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https://www.readbyqxmd.com/read/29914975/simultaneous-activation-of-innate-and-adaptive-immunity-participates-in-the-development-of-renal-injury-in-a-model-of-heavy-proteinuria
#1
Viviane Dias Faustino, Simone Costa Alarcon Arias, Victor Ferreira Ávila, Orestes Foresto-Neto, Fernanda Florencia Fregnan Zambom, Flavia Gomes Machado, Luciene Machado Dos Reis, Denise Maria Avancini Costa Malheiros, Rildo Aparecido Volpini, Niels Olsen Saraiva Camara, Roberto Zatz, Clarice Kazue Fujihara
Protein overload of proximal tubular cells can promote interstitial injury by unclear mechanisms that may involve activation of innate immunity. We investigated whether prolonged exposure of tubular cells to high protein concentrations stimulates innate immunity, triggering progressive interstitial inflammation and renal injury, and whether specific inhibition of innate or adaptive immunity would provide renoprotection in an established model of massive proteinuria, adriamycin (ADR) nephropathy. Adult male Munich-Wistar rats received a single dose of ADR (5 mg/kg iv), being followed for 2, 4 or 20 weeks...
June 18, 2018: Bioscience Reports
https://www.readbyqxmd.com/read/29908751/induction-of-uterine-hyperplasia-after-cafeteria-diet-exposure
#2
María Paula Gastiazoro, Marlise Guerrero Schimpf, Milena Durando, Gisela Paola Lazzarino, María Florencia Andreoli, Oliver Zierau, Enrique Hugo Luque, Jorge Guillermo Ramos, Jorgelina Varayoud
Our aim was to evaluate whether chronic administration of CAF affects the uterus and induces the morphological and molecular changes associated with endometrial hyperplasia. Female Wistar rats exposed to CAF from weaning for 20 weeks displayed increased energy intake, body weight and fat depots, but did not develop metabolic syndrome. The adult uteri showed an increase in glandular volume fraction and stromal area. The epithelial proliferation rate and protein expression of oestrogen receptor alpha (ERα) were also increased...
June 14, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29898726/development-of-a-production-chain-from-vegetable-biowaste-to-platform-chemicals
#3
Annemarie Schmidt, Gunnar Sturm, Christian Jonas Lapp, Daniel Siebert, Florencia Saravia, Harald Horn, Padma Priya Ravi, Andreas Lemmer, Johannes Gescher
BACKGROUND: A future bioeconomy relies on the development of technologies to convert waste into valuable compounds. We present here an attempt to design a biotechnological cascade for the conversion of vegetable waste into acetoin and electrical energy. RESULTS: A vegetable waste dark fermentation effluent containing mainly acetate, butyrate and propionate was oxidized in a bioelectrochemical system. The achieved average current at a constant anode potential of 0 mV against standard hydrogen electrode was 177...
June 13, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29895412/benefit-of-an-electronic-medical-record-based-alarm-in-the-optimization-of-stress-ulcer-prophylaxis
#4
Emanuel José Saad, Marianela Bedini, Ana Florencia Becerra, Gustavo Daniel Martini, Jacqueline Griselda Gonzalez, Andrea Bolomo, Luciana Castellani, Silvana Quiroga, Cristian Morales, James Leathers, Domingo Balderramo, Ricardo Arturo Albertini
BACKGROUND: The use of stress ulcer prophylaxis (SUP) has risen in recent years, even in patients without a clear indication for therapy. AIM: To evaluate the efficacy of an electronic medical record (EMR)-based alarm to improve appropriate SUP use in hospitalized patients. METHODS: We conducted an uncontrolled before-after study comparing SUP prescription in intensive care unit (ICU) patients and non-ICU patients, before and after the implementation of an EMR-based alarm that provided the correct indications for SUP...
June 9, 2018: Gastroenterología y Hepatología
https://www.readbyqxmd.com/read/29893585/false-positive-phencyclidine-immunoassay-results-caused-by-metronidazole
#5
Nicolás Fernández, Florencia Falguera, Laura M Cabanillas, Patricia N Quiroga
No abstract text is available yet for this article.
June 12, 2018: Clinical Toxicology
https://www.readbyqxmd.com/read/29893205/effective-population-size-differences-in-calomys-musculinus-the-host-of-jun%C3%A3-n-virus-its-relationship-with-the-epidemiological-history-of-argentine-hemorrhagic-fever
#6
Marina B Chiappero, María Florencia Piacenza, María Cecilia Provensal, Gladys E Calderón, Cristina N Gardenal, Jaime J Polop
Argentine hemorrhagic fever (AHF) is a serious endemic disease in Argentina, produced by Junín virus, whose host is the Sigmodontinae rodent Calomys musculinus . Within the endemic area, human incidence and proportion of infected rodents remains high for 5-10 years after the first appearance of the disease (epidemic [E] zone) and then gradually declines to sporadic cases (historic [H] zone). We tested the hypothesis that host populations within the E zone are large and well connected by gene flow, facilitating the transmission and maintenance of the virus, whereas those in the H and nonendemic (NE) zones are small and isolated, with the opposite effect...
June 11, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29891608/molecular-confirmation-of-the-linkage-between-rhizopus-oryzae-cyp51a-gene-coding-region-and-its-intrinsic-voriconazole-and-fluconazole-resistance
#7
Daiana Macedo, Florencia Leonardelli, Catiana Dudiuk, Laura Theill, Matías S Cabeza, Soledad Gamarra, Guillermo Garcia-Effron
Rhizopus oryzae is the most prevalent causative agent of Mucormycosis, an increasingly reported opportunistic fungal infection. These mucormycetes are intrinsically resistant to Candida - and Aspergillus -active antifungal azole drugs such as fluconazole and voriconazole, respectively. Despite of its importance, the molecular mechanisms of its intrinsic azole resistance have not been elucidated yet. The aim of this work is to establish if the Rhizopus oryzae CYP51 genes are the unique responsible for voriconazole and fluconazole intrinsic resistance in these fungal pathogens...
June 11, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29888224/management-of-field-evolved-resistance-to-bt-maize-in-argentina-a-multi-institutional-approach
#8
Ana M Signorini, Gustavo Abratti, Damián Grimi, Marcos Machado, Florencia F Bunge, Betiana Parody, Laura Ramos, Pablo Cortese, Facundo Vesprini, Agustina Whelan, Mónica P Araujo, Mariano Podworny, Alejandro Cadile, María F Malacarne
Evolution of resistance to control measures in insect populations is a natural process, and management practices are intended to delay or mitigate resistance when it occurs. During the 2012/13 season the first reports of unexpected damage by Diatraea saccharalis on some Bt maize hybrids occurred in the northeast of San Luis province, Argentina. The affected Bt technologies were Herculex I® (HX-TC1507) and VT3PRO® (MON 89034 × MON 88017* ). Event TC1507 expresses Cry1F and event MON 89034 expresses Cry1A...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29885233/competition-does-not-come-at-the-expense-of-colonization-in-seed-morphs-with-increased-size-and-dispersal
#9
M Florencia Miguel, Christopher J Lortie, Ragan M Callaway, José L Hierro
PREMISE OF THE STUDY: Seed-level trade-offs of heterocarpic species remain poorly understood. We propose that seedlings emerging from seeds with a permanent pappus (dispersing seeds) are stronger competitors than those emerging from seeds without a pappus (nondispersing seeds) because dispersing seeds are larger and germinate faster than nondispersing seeds in Centaurea solstitialis. METHODS: We conducted a competition experiment with both seed morphs, in which we recorded emergence rate and proportion, estimated seed dispersal by wind (anemochory) and by mammals (exozoochory), and measured size and abundance of seed morphs...
September 2017: American Journal of Botany
https://www.readbyqxmd.com/read/29885006/equine-chorionic-gonadotropin-ecg-improves-bucks-semen-quality-during-the-nonbreeding-season
#10
Florencia Beracochea, María Noel Viera, Lucía Acevedo, Julián Santiago-Moreno, Rodolfo Ungerfeld
In most goat breeds, testosterone serum concentration and semen quality decrease during the nonbreeding season. However, bucks reproductive activity may be stimulated with the administration of equine chorionic gonadotropin (eCG). Therefore, the aim of this study was to determine whether the repeated administration of eCG stimulates the reproductive status of bucks during the nonbreeding season. The study was performed with 19 bucks that were assigned to a group that was treated with eCG (GeCG) and an untreated control group (GCon)...
June 8, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29884399/optimization-of-%C3%AE-cyclodextrin-based-extraction-of-antioxidant-and-anti-browning-activities-from-thyme-leaves-by-response-surface-methodology
#11
Leonardo Cristian Favre, Cristina Dos Santos, María Paula López-Fernández, María Florencia Mazzobre, María Del Pilar Buera
Thyme (Thymus vulgaris) has been demonstrated to extend the shelf-life of food products, being also a potential source of bioactive compounds. The aim of this research was to optimize the ultrasound assisted extraction employing β-cyclodextrin aqueous solutions as no-contaminant technology and Response Surface Methodology to obtain thyme extracts with the maximum antioxidant capacity. The optimal extraction conditions were: a solution of β-ciclodextrin 15 mM, an ultrasonic treatment time of 5.9 min at a temperature of 36...
November 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29882614/drivers-of-international-variation-in-prevalence-of-disabling-low-back-pain-findings-from-the-cupid-study
#12
David Coggon, Georgia Ntani, Keith T Palmer, Vanda E Felli, Florencia Harari, Leonardo A Quintana, Sarah A Felknor, Marianela Rojas, Anna Cattrell, Sergio Vargas-Prada, Matteo Bonzini, Eleni Solidaki, Eda Merisalu, Rima R Habib, Farideh Sadeghian, M Masood Kadir, Sudath Sp Warnakulasuriya, Ko Matsudaira, Busisiwe Nyantumbu-Mkhize, Helen L Kelsall, Helen Harcombe
BACKGROUND: Wide international variation in the prevalence of disabling low back pain (LBP) among working populations is not explained by known risk factors. It would be useful to know whether the drivers of this variation are specific to the spine or factors that predispose to musculoskeletal pain more generally. METHODS: Baseline information about musculoskeletal pain and risk factors was elicited from 11,710 participants aged 20-59 years, who were sampled from 45 occupational groups in 18 countries...
June 8, 2018: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/29879901/correction-to-identification-of-two-different-chemosensory-pathways-in-representatives-of-the-genus-halomonas
#13
Ana Florencia Gasperotti, María Victoria Revuelta, Claudia Alicia Studdert, María Karina Herrera Seitz
Following the publication of this article [1], the authors noticed that Fig. 3 was missing. In that figure, one of the numbers corresponding to the Halomonas chemoreceptors was missing: namely, chemoreceptor 07070. The correct version of Fig. 3 has been included in this Correction.
June 7, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29879173/exploring-karyotype-diversity-of-argentinian-guaran%C3%A3-maize-landraces-relationship-among-south-american-maize
#14
María Florencia Realini, Lidia Poggio, Julián Cámara Hernández, Graciela Esther González
In Argentina there are two different centers of maize diversity, the Northeastern (NEA) and the Northwestern (NWA) regions of the country. In NEA, morphological studies identified 15 landraces cultivated by the Guaraní communities in Misiones Province. In the present study we analyzed the karyotype diversity of 20 populations of Guaraní maize landraces through classical and molecular cytogenetic analyses. Our results demonstrate significant intra and inter-populational variation in the percentage, number, size, chromosome position and frequencies of the heterochromatic blocks, which are called knobs...
2018: PloS One
https://www.readbyqxmd.com/read/29876606/ecxyl43-%C3%AE-xylosidase-molecular-modeling-activity-on-natural-and-artificial-substrates-and-synergism-with-endoxylanases-for-lignocellulose-deconstruction
#15
Ornella M Ontañon, Silvina Ghio, Rubén Marrero Díaz de Villegas, Florencia E Piccinni, Paola M Talia, María L Cerutti, Eleonora Campos
Biomass hydrolysis constitutes a bottleneck for the biotransformation of lignocellulosic residues into bioethanol and high-value products. The efficient deconstruction of polysaccharides to fermentable sugars requires multiple enzymes acting concertedly. GH43 β-xylosidases are among the most interesting enzymes involved in hemicellulose deconstruction into xylose. In this work, the structural and functional properties of β-xylosidase EcXyl43 from Enterobacter sp. were thoroughly characterized. Molecular modeling suggested a 3D structure formed by a conserved N-terminal catalytic domain linked to an ancillary C-terminal domain...
June 6, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29875136/trastuzumab-inhibits-pituitary-tumor-growth-modulating-the-tgfb-smad2-3-pathway
#16
Juan Pablo Petiti, Liliana Del Valle Sosa, Florencia Picech, Gabriela Deisi Moyano Crespo, Jean Zander Arevalo Rojas, Pablo Anibal Pérez, Carolina Beatriz Guido, Carolina Leimgruber, María Eugenia Sabatino, Pedro Emilio García, Verónica Bengió, Francisco Roque Papalini, Paula Estario, Maria Celina Bernhardt, Marcos Villarreal, Silvina Gutiérrez, Ana Lucía De Paul, Jorge Humberto Mukdsi, Alicia I Torres
In pituitary adenomas, early recurrences and resistance to conventional pharmacotherapies are common, but the mechanisms involved are still not understood. The high expression of epidermal growth factor receptor 2 (HER2)/extracellular signal-regulated kinase (ERK1/2) signal observed in human pituitary adenomas, together with the low levels of the antimitogenic transforming growth factor beta receptor 2 (TBR2), encouraged us to evaluate the effect of the specific HER2 inhibition with trastuzumab on experimental pituitary tumor cell growth and its effect on the antiproliferative response to TGFB1...
June 6, 2018: Endocrine-related Cancer
https://www.readbyqxmd.com/read/29870713/actin-network-discussion-during-mitotic-pseudo-furrowing
#17
Florencia di Pietro, Yohanns Bellaïche
Two types of cortical actin networks act during mitotic pseudocleavage furrowing in the Drosophila syncytium, but how they interact has remained elusive. In this issue of Developmental Cell, Zhang et al. (2018) show how these networks shape each other and propose that furrowing is driven by actin polymerization-derived pushing forces.
June 4, 2018: Developmental Cell
https://www.readbyqxmd.com/read/29867077/the-mucosal-surfaces-of-both-eyes-are-immunologically-linked-by-a-neurogenic-inflammatory-reflex-involving-trpv1-and-substance-p
#18
Mauricio Guzmán, Maximiliano S Miglio, Nadia R Zgajnar, Ana Colado, María B Almejún, Irene A Keitelman, Florencia Sabbione, Federico Fuentes, Analía S Trevani, Mirta N Giordano, Jeremías G Galletti
Immunological interdependence between the two eyes has been reported for the cornea and the retina but not for the ocular mucosal surface. Intriguingly, patients frequently report ocular surface-related symptoms in the other eye after unilateral ocular surgery. Here we show how unilateral eye injuries in mice affect the mucosal immune response of the opposite ocular surface. We report that, despite the lack of lymphatic cross-drainage, a neurogenic inflammatory reflex in the contralateral conjunctiva is sufficient to increase, first, epithelial nuclear factor kappa B signaling, then, dendritic cell maturation, and finally, expansion of effector, instead of regulatory, T cells in the draining lymph node, leading to disrupted ocular mucosal tolerance...
June 4, 2018: Mucosal Immunology
https://www.readbyqxmd.com/read/29806679/-diagnostic-accuracy-of-serum-protein-electrophoresis-and-free-light-chain-measurements-for-monoclonal-gammopathies
#19
Camila Peña, Manuela Ortiz, Javier Voisin, Alexis Peralta, Viviana Balboa, Florencia Delgado
BACKGROUND: International guidelines suggest a screening panel for monoclonal gammopathies that contains serum protein electrophoresis (SPE), free light chain (FLC) measurements and immunofixation. This combination provides the possibility of a timely accurate diagnosis. AIM: To evaluate the sensibility of a simple screening panel (SPE + FLC). MATERIAL AND METHODS: We analyzed 191 consecutive serum samples of patients with a suspected monoclonal gammopathy (MG)...
January 2018: Revista Médica de Chile
https://www.readbyqxmd.com/read/29801819/cellulose-nanofibers-from-banana-peels-as-a-pickering-emulsifier-high-energy-emulsification-processes
#20
Ana Letícia Rodrigues Costa, Andresa Gomes, Heloisa Tibolla, Florencia Cecilia Menegalli, Rosiane Lopes Cunha
Cellulose nanofibers (CNFs) from banana peels was evaluated as promising stabilizer for oil-in-water emulsions. CNFs were treated using ultrasound and high-pressure homogenizer. Changes on the size, crystallinity index and zeta potential of CNFs were associated with the intense effects of cavitation phenomenon and shear forces promoted by mechanical treatments. CNFs-stabilized emulsions were produced under the same process conditions as the particles. Coalescence phenomenon was observed in the emulsions produced using high-pressure homogenizer, whereas droplets flocculation occurred in emulsions processed by ultrasound...
August 15, 2018: Carbohydrate Polymers
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