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leptospira in dogs

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https://www.readbyqxmd.com/read/29768596/sociodemographic-and-environmental-analysis-for-the-occurrence-of-anti-leptospira-antibodies-in-dogs-of-teresina-piau%C3%A3-brazil
#1
Elís Rosélia Dutra de Freitas Siqueira Silva, Vanessa Castro, Ana Lys Bezerra Barradas Mineiro, Maria das Graças Prianti, Gustavo Henrique Chaves Martins, Misael das Virgens Santana, Lucas Moreira Brito, Silvana Maria Medeiros de Sousa Silva
Leptospirosis is a worldwide zoonosis whose transmission is interlinked by multiple factors in the man-animal-ecosystem interface. This study aimed to evaluate the risk factors for the occurrence of anti-Leptospira antibodies in dogs in the capital Teresina (PI), and to determine their spatial distribution. Five hundred fifty-eight dog blood samples were submitted to the Microscopic Serum Agglutination (MSA) test. We applied semi-structured questionnaires to dog owners and obtained the area of residence for projection in geographical maps...
May 2018: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/29768448/urinary-shedding-of-pathogenic-leptospira-in-stray-dogs-and-cats-algiers-a-prospective-study
#2
Sara Zaidi, Amar Bouam, Amina Bessas, Djamila Hezil, Hicham Ghaoui, Khatima Ait-Oudhia, Michel Drancourt, Idir Bitam
BACKGROUND: Leptospirosis is an important worldwide zoonosis. This disease is caused by pathogenic species of the genus Leptospira which are maintained in the environment via chronic renal infection of carrier animals which can be asymptomatic excretors of the organisms in their urines and become a source of infection for humans and other hosts. The prevalence of animal leptospirosis in Algiers, Algeria, is unknown. METHODOLOGY/PRINCIPAL FINDINGS: Real-time PCR and standard PCR and sequencing were used to detect pathogenic Leptospira organisms in the urines of stray dogs and cats in Algiers...
2018: PloS One
https://www.readbyqxmd.com/read/29649858/pharmacokinetics-of-enrofloxacin-hcl-2h-2-o-enro-c-in-dogs-and-pk-pd-monte-carlo-simulations-against-leptospira-sp
#3
Hector Sumano, Luis Ocampo, Graciela Tapia, C de Jesus Mendoza, Lilia Gutierrez
Pharmacokinetics/pharmacodynamics (PK/PD) ratios of reference enrofloxacin (Enro-R) and enrofloxacin as HCl-2H2 O (Enro-C), as well as Monte Carlo simulations based on composite MIC50 and MIC90 vs. Leptospira sp., were carried out in dogs after their IM and oral administration (10 mg/kg). Plasma determination of enrofloxacin was achieved by means of high performance liquid chromatography (HPLC). Maximum plasma concentration values after oral administration were 1.47 ± 0.19 µg/mL and 5.3 ± 0.84 µg/mL for Enro-R and Enro-C, respectively, and 1...
April 12, 2018: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29525230/seroprevalence-of-leptospirosis-in-working-dogs
#4
S F Lau, J Y Wong, K H Khor, M A Roslan, M S Abdul Rahman, S K Bejo, R Radzi, A R Bahaman
Working dogs are canine animals that have been trained to assist human beings in carrying out various tasks. They help in guarding property, performing rescues, assisting the visually impaired or physically handicapped, searching for drugs, explosives, and others. Leptospirosis is one of the most widespread zoonotic diseases in the world and a commonly occurring disease of the tropics and subtropics. In Malaysia, all working dogs are normally vaccinated with serovars, Pomona, Icterohaemorrhagiae, Canicola, and Grippotyphosa based on protocols recommended from other countries...
December 2017: Topics in Companion Animal Medicine
https://www.readbyqxmd.com/read/29459489/fifteen-month-duration-of-immunity-for-the-serovar-grippotyphosa-fraction-of-a-tetravalent-canine-leptospirosis-vaccine
#5
Deborah A Grosenbaugh, Maria Camila Pardo
Forty-four specific pathogen-free beagles, median age 65 days, received two subcutaneous doses of either a commercially available, five-way combination vaccine or the same vaccine in combination with a tetravalent Leptospira bacterin (Canicola, Grippotyphosa, Icterohaemorrhagiae, Pomona). They were subsequently challenged with a pathogenic strain L kirschneri serovar Grippotyphosa 470 days following completion of the vaccination protocol. Titres of agglutinating serum antibodies were determined at various time points before and after both vaccination and challenge, along with postchallenge reisolation of the challenge organisms from blood and urine, and evaluation of renal histopathology...
February 19, 2018: Veterinary Record
https://www.readbyqxmd.com/read/29371342/draft-genome-sequence-of-a-leptospira-interrogans-strain-isolated-from-the-urine-of-an-asymptomatic-dog-in-thailand
#6
Alongkorn Kurilung, Chantisa Keeratipusana, Prapat Suriyaphol, Nuvee Prapasarakul
In 2014, Leptospira interrogans strain CUDO8 was isolated from the urine of an asymptomatic dog in Thailand. Here we report the draft genome sequence of this pathogenic bacterium.
January 25, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29279785/-leptospira-seroprevalence-in-domestic-dogs-and-cats-on-the-caribbean-island-of-saint-kitts
#7
Nicola Pratt, Anne Conan, Sreekumari Rajeev
Leptospirosis is an important bacterial zoonotic disease that affects humans and many animal species. Knowledge of prevalence of Leptospira in a given geographic region supports the implementation of effective control/prevention programmes and thus reduces the transmission risks. This study provides Leptospira seroprevalence and serovar distribution in dogs and cats on the Caribbean island of Saint Kitts. Convenient serum samples from domestic dogs ( n = 101) and cats ( n = 50) were tested by the microscopic agglutination test (MAT) using 21 Leptospira serovars belonging to 17 serogroups...
2017: Veterinary Medicine International
https://www.readbyqxmd.com/read/29236931/comparative-genomics-of-pathogenic-leptospira-interrogans-serovar-canicola-isolated-from-swine-and-human-in-brazil
#8
COMPARATIVE STUDY
Luisa Z Moreno, Fabiana Miraglia, Frederico S Kremer, Marcus R Eslabao, Odir A Dellagostin, Walter Lilenbaum, Julio C Freitas, Silvio A Vasconcellos, Marcos B Heinemann, Andrea M Moreno
Leptospira interrogans serovar Canicola is one of the most important pathogenic serovars for the maintenance of urban leptospirosis. Even though it is considered highly adapted to dogs, serovar Canicola infection has already been described in other animals and even a few human cases. Here, we present the genomic characterisation of two Brazilian L. interrogans serovar Canicola strains isolated from slaughtered sows (L0-3 and L0-4) and their comparison with human strain Fiocruz LV133. It was observed that the porcine serovar Canicola strains present the genetic machinery to cause human infection and, therefore, represent a higher risk to public health...
February 2018: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/29233483/development-and-validation-of-a-modified-taqman-based-real-time-pcr-assay-targeting-the-lipl32-gene-for-detection-of-pathogenic-leptospira-in-canine-urine-samples
#9
Bruno Alonso Miotto, Aline Santana da Hora, Sueli Akemi Taniwaki, Paulo Eduardo Brandão, Marcos Bryan Heinemann, Mitika Kuribayashi Hagiwara
A modified TaqMan real-time polymerase chain reaction targeting a 138bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy dogs were tested for presence of leptospiral DNA using the TaqMan-based assay. The results were compared with those from a well-established conventional PCR targeting the 16S RNA encoding gene associated with nucleotide sequencing analysis...
December 2, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29188067/isolation-and-characterization-of-leptospira-interrogans-serovar-copenhageni-from-a-dog-from-saint-kitts
#10
Christopher R Larson, Michelle Dennis, Rajeev V Nair, Alejandro Llanes, Andrea Peda, Shamara Welcome, Sreekumari Rajeev
Introduction. Leptospirosis is a zoonotic bacterial disease of global distribution affecting humans and animals. The initial phase of leptospirosis resembles many other febrile illness and due to its broad and biphasic clinical manifestations, selection and implementation of appropriate diagnostic tests can be challenging. Case presentation. This report describes a case investigation of a 14 weeks old male, orphan puppy, presented with generalised jaundice, anemia, weakness, and anorexia. Clinical abnormalities included the evidence of renal and hepatic failure...
October 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29131400/evaluation-of-3-serological-tests-for-early-detection-of-leptospira-specific-antibodies-in-experimentally-infected-dogs
#11
J Lizer, S Velineni, A Weber, M Krecic, P Meeus
BACKGROUND: Leptospirosis in dogs is a disease of global importance. Early detection and appropriate therapeutic intervention are necessary to resolve infection and prevent zoonotic transmission. However, its diagnosis is hindered by nonspecific clinical signs and lack of rapid diagnostic tests of early infection. Recently, 2 rapid point-of-care tests (WITNESS Lepto [WITNESS Lepto, Zoetis LLC, Kalamazoo, MI, USA] and SNAP Lepto [SNAP Lepto, IDEXX Laboratories, Westbrook, ME, USA]) for detection of Leptospira-specific antibodies in canine sera were developed...
January 2018: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29067208/serological-survey-of-leptospira-interrogans-toxoplasma-gondii-and-trypanosoma-cruzi-in-free-roaming-domestic-dogs-and-cats-from-a-marginated-rural-area-of-yucatan-mexico
#12
Antonio Ortega-Pacheco, Eugenia Guzmán-Marín, Karla Y Acosta-Viana, Ignacio Vado-Solís, Bertha Jiménez-Delgadillo, Maria Cárdenas-Marrufo, Carlos Pérez-Osorio, Marilyn Puerto-Solís, Matilde Jiménez-Coello
To evaluate the serological status for Trypanosoma cruzi , Toxoplasma gondii and Leptospira interrogans antibodies in free roaming dogs and cats from a marginated rural community in Yucatan Mexico, 100 households were visited and animals sampled. From the 106 samples, 93 were from dogs and 13 were from cats. Frequency of positive results for T. gondii, T. cruzi and Leptospira spp was 97.8%, 9.7% and 45.2% for dogs and 92.3%, 0.0% and 15.2% for cats, respectively. No associations with age, sex and body condition was found for T...
February 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/28861677/seroepidemiological-survey-on-leptospira-spp-infection-in-wild-and-domestic-mammals-in-two-distinct-areas-of-the-semi-arid-region-of-northeastern-brazil
#13
Laís Ferrari Dos Santos, Maíra Freitas Guimarães, Gisele Oliveira de Souza, Ivo Wesley Gomes da Silva, Josenilton Rodrigues Santos, Sérgio Santos Azevedo, Marcelo Bahia Labruna, Marcos Bryan Heinemann, Mauricio Claudio Horta
Leptospirosis is a bacterial zoonotic disease that causes severe reproductive problems in livestock and generates economic losses for farmers. This study aimed to determine the seroprevalence of anti-Leptospira antibodies in small mammals, both wild and domestic, in two distinct areas of the semi-arid region of northeastern Brazil: the National Park of Serra das Confusões (NPSC), state of Piauí, a preserved area; and rural areas in the municipalities of Petrolina and Lagoa Grande, state of Pernambuco, non-preserved areas...
December 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28803792/rapid-and-visual-detection-of-leptospira-in-urine-by-ligb-lamp-assay-with-pre-addition-of-dye
#14
Syed Atif Ali, Gurpreet Kaur, Nongthombam Boby, T Sabarinath, Khushal Solanki, Dheeraj Pal, Pallab Chaudhuri
Leptospirosis is considered to be the most widespread zoonotic disease caused by pathogenic species of Leptospira. The present study reports a novel set of primers targeting LigB gene for visual detection of pathogenic Leptospira in urine samples through Loop-mediated isothermal amplification (LAMP). The results were recorded by using Hydroxyl napthol blue (HNB), SYBR GREEN I and calcein. Analytical sensitivity of LAMP was as few as 10 leptospiral organisms in spiked urine samples from cattle and dog. LigB gene based LAMP, termed as LigB-LAMP, was found 10 times more sensitive than conventional PCR...
December 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28782459/leptospira-spp-vaccinal-antibodies-do-not-react-with-borrelia-burgdorferi-peptides-used-in-the-accuplex-4
#15
Amber L Caress, Scott Moroff, Michael R Lappin
We attempted to determine if Leptospira spp. antibodies induced by vaccination would cross-react with Borrelia burgdorferi antigens used in a commercial automated immunofluorescent assay (AccuPlex 4 BioCD; Antech). Staff- and student-owned dogs ( n = 31) were recruited at a veterinary teaching hospital in a B. burgdorferi nonendemic area. The dogs were randomized and administered 1 of 4 commercial Leptospira spp. vaccines that contained serovars Canicola, Grippotyphosa, Icterohaemorrhagiae, and Pomona, then booster vaccinated 3 wk later...
November 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28743546/human-and-animal-leptospirosis-in-southern-brazil-a-five-year-retrospective-study
#16
Sérgio Jorge, Rodrigo Andrade Schuch, Natasha Rodrigues de Oliveira, Carlos Eduardo Pouey da Cunha, Charles Klazer Gomes, Thais Larré Oliveira, Caroline Rizzi, Aisha Farid Qadan, Violetta Dias Pacce, Ana Lúcia Coelho Recuero, Claudiomar Soares Brod, Odir Antônio Dellagostin
BACKGROUND: Leptospirosis is an emerging zoonosis attributed to multiple reservoirs. Climatic conditions influence the transmission of pathogenic leptospires, which require warm and humid conditions for survival. The influence of seasonality in human and animal leptospirosis in the subtropical region of Brazil remains poorly understood. METHODS: We performed a retrospective study to describe the patterns of human and animal exposure to leptospirosis and their association with precipitation events in Southern Brazil...
July 2017: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/28722568/epidemiology-of-spotted-fever-group-rickettsioses-and-acute-undifferentiated-febrile-illness-in-villeta-colombia
#17
Álvaro A Faccini-Martínez, Alejandro Ramírez-Hernández, Christian Barreto, Elkin Forero-Becerra, Diego Millán, Elkin Valbuena, Andrea C Sánchez-Alfonso, Wilson O Imbacuán-Pantoja, Jesús A Cortés-Vecino, Luis J Polo-Terán, Néstor Yaya-Lancheros, Jorge Jácome, Ana M Palomar, Sonia Santibáñez, Aránzazu Portillo, José A Oteo, Marylin Hidalgo
Etiology of acute undifferentiated febrile syndrome (AUFS) is often unknown, leading to inaccurate diagnosis and treatment. Villeta town has been identified as an endemic area for spotted fever group (SFG) rickettsioses but little is known about possible amplifier hosts and other Rickettsia species different from Rickettsia rickettsii. Besides, few studies have approached other AUFS etiologies in the region. We investigated the role of dengue, leptospirosis, rickettsioses, human anaplasmosis, and Q fever as possible causes of AUFS in patients from Villeta...
September 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28660382/epidemiology-behavior-of-leptospirosis-in-ci%C3%A3-naga-de-oro-c%C3%A3-rdoba-colombia
#18
C Ensuncho-Hoyos, V Rodríguez-Rodríguez, A Pérez-Doria, O Vergara, A Calderón-Rangel
The purpose of this study was to determine the epidemiology of leptospirosis in rural areas of Ciénaga de Oro, Córdoba, Colombia, a convenience sampling was carried out on 13 farms. The sample size was 325 reproductive age cows, 11 canine samples, and 20 humans. The samples were subjected to MAT analysis with 11 serogroups of Leptospira interrogans sensu lato. Once the MAT results were received, urine samples were collected from 78 cows, along with 39 water samples, for bacteriological cultures and PCR for the 16S rRNA gene in L...
October 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28658269/leptospira-diversity-in-animals-and-humans-in-tahiti-french-polynesia
#19
Vanina Guernier, Vaea Richard, Tuxuan Nhan, Eline Rouault, Anita Tessier, Didier Musso
BACKGROUND: Leptospirosis is a highly endemic bacterial zoonosis in French Polynesia (FP). Nevertheless, data on the epidemiology of leptospirosis in FP are scarce. We conducted molecular studies on Leptospira isolated from humans and the potential main animal reservoirs in order to identify the most likely sources for human infection. METHODOLOGY/PRINCIPAL FINDINGS: Wild rats (n = 113), farm pigs (n = 181) and domestic dogs (n = 4) were screened for Leptospira infection in Tahiti, the most populated island in FP...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28620258/development-of-indirect-enzyme-linked-immunosorbent-assay-for-diagnosis-of-canine-leptospirosis
#20
A Sathiyamoorthy, G Selvaraju, K M Palanivel, And P Srinivasan
AIM: This study was taken up to develop an indirect enzyme-linked immunosorbent assay (i-ELISA) for screening antibodies against Leptospira spp. in canines. MATERIALS AND METHODS: An i-ELISA was developed using outer membrane protein extracted from Leptospira interrogans serovar canicola used for coating the well with concentration of 0.5 µg/µl. A total of 250 serum samples from clinically affected and apparently healthy dogs were collected along with relevant epidemiological data at Teaching Veterinary Clinical Complex, Veterinary College and Research Institute, Namakkal, and subjected to i-ELISA...
May 2017: Veterinary World
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