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https://www.readbyqxmd.com/read/28301247/different-duck-species-infected-intramuscularly-with-duck-origin-genotype-ix-apmv-1-show-discrepant-mortality-and-indicate-another-fatal-genotype-apmv-1-to-ducks
#1
Guanghua Fu, Longfei Cheng, Qiuling Fu, Baomin Qi, Cuiteng Chen, Shaohua Shi, Hongmei Chen, Chunhe Wan, Rongchang Liu, Yu Huang
Isolations of genotype IX (gIX) avian paramyxovirus type 1 (APMV-1) from various bird species have been more common recently, with isolates showing variable pathogenicity in different species of poultry. Here we sequenced the genome of a Muscovy duck origin gIX virus strain XBT14 and characterized the virulence and pathogenicity of this isolate in chickens and ducks. The genome sequence of strain XBT14 is 15,192 nt in length, containing multiple basic amino acids at the fusion protein cleavage site. The XBT14 strain shared 91...
March 2017: Avian Diseases
https://www.readbyqxmd.com/read/28288679/muscovy-duck-reovirus-%C3%AF-ns-protein-triggers-autophagy-enhancing-virus-replication
#2
Yijian Wu, Longping Cui, Erpeng Zhu, Wuduo Zhou, Quanxi Wang, Xiaoping Wu, Baocheng Wu, Yifan Huang, Hung-Jen Liu
BACKGROUND: Muscovy duck reovirus (MDRV) causes high morbidity and mortality in Muscovy ducklings at 10 days old and can persist in an infected flock until the ducklings of 6 weeks old. It shares common physicochemical properties with avian reovirus (ARV) and differs in coding assignment and pathogenicity. The ARV p17 protein has been shown to trigger autophagy via activation multiple signaling pathways, which benefits virus replication. Since MDRV lacks the p17 protein, whether and how MDRV induces autophagy remains unknown...
March 14, 2017: Virology Journal
https://www.readbyqxmd.com/read/28183350/pharmacokinetic-and-pharmacodynamic-modeling-of-sarafloxacin-against-avian-pathogenic-escherichia-coli-in-muscovy-ducks
#3
Yang Yu, Yu Feng Zhou, Jian Sun, Wei Shi, Xiao Ping Liao, Ya Hong Liu
BACKGROUND: This study focused on utilizing pharmacokinetics/pharmacodynamics (PK/PD) modeling to optimize therapeutic dosage regimens of sarafloxacin against avian pathogenic Escherichia. coli O78 strain in Muscovy ducks. The ex vivo PK/PD study of sarafloxacin was conducted in Muscovy ducks after intravenous (i.v.) and oral (p.o.) administrations at a single dose of 10 mg/kg bodyweight (BW). The serum samples were analyzed by reverse phase high-performance liquid chromatography (RP-HPLC) using a fluorescence detection method...
February 10, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28034341/genetic-diversity-and-relationship-of-indian-muscovy-duck-populations
#4
Paramasivam Kameshpandian, Subhash Thomas, Muniyandi Nagarajan
Assessment of genetic diversity within and between populations is a prerequisite for sustainable utilization of domestic species. The domestic Muscovy duck (Cairina moschata) is an economically important species around the world for its unique meat taste and low-caloric content. It is one of the important domestic species in India as it ensures food security to the rural sectors. In this study, we have analyzed the genetic diversity and relationship of four Muscovy duck populations collected from different states (Assam, Mizoram, Odisha and Kerala) of India using mtDNA cytochrome b and nuclear DNA CYP2U1 genes...
December 30, 2016: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/27879145/variations-in-endothelin-receptor-b-subtype-2-ednrb2-coding-sequences-and-mrna-expression-levels-in-4-muscovy-duck-plumage-colour-phenotypes
#5
N Wu, H Qin, M Wang, Y Bian, B Dong, G Sun, W Zhao, G Chang, Q Xu, G Chen
1. Endothelin receptor B subtype 2 (EDNRB2) is a paralog of EDNRB, which encodes a 7-transmembrane G-protein coupled receptor. Previous studies reported that EDNRB was essential for melanoblast migration in mammals and ducks. 2. Muscovy ducks have different plumage colour phenotypes. Variations in EDNRB2 coding sequences (CDSs) and mRNA expression levels were investigated in 4 different Muscovy duck plumage colour phenotypes, including black, black mutant, silver and white head. 3. The EDNRB2 gene from Muscovy duck was cloned; it had a length of 6435 bp and encoded 437 amino acids...
April 2017: British Poultry Science
https://www.readbyqxmd.com/read/27807382/disseminated-yeast-order-saccharomycetales-infection-in-a-muscovy-duckling-cairina-moschata
#6
Madhu Ravi, Janet E Hill, Champika Fernando, Gary Wobeser
A Muscovy duckling was presented for necropsy due to ongoing mortalities on a farm. Microscopic examination revealed multisystemic inflammatory lesions with intralesional and intracytoplasmic yeast-like organisms. We identified these agents as yeast belonging to the Order Saccharomycetales based on sequence data obtained from the ribosomal RNA operon.
November 2016: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/27796685/genes-involved-in-the-establishment-of-hepatic-steatosis-in-muscovy-pekin-and-mule-ducks
#7
Annabelle Tavernier, Stéphane Davail, Karine Ricaud, Marie-Dominique Bernadet, Karine Gontier
Our main objectives were to determine the genes involved in the establishment of hepatic steatosis in three genotypes of palmipeds. To respond to this question, we have compared Muscovy ducks, Pekin ducks and their crossbreed the mule duck fed ad libitum or overfed. We have shown a hepatic overexpression of fatty acid synthase (FAS) and di-acyl glycerol acyl transferase 2 (DGAT2) in overfed individuals, where DGAT2 seemed to be more regulated. This increase in lipogenesis genes is associated with a decrease of lipoprotein formation in Muscovy and mule ducks, especially apolipoprotein B (ApoB) and Microsomal Triglyceride Transfer Protein (MTTP), leading to lipid accumulation in liver...
January 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/27795286/genome-sequences-of-a-novel-recombinant-duck-circovirus-in-china
#8
Wenchao Sun, Min Zheng, Huihui Cao, Huijun Lu, Xiankai Wei, Yi Pan, Hongyun Zhang, Jiaoxiu Su, Jun Li, Ningyi Jin
In this study, YN26/2013, a novel recombinant duck circovirus (DuCV), was isolated from a Muscovy duck in Yunnan Province, southern China. The whole genome of YN26/2013 consists of 1,987 nucleotides (nt), the same genomic size as that of the DuCV-2 genotype. However, YN26/2013 shares 91.5 to 94.3% nucleotide identity similarity with previously reported type I (DuCV-1) viruses. Importantly, a novel putative recombinant event between DuCV-1 and DuCV-2 was identified as occurring within the 987- to 1111-nt region of the YN26/2013 genome...
October 20, 2016: Genome Announcements
https://www.readbyqxmd.com/read/27734222/characterization-of-batai-virus-isolated-from-a-domestic-muscovy-duck-cairina-moschate
#9
Lijiao Zhang, Qingshui Zhang, Jun Wang, Na An, Yanxin Cao, Guanghua Fu, Xueying Hu, Yu Huang, Jingliang Su
Batai virus (BATV) belongs to the genus Orthobunyavirus of the family Bunyaviridae. It has been isolated from mosquitos, pigs, cattle, and humans throughout Africa, Asia, and Europe, and causes clinical signs in domestic animals and humans. Here, we report the isolation of BATV from a domestic duck flock. Genome sequence analysis revealed clustering of this isolate in the Africa-Asia lineage. The virus replicated in mosquitos and vertebrate host cells, showing different phenotypic characteristics, and showed the potential to infect mice...
February 2017: Virus Genes
https://www.readbyqxmd.com/read/27590430/identification-of-linear-b-cell-epitopes-on-goose-parvovirus-non-structural-protein
#10
Tian-Fei Yu, Bo Ma, Jun-Wei Wang
Goose parvovirus (GPV) infection can cause a highly contagious and lethal disease in goslings and muscovy ducklings which is widespread in all major goose (Anser anser) and Muscovy duck (Cairina moschata) farming countries, leading to a huge economic loss. Humoral immune responses play a major role in GPV immune protection during GPV infection. However, it is still unknown for the localization and immunological characteristics of B-cell epitopes on GPV non-structural protein (NSP). Therefore, in this study, the epitopes on the NSP of GPV were identified by means of overlapping peptides expressed in Escherichia coli in combination with Western blot...
October 15, 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27550364/emergence-of-ndm-5-and-mcr-1-producing-escherichia-coli-clones-st648-and-st156-from-a-single-muscovy-duck-cairina-moschata
#11
Run-Shi Yang, Youjun Feng, Xiao-Yue Lv, Jia-Hong Duan, Jing Chen, Liang-Xing Fang, Jing Xia, Xiao-Ping Liao, Jian Sun, Ya-Hong Liu
Two Escherichia coli clones (sequence type 648 [ST648] and ST156) that coproduce NDM-5 and MCR-1 were detected from a single fowl in China. The blaNDM-5 gene was found on the two indistinguishable IncX3 plasmids from the two different E. coli isolates, whereas the mcr-1 gene was located on IncHI2 and IncI2 plasmids, respectively, suggesting that blaNDM-5 and mcr-1 have spread in avian intestinal flora. Also, the two strains harbor blaTEM-1, blaCTX-M-55, fosA3, and aac(6')-Ib The multiresistant E. coli strains (especially the epidemic clone ST648) might raise a potential threat to human health via food chain transmission...
November 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27527783/genetic-and-antigenic-characterization-of-h5-h6-and-h9-avian-influenza-viruses-circulating-in-live-bird-markets-with-intervention-in-the-center-part-of-vietnam
#12
Duc-Huy Chu, Masatoshi Okamatsu, Keita Matsuno, Takahiro Hiono, Kohei Ogasawara, Lam Thanh Nguyen, Long Van Nguyen, Tien Ngoc Nguyen, Thuy Thu Nguyen, Dong Van Pham, Dang Hoang Nguyen, Tho Dang Nguyen, Thanh Long To, Hung Van Nguyen, Hiroshi Kida, Yoshihiro Sakoda
A total of 3,045 environmental samples and oropharyngeal and cloacal swabs from apparently healthy poultry have been collected at three live bird markets (LBMs) at which practices were applied to reduce avian influenza (AI) virus transmission (intervention LBMs) and six conventional LBMs (non-intervention LBMs) in Thua Thien Hue province in 2014 to evaluate the efficacy of the intervention LBMs. The 178 AI viruses, including H3 (19 viruses), H4 (2), H5 (8), H6 (30), H9 (114), and H11 (5), were isolated from domestic ducks, muscovy ducks, chickens, and the environment...
August 30, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27435338/a-one-step-duplex-rrt-pcr-assay-for-the-simultaneous-detection-of-duck-hepatitis-a-virus-genotypes-1-and-3
#13
Qin Hu, Dekang Zhu, Guangpeng Ma, Anchun Cheng, Mingshu Wang, Shun Chen, Renyong Jia, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xiaoyue Chen
Duck hepatitis A virus (DHAV) is a highly infectious pathogen that causes significant bleeding lesions in the viscera of ducklings less than 3 weeks old. There are three serotypes of DHAV: serotype 1 (DHAV-1), serotype 2 (DHAV-2) and serotype 3 (DHAV-3). These serotypes have no cross-antigenicity with each other. To establish an rRT-PCR assay for the rapid detection of a mixed infection of DHAV-1 and DHAV-3, two pairs of primers and a pair of matching TaqMan probes were designed based on conserved regions of DHAV-1 VP0 and DHAV-3 VP3...
October 2016: Journal of Virological Methods
https://www.readbyqxmd.com/read/27396165/-the-isolation-and-identification-of-infectious-bronchitis-virus-ptfy-strain-in-muscovy-ducks
#14
Xiaoping Wu, Shulei Pan, Wuduo Zhou, Yijiang Wu, Yifan Huang, Baocheng Wu
In July 2009, some farms of breeding Muscovy ducks on the peak of egg laying suffered the decrease of hatching rate and the quality of the eggs showing low mortality and no evident respiratory symptoms. The swelling and congestive ovary was visible after autopsy. This study was brought out for the diagnosis of these cases. The virus was isolated and identified by the methods of virus culture in chicken embryo, physical and chemical properties test, hemagglutinin test, NDV (Newcastle diseases Virus) interference test, electron microscope observation, pathogenicity test and the gene sequence analysis...
March 2016: Bing du Xue Bao, Chinese Journal of Virology
https://www.readbyqxmd.com/read/27354303/pathogenicity-and-genetic-characterization-of-a-duck-tembusu-virus-associated-with-egg-dropping-in-muscovy-ducks
#15
Han-Qin Shen, Wen-Cheng Lin, Zhan-Xin Wang, Kai Zhang, Zhuan-Qiang Yan, Qing-Feng Zhou, Jian-Ping Qin, Qing-Mei Xie, Ying-Zuo Bi, Feng Chen
Duck Tembusu virus (DTMUV) has spread to the major duck-farming region in China, causing acute egg-production drop in Chinese duck population. In this study, we characterized a DTMUV strain (named GD2014) isolated from an egg-production drop duck farm in Guangdong province, South China. The virus was pathogenic to Muscovy duck embryos and caused severe egg production drop for laying Muscovy ducks. The genome sequence of GD2014 shared 97-99% homologies with other waterfowl-origin Tembusu viruses, and shared 89% identities with MM1775 strain isolated from mosquito...
September 2, 2016: Virus Research
https://www.readbyqxmd.com/read/27344160/analysis-of-the-genome-sequence-of-the-pathogenic-muscovy-duck-parvovirus-strain-yy-reveals-a-14-nucleotide-pair-deletion-in-the-inverted-terminal-repeats
#16
Jianye Wang, Yu Huang, Mingxu Zhou, Guoqiang Zhu
Genomic information about Muscovy duck parvovirus is still limited. In this study, the genome of the pathogenic MDPV strain YY was sequenced. The full-length genome of YY is 5075 nucleotides (nt) long, 57 nt shorter than that of strain FM. Sequence alignment indicates that the 5' and 3' inverted terminal repeats (ITR) of strain YY contain a 14-nucleotide-pair deletion in the stem of the palindromic hairpin structure in comparison to strain FM and FZ91-30. The deleted region contains one "E-box" site and one repeated motif with the sequence "TTCCGGT" or "ACCGGAA"...
September 2016: Archives of Virology
https://www.readbyqxmd.com/read/27342095/cadmium-induced-changes-in-hatchability-and-in-the-activity-of-aminotransaminases-and-selected-lysosomal-hydrolases-in-the-blood-plasma-of-muscovy-ducklings-cairina-moschata
#17
Małgorzata Dżugan, Marcin Lis
The aim of this study was to determine the effect of cadmium on Muscovy ducklings (Cairina moschata) based on hatching results and the activity of enzymes in the blood plasma. On day 6 of incubation, hatching eggs were injected into the egg albumen with 50 μl of saline solution containing Cd ions (CdCl2) at concentrations of 0 (control group), 1.3, 4.0, 7.5, 15.0 and 30 μg/egg, using 50 eggs per group. A gradual decrease in hatchability, from 52% in the control to 4% in the highest Cd dose group, was observed, with the LD50 calculated as 8 μg/egg...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27333975/impact-of-selection-for-residual-feed-intake-on-production-traits-and-behavior-of-mule-ducks
#18
L Drouilhet, R Monteville, C Molette, M Lague, A Cornuez, L Canario, E Ricard, H Gilbert
A divergent selection experiment of Muscovy sires based on the residual feed intake (RFI) of their male mule progeny was initiated in 2009. Using electronic feeders, the aim of this study was to establish whether 3 generations of selection for RFI had an impact on feeding behavior traits and general behavior, and to examine its effect on liver and meat quality. Eighty mule ducks, issued from 8 Muscovy drakes per line with extreme RFI, were tested in a pen equipped with 4 electronic feeders. Feeding behaviors were recorded from 3 to 7 wk after hatching under ad libitum feeding conditions...
September 1, 2016: Poultry Science
https://www.readbyqxmd.com/read/27329377/construction-and-sequencing-of-an-infectious-clone-of-the-goose-embryo-adapted-muscovy-duck-parvovirus-vaccine-strain-fz91-30
#19
Jianye Wang, Yu Huang, Mingxu Zhou, Philip R Hardwidge, Guoqiang Zhu
BACKGROUND: Muscovy duck parvovirus (MDPV) is the etiological agent of Muscovy duckling parvoviral disease, which is characterized by diarrhea, locomotive dysfunction, stunting, and death in young ducklings, and causes substantial economic losses in the Muscovy duck industry worldwide. FZ91-30 is an attenuated vaccine strain that is safe and immunogenic to ducklings, but the genomic information and molecular mechanism underlining the attenuation are not understood. METHODS: The FZ91-30 strain was propagated in 11-day-old embryonated goose eggs, and viral particles were purified from the pooled allantoic fluid by differential centrifugation and ultracentrifugation...
2016: Virology Journal
https://www.readbyqxmd.com/read/27314945/isolation-and-characterization-of-a-distinct-duck-origin-goose-parvovirus-causing-an-outbreak-of-duckling-short-beak-and-dwarfism-syndrome-in-china
#20
Shilong Chen, Shao Wang, Xiaoxia Cheng, Shifeng Xiao, Xiaoli Zhu, Fengqiang Lin, Nanyang Wu, Jinxiang Wang, Meiqing Huang, Min Zheng, Shaoying Chen, Fusong Yu
Many mule duck and Cherry Valley duck flocks in different duck-producing regions of China have shown signs of an apparently new disease designated "short beak and dwarfism syndrome" (SBDS) since 2015. The disease is characterized by dyspraxia, weight loss, a protruding tongue, and high morbidity and low mortality rates. In order to characterize the etiological agent, a virus designated SBDSV M15 was isolated from allantoic fluid of dead embryos following serial passage in duck embryos. This virus causes a cytopathic effect in duck embryo fibroblast (DEF) cells...
September 2016: Archives of Virology
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