María Garranzo-Asensio, Ana Guzmán-Aránguez, Carmen Povés, María Jesús Fernández-Aceñero, Ana Montero-Calle, María Ángeles Ceron, Servando Fernandez-Diez, Nuria Rodríguez, Marta Gómez de Cedrón, Ana Ramírez de Molina, Gemma Domínguez, Rodrigo Barderas
The p53-family is tightly regulated at transcriptional level. Due to alternative splicing, up to 40 different theoretical proteoforms have been described for p73 and at least 20 and 10 for p53 and p63, respectively. However, only the canonical proteins have been evaluated as autoantibody targets in cancer patients for diagnosis. In this study, we have cloned and expressed in vitro the most upregulated proteoforms of p73, ΔNp73α and ΔNp73β, for the analysis of their seroreactivity by a developed luminescence based immunoassay test using 145 individual plasma from colorectal cancer, premalignant individuals and healthy controls...
September 19, 2019: Scientific Reports