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https://www.readbyqxmd.com/read/28339694/regional-acidosis-locally-inhibits-but-remotely-stimulates-ca2-waves-in-ventricular-myocytes
#1
Kerrie L Ford, Emma L Moorhouse, Mario Bortolozzi, Mark Richards, Pawel Swietach, Richard D Vaughan-Jones
AIMS: Spontaneous Ca2+ waves in cardiomyocytes are potentially arrhythmogenic. A powerful controller of Ca2+ waves is the cytoplasmic H+ concentration ([H+]i), which fluctuates spatially and temporally in conditions such as myocardial ischaemia/reperfusion. H+-control of Ca2+ waves is poorly understood. We have therefore investigated how [H+]i co-ordinates their initiation and frequency. METHODS AND RESULTS: Spontaneous Ca2+ waves were imaged (fluo-3) in rat isolated ventricular myocytes, subjected to modest Ca2+-overload...
February 21, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28338083/microvasculature-on-a-chip-study-of-the-endothelial-surface-layer-and-the-flow-structure-of-red-blood-cells
#2
Daria Tsvirkun, Alexei Grichine, Alain Duperray, Chaouqi Misbah, Lionel Bureau
Microvasculatures-on-a-chip, i.e. in vitro models that mimic important features of microvessel networks, have gained increasing interest in recent years. Such devices have allowed investigating pathophysiological situations involving abnormal biophysical interactions between blood cells and vessel walls. Still, a central question remains regarding the presence, in such biomimetic systems, of the endothelial glycocalyx. The latter is a glycosaminoglycans-rich surface layer exposed to blood flow, which plays a crucial role in regulating the interactions between circulating cells and the endothelium...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28336609/transition-from-turbulent-to-coherent-flows-in-confined-three-dimensional-active-fluids
#3
Kun-Ta Wu, Jean Bernard Hishamunda, Daniel T N Chen, Stephen J DeCamp, Ya-Wen Chang, Alberto Fernández-Nieves, Seth Fraden, Zvonimir Dogic
Transport of fluid through a pipe is essential for the operation of macroscale machines and microfluidic devices. Conventional fluids only flow in response to external pressure. We demonstrate that an active isotropic fluid, composed of microtubules and molecular motors, autonomously flows through meter-long three-dimensional channels. We establish control over the magnitude, velocity profile, and direction of the self-organized flows and correlate these to the structure of the extensile microtubule bundles...
March 24, 2017: Science
https://www.readbyqxmd.com/read/28336071/sensitive-protein-detection-and-quantification-in-paper-based-microfluidics-for-the-point-of-care
#4
Caitlin E Anderson, Kamal G Shah, Paul Yager
The design of appropriate diagnostic assays for the point of care requires development of suitable biosensors, detection methods, and diagnostic platforms for sensitive, quantitative detection of biological analytes. Protein targets in particular are especially challenging to detect quantitatively and sensitively due to the lack of amplification strategies akin to nucleic acid amplification. However, recent advances in transducer and biosensor design, new detection labels, and paper-based microfluidics may realize the goal of sensitive, fast, portable, and low-cost protein detection...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28335979/microfluidic-immunosensor-based-on-mesoporous-silica-platform-and-cmk-3-poly-acrylamide-co-methacrylate-of-dihydrolipoic-acid-modified-gold-electrode-for-cancer-biomarker-detection
#5
Matías Regiart, Martin A Fernández-Baldo, Jhonny Villarroel-Rocha, Germán A Messina, Franco A Bertolino, Karim Sapag, Aaron T Timperman, Julio Raba
We report a hybrid glass-poly (dimethylsiloxane) microfluidic immunosensor for epidermal growth factor receptor (EGFR) determination, based on the covalent immobilization of anti-EGFR antibody (anti-EGFR) on amino-functionalized mesoporous silica (AMS) retained in the central channel of a microfluidic device. The synthetized AMS was characterized by N2 adsorption-desorption isotherm, scanning electron microscopy (SEM), energy dispersive spectrometry (EDS) and infrared spectroscopy. The cancer biomarker was quantified in human serum samples by a direct sandwich immunoassay measuring through a horseradish peroxidase-conjugated anti-EGFR...
April 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28335593/simultaneous-single-cell-analysis-of-na-k-ca2-and-mg2-in-neuron-like-pc-12-cells-in-a-microfluidic-system
#6
Lu Li, Yuanyuan Fan, Qingling Li, Renjie Sheng, Haibin Si, Juan Fang, Lili Tong, Bo Tang
Various intracellular metal ions have closely related functional roles in the nervous system. An excess or deficiency of essential metal ions can contribute to neurodegenerative diseases. Thus, the detection of various metal ions in neurons is important for diagnosing and monitoring these diseases. In particular, single-cell analysis of multiple metal ions allows us to not only understand the cellular heterogeneity and differentiation, but also determine the actual relationships among multiple metal ions in each individual cell...
March 24, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28334623/rapid-on-chip-apoptosis-assay-on-human-carcinoma-cells-based-on-annexin-v-quantum-dot-probes
#7
Helena Montón, Mariana Medina-Sánchez, Joan Antoni Soler, Andrzej Chałupniak, Carme Nogués, Arben Merkoçi
Despite all the efforts made over years to study the cancer expression and the metastasis event, there is not a clear understanding of its origins and effective treatment. Therefore, more specialized and rapid techniques are required for studying cell behaviour under different drug-based treatments. Here we present a quantum dot signalling-based cell assay carried out in a segmental microfluidic device that allows studying the effect of anti-cancer drugs in cultured cell lines by monitoring phosphatidylserine translocation that occurs in early apoptosis...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28333641/droplet-size-aware-high-level-synthesis-for-micro-electrode-dot-array-digital-microfluidic-biochips
#8
Zipeng Li, Kelvin Yi-Tse Lai, Po-Hsien Yu, Krishnendu Chakrabarty, Tsung-Yi Ho, Chen-Yi Lee
A digital microfluidic biochip (DMFB) is an attractive technology platform for automating laboratory procedures in biochemistry. In recent years, DMFBs based on a microelectrode-dot-array (MEDA) architecture have been demonstrated. However, due to the inherent differences between today's DMFBs and MEDA, existing synthesis solutions for biochemistry mapping cannot be utilized for MEDA biochips. We present the first synthesis approach that can be used for MEDA biochips. We first present a general analytical model for droplet velocity and validate it experimentally using a fabricated MEDA biochip...
March 21, 2017: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28332571/profiling-protein-expression-in-circulating-tumour-cells-using-microfluidic-western-blotting
#9
Elly Sinkala, Elodie Sollier-Christen, Corinne Renier, Elisabet Rosàs-Canyelles, James Che, Kyra Heirich, Todd A Duncombe, Julea Vlassakis, Kevin A Yamauchi, Haiyan Huang, Stefanie S Jeffrey, Amy E Herr
Circulating tumour cells (CTCs) are rare tumour cells found in the circulatory system of certain cancer patients. The clinical and functional significance of CTCs is still under investigation. Protein profiling of CTCs would complement the recent advances in enumeration, transcriptomic and genomic characterization of these rare cells and help define their characteristics. Here we describe a microfluidic western blot for an eight-plex protein panel for individual CTCs derived from estrogen receptor-positive (ER+) breast cancer patients...
March 23, 2017: Nature Communications
https://www.readbyqxmd.com/read/28332020/temperature-effects-on-the-activity-shape-and-storage-of-platelets-from-13-lined-ground-squirrels
#10
Scott Cooper, Sarah Lloyd, Anthony Koch, Xingxing Lin, Katie Dobbs, Thomas Theisen, Matt Zuberbuehler, Kaley Bernhardt, Michael Gyorfi, Tanner Tenpas, Skyler Hying, Sarah Mortimer, Christine Lamont, Marcus Lehmann, Keith Neeves
The objective of this study is to determine how a hibernating mammal avoids the formation of blood clots under periods of low blood flow. A microfluidic vascular injury model was performed to differentiate the effects of temperature and shear rate on platelet adhesion to collagen. Human and ground squirrel whole blood was incubated at 15 or 37 °C and then passed through a microfluidic chamber over a 250-µm strip of type I fibrillar collagen at that temperature and the shear rates of 50 or 300 s(-1) to simulate torpid and aroused conditions, respectively...
March 23, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/28330865/an-automated-smartphone-based-diagnostic-assay-for-point-of-care-semen-analysis
#11
Manoj Kumar Kanakasabapathy, Magesh Sadasivam, Anupriya Singh, Collin Preston, Prudhvi Thirumalaraju, Maanasa Venkataraman, Charles L Bormann, Mohamed Shehata Draz, John C Petrozza, Hadi Shafiee
Male infertility affects up to 12% of the world's male population and is linked to various environmental and medical conditions. Manual microscope-based testing and computer-assisted semen analysis (CASA) are the current standard methods to diagnose male infertility; however, these methods are labor-intensive, expensive, and laboratory-based. Cultural and socially dominated stigma against male infertility testing hinders a large number of men from getting tested for infertility, especially in resource-limited African countries...
March 22, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28330860/squeeze-e-squeeze-e
#12
Kevin R King
Adding an electrical pulse to a microfluidic device that squeezes cells through narrow channels efficiently delivers DNA to the nucleus.
March 22, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/28330099/thermomyces-lanuginosus-lipase-catalyzed-synthesis-of-natural-flavor-esters-in-a-continuous-flow-microreactor
#13
Ahmad Mohammed Gumel, M S M Annuar
Enzymatic catalysis is considered to be among the most environmental friendly processes for the synthesis of fine chemicals. In this study, lipase from Thermomyces lanuginosus (Lecitase Ultra™) was used to catalyze the synthesis of flavor esters, i.e., methyl butanoate and methyl benzoate by esterification of the acids with methanol in a microfluidic system. Maximum reaction rates of 195 and 115 mM min(-1) corresponding to catalytic efficiencies (k cat/K M) of 0.30 and 0.24 min(-1) mM(-1) as well as yield conversion of 54 and 41 % were observed in methyl butanoate and methyl benzoate synthesis, respectively...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327746/combination-of-microfluidic-high-throughput-production-and-parameter-screening-for-efficient-shaping-of-gold-nanocubes-using-dean-flow-mixing
#14
Matthias Thiele, Andrea Knauer, Daniéll Malsch, Andrea Csáki, Thomas Henkel, J Michael Köhler, Wolfgang Fritzsche
Metal nanoparticles and their special optical properties, the so-called localized surface plasmon resonance (LSPR), facilitate many applications in various fields. Due to the strong dependency of the LSPR on particle geometry, their synthesis is a challenging and time-consuming procedure especially for non-spherical shapes. In contrast, micromixers offer new experimental approaches and therefore enable the simplification of several processes. By using a zigzag micromixer (Dean-Flow-Mixer, DFM) that induces Dean-flow secondary flow patterns, we theoretically and experimentally show the mixing efficiency...
March 22, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28326222/acupuncture-injection-combined-with-electrokinetic-injection-for-polydimethylsiloxane-microfluidic-devices
#15
Ji Won Ha
We recently reported acupuncture sample injection that leads to reproducible injection of nL-scale sample segments into a polydimethylsiloxane (PDMS) microchannel for microchip capillary electrophoresis. The advantages of the acupuncture injection in microchip capillary electrophoresis include capability of minimizing sample loss and voltage control hardware and capability of introducing sample plugs into any desired position of a microchannel. However, the challenge in the previous study was to achieve reproducible, pL-scale sample injections into PDMS microchannels...
2017: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/28326132/continuous-flow-synthesis-of-highly-functionalized-imidazo-oxadiazoles-facilitated-by-microfluidic-extraction
#16
Ananda Herath, Nicholas D P Cosford
A versatile continuous-flow synthesis of highly functionalized 1,2,4-oxadiazoles starting from carboxylic acids is reported. This process was applied to the multistep synthesis of imidazo[1,2-a]pyridin-2-yl-1,2,4-oxadiazoles, using a three reactor, multistep continuous-flow system without isolation of intermediates. This continuous-flow method was successfully combined with a single-step liquid-liquid microextraction unit to remove high boiling point polar solvents and impurities and provides the target compounds in high purity with excellent overall yields...
2017: Beilstein Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28325895/simulation-assisted-design-of-microfluidic-sample-traps-for-optimal-trapping-and-culture-of-non-adherent-single-cells-tissues-and-spheroids
#17
Nassim Rousset, Frédéric Monet, Thomas Gervais
This work focuses on modelling design and operation of "microfluidic sample traps" (MSTs). MSTs regroup a widely used class of microdevices that incorporate wells, recesses or chambers adjacent to a channel to individually trap, culture and/or release submicroliter 3D tissue samples ranging from simple cell aggregates and spheroids, to ex vivo tissue samples and other submillimetre-scale tissue models. Numerous MST designs employing various trapping mechanisms have been proposed in the literature, spurring the development of 3D tissue models for drug discovery and personalized medicine...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28324958/ultrafast-oscillatory-flow-pcr-for-amplication-of-urine-short-tandem-repeats
#18
Haoyu Tang, Tao Dong
The short tandem repeats (STRs) in human urine which is the most sensitive body fluid can be serviced as biomarkers for the individual identification. PCR microchips are automated, sensitive and rapid. In this report, COMSOL with Finite Element Analysis (FEA) is employed to study the temperature distribution of the base layer to optimize the structure of the microfluidic chip. Meanwhile, the oscillatory PCR microdevice is preliminary tested by DNA samples extracted from urine with the FTA papers and CSF1PO, TPOX, TH01 Multiplex kits...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28324717/fabrication-of-oriented-wrinkles-on-polydopamine-polystyrene-bilayer-films
#19
Rong Wang, Yuhua Long, Tang Zhu, Jing Guo, Chao Cai, Ning Zhao, Jian Xu
Wrinkles exist widely in nature and our life. In this paper, wrinkles on polydopamine (PDA)/polystyrene (PS) bilayer films were formed by thermal annealing due to the different thermal coefficients of expansion of each layer. The factors that influenced the dimensions of wrinkles were studied. We found that oriented wrinkles could be formed if the bilayer films were patterned with micro-grooves, and the degree of the orientation depended on the thickness of the PDA and the dimensions of the grooves. Combined with the strong adhesion, biocompatibility and reactivity of PDA, the oriented wrinkles on PDA/PS patterned bilayers may find potential application in diffraction gratings, optical sensors and microfluidic devices...
March 6, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28324241/numerical-simulation-and-scaling-of-droplet-deformation-in-a-hyperbolic-flow
#20
Erfan Kadivar, Atefeh Alizadeh
Motivated by a recent experiment (C. Ulloa et al., Phys. Rev. E 89, 033004 (2014)), droplet deformation in a flat microfluidic channel having a cross intersection with two inlet channels and two outlet channels, i.e. hyperbolic flow, is numerically investigated. Employing the boundary element method (BEM), we numerically solve the Darcy equation in the two dimensions and investigate droplet motion and droplet deformation as the droplet enters the cross intersection. We numerically find that the maximum deformation of droplet depends on droplet size, capillary number, viscosity ratio and flow rate ratio of the two inlets...
March 2017: European Physical Journal. E, Soft Matter
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