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https://www.readbyqxmd.com/read/29143769/senescent-nephropathy-the-new-renal-syndrome
#1
REVIEW
Florencia Aiello, Eliana P Dueñas, Carlos G Musso
Chronic kidney disease (CKD) is a condition characterized by progressive and irreversible deterioration of renal function due to the reduction of nephron mass for a period of at least three months. The prevalence of CKD is roughly 10% in the general population but increases with age, affecting more than one-third of people older than 65. Frailty is a condition usually found in elderly people, characterized by weakness, motility, and balance issues, with a declined ability to resist stressors leading to increased risks of adverse health outcomes including falls, fracture, hospitalization, institutionalization, disability, dependence, dementia, poor quality of life, and death...
October 28, 2017: Healthcare (Basel, Switzerland)
https://www.readbyqxmd.com/read/29143226/aligned-ovine-diaphragmatic-myoblasts-overexpressing-human-connexin-43-seeded-on-poly-l-lactic-acid-scaffolds-for-potential-use-in-cardiac-regeneration
#2
Carlos Sebastián Giménez, Paola Locatelli, Florencia Montini Ballarin, Alejandro Orlowski, Ricardo A Dewey, Milagros Pena, Gustavo Abel Abraham, Ernesto Alejandro Aiello, María Del Rosario Bauzá, Luis Cuniberti, Fernanda Daniela Olea, Alberto Crottogini
Diaphragmatic myoblasts (DMs) are precursors of type-1 muscle cells displaying high exhaustion threshold on account that they contract and relax 20 times/min over a lifespan, making them potentially useful in cardiac regeneration strategies. Besides, it has been shown that biomaterials for stem cell delivery improve cell retention and viability in the target organ. In the present study, we aimed at developing a novel approach based on the use of poly (L-lactic acid) (PLLA) scaffolds seeded with DMs overexpressing connexin-43 (cx43), a gap junction protein that promotes inter-cell connectivity...
November 15, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29136841/miniaturized-quechers-based-methodology-for-multiresidue-determination-of-pesticides-in-odonate-nymphs-as-ecosystem-biomonitors
#3
Florencia Jesús, Ricardo Hladki, Natalia Gérez, Natalia Besil, Silvina Niell, Grisel Fernández, Horacio Heinzen, María Verónica Cesio
The impacts of the modern, agrochemicals based agriculture that threatens the overall systems sustainability, need to be monitored and evaluated. Seeking for agroecosystems monitors, the present article focus in the occurrence and abundance of aquatic macroinvertebrates, that have been frequently used as bioindicators of water quality due to their relationship with land use. Some of these organisms are on the top of the food chain, where bioaccumulation and biomagnification processes can be observed, and they can turn into secondary pollution sources of systems and terrestrial organisms as well...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29131603/towards-an-understanding-of-selective-alkyne-hydrogenation-on-ceria-on-the-impact-of-o-vacancies-on-h2-interaction-with-ceo2-111
#4
Kristin Werner, Xuefei Weng, Florencia Calaza, Martin Sterrer, Thomas Kropp, Joachim Paier, Joachim Sauer, Markus Wilde, Katsuyuki Fukutani, Shamil Shaikhutdinov, Hans-Joachim Freund
Ceria (CeO2) has recently been found to be a promising catalyst in the selective hydrogenation of alkynes to alkenes. This reaction occurs primarily on highly dispersed metal catalysts, but rarely on oxide surfaces. The origin of the outstanding activity and selectivity observed on CeO2 remains unclear. In this work, we show that one key aspect of the hydrogenation reaction - the interaction of hydrogen with the oxide - depends strongly on the presence of O vacancies within CeO2. Through infrared reflection absorption spectroscopy on well-ordered CeO2(111) thin films and density functional theory (DFT) calculations, we show that the preferred heterolytic dissociation of molecular hydrogen on CeO2(111) requires H2 pressures in the mbar regime...
November 13, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29130964/changes-in-the-proliferation-apoptosis-balance-in-the-bovine-ovary-a-key-early-event-in-follicular-persistence
#5
E Matías Belotti, Antonela F Stassi, Melisa M L Velázquez, Pablo U Díaz, Belkis E Marelli, Florencia Rey, Ulises S Notaro, Hugo H Ortega, Natalia R Salvetti
The objective of this work was to evaluate proliferation and apoptosis in the bovine ovary in a model of follicular persistence induced by low levels of progesterone to detect incipient changes during cystic ovarian disease development on the expected day of ovulation (day 0) and after 5, 10, and 15 days of follicular persistence. We analyzed cell proliferation by evaluating the expression of Ki-67 and apoptosis by evaluating caspase-3, BAX, and BCL2 expression. Proliferation was similar in the granulosa and theca cells of antral follicles in the P0 group (treated with progesterone up to the expected day of ovulation) and in the control group...
November 3, 2017: Cells, Tissues, Organs
https://www.readbyqxmd.com/read/29127872/biomonitoring-levels-and-trends-of-pahs-and-synthetic-musks-associated-with-land-use-in-urban-environments
#6
Ivan Tavera Busso, Florencia Tames, José Avelino Silva, Sara Ramos, Vera Homem, Nuno Ratola, Hebe Carreras
Polycyclic aromatic hydrocarbons (PAHs) are some of the most studied organic compounds in urban environments, due to their known adverse effects on human health and persistence in environmental matrices. During the last decade, new groups of organic compounds with an intensive use worldwide such as synthetic musks have been raising the interest of the scientific community given their toxicity and health effects. However, literature is still scarce in studies dealing with their concentration in the environment, especially in developing countries, where they are even more rare or non-existing at all...
November 8, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29126886/platinum-palladium-rhodium-molybdenum-and-strontium-in-blood-of-urban-women-in-nine-countries
#7
Gerda Rentschler, Ilia Rodushkin, Milena Cerna, Chunying Chen, Florencia Harari, Raúl Harari, Milena Horvat, Frantiska Hruba, Lucie Kasparova, Kvetoslava Koppova, Andrea Krskova, Mladen Krsnik, Jawhar Laamech, Yu-Feng Li, Lina Löfmark, Thomas Lundh, Nils-Göran Lundström, Badiaa Lyoussi, Darja Mazej, Josko Osredkar, Krystyna Pawlas, Natalia Pawlas, Adam Prokopowicz, Staffan Skerfving, Janja Snoj Tratnik, Vera Spevackova, Zdravko Spiric, Anneli Sundkvist, Ulf Strömberg, Drazenka Vadla, Katerina Wranova, Soumia Zizi, Ingvar A Bergdahl
BACKGROUND: There is little reliable information on human exposure to the metals platinum (Pt), palladium (Pd) and rhodium (Rh), despite their use in enormous quantities in catalytic converters for automobile exhaust systems. OBJECTIVES: To evaluate blood concentrations of Pt (B-Pt), Pd (B-Pd) and Rh (B-Rh) in women from six European and three non-European countries, and to identify potentially influential factors. In addition, molybdenum (Mo) and strontium (Sr) were analysed...
November 2, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29121844/patient-and-aneurysm-characteristics-predicting-prolonged-length-of-stay-after-elective-open-aaa-repair-in-the-endovascular-era
#8
Sergio Casillas-Berumen, Florencia A Rojas-Miguez, Alik Farber, Sevan Komshian, Jeffrey A Kalish, Denis Rybin, Gheorghe Doros, Jeffrey J Siracuse
INTRODUCTION: Open aortic aneurysm repair (AAA) repair can be resource intensive and associated with a prolonged length of stay (LOS). We sought to examine patient and aneurysm predictors of prolonged LOS to better identify those at risk in the preoperative setting. METHODS: Patient data were obtained from the targeted AAA American College of Surgery National Surgical Quality Improvement Program database from 2012 to 2014 of patients undergoing open AAA repair. Multivariable logistic regression was used to determine predictors of prolonged postoperative LOS defined as greater than 10 days (75th percentile)...
January 1, 2017: Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/29116706/effect-of-sperm-dna-fragmentation-on-embryo-development-clinical-and-biological-aspects
#9
Cristian Alvarez Sedó, Melina Bilinski, Daniela Lorenzi, Heydy Uriondo, Felicitas Noblía, Valeria Longobucco, Estefanía Ventimiglia Lagar, Florencia Nodar
OBJECTIVE: The aim of this study was to investigate the effect of sperm DNA fragmentation on fertilization rate, embryo development (blastulation rate), and pregnancy outcomes for ICSI cycles performed in a cohort of couples using donor eggs and to assess the remaining embryos that were not transferred or frozen for apoptotic markers. METHODS: Eighty-two women (egg recipients) were included in the study (2016) were included in the study. The recipients' mean age was 41...
November 8, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29107437/selenium-containing-analogues-of-wc-9-are-extremely-potent-inhibitors-of-trypanosoma-cruzi-proliferation
#10
María N Chao, Melissa Storey, Catherine Li, Maricel G Rodríguez, Florencia Di Salvo, Sergio H Szajnman, Silvia N J Moreno, Roberto Docampo, Juan B Rodriguez
The obligate intracellular parasite, Trypanosoma cruzi is the etiologic agent of Chagas disease or American trypanosomiasis, which is the most prevalent parasitic disease in the Americas. The present chemotherapy to control this illness is still deficient particularly in the chronic stage of the disease. The ergosterol biosynthesis pathway has received much attention as a molecular target for the development of new drugs for Chagas disease. Especially, inhibitors of the enzymatic activity of squalene synthase were shown to be effective compounds on T...
October 16, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29097729/akap350-recruits-eb1-to-the-spindle-poles-ensuring-proper-spindle-orientation-and-lumen-formation-in-3d-epithelial-cell-cultures
#11
Evangelina Almada, Facundo M Tonucci, Florencia Hidalgo, Anabela Ferretti, Solange Ibarra, Alejandro Pariani, Rodrigo Vena, Cristián Favre, Javier Girardini, Arlinet Kierbel, M Cecilia Larocca
The organization of epithelial cells to form hollow organs with a single lumen requires the accurate three-dimensional arrangement of cell divisions. Mitotic spindle orientation is defined by signaling pathways that provide molecular links between specific spots at the cell cortex and astral microtubules, which have not been fully elucidated. AKAP350 is a centrosomal/Golgi scaffold protein, implicated in the regulation of microtubule dynamics. Using 3D epithelial cell cultures, we found that cells with decreased AKAP350 expression (AKAP350KD) formed polarized cysts with abnormal lumen morphology...
November 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29095137/burkholderia-puraquae-sp-nov-a-novel-species-of-the-burkholderia-cepacia-complex-isolated-from-hospital-settings-and-agricultural-soils
#12
Pablo Martina, Mariana Leguizamon, Claudia I Prieto, Silvia A Sousa, Patricia Montanaro, Walter O Draghi, Maren Stämmler, Marisa Bettiol, Carla C C R de Carvalho, Juliana Palau, Cecilia Figoli, Florencia Alvarez, Silvina Benetti, Sergio Lejona, Cecilia Vescina, Julián Ferreras, Peter Lasch, Antonio Lagares, Angeles Zorreguieta, Jorge H Leitão, Osvaldo M Yantorno, Alejandra Bosch
Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by more than 3 % from those of the Bcc with validly published names...
November 2, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29080789/apicomplexan-profilins-in-vaccine-development-applied-to-bovine-neosporosis
#13
Florencia C Mansilla, Alejandra V Capozzo
Neospora caninum, an intracellular protozoan parasite from the phylum Apicomplexa, is the etiologic agent of neosporosis, a disease considered as a major cause of reproductive loss in cattle and neuromuscular disease in dogs. Bovine neosporosis has a great economic impact in both meat and dairy industries, related to abortion, premature culling and reduced milk yields. Although many efforts have been made to restrain bovine neosporosis, there are still no efficacious control methods. Many vaccine-development studies focused in the apicomplexan proteins involved in the adhesion and invasion of the host cell...
October 31, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29075647/systematic-standardized-and-individualized-assessment-of-masticatory-cycles-using-electromagnetic-3d-articulography-and-computer-scripts
#14
Ramón Fuentes, Alain Arias, María Florencia Lezcano, Diego Saravia, Gisaku Kuramochi, Fernando José Dias
Masticatory movements are studied for decades in odontology; a better understanding of them could improve dental treatments. The aim of this study was to describe an innovative, accurate, and systematic method of analyzing masticatory cycles, generating comparable quantitative data. The masticatory cycles of 5 volunteers (Class I, 19 ± 1.7 years) without articular or dental occlusion problems were evaluated using 3D electromagnetic articulography supported by MATLAB software. The method allows the trajectory morphology of the set of chewing cycles to be analyzed from different views and angles...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29073215/toxoplasma-gondii-serine-protease-inhibitor-1-a-new-adjuvant-candidate-for-asthma-therapy
#15
Ariadna S Soto, Ignacio M Fenoy, Vanesa R Sanchez, Florencia March, Matías D Perrone Sibilia, María de Los Angeles Aldirico, Mariano S Picchio, Nadia Arcon, Patricio L Acosta, Fernando P Polack, Valentina Martin, Alejandra Goldman
Serine-proteases are important players in the pathogenesis of asthma, promoting inflammation and tissue remodeling. It's also known that many serine protease inhibitors display immunomodulatory properties. TgPI-1 is a Toxoplasma gondii protein that exhibits broad spectrum inhibitory activity against serine proteases. In view of the increased prevalence of atopic disorders and the need to develop new treatment strategies we sought to investigate the potential of TgPI-1 for treating respiratory allergies. For this purpose, we developed a therapeutic experimental model...
2017: PloS One
https://www.readbyqxmd.com/read/29066034/persistence-of-the-same-genetic-type-of-mycoplasma-hyopneumoniae-in-a-closed-herd-for-at-least-two-years
#16
Florencia Rebaque, Pablo Camacho, Julián Parada, Paula Lucchesi, Arnaldo Ambrogi, Pablo Tamiozzo
Two cross-sectional studies were carried out in 2013 and 2015 monitoring for Mycoplasma hyopneumoniae presence in a swine farm. In these studies, the genetic diversity of M. hyopneumoniae was assessed in clinical specimens using a Multiple Locus Variable-number tandem repeat Analysis (MLVA) targeting P97 R1, P146 R3 and H4 loci. The samples from August 2015 showed the MLVA profile prevalent in June 2013, therefore it can be concluded that a same genetic type of M. hyopneumoniae can persist for at least two years in a closed herd...
October 20, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29058171/cavitary-pulmonary-sporotrichosis-case-report-and-literature-review
#17
Florencia D Rojas, Mariana S Fernández, Juan Manuel Lucchelli, Dora Lombardi, José Malet, María Eugenia Vetrisano, María Emilia Cattana, María de Los Ángeles Sosa, Gustavo Giusiano
A case of cavitary pulmonary sporotrichosis without mucocutaneous involvement caused by Sporothrix schenckii is reported in a sexagenarian woman with a long smoking history. The patient was hospitalized for septic shock with multiorgan failure from a respiratory focus. The diagnosis was delayed due to the fungal etiological agent was not initially considered in the differential diagnosis. A good clinical and radiological evolution was obtained with the antifungal therapy. Occasional cases of primary pulmonary sporotrichosis have been reported in the literature...
December 2017: Mycopathologia
https://www.readbyqxmd.com/read/29056670/protein-composition-of-the-bovine-herpesvirus-1-1-virion
#18
Kaley A Barber, Hillary C Daugherty, Stephanie E Ander, Victoria A Jefferson, Leslie A Shack, Tibor Pechan, Bindu Nanduri, Florencia Meyer
Bovine herpesvirus (BoHV) type 1 is an important agricultural pathogen that infects cattle and other ruminants worldwide. Acute infection of the oro-respiratory tract leads to immune suppression and allows commensal bacteria to infect an otherwise healthy lower respiratory tract. This condition is known as the Bovine Respiratory Disease (BRD). BoHV-1 latently infects the host for life and periodical stress events re-initiate BRD, translating into high morbidity and large economic losses. To gain a better understanding of the biology of BoHV-1 and the disease it causes, we elucidated the protein composition of extracellular virions using liquid chromatography-mass spectrometry analysis...
February 20, 2017: Veterinary Sciences
https://www.readbyqxmd.com/read/29055510/prevalence-of-burkholderia-cepacia-complex-species-in-cystic-fibrosis-patients-in-argentina-during-the-period-2011-2015
#19
Lucía Cipolla, Florencia Rocca, Claudia Martinez, Lorena Aguerre, Rubén Barrios, Mónica Prieto
INTRODUCTION: Burkholderia cepacia (B. cepacia) complex is composed of 20 phylogenetically closely related bacterial species. Some species have emerged as opportunistic pathogens in immunocompromised patients and are responsible for nosocomial outbreaks. The B. cepacia complex is a recognized respiratory pathogen in patients with cystic fibrosis. Burkholderia cenocepacia and Burkholderia multivorans (B. multivorans) are the most prevalent species in the world, according to the literature...
October 18, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29053635/differential-interaction-of-antimicrobial-peptides-with-lipid-structures-studied-by-coarse-grained-molecular-dynamics-simulations
#20
Galo E Balatti, Ernesto E Ambroggio, Gerardo D Fidelio, M Florencia Martini, Mónica Pickholz
In this work; we investigated the differential interaction of amphiphilic antimicrobial peptides with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid structures by means of extensive molecular dynamics simulations. By using a coarse-grained (CG) model within the MARTINI force field; we simulated the peptide-lipid system from three different initial configurations: (a) peptides in water in the presence of a pre-equilibrated lipid bilayer; (b) peptides inside the hydrophobic core of the membrane; and (c) random configurations that allow self-assembled molecular structures...
October 20, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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