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https://www.readbyqxmd.com/read/28441451/two-featured-series-of-rrna-derived-rna-fragments-rrfs-constitute-a-novel-class-of-small-rnas
#1
Ze Chen, Yu Sun, Xiaojun Yang, Zhenfeng Wu, Kaifei Guo, Xiaoran Niu, Qingsong Wang, Jishou Ruan, Wenjun Bu, Shan Gao
In this study, we reported two featured series of rRNA-derived RNA fragments (rRFs) from the small RNA sequencing (sRNA-seq) data of Amblyomma testudinarium using the Illunima platform. Two series of rRFs (rRF5 and rRF3) were precisely aligned to the 5' and 3' ends of the 5.8S and 28S rRNA gene. The rRF5 and rRF3 series were significantly more highly expressed than the rRFs located in the body of the rRNA genes. These series contained perfectly aligned reads, the lengths of which varied progressively with 1-bp differences...
2017: PloS One
https://www.readbyqxmd.com/read/28440879/tick-borne-encephalitis-in-patients-vaccinated-against-this-disease
#2
S Lotrič-Furlan, P Bogovič, T Avšič-Županc, M Jelovšek, L Lusa, F Strle
BACKGROUND: Information on tick-borne encephalitis (TBE) in patients already vaccinated against the disease is limited. OBJECTIVES: To compare the course and outcome in patients with vaccination breakthrough TBE with findings in patients who developed TBE without previous vaccination. METHODS: All adult patients diagnosed with TBE at a single medical centre during a 16-year period and who had received at least two doses of TBE vaccine before the onset of illness qualified for the study...
April 25, 2017: Journal of Internal Medicine
https://www.readbyqxmd.com/read/28439499/tick-pathogen-interactions-and-vector-competence-identification-of-molecular-drivers-for-tick-borne-diseases
#3
REVIEW
José de la Fuente, Sandra Antunes, Sarah Bonnet, Alejandro Cabezas-Cruz, Ana G Domingos, Agustín Estrada-Peña, Nicholas Johnson, Katherine M Kocan, Karen L Mansfield, Ard M Nijhof, Anna Papa, Nataliia Rudenko, Margarita Villar, Pilar Alberdi, Alessandra Torina, Nieves Ayllón, Marie Vancova, Maryna Golovchenko, Libor Grubhoffer, Santo Caracappa, Anthony R Fooks, Christian Gortazar, Ryan O M Rego
Ticks and the pathogens they transmit constitute a growing burden for human and animal health worldwide. Vector competence is a component of vectorial capacity and depends on genetic determinants affecting the ability of a vector to transmit a pathogen. These determinants affect traits such as tick-host-pathogen and susceptibility to pathogen infection. Therefore, the elucidation of the mechanisms involved in tick-pathogen interactions that affect vector competence is essential for the identification of molecular drivers for tick-borne diseases...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28438938/a-tick-antivirulence-protein-potentiates-antibiotics-against-staphylococcus-aureus
#4
Nabil M Abraham, Lei Liu, Brandon L Jutras, Kristen Murfin, Ali Acar, Timur O Yarovinsky, Erica Sutton, Martin Heisig, Christine Jacobs-Wagner, Erol Fikrig
New strategies are needed to combat antibiotic resistance, especially against pathogens such as methicillin-resistant Staphylococcus aureus A tick antifreeze glycoprotein, IAFGP, possess potent anti-biofilm properties against a variety of clinical pathogens including S. aureus Synergy between IAFGP, or a peptide (P1) representative of a repeat region of the protein, with different antibiotics was assessed in vitro Antibiotics that synergized with either IAFPG or P1 were further evaluated in vivo using vertebrate and invertebrate infection models...
April 24, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28438912/effects-of-conservation-management-of-landscapes-and-vertebrate-communities-on-lyme-borreliosis-risk-in-the-united-kingdom
#5
REVIEW
Caroline Millins, Lucy Gilbert, Jolyon Medlock, Kayleigh Hansford, Des Ba Thompson, Roman Biek
Landscape change and altered host abundance are major drivers of zoonotic pathogen emergence. Conservation and biodiversity management of landscapes and vertebrate communities can have secondary effects on vector-borne pathogen transmission that are important to assess. Here we review the potential implications of these activities on the risk of Lyme borreliosis in the United Kingdom. Conservation management activities include woodland expansion, management and restoration, deer management, urban greening and the release and culling of non-native species...
June 5, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28438910/lyme-disease-ecology-in-a-changing-world-consensus-uncertainty-and-critical-gaps-for-improving-control
#6
REVIEW
A Marm Kilpatrick, Andrew D M Dobson, Taal Levi, Daniel J Salkeld, Andrea Swei, Howard S Ginsberg, Anne Kjemtrup, Kerry A Padgett, Per M Jensen, Durland Fish, Nick H Ogden, Maria A Diuk-Wasser
Lyme disease is the most common tick-borne disease in temperate regions of North America, Europe and Asia, and the number of reported cases has increased in many regions as landscapes have been altered. Although there has been extensive work on the ecology and epidemiology of this disease in both Europe and North America, substantial uncertainty exists about fundamental aspects that determine spatial and temporal variation in both disease risk and human incidence, which hamper effective and efficient prevention and control...
June 5, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28438220/anaplasma-spp-in-dogs-and-owners-in-north-western-morocco
#7
Sarah Elhamiani Khatat, Sylvie Daminet, Malika Kachani, Christian M Leutenegger, Luc Duchateau, Hamid El Amri, Mony Hing, Rahma Azrib, Hamid Sahibi
BACKGROUND: Anaplasma phagocytophilum is an emerging tick-borne zoonotic pathogen of increased interest worldwide which has been detected in northern Africa. Anaplasma platys is also present in this region and could possibly have a zoonotic potential. However, only one recent article reports on the human esposure to A. phagocytophilum in Morocco and no data are available on canine exposure to both bacteria. Therefore, we conducted a cross-sectional epidemiological study aiming to assess both canine and human exposure to Anaplasma spp...
April 24, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28438088/anaplasma-ehrlichia-and-rickettsia-species-infections-in-cats
#8
Maria Grazia Pennisi, Regina Hofmann-Lehmann, Alan D Radford, Séverine Tasker, Sándor Belák, Diane D Addie, Corine Boucraut-Baralon, Herman Egberink, Tadeusz Frymus, Tim Gruffydd-Jones, Katrin Hartmann, Marian C Horzinek, Margaret J Hosie, Albert Lloret, Hans Lutz, Fulvio Marsilio, Etienne Thiry, Uwe Truyen, Karin Möstl
Anaplasma species, Ehrlichia species and Rickettsia species are vector-borne pathogens infecting a wide variety of mammals, but causing disease in very few of them. Infection in cats: Anaplasma phagocytophilum is the most important feline pathogen among these rickettsial organisms, and coinfections are possible. Little information is available on the pathogenesis of these agents in cats. Clinical signs are usually reported soon after tick infestation. They are mostly non-specific, consisting of fever, anorexia and lethargy...
May 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28435891/detection-of-anaplasma-phagocytophilum-babesia-microti-borrelia-burgdorferi-borrelia-miyamotoi-and-powassan-virus-in-ticks-by-a-multiplex-real-time-reverse-transcription-pcr-assay
#9
Rafal Tokarz, Teresa Tagliafierro, D Moses Cucura, Ilia Rochlin, Stephen Sameroff, W Ian Lipkin
Ixodes scapularis ticks are implicated in transmission of Anaplasma phagocytophilum, Borrelia burgdorferi, Borrelia miyamotoi, Babesia microti, and Powassan virus. We describe the establishment and implementation of the first multiplex real-time PCR assay with the capability to simultaneously detect and differentiate all five pathogens in a single reaction. The application of this assay for analysis of ticks at sites in New York and Connecticut revealed a high prevalence of B. microti in ticks from Suffolk County, NY...
March 2017: MSphere
https://www.readbyqxmd.com/read/28435029/activity-of-carboxylesterases-glutathione-s-transferase-and-monooxygenase-on-rhipicephalus-microplus-exposed-to-fluazuron
#10
Fabrício Nascimento Gaudêncio, Guilherme Marcondes Klafke, Vinícius Menezes Tunholi-Alves, Thaís Paes Ferreira, Cristiane Nunes Coelho, Adivaldo Henrique da Fonseca, Isabele da Costa Angelo, Jairo Pinheiro
The objective of this study was to assess the effect of the exposure to fluazuron on the activity of common pesticide detoxification enzyme groups in the cattle tick (Rhipicephalus microplus). Engorged females of a susceptible strain (POA) and a resistant strain (Jaguar) were exposed in vitro to fluazuron and their eggs and larvae were used to compare the activities of the general esterases, mixed-function oxidases (MFO) and glutathione-S-transferase (GST). The results showed significant elevation in MFO contents and esterases activity in the resistant strain when compared with the susceptible strain, in eggs and larvae respectively...
April 20, 2017: Parasitology International
https://www.readbyqxmd.com/read/28433738/crimean-congo-hemorrhagic-fever-virus-in-ticks-in-turkey-a-broad-range-tick-surveillance-study
#11
Ömer Orkun, Zafer Karaer, Ayşe Çakmak, Serpil Nalbantoğlu
In Turkey, Crimean-Congo hemorrhagic fever (CCHF) outbreaks started in northern regions in 2002. Human cases still continue to increase and the disease spreads in many other provinces of Turkey. The ecological role of the vector tick species occurring in Turkey is not certain exactly. Therefore, we planned a broad range tick study in three different ecological and geographical areas extending from the West Black Sea regions down to the Central Anatolia. The aim of this study was to determine and characterize CCHFV molecularly in feeding-ticks collected from various wild and domestic animals and from humans as well as in questing and unfed ticks...
April 19, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28433730/detection-of-rickettsia-helvetica-and-candidatus-r-tarasevichiae-dna-in-ixodes-persulcatus-ticks-collected-in-northeastern-european-russia-komi-republic
#12
Mikhail Yu Kartashov, Ludmila I Glushkova, Tamara P Mikryukova, Igor V Korabelnikov, Yulia I Egorova, Natalia L Tupota, Elena V Protopopova, Svetlana N Konovalova, Vladimir A Ternovoi, Valery B Loktev
The number of tick-borne infections in the northern European regions of Russia has increased considerably in the last years. In the present study, 676 unfed adult Ixodes persulcatus ticks were collected in the Komi Republic from 2011 to 2013 to study tick-borne rickettsioses. Rickettsia spp. DNA was detected by PCR in 51 (7.6%) ticks. The nucleotide sequence analysis of gltA fragments (765bp) from 51 ticks indicated that 60.8% and 39.2% of the ticks were infected with Rickettsia helvetica and Candidatus R. tarasevichiae, respectively...
April 6, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28433729/comparative-vertical-transmission-of-rickettsia-by-dermacentor-variabilis-and-amblyomma-maculatum
#13
Emma K Harris, Victoria I Verhoeve, Kaikhushroo H Banajee, Jacqueline A Macaluso, Abdu F Azad, Kevin R Macaluso
The geographical overlap of multiple Rickettsia and tick species coincides with the molecular detection of a variety of rickettsial agents in what may be novel tick hosts. However, little is known concerning transmissibility of rickettsial species by various tick hosts. To examine the vertical transmission potential between select tick and rickettsial species, two sympatric species of ticks, Dermacentor variabilis and Amblyomma maculatum, were exposed to five different rickettsial species, including Rickettsia rickettsii, Rickettsia parkeri, Rickettsia montanensis, Rickettsia amblyommatis, or flea-borne Rickettsia felis...
April 6, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28433728/vector-competence-of-amblyomma-americanum-acari-ixodidae-for-rickettsia-rickettsii
#14
Michael L Levin, Galina E Zemtsova, Lindsay F Killmaster, Alyssa Snellgrove, Lauren B M Schumacher
Rickettsia rickettsii - the etiologic agent of Rocky Mountain spotted fever (RMSF) - is widely spread across the Americas. In the US, Dermacentor spp. ticks are identified as primary vectors of R. rickettsii and Rhipicephalus sanguineus s.l. has been implicated in transmission of this pathogen in several locations in the Southwest. Conversely, ticks of the genus Amblyomma are recognized vectors of RMSF in Central and South America, but not in the US. A. americanum is one of the most aggressive human-biting ticks in the US, whose geographical range overlaps with that of reported RMSF cases...
April 12, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/28432926/tick-borne-encephalitis-virus-infects-human-brain-microvascular-endothelial-cells-without-compromising-blood-brain-barrier-integrity
#15
Martin Palus, Marie Vancova, Jana Sirmarova, Jana Elsterova, Jan Perner, Daniel Ruzek
Alteration of the blood-brain barrier (BBB) is a hallmark of tick-borne encephalitis (TBE), a life-threating human viral neuroinfection. However, the mechanism of BBB breakdown during TBE, as well as TBE virus (TBEV) entry into the brain is unclear. Here, primary human microvascular endothelial cells (HBMECs) were infected with TBEV to study interactions with the BBB. Although the number of infected cells was relatively low in culture (<5%), the infection was persistent with high TBEV yields (>10(6)pfu/ml)...
April 19, 2017: Virology
https://www.readbyqxmd.com/read/28432738/tick-bite-and-lyme-disease-related-emergency-department-encounters-in-new-hampshire-2010-2014
#16
E R Daly, C Fredette, A A Mathewson, K Dufault, D J Swenson, B P Chan
Lyme disease (LD) is a common tick-borne disease in New Hampshire (NH). While LD is a reportable condition and cases are counted for public health surveillance, many more people receive care for tick bites or diagnoses of LD than are reflected in surveillance data. NH's emergency department (ED) data system was queried for tick bite and LD-related encounters. Chief complaint text was queried for words related to LD or tick bites. International Classification of Diseases 9th Revision (ICD-9) codes were queried for the LD diagnosis code (088...
April 22, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28428300/draft-genome-sequence-of-enterococcus-casseliflavus-pavet15-obtained-from-the-oviduct-infection-of-the-cattle-tick-rhipicephalus-microplus-in-jiutepec-morelos-mexico
#17
R Cossío-Bayúgar, E Miranda-Miranda, C A Arreguín-Pérez, L Lozano, D Peréz de la Rosa, M K Rocha-Martínez, M A Bravo-Díaz, B Sachman-Ruiz
Enterococcus spp. are Gram-positive lactic acid-producing bacteria found in the intestinal tracts of animals, like mammals, birds, and arthropods. Enterococcus spp. may cause oportunistic infections in vertebrate and invertebrate hosts. We report here the draft genome sequence of Enterococcus casseliflavus PAVET15 containing 3,722,480 bp, with 80 contigs, an N50 of 179,476 bp, and 41.93% G+C content.
April 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28427963/molecular-investigation-of-piroplasma-infection-in-white-yaks-bos-grunniens-in-gansu-province-china
#18
Sitong Li, Junlong Liu, Aihong Liu, Youquan Li, Shuqing Wang, Shufang Wang, Hong Yin, Jianxun Luo, Guiquan Guan
Piroplasmosis, including theileriosis and babesiosis, is a tick-borne haemoprotozoan disease responsible for huge economic losses to livestock industry. In China, the biology of piroplasms infective to cattle was well understood on the basis of pathogen isolations and molecular epidemiological surveys in the past few decades. But very limited information about the infection status of piroplasms in white yak (Bos grunniens), a semi-wild and endemic breed, has been recorded, so far. A total of 350 blood samples was collected from white yaks in 11 towns of Tianzhu Tibetan Autonomous County of Gansu province, China, during April to July 2015...
April 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28427962/first-report-of-a-rickettsia-asembonensis-related-infecting-fleas-in-brazil
#19
Arannadia Barbosa Silva, Vinicius Figueiredo Vizzoni, Andréa Pereira Costa, Francisco Borges Costa, Jonas Moraes-Filho, Marcelo Bahia Labruna, Gilberto Salles Gazêta, Rita de Maria Seabra Nogueira
The present study was performed in a non-endemic area for spotted fever (SF) in Imperatriz microregion, state of Maranhão, Brazil. Blood samples and ectoparasites were collected from 300 dogs of the Imperatriz microregion. Canine serum samples were tested individually by indirect immunofluorescence assay (IFA), using five Rickettsia isolates from Brazil. Antibodies reactive to at least one of the five species of Rickettsia were detected in 1.6% of the dogs (5/300). These sera were considered reactive to Rickettsia rickettsii and Rickettsia amblyommatis or very closely related species...
April 17, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28426403/pcr-denaturing-gradient-gel-electrophoresis-as-a-useful-method-to-identify-of-intestinal-bacteria-flora-in-haemaphysalis-flava-ticks
#20
Tian-Yin Cheng, Guo-Hua Liu
Ticks are blood-sucking ectoparasites of great medical and veterinary significance that can transmit bacteria, protozoa, fungi and viruses, causing a variety of human and animal diseases worldwide. In the present study, the intestinal bacterial flora associated with Haemaphysalis flava ticks in different developmental stages were analyzed using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). Eleven distinct DGGE bands were found using PCR-DGGE method. Sequences analyses indicated that they belonged to Bacillus cereus, Candidatus rickettsia, Erwinia sp...
June 1, 2017: Acta Parasitologica
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