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Riboflavin molecular

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https://www.readbyqxmd.com/read/29338178/dna-damage-and-apoptosis-induction-in-cancer-cells-by-chemically-engineered-thiolated-riboflavin-gold-nanoassembly
#1
Abhishek Sau, Sulagna Sanyal, Kallol Bera, Sabyasachi Sen, Amrit Krishna Mitra, Uttam Pal, Prabal Kumar Chakraborty, Sayantan Ganguly, Biswarup Satpati, Chandrima Das, Samita Basu
Herein we have engineered a smart nuclear targeting thiol-modified riboflavin-gold nano assembly, RfS@AuNPs, which accumulates selectively in nucleus without any nuclear-targeting peptides (NLS/RGD) and shows photophysically in vitro DNA intercalation. A theoretical model using Molecular Dynamics has been developed to probe the mechanism of formation and stability as well as dynamics of the RfS@AuNPs in aqueous solution and within DNA microenvironment. The RfS@AuNPs facilitate the binucleated cell formation that is reflected in the significant increase of DNA damage marker, γ-H2AX as well as the arrest of most of the HeLa cells at pre-G1 phase indicating cell death...
January 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29322794/integrated-analysis-of-the-rna-seq-data-of-liver-hepatocellular-carcinoma
#2
H Xue, L Luo, Y T Yao, L L Wei, S P Deng, X L Huang
The present study aimed to explore the genetic changes involved in the liver hepatocellular carcinoma (HCC) development. The RNA-Seq data of 212 HCC tissue samples and 50 normal tissue samples were downloaded using TCGA-Assembler. A total of 4 subgroups were obtained, and 4167, 6279, 5379, and 2548 DEGs were screened in group 1, group 2, group 3, and group 4, respectively. Enrichment analysis found that cell cycle, metabolism, and translation related terms were the most significantly changed functions and pathways...
2018: Neoplasma
https://www.readbyqxmd.com/read/29299926/hydrophilic-multi-template-molecularly-imprinted-biopolymers-based-on-a-green-synthesis-strategy-for-determination-of-b-family-vitamins
#3
Abbas Ostovan, Mehrorang Ghaedi, Maryam Arabi, Qian Yang, Jinhua Li, Lingxin Chen
A novel green synthesis strategy was proposed for preparation of multi-template molecularly imprinted biopolymers (mt-MIBP) in aqueous media with less consumption of organic solvents, which were subsequently used as sorbents of ultrasound-assisted dispersive solid-phase extraction (d-SPE) for simultaneous recognition and efficient separation of B-family vitamins in juice samples followed by high performance liquid chromatography (HPLC) determination. The obtained mt-MIBP was fully characterized by SEM, FT-IR, TEM and BET...
January 4, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29277443/priming-plant-resistance-by-activation-of-redox-sensitive-genes
#4
Carmen González-Bosch
Priming by natural compounds is an interesting alternative for sustainable agriculture, which also contributes to explore the molecular mechanisms associated with stress tolerance. Although hosts and stress types eventually determine the mode of action of plant-priming agents, it highlights that many of them act on redox signalling. These include vitamins thiamine, riboflavin and quercetin; organic acids like pipecolic, azelaic and hexanoic; volatile organic compounds such as methyl jasmonate; cell wall components like chitosans and oligogalacturonides; H2O2, etc...
December 22, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29242757/differential-gene-transcription-of-extracellular-matrix-components-in-response-to-in-vivo-corneal-crosslinking-cxl-in-rabbit-corneas
#5
Sabine Kling, Arthur Hammer, Emilio A Torres Netto, Farhad Hafezi
Purpose: We studied changes in gene transcription after corneal crosslinking (CXL) in the rabbit cornea in vivo and identified potential molecular signaling pathways. Methods: A total of 15 corneas of eight male New-Zealand-White rabbits were de-epithelialized and equally divided into five groups. Group 1 served as an untreated control. Groups 2 to 5 were soaked with 0.1% riboflavin for 20 minutes, which in Groups 3 to 5 was followed by UV-A irradiation at a fluence of 5...
December 2017: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/29212059/on-the-nature-of-solvatochromic-effect-the-riboflavin-absorption-spectrum-as-a-case-study
#6
Isabella Daidone, Andrea Amadei, Massimiliano Aschi, Laura Zanetti-Polzi
We present here the calculation of the absorption spectrum of riboflavin in acetonitrile and dimethyl sulfoxide using a hybrid quantum/classical approach, namely the perturbed matrix method, based on quantum mechanical calculations and molecular dynamics simulations. The calculated spectra are compared to the absorption spectrum of riboflavin previously calculated in water and to the experimental spectra obtained in all three solvents. The experimentally observed variations in the absorption spectra upon change of the solvent environment are well reproduced by the calculated spectra...
November 16, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29185933/severe-riboflavin-deficiency-induces-alterations-in-the-hepatic-proteome-of-starter-pekin-ducks
#7
Jing Tang, Maria A Hegeman, Jian Hu, Ming Xie, Wenbiao Shi, Yong Jiang, Vincent de Boer, Yuming Guo, Shuisheng Hou, Jaap Keijer
Suboptimal vitamin B2 status is encountered globally. Riboflavin deficiency depresses growth and results in a fatty liver. The underlying mechanisms remain to be established and an overview of molecular alterations is lacking. We investigated hepatic proteome changes induced by riboflavin deficiency to explain its effects on growth and hepatic lipid metabolism. In all, 360 1-d-old Pekin ducks were divided into three groups of 120 birds each, with twelve replicates and ten birds per replicate. For 21 d, the ducks were fed ad libitum a control diet (CAL), a riboflavin-deficient diet (RD) or were pair-fed with the control diet to the mean daily intake of the RD group (CPF)...
November 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/29136837/optical-molecular-analysis-using-office-flatbed-photo-scanner-new-approaches-and-solutions
#8
K V Oskolok, E V Shults, O V Monogarova, A A Chaplenko
The design and operations principles of a prototype of optical device based on an office flatbed photo scanner with a slide adapter are described in the paper. The developed device is intended for the analysis of liquid-phase samples by colorimetry, photometry, fluorimetry and nephelometry. Teflon cassettes designed for fixing cuvettes on glass plate of the scanner. Teflon wedge-shaped inserts for cuvettes have been developed to optimize the conditions of colorimetric analysis. These inserts allow varying the thickness of the absorbing layer of solutions...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29052988/ionic-strength-mediated-phase-transitions-of-surface-adsorbed-dna-on-single-walled-carbon-nanotubes
#9
Daniel P Salem, Xun Gong, Albert Tianxiang Liu, Volodymyr B Koman, Juyao Dong, Michael S Strano
Single-stranded DNA oligonucleotides have unique, and in some cases sequence-specific molecular interactions with the surface of carbon nanotubes that remain the subject of fundamental study. In this work, we observe and analyze a generic, ionic strength-mediated phase transition exhibited by over 25 distinct oligonucleotides adsorbed to single-walled carbon nanotubes (SWCNTs) in colloidal suspension. The phase transition occurs as monovalent salts are used to modify the ionic strength from 500 mM to 1 mM, causing a reversible reduction in the fluorescence quantum yield by as much as 90%...
November 7, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28970517/human-in-vitro-model-reveals-the-effects-of-collagen-cross-linking-on-keratoconus-pathogenesis
#10
Rabab Sharif, Jesper Hjortdal, Henrik Sejersen, Garett Frank, Dimitrios Karamichos
Keratoconus (KC) is a corneal thinning disorder that leads to severe vision impairment As opposed to corneal transplantation; corneal collagen crosslinking (CXL) is a relatively non-invasive procedure that leads to an increase in corneal stiffness. In order to evaluate the effect of CXL on human corneal stromal cells in vitro, we developed a 3-D in vitro CXL model, using primary Human corneal fibroblasts (HCFs) from healthy patients and Human Keratoconus fibroblasts (HKCs) from KC patients. Cells were plated on transwell polycarbonate membranes and stimulated by a stable vitamin C...
October 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28943110/therapies-for-mitochondrial-diseases-and-current-clinical-trials
#11
REVIEW
Ayman W El-Hattab, Ana Maria Zarante, Mohammed Almannai, Fernando Scaglia
Mitochondrial diseases are a clinically and genetically heterogeneous group of disorders that result from dysfunction of the mitochondrial oxidative phosphorylation due to molecular defects in genes encoding mitochondrial proteins. Despite the advances in molecular and biochemical methodologies leading to better understanding of the etiology and mechanism of these diseases, there are still no satisfactory therapies available for mitochondrial disorders. Treatment for mitochondrial diseases remains largely symptomatic and does not significantly alter the course of the disease...
September 18, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28932990/treatment-opportunities-in-patients-with-metabolic-myopathies
#12
REVIEW
Mette Cathrine Ørngreen, John Vissing
Metabolic myopathies are disorders affecting utilization of carbohydrates or fat in the skeletal muscle. Adult patients with metabolic myopathies typically present with exercise-induced pain, contractures or stiffness, fatigue, and myoglobinuria. Symptoms are related to energy failure. Purpose of review In this review, the current treatment options, including exercise therapy, dietary treatment, pharmacological supplementation, gene transcription, and enzyme replacement therapy, are described. Recent findings Recognition of the metabolic block in the metabolic myopathies has started the development of new therapeutic options...
September 21, 2017: Current Treatment Options in Neurology
https://www.readbyqxmd.com/read/28911109/selective-tumor-cell-death-induced-by-irradiated-riboflavin-through-recognizing-dna-g-t-mismatch
#13
Yi Yuan, Yongyun Zhao, Lianqi Chen, Jiasi Wu, Gangyi Chen, Sheng Li, Jiawei Zou, Rong Chen, Jian Wang, Fan Jiang, Zhuo Tang
Riboflavin (vitamin B2) has been thought to be a promising antitumoral agent in photodynamic therapy, though the further application of the method was limited by the unclear molecular mechanism. Our work reveals that riboflavin was able to recognize G-T mismatch specifically and induce single-strand breaks in duplex DNA targets efficiently under irradiation. In the presence of riboflavin, the photo-irradiation could induce the death of tumor cells that are defective in mismatch repair system selectively, highlighting the G-T mismatch as potential drug target for tumor cells...
September 6, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28821122/-click-hyaluronan-based-nanohydrogels-as-multifunctionalizable-carriers-for-hydrophobic-drugs
#14
Giuliana Manzi, Nicole Zoratto, Silvia Matano, Rocchina Sabia, Claudio Villani, Tommasina Coviello, Pietro Matricardi, Chiara Di Meo
Highly hydrophilic and biocompatible nanocarriers based on polysaccharide hydrogels (nanohydrogels, NHs) were shown to be promising systems for drug delivery applications. Following the idea of these emerging drug carriers, the aim of the present work was to develop self-assembled hydrogel nanoparticles based on amphiphilic derivatives of hyaluronic acid (HA) and riboflavin (Rfv), synthesized by "click" Copper(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC) reaction. The obtained amphiphilic product (HA-c-Rfv) was able to form nanohydrogels in aqueous environments, in particular by applying an innovative autoclave-based method...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28688841/how-mr1-presents-a-pathogen-metabolic-signature-to-mucosal-associated-invariant-t-mait-cells
#15
REVIEW
Hamish E G McWilliam, Jose A Villadangos
Mucosal-associated invariant T (MAIT) cells are innate-like lymphocytes restricted by the antigen (Ag)-presenting molecule MHC class I (MHC I)-related protein 1 (MR1). The Ags presented by MR1 are vitamin B-related Ags (VitBAgs), 'building-block' metabolites of riboflavin that are synthesized by a range of microbes. MR1 presentation is thus a unique mechanism for the immune detection of a pathogen metabolic signature. While the full picture of how MR1 accomplishes this remains incomplete, recent data show that, unlike other MHC molecules, MR1 operates by a presentation-on-demand mechanism...
July 5, 2017: Trends in Immunology
https://www.readbyqxmd.com/read/28665138/alternative-electron-transfer-channels-ensure-ultrafast-deactivation-of-light-induced-excited-states-in-riboflavin-binding-protein
#16
Laura Zanetti-Polzi, Massimiliano Aschi, Andrea Amadei, Isabella Daidone
Flavoproteins, containing flavin chromophores, are enzymes capable of transferring electrons at very high speeds. The ultrafast photoinduced electron-transfer (ET) kinetics of riboflavin binding protein to the excited riboflavin was studied by femtosecond spectroscopy and found to occur within a few hundred femtoseconds [ Zhong and Zewail, Proc. Natl. Acad. Sci. U.S.A. 2001, 98, 11867-11872 ]. This ultrafast kinetics was attributed to the presence of two aromatic rings that could transfer the electron to riboflavin: the side chains of tryptophan 156 and tyrosine 75...
July 7, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28654805/role-of-root-exudates-in-metal-acquisition-and-tolerance
#17
REVIEW
Yi-Tze Chen, Ying Wang, Kuo-Chen Yeh
Plants acquire mineral nutrients mostly through the rhizosphere; they secrete a large number of metabolites into the rhizosphere to regulate nutrient availability and to detoxify undesirable metal pollutants in soils. The secreted metabolites are inorganic ions, gaseous molecules, and mainly carbon-based compounds. This review focuses on the mechanisms and regulation of low-molecular-weight organic-compound exudation in terms of metal acquisition. We summarize findings on riboflavin/phenolic-facilitated and phytosiderophore-facilitated iron acquisition and discuss recent studies of the functions and secretion mechanisms of low-molecular-weight organic acids in heavy-metal detoxification...
June 24, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28637675/structure-functional-aspects-of-the-human-riboflavin-transporter-3-slc52a3-role-of-the-predicted-glycosylation-and-substrate-interacting-sites
#18
Veedamali S Subramanian, Subrata Sabui, Trevor Teafatiller, Jennifer A Bohl, Hamid M Said
The human riboflavin (RF) transporter-3 (hRFVT-3; product of the SLC52A3 gene) plays an essential role in the intestinal RF absorption process and is expressed exclusively at the apical membrane domain of polarized enterocytes. Previous studies have characterized different physiological/biological aspects of this transporter, but nothing is known about the glycosylation status of the hRFVT-3 protein and role of this modification in its physiology/biology. Additionally, little is known about the residues in the hRFVT-3 protein that interact with the ligand, RF...
August 1, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28620369/characterization-of-bile-salt-hydrolase-from-lactobacillus-gasseri-fr4-and-demonstration-of-its-substrate-specificity-and-inhibitory-mechanism-using-molecular-docking-analysis
#19
Rizwana Parveen Rani, Marimuthu Anandharaj, Abraham David Ravindran
Probiotic bacteria are beneficial to the health of poultry animals, thus are used as alternative candidates for antibiotics used as growth promoters (AGPs). However, they also reduce the body weight gain due to innate bile salt hydrolase (BSH) activity. Hence, the addition of a suitable BSH inhibitor along with the probiotic feed can decrease the BSH activity. In this study, a BSH gene (981 bp) encoding 326-amino acids was identified from the genome of Lactobacillus gasseri FR4 (LgBSH). The LgBSH-encoding gene was cloned and purified using an Escherichia coli BL21 (DE3) expression system, and its molecular weight (37 kDa) was confirmed by SDS-PAGE and a Western blot analysis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28572726/photoacoustic-imaging-of-tumor-targeting-with-riboflavin-functionalized-theranostic-nanocarriers
#20
Nataliia Beztsinna, Yoanna Tsvetkova, Jithin Jose, Boutayna Rhourri-Frih, Wa'el Al Rawashdeh, Twan Lammers, Fabian Kiessling, Isabelle Bestel
Photoacoustic imaging is an emerging method in the molecular imaging field, providing high spatiotemporal resolution and sufficient imaging depths for many clinical applications. Therefore, the aim of this study was to use photoacoustic imaging as a tool to evaluate a riboflavin (RF)-based targeted nanoplatform. RF is internalized by the cells through a specific pathway, and its derivatives were recently shown as promising tumor-targeting vectors for the drug delivery systems. Here, the RF amphiphile synthesized from a PEGylated phospholipid was successfully inserted into a long-circulating liposome formulation labeled with the clinically approved photoacoustic contrast agent - indocyanine green (ICG)...
2017: International Journal of Nanomedicine
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