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https://www.readbyqxmd.com/read/27911383/parallel-measurement-of-circadian-clock-gene-expression-and-hormone-secretion-in-human-primary-cell-cultures
#1
Volodymyr Petrenko, Camille Saini, Laurent Perrin, Charna Dibner
Circadian clocks are functional in all light-sensitive organisms, allowing for an adaptation to the external world by anticipating daily environmental changes. Considerable progress in our understanding of the tight connection between the circadian clock and most aspects of physiology has been made in the field over the last decade. However, unraveling the molecular basis that underlies the function of the circadian oscillator in humans stays of highest technical challenge. Here, we provide a detailed description of an experimental approach for long-term (2-5 days) bioluminescence recording and outflow medium collection in cultured human primary cells...
November 11, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27910074/evolutional-characterization-of-photochemically-induced-stroke-in-rats-a-multimodality-imaging-and-molecular-biological-study
#2
Nai-Wei Liu, Chien-Chih Ke, Yonghua Zhao, Yi-An Chen, Kim-Chuan Chan, David Tat-Wei Tan, Jhih-Shian Lee, You-Yin Chen, Tun-Wei Hsu, Ya-Ju Hsieh, Chi-Wei Chang, Bang-Hung Yang, Wen-Sheng Huang, Ren-Shyan Liu
Photochemically induced cerebral ischemia is an easy-manipulated, reproducible, relatively noninvasive, and lesion controllable model for translational study of ischemic stroke. In order to longitudinally investigate the characterization of the model, magnetic resonance imaging, (18)F-2-deoxy-glucose positron emission tomography, fluorescence, and bioluminescence imaging system were performed in correlation with triphenyl tetrazolium chloride (TTC), hematoxylin-eosin staining, and immunohistochemistry examinations of glial fibrillary acidic protein, CD68, NeuN, von willebrand factor, and α-smooth muscle actin in the infarct zone...
December 1, 2016: Translational Stroke Research
https://www.readbyqxmd.com/read/27910052/a-needleless-liquid-jet-injection-delivery-approach-for-cardiac-gene-therapy
#3
Anthony S Fargnoli, Michael G Katz, Charles R Bridges
Fundamentally, cardiac gene therapy clinical trials have demonstrated that route efficiency is paramount in achieving maximum myocardial expression within safety limits. Gene transfer phenomena are largely influenced by physical transport principles (i.e., pressure, residence time, dispersion trafficking, mechanical resistance) that are independent of therapeutic characteristics. An alternative to intracoronary infusion methods, in an effort to improve efficiency in terms of cardiac specificity, is direct myocardial delivery via surgical injection...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27909226/unique-cytokine-and-chemokine-responses-to-exertional-heat-stroke-in-mice
#4
Michelle A King, Lisa R Leon, Deborah A Morse, Thomas L Clanton
In heat stroke, cytokines are believed to play important roles in multi-organ dysfunction and recovery of damaged tissue. The time course of the cytokine response is well defined in passive heat stroke (PHS), but little is known about exertional heat stroke (EHS). In this study we used a recently developed mouse EHS model to measure the responses of circulating cytokines/chemokines and cytokine gene expression in muscle. A very rapid increase in circulating IL-6 was observed at max core temperature (Tc,max) that peaked at 0...
December 1, 2016: Journal of Applied Physiology
https://www.readbyqxmd.com/read/27906129/the-evolutionary-origin-of-bilaterian-smooth-and-striated-myocytes
#5
Thibaut Brunet, Antje Hl Fischer, Patrick Rh Steinmetz, Antonella Lauri, Paola Bertucci, Detlev Arendt
The dichotomy between smooth and striated myocytes is fundamental for bilaterian musculature, but its evolutionary origin is unsolved. In particular, interrelationships of visceral smooth muscles remain unclear. Absent in fly and nematode, they have not yet been characterized molecularly outside vertebrates. Here, we characterize expression profile, ultrastructure, contractility and innervation of the musculature in the marine annelid Platynereis dumerilii and identify smooth muscles around the midgut, hindgut and heart that resemble their vertebrate counterparts in molecular fingerprint, contraction speed, and nervous control...
December 1, 2016: ELife
https://www.readbyqxmd.com/read/27904218/core-decompression-or-quadratus-femoris-muscle-pedicle-bone-grafting-for-nontraumatic-osteonecrosis-of-the-femoral-head-a-randomized-control-study
#6
Deqiang Li, Ming Li, Peilai Liu, Yuankai Zhang, Liang Ma, Fei Xu
BACKGROUND: The traditional management for osteonecrosis of the femoral head (ONFH) includes core decompression (CD) and quadratus femoris muscle pedicle bone graft (QF-MPBG). The aim of this study was to investigate the effects of CD and QF-MPBG on the patients with nontraumatic ONFH in an early stage. MATERIALS AND METHODS: 39 patients (47 hips) with ONFH in an early stage (Ficat Stage I or II) were randomly divided into two groups according to random number table method...
November 2016: Indian Journal of Orthopaedics
https://www.readbyqxmd.com/read/27903515/passive-heat-acclimation-improves-skeletal-muscle-contractility-in-humans
#7
Sebastien Racinais, Mathew G Wilson, Julien D Périard
The aim of this study was to investigate the effect of repeated passive heat exposure (i.e. acclimation) on muscle contractility in humans. Fourteen non-heat acclimated males completed two trials including electrically evoked twitches and voluntary contractions in thermoneutral conditions (COOL: 24ºC, 40% RH) and hot ambient conditions in the hyperthermic state (HOT: 44-50ºC, 50% RH) on consecutive days in a counterbalanced order. Rectal temperature was ~36.5ºC in COOL and was maintained at ~39ºC throughout HOT...
November 30, 2016: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/27898853/cookery-method-and-endpoint-temperature-can-affect-the-warner-bratzler-shear-force-cooking-loss-and-internal-cooked-color-of-beef-semimembranosus-and-infraspinatus-steaks
#8
J W S Yancey, J K Apple, M D Wharton
Steaks from USDA Select inside rounds (Exp. 1) and shoulder clods (Exp. 2) were used to test the interactive effect of cookery method and endpoint temperature on Warner-Bratzler shear force (WBSF) and internal cooked color. Pairs of 2.5-cm-thick semimembranosus (SM) or infraspinatus (INF) steaks ( = 360/muscle) were cut from each subprimal, labeled, vacuum packaged, and frozen at -30°C in the dark for approximately 60 d before being cooked to 65.5, 71.1, or 76.6°C using 1) a forced-air convection oven (FAC); 2) a forced-air impingement oven (IMP); 3) a gas-fired, open-hearth charbroiler (CHAR); 4) an electric countertop griddle (GRID); or 5) a clam-shell grill (CLAM)...
October 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27893484/effects-of-pelvic-and-core-strength-training-on-high-school-cross-country-race-times
#9
Anne W Clark, Maggie K Goedeke, Rina Namvong, Derek E Rockwell, Bryan Jason Lehecka, Robert C Manske, Barbara S Smith
Effects of pelvic and core strength training on high school cross-country race times: A randomized control trial.There is only limited research examining the effect of pelvic and core strength training on running performance. Pelvic and core muscle fatigue is believed to contribute to excess motion along frontal and transverse planes which decreases efficiency in normal sagittal plane running motions. The purpose of this study was to determine if adding a six week pelvic and core strengthening program resulted in decreased race times in high school cross-country runners...
November 16, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27878405/a-comparison-study-between-3d-t2-weighted-space-and-conventional-2d-t2-weighted-turbo-spin-echo-in-assessment-of-carotid-plaque
#10
Peng Lv, Yuanyuan Dai, Jiang Lin, Weisheng Zhang, Hao Liu, Hui Liu, Xiao Tang
The aim of this study was to compare 3D T2-weighted sampling perfection with application optimized contrast using different flip angle evolutions (T2w SPACE) with conventional 2D T2w turbo-spin echo (TSE) in plaque imaging of carotid artery. 45 patients underwent 3.0-T MRI for carotid arteries imaging. MR sequences included T2w SPACE, T2w TSE, Time of flight (TOF) and T1-weighted (T1w) TSE. The signal intensity of intra-plaque hemorrhage (IPH), lipid-rich necrotic core (LRNC), and loose matrix (LM) were measured and their contrast ratios (CRs) against adjacent muscle were calculated...
November 22, 2016: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/27874298/volitional-spine-stabilization-during-a-drop-vertical-jump-from-different-landing-heights-implications-for-anterior-cruciate-ligament-injury
#11
Ram Haddas, Troy Hooper, C Roger James, Phillip S Sizer
CONTEXT:  Volitional spine stabilization (VSS) during dynamic activities may alter trunk motion, but the role of the core musculature in positioning the trunk during landing tasks is unclear. OBJECTIVE:  To determine whether volitional core-muscle activation incorporated during a drop vertical jump alters lower extremity kinematics and kinetics, as well as trunk and lower extremity muscle activity at different landing heights. DESIGN:  Controlled laboratory study...
November 22, 2016: Journal of Athletic Training
https://www.readbyqxmd.com/read/27873104/analysis-of-specific-absorption-rate-and-internal-electric-field-in-human-biological-tissues-surrounding-an-air-core-coil-type-transcutaneous-energy-transmission-transformer
#12
Kenji Shiba, Nur Elina Binti Zulkifli, Yuji Ishioka
In this study, we analyzed the internal electric field E and specific absorption rate (SAR) of human biological tissues surrounding an air-core coil transcutaneous energy transmission transformer. Using an electromagnetic simulator, we created a model of human biological tissues consisting of a dry skin, wet skin, fat, muscle, and cortical bone. A primary coil was placed on the surface of the skin, and a secondary coil was located subcutaneously inside the body. The E and SAR values for the model representing a 34-year-old male subject were analyzed using electrical frequencies of 0...
November 21, 2016: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
https://www.readbyqxmd.com/read/27867498/bone-muscle-crosstalk-targets-muscle-regeneration-pathway-regulated-by-core-circadian-transcriptional-repressors-dec1-and-dec2
#13
REVIEW
Jeffrey P Gorski, Jeffrey L Price
Deletion of proprotein convertase Mbtps1 in bone osteocytes leads to a significant postnatal increase in skeletal muscle size and contractile function, while causing only a 25% increase in stiffness in long bones. Concerns about leakiness in skeletal muscle were discounted since Cre recombinase expression does not account for our findings, and, Mbtps1 protein and mRNA is not deleted. Interestingly, the response of normal skeletal muscle to exercise and the regenerative response of skeletal muscle to the deletion of Mbtps1 in bone share some key regulatory features including a preference for slow twitch muscle fibers...
2016: BoneKEy Reports
https://www.readbyqxmd.com/read/27863730/psoas-muscle-area-and-length-of-stay-in-older-adults-undergoing-cardiac-operations
#14
Jesse Zuckerman, Matthew Ades, Louis Mullie, Amanda Trnkus, Jean-Francois Morin, Yves Langlois, Felix Ma, Mark Levental, José A Morais, Jonathan Afilalo
BACKGROUND: Frailty assessment can help predict which older adults will experience adverse events after cardiac surgical procedures. Low muscle mass is a core component of frailty that is suboptimally captured by self-reported weight loss; refined measures using computed tomographic (CT) images have emerged and are predictive of outcomes in noncardiac surgical procedures. The objective of this study was to evaluate the association between CT muscle area and length of stay (LOS) after cardiac surgical procedures...
November 15, 2016: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/27853436/the-importance-of-intrinsically-disordered-segments-of-cardiac-troponin-in-modulating-function-by-phosphorylation-and-disease-causing-mutations
#15
REVIEW
Maria Papadaki, Steven B Marston
Troponin plays a central role in regulation of muscle contraction. It is the Ca(2+) switch of striated muscles including the heart and in the cardiac muscle it is physiologically modulated by PKA-dependent phosphorylation at Ser22 and 23. Many cardiomyopathy-related mutations affect Ca(2+) regulation and/or disrupt the relationship between Ca(2+) binding and phosphorylation. Unlike the mechanism of heart activation, the modulation of Ca(2+)-sensitivity by phosphorylation of the cardiac specific N-terminal segment of TnI (1-30) is structurally subtle and has proven hard to investigate...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27852594/hip-strength-as-an-intrinsic-risk-factor-for-lateral-ankle-sprains-in-youth-soccer-players-a-3-season-prospective-study
#16
Roel De Ridder, Erik Witvrouw, Mieke Dolphens, Philip Roosen, Ans Van Ginckel
BACKGROUND: Numerous epidemiological studies have emphasized the burden of lateral ankle sprains in youth soccer players. However, no prospective study has identified intrinsic physical and modifiable risk factors for these injuries in this particular population. Although injury prevention programs in soccer incorporate proximal hip and core stability exercises, it is striking that the relationship between impaired proximal hip function and ankle sprains has not yet been prospectively investigated in youth soccer players...
November 16, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27847720/dilemmas-of-korean-athletes-with-a-spinal-cord-injury-to-participate-in-sports-a-survey-based-on-the-icf-core-set-for-spinal-cord-injury
#17
Je Shik Nam, Ko Eun Lee, Ah Young Jun, Chong Suck Parke, Hyun Young Kim, You Hyeon Chae
OBJECTIVE: To explore the experiences of athletes with spinal cord injury (SCI) in Korea with respect to dilemmas of participating in sports with regards to the facilitators and barriers, using the International Classification of Functioning, Disability and Health (ICF). METHODS: The facilitators and barriers to sports participation of individuals with SCI were examined using 112 ICF categories. A questionnaire in dichotomous scale was answered, which covered the subjects 'Body functions', 'Body structures', 'Activity and participation' and 'Environmental factors'...
October 2016: Annals of Rehabilitation Medicine
https://www.readbyqxmd.com/read/27846435/muscle-activation-patterns-of-the-lumbo-pelvic-hip-complex-during-walking-gait-before-and-after-exercise
#18
Mihyang Chang, Lindsay V Slater, Revay O Corbett, Joseph M Hart, Jay Hertel
The lumbo-pelvic-hip core complex consists of musculoskeletal structures that stabilize the spine and pelvis, however fatigue may affect muscle recruitment, active muscle stiffness and trunk kinematics, compromising trunk stability. The purpose of this study was to compare trunk muscle activation patterns, and trunk and lower extremity kinematics during walking gait before and after exercise. Surface electrodes were placed over the rectus abdominis, external oblique, erector spinae, gluteus medius, vastus lateralis, and vastus medialis of twenty-five healthy inidviduals...
November 9, 2016: Gait & Posture
https://www.readbyqxmd.com/read/27844487/promoter-analysis-and-tissue-expression-of-the-chicken-asb15-gene
#19
Y C Wang, Z J Li, R L Han, C L Xu, S H Wang, G R Sun, S H Wang, J P Wu, X T Kang
1. This study was conducted to explore the promoter region of the chicken ASB15 gene by detecting the activities of the dual luciferase reporter gene, and to assess expression profiles of the ASB15 gene in 10 different tissues from Gushi chicken. 2. Five dual luciferase reporter gene vectors were constructed and transfected into DF1 cells. Then the activities of recombined plasmids were measured and the core promoter was confirmed by bioinformatic analysis. Total RNA was extracted and the relative expression of the ASB15 gene was examined...
November 15, 2016: British Poultry Science
https://www.readbyqxmd.com/read/27826954/critical-and-diverse-in-vivo-roles-of-apoptosis-signal-regulating-kinase-1-in-animal-models-of-atherosclerosis-and-cholestatic-liver-injury
#20
REVIEW
Sohsuke Yamada, Hirotsugu Noguchi, Akihide Tanimoto
Apoptosis plays pivotal in vivo roles in not only vital processes, such as cell turnover and embryonic development, but also various inflammatory disorders. However, the role of apoptosis by vascular and hepatic cells in the respective progression of atherosclerosis and liver injury remains controversial. Apoptosis signal-regulating kinase 1 (ASK1) is a mitogen-activated protein kinase kinase kinase family member that is activated through distinct mechanisms in response to various cytotoxic stressors. ASK1, ubiquitously expressed, is situated in an important upstream position for many signal transduction pathways, which subsequently induce inflammation and/or apoptosis...
November 9, 2016: Histology and Histopathology
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