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Zhiming Li, Kaiying Nie, Zhaojing Wang, Dianhui Luo
In this study, quantitative structure activity relationship (QSAR) models for the antioxidant activity of polysaccharides were developed with 50% effective concentration (EC50) as the dependent variable. To establish optimum QSAR models, multiple linear regressions (MLR), support vector machines (SVM) and artificial neural networks (ANN) were used, and 11 molecular descriptors were selected. The optimum QSAR model for predicting EC50 of DPPH-scavenging activity consisted of four major descriptors. MLR model gave EC50 = 0...
2016: PloS One
Wenli Shen, Yuanyuan Qu, Xiaofang Pei, Shuzhen Li, Shengnan You, Jingwei Wang, Zhaojing Zhang, Jiti Zhou
A facile one-pot eco-friendly process for synthesis of gold nanoparticles (AuNPs) with high catalytic activity was achieved using cell-free extracts of Aspergillus sp. WL-Au as reducing, capping and stabilizing agents. The surface plasmon resonance band of UV-vis spectrum at 532nm confirmed the presence of AuNPs. Transmission electron microscopy images showed that quite uniform spherical AuNPs were synthesized and the average size of nanoparticles increased from 4nm to 29nm with reaction time. X-ray diffraction analysis verified the formation of nano-crystalline gold particles...
July 21, 2016: Journal of Hazardous Materials
Meijun Huo, Haijun Han, Zilong Sun, Zhaojing Lu, Xinglei Yao, Shaolin Wang, Jundong Wang
We sequenced RNA transcripts from the testicles of healthy male mice, divided into a control group with distilled water and two experimental groups with 50 and 100 mg/l NaF in drinking water for 56 days. Bowtie/Tophat were used to align 50-bp paired-end reads into transcripts, Cufflinks to measure the relative abundance of each transcript and IPA to analyze RNA-Sequencing data. In the 100 mg/l NaF-treated group, four pathways related to IL-17, TGF-β and other cellular growth factor pathways were overexpressed...
2016: Scientific Reports
Zhaojing Wang, Yawei Zeng, Dianhui Luo
A crude polysaccharide was extracted from the edible fungi Tremella sanguinea Peng, and a polysaccharide TSP-II (31.56%) was separated and purified from the crude polysaccharide. TSP-II was a homogeneous polysaccharide by the high-performance size-exclusion chromatography (HPSEC), had a molecular weight of 356kD and consisted mainly of mannose, xylose, galactose and glucose at a molar ratio 5.9:2.4:1:1.1. The structural assignment of TSP-II was carried out using fourier transform infrared spectroscopy (FTIR) analysis, periodate oxidation-smith degradation, partial hydrolysis with acid, methylation analysis and nuclear magnetic resonance (NMR) studies, and the repeating unit of TSP-II was thus determined...
November 5, 2016: Carbohydrate Polymers
Yang Bai, Zhaojing Dong, Qianwen Shang, Hui Zhao, Liyang Wang, Chun Guo, Fei Gao, Lining Zhang, Qun Wang
Stress granules (SGs) in response to various stresses have been reported in many diseases. We previously reported the implication of programmed cell death 4 (Pdcd4) in obesity-induced stress responses, but the possible link between Pdcd4 and SGs remains lacking. In this study we showed that oxidized low-density lipoprotein (ox-LDL) or high-fat diet (HFD) induced SG formation in mouse macrophages and liver tissues, and Pdcd4 deficiency in mice remarkably reduced its formation. In response to ox-LDL, either endogenous or ectopic Pdcd4 displayed granule-like expression and co-localized with SG markers including T-cell-restricted intracellular antigen-1, fragile X mental retardation-related protein 1, and eukaryotic initiation factor 4A...
2016: PloS One
Haiou Yang, Zhaojing Zheng, Haiqing Cai, Huimin Li, Xingchen Ye, Xiaoqing Zhang, Zhigang Wang, Qihua Fu
Congenital talipes equinovarus (CTEV) is one of the most common musculoskeletal disorders. Genetic factors have been suggested to be an important contributor to its pathogenesis. Some genes, including PITX1, TBX4, and RBM10, have been associated with CTEV. We aimed to determine the disease-causing mutations in Chinese patients with isolated CTEV. Genomic DNA was extracted from peripheral blood samples of a three-generation pedigree and 53 sporadic patients with CTEV. Whole-exome sequencing and Sanger sequencing were used to identify and validate disease-causing mutations, respectively...
October 2016: Human Genetics
Meihua Su, Duoduo Yang, Zhaojing Chen, Charity Cavazos, Debra A Bemben, Michael G Bemben
No abstract text is available yet for this article.
May 2016: Medicine and Science in Sports and Exercise
Zhaojing Chen, Pragya Sharma-Ghimire, Daeyeol Kim, Charity Cavazos, Michael G Bemben, Debra A Bemben
No abstract text is available yet for this article.
May 2016: Medicine and Science in Sports and Exercise
Prakash Babu Narasimhan, Sasisekhar Bennuru, Zhaojing Meng, Rachel N Cotton, Kathleen R Elliott, Sundar Ganesan, Renee McDonald-Fleming, Timothy D Veenstra, Thomas B Nutman, Roshanak Tolouei Semnani
Immune modulation is a hallmark of patent filarial infection, including suppression of antigen-presenting cell function and downmodulation of filarial antigen-specific T cell responses. The mammalian target of rapamycin (mTOR) signaling pathway has been implicated in immune regulation, not only by suppressing T cell responses but also by regulating autophagy (through mTOR sensing amino acid availability). Global proteomic analysis (liquid chromatography-tandem mass spectrometry) of microfilaria (mf)-exposed monocyte-derived dendritic cells (DC) indicated that multiple components of the mTOR signaling pathway, including mTOR, eIF4A, and eIF4E, are downregulated by mf, suggesting that mf target this pathway for immune modulation in DC...
September 2016: Infection and Immunity
Zhaojing Jiang, Qiancheng Song, Rong Zeng, Jing Li, Jingyu Li, Xiaochun Lin, Xing Chen, Jiren Zhang, Yanfang Zheng
Repeated infection with high-risk HPV is a major cause for the development and metastasis of human cervical cancer, even though the mechanism of the metastasis is still not completely understood. Here, we reported that miR-218 (microRNA-218) was downregulated in cervical cancer tissues, especially in metastatic cancer tissues. We found that miR-218 expression was associated with clinicopathological characteristics of patients with cervical cancer. MiR-218 overexpression inhibited Epithelial-Mesenchymal Transition (EMT), migration and invasiveness of cervical cancer cells in vitro...
July 19, 2016: Oncotarget
Guolin You, Haiqing Cai, Limin Jiang, Zhaojing Zheng, Bo Wang, Qihua Fu, Jing Wang
No abstract text is available yet for this article.
August 1, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
Wenli Shen, Yuanyuan Qu, Xiaofang Pei, Xuwang Zhang, Qiao Ma, Zhaojing Zhang, Shuzhen Li, Jiti Zhou
OBJECTIVE: To investigate green synthesis of gold nanoparticles (AuNPs) by Trichosporon montevideense, and to study their reduction of nitroaromatics. RESULTS: AuNPs had a characteristic absorption maximum at 535 nm. Scanning electron microscopy images revealed that the biosynthesized nanoparticles were attached on the cell surface. X-ray diffraction analysis indicated that the particles formed as face-centered cubic (111)-oriented crystals. The average size of AuNPs decreased from 53 to 12 nm with increasing biomass concentration...
September 2016: Biotechnology Letters
Dianhui Luo, Xiumei Yuan, Yawei Zeng, Kaiying Nie, Zhiming Li, Zhaojing Wang
A crude polysaccharide was extracted from the edible algae S. thunbergii. DEAE-Sepharose CL-6B column chromatography was used to separate and purify a major polysaccharide STP-II (63.75%) from the crude polysaccharide. STP-II was found to be a homogeneous polysaccharide with a single peak by high-performance size-exclusion chromatography with a Sugar KS-804 column, have a molecular weight of 550 kD, and consist mainly of fucose, xylose, galactose, glucose and glucuronic acid. The structural assignment of STP-II was carried out using Fourier transform infrared spectroscopy analysis, periodate oxidation-smith degradation, partial hydrolysis with acid, methylation analysis and nuclear magnetic resonance studies, and the repeating unit of STP-II was thus determined...
June 5, 2016: Carbohydrate Polymers
Jiuxiang Wang, Ying Liu, Fei Liu, Changzheng Huang, Shanshan Han, Yuexia Lv, Chun-Jie Liu, Su Zhang, Yayun Qin, Lei Ling, Meng Gao, Shanshan Yu, Chang Li, Mi Huang, Shengjie Liao, Xuebin Hu, Zhaojing Lu, Xiliang Liu, Tao Jiang, Zhaohui Tang, Huiping Zhang, An-Yuan Guo, Mugen Liu
Disseminated superficial porokeratosis (DSP) is a rare keratinization disorder of the epidermis. It is characterized by keratotic lesions with an atrophic center encircled by a prominent peripheral ridge. We investigated the genetic basis of DSP in two five-generation Chinese families with members diagnosed with DSP. By whole-exome sequencing, we sequencing identified a nonsense variation c.412C > T (p.Arg138*) in the phosphomevalonate kinase gene (PMVK), which encodes a cytoplasmic enzyme catalyzing the conversion of mevalonate 5-phosphate to mevalonate 5-diphosphate in the mevalonate pathway...
2016: Scientific Reports
Yunfang Meng, Chao Zhang, Jiu Yi, Zhaojing Zhou, Zhenzong Fa, Jingyu Zhao, Yali Yang, Wei Fang, Yan Wang, Wan-Qing Liao
Cryptococcus gattii is a resurgent fungal pathogen that primarily infects immunocompetent hosts. Thus, it poses an increasingly significant impact on global public health; however, the mechanisms underlying its pathogenesis remain largely unknown. We conducted a detailed characterization of the deubiquitinase Ubp5 in the biology and virulence of C. gattii using the hypervirulent strain R265, and defined its properties as either distinctive or shared with C. neoformans. Deletion of the C. gattii Ubp5 protein by site-directed disruption resulted in a severe growth defect under both normal and stressful conditions (such as high temperature, high salt, cell wall damaging agents, and antifungal agents), similar to the effects observed in C...
2016: PloS One
Tomoyasu Sugiyama, Gobi Thillainadesan, Venkata R Chalamcharla, Zhaojing Meng, Vanivilasini Balachandran, Jothy Dhakshnamoorthy, Ming Zhou, Shiv I S Grewal
Erh1, the fission yeast homolog of Enhancer of rudimentary, is implicated in meiotic mRNA elimination during vegetative growth, but its function is poorly understood. We show that Erh1 and the RNA-binding protein Mmi1 form a stoichiometric complex, called the Erh1-Mmi1 complex (EMC), to promote meiotic mRNA decay and facultative heterochromatin assembly. To perform these functions, EMC associates with two distinct complexes, Mtl1-Red1 core (MTREC) and CCR4-NOT. Whereas MTREC facilitates assembly of heterochromatin islands coating meiotic genes silenced by the nuclear exosome, CCR4-NOT promotes RNAi-dependent heterochromatin domain (HOOD) formation at EMC-target loci...
March 3, 2016: Molecular Cell
Papa M Drame, Zhaojing Meng, Sasisekhar Bennuru, Jesica A Herrick, Timothy D Veenstra, Thomas B Nutman
UNLABELLED: Immunoassays are currently needed to quantify Loa loa microfilariae (mf). To address this need, we have conducted proteomic and bioinformatic analyses of proteins present in the urine of a Loa mf-infected patient and used this information to identify putative biomarkers produced by L. loa mf. In total, 70 of the 15,444 described putative L. loa proteins were identified. Of these 70, 18 were L. loa mf specific, and 2 of these 18 (LOAG_16297 and LOAG_17808) were biologically immunogenic...
February 16, 2016: MBio
Pu Wang, Qinghua Zhang, Yingming Li, Chaofei Zhu, Zhaojing Chen, Shucheng Zheng, Huizhong Sun, Yong Liang, Guibin Jiang
No abstract text is available yet for this article.
2015: Scientific Reports
Qiao Ma, Yuanyuan Qu, Xuwang Zhang, Ziyan Liu, Huijie Li, Zhaojing Zhang, Jingwei Wang, Wenli Shen, Jiti Zhou
Indole is widely spread in various environmental matrices. Indole degradation by bacteria has been reported previously, whereas its degradation processes driven by aerobic microbial community were as-yet unexplored. Herein, eight sequencing batch bioreactors fed with municipal and coking activated sludges were constructed for aerobic treatment of indole. The whole operation processes contained three stages, i.e. stage I, glucose and indole as carbon sources; stage II, indole as carbon source; and stage III, indole as carbon and nitrogen source...
December 11, 2015: Scientific Reports
Xiumei Yuan, Yawei Zeng, Kaiying Nie, Dianhui Luo, Zhaojing Wang
Sargassum thunbergii is a kind of natural edible algae. STP (S. thunbergii polysaccharides) was considered as the main bioactive compounds in S. thunbergii. To obtain the optimal processing conditions for maximum total sugar yield, single factor investigation and response surface methodology (RSM) were employed. The optimal processing conditions were as follows: liquid to solid ratio 120 mL/g, extraction time 210 min, extraction temperature 97°C. The experimental yield 7.53% under optimized conditions was closely agreed with the predicted yield 7...
2015: PloS One
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