Miller Huang, Wanqi Fang, Alvin Farrel, Linwei Li, Antonios Chronopoulos, Nicole Nasholm, Bo Cheng, Tina Zheng, Hiroyuki Yoda, Megumi J Barata, Tania Porras, Matthew L Miller, Qiqi Zhen, Lisa Ghiglieri, Lauren McHenry, Linyu Wang, Shahab Asgharzadeh, JinSeok Park, W Clay Gustafson, Katherine K Matthay, John M Maris, William A Weiss
Neuroblastoma is the most common extracranial solid tumor of childhood. While MYCN and mutant anaplastic lymphoma kinase (ALKF1174L ) cooperate in tumorigenesis, how ALK contributes to tumor formation remains unclear. Here, we used a human stem cell-based model of neuroblastoma. Mis-expression of ALKF1174L and MYCN resulted in shorter latency compared to MYCN alone. MYCN tumors resembled adrenergic, while ALK/MYCN tumors resembled mesenchymal, neuroblastoma. Transcriptomic analysis revealed enrichment in focal adhesion signaling, particularly the extracellular matrix genes POSTN and FN1 in ALK/MYCN tumors...
March 5, 2024: Cell Reports