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Microorganism quality

J C Chandler, A Pérez-Méndez, J Paar, M M Doolittle, B Bisha, L D Goodridge
Fecal contamination of water poses a significant risk to public health due to the potential presence of pathogens, including enteric viruses. Therefore, sensitive, reliable and easy to use methods for the concentration, detection and quantification of microorganisms associated with the safety and quality of water are needed. In this study, we performed a field evaluation of an anion exchange resin based method to concentrate male-specific (F+) RNA coliphages (FRNA), fecal indicator organisms, from diverse environmental waters that were suspected to be contaminated with feces...
October 21, 2016: Journal of Virological Methods
Alicia Cervantes-Elizarrarás, Javier Piloni-Martini, Esther Ramírez-Moreno, Ernesto Alanís-García, Norma Güemes-Vera, Carlos Alberto Gómez-Aldapa, Quinatzin Yadira Zafra-Rojas, Nelly Del Socorro Cruz-Cansino
The purpose of this research was to optimize the thermoultrasound conditions for blackberry juice using the response surface methodology and considering juice quality parameters and antioxidant capacity. With the exception of microbial growth, the response variables showed high correlation coefficients with the mathematical model (R(2)adj>0.91). Thermoultrasound treatment inactivated all the evaluated microorganisms, and at the optimum conditions (50±1°C at 17±1min) it increased enzyme inactivation and antioxidant activity in comparison to pasteurized juice...
January 2017: Ultrasonics Sonochemistry
Abidelfatah M Nasser, Neta-Lee Benisti, Naomi Ofer, Sivan Hovers, Yeshayahu Nitzan
Advanced wastewater treatment processes are applied to prevent the environmental dissemination of pathogenic microorganisms. Giardia lamblia causes a severe disease called giardiasis, and is highly prevalent in untreated wastewater worldwide. Monitoring the microbial quality of wastewater effluents is usually based on testing for the levels of indicator microorganisms in the effluents. This study was conducted to compare the suitability of fecal coliforms, F+ coliphages and sulfide reducing clostridia (SRC) as indicators for the reduction of Giardia cysts in two full-scale wastewater treatment plants...
October 21, 2016: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Mahboob Nemati, Aliasghar Hamidi, Solmaz Maleki Dizaj, Vahid Javaherzadeh, Farzaneh Lotfipour
Traditional microbiological methods tend to be labor-intensive and time-consuming. Rapid and novel methods in microbiological tests provide more sensitive, precise and reproducible results compared with conventional methods. In microbiology, the most rapid testing methods belong to the field of biotechnology such as PCR, ELISA, ATP bioluminescence and etc. Nevertheless impedance microbiology, biosensors and analytical procedures to determine microbial constituents are of significance. The present review article was conducted using internet databases and related scientific literatures and articles that provide information on developments in the rapid methods in microbiology...
September 2016: Advanced Pharmaceutical Bulletin
Jingqing Liu, Hongxing Ren, Xianbei Ye, Wei Wang, Yan Liu, Liping Lou, Dongqing Cheng, Xiaofang He, Xiaoyan Zhou, Shangde Qiu, Liusong Fu, Baolan Hu
Biofilms in the pipe wall may lead to water quality deterioration and biological instability in drinking water distribution systems (DWDSs). In this study, bacterial community radial-spatial distribution in biofilms along the pipe wall in a chlorinated DWDS of East China was investigated. Three pipes of large diameter (300, 600, and 600 mm) were sampled in this DWDS, including a ductile cast iron pipe (DCIP) with pipe age of 11 years and two gray cast iron pipes (GCIP) with pipe ages of 17 and 19 years, and biofilms in the upper, middle, and lower parts of each pipe wall were collected...
October 19, 2016: Applied Microbiology and Biotechnology
Katrin Ochsenreither, Claudia Glück, Timo Stressler, Lutz Fischer, Christoph Syldatk
Polyunsaturated fatty acids (PUFAs) of the ω-3 and ω-6 class (e.g., α-linolenic acid, linoleic acid) are essential for maintaining biofunctions in mammalians like humans. Due to the fact that humans cannot synthesize these essential fatty acids, they must be taken up from different food sources. Classical sources for these fatty acids are porcine liver and fish oil. However, microbial lipids or single cell oils, produced by oleaginous microorganisms such as algae, fungi and bacteria, are a promising source as well...
2016: Frontiers in Microbiology
Taous Saraoui, Josiane Cornet, Emilie Guillouet, Marie France Pilet, Frédérique Chevalier, Jean-Jacques Joffraud, Françoise Leroi
Tropical shrimp is of considerable economic importance in the world but is highly perishable due to microbial and chemical degradation. Biopreservation is a food preservation technology based on the addition of "positive" bacteria able to kill or prevent the growth of undesirable microorganisms. Two strains of lactic acid bacteria (LAB) have previously been selected for a biopreservation strategy: Lactococcus piscium CNCM I-4031, for its ability to prevent the sensory deterioration of seafood and Carnobacterium divergens V41, which inhibits growth of Listeria monocytogenes...
October 3, 2016: International Journal of Food Microbiology
Giuseppe Dionisio, Per Kryger, Tove Steenberg
Beauveria bassiana is an entomopathogenic fungus that grows both in vivo and in vitro. In vivo it can colonize live insect hosts, and tissue digestion occurs by secreted hydrolytic exoenzymes. It can also colonize dead insect tissue provided this is free from competing microorganisms. Depending on whether the host is alive or dead the expression (quality/quantity) of the exoenzymes may vary. We have grown several isolates of B. bassiana in shaking flasks for 120 h at 25 °C in order to evaluate the maximal exoenzyme production using two diet regimes...
October 14, 2016: Insects
Maria Schirone, Pierina Visciano, Rosanna Tofalo, Giovanna Suzzi
The consumption of food containing high amounts of histamine and other biogenic amines can cause food poisoning with different symptoms linked to the individual sensitivity and the detoxification activity. Histamine is the only biogenic amine with regulatory limits set by the European Commission in fish and fishery products, because it can lead to a fatal outcome. However, also fermented foods can be involved in outbreaks and sporadic cases of intoxication. The factors affecting the presence of histamine in food are variable and product specific including the availability of the precursor amino acid, the presence of microorganisms producing decarboxylases, and the conditions allowing their growth and enzyme production...
October 18, 2016: Handbook of Experimental Pharmacology
Waroon Khota, Suradej Pholsen, David Higgs, Yimin Cai
Natural lactic acid bacteria (LAB) populations in tropical grasses and their fermentation characteristics on silage prepared with cellulase enzyme and LAB inoculants were studied. A commercial inoculant Lactobacillus plantarum Chikuso 1 (CH), a local selected strain Lactobacillus casei TH14 (TH14), and 2 cellulases, Acremonium cellulase (AC) and Maicelase (MC; Meiji Seika Pharma Co. Ltd., Tokyo, Japan), were used as additives to silage preparation with fresh and wilted (6 h) Guinea grass and Napier grass. Silage was prepared using a laboratory-scale fermentation system...
October 13, 2016: Journal of Dairy Science
Denes Kaic Alves do Rosário, Yhan da Silva Mutz, Jaqueline Moreira Curtis Peixoto, Syllas Borburema Silva Oliveira, Raquel Vieira de Carvalho, Joel Camilo Souza Carneiro, Jackline Freitas Brilhante de São José, Patrícia Campos Bernardes
New sanitization methods have been evaluated to improve food safety and food quality and to replace chlorine compounds. However, these new methods can lead to physicochemical and sensory changes in fruits and vegetables. The present study evaluated the effects of acetic acid, peracetic acid, and sodium dodecylbenzenesulfonate isolated or combined with 5min of ultrasound treatment (40kHz, 500W) on strawberry quality over 9days of storage at 8°C. The strawberry natural contaminant microbiota (molds and yeasts, mesophilic aerobic and lactic acid bacteria), physicochemical quality (pH, total titratable acidity, total soluble solids, vitamin C, and color), sensory quality (triangle test) and inactivation of Salmonella enterica subsp...
October 12, 2016: International Journal of Food Microbiology
Jutta Speda, Mikaela A Johansson, Uno Carlsson, Martin Karlsson
Enzyme discovery in individual strains of microorganisms is compromised by the limitations of pure culturing. In principle, metaproteomics allows for fractionation and study of different parts of the protein complement but has hitherto mainly been used to identify intracellular proteins. However, the extracellular environment is also expected to comprise a wealth of information regarding important proteins. An absolute requirement for metaproteomic studies of protein expression, and irrespective of downstream methods for analysis, is that sample preparation methods provide clean, concentrated and representative samples of the protein complement...
October 11, 2016: Analytical Biochemistry
K J Hornig, B A Burgess, N T Saklou, V Johnson, A Malmlov, D C Van Metre, P S Morley, S R Byers
BACKGROUND: Infection control is critical to providing high-quality patient care. Many veterinary teaching hospitals (VTHs) utilize footbaths or footmats at entrances and key control points throughout the facility to decrease trafficking of pathogenic microorganism on contaminated footwear. HYPOTHESIS/OBJECTIVES: To compare efficacy of 4 disinfectants used in footmats for decreasing bacterial contamination of footwear in a large animal hospital. ANIMALS: A single adult dairy cow was housed in a stall for 4 days to facilitate stall contamination with fecal material...
October 12, 2016: Journal of Veterinary Internal Medicine
Jie Fu, Jingqiu Wang, Yanping Luo, Lifeng Zhang, Yuan Zhang, Xinfang Dong, Hongjuan Yu, Mingqiang Cao, Xingming Ma
BACKGROUND: The role of MASP-2 is vital in the process of complement activation by the lectin pathway. It is generally considered that the functional activation of MASP-2 contribute to the infection disease development process. AIMS: To analyze the association between MASP-2 functional gene (rs72550870) polymorphism and the infection disease risk by a meta-analysis. METHOD: Relevant case-control studies were identified by searching Cochrane Library, PubMed, Emabase, DOAJ, CAB Abstracts, CSA, CINAHL, EBSCO, Scopus, Global Health, Index Copernicus, CA, China National Knowledge Infrastructure (CNKI) databases up to 10th January 2016...
October 7, 2016: Microbial Pathogenesis
Daniele Alberoni, Francesca Gaggìa, Loredana Baffoni, Diana Di Gioia
Nowadays, honey bees are stressed by a number of biotic and abiotic factors which may compromise to some extent the pollination service and the hive productivity. The EU ban of antibiotics as therapeutic agents against bee pathogens has stimulated the search for natural alternatives. The increasing knowledge on the composition and functions of the bee gut microbiota and the link between a balanced gut microbiota and health status have encouraged the research on the use of gut microorganisms to improve bee health...
October 8, 2016: Applied Microbiology and Biotechnology
Igor E Agranovski, Evgeny V Usachev, Elina Agranovski, Olga V Usacheva
AIMS: A portable bioaerosol monitor is highly demanded technology in many areas including air quality control, occupational exposure assessment and health risk evaluation, environmental studies and, especially, in defence and bio-terrorism applications. Our recent groundwork allowed us to formulate the concept of a portable bioaerosol monitor, which ought to be light, user friendly, reliable and capable of detecting airborne pathogens within 1 -1.5 hours at the spot. METHODS AND RESULTS: Conceptually, the event of bioaerosol concentration burst is determined by triggers, commencing representative air sampling with sequential real-time PCR confirmation of the targeted microorganism presence in the air...
October 6, 2016: Journal of Applied Microbiology
Jie Fu, Wan-Ning Lee, Clark Coleman, Melissa Meyer, Jason Carter, Kirk Nowack, Ching-Hua Huang
A pilot study employing two parallel trains of two-stage biofiltration, i.e., a sand/anthracite (SA) biofilter followed by a biologically-active granular activated carbon (GAC) contactor, was conducted to test the efficiency, feasibility and stability of biofiltration for removing natural organic matter (NOM) after coagulation in a drinking water treatment plant. Results showed the biofiltration process could effectively remove turbidity (<0.1 NTU in all effluents) and NOM (>24% of dissolved organic carbon (DOC), >57% of UV254, and >44% of SUVA254), where the SA biofilters showed a strong capacity for turbidity removal, while the GAC contactors played the dominant role in NOM removal...
January 2017: Chemosphere
Kevin C Sales, Filipa Rosa, Bernardo R Cunha, Pedro N Sampaio, Marta B Lopes, Cecília R C Calado
Escherichia coli is one of the most used host microorganism for the production of recombinant products, such as heterologous proteins and plasmids. However genetic, physiological and environmental factors influence the plasmid replication and cloned gene expression in a highly complex way. To control and optimize the recombinant expression system performance, it is very important to understand this complexity. Therefore, the development of rapid, highly sensitive and economic analytical methodologies, which enable the simultaneous characterization of heterologous product synthesis and physiologic cell behavior under a variety of culture conditions, is highly desirable...
October 3, 2016: Biotechnology Progress
Jairo Amaya-Guio, David Andres Viveros-Carreño, Eloisa Mercedes Sierra-Barrios, Mercy Yolima Martinez-Velasquez, Carlos F Grillo-Ardila
BACKGROUND: Bacterial vaginosis (BV) is an infection that has a prevalence between 10% to 50% worlwide. BV results in an imbalance of the normal vaginal flora. Microorganisms associated with BV have been isolated from the normal flora of the male genital tract, and their presence could be related to the recurrence of BV after antibiotic treatment. Therefore, the treatment of sexual partners could decrease the recurrence of infection and possibly the burden of the disease. OBJECTIVES: To assess the effectiveness in women and the safety in men of concurrent antibiotic treatment for the sexual partners of women treated for BV...
October 1, 2016: Cochrane Database of Systematic Reviews
Zackary W Munger, Cayelan C Carey, Alexandra B Gerling, Kathleen D Hamre, Jonathan P Doubek, Spencer D Klepatzki, Ryan P McClure, Madeline E Schreiber
The accumulation of Fe and Mn in seasonally stratified drinking water reservoirs adversely impacts water quality. To control issues with Fe and Mn at the source, some drinking water utilities have deployed hypolimnetic oxygenation systems to create well-oxygenated conditions in the water column that are favorable for the oxidation, and thus removal, of Fe and Mn. However, in addition to being controlled by dissolved oxygen (DO), Fe and Mn concentrations are also influenced by pH and metal-oxidizing microorganisms...
September 20, 2016: Water Research
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