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https://www.readbyqxmd.com/read/28720935/technological-options-to-control-quality-of-fish-burgers
#1
A Danza, A Conte, M A Del Nobile
This research was focused on preservation strategies applied to develop fish burgers enriched with tomato flour and extra-virgin olive oil. The effects of three different gas mixtures (5:95 O2/CO2; 10:60:30 O2/CO2/N2 and 5:50:45 O2/CO2/N2) on burger quality were analyzed by monitoring microbial cell load of main spoilage microorganisms, pH and sensory properties. As expected, modified atmosphere packaging significantly affected mesophilic bacteria with a reduction of about 2 log cycles for samples under 5% O2 and 95% CO2...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28712045/dynamics-of-soil-bacterial-communities-over-a-vegetation-season-relate-to-both-soil-nutrient-status-and-plant-growth-phenology
#2
Davide Francioli, Elke Schulz, François Buscot, Thomas Reitz
Soil microorganisms regulate element cycling and plant nutrition, mediate co-existence of neighbors, and stabilize plant communities. Many of these effects are dependent upon environmental conditions and, in particular, on nutrient quality and availability in soils. In this context, we set up a pot experiment in order to examine the combined effects of soil nutrient availability and microbial communities on plant-soil interactions and to investigate assemblage rules for soil bacterial communities under changed nutrient conditions...
July 15, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28711528/comparison-of-five-methods-for-isolation-of-dna-from-mycoplasma-cynos
#3
Aslı Sakmanoglu, Zafer Sayin, Uckun Sait Ucan, Yasemin Pinarkara, Ali Uslu, Osman Erganis
Extraction of DNA from Mycoplasma cultured on agar medium is difficult because the plasticity of these microorganisms enables agar penetration. This eventually causes cell loss during harvesting of colonies from the agar surface. Here, we used the GenElute™ gel extraction kit, which is usually used to purify polymerase chain reaction products, for extracting DNA from Mycoplasma. We compared the DNA extraction efficiency of the GenElute™ gel extraction kit from Mycoplasma cynos cultured in agar medium with four other DNA extraction methods...
July 12, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28703952/potential-of-bacteriocins-from-lab-to-improve-microbial-quality-of-dry-cured-and-fermented-meat-products
#4
Paulina Kęska, Joanna Stadnik, Dorota Zielińska, Danuta Kołożyn-Krajewska
Meat and meat products are an important component of the daily diet. Nevertheless, they are perishable goods and are prone to microbial contamination, which leads to an increased risk to the health of consumers as well as economic losses in the meat industry. Fermentation has been used for thousands of years to preserve meat. As a result of extensive biochemical reactions occurring in meat during fermentation and ripening, the condi- tions inhibiting the growth of pathogenic and spoilage bacteria are formed...
April 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28700973/fit-for-purpose-wastewater-treatment-testing-to-implementation-of-decision-support-tool-ii
#5
Gyan Chhipi-Shrestha, Kasun Hewage, Rehan Sadiq
This paper is the second in a series of two papers. In Paper I, a decision support tool (DST), FitWater, was developed for evaluating the potential of wastewater treatment (WWT) trains for various water reuse applications. In the present paper, the proposed DST has been tested and implemented. FitWater has been tested with several existing WWT plants in Canada and the USA, demonstrating FitWater's effectiveness in estimating life cycle cost (LCC), health risk, and energy use. FitWater has also been implemented in a newly planned neighbourhood in the Okanagan Valley (BC, Canada) by developing 12 alternative WWT trains for water reuse in lawn and public parks irrigation...
July 9, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28696049/increased-nutritional-value-in-food-crops
#6
Nieves Goicoechea, M Carmen Antolín
Modern agriculture and horticulture must combine two objectives that seem to be almost mutually exclusive: to satisfy the nutritional needs of an increasing human population and to minimize the negative impact on the environment. These two objectives are included in the Goal 2 of the 2030 Agenda for Sustainable Development of the United Nations: 'End hunger, achieve food security and improved nutrition and promote sustainable agriculture'. Enhancing the nutritional levels of vegetables would improve nutrient intake without requiring an increase in consumption...
July 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28689123/pyrolysis-of-attapulgite-clay-blended-with-yak-dung-enhances-pasture-growth-and-soil-health-characterization-and-initial-field-trials
#7
Muhammad Khalid Rafiq, Stephen D Joseph, Fei Li, Yanfu Bai, Zhanhuan Shang, Aditya Rawal, James M Hook, Paul R Munroe, Scott Donne, Sara Taherymoosavi, David R G Mitchell, Ben Pace, Mohanad Mohammed, Joseph Horvat, Christopher E Marjo, Avital Wagner, Yanlong Wang, Jun Ye, Rui-Jun Long
Recent studies have shown that the pyrolysis of biomass combined with clay can result in both lower cost and increase in plant yields. One of the major sources of nutrients for pasture growth, as well as fuel and building materials in Tibet is yak dung. This paper reports on the initial field testing in a pasture setting in Tibet using yak dung, biochar, and attapulgite clay/yak dung biochars produced at ratios of 10/90 and 50/50 clay to dung. We found that the treatment with attapulgite clay/yak dung (50/50) biochar resulted in the highest pasture yields and grass nutrition quality...
July 12, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28688926/microbes-from-mined-sites-harnessing-their-potential-for-reclamation-of-derelict-mine-sites
#8
REVIEW
Palanisami Thavamani, R Amos Samkumar, Viswanathan Satheesh, Suresh R Subashchandrabose, Kavitha Ramadass, Ravi Naidu, Kadiyala Venkateswarlu, Mallavarapu Megharaj
Derelict mines pose potential risks to environmental health. Several factors such as soil structure, organic matter, and nutrient content are the greatly affected qualities in mined soils. Soil microbial communities are an important element for successful reclamation because of their major role in nutrient cycling, plant establishment, geochemical transformations, and soil formation. Yet, microorganisms generally remain an undervalued asset in mined sites. The microbial diversity in derelict mine sites consists of diverse species belonging to four key phyla: Proteobacteria, Acidobacteria, Firmicutes, and Bacteroidetes...
July 6, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28688187/reexamining-opportunities-for-therapeutic-protein-production-in-eukaryotic-microorganisms
#9
REVIEW
M Catherine Bartlett, Chapman Wright, Angel Kuo, Noelle Colant, Matthew Westoby, J Christopher Love
Antibodies are an important class of therapeutics and are predominantly produced in Chinese Hamster Ovary (CHO) cell lines. While this manufacturing platform is sufficiently productive to supply patient populations of currently approved therapies, it is unclear whether or not the current CHO platform can address two significant areas of need: affordable access to biologics for patients around the globe and production of unprecedented quantities needed for very large populations of patients. Novel approaches to recombinant protein production for therapeutic biologic products may be needed, and might be enabled by non-mammalian expression systems and recent advances in bioengineering...
July 8, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28687514/management-and-control-of-microbiologically-influenced-corrosion-mic-in-the-oil-and-gas-industry-overview-and-a-north-sea-case-study
#10
Torben Lund Skovhus, Richard B Eckert, Edgar Rodrigues
Microbiologically influenced corrosion (MIC) is the terminology applied where the actions of microorganisms influence the corrosion process. In literature, terms such as microbial corrosion, biocorrosion, microbially influenced/induced corrosion, and biodegradation are often applied. MIC research in the oil and gas industry has seen a revolution over the past decade, with the introduction of molecular microbiological methods: (MMM) as well as new industry standards and procedures of sampling biofilm and corrosion products from the process system...
July 4, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28687186/evaluation-of-a-new-packaging-process-for-non-autoclavable-endoscopes-results-for-the-first-100-microbiological-samples
#11
C Minebois, P Saviuc, J Shum, P Tuvignon, I Pelloux, M-P Brenier-Pinchart, C Landelle, M-R Mallaret
BACKGROUND: A new process for packaging endoscopes (SureStore(®), Medical Innovations Group) immediately after they exit from washing and disinfection in an automated endoscope reprocessor (AER) allows for endoscopes to be stored for up to 15 days. AIM: To describe the microbiological quality of samples from gastrointestinal endoscopes following this process. METHODS: Three-month prospective study using microbiological sampling from a stock of 38 gastrointestinal endoscopes carried out in a French University Hospital...
July 4, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28678713/genome-wide-analysis-of-mdr-and-xdr-tuberculosis-from-belarus-machine-learning-approach
#12
Roman Sergeevich Sergeev, Ivan Kavaliou, Uladzislau Sataneuski, Andrei Gabrielian, Alex Rosenthal, Michael Tartakovsky, Alexander Tuzikov
Emergence of drug-resistant microorganisms has been recognized as a serious threat to public health worldwide. This problem is extensively discussed in the context of tuberculosis treatment. Alterations in pathogen genomes are among the main mechanisms by which microorganisms exhibit drug resistance. Analysis of 144 M. tuberculosis strains of different phenotypes including drug susceptible, MDR and XDR isolated in Belarus was fulfilled in this paper. A wide range of machine learning methods that can discover SNPs related to drug-resistance in the whole bacteria genomes was investigated...
June 27, 2017: IEEE/ACM Transactions on Computational Biology and Bioinformatics
https://www.readbyqxmd.com/read/28678529/microbial-community-biomass-and-structure-in-saline-and-non-saline-soils-associated-with-salt-boron-tolerant-poplar-clones-grown-for-the-phytoremediation-of-selenium
#13
Sadikshya R Dangi, Gary Bañuelos, Jeffrey S Buyer, Bradley Hanson, James Gerik
Poplar trees (Populus spp.) are often used in bioremediation strategies because of their ability to phytoextract potential toxic ions, e.g., selenium (Se) from poor quality soils. Soil microorganisms may play a vital role in sustaining health of soil and/or tolerance of these trees grown in poor quality soils by contributing to nutrient cycling, soil structure, overall soil quality and plant survival. The effect of naturally-occurring salts, boron (B) and Se on soil microbial community composition associated with poplar trees is not known for bioremediation strategies...
July 5, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28675994/effect-of-treatment-with-caspofungin-on-the-diagnosis-of-fungal-infections-by-gamma-scintigraphy
#14
L Fernández, A L Reyes, P Oliver, M Terán
The aim of this work is to assess the influence of treatment with unlabeled-Caspofungin, on the quality of diagnostic scintigraphic images of induced lesions in nude mice undergoing both bacterial and fungal infections and to determine the level of specificity of 99mTc-tricarbonyl-Caspofungin to discriminate between fungal or bacterial infections. In vitro studies on the behaviour of the 99mTc-tricarbonyl-Caspofungin complex binding percentage at different yeast concentrations of Candida albicans or Staphylococcus aureus were made...
July 3, 2017: Current Radiopharmaceuticals
https://www.readbyqxmd.com/read/28669799/comparison-of-dna-extraction-methods-used-to-detect-bacterial-and-yeast-dna-from-spiked-whole-blood-by-real-time-pcr
#15
Libera M Dalla-Costa, Luis G Morello, Danieli Conte, Luciane A Pereira, Jussara K Palmeiro, Altair Ambrosio, Dayane Cardozo, Marco A Krieger, Sonia M Raboni
Sepsis is the leading cause of death in intensive care units (ICUs) worldwide and its diagnosis remains a challenge. Blood culturing is the gold standard technique for blood stream infection (BSI) identification. Molecular tests to detect pathogens in whole blood enable early use of antimicrobials and affect clinical outcomes. Here, using real-time PCR, we evaluated DNA extraction using seven manual and three automated commercially available systems with whole blood samples artificially contaminated with Escherichia coli, Staphylococcus aureus, and Candida albicans, microorganisms commonly associated with BSI...
June 29, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28667638/organic-carbon-and-nitrogen-availability-determine-bacterial-community-composition-in-paddy-fields-of-the-indo-gangetic-plain
#16
Arvind Kumar, Lal Chand Rai
Soil quality is an important factor and maintained by inhabited microorganisms. Soil physicochemical characteristics determine indigenous microbial population and rice provides food security to major population of the world. Therefore, this study aimed to assess the impact of physicochemical variables on bacterial community composition and diversity in conventional paddy fields which could reflect a real picture of the bacterial communities operating in the paddy agro-ecosystem. To fulfill the objective; soil physicochemical characterization, bacterial community composition and diversity analysis was carried out using culture-independent PCR-DGGE method from twenty soils distributed across eight districts...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28666419/effectiveness-of-atp-bioluminescence-assay-for-presumptive-identification-of-microorganisms-in-hospital-water-sources
#17
Máira Gazzola Arroyo, Adriano Menis Ferreira, Oleci Pereira Frota, Marcelo Alessandro Rigotti, Denise de Andrade, Natalia Seron Brizzotti, Jacqueline Tanury Macruz Peresi, Elza Maria Castilho, Margarete Teresa Gottardo de Almeida
BACKGROUND: Laboratory analysis of organisms in water include arduous methods, such as the multiple tube and membrane filter. The ATP bioluminescence system, proposes a new way of measuring cellular material in water by measuring adenosine triphosphate (ATP) levels, which are expressed in relative light units (RLU). The ATP bioluminescence assay has been increasingly used to assess the microbiological safety of the hospital environment. However, there are few studies investigating the use of this methodology to evaluate the microbiological quality of water...
June 30, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28663877/subsampled-phase-retrieval-for-temporal-resolution-enhancement-in-lensless-on-chip-holographic-video
#18
Donghun Ryu, Zihao Wang, Kuan He, Guoan Zheng, Roarke Horstmeyer, Oliver Cossairt
On-chip holographic video is a convenient way to monitor biological samples simultaneously at high spatial resolution and over a wide field-of-view. However, due to the limited readout rate of digital detector arrays, one often faces a tradeoff between the per-frame pixel count and frame rate of the captured video. In this report, we propose a subsampled phase retrieval (SPR) algorithm to overcome the spatial-temporal trade-off in holographic video. Compared to traditional phase retrieval approaches, our SPR algorithm uses over an order of magnitude less pixel measurements while maintaining suitable reconstruction quality...
March 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28660454/an-improved-method-suitable-for-isolation-of-high-quality-metagenomic-dna-from-diverse-soils
#19
Sumit Kumar Verma, Himanshi Singh, Prakash C Sharma
Standardization of metagenomic DNA extraction protocol is a pre-requisite for a successful metagenomic study aiming to screen and exploit the variety of microorganisms inhabiting a particular soil environment. Six methods reported earlier were used for isolation of metagenomic DNA in the present study. These methods suffered with regard to either poor yield or quality of DNA. Therefore, we developed an improved method for isolation of high-molecular weight and good quality metagenomic DNA from different soil samples...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28659558/biopreservative-efficacy-of-bacteriocin-bacfl31-in-raw-ground-turkey-meat-in-terms-of-microbiological-physicochemical-and-sensory-qualities
#20
Ahlem Chakchouk-Mtibaa, Slim Smaoui, Naourez Ktari, Imen Sellem, Soumaya Najah, Ines Karray-Rebai, Lotfi Mellouli
 The effect of the semi purified bacteriocin BacFL31 at 200 and 400 AU/g on the shelf life of refrigerated raw ground turkey meat was investigated. The microbiological, physicochemical, and sensory properties of the meat samples were examined during refrigerated storage. The findings indicated that BacFL31 treatments were effective (p<0.05) against the proliferation of various spoilage microorganisms and suppressed the growth of Listeria monocytogenes and Salmonella Typhimurium. The pH, % Met-MB, and TBA-RS values of the treated samples were lower (p<0...
2017: Biocontrol Science
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