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Riboflavin molecular

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https://www.readbyqxmd.com/read/28411331/glutaric-acidemia-type-1-a-case-of-infantile-stroke
#1
Gül Demet Kaya Ozcora, Songul Gokay, Mehmet Canpolat, Fatih Kardaş, Mustafa Kendirci, Sefer Kumandaş
BACKGROUND: Glutaric acidemia Type 1 (GA-1) is an autosomal recessively inherited metabolic disorder which is associated with GCDH gene mutations which alters the glutaryl-CoA dehydrogenase, an enzyme playing role in the catabolic pathways of the amino acids lysine, hydroxylysine, and tryptophan. Clinical findings are often encephalopathic crises, dystonia, and extrapyramidal symptoms. CASE REPORT: A 9-month-old male infant referred to our department with focal tonic-clonic seizures during rotavirus infection and acute infarcts in MRI...
April 15, 2017: JIMD Reports
https://www.readbyqxmd.com/read/28407967/molecular-characterization-of-an-unauthorized-genetically-modified-bacillus-subtilis-production-strain-identified-in-a-vitamin-b2-feed-additive
#2
Valentina Paracchini, Mauro Petrillo, Ralf Reiting, Alexandre Angers-Loustau, Daniela Wahler, Andrea Stolz, Birgit Schönig, Anastasia Matthies, Joachim Bendiek, Dominik M Meinel, Sven Pecoraro, Ulrich Busch, Alex Patak, Joachim Kreysa, Lutz Grohmann
Many food and feed additives result from fermentation of genetically modified (GM) microorganisms. For vitamin B2 (riboflavin), GM Bacillus subtilis production strains have been developed and are often used. The presence of neither the GM strain nor its recombinant DNA is allowed for fermentation products placed on the EU market as food or feed additive. A vitamin B2 product (80% feed grade) imported from China was analysed. Viable B. subtilis cells were identified and DNAs of two bacterial isolates (LHL and LGL) were subjected to three whole genome sequencing (WGS) runs with different devices (MiSeq, 454 or HiSeq system)...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28394446/laser-ablation-electrospray-ionization-mass-spectrometry-with-ion-mobility-separation-reveals-metabolites-in-the-symbiotic-interactions-of-soybean-roots-and-rhizobia
#3
Sylwia A Stopka, Beverly J Agtuca, David W Koppenaal, Ljiljana Paša-Tolić, Gary Stacey, Akos Vertes, Christopher R Anderton
Technologies enabling in situ metabolic profiling of living plant systems are invaluable for understanding physiological processes and could be used for rapid phenotypic screening (e.g., to produce plants with superior biological nitrogen fixing ability). The symbiotic interaction between legumes and nitrogen-fixing soil bacteria results in a specialized plant organ (i.e., root nodule) where the exchange of nutrients between host and endosymbiont occurs. Laser ablation electrospray ionization mass spectrometry (LAESI-MS) is a method that can be performed under ambient conditions requiring minimal sample preparation...
April 10, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28367977/riboflavin-deficiency-induces-a-significant-change-in-proteomic-profiles-in-hepg2-cells
#4
Zhonghao Xin, Lingling Pu, Weina Gao, Yawen Wang, Jingyu Wei, Tala Shi, Zhanxin Yao, Changjiang Guo
Riboflavin deficiency is widespread in many regions over the world, especially in underdeveloped countries. In this study, we investigated the effects of riboflavin deficiency on protein expression profiles in HepG2 cells in order to provide molecular information for the abnormalities induced by riboflavin deficiency. HepG2 cells were cultured in media containing different concentrations of riboflavin. Changes of cell viability and apoptosis were assessed. A comparative proteomic analysis was performed using a label-free shotgun method with LC-MS/MS to investigate the global changes of proteomic profiles in response to riboflavin deficiency...
April 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28330106/enhanced-production-of-pectinase-by-aspergillus-terreus-ncft-4269-10-using-banana-peels-as-substrate
#5
Bijay Kumar Sethi, Prativa Kumari Nanda, Santilata Sahoo
Aspergillus terreus NCFT4269.10 was implemented in solid-state (SSF) and liquid static surface fermentation (LSSF) for biosynthesis of pectinase. Amongst various substrates, like, mustard oil cake, neem oil cake, groundnut oil cake, black gram peels, green gram peels, chickling vetch peels/grass pea peels wheat bran, pearl millet residues, finger millet waste, broken rice, banana peels (BP), apple pomace (AP) and orange peels, banana peel (Musa paradisiaca L.; Family: Musaceae) was most suitable for pectinase biosynthesis (LSSF: 400 ± 21...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28325140/vitamins-in-heart-failure-friend-or-enemy
#6
George Georgiopoulos, Christina Chrysohoou, Georgia Vogiatzi, Nikolaos Magkas, Ippokratis Bournelis, Sofia Bampali, Damien Gruson, Dimitris Tousoulis
BACKGROUND: The failing heart is characterized by a depleted metabolic energy reserve and the up-regulation of several molecular mechanisms leading to cardiac hypertrophy, inflammation, fibrosis, angiogenesis, and apoptosis. Dietary or non-dietary supplementation of vitamins could potentially benefit energy balance. OBJECTIVE: The objective of the present study was to evaluate all available information on vitamins supplementation in patients with chronic HF for possible beneficial effect on metabolic, inotropic, chronotropic and hemodynamic indices...
March 20, 2017: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/28276274/multi-spectroscopic-and-molecular-modelling-approach-to-investigate-the-interaction-of-riboflavin-with-human-serum-albumin
#7
Md Maroof Alam, Faizan Abul Qais, Iqbal Ahmad, Parvez Alam, Rizwan Hasan Khan, Imrana Naseem
Riboflavin (RF) plays an important role in various metabolic redox reactions in the form of flavin adenine dinucleotide and flavin mononucleotide. Human serum albumin (HSA) is an important protein involved in the transportation of drugs, hormones, fatty acid and other molecules which determine the biodistribution and physiological fate of these molecules. In this study, we have investigated the interaction of riboflavin RF with HSA under simulative physiological conditions using various biophysical, calorimetric and molecular docking techniques...
March 9, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28149672/corneal-cross-linking-with-riboflavin-and-uv-a-in-the-mouse-cornea-in-vivo-morphological-biochemical-and-physiological-analysis
#8
Sabine Kling, Arthur Hammer, Alain Conti, Farhad Hafezi
PURPOSE: To morphologically, biochemically, and physiologically characterize corneal cross-linking with riboflavin and UV-A light (CXL) in a newly established in vivo murine model. METHODS: C57BL/6 wild-type mice (N = 67) were treated with various CXL protocols, with modification of the following parameters: total energy (fluence) used, duration of UV-A irradiation, continuous versus pulsed irradiation, and CXL under hypoxic conditions (contact lens). Corneas were evaluated biomicroscopically, histologically, and using optical coherence tomography...
January 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/28053663/utilization-of-xylose-by-engineered-strains-of-ashbya-gossypii-for-the-production-of-microbial-oils
#9
David Díaz-Fernández, Patricia Lozano-Martínez, Rubén M Buey, José Luis Revuelta, Alberto Jiménez
BACKGROUND: Ashbya gossypii is a filamentous fungus that is currently exploited for the industrial production of riboflavin. The utilization of A. gossypii as a microbial biocatalyst is further supported by its ability to grow in low-cost feedstocks, inexpensive downstream processing and the availability of an ease to use molecular toolbox for genetic and genomic modifications. Consequently, A. gossypii has been also introduced as an ideal biotechnological chassis for the production of inosine, folic acid, and microbial oils...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28009397/corneal-collagen-crosslinking-for-corneal-ectasias-a-review
#10
David P S O'Brart
PURPOSE: To review the published literature on corneal collagen crosslinking (CXL). METHODS: Importance has been placed on seminal publications, systemic reviews, meta-analyses, and randomized controlled clinical trials. Where such evidence was not available, cohort studies, case-controlled studies, and case series with follow-up greater than 12 months were examined. RESULTS: Corneal collagen crosslinking with riboflavin and ultraviolet A (UVA) 370 nm radiation appears to be capable of arresting the progression of ectatic corneal disorders, with most studies reporting significant improvements in visual, keratometric, and topographic measurements...
December 6, 2016: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28008713/engineering-ashbya-gossypii-strains-for-de-novo-lipid-production-using-industrial-by-products
#11
Patricia Lozano-Martínez, Rubén M Buey, Rodrigo Ledesma-Amaro, Alberto Jiménez, José Luis Revuelta
Ashbya gossypii is a filamentous fungus that naturally overproduces riboflavin, and it is currently exploited for the industrial production of this vitamin. The utilization of A. gossypii for biotechnological applications presents important advantages such as the utilization of low-cost culture media, inexpensive downstream processing and a wide range of molecular tools for genetic manipulation, thus making A. gossypii a valuable biotechnological chassis for metabolic engineering. A. gossypii has been shown to accumulate high levels of lipids in oil-based culture media; however, the lipid biosynthesis capacity is rather limited when grown in sugar-based culture media...
March 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/27830356/successful-treatment-of-a-patient-with-ethylmalonic-encephalopathy-by-intravenous-n-acetylcysteine
#12
Mustafa Kılıç, Özge Dedeoğlu, Rahşan Göçmen, Selman Kesici, Deniz Yüksel
Ethylmalonic encephalopathy (EE) is an autosomal recessive devastating metabolic disorder affecting the brain, gastrointestinal tract, peripheral vessels and rarely the other vascular organs. We report a 10-month-old girl who presented as a meningococcemia clinic but later diagnosed ethylmalonic encephalopathy. Molecular analyses revealed a homozygous c.554 T > G; p. L185R mutation in ETHE1 gene. She was only partially benefited from riboflavine, coenzyme Q10, metronidazole, N-acetylcysteine and symptomatic treatment and discharged from hospital with the sequela of oxygene dependance and developmental delay...
April 2017: Metabolic Brain Disease
https://www.readbyqxmd.com/read/27791453/new-biotechnological-applications-for-ashbya-gossypii-challenges-and-perspectives
#13
Tatiana Q Aguiar, Rui Silva, Lucília Domingues
The filamentous fungus Ashbya gossypii has long been considered a paradigm of the White Biotechnology in what concerns riboflavin production. Its industrial relevance led to the development of a significant molecular and in silico modeling toolbox for its manipulation. This, together with the increasing knowledge of its genome and metabolism has helped designing effective metabolic engineering strategies for optimizing riboflavin production, but also for developing new A. gossypii strains for novel biotechnological applications, such as production of recombinant proteins, single cell oils (SCOs), and flavour compounds...
October 28, 2016: Bioengineered
https://www.readbyqxmd.com/read/27790197/the-nitrogen-availability-interferes-with-mycorrhiza-induced-resistance-against-botrytis-cinerea-in-tomato
#14
Paloma Sanchez-Bel, Pilar Troncho, Jordi Gamir, Maria J Pozo, Gemma Camañes, Miguel Cerezo, Víctor Flors
Mycorrhizal plants are generally quite efficient in coping with environmental challenges. It has been shown that the symbiosis with arbuscular mycorrhizal fungi (AMF) can confer resistance against root and foliar pathogens, although the molecular mechanisms underlying such mycorrhiza-induced resistance (MIR) are poorly understood. Tomato plants colonized with the AMF Rhizophagus irregularis display enhanced resistance against the necrotrophic foliar pathogen Botrytis cinerea. Leaves from arbuscular mycorrhizal (AM) plants develop smaller necrotic lesions, mirrored also by a reduced levels of fungal biomass...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27757515/high-throughput-and-simultaneous-quantitative-analysis-of-homocysteine-methionine-cycle-metabolites-and-co-factors-in-blood-plasma-and-cerebrospinal-fluid-by-isotope-dilution-lc-ms-ms
#15
Seu Ping Guiraud, Ivan Montoliu, Laeticia Da Silva, Loïc Dayon, Antonio Núñez Galindo, John Corthésy, Martin Kussmann, Francois-Pierre Martin
The methionine cycle is a key pathway contributing to the regulation of human health, with well-established involvement in cardiovascular diseases and cognitive function. Changes in one-carbon cycle metabolites have also been associated with mild cognitive decline, vascular dementia, and Alzheimer's disease. Today, there is no single analytical method to monitor both metabolites and co-factors of the methionine cycle. To address this limitation, we here report for the first time a new method for the simultaneous quantitation of 17 metabolites in the methionine cycle, which are homocysteic acid, taurine, serine, cysteine, glycine, homocysteine, riboflavin, methionine, pyridoxine, cystathionine, pyridoxamine, S-adenosylhomocysteine, S-adenosylmethionine, betaine, choline, dimethylglycine, and 5-methyltetrahydrofolic acid...
January 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27709903/in-situ-molecular-imaging-of-the-biofilm-and-its-matrix
#16
Yuanzhao Ding, Yufan Zhou, Juan Yao, Craig Szymanski, James Fredrickson, Liang Shi, Bin Cao, Zihua Zhu, Xiao-Ying Yu
Molecular mapping of live biofilms at submicrometer resolution presents a grand challenge. Here, we present the first chemical mapping results of biofilm extracellular polymeric substance (EPS) in biofilms using correlative imaging between super resolution fluorescence microscopy and liquid time-of-flight secondary ion mass spectrometry (TOF-SIMS). Shewanella oneidensis is used as a model organism. Heavy metal chromate (Cr2O7(2-)) anions consisting of chromium Cr(VI) was used as a model environmental stressor to treat the biofilms...
November 15, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27620349/vitamin-b2-as-a-virulence-factor-in-pseudogymnoascus-destructans-skin-infection
#17
Miroslav Flieger, Hana Bandouchova, Jan Cerny, Milada Chudíčková, Miroslav Kolarik, Veronika Kovacova, Natália Martínková, Petr Novák, Ondřej Šebesta, Eva Stodůlková, Jiri Pikula
Pathogenic and non-pathogenic related microorganisms differ in secondary metabolite production. Here we show that riboflavin overproduction by a fungal pathogen and its hyperaccumulation in affected host tissue exacerbates a skin infection to necrosis. In white-nose syndrome (WNS) skin lesions caused by Pseudogymnoascus destructans, maximum riboflavin concentrations reached up to 815 μg ml(-1), indicating bioaccumulation and lack of excretion. We found that high riboflavin concentrations are cytotoxic under conditions specific for hibernation, affect bats' primary fibroblasts and induce cell detachment, loss of mitochondrial membrane potential, polymerization of cortical actin, and cell necrosis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27605716/alkaline-stress-and-iron-deficiency-regulate-iron-uptake-and-riboflavin-synthesis-gene-expression-differently-in-root-and-leaf-tissue-implications-for-iron-deficiency-chlorosis
#18
En-Jung Hsieh, Brian M Waters
Iron (Fe) is an essential mineral that has low solubility in alkaline soils, where its deficiency results in chlorosis. Whether low Fe supply and alkaline pH stress are equivalent is unclear, as they have not been treated as separate variables in molecular physiological studies. Additionally, molecular responses to these stresses have not been studied in leaf and root tissues simultaneously. We tested how plants with the Strategy I Fe uptake system respond to Fe deficiency at mildly acidic and alkaline pH by measuring root ferric chelate reductase (FCR) activity and expression of selected Fe uptake genes and riboflavin synthesis genes...
October 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27591616/surface-enhanced-raman-spectroscopy-sers-in-food-analytics-detection-of-vitamins-b2-and-b12-in-cereals
#19
Andreea Ioana Radu, Maria Kuellmer, Bernd Giese, Uwe Huebner, Karina Weber, Dana Cialla-May, Jürgen Popp
Food analysis has been gaining interest throughout recent decades for different reasons: the detection of hazardous substances in food and routine investigations of food composition and vitamin/nutrient contents. Regardless of the targeted component, food analysis raises a few challenges regarding the complexity of the matrix and detecting trace amounts of substances. We report herein the results obtained regarding the simultaneous detection of two B vitamins (riboflavin, vitamin B2 and cyanocobalamin, vitamin B12) by means of SERS...
November 1, 2016: Talanta
https://www.readbyqxmd.com/read/27576102/hyaluronan-cholesterol-nanohydrogels-characterisation-and-effectiveness-in-carrying-alginate-lyase
#20
Elita Montanari, Chiara Di Meo, Simona Sennato, Antonio Francioso, Anna Laura Marinelli, Francesca Ranzo, Serena Schippa, Tommasina Coviello, Federico Bordi, Pietro Matricardi
Although in recent years several methods have been studied and developed to obtain different types of nanosized drug delivery systems, the set up of suitable procedures and materials remains highly expensive, their preparation is time consuming and often not feasible for a scale-up process. Furthermore, the sterilisation and storage of nanocarrier formulations represents a complicated but mandatory step for their effective use. In our previous work we assessed the use of an autoclaving process to achieve, in one simple step, sterile self-assembled hyaluronan-cholesterol (HA-CH) and hyaluronan-riboflavin (HA-Rfv) nanohydrogels (NHs)...
July 25, 2017: New Biotechnology
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