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https://www.readbyqxmd.com/read/28630748/treatment-of-chronic-orthopaedic-infection
#1
Heinz Winkler
Chronic infections are one of the major challenges in orthopaedic surgery, both for surgeons and patients. They are characterised by obstinate persistency of the causing microorganisms and resulting long-term disablement of the patients, associated with remarkable costs for the health care system.Difficulties derive from the biofilm-mode of living of pathogens with resistances against immunological defence and antimicrobial substances, and osseous defects resulting from the disease itself and surgical interventions...
May 2017: EFORT open reviews
https://www.readbyqxmd.com/read/28626449/characterization-of-feruloyl-esterases-produced-by-the-four-lactobacillus-species-l-amylovorus-l-acidophilus-l-farciminis-and-l-fermentum-isolated-from-ensiled-corn-stover
#2
Zhenshang Xu, Huiying He, Susu Zhang, Tingting Guo, Jian Kong
Lactic acid bacteria (LAB) play important roles in silage fermentation, which depends on the production of sufficient organic acids to inhibit the growth of undesirable microorganisms. However, LAB are not able to degrade cellulose and hemicellulose. Bacteria and fibrolytic enzymes are usually used as inoculants to improve the silage quality and digestibility. In the present study, we isolated four Lactobacillus strains (L. amylovorus CGMCC 11056, L. acidophilus CCTCC AB2010208, L. farciminis CCTCC AB2016237 and L...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28622602/assessment-of-the-phenolic-profile-antimicrobial-activity-and-oxidative-stability-of-transgenic-perilla-frutescens-l-overexpressing-tocopherol-methyltransferase-%C3%AE-tmt-gene
#3
Bimal Kumar Ghimire, Chang Yeon Yu, Ill-Min Chung
This study evaluated the effects of enhanced concentrations of α-tocopherol and phenolic compounds on the resistance and stability of Perilla oil in transgenic Perilla frutescens plants against various tested pathogenic bacteria by over-expressing the γ-tmt gene. The concentration of phenolic compounds in the non-transgenic samples was 9313.198 ± 18.887 μg g(-1) dry weight (DW), whereas the total concentration of the transgenic samples ranged from 9118.015 ± 18.822 to 10527.612 ± 20.411 μg g(-1) DW...
June 7, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28621298/is-the-stella%C3%A2-5l-system-an-effective-cold-sterilization-technique-for-needle-based-confocal-miniprobes
#4
Cai-Xia Wang, Yuan-Yuan Chen, Feng Yang, Fan Yang, Jin-Tao Guo, Siyu Sun, Ling Fan
BACKGROUND AND OBJECTIVE: Needle-based confocal laser endomicroscopy (nCLE) under endoscopic ultrasound guidance is a newly developed imaging technique for pancreatic lesions and lymph nodes, enabling a subcellular level of resolution. The confocal miniprobe is an invasive instrument designed to be reused up to 10 times. Therefore, a method that ensures the complete elimination of microbial contaminants on the device is necessary. We studied the bactericidal efficacy of the Stella™ system, which purports to achieve this objective...
May 2017: Endoscopic Ultrasound
https://www.readbyqxmd.com/read/28616645/groundwater-contamination-with-2-6-dichlorobenzamide-bam-and-perspectives-for-its-microbial-removal
#5
REVIEW
Lea Ellegaard-Jensen, Benjamin Horemans, Bart Raes, Jens Aamand, Lars Hestbjerg Hansen
The pesticide metabolite 2,6-dichlorobenzamide (BAM) is very persistent in both soil and groundwater and has become one of the most frequently detected groundwater micropollutants. BAM is not removed by the physico-chemical treatment techniques currently used in drinking water treatment plants (DWTP); therefore, if concentrations exceed the legal threshold limit, it represents a sizeable problem for the stability and quality of drinking water production, especially in places that depend on groundwater for drinking water...
June 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28616498/xenotransplantation-a-special-case-of-one-health
#6
REVIEW
Joachim Denner
The chronic shortage of human transplants to treat tissue and organ failure has led to the development of xenotransplantation, the transplantation of cells, tissues and organs from another species to human recipients. For a number of reasons, pigs are best suited as donor animals. Successful, routine xenotransplantation would have an enormous impact on the health of the human population, including the young, who sometimes require a replacement organ or islet cells, but especially the elderly, who more often suffer the consequences of organ failure...
June 2017: One Health
https://www.readbyqxmd.com/read/28611499/lactobacillus-futsaii-cs3-a-new-gaba-producing-strain-isolated-from-thai-fermented-shrimp-kung-som
#7
Chatthaphisuth Sanchart, Onnicha Rattanaporn, Dietmar Haltrich, Pimpimol Phukpattaranont, Suppasil Maneerat
Kung-Som is a popular traditional Thai fermented shrimp product. It is rich in glutamic acid, which is the major substrate for the biosynthesis of gamma-aminobutyric acid (GABA) by lactic acid bacteria (LAB). In the present study, LAB from Kung-Som were isolated, screened for GABA formation, and the two isolates that transform glutamic acid most efficiently into GABA were identified. Based on the API-CHL50 fermentation profile and a phylogenetic tree of 16S rDNA sequences, strain CS3 and CS5 were identified as Lactobacillus futsaii, which was for the first time shown to be a promising GABA producer...
June 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28609746/biodegradation-of-hydrocarbon-mixtures-in-surface-waters-at-environmentally-relevant-levels-effect-of-inoculum-origin-on-kinetics-and-sequence-of-degradation
#8
Heidi Birch, Rikke Hammershøj, Mike Comber, Philipp Mayer
Biodegradation is a dominant removal process for many organic pollutants, and biodegradation tests serve as tools for assessing their environmental fate within regulatory risk assessment. In simulation tests, the inoculum is not standardized, varying in microbial quantity and quality, thereby potentially impacting the observed biodegradation kinetics. In this study we investigated the effect of inoculum origin on the biodegradation kinetics of hydrocarbons for five inocula from surface waters varying in urbanization and thus expected pre-exposure to petroleum hydrocarbons...
June 2, 2017: Chemosphere
https://www.readbyqxmd.com/read/28609116/the-underexposed-role-of-food-matrices-in-probiotic-products-reviewing-the-relationship-between-carrier-matrices-and-product-parameters
#9
Joost Flach, Mark van der Waal, Maurits van den Nieuwboer, Eric Claassen, Olaf Larsen
Probiotic microorganisms are increasingly incorporated into food matrices in order to confer proposed health benefits on the consumer. It is important that the health benefits, sensory properties, shelf-life and probiotic gastrointestinal tract (GIT) survival of these products are carefully balanced as they determine functionality and drive consumer acceptance. The strain-specific effects of probiotic species are imperative in this process but carrier matrices may play a pivotal role as well. This study therefore recapitulates the wealth of knowledge on carrier matrices and their interaction with probiotic strains...
June 13, 2017: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/28605941/application-and-possible-benefits-of-high-hydrostatic-pressure-or-high-pressure-homogenization-on-beer-processing-a-review
#10
Lígia Mr Santos, Fabiano A Oliveira, Elisa Hr Ferreira, Amauri Rosenthal
Beer is the most consumed beverage in the world, especially in countries such as USA, China and Brazil.It is an alcoholic beverage made from malted cereals, and the barley malt is the main ingredient, added with water, hops and yeast. High-pressure processing is a non-traditional method to preserve food and beverages. This technology has become more interesting compared to heat pasteurization, due to the minimal changes it brings to the original nutritional and sensory characteristics of the product, and it comprises two processes: high hydrostatic pressure, which is the most industrially used process, and high-pressure homogenization...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
https://www.readbyqxmd.com/read/28605726/the-exploration-of-monochromatic-near-infrared-led-improved-anoxygenic-photosynthetic-bacteria-rhodopseudomonas-sp-for-wastewater-treatment
#11
Xiang Qi, Yiwei Ren, Enling Tian, Xingzu Wang
The future wastewater treatment requires high-efficiency and energy-saving technology. Anoxygenic photosynthetic bacteria (APB) is deemed as an eco-friendly microorganism, which could be employed in wastewater treatment. Here, monochromatic near-infrared (MNIR) light emitting diode (LED) was used, and three key factors (light quality, light intensity and photoperiod) of it were analyzed by a response surface methodology (RSM) in APB wastewater treatment. The results showed that light quality was the biggest impact factor in APB wastewater treatment, and nearly 58...
June 3, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28604080/association-of-childhood-obesity-and-the-immune-system-a-systematic-review-of-reviews
#12
Roya Kelishadi, Mohammad Roufarshbaf, Sina Soheili, Farzaneh Payghambarzadeh, Mohsen Masjedi
The growing prevalence of childhood obesity has become a serious health problem over the past decades. As the immune system is greatly affected by excess weight, in this review of reviews, we discuss the findings of review articles about the relationship between childhood/maternal obesity and children's immune system. We searched English-language articles in PubMed, Scopus, ISI Thomson Reuters, and Google Scholar databases. All relevant reviews, either systematic or narrative, were retrieved. Then their quality was assessed by using the Assessment of Multiple Systematic Reviews and International Narrative Systematic Assessment tools, respectively...
June 12, 2017: Childhood Obesity
https://www.readbyqxmd.com/read/28601459/in-vitro-response-to-epa-dpa-and-dha-comparison-of-effects-on-ruminal-fermentation-and-biohydrogenation-of-18-carbon-fatty-acids-in-cows-and-ewes
#13
P G Toral, G Hervás, D Carreño, H Leskinen, A Belenguer, K J Shingfield, P Frutos
The modulation of milk fat nutritional quality through fish oil supplementation seems to be largely explained by the action of n-3 very long chain polyunsaturated fatty acids (PUFA) on ruminal biohydrogenation (BH) of C18 fatty acids (FA). However, relationships among this action, disappearance of those PUFA in the rumen, and potential detrimental consequences on ruminal fermentation remain uncertain. This study compared the effect of 20:5n-3 (eicosapentaenoic acid; EPA), 22:5n-3 (docosapentaenoic acid; DPA), and 22:6n-3 (docosahexaenoic acid; DHA) on rumen fermentation and BH of C18 FA and was conducted simultaneously in cows and sheep to provide novel insights into interspecies differences...
June 7, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28600313/novel-microbial-assemblages-dominate-weathered-sulfide-bearing-rock-from-copper-nickel-deposits-in-the-duluth-complex-minnesota-usa
#14
Daniel S Jones, Kim A Lapakko, Zachary J Wenz, Michael C Olson, Elizabeth W Roepke, Michael J Sadowsky, Paige J Novak, Jake V Bailey
The Duluth Complex in northeastern Minnesota hosts economically-significant deposits of copper, nickel, and platinum group elements (PGEs). The primary sulfide mineralogy of these deposits includes the minerals pyrrhotite, chalcopyrite, pentlandite, and cubanite, and weathering experiments show that most sulfide-bearing rock from the Duluth Complex generates moderately acidic leachate (pH 4-6). Microorganisms are important catalysts for metal sulfide oxidation and could influence water quality from mines in the Duluth Complex...
June 9, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28592834/impact-of-naturally-leaking-carbon-dioxide-on-soil-properties-and-ecosystems-in-the-qinghai-tibet-plateau
#15
Xiaohong Zhao, Hongzhang Deng, Wenke Wang, Feng Han, Chunrong Li, Hui Zhang, Zhenxue Dai
One of the major concerns for CO2 capture and storage (CCS) is the potential risk of CO2 leakage from storage reservoirs on the shallow soil property and vegetation. This study utilizes a naturally occurring CO2 leaking site in the Qinghai-Tibet Plateau to analog a "leaking CCS site". Our observations from this site indicates that long-term CO2 invasion in the vadose zone results in variations of soil properties, such as pH fluctuation, slight drop of total organic carbon, reduction of nitrogen and phosphorus, and concentration changes of soluble ions...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28592550/validation-and-implementation-of-clinical-laboratory-improvements-act-clia-compliant-whole-genome-sequencing-in-public-health-microbiology-laboratory
#16
Varvara K Kozyreva, Chau-Linda Truong, Alexander L Greninger, John Crandall, Rituparna Mukhopadhyay, Vishnu Chaturvedi
Public health microbiology laboratories (PHL) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Herein, we describe the validation of WGS on Illumina platform for routine use in PHL according to the Clinical Laboratory Improvements Act (CLIA) guidelines for the laboratory developed tests (LDTs). We developed a validation panel comprising ten Enterobacteriaceae isolates, five Gram-positive cocci, five Gram-negative non-fermenting species, nine Mycobacterium tuberculosis, and five miscellaneous bacteria...
June 7, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28584319/identification-and-characteristics-of-biological-agents-in-work-environment-of-medical-emergency-services-in-selected-ambulances
#17
Agata Bielawska-Drózd, Piotr Cieślik, Bożena Wlizło-Skowronek, Izabela Winnicka, Leszek Kubiak, Jolanta Jaroszuk-Ściseł, Daria Depczyńska, Justyna Bohacz, Teresa Korniłowicz-Kowalska, Ewa Skopińska-Różewska, Janusz Kocik
OBJECTIVES: Assessment of microbial air quality and surface contamination in ambulances and administration offices as a control place without occupational exposure to biological agents; based on quantitative and qualitative analysis of bacteria, yeasts and filamentous fungi found in collected samples. MATERIAL AND METHODS: The sampling was done by wet cyclone technology using the Coriolis recon apparatus, imprint and swab methods, respectively. In total, 280 samples from 28 ambulances and 10 offices in Warszawa were tested...
April 25, 2017: International Journal of Occupational Medicine and Environmental Health
https://www.readbyqxmd.com/read/28582498/microorganism-design-for-heterologous-biosynthesis-of-cannabinoids
#18
Carvalho Ângela, Esben Halkjær Hansen, Oliver Kayser, Simon Carlsen, Felix Stehle
During the last decade the use of medical Cannabis has expanded globally and legislation is getting more liberal in many countries, facilitating the research on cannabinoids. The unique interaction of cannabinoids with the human endocannabinoid system make these compounds an interesting target to be studied as a therapeutic agent for the treatment of several medical conditions. However, currently there are important limitations in the study, production and use of cannabinoids as pharmaceutical drugs. Besides the main constituent tetrahydrocannabinolic acid, the structurally related compound cannabidiol is of high interest as drug candidate...
June 4, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28579695/self-powered-autonomous-biological-oxygen-demand-biosensor-for-online-water-quality-monitoring
#19
Grzegorz Pasternak, John Greenman, Ioannis Ieropoulos
Standard Biological Oxygen Demand (BOD) analysis requires 5 days to complete. To date, microbial fuel cell biosensors used as an alternative method for BOD assessment requires external apparatus, which limits their use for on-line monitoring in remote, off-grid locations. In this study, a self-powered, floating biosensor was developed for online water quality monitoring. This approach eliminated the need for external apparatus and maintenance that would otherwise be required by other techniques. The biosensor was able to detect urine in freshwater and turn ON a visual and sound cues (85 dB)...
June 2017: Sensors and Actuators. B, Chemical
https://www.readbyqxmd.com/read/28578265/lactic-acid-bacteria-involved-in-cocoa-beans-fermentation-from-ivory-coast-species-diversity-and-citrate-lyase-production
#20
Hadja D Ouattara, Honoré G Ouattara, Michel Droux, Sylvie Reverchon, William Nasser, Sébastien L Niamke
Microbial fermentation is an indispensable process for high quality chocolate from cocoa bean raw material. lactic acid bacteria (LAB) are among the major microorganisms responsible for cocoa fermentation but their exact role remains to be elucidated. In this study, we analyzed the diversity of LAB in six cocoa producing regions of Ivory Coast. Ribosomal 16S gene sequence analysis showed that Lactobacillus plantarum and Leuconostoc mesenteroides are the dominant LAB species in these six regions. In addition, other species were identified as the minor microbial population, namely Lactobacillus curieae, Enterococcus faecium, Fructobacillus pseudoficulneus, Lactobacillus casei, Weissella paramesenteroides and Weissella cibaria...
May 17, 2017: International Journal of Food Microbiology
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