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https://www.readbyqxmd.com/read/28323681/predictors-that-a-diagnosis-of-mild-cognitive-impairment-will-remain-stable-3-years-later
#1
Matthew A Clem, Ryan P Holliday, Seema Pandya, Linda S Hynan, Laura H Lacritz, Fu L Woon
BACKGROUND AND OBJECTIVE: In half to two thirds of patients who are diagnosed with mild cognitive impairment (MCI), the diagnosis neither converts to dementia nor reverts to normal cognition; however, little is known about predictors of MCI stability. Our study aimed to identify those predictors. METHODS: We obtained 3-year longitudinal data from the National Alzheimer's Coordinating Center Uniform Data Set for patients with a baseline diagnosis of MCI. To predict MCI stability, we used the patients' baseline data to conduct three logistic regression models: demographics, global function, and neuropsychological performance...
March 2017: Cognitive and Behavioral Neurology: Official Journal of the Society for Behavioral and Cognitive Neurology
https://www.readbyqxmd.com/read/28317888/direct-evidence-of-lack-of-colocalisation-of-fluorescently-labelled-gold-labels-used-in-correlative-light-electron-microscopy
#2
Benjamin T Miles, Alexander B Greenwood, David Benito-Alifonso, Hugh Tanner, M Carmen Galan, Paul Verkade, Henkjan Gersen
Fluorescently labelled nanoparticles are routinely used in Correlative Light Electron Microscopy (CLEM) to combine the capabilities of two separate microscope platforms: fluorescent light microscopy (LM) and electron microscopy (EM). The inherent assumption is that the fluorescent label observed under LM colocalises well with the electron dense nanoparticle observed in EM. Herein we show, by combining single molecule fluorescent imaging with optical detection of the scattering from single gold nanoparticles, that for a commercially produced sample of 10 nm gold nanoparticles tagged to Alexa-633 there is in fact no colocalisation between the fluorescent signatures of Alexa-633 and the scattering associated with the gold nanoparticle...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28278087/laccase-immobilized-dendritic-nanofibrous-membranes-as-a-novel-approach-towards-the-removal-of-bisphenol-a
#3
Lebohang E Koloti, Nonjabulo P Gule, Omotayo A Arotiba, Soraya P Malinga
Laccase enzymes from Rhus vernificera were covalently bound on hyperbranched polyethyleneimine/polyethersulfone (HPEI/PES) electrospun nanofibrous membranes and used for the removal of bisphenol A (BPA) from water. The laccase enzyme was anchored on the dendritic membranes through the abundant peripheral amine groups on the hyperbranched polyethyleneimine (HPEI) using glutaraldehyde as a cross-linker. The membranes were characterised with attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDS) and ultraviolet-visible (UV-Vis) spectroscopy and correlative light and electron microscopy (CLEM)...
March 2, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28230492/clem-analysis-of-the-angiogenic-process-in-a-zebrafish-model-of-spinal-cord-injury
#4
A Pinto, A Ribeiro, T Carvalho, M L Saúde
No abstract text is available yet for this article.
January 2017: Ultrastructural Pathology
https://www.readbyqxmd.com/read/28193691/optogenetic-examination-of-prefrontal-amygdala-synaptic-development
#5
Maithe Arruda-Carvalho, Wan-Chen Wu, Kirstie A Cummings, Roger L Clem
A brain network comprising the medial prefrontal cortex (mPFC) and amygdala plays important roles in developmentally regulated cognitive and emotional processes. However, very little is known about the maturation of mPFC-amygdala circuitry. We conducted anatomical tracing of mPFC projections and optogenetic interrogation of their synaptic connections with neurons in the basolateral amygdala (BLA) at neonatal to adult developmental stages in mice. Results indicate that mPFC-BLA projections exhibit delayed emergence relative to other mPFC pathways and establish synaptic transmission with BLA excitatory and inhibitory neurons in late infancy, events that coincide with a massive increase in overall synaptic drive...
March 15, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28154417/a-global-approach-for-quantitative-super-resolution-and-electron-microscopy-on-cryo-and-epoxy-sections-using-self-labeling-protein-tags
#6
Andreas Müller, Martin Neukam, Anna Ivanova, Anke Sönmez, Carla Münster, Susanne Kretschmar, Yannis Kalaidzidis, Thomas Kurth, Jean-Marc Verbavatz, Michele Solimena
Correlative light and electron microscopy (CLEM) is a powerful approach to investigate the molecular ultrastructure of labeled cell compartments. However, quantitative CLEM studies are rare, mainly due to small sample sizes and the sensitivity of fluorescent proteins to strong fixatives and contrasting reagents for EM. Here, we show that fusion of a self-labeling protein to insulin allows for the quantification of age-distinct insulin granule pools in pancreatic beta cells by a combination of super resolution and transmission electron microscopy on Tokuyasu cryosections...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28139674/ec-clem-flexible-multidimensional-registration-software-for-correlative-microscopies
#7
Perrine Paul-Gilloteaux, Xavier Heiligenstein, Martin Belle, Marie-Charlotte Domart, Banafshe Larijani, Lucy Collinson, Graça Raposo, Jean Salamero
No abstract text is available yet for this article.
January 31, 2017: Nature Methods
https://www.readbyqxmd.com/read/28054419/identification-and-initial-characterization-of-matrix-metalloproteinases-in-the-yellow-fever-mosquito-aedes-aegypti
#8
A M Kantor, S Dong, N L Held, E Ishimwe, A L Passarelli, R J Clem, A W E Franz
Aedes aegypti is a major vector for arboviruses such as dengue, chikungunya and Zika viruses. During acquisition of a viremic bloodmeal, an arbovirus infects mosquito midgut cells before disseminating to secondary tissues, including the salivary glands. Once virus is released into the salivary ducts it can be transmitted to another vertebrate host. The midgut is surrounded by a basal lamina (BL) in the extracellular matrix, consisting of a proteinaceous mesh composed of collagen IV and laminin. BL pore size exclusion limit prevents virions from passing through...
February 2017: Insect Molecular Biology
https://www.readbyqxmd.com/read/28011168/toxoplasma-gondii-plaque-assays-revisited-improvements-for-ultrastructural-and-quantitative-evaluation-of-lytic-parasite-growth
#9
Christoph-Martin Ufermann, Florian Müller, Nora Frohnecke, Michael Laue, Frank Seeber
Lytic growth of intracellular Toxoplasma gondii tachyzoite stages over a period of days results in plaques within mononolayers of host cells. Plaque assays are in frequent use to isolate single clones and to investigate invasion, replication and egress over a longer time frame. To allow correlating plaque morphology and/or size with ultrastructural examination of individual parasites we introduce a simple protocol for correlative light and electron microscopy (CLEM) of entire plaques. We also illustrate the advantages of visualizing only the boundaries of plaques by staining for infected cells ('positive staining') rather than the traditional staining of the intact cell monolayer, thus outlining the area of lysed cells ('negative staining')...
December 21, 2016: Experimental Parasitology
https://www.readbyqxmd.com/read/27977021/correlated-fluorescence-microscopy-and-cryo-electron-tomography-of-virus-infected-or-transfected-mammalian-cells
#10
Cheri M Hampton, Joshua D Strauss, Zunlong Ke, Rebecca S Dillard, Jason E Hammonds, Eric Alonas, Tanay M Desai, Mariana Marin, Rachel E Storms, Fredrick Leon, Gregory B Melikyan, Philip J Santangelo, Paul W Spearman, Elizabeth R Wright
Correlative light and electron microscopy (CLEM) combines spatiotemporal information from fluorescence light microscopy (fLM) with high-resolution structural data from cryo-electron tomography (cryo-ET). These technologies provide opportunities to bridge knowledge gaps between cell and structural biology. Here we describe our protocol for correlated cryo-fLM, cryo-electron microscopy (cryo-EM), and cryo-ET (i.e., cryo-CLEM) of virus-infected or transfected mammalian cells. Mammalian-derived cells are cultured on EM substrates, using optimized conditions that ensure that the cells are spread thinly across the substrate and are not physically disrupted...
January 2017: Nature Protocols
https://www.readbyqxmd.com/read/27974202/the-drosophila-clock-neuron-network-features-diverse-coupling-modes-and-requires-network-wide-coherence-for-robust-circadian-rhythms
#11
Zepeng Yao, Amelia J Bennett, Jenna L Clem, Orie T Shafer
In animals, networks of clock neurons containing molecular clocks orchestrate daily rhythms in physiology and behavior. However, how various types of clock neurons communicate and coordinate with one another to produce coherent circadian rhythms is not well understood. Here, we investigate clock neuron coupling in the brain of Drosophila and demonstrate that the fly's various groups of clock neurons display unique and complex coupling relationships to core pacemaker neurons. Furthermore, we find that coordinated free-running rhythms require molecular clock synchrony not only within the well-characterized lateral clock neuron classes but also between lateral clock neurons and dorsal clock neurons...
December 13, 2016: Cell Reports
https://www.readbyqxmd.com/read/27943680/progression-of-lung-disease-in-preschool-patients-with-cystic-fibrosis
#12
Sanja Stanojevic, Stephanie D Davis, George Retsch-Bogart, Hailey Webster, Miriam Davis, Robin C Johnson, Renee Jensen, Maria Ester Pizarro, Mica Kane, Charles C Clem, Leah Schornick, Padmaja Subbarao, Felix A Ratjen
RATIONALE: Implementation of intervention strategies to prevent lung damage in early cystic fibrosis (CF) requires objective outcome measures that capture and track lung disease. OBJECTIVE: To define the utility of the Lung Clearance Index (LCI), measured by Multiple Breath Washout (MBW), as a means to track disease progression in preschool children with CF. METHODS: CF children between the ages of 2.5 and 6 years with a confirmed diagnosis of CF and age-matched healthy controls were enrolled at three North American CF centers...
December 12, 2016: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/27917008/visualization-of-neutrophil-extracellular-traps-and-fibrin-meshwork-in-human-fibrinopurulent-inflammatory-lesions-iii-correlative-light-and-electron-microscopic-study
#13
Takanori Onouchi, Kazuya Shiogama, Yasuyoshi Mizutani, Takashi Takaki, Yutaka Tsutsumi
Neutrophil extracellular traps (NETs) released from dead neutrophils at the site of inflammation represent webs of neutrophilic DNA stretches dotted with granule-derived antimicrobial proteins, including lactoferrin, and play important roles in innate immunity against microbial infection. We have shown the coexistence of NETs and fibrin meshwork in varied fibrinopurulent inflammatory lesions at both light and electron microscopic levels. In the present study, correlative light and electron microscopy (CLEM) employing confocal laser scanning microscopy and scanning electron microscopy was performed to bridge light and electron microscopic images of NETs and fibrin fibrils in formalin-fixed, paraffin-embedded, autopsied lung sections of legionnaire's pneumonia...
November 1, 2016: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/27862974/phenotypic-variation-in-food-utilization-in-an-outbreak-insect-herbivore
#14
Roberto Quezada-García, Álvaro Fuentealba, Éric Bauce
The effects of nutrition may have subtantial impact on insect evolution by shaping different components of phenotypes. The key to undestanding this evolutionary process is to know how nutritional condition affects additive and non-additive components of the phenotype. However, this is poorly understood in outbreaking insects. We investigated the additive and non-additive variation present in food utilization traits in spruce budworm individuals subjected to chronic nutritional stress. 160 full-sib families of spruce budworm (Choristoneura fumiferana Clem...
November 8, 2016: Insect Science
https://www.readbyqxmd.com/read/27855547/traumatic-brain-injury-history-is-associated-with-earlier-age-of-onset-of-alzheimer-disease
#15
Christian LoBue, Hannah Wadsworth, Kristin Wilmoth, Matthew Clem, John Hart, Kyle B Womack, Nyaz Didehbani, Laura H Lacritz, Heidi C Rossetti, C Munro Cullum
OBJECTIVE: This study examined whether a history of traumatic brain injury (TBI) is associated with earlier onset of Alzheimer disease (AD), independent of apolipoprotein ε4 status (Apoe4) and gender. METHOD: Participants with a clinical diagnosis of AD (n = 7625) were obtained from the National Alzheimer's Coordinating Center Uniform Data Set, and categorized based on self-reported lifetime TBI with loss of consciousness (LOC) (TBI+ vs. TBI-) and presence of Apoe4...
January 2017: Clinical Neuropsychologist
https://www.readbyqxmd.com/read/27851087/1451-norepinephrine-and-vasopressin-vs-norepinephrine-alone-for-septic-shock-retrospective-cohort-study
#16
Drayton Hammond, Julia Cullen, Kelsey McCain, Oktawia Clem, Amy Brotherton, Divyan Chopra, Nikhil Meena, Jacob Painter
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27851068/1432-discontinuation-of-vasopressin-before-norepinephrine-in-recovery-phase-of-septic-shock-cohort-study
#17
Drayton Hammond, Oktawia Clem, Amy Brotherton, Kelsey McCain, Julia Cullen, Divyan Chopra, Nikhil Meena, Jacob Painter
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27850986/1350-norepinephrine-and-vasopressin-vs-norepinephrine-alone-for-septic-shock-randomized-controlled-trial
#18
Oktawia Clem, Jacob Painter, Julia Cullen, Kelsey McCain, Krishna Kakkera, Nikhil Meena, Drayton Hammond
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27847357/visualization-of-hiv-t-cell-virological-synapses-and-virus-containing-compartments-by-three-dimensional-correlative-light-and-electron-microscopy
#19
Lili Wang, Edward T Eng, Kenneth Law, Ronald E Gordon, William J Rice, Benjamin K Chen
Virological synapses (VS) are adhesive structures that form between infected and uninfected cells to enhance the spread of HIV-1. During T cell VS formation, viral proteins are actively recruited to the site of cell-cell contact where the viral material is efficiently translocated to target cells into heterogeneous, protease-resistant, antibody-inaccessible compartments. Using correlative light and electron microscopy (CLEM), we define the membrane topography of the virus-containing compartments (VCC) where HIV is found following VS-mediated transfer...
January 15, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27834401/3-d-em-exploration-of-the-hepatic-microarchitecture-lessons-learned-from-large-volume-in-situ-serial-sectioning
#20
Gerald John Shami, Delfine Cheng, Minh Huynh, Celien Vreuls, Eddie Wisse, Filip Braet
To-date serial block-face scanning electron microscopy (SBF-SEM) dominates as the premier technique for generating three-dimensional (3-D) data of resin-embedded biological samples at an unprecedented depth volume. Given the infancy of the technique, limited literature is currently available regarding the applicability of SBF-SEM for the ultrastructural investigation of tissues. Herein, we provide a comprehensive and rigorous appraisal of five different SBF-SEM sample preparation protocols for the large-volume exploration of the hepatic microarchitecture at an unparalleled X, Y and Z resolution...
November 11, 2016: Scientific Reports
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