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Riboflavin molecular

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https://www.readbyqxmd.com/read/27830356/successful-treatment-of-a-patient-with-ethylmalonic-encephalopathy-by-intravenous-n-acetylcysteine
#1
Mustafa Kılıç, Özge Dedeoğlu, Rahşan Göçmen, Selman Kesici, Deniz Yüksel
Ethylmalonic encephalopathy (EE) is an autosomal recessive devastating metabolic disorder affecting the brain, gastrointestinal tract, peripheral vessels and rarely the other vascular organs. We report a 10-month-old girl who presented as a meningococcemia clinic but later diagnosed ethylmalonic encephalopathy. Molecular analyses revealed a homozygous c.554 T > G; p. L185R mutation in ETHE1 gene. She was only partially benefited from riboflavine, coenzyme Q10, metronidazole, N-acetylcysteine and symptomatic treatment and discharged from hospital with the sequela of oxygene dependance and developmental delay...
November 9, 2016: Metabolic Brain Disease
https://www.readbyqxmd.com/read/27791453/new-biotechnological-applications-for-ashbya-gossypii-challenges-and-perspectives
#2
Tatiana Q Aguiar, Rui Silva, Lucília Domingues
The filamentous fungus Ashbya gossypii has long been considered a paradigm of the White Biotechnology in what concerns riboflavin production. Its industrial relevance led to the development of a significant molecular and in silico modeling toolbox for its manipulation. This, together with the increasing knowledge of its genome and metabolism has helped designing effective metabolic engineering strategies for optimizing riboflavin production, but also for developing new A. gossypii strains for novel biotechnological applications, such as production of recombinant proteins, single cell oils (SCOs), and flavour compounds...
October 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27790197/the-nitrogen-availability-interferes-with-mycorrhiza-induced-resistance-against-botrytis-cinerea-in-tomato
#3
Paloma Sanchez-Bel, Pilar Troncho, Jordi Gamir, Maria J Pozo, Gemma Camañes, Miguel Cerezo, Víctor Flors
Mycorrhizal plants are generally quite efficient in coping with environmental challenges. It has been shown that the symbiosis with arbuscular mycorrhizal fungi (AMF) can confer resistance against root and foliar pathogens, although the molecular mechanisms underlying such mycorrhiza-induced resistance (MIR) are poorly understood. Tomato plants colonized with the AMF Rhizophagus irregularis display enhanced resistance against the necrotrophic foliar pathogen Botrytis cinerea. Leaves from arbuscular mycorrhizal (AM) plants develop smaller necrotic lesions, mirrored also by a reduced levels of fungal biomass...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27757515/high-throughput-and-simultaneous-quantitative-analysis-of-homocysteine-methionine-cycle-metabolites-and-co-factors-in-blood-plasma-and-cerebrospinal-fluid-by-isotope-dilution-lc-ms-ms
#4
Seu Ping Guiraud, Ivan Montoliu, Laeticia Da Silva, Loïc Dayon, Antonio Núñez Galindo, John Corthésy, Martin Kussmann, Francois-Pierre Martin
The methionine cycle is a key pathway contributing to the regulation of human health, with well-established involvement in cardiovascular diseases and cognitive function. Changes in one-carbon cycle metabolites have also been associated with mild cognitive decline, vascular dementia, and Alzheimer's disease. Today, there is no single analytical method to monitor both metabolites and co-factors of the methionine cycle. To address this limitation, we here report for the first time a new method for the simultaneous quantitation of 17 metabolites in the methionine cycle, which are homocysteic acid, taurine, serine, cysteine, glycine, homocysteine, riboflavin, methionine, pyridoxine, cystathionine, pyridoxamine, S-adenosylhomocysteine, S-adenosylmethionine, betaine, choline, dimethylglycine, and 5-methyltetrahydrofolic acid...
October 18, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27709903/in-situ-molecular-imaging-of-the-biofilm-and-its-matrix
#5
Yuanzhao Ding, Yufan Zhou, Juan Yao, Craig Szymanski, James K Fredrickson, Liang Shi, Bin Cao, Zihua Zhu, Xiao-Ying Yu
Molecular mapping of live biofilms at submicron resolution presents a grand challenge. Here, we present the first chemical mapping results of biofilm extracellular polymeric substance (EPS) components in biofilms using correlative imaging be-tween super resolution florescence microscopy and liquid time-of-flight secondary ion mass spectrometry (ToF-SIMS). She-wanella oneidensis is used as a model organism. Heavy metal anions chromate (Cr2O72-) consisting of chromium Cr (VI) was a model environmental stressor used to treat the biofilms...
October 6, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27620349/vitamin-b2-as-a-virulence-factor-in-pseudogymnoascus-destructans-skin-infection
#6
Miroslav Flieger, Hana Bandouchova, Jan Cerny, Milada Chudíčková, Miroslav Kolarik, Veronika Kovacova, Natália Martínková, Petr Novák, Ondřej Šebesta, Eva Stodůlková, Jiri Pikula
Pathogenic and non-pathogenic related microorganisms differ in secondary metabolite production. Here we show that riboflavin overproduction by a fungal pathogen and its hyperaccumulation in affected host tissue exacerbates a skin infection to necrosis. In white-nose syndrome (WNS) skin lesions caused by Pseudogymnoascus destructans, maximum riboflavin concentrations reached up to 815 μg ml(-1), indicating bioaccumulation and lack of excretion. We found that high riboflavin concentrations are cytotoxic under conditions specific for hibernation, affect bats' primary fibroblasts and induce cell detachment, loss of mitochondrial membrane potential, polymerization of cortical actin, and cell necrosis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27605716/alkaline-stress-and-iron-deficiency-regulate-iron-uptake-and-riboflavin-synthesis-gene-expression-differently-in-root-and-leaf-tissue-implications-for-iron-deficiency-chlorosis
#7
En-Jung Hsieh, Brian M Waters
Iron (Fe) is an essential mineral that has low solubility in alkaline soils, where its deficiency results in chlorosis. Whether low Fe supply and alkaline pH stress are equivalent is unclear, as they have not been treated as separate variables in molecular physiological studies. Additionally, molecular responses to these stresses have not been studied in leaf and root tissues simultaneously. We tested how plants with the Strategy I Fe uptake system respond to Fe deficiency at mildly acidic and alkaline pH by measuring root ferric chelate reductase (FCR) activity and expression of selected Fe uptake genes and riboflavin synthesis genes...
October 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27591616/surface-enhanced-raman-spectroscopy-sers-in-food-analytics-detection-of-vitamins-b2-and-b12-in-cereals
#8
Andreea Ioana Radu, Maria Kuellmer, Bernd Giese, Uwe Huebner, Karina Weber, Dana Cialla-May, Jürgen Popp
Food analysis has been gaining interest throughout recent decades for different reasons: the detection of hazardous substances in food and routine investigations of food composition and vitamin/nutrient contents. Regardless of the targeted component, food analysis raises a few challenges regarding the complexity of the matrix and detecting trace amounts of substances. We report herein the results obtained regarding the simultaneous detection of two B vitamins (riboflavin, vitamin B2 and cyanocobalamin, vitamin B12) by means of SERS...
November 1, 2016: Talanta
https://www.readbyqxmd.com/read/27576102/hyaluronan-cholesterol-nanohydrogels-characterisation-and-effectiveness-in-carrying-alginate-lyase-enzyme
#9
Elita Montanari, Chiara Di Meo, Simona Sennato, Antonio Francioso, Anna Laura Marinelli, Francesca Ranzo, Serena Schippa, Tommasina Coviello, Federico Bordi, Pietro Matricardi
Although in recent years several methods have been studied and developed to obtain different types of nanosized drug delivery systems, the set up of suitable procedures and materials remains highly expensive, their preparation is time consuming and often not feasible for a scale-up process. Furthermore, the sterilisation and storage of nanocarrier formulations represents a complicated but mandatory step for their effective use. In our previous work we assessed the use of an autoclaving process to achieve, in one simple step, sterile self-assembled hyaluronan-cholesterol (HA-CH) and hyaluronan-riboflavin (HA-Rfv) nanohydrogels (NHs)...
August 26, 2016: New Biotechnology
https://www.readbyqxmd.com/read/27550932/gene-bb0318-is-critical-for-the-oxidative-stress-response-and-infectivity-of-borrelia-burgdorferi
#10
Adrienne C Showman, George Aranjuez, Philip P Adams, Mollie W Jewett
A greater understanding of the molecular mechanisms that Borrelia burgdorferi uses to survive during mammalian infection is critical for the development of novel diagnostic and therapeutic tools to improve the clinical management of Lyme disease. Using an In Vivo Expression Technology (IVET)-based approach to identify B. burgdorferi in vivo expressed genes, we discovered gene bb0318, which is thought to encode the ATPase component of a putative riboflavin ABC transport system. Riboflavin is a critical metabolite for all organisms to maintain redox homeostasis...
August 22, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27490826/identification-and-characterization-of-the-missing-phosphatase-on-the-riboflavin-biosynthesis-pathway-in-arabidopsis-thaliana
#11
Na Sa, Renu Rawat, Chelsea Thornburg, Kevin D Walker, Sanja Roje
Despite the importance of riboflavin as the direct precursor of the cofactors flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN), the physiologically relevant catalyst dephosphorylating the riboflavin biosynthesis pathway intermediate 5-amino-6-ribitylamino-2,4(1H,3H) pyrimidinedione 5'-phosphate (ARPP) has not been characterized from any organism. By using as the query sequence a previously identified plastidial FMN hydrolase AtcpFHy1 (At1g79790), belonging to the haloacid dehalogenase (HAD) superfamily, seven candidates for the missing ARPP phosphatase were found, cloned, recombinantly expressed, and purified...
August 4, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/27479840/effect-of-cu-2-complexation-on-the-scavenging-ability-of-chrysin-towards-photogenerated-singlet-molecular-oxygen-o2-1-%C3%AE-g-possible-biological-implications
#12
Vanesa A Muñoz, Gabriela V Ferrari, M Paulina Montaña, Sandra Miskoski, Norman A García
Visible-light irradiation of aqueous-ethanolic solutions of Riboflavin (Rf) in the individual presence of the flavone chrysin (Chr) and its complex with Cu(2+) ([Chr2Cu]; 2:1 L:M) generates singlet molecular oxygen O2((1)Δg), that concomitantly interact with both flavone derivatives. Overall (kt) and reactive (kr) rate constants in the order of 10(7)M(-1)s(-1) were determined for the process. Metal chelation greatly enhances the scavenging ability of [Chr2Cu] towards O2((1)Δg) through a mechanism dominated, in >80%, by the physical component...
September 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27316765/preventive-role-of-lens-antioxidant-defense-mechanism-against-riboflavin-mediated-sunlight-damaging-of-lens-crystallins
#13
Afrooz Anbaraki, Kazem Khoshaman, Younes Ghasemi, Reza Yousefi
The main components of sunlight reaching the eye lens are UVA and visible light exerting their photo-damaging effects indirectly by the aid of endogenous photosensitizer molecules such as riboflavin (RF). In this study, lens proteins solutions were incubated with RF and exposed to the sunlight. Then, gel mobility shift analysis and different spectroscopic assessments were applied to examine the structural damaging effects of solar radiation on these proteins. Exposure of lens proteins to direct sunlight, in the presence of RF, leads to marked structural crosslinking, oligomerization and proteolytic instability...
October 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27315123/vitamins-for-enhancing-plant-resistance
#14
REVIEW
Hatem Boubakri, Mahmoud Gargouri, Ahmed Mliki, Faiçal Brini, Julie Chong, Moez Jbara
This paper provides an overview on vitamins with inducing activities in plants, the molecular and cellular mechanisms implicated, and the hormonal signalling-network regulating this process. Moreover, it reports how vitamins might be part of the molecular events linked to induced resistance by the conventional elicitors. Induced resistance (IR), exploiting the plant innate-defense system is a sustainable strategy for plant disease control. In the last decade, vitamins have been proven to act as inducers of disease resistance, and these findings have received an important attention owing to their safety and cost effectiveness...
September 2016: Planta
https://www.readbyqxmd.com/read/27271694/riboflavin-transport-and-metabolism-in-humans
#15
Maria Barile, Teresa Anna Giancaspero, Piero Leone, Michele Galluccio, Cesare Indiveri
Recent studies elucidated how riboflavin transporters and FAD forming enzymes work in humans and create a coordinated flavin network ensuring the maintenance of cellular flavoproteome. Alteration of this network may be causative of severe metabolic disorders such as multiple acyl-CoA dehydrogenase deficiency (MADD) or Brown-Vialetto-van Laere syndrome. A crucial step in the maintenance of FAD homeostasis is riboflavin uptake by plasma and mitochondrial membranes. Therefore, studies on recently identified human plasma membrane riboflavin transporters are presented, together with those in which still unidentified mitochondrial riboflavin transporter(s) have been described...
July 2016: Journal of Inherited Metabolic Disease
https://www.readbyqxmd.com/read/27266856/bound-flavin-cytochrome-model-of-extracellular-electron-transfer-in-shewanella-oneidensis-analysis-by-free-energy-molecular-dynamics-simulations
#16
Gongyi Hong, Ruth Pachter
Flavins are known to enhance extracellular electron transfer (EET) in Shewanella oneidensis MR-1 bacteria, which reduce electron acceptors through outer-membrane (OM) cytochromes c. Free-shuttle and bound-redox cofactor mechanisms were proposed to explain this enhancement, but recent electrochemical reports favor a flavin-bound model, proposing two one-electron reductions of flavin, namely, oxidized (Ox) to semiquinone (Sq) and semiquinone to hydroquinone (Hq), at anodic and cathodic conditions, respectively...
June 30, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27083835/physico-chemical-properties-of-alginate-shellac-aqueous-core-capsules-influence-of-membrane-architecture-on-riboflavin-release
#17
Ghazi Ben Messaoud, Laura Sánchez-González, Laurent Probst, Carole Jeandel, Elmira Arab-Tehrany, Stéphane Desobry
To enhance physico-chemical properties of alginate liquid-core capsules, shellac was incorporated into the membrane (composite capsules) or as an additional external layer (coated capsules). The influence of pH, coating time, shellac concentration and preparation mechanism (acid or calcium precipitation) were investigated. Results showed that shellac significantly influenced the capsules properties. The feasibility of shellac incorporation was closely related to the preparation conditions as confirmed by Infrared spectroscopy...
June 25, 2016: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27082285/vancomycin-sensitized-photooxidation-in-the-presence-of-the-natural-pigment-vitamin-b2-interaction-with-excited-states-and-photogenerated-ros
#18
D Cacciari, E Reynoso, M B Spesia, S Criado, M A Biasutti
: Sensitized photooxidation processes in the presence of natural pigments may provide an alternative to antibiotics degradation since these compounds are transparent to natural light irradiation, therefore, they can be degraded by the action of photosensitizers which absorb light and produce highly reactive species, especially those derived from molecular oxygen (ROS). Most antibiotics used currently belong to a group of pharmaceutical substances that have been considered a new type of contaminants due to their persistence and bioaccumulation in the environment...
April 15, 2016: Redox Report: Communications in Free Radical Research
https://www.readbyqxmd.com/read/27054585/riboflavin-induced-disease-resistance-requires-the-mitogen-activated-protein-kinases-3-and-6-in-arabidopsis-thaliana
#19
Shengjun Nie, Huilian Xu
As a resistance elicitor, riboflavin (vitamin B2) protects plants against a wide range of pathogens. At molecular biological levels, it is important to elucidate the signaling pathways underlying the disease resistance induced by riboflavin. Here, riboflavin was tested to induce resistance against virulent Pseudomonas syringae pv. Tomato DC3000 (Pst DC3000) in Arabidopsis. Results showed that riboflavin induced disease resistance based on MAPK-dependent priming for the expression of PR1 gene. Riboflavin induced transient expression of PR1 gene...
2016: PloS One
https://www.readbyqxmd.com/read/27021064/identification-of-possible-light-emitters-in-the-gills-of-a-bioluminescent-fungus-mycena-chlorophos
#20
Katsunori Teranishi
The pileus of Mycena chlorophos actively, spontaneously, and continuously emits green light. Molecular mechanisms underlying this bioluminescence remain unclear. We investigated light emitters in the pileus of M. chlorophos to determine the underlying mechanisms. High-performance liquid chromatography-fluorescence-photodiode array-mass detection analyses showed that actively luminescent gills in the pileus exclusively and abundantly possessed riboflavin, riboflavin 5'-monophosphate, and flavin adenine dinucleotide as green-fluorescent components...
March 28, 2016: Luminescence: the Journal of Biological and Chemical Luminescence
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