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Microorganism quality

Seung Jae Lee, Seung Yun Lee, Gap Don Kim, Geun Bae Kim, Sang Keun Jin, Sun Jin Hur
BACKGROUND: Active packaging refers to the mixing of additive agents into packagingmaterials with the purpose of hold or extending food product quality and shelf life. The purpose of this study was to develop an easy and cheap active packaging in beef. Beef loin samples were divided into 3 packaging groups (C: ziplock bag-packaging, T1: vacuum-packaging, T2: active-packaging) and stored at 4 °C for 21 days. RESULTS: The water-holding capacity was significantly (p < 0...
January 20, 2017: Journal of the Science of Food and Agriculture
Antonio Higinio Sánchez, Antonio López-López, Víctor Manuel Beato, Antonio de Castro, Alfredo Montaño
BACKGROUND: There is an increasing interest in the use of pasteurizable plastic packaging by the olive industry. In order to investigate the change from traditional glass or varnished can containers to plastic packaging, the proper plastic material that is compatible with fermented olives while maintaining color quality during pasteurization treatment and storage must be selected. This work is focused on color stability in two distinct pasteurizable plastic containers with different oxygen permeability...
January 19, 2017: Journal of the Science of Food and Agriculture
John Vollmers, Sandra Wiegand, Anne-Kristin Kaster
With the constant improvement in cost-efficiency and quality of Next Generation Sequencing technologies, shotgun-sequencing approaches -such as metagenomics- have nowadays become the methods of choice for studying and classifying microorganisms from various habitats. The production of data has dramatically increased over the past years and processing and analysis steps are becoming more and more of a bottleneck. Limiting factors are partly the availability of computational resources, but mainly the bioinformatics expertise in establishing and applying appropriate processing and analysis pipelines...
2017: PloS One
Vesela Georgieva, Yulia Dimitrova
BACKGROUND: Due to the widespread use of bottled water, it is important from a hygienic point of view to ensure that these products have good quality and meet the requirements set out in regulations. METHODS: In the period 2000-2013, a large-scale study (2,500 samples) was carried out on the quality of Bulgarian bottled water as per the microbiological parameters laid down in the European and Bulgarian legislation. Standard microbiological methods were applied. RESULTS: There is strong evidence that the most frequently isolated contaminant in these products was Pseudomonas aeruginosa...
December 2016: Central European Journal of Public Health
Xiao-Ming Lu, Peng-Zhen Lu, Ke Yang
Microbial groups are major factors that influence soil function. Currently, there is a lack of studies on microbial functional groups. Although soil microorganisms play an important role in the nitrogen cycle, systematic studies of the effects of environmental factors on microbial populations in relation to key metabolic processes in the nitrogen cycle are seldom reported. In this study, we conducted a systematic analysis of the changes in nitrogen functional groups in mandarin orange garden soil treated with Azolla imbricata...
January 16, 2017: Applied Microbiology and Biotechnology
Carmen Astudillo-Garcia, James J Bell, Nicole S Webster, Bettina Glasl, Jamaluddin Jompa, Jose M Montoya, Michael W Taylor
The study of complex microbial communities poses unique conceptual and analytical challenges, with microbial species potentially numbering in the thousands. With transient or allochthonous microorganisms often adding to this complexity, a 'core' microbiota approach, focusing only on the stable and permanent members of the community, is becoming increasingly popular. Given the various ways of defining a core microbiota, it is prudent to examine whether the definition of the core impacts upon the results obtained...
January 12, 2017: Environmental Microbiology
Nakarin Suwannarach, Chariya Kaewyana, Arpaporn Yodmeeklin, Jaturong Kumla, Kenji Matsui, Saisamorn Lumyong
The presence of microorganisms on the eggshell surface is a factor of consideration in determining egg quality. These microorganisms can contribute to egg spoilage and can infect the egg. In this study, 18 morphotypes of microorganisms were isolated from eggshells. Morphological, biochemical, physiological and molecular analyses were used to identify these morphotypes into 7 species; Bacillus drentensis, Staphylococcus arlettae, Stap. cohnii, Stap. kloosii, Stap. saprophyticus, Stap. sciuri and Stap. xylosus...
December 28, 2016: International Journal of Food Microbiology
Daniel Wasilkowski, Anna Nowak, Grażyna Płaza, Agnieszka Mrozik
The objective of this study was to explore the potential use of pulp (by-product) from coffee processing and Na-bentonite (commercial product) for minimizing the environmental risk of Zn, Pb and Cd in soil collected from a former mine and zinc-lead smelter. The effects of soil amendments on the physicochemical properties of soil, the structural and functional diversity of the soil microbiome as well as soil enzymes were investigated. Moreover, biomass of Festuca arundinacea Schreb. (cultivar Asterix) and the uptake of trace elements in plant tissues were studied...
2017: PloS One
Stanislava Voběrková, Magdalena D Vaverková, Alena Burešová, Dana Adamcová, Martina Vršanská, Jindřich Kynický, Martin Brtnický, Vojtěch Adam
An investigation was carried out on the effect of inoculation methods on the compost of an organic fraction of municipal solid waste. Three types of white-rot fungi (Phanerochaete chrysosporium, Trametes versicolor and Fomes fomentarius), and a consortium of these fungi, were used. The study assessed their influence on microbial enzymatic activities and the quality of the finished compost. It was found that the addition of white-rot fungi to municipal solid waste (after 37days of composting) could be a useful strategy for enhancing the properties of the final compost product...
January 5, 2017: Waste Management
Mutshiene Deogratias Ekwanzala, Akebe Luther King Abia, Eunice Ubomba-Jaswa, Jitendra Keshri, Ndombo Benteke Maggy Momba
To date, the microbiological quality of river sediments and its impact on water resources are not included in the water quality monitoring assessment. Therefore, the aim of this study was to establish genetic relatedness between faecal coliforms and enterococci isolated from the river water and riverbed sediments of Apies River to better understand the genetic similarity of microorganisms between the sediment and water phases. Indicator bacteria were subjected to a molecular study, which consisted of PCR amplification and sequence analysis of the 16S rRNA and 23S rRNA gene using specific primers for faecal coliforms and enterococci, respectively...
December 2017: AMB Express
Nithya Babu Rajendran, Beryl Primrose Gladstone, Jesús Rodríguez-Baño, Frangiscos Sifakis, Andreas Voss, Yehuda Carmeli, Francesco Robert Burkert, Panagiota Gkolia, Evelina Tacconelli
INTRODUCTION: Improving our understanding of outbreaks due to antibiotic-resistant bacteria (ARB) and their control is critical in the current public health scenario. The threat of outbreaks due to ARB requires multifaceted efforts. However, a global overview of epidemiological characteristics of outbreaks due to ARB and effective infection control measures is missing. In this paper, we describe the protocol of a systematic review aimed at mapping and characterising the epidemiological aspects of outbreaks due to ARB and infection control measures in European countries...
5, 2017: BMJ Open
Linhuan Wu, Qinglan Sun, Philippe Desmeth, Hideaki Sugawara, Zhenghong Xu, Kevin McCluskey, David Smith, Vasilenko Alexander, Nelson Lima, Moriya Ohkuma, Vincent Robert, Yuguang Zhou, Jianhui Li, Guomei Fan, Supawadee Ingsriswang, Svetlana Ozerskaya, Juncai Ma
The World Data Centre for Microorganisms (WDCM) was established 50 years ago as the data center of the World Federation for Culture Collections (WFCC)-Microbial Resource Center (MIRCEN). WDCM aims to provide integrated information services using big data technology for microbial resource centers and microbiologists all over the world. Here, we provide an overview of WDCM including all of its integrated services. Culture Collections Information Worldwide (CCINFO) provides metadata information on 708 culture collections from 72 countries and regions...
January 4, 2017: Nucleic Acids Research
V Alonso, L Cavaglieri, A J Ramos, A Torres, S Marin
AIMS: The aim of this work was to use mathematical kinetic modelling to assess the combined effects of aW, pH, O2 availability and temperature onto the growth rate and time to growth of A. fumigatus strains isolated from corn silage. METHODS AND RESULTS: A full factorial design was used in which two factors were assayed: pH and aW . The aW levels assayed were 0.80, 0.85, 0.90, 0.92, 0.94, 0.96, 0.98 and 0.99. Levels of pH assayed were 3.5, 4, 4.5, 5, 6, 7, 7.5, and 8...
January 4, 2017: Journal of Applied Microbiology
Guang-Yu Wang, Hu-Hu Wang, Yi-Wei Han, Tong Xing, Ke-Ping Ye, Xing-Lian Xu, Guang-Hong Zhou
Microorganisms play an important role in the spoilage of chilled chicken. In this study, a total of 53 isolates, belonging to 7 species of 3 genera, were isolated using a selective medium based on the capacity to spoil chicken juice. Four isolates, namely Aeromonas salmonicida 35, Pseudomonas fluorescens H5, Pseudomonas fragi H8 and Serratia liquefaciens 17, were further characterized to assess their proteolytic activities in vitro using meat protein extracts and to evaluate their spoilage potential in situ...
May 2017: Food Microbiology
Elżbieta Piątkowska, Jolanta Rusiecka-Ziółkowska
Essential oils are substances of plant origin used to produce cosmetics, to preserve and aromatize food. Recently, they have become more popular among scientists and doctors due to their germicidal, antifungal, antiviral and anti-parasitic properties. As a consequence, essential oils are regarded as the source of new therapeutic substances. Numerous publications have been written regarding their effect on microorganisms in vitro. There have also been reports regarding their use in therapy as an additive to the traditional medical treatment...
September 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Mitra Arjmandi, Mariano Otón, Francisco Artés, Francisco Artés-Hernández, Perla A Gómez, Encarna Aguayo
Thermal processing extends the shelf life of fruit and vegetables products by inactivating microorganisms and enzymes. The effect of a pasteurization (P) treatment, 90 ± 2 °C for 35 s, provided by continuous semi-industrial microwave (MW) under different conditions (high power/short time and low power/long time) or conventional pasteurization (CP) on orange-colored smoothies and their changes throughout 45 days of storage at 5 °C were investigated. Results indicated that vitamin C and antioxidant capacity (FRAP) in CP decreased dramatically in comparison with the unheated and MWP smoothies...
October 2016: Journal of Food Science and Technology
Xiyang Dong, Johannes Dröge, Christine von Toerne, Sviatlana Marozava, Alice C McHardy, Rainer U Meckenstock
The enrichment culture BPL is able to degrade benzene with sulfate as electron acceptor and is dominated by an organism of the genus Pelotomaculum Members of Pelotomaculum are usually known to be fermenters, undergoing syntrophy with anaerobic respiring microorganisms or methanogens. By using a metagenomic approach, we reconstructed a high-quality genome (∼2.97 Mbp, 99% completeness) for Pelotomaculum candidate BPL. The proteogenomic data suggested: (1) anaerobic benzene degradation was activated by a yet unknown mechanism for conversion of benzene to benzoyl-CoA; (2) the central benzoyl-CoA degradation pathway involved reductive dearomatization by a class II benzoyl-CoA reductase followed by hydrolytic ring cleavage and modified β-oxidation; (3) the oxidative acetyl-CoA pathway was utilized for complete oxidation to CO2 Interestingly, the genome of Pelotomaculum candidate BPL has all the genes for a complete sulfate reduction pathway including a similar electron transfer mechanism for dissimilatory sulfate reduction as in other Gram-positive sulfate-reducing bacteria...
December 22, 2016: FEMS Microbiology Ecology
Bao-Nan He, Jiang-Tao He, Jian Wang, Jie Li, Fei Wang
The pH is a primary index reflecting water quality in rivers. The Jian River and Chaobai River are two reclaimed water intake areas which have elevated pH. This elevated pH has a marked effect on both the phytoplankton, species in water and vegetation on the shore. Understanding the main reasons causing pH elevation in river water has important implications for river ecosystem management and the improvement of water quality and can provide a theoretical basis for the direction of water quality improvement. For this reason, each biogeochemical and physical process influencing pH changes in water was quantified along the flow direction in the Wenyu to Chaobai reclaimed water diversion project, in which proton consumption and production by such processes were monitored and calculated at five monitoring sections...
December 23, 2016: Environmental Science. Processes & Impacts
Hideharu Shintani
 Water for injection (WFI) and purified water are the most widely used and stringently regulated raw material in pharmaceutical manufacturing. WFI is utilized for a parenteral drug product. Water system is monitored at frequent and routine frequency for demonstrating the overall system control and stability of performance. The critical ports demonstrating systemic control should be monitored more frequently. For reducing the overall risk of microbial contamination or microbial build-up, it is important to develop appropriate alert and action levels...
2016: Biocontrol Science
Sara Angrill, Anna Petit-Boix, Tito Morales-Pinzón, Alejandro Josa, Joan Rieradevall, Xavier Gabarrell
Rainwater harvesting might help to achieve self-sufficiency, but it must comply with health standards. We studied the runoff quantity and quality harvested from seven urban surfaces in a university campus in Barcelona according to their use (pedestrian or motorized mobility) and materials (concrete, asphalt and slabs). An experimental rainwater harvesting system was used to collect the runoff resulting from a set of rainfall events. We estimated the runoff coefficient and initial abstraction of each surface and analyzed the physicochemical and microbiological properties, and hydrocarbon and metal content of the samples...
December 18, 2016: Journal of Environmental Management
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