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Ching-Hao Li, Chen-Wei Liu, Chi-Hao Tsai, Yi-Jen Peng, Yu-Hsuan Yang, Po-Lin Liao, Chen-Chen Lee, Yu-Wen Cheng, Jaw-Jou Kang
Aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor, has been studied extensively in carcinogenesis through the genomic pathway. In recent years, AHR has also been reported to exert positive or negative effects on epithelial-mesenchymal transition (EMT), the crucial step in tumor malignant progression. However, the detailed mechanism remains controversial. Analysis of AHR-expression levels in non-small cell lung cancer cell lines and lung cancer tissues revealed an inverse correlation between AHR protein levels and tumor cell invasion and metastasis...
October 17, 2016: Archives of Toxicology
Raphaela Mayerhofer, Esther E Fröhlich, Florian Reichmann, Aitak Farzi, Nora Kogelnik, Eleonore Fröhlich, Wolfgang Sattler, Peter Holzer
Microbial metabolites are known to affect immune system, brain, and behavior via activation of pattern recognition receptors such as Toll-like receptor 4 (TLR4). Unlike the effect of the TLR4 agonist lipopolysaccharide (LPS), the role of other TLR agonists in immune-brain communication is insufficiently understood. We therefore hypothesized that the TLR2 agonist lipoteichoic acid (LTA) causes immune activation in the periphery and brain, stimulates the hypothalamic-pituitary-adrenal (HPA) axis and has an adverse effect on blood-brain barrier (BBB) and emotional behavior...
October 14, 2016: Brain, Behavior, and Immunity
Josep M Llibre, Alessandro Cozzi-Lepri, Court Pedersen, Matti Ristola, Marcelo Losso, Amanda Mocroft, Viktar Mitsura, Karolin Falconer, Fernando Maltez, Marek Beniowski, Vincenzo Vullo, Gamal Hassoun, Elena Kuzovatova, János Szlavik, Anastasiia Kuznetsova, Hans-Jürgen Stellbrink, Claudine Duvivier, Simon Edwards, Kamilla Laut, Roger Paredes
Effectiveness data of an unboosted atazanavir (ATV) with abacavir/lamivudine (ABC/3TC) switch strategy in clinical routine are scant.We evaluated treatment outcomes of ATV + ABC/3TC in pretreated subjects in the EuroSIDA cohort when started with undetectable plasma HIV-1 viral load (pVL), performing a time to loss of virological response (TLOVR <50 copies/mL) and a snapshot analysis at 48, 96, and 144 weeks. Virological failure (VF) was defined as confirmed pVL >50 copies/mL.We included 285 subjects, 67% male, with median baseline CD4 530 cells, and 44 months with pVL ≤50 copies/mL...
October 2016: Medicine (Baltimore)
Y-B Yu, D-Y Zhao, Q-Q Qi, X Long, X Li, F-X Chen, X-L Zuo
BACKGROUND: Brain-derived neurotrophic factor (BDNF) may play a vital role in the homeostatic regulation of intestinal barrier integrity. We aimed to investigate the physiological role of BDNF in maintaining the intestinal epithelial barrier using postinflammatory irritable bowel syndrome (PI-IBS) mice and explore the underlying molecular mechanisms using intestinal epithelial cells in vitro. METHODS: Postinflammatory-IBS mice were induced by intrarectal administration of trinitrobenzene sulfonic acid and allowed to recover for 28 days...
October 17, 2016: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
Ludmila Khailova, Christine H Baird, Aubri A Rush, Christopher Barnes, Paul E Wischmeyer
BACKGROUND & AIMS: Recent clinical trials and in vivo models demonstrate probiotic administration can reduce occurrence and improve outcome of pneumonia and sepsis, both major clinical challenges worldwide. Potential probiotic benefits include maintenance of gut epithelial barrier homeostasis and prevention of downstream organ dysfunction due to systemic inflammation. However, mechanism(s) of probiotic-mediated protection against pneumonia remain poorly understood. This study evaluated potential mechanistic targets in the maintenance of gut barrier homeostasis following Lactobacillus rhamnosus GG (LGG) treatment in a mouse model of pneumonia...
October 1, 2016: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Adel Dalilottojari, Bahman Delalat, Frances J Harding, Michaelia P Cockshell, Claudine S Bonder, Nicolas H Voelcker
Porous silicon (pSi) substrates are a promising platform for cell expansion, since pore size and chemistry can be tuned to control cell behavior. In addition, a variety of bioactives can be loaded into the pores and subsequently released to act on cells adherent to the substrate. Here we construct a cell microarray on plasma polymer coated pSi substrates that enables the simultaneous culture of human endothelial cells on printed immobilized protein factors while a second soluble growth factor is released from the same substrate...
October 15, 2016: Biomacromolecules
Houmam Belaidi, Salah Belaidi, Claudine Katan, Camille Latouche, Abdou Boucekkine
The electronic and optical properties of six iridium imidazolylidene complexes (1a, 1b, 2, 2b, 3, 3b) that are strong candidates for use in OLED systems were investigated theoretically. Computations using DFT and TD-DFT methods were performed to explain the observed optical properties of these complexes. Observed absorption bands were assigned and the lowest triplet excited states were computed. Whereas complexes 1a and 1b are nonemissive in solution, the simulated phosphorescence spectra of complexes 2, 2b, 3, and 3b were in good agreement with the observed spectra when the vibrational contributions to the electronic transitions were taken into account...
November 2016: Journal of Molecular Modeling
Ajaz A Bhat, Rizwan Ahmad, SrijayaPrakash B Uppada, Amar B Singh, Punita Dhawan
Epithelial-mesenchymal transition (EMT) is an important mechanism in cancer progression and malignancy including colorectal cancer (CRC). Importantly, inflammatory mediators are critical constituents of the local tumor environment and an intimate link between CRC progression and inflammation is now validated. We and others have reported key role of the deregulated claudin-1 expression in colon carcinogenesis including colitis-associated colon cancer (CAC). However, the causal association between claudin-1 expression and inflammation-induced colon cancer progression remains unclear...
October 11, 2016: Experimental Cell Research
Isabelle Poizot-Martin, Clotilde Allavena, Cyrille Delpierre, Claudine Duvivier, Véronique Obry-Roguet, Carla E Cano, Francine Guillouet de Salvador, David Rey, Pierre Dellamonica, Antoine Cheret, Lise Cuzin, Christine Katlama, André Cabié, Bruno Hoen
The aim of the study was to investigate the impact of first-line combined antiretroviral therapy (cART) regimen on the course of CD8 T-cell counts in human immunodeficiency virus (HIV)-infected patients.A retrospective observational study conducted on the French DAT'AIDS Cohort of HIV-infected patients.We selected 605 patients initiating a first-line cART between 2002 and 2009, and which achieved a sustained undetectable HIV plasma viral load (pVL) for at least 12 months without cART modification. The evolution of CD8 T-cell counts according to cART regimen was assessed...
October 2016: Medicine (Baltimore)
Benoît Remenant, Frédéric Borges, Catherine Cailliez-Grimal, Anne-Marie Revol-Junelles, Laurent Marché, Aurélie Lajus, Claudine Médigue, Marie-France Pilet, Hervé Prévost, Monique Zagorec
In this study, we present the draft genome sequence of Carnobacterium divergens V41. This strain was previously reported as producing divercin V41, a bacteriocin of interest for food biopreservation. Its genome revealed also the presence of a gene cluster putatively involved in polyketide production, which is unique in lactic acid bacteria.
October 13, 2016: Genome Announcements
I C Sampaio, P H Q S Medeiros, F A P Rodrigues, P A Cavalcante, S A Ribeiro, J S Oliveira, M M G Prata, D V S Costa, S G C Fonseca, M M Guedes, A M Soares, G A C Brito, A Havt, S R Moore, A A M Lima
Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions...
October 10, 2016: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Naoko Fujii, Yukinobu Matsuo, Toshiyuki Matsunaga, Satoshi Endo, Hideki Sakai, Masahiko Yamaguchi, Yasuhiro Yamazaki, Junko Sugatani, Akira Ikari
Hypotonic stress decreased claudin-1 and -2 expression levels in renal tubular epithelial HK-2 and Madin-Darby canine kidney cells. Here, we examined the regulatory mechanism involved in this decrease. The hypotonic-induced decrease in claudin expression was inhibited by SB202190, a p38 mitogen-activated protein kinase inhibitor, but not by U0126, a MEK inhibitor, Go6983, a protein kinase C inhibitor, or SP600125, a Jun N-terminal protein kinase inhibitor. Hypotonic stress increased transepithelial electrical resistance, which was inhibited by SB202190...
October 12, 2016: Journal of Biological Chemistry
Linqiang Huang, Wei Cao, Yiyu Deng, Gaofeng Zhu, Yongli Han, Hongke Zeng
BACKGROUND: Cerebral oedema is closely related to the permeability of blood-brain barrier, vascular endothelial growth factor (VEGF) and its receptor vascular endothelial growth factor receptor 2 (VEGFR2) all of which are important blood-brain barrier (BBB) permeability regulatory factors. Zonula occludens 1 (ZO-1) and claudin-5 are also the key components of BBB. Hypertonic saline is widely used to alleviate cerebral oedema. This study aimed to explore the possible mechanisms underlying hypertonic saline that ameliorates cerebral oedema effectively...
October 13, 2016: BMC Neuroscience
Silvia L Carvalho, Tatsuya Nagata, Bruna R Junqueira, Larissa G Zanardo, Ana C S Paiva, Claudine M Carvalho
Infectious cDNA clones are an important tool to study the molecular and cellular process of RNA virus infection. In vitro and in vivo transcription systems are the two main strategies used in the generation of infectious cDNA clones for RNA viruses. This study describes the first generation of a full-length infectious cDNA clone of Cowpea mild mottle virus (CPMMV), a Carlavirus. The full-length genome was synthesized by Overlap Extension PCR of two overlapping fragments and cloned in a pUC-based vector under control of the SP6 RNA polymerase promoter...
October 11, 2016: Virus Genes
Kevin Looi, Niamh M Troy, Luke W Garratt, Thomas Iosifidis, Anthony Bosco, Alysia G Buckley, Kak-Ming Ling, Kelly M Martinovich, Elizabeth Kicic-Starcevich, Nicole C Shaw, Erika N Sutanto, Graeme R Zosky, Paul J Rigby, Alexander N Larcombe, Darryl A Knight, Anthony Kicic, Stephen M Stick
RATIONALE: No studies have assessed the effects of human rhinovirus (HRV) infection on epithelial tight junctions (TJs) and resultant barrier function. AIM OF THE STUDY: To correlate viral infection with TJ disassembly, epithelial barrier integrity, and function. MATERIALS AND METHODS: Human airway epithelial cells were infected with HRV minor serotype 1B (HRV-1B) at various 50% tissue culture infectivity doses (TCID50) over 72 hours. HRV replication was assessed by quantitative-polymerase chain reaction (qPCR) while cell viability and apoptosis were assessed by proliferation and apoptotic assays, respectively...
October 11, 2016: Experimental Lung Research
Yueliang Zheng, Wenwei Cai, Shengang Zhou, Liming Xu, Chengxing Jiang
Our study aims to investigate the effects of bone marrow derived mesenchymal stem cells (BM-MSCs) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) as well as the underlying mechanism. In our study, Wistar rats were randomly divided into four groups: control group; ALI group; ALI+MSCs group and ALI+MSCs claudin-4 siRNA group. MRC-5 and BEAS-2B cell lines were used for in vitro assay. Flow cytometry, western blot, hematoxylin and eosin (H&E) staining, CCK-8 assay, enzyme-linked immunosorbent assay (ELISA) were involved to measure the pathological changes in lung tissues...
2016: American Journal of Translational Research
Douglas A Hicks, Carly E Galimanis, Patricia G Webb, Monique A Spillman, Kian Behbakht, Margaret C Neville, Heidi K Baumgartner
BACKGROUND: Claudin-4 is a transmembrane protein expressed at high levels in the majority of epithelial ovarian tumors, irrespective of subtype, and has been associated with tumor cells that are both chemoresistant and highly mobile. The objective of this study was to determine the functional role that claudin-4 plays in apoptosis resistance and migration as well as the therapeutic utility of targeting claudin-4 activity with a small mimic peptide. METHODS: We examined claudin-4 activity in human ovarian tumor cell lines (SKOV3, OVCAR3, PEO4) using in vitro caspase and scratch assays as well as an in vivo mouse model of ovarian cancer...
October 11, 2016: BMC Cancer
Frances Corrigan, Kimberley A Mander, Anna V Leonard, Robert Vink
BACKGROUND: The neuroinflammatory response following traumatic brain injury (TBI) is known to be a key secondary injury factor that can drive ongoing neuronal injury. Despite this, treatments that have targeted aspects of the inflammatory pathway have not shown significant efficacy in clinical trials. MAIN BODY: We suggest that this may be because classical inflammation only represents part of the story, with activation of neurogenic inflammation potentially one of the key initiating inflammatory events following TBI...
October 11, 2016: Journal of Neuroinflammation
Claire Roiland, Gaelle Trippé-Allard, Khaoula Jemli, Bruno Alonso, Jean-Claude Ameline, Régis Gautier, Thierry Bataille, Laurent Le Pollès, Emmanuelle Deleporte, Jacky Even, Claudine Katan
We report on (207)Pb, (79)Br, (14)N, (1)H, (13)C and (2)H NMR experiments for studying the local order and dynamics in hybrid perovskite lattices. (207)Pb NMR experiments conducted at room temperature on a series of MAPbX3 compounds (MA = CH3NH3(+); X = Cl, Br and I) showed that the isotropic (207)Pb NMR shift is strongly dependent on the nature of the halogen ions. Therefore (207)Pb NMR appears to be a very promising tool for the characterisation of local order in mixed halogen hybrid perovskites. (207)Pb NMR on MAPbBr2I served as a proof of concept...
October 5, 2016: Physical Chemistry Chemical Physics: PCCP
Bojan Mirkovic, Claudine Laurent, Marc-Antoine Podlipski, Thierry Frebourg, David Cohen, Priscille Gerardin
Suicidal behaviors (SBs), which range from suicidal ideation to suicide attempts and completed suicide, represent a fatal dimension of mental ill-health. The involvement of genetic risk factors in SB is supported by family, twin, and adoption studies. The aim of this paper is to review recent genetic association studies in SBs including (i) case-control studies, (ii) family-based association studies, and (iii) genome-wide association studies (GWAS). Various studies on genetic associations have tended to suggest that a number of genes [e...
2016: Frontiers in Psychiatry
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