liu, Guoliang Qing
Purpose: Notch1 deregulation is assuming a focal role in T-cell acute lymphoblastic leukemia (T-ALL). Despite tremendous advances in our understanding of Notch1 transcriptional programs, the mechanisms by which Notch1 stability and turnover are regulated remain obscure. The goal of the current study is to identify intracellular Notch1 (ICN1, the activated form of Notch1) binding partner(s) regulating its stability and activity.Experimental Design: We employed immunoaffinity purification to identify ICN1-associating partner(s) and used coimmunoprecipitation to verify the endogenous protein interaction...
July 15, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27685320/quantitative-structure-activity-relationship-models-for-the-antioxidant-activity-of-polysaccharides
#15
Zhiming Li, Kaiying Nie, Zhaojing Wang, Dianhui Luo
In this study, quantitative structure activity relationship (QSAR) models for the antioxidant activity of polysaccharides were developed with 50% effective concentration (EC50) as the dependent variable. To establish optimum QSAR models, multiple linear regressions (MLR), support vector machines (SVM) and artificial neural networks (ANN) were used, and 11 molecular descriptors were selected. The optimum QSAR model for predicting EC50 of DPPH-scavenging activity consisted of four major descriptors. MLR model gave EC50 = 0...
2016: PloS One
https://www.readbyqxmd.com/read/27637096/catalytic-reduction-of-4-nitrophenol-using-gold-nanoparticles-biosynthesized-by-cell-free-extracts-of-aspergillus-sp-wl-au
#16
Wenli Shen, Yuanyuan Qu, Xiaofang Pei, Shuzhen Li, Shengnan You, Jingwei Wang, Zhaojing Zhang, Jiti Zhou
A facile one-pot eco-friendly process for synthesis of gold nanoparticles (AuNPs) with high catalytic activity was achieved using cell-free extracts of Aspergillus sp. WL-Au as reducing, capping and stabilizing agents. The surface plasmon resonance band of UV-vis spectrum at 532nm confirmed the presence of AuNPs. Transmission electron microscopy images showed that quite uniform spherical AuNPs were synthesized and the average size of nanoparticles increased from 4nm to 29nm with reaction time. X-ray diffraction analysis verified the formation of nano-crystalline gold particles...
January 5, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/27572304/role-of-il-17-pathways-in-immune-privilege-a-rna-deep-sequencing-analysis-of-the-mice-testis-exposure-to-fluoride
#17
Meijun Huo, Haijun Han, Zilong Sun, Zhaojing Lu, Xinglei Yao, Shaolin Wang, Jundong Wang
We sequenced RNA transcripts from the testicles of healthy male mice, divided into a control group with distilled water and two experimental groups with 50 and 100 mg/l NaF in drinking water for 56 days. Bowtie/Tophat were used to align 50-bp paired-end reads into transcripts, Cufflinks to measure the relative abundance of each transcript and IPA to analyze RNA-Sequencing data. In the 100 mg/l NaF-treated group, four pathways related to IL-17, TGF-β and other cellular growth factor pathways were overexpressed...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27516247/structure-elucidation-of-a-non-branched-and-entangled-heteropolysaccharide-from-tremella-sanguinea-peng-and-its-antioxidant-activity
#18
Zhaojing Wang, Yawei Zeng, Dianhui Luo
A crude polysaccharide was extracted from the edible fungi Tremella sanguinea Peng, and a polysaccharide TSP-II (31.56%) was separated and purified from the crude polysaccharide. TSP-II was a homogeneous polysaccharide by the high-performance size-exclusion chromatography (HPSEC), had a molecular weight of 356kD and consisted mainly of mannose, xylose, galactose and glucose at a molar ratio 5.9:2.4:1:1.1. The structural assignment of TSP-II was carried out using fourier transform infrared spectroscopy (FTIR) analysis, periodate oxidation-smith degradation, partial hydrolysis with acid, methylation analysis and nuclear magnetic resonance (NMR) studies, and the repeating unit of TSP-II was thus determined...
November 5, 2016: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27454120/pdcd4-is-involved-in-the-formation-of-stress-granule-in-response-to-oxidized-low-density-lipoprotein-or-high-fat-diet
#19
Yang Bai, Zhaojing Dong, Qianwen Shang, Hui Zhao, Liyang Wang, Chun Guo, Fei Gao, Lining Zhang, Qun Wang
Stress granules (SGs) in response to various stresses have been reported in many diseases. We previously reported the implication of programmed cell death 4 (Pdcd4) in obesity-induced stress responses, but the possible link between Pdcd4 and SGs remains lacking. In this study we showed that oxidized low-density lipoprotein (ox-LDL) or high-fat diet (HFD) induced SG formation in mouse macrophages and liver tissues, and Pdcd4 deficiency in mice remarkably reduced its formation. In response to ox-LDL, either endogenous or ectopic Pdcd4 displayed granule-like expression and co-localized with SG markers including T-cell-restricted intracellular antigen-1, fragile X mental retardation-related protein 1, and eukaryotic initiation factor 4A...
2016: PloS One
https://www.readbyqxmd.com/read/27395407/three-novel-missense-mutations-in-the-filamin-b-gene-are-associated-with-isolated-congenital-talipes-equinovarus
#20
Haiou Yang, Zhaojing Zheng, Haiqing Cai, Huimin Li, Xingchen Ye, Xiaoqing Zhang, Zhigang Wang, Qihua Fu
Congenital talipes equinovarus (CTEV) is one of the most common musculoskeletal disorders. Genetic factors have been suggested to be an important contributor to its pathogenesis. Some genes, including PITX1, TBX4, and RBM10, have been associated with CTEV. We aimed to determine the disease-causing mutations in Chinese patients with isolated CTEV. Genomic DNA was extracted from peripheral blood samples of a three-generation pedigree and 53 sporadic patients with CTEV. Whole-exome sequencing and Sanger sequencing were used to identify and validate disease-causing mutations, respectively...
October 2016: Human Genetics
keyword
keyword
96862
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"