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autologous serum eyedrops

Antonio Pinna, Eleonora Nuvoli, Francesco Blasetti, Maria Alma Posadinu, Francesco Boscia
PURPOSE: Toxic epidermal necrolysis (TEN) is a rare, life-threatening, drug-induced, mucocutaneous disease, which can severely affect the ocular surface. The purpose of this study was to investigate the efficacy of plasmapheresis, human IV immunoglobulins (IVIg), and autologous serum (AS) eyedrops in the treatment of the severe acute ocular complications of TEN. METHODS: A retrospective chart review of all patients admitted to the Burn Unit, Azienda Ospedaliero-Universitaria-Sassari, Sassari, Italy, from 2009 to 2015, identified 9 patients (2 men, 7 women; mean age 63...
March 21, 2017: European Journal of Ophthalmology
Jesús Merayo-Lloves, Ronald M Sanchez, Ana C Riestra, Eduardo Anitua, Leire Begoña, Gorka Orive, Luis Fernandez-Vega
PURPOSE: Preliminary information about the safety and efficacy of plasma rich in growth factors (PRGF) eyedrops in the treatment of refractory cases of diverse ocular surface disorders (OSDs) is presented here. MATERIAL AND METHODS: This retrospective cohort study included cases with OSDs refractory to previous treatment with conventional treatments or autologous serum or cyclosporine, and treated with PRGF eyedrops. The signs and symptoms of ocular surface disorders [using the ocular surface disease index (OSDI), best-corrected visual acuity (BCVA), visual analog scale (VAS) frequency and VAS severity] were evaluated before and after treatment with PRGF...
2015: Ophthalmic Research
Na Hee Kang, Shina Lee, Roo Min Jun
PURPOSE: To investigate the composition and biological activity of serum epitheliotrophic factors from chronic renal failure (CRF) patients vs. healthy controls. METHODS: Twenty, 50 and 100 % autologous serum eyedrops (ASEs) were prepared from 16 CRF patients and 16 normal subjects. Serum epithelial growth factor (EGF), platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-β1 (TGF-β1) and fibronectin levels were quantified using enzyme-linked immunosorbent assay kits...
October 2015: Graefe's Archive for Clinical and Experimental Ophthalmology
José Santiago López-García, Isabel García-Lozano, Luis Rivas, Nuria Ramírez, Maria T Méndez, Rafaela Raposo
PURPOSE: The purpose of this study is to assess the stability of the growth factors (GF) in autologous serum eyedrops under different storage conditions. METHODS: The concentration of epidermal growth factor (EGF), transforming growth factor-β (TGF-β1), platelet-derived growth factor AB (PDGF-AB), and albumin was measured in fresh and defrosted samples of autologous serum under different storage conditions. The fresh and defrosted samples were cooled at 4 °C, and they were studied immediately after preparation, or after defrosting, and after 1, 2, 3, and 4 weeks...
2016: Current Eye Research
Deniz Hos, Konrad R Koch, Franziska Bucher, Felix Bock, Claus Cursiefen, Ludwig M Heindl
PURPOSE: The effect of autologous serum eyedrops on the corneal vasculature is undefined. Therefore, we analyzed the corneal vascular effects of serum eyedrops in comparison with and in combination with bevacizumab eyedrops. METHODS: In vitro, blood and lymphatic endothelial cells were treated with serum eyedrops, bevacizumab eyedrops, or a combination of both, and cell proliferation was measured. In vivo, inflammatory corneal neovascularization was induced by suture placement...
2013: Investigative Ophthalmology & Visual Science
Empar Sanz-Marco, Patricia Udaondo, Salvador García-Delpech, Amparo Vazquez, Manuel Diaz-Llopis
PURPOSE: We investigate the efficacy of 0.03% topical tacrolimus eyedrops for the treatment of dry eye in graft versus host disease (GVHD) patients resistant/intolerant to 0.05% topical cyclosporine. METHODS: Forty-three patients were enrolled in this prospective study. After completing a 1-year run-in period of using artificial tears, 50% autologous serum eyedrops, and punctal plug occlusion, all the symptomatic patients (n=29) were treated with 0.05% topical cyclosporine (Restasis(®); Allergan, Inc...
October 2013: Journal of Ocular Pharmacology and Therapeutics
B Seitz, M El-Husseiny, A Langenbucher, N Szentmáry
Besides routine postoperative follow-up the prophylaxis of complications in penetrating keratoplasty (PKP) includes special preoperative and intraoperative aspects. Preoperative prophylaxis consists of the therapy of systemic diseases and eyelid abnormalities, determining individual optimal graft size, avoiding PKP in cases of uncontrolled intraocular pressure, avoiding PKP in cases of corneal hydrops, pretreatment of vascularized cornea, amniotic membrane transplantation before PKP in cases of ulcerative keratitis, quality controlled organ-cultured transplants and preoperative counselling by the surgeon to ensure patient compliance...
July 2013: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
W Ghouali, O Sandali, B Ameline, E Basli, I Goemaere, V Borderie, L Laroche
A 48-year-old woman with no significant past history underwent bilateral simultaneous laser in situ keratomileusis for correction of her myopia. On the tenth postoperative day, the patient complained of visual decrease and photophobia. Slit lamp exam showed corneal epithelial irregularities. Confocal microscopy was performed and revealed a characteristic appearance of epithelial basement membrane dystrophy (EBMD). The patient was successfully treated with artificial tears and autologous serum eyedrops. EBMD may be missed before LASIK surgery, even after a careful pre-operative examination...
October 2013: Journal Français D'ophtalmologie
Kyoung Min Kim, Yong-Tae Shin, Hong Kyun Kim
PURPOSE: Platelet-rich plasma (PRP) harbors high concentrations of growth factors related to the promotion of wound healing. We evaluated the efficacy of PRP eyedrops in the treatment of persistent epithelial defects (PEDs). METHODS: Autologous PRP and autologous serum (AS) were prepared from whole blood. The concentrations of transforming growth factor (TGF)-β1, TGF-β2, epidermal growth factor (EGF), vitamin A and fibronectin in the PRP and AS were analyzed and compared...
November 2012: Japanese Journal of Ophthalmology
José S López-García, Isabel García-Lozano
OBJECTIVE: To assess the effect of the use of containers with an adapted sterilizing filter on the contamination of autologous serum eyedrops. DESIGN: Prospective, consecutive, comparative, and randomized study. PARTICIPANTS: Thirty patients with Sjögren syndrome. METHODS: One hundred seventy-six autologous serum containers used in home therapy were studied; 48 of them included an adapted filter (Hyabak; Thea, Clermont-Ferrand, France), and the other 128 were conventional containers...
November 2012: Ophthalmology
Asimina Mataftsi, Areti Bourtoulamaiou, Konstantinos Anastasilakis, Nikolaos G Ziakas, Stavros A Dimitrakos
PURPOSE: The aim of this study was to report the use of autologous serum eyedrops (ASEs) for resolution of a corneal ulcer secondary to bullous keratopathy. METHODS: This is a case report. RESULTS: A 66-year-old patient presented with an infected ulcer and hypopyon while using a bandage contact lens for bullous keratopathy. Staphylococcus warneri infection was treated with systemic and topical antibiotics, and ASEs were subsequently administered to enhance reepithelialization and to avoid the need for a bandage contact lens...
July 2013: Eye & Contact Lens
Cristhian A Urzua, Dario H Vasquez, Andres Huidobro, Helio Hernandez, Jorge Alfaro
PURPOSE: To determine symptoms improvement in dry eye patients with short-term autologous serum (AS) eyedrops treatment using the standardized Ocular Surface Disease Index (OSDI) survey. MATERIALS AND METHODS: A double-blind randomized crossover clinical trial was conducted, comparing short-term (2 weeks) topical treatment with AS eyedrops diluted at 20% versus conventional artificial tears treatment in adult severe dry eye syndrome (DES) patients. The main outcome measure was assessment of symptoms with OSDI survey...
August 2012: Current Eye Research
Jorge L Alio, Francisco Arnalich-Montiel, Alejandra E Rodriguez
Blood derived products have demonstrated their capacity to enhance healing and stimulate the regeneration of different tissues and this enhancing effect is attributed to the growth factors and bioactive proteins that are synthesized and present in blood. Eye platelet rich plasma (E-PRP) provides higher concentration of essential growth factors and cell adhesion molecules by concentrating platelets in a small volume of plasma as compared with autologous serum, the latter being used widely in ophthalmology for epithelial wound healing of the cornea for the last two decades...
June 2012: Current Pharmaceutical Biotechnology
Michał Wilczyński
In the literature, there is evidence suggesting that autologous serum eyedrops can be helpful in the treating various ocular conditions. Using eyedrops containing the 20-100% solution of patient's own serum, centrifuged 3000-4000 rpm, diluted in either saline, BSS or chloramphenicol solution, is a relatively new therapeutic strategy. Nevertheless, in some publications, a positive influence of using autologous serum topically has been proven in numerous ocular diseases. It has been found, that using autologous serum eyedrops is safe, such eyedrops can be stored in temperature ranging from -30 degrees C to +4 degrees C...
2009: Klinika Oczna
Bennie H Jeng, William J Dupps
PURPOSE: To evaluate the success rate of treating persistent corneal epithelial defects using 50% autologous serum eyedrops. METHODS: We reviewed the medical records of all patients seen at our institution from September 2004 to May 2007 who had persistent corneal epithelial defects that were nonresponsive to conventional medical treatment and that were treated with 50% autologous serum eyedrops. We also correlated the defect duration prior to initiation of serum therapy with rate of epithelial healing...
December 2009: Cornea
H A Shahriari, Famil Tokhmehchi, Mohammad Reza, N F Hashemi
PURPOSE: Alkaline corneal epithelial wound closure rates in rabbit eyes were compared after topical applications of amniotic membrane suspension, autologous serum, or preservative-free artificial tears. METHODS: Thirty rabbits were divided into 3 randomization groups of 10 rabbits each. Central corneal alkali wound was produced in both eyes of the rabbits by applying a 5-mm round filter paper, soaked in 1 N NaOH, for 60 seconds. Group 1 animals received preservative-free artificial tears in 1 eye and amniotic membrane suspension in the other eye; group 2 animals received preservative-free artificial tears in 1 eye and autologous serum in the other eye; and group 3 animals received autologous serum in 1 eye and amniotic membrane suspension in the other eye...
December 2008: Cornea
K Kasper, L Godenschweger, D Hartwig, J D Unterlauft, B Seitz, G Geerling
PURPOSE: The use of serum eye drops has become more common for treating ocular surface diseases such as persistent epithelial defect and dry eye. For the production and use of blood products, regulatory restrictions apply. We surveyed the practice of production and application of serum eyedrops among the members of the Cornea Section of the German Ophthalmological Society (DOG) in Germany. METHODS: A questionnaire concerning the application of topical blood-derived therapeutic agents for use in the eye was sent to 103 institutions in 2005 and 2007...
July 2008: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
S Maclennan, D Hartwig, G Geerling
The majority of patients with dry eye syndromes respond to conventional treatment aimed at optimising the ocular surface environment. There are some, however, who do not respond adequately to conventional lubricants. The first description of the use of autologous serum as a nutrient tears substitute was published more than 20 years ago. In 1997, NHS Blood and Transplant (NHSBT) developed a reliable and reproducible method for the production of eyedrops derived from autologous serum according to GMP Guidelines...
July 2008: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
Teruo Nishida
The physiological roles of the cornea are to conduct external light into the eye, focus it, together with the lens, onto the retina, and to provide rigidity to the entire eyeball. Good vision thus requires maintenance of the transparency and proper refractive shape of the cornea. Although the cornea appears to be a relatively static structure, dynamic processes operate within and around the cornea at the tissue, cell, and molecular level. In this article, I review the mechanisms responsible for maintenance of corneal homeostasis as well as the development of new modes of treatment for various corneal diseases...
March 2008: Nippon Ganka Gakkai Zasshi
Chisa Yamada, Karen E King, Paul M Ness
Persistent corneal epithelial defects (CEDs) are caused by many diseases that are usually associated with decreased production of tears or reduced corneal sensitivity. Although surgical treatments are available for severe cases, CEDs are still difficult for ophthalmologists to treat. One treatment for CEDs that is uncommonly used in the United States is autologous serum eyedrops (ASEs). The first application of ASEs was described in 1984. This landmark report was followed by multiple studies that carefully evaluated the epithelial-promoting properties of ASEs and refined the means of ASE preparation...
June 2008: Transfusion
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