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https://www.readbyqxmd.com/read/27917279/minocycline-attenuates-cirrhotic-cardiomyopathy-and-portal-hypertension-in-a-rat-model-possible-involvement-of-nitric-oxide-pathway
#1
Seyyedeh Elaheh Mousavi, Seyed Mahdi Rezayat, Maliheh Nobakht, Seyed Soheil Saeedi Saravi, Iraj Yazdani, Amir Rashidian, Ahmad Reza Dehpour
OBJECTIVES: An increase in nitric oxide (NO) production has been reported in cirrhotic cardiomyopathy and, portal hypertension. Since minocycline has been shown to inhibit NO overproduction, we aimed to examine its role in a rat model of CCl4-induced cirrhotic cardiovascular complications. MATERIALS AND METHODS: Portal pressure and inotropic responsiveness of isolated papillary muscles to isoproterenol were measured in cirrhotic rats, following minocycline (50 mg/kg/day for 8 weeks) treatment...
November 2016: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/27824007/tetrahydrobiopterin-protects-against-radiation-induced-growth-inhibition-in-h9c2-cardiomyocytes
#2
Zheng-Yi Zhang, Yi Li, Rui Li, An-An Zhang, Bo Shang, Jing Yu, Xiao-Dong Xie
BACKGROUND: Tetrahydrobiopterin (BH4) is an essential cofactor of nitric oxide synthases (NOSs) for the synthesis of nitric oxide (NO). BH4 therapy can reverse the disease-related redox disequilibrium observed with BH4 deficiency. However, whether BH4 exerts a protective effect against radiation-induced damage to cardiomyocytes remains unknown. METHODS: Clonogenic assays were performed to determine the effects of X-ray on H9c2 cells with or without BH4 treatment...
2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27795807/cox-2-inhibition-protects-against-hypoxia-reoxygenation-induced-cardiomyocyte-apoptosis-via-akt-dependent-enhancement-of-inos-expression
#3
Lei Pang, Yin Cai, Eva Hoi Ching Tang, Dan Yan, Ramoji Kosuru, Haobo Li, Michael G Irwin, Haichun Ma, Zhengyuan Xia
The present study explored the potential causal link between ischemia-driven cyclooxygenase-2 (COX-2) expression and enhanced apoptosis during myocardial ischemia/reperfusion (I/R) by using H9C2 cardiomyocytes and primary rat cardiomyocytes subjected to hypoxia/reoxygenation (H/R). The results showed that H/R resulted in higher COX-2 expression than that of controls, which was prevented by pretreatment with Helenalin (NFκB specific inhibitor). Furthermore, pretreatment with NS398 (COX-2 specific inhibitor) significantly attenuated H/R-induced cell injury [lower lactate dehydrogenase (LDH) leakage and enhanced cell viability] and apoptosis (higher Bcl2 expression and lower level of cleaved caspases-3 and TUNEL-positive cells) in cardiomyocytes...
2016: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/27792175/the-relationship-between-estrogen-and-nitric-oxide-in-the-prevention-of-cardiac-and-vascular-anomalies-in-the-developing-zebrafish-danio-rerio
#4
Benjamin G Sykes, Peter M Van Steyn, Jonathan D Vignali, John Winalski, Julie Lozier, Wade E Bell, James E Turner
It has been known that both estrogen (E2) and nitric oxide (NO) are critical for proper cardiovascular system (CVS) function. It has also been demonstrated that E2 acts as an upstream effector in the nitric oxide (NO) pathway. Results from this study indicate that the use of a nitric oxide synthase (NOS) inhibitor (NOSI) which targets specifically neuronal NOS (nNOS or NOS1), proadifen hydrochloride, caused a significant depression of fish heart rates (HR) accompanied by increased arrhythmic behavior. However, none of these phenotypes were evident with either the inhibition of endothelial NOS (eNOS) or inducible NOS (iNOS) isoforms...
October 26, 2016: Brain Sciences
https://www.readbyqxmd.com/read/27665951/inhibition-of-inducible-nitric-oxide-synthase-inos-by-simvastatin-attenuates-cardiac-hypertrophy-in-rats
#5
Aly Mohamed Ahmed
The left ventricular hypertrophy (LVH) occurs in response to the hemodynamic overload in some physiological and pathological conditions. This study was designed to investigate the possible cardioprotective effect of simvastatin (SIM) treatment against isoproterenol (ISO)-induced LVH and the probable underlying mechanism in adult male Wistar rats. Animals were allocated into 4 groups. Rats of control group received normal saline orally for 30 days and intraperitoneally for the last 7 days. Rats of SIM group received SIM orally (10 mg/kg/day in saline) for 30 days...
September 26, 2016: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/27607345/protective-role-of-gper-agonist-g-1-on-cardiotoxicity-induced-by-doxorubicin
#6
Ernestina M De Francesco, Carmine Rocca, Francesco Scavello, Daniela Amelio, Teresa Pasqua, Damiano C Rigiracciolo, Andrea Scarpelli, Silvia Avino, Francesca Cirillo, Nicola Amodio, Maria C Cerra, Marcello Maggiolini, Tommaso Angelone
The use of Doxorubicin (Dox), a frontline drug for many cancers, is often complicated by dose-limiting cardiotoxicity in approximately 20% of patients. The G-protein estrogen receptor GPER/GPR30 mediates estrogen action as the cardioprotection under certain stressful conditions. For instance, GPER activation by the selective agonist G-1 reduced myocardial inflammation, improved immunosuppression, triggered pro-survival signaling cascades, improved myocardial mechanical performance and reduced infarct size after ischemia/reperfusion (I/R) injury...
September 8, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27566322/interleukin-17a-mediates-cardiomyocyte-apoptosis-through-stat3-inos-pathway
#7
Sheng-An Su, Du Yang, Wei Zhu, Zhejun Cai, Na Zhang, Lina Zhao, Jian-An Wang, Meixiang Xiang
Interleukin-17A, a pro-inflammatory cytokine, has a direct proapoptotic effect on cardiomyocytes. However, the specific mechanism has not been clarified. In the present study, an in-vitro model of cardiomyocyte apoptosis induced by IL-17A stimulation was employed and the roles of iNOS and Stat3 involved were investigated. Our data showed that the neonatal mouse cardiomyocytes express IL-17 receptors: IL-17RA and IL-17RC, but did not express IL-17A. Exogenous IL-17A significantly induces iNOS expression and hence the cardiomyocyte apoptosis...
November 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27447611/argon-induces-protective-effects-in-cardiomyocytes-during-the-second-window-of-preconditioning
#8
Britta Mayer, Josefin Soppert, Sandra Kraemer, Sabrina Schemmel, Christian Beckers, Christian Bleilevens, Rolf Rossaint, Mark Coburn, Andreas Goetzenich, Christian Stoppe
Increasing evidence indicates that argon has organoprotective properties. So far, the underlying mechanisms remain poorly understood. Therefore, we investigated the effect of argon preconditioning in cardiomyocytes within the first and second window of preconditioning. Primary isolated cardiomyocytes from neonatal rats were subjected to 50% argon for 1 h, and subsequently exposed to a sublethal dosage of hypoxia (<1% O₂) for 5 h either within the first (0-3 h) or second window (24-48 h) of preconditioning...
2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27284355/sitagliptin-attenuates-in%C3%AF-ammatory-responses-in-lipopolysaccharide-stimulated-cardiomyocytes-via-nuclear-factor-%C3%AE%C2%BAb-pathway-inhibition
#9
Chien-Hung Lin, Chung-Ching Lin
Glucagon-like peptide-1 (GLP-1) and GLP-1 receptors (GLP-1Rs) are responsible for glucose homeostasis, and have been shown to reduce inflammation in preclinical studies. The aim of the present study was to determine whether sitagliptin, an inhibitor of the enzyme dipeptidyl peptidase-4 (DPP-4), as a GLP-1 receptor agonist, exerts an anti-inflammatory effect on cardiomyoblasts during lipopolysaccharide (LPS) stimulation. Exposure to LPS increased the expression levels of tumor necrosis factor (TNF)-α, interleukin-6 (IL)-6 and IL-1β in H9c2 cells, and also resulted in elevations in cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression and nuclear factor-κB (NF-κB) nuclear translocation...
June 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27222135/association-of-cardiac-injury-with-iron-increased-oxidative-and-nitrative-modifications-of-the-serca2a-isoform-of-sarcoplasmic-reticulum-ca-2-atpase-in-diabetic-rats
#10
Xueli Li, Wenliang Li, Zhonghong Gao, Hailing Li
The role of iron in the etiology of diabetes complications is not well established. Thus, this study was performed to test whether the iron-induced increase of oxidative/nitrative damage is involved in SERCA2a-related diabetic heart complication. Four randomly divided groups of rats were used: normal control group; iron overload group; diabetes group, and diabetic plus iron overload group. Iron supplementation stimulated cardiomyocyte hypertrophy and led to an increase in cardiac protein carbonyls, nitrotyrosine (3-NT) formation, and iNOS protein expression, thus resulting in abnormal myocardium calcium homeostasis of diabetic rats...
August 2016: Biochimie
https://www.readbyqxmd.com/read/27027354/poly-adp-ribose-polymerase-1-inhibition-protects-cardiomyocytes-from-inflammation-and-apoptosis-in-diabetic-cardiomyopathy
#11
Wei-Dong Qin, Guo-Liang Liu, Juan Wang, Hao Wang, Jian-Ning Zhang, Fan Zhang, Yang Ma, Xin-Ying Ji, Chen Li, Ming-Xiang Zhang
Diabetic cardiomyopathy (DCM) is characterized by structural alterations such as cardiomyocyte hypertrophy, necrosis and focal fibrosis. Poly(ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme which can be activated by DNA damage and plays a critical role in various diseases. We hypothesized that PARP-1 may play an important role in DCM and that its inhibition may protect cardiomyocytes from inflammation and apoptosis in DCM. H9c2 cardiomyocytes were treated with normal glucose, mannitol or high glucose (HG)...
June 14, 2016: Oncotarget
https://www.readbyqxmd.com/read/26873862/a-new-in-vitro-co-culture-model-using-magnetic-force-based-nanotechnology
#12
Hiroki Takanari, Keiko Miwa, XianMing Fu, Junichi Nakai, Akira Ito, Kousuke Ino, Hiroyuki Honda, Wataru Tonomura, Satoshi Konishi, Tobias Opthof, Marcel Ag van der Heyden, Itsuo Kodama, Jong-Kook Lee
Skeletal myoblast (SkMB) transplantation has been conducted as a therapeutic strategy for severe heart failure. However, arrhythmogenicity following transplantation remains unsolved. We developed an in vitro model of myoblast transplantation with "patterned" or "randomly-mixed" co-culture of SkMBs and cardiomyocytes enabling subsequent electrophysiological, and arrhythmogenic evaluation. SkMBs were magnetically labeled with magnetite nanoparticles and co-cultured with neonatal rat ventricular myocytes (NRVMs) on multi-electrode arrays...
October 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/26762089/-octarphin-nonopioid-peptide-of-the-opioid-origin
#13
REVIEW
E V Navolotskaya
The data on the properties and mechanism of action of the peptide octarphin (TPLVTLFK, the fragment 12-19 of β-endorphin)--a selective agonist of nonopioid (insensitive to the action of the opioid antagonist naloxone) β-endorphin receptor found on n immune cells (peritoneal macrophages, T and B lymphocytes of spleen and blood), endocrine (adrenal cortex, hypothalamus), cardiovascular (cardiomyocytes) systems are analyzed and systematized. Binding to the receptor octarphin increases increases the mitogen-induced pro- liferation of human and mouse T and B lymphocytes in vitro, activates murine peritoneal macrophages in vitro and in vivo, stimulates growth of human T-lymphoblast cell lines Jurkat and MT-4, inhibits adenylate cyclase activity of rat adrenal cortex membranes and suppresses the secretion of glucocorticoids from the adrenal gland into the blood...
September 2015: Bioorganicheskaia Khimiia
https://www.readbyqxmd.com/read/26681542/overexpression-myocardial-inducible-nitric-oxide-synthase-exacerbates-cardiac-dysfunction-and-beta-adrenergic-desensitization-in-experimental-hypothyroidism
#14
Qun Shao, Heng-Jie Cheng, Michael F Callahan, Dalane W Kitzman, Wei-Min Li, Che Ping Cheng
BACKGROUND: Altered nitric oxide synthase (NOS) has been implicated in the pathophysiology of heart failure (HF). Recent evidence links hypothyroidism to the pathology of HF. However, the precise mechanisms are incompletely understood. The alterations and functional effects of cardiac NOS in hypothyroidism are unknown. We tested the hypothesis that hypothyroidism increases cardiomyocyte inducible NOS (iNOS) expression, which plays an important role in hypothyroidism-induced depression of cardiomyocyte contractile properties, [Ca(2+)]i transient ([Ca(2+)]iT), and β-adrenergic hyporesponsiveness...
February 1, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/26553043/topological-control-of-nitric-oxide-secretion-by-tantalum-oxide-nanodot-arrays
#15
Udesh Dhawan, Chia Hui Lee, Chun-Chung Huang, Ying Hao Chu, Guewha S Huang, Yan-Ren Lin, Wen-Liang Chen
BACKGROUND: Nitric oxide (NO) plays a very important role in the cardiovascular system as a major secondary messenger in signaling pathway. Its concentration regulates most of the important physiological indexes including the systemic blood pressure, blood flow, regional vascular tone and other cardiac functions. The effect of nanotopography on the NO secretion in cardiomyocytes has not been elucidated before. In this study, we report how the nanotopography can modulate the secretion profile of NO and attempt to elucidate the genetic pathways responsible for the same by using Tantalum Oxide nanodot arrays ranging from 10 to 200 nm...
2015: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/26528797/levosimendan-inhibits-interleukin-1%C3%AE-induced-apoptosis-through-activation-of-akt-and-inhibition-of-inducible-nitric-oxide-synthase-in-rat-cardiac-fibroblasts
#16
Muneyoshi Okada, Hideyuki Yamawaki
Levosimendan, a calcium sensitizer, known as an inotropic agent has a cytoprotective effect against apoptosis in cardiomyocytes. However, the cytoprotective effect of levosimendan on cardiac fibroblasts has not been clarified. The aim of this study was to examine whether levosimendan modulates interleukin (IL)-1β-induced apoptosis in adult rat cardiac fibroblast. Cardiac fibroblasts were isolated from adult male Wistar rats. Apoptosis of cardiac fibroblasts was evaluated by 4',6-diamidino-2-phenylindole staining and TdT-mediated d-UTP nick end labeling (TUNEL)-based staining...
December 15, 2015: European Journal of Pharmacology
https://www.readbyqxmd.com/read/26488377/therapeutic-effects-of-pentoxifylline-on-diabetic-heart-tissue-via-nos
#17
Derya Karabulut, Hasan Basri Ulusoy, Emin Kaymak, Mehmet Fatih Sönmez
OBJECTIVE: Diabetes mellitus causes a decrease in cardiac output, arterial blood pressure, and heart rate. In this study, we aimed to investigate, at the molecular level, the effect of nitric oxide synthase (NOS) on heart pathology in type 1 diabetes and look at the therapeutic effect of pentoxifylline on this pathology. METHODS: In this experimental study, 50 Wistar albino male rats were used. The rats were divided into 5 groups: group C, control; group D, only diabetes; group D+PI and D+PII, diabetes + pentoxifylline; group P, only pentoxifylline...
May 2016: Anatolian Journal of Cardiology
https://www.readbyqxmd.com/read/26362204/increased-oxidative-stress-contributes-to-cardiomyocyte-dysfunction-and-death-in-patients-with-fabry-disease-cardiomyopathy
#18
Cristina Chimenti, Fernanda Scopelliti, Elisabetta Vulpis, Marco Tafani, Lidia Villanova, Romina Verardo, Ruggero De Paulis, Matteo A Russo, Andrea Frustaci
Cardiac dysfunction of Fabry disease (FD) has been associated with myofilament damage and cell death as result of α-galactosidase A deficiency and globotriaosylceramide accumulation. We sought to evaluate the role of oxidative stress in FD cardiomyocyte dysfunction. Myocardial tissue from 18 patients with FD was investigated for the expression of inducible nitric oxide synthase (iNOS) and nitrotyrosine by immunohistochemistry. Western blot analysis for nitrotyrosine was also performed. Oxidative damage to DNA was investigated by immunostaining for 8-hydroxydeoxyguanosine (8-OHdG), whereas apoptosis was evaluated by in situ ligation with hairpin probes...
November 2015: Human Pathology
https://www.readbyqxmd.com/read/26361649/inducible-no-synthase-is-constitutively-expressed-in-porcine-myocardium-and-its-level-decreases-along-with-tachycardia-induced-heart-failure
#19
Urszula Paslawska, Liliana Kiczak, Jacek Bania, Robert Paslawski, Adrian Janiszewski, Piotr Dzięgiel, Maciej Zacharski, Alicja Tomaszek, Katarzyna Michlik
BACKGROUND: The adverse effects of oxidative stress and the presence of proinflammatory factors in the heart have been widely demonstrated mainly on rodent models. However, larger clinical trials focusing on inflammation or oxidative stress in heart failure (HF) have not been carried out. This may be due to differences in the anatomy and physiology of the cardiovascular system between small rodents and large mammals. Thus, we investigated myocardial inflammatory factors, such as inducible NO synthase (iNOS) and oxidative stress indices in female pigs with chronic tachycardia-induced cardiomyopathy...
January 2016: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/26334033/phosphoinositide-3-kinase-gamma-controls-inflammation-induced-myocardial-depression-via-sequential-camp-and-inos-signalling
#20
Bernadin Ndongson-Dongmo, Regine Heller, Dirk Hoyer, Michael Brodhun, Michael Bauer, Johannes Winning, Emilio Hirsch, Reinhard Wetzker, Peter Schlattmann, Reinhard Bauer
AIMS: Sepsis-induced myocardial depression (SIMD), an early and frequent event of infection-induced systemic inflammatory response syndrome (SIRS), is characterized by reduced contractility irrespective of enhanced adrenergic stimulation. Phosphoinositide-3 kinase γ (PI3Kγ) is known to prevent β-adrenergic overstimulation via its scaffold function by activating major cardiac phosphodiesterases and restricting cAMP levels. However, the role of PI3Kγ in SIRS-induced myocardial depression is unknown...
November 1, 2015: Cardiovascular Research
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