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https://www.readbyqxmd.com/read/29156107/hemodynamic-force-triggers-rapid-netosis-within-sterile-thrombotic-occlusions
#1
X Yu, J Tan, S L Diamond
BACKGROUND: Neutrophil extracellular traps (NETs) are released when neutrophils encounter infectious pathogens, especially during sepsis. Additionally, NETosis occurs during venous and arterial thrombosis, disseminated intravascular coagulation, and trauma. OBJECTIVE: We tested if hemodynamic forces trigger NETosis during sterile thrombosis. METHODS: NETs were imaged with Sytox-green during microfluidic perfusion of Factor XIIa-inhibited or thrombin-inhibited human whole blood over fibrillar collagen (± tissue factor)...
November 20, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/29155953/max-mutations-in-endometrial-cancer-clinicopathologic-associations-and-recurrent-max-p-his28arg-functional-characterization
#2
Christopher J Walker, Craig M Rush, Paola Dama, Matthew J O'Hern, Casey M Cosgrove, Jessica L Gillespie, Roman A Zingarelli, Blair Smith, Maggie E Stein, David G Mutch, Reena Shakya, Chia-Wen Chang, Karuppaiyah Selvendiran, Jonathan W Song, David E Cohn, Paul J Goodfellow
Background: Genomic studies have revealed that multiple genes are mutated at varying frequency in endometrial cancer (EC); however, the relevance of many of these mutations is poorly understood. An EC-specific recurrent mutation in the MAX transcription factor p.His28Arg was recently discovered. We sought to assess the functional consequences of this hotspot mutation and determine its association with cancer-relevant phenotypes. Methods: MAX was sequenced in 509 endometrioid ECs, and associations between mutation status and clinicopathologic features were assessed...
November 15, 2017: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/29155727/microrna-based-liquid-biopsy-the-experience-of-the-plasma-mirna-signature-classifier-msc-for-lung-cancer-screening
#3
Mavis Mensah, Cristina Borzi, Carla Verri, Paola Suatoni, Davide Conte, Ugo Pastorino, Fortunato Orazio, Gabriella Sozzi, Mattia Boeri
The development of a minimally invasive test, such as liquid biopsy, for early lung cancer detection in its preclinical phase is crucial to improve the outcome of this deadly disease. MicroRNAs (miRNAs) are tissue specific, small, non-coding RNAs regulating gene expression, which may act as extracellular messengers of biological signals derived from the cross-talk between the tumor and its surrounding microenvironment. They could thus represent ideal candidates for early detection of lung cancer. In this work, a methodological workflow for the prospective validation of a circulating miRNA test using custom made microfluidic cards and quantitative Real-Time PCR in plasma samples of volunteers enrolled in a lung cancer screening trial is proposed...
October 26, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155700/phase-behavior-of-charged-vesicles-under-symmetric-and-asymmetric-solution-conditions-monitored-with-fluorescence-microscopy
#4
Bastian Kubsch, Tom Robinson, Jan Steinkühler, Rumiana Dimova
Phase-separated giant unilamellar vesicles (GUVs) exhibiting coexisting liquid-ordered and liquid-disordered domains are a common biophysical tool to investigate the lipid raft hypothesis. Numerous studies, however, neglect the impact of physiological solution conditions. On that account, the current work presents the effect of high-salinity buffer and trans-membrane solution asymmetry on liquid-liquid phase separation in charged GUVs grown from dioleylphosphatidylglycerol, egg sphingomyelin, and cholesterol...
October 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29153944/determination-of-degree-of-rbc-agglutination-for-blood-typing-using-a-small-quantity-of-blood-sample-in-a-microfluidic-system
#5
Yaw-Jen Chang, Ching-Yuan Ho, Xin-Miao Zhou, Hsiu-Rong Yen
Blood typing assay is a critical test to ensure the serological compatibility of a donor and an intended recipient prior to a blood transfusion. This paper presents a microfluidic blood typing system using a small quantity of blood sample to determine the degree of agglutination of red blood cell (RBC). Two measuring methods were proposed: impedimetric measurement and electroanalytical measurement. The charge transfer resistance in the impedimetric measurement and the power parameter in the electroanalytical measurement were used for the analysis of agglutination level...
November 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29153761/microfluidics-for-combating-antimicrobial-resistance
#6
REVIEW
Zhengzhi Liu, Niaz Banaei, Kangning Ren
The ever-growing threat of antimicrobial resistance (AMR) demands immediate countermeasures. With its novelty and enabling features including downscaled analysis, precisely controlled local environment, and enhanced speed, accuracy, and cost-efficiency, microfluidics has demonstrated potential in several key areas, including furthering our understanding of bacteria, developing better susceptibility testing tools, and overcoming obstacles in discovery and research of new antibiotics. While ample research results in the field of microfluidics are available, their transformation into practical application is still lagging far behind...
August 8, 2017: Trends in Biotechnology
https://www.readbyqxmd.com/read/29152973/epidermal-microfluidic-electrochemical-detection-system-enhanced-sweat-sampling-and-metabolite-detection
#7
Aida Martin, Jayoung Kim, Jonas F Kurniawan, Juliane R Sempionatto, Jose R Moreto, Guangda Tang, Alan C Campbell, Andrew Shin, Min Yul Lee, Xiaofeng Liu, Joseph Wang
Despite tremendous recent efforts, non-invasive sweat monitoring is still far from delivering its early analytical promise. Here, we describe a flexible epidermal microfluidic detection platform fabricated through hybridization of lithographic and screen-printed technologies for efficient sweat sampling and continuous real-time electrochemical monitoring of sweat glucose and lactate levels. This soft, skin-mounted device judiciously merges lab-on-a-chip and electrochemical detection technologies, integrated with a miniaturized flexible electronic board for real-time wireless data transmission to a smart device...
November 20, 2017: ACS Sensors
https://www.readbyqxmd.com/read/29152029/portable-detection-of-trace-metals-in-airborne-particulates-and-sediments-via-%C3%AE-pads-and-smartphone
#8
Yuan Jia, Hui Dong, Jianping Zheng, Hao Sun
Particulate matter (PM), a key indicator of air pollution by natural and anthropogenic activities, contributes to a wide spectrum of diseases that lead to a shortening of life expectancy. It has been recognized that trace metals in airborne PM are highly toxic and can be correlated with lesion in respiratory, gastrointestinal, immunological, and hematological systems. Traditional methods for trace metal assay require sophisticated instrumentations and highly trained operators in centralized laboratories. In this work, by integrating the technologies of microfluidic paper-based analytical devices, additive manufacturing, smartphone, and colorimetric sensing, we developed the first smartphone based paper microfluidic platform for portable, disposable, and quantitative measurements of cobalt (Co), copper (Cu), and iron (Fe) in ambient air and street sediments...
November 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29152027/design-considerations-for-open-well-microfluidic-platforms-for-hypoxic-cell-studies
#9
Matthew B Byrne, Matthew T Leslie, Heeral S Patel, H Rex Gaskins, Paul J A Kenis
Regions of hypoxia are common in solid tumors and are associated with enhanced malignancy, metastasis, and chemo/radio resistance. Real-time hypoxic cellular experimentation is challenging due to the constant need for oxygen control. Most microfluidic platforms developed thus far for hypoxic cell studies are burdened by complex design parameters and are difficult to use for uninitiated investigators. However, open-well microfluidic platforms enable short and long term hypoxic cell studies with an ease of use workflow...
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29152026/particle-separation-by-phase-modulated-surface-acoustic-waves
#10
Gergely Simon, Marco A B Andrade, Julien Reboud, Jose Marques-Hueso, Marc P Y Desmulliez, Jonathan M Cooper, Mathis O Riehle, Anne L Bernassau
High efficiency isolation of cells or particles from a heterogeneous mixture is a critical processing step in lab-on-a-chip devices. Acoustic techniques offer contactless and label-free manipulation, preserve viability of biological cells, and provide versatility as the applied electrical signal can be adapted to various scenarios. Conventional acoustic separation methods use time-of-flight and achieve separation up to distances of quarter wavelength with limited separation power due to slow gradients in the force...
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29152025/advances-in-microfluidic-devices-made-from-thermoplastics-used-in-cell-biology-and-analyses
#11
REVIEW
Elif Gencturk, Senol Mutlu, Kutlu O Ulgen
Silicon and glass were the main fabrication materials of microfluidic devices, however, plastics are on the rise in the past few years. Thermoplastic materials have recently been used to fabricate microfluidic platforms to perform experiments on cellular studies or environmental monitoring, with low cost disposable devices. This review describes the present state of the development and applications of microfluidic systems used in cell biology and analyses since the year 2000. Cultivation, separation/isolation, detection and analysis, and reaction studies are extensively discussed, considering only microorganisms (bacteria, yeast, fungi, zebra fish, etc...
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29152024/direct-measurement-of-the-mechanism-by-which-magnesium-specifically-modifies-the-mechanical-properties-of-dna
#12
I Montasser, A W Coleman, Y Tauran, G Perret, L Jalabert, D Collard, B J Kim, M C Tarhan
We examine the effect of physiological cations Na(+), K(+), Mg(2+), and Ca(2+) on the mechanical properties of bundles of λ-phage DNA using silicon nanotweezers (SNTs). Integrating SNTs with a microfluidic device allows us to perform titration experiments while measuring the effect in real-time. The results show that only for Mg(2+) and in particular, at the intra-nuclear concentration (100 mM), the interaction occurs.
September 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29151901/surface-acoustic-wave-devices-for-chemical-sensing-and-microfluidics-a-review-and-perspective
#13
David B Go, Masood Z Atashbar, Zeinab Ramshani, Hsueh-Chia Chang
Surface acoustic waves (SAWs), are electro-mechanical waves that form on the surface of piezoelectric crystals. Because they are easy to construct and operate, SAW devices have proven to be versatile and powerful platforms for either direct chemical sensing or for upstream microfluidic processing and sample preparation. This review summarizes recent advances in the development of SAW devices for chemical sensing and analysis. The use of SAW techniques for chemical detection in both gaseous and liquid media is discussed, as well as recent fabrication advances that are pointing the way for the next generation of SAW sensors...
2017: Analytical Methods: Advancing Methods and Applications
https://www.readbyqxmd.com/read/29149990/simultaneous-determination-of-renal-function-biomarkers-in-urine-using-a-validated-paper-based-microfluidic-analytical-device
#14
Eduardo Luiz Rossini, Maria Izabel Milani, Emanuel Carrilho, Leonardo Pezza, Helena Redigolo Pezza
In this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (μPAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone...
January 2, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29148722/acoustic-wave-driven-functionalized-particles-for-aptamer-based-target-biomolecule-separation
#15
Raheel Ahmad, Ghulam Destgeer, Muhammad Afzal, Jinsoo Park, Husnain Ahmed, Jin Ho Jung, Kwangseok Park, Tae-Sung Yoon, Hyung Jin Sung
We developed a hybrid microfluidic device that utilized acoustic waves to drive functionalized microparticles inside a continuous flow microchannel and to separate particle-conjugated target proteins from a complex fluid. The acoustofluidic device comprised of an interdigitated transducer (IDT) that produced high-frequency surface acoustic waves (SAW) in a polydimethylsiloxane (PDMS) microfluidic channel. The SAW interacted with the sample fluid flow inside the microchannel and deflected particles from their original streamlines to achieve separation...
November 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29147556/mechanism-of-biosurfactant-adsorption-to-oil-water-interfaces-from-millisecond-scale-tensiometry-measurements
#16
Lingling Kong, Kadi Liis Saar, Raphael Jacquat, Liu Hong, Aviad Levin, Hongze Gang, Ruqiang Ye, Bozhong Mu, Tuomas P J Knowles
Many biological molecules are by their nature amphiphilic and have the ability to act as surfactants, stabilizing interfaces between aqueous and immiscible oil phases. In this paper, we explore the adsorption kinetics of surfactin, a naturally occurring cyclic lipopeptide, at hexadecane/water interfaces and compare and contrast its adsorption behaviour with that of synthetic alkyl benzene sulfonate isomers, through direct measurements of changes in interfacial tension upon surfactant adsorption. We access millisecond time resolution in kinetic measurements by making use of droplet microfluidics to probe the interfacial tension of hexadecane droplets dispersed in a continuous water phase through monitoring their deformation when the droplets are exposed to shear flows in a microfluidic channel with regular corrugations...
December 6, 2017: Interface Focus
https://www.readbyqxmd.com/read/29147027/trapping-pinning-of-colloidal-microspheres-over-glass-substrate-using-surface-features
#17
Praneet Prakash, Manoj Varma
Suspensions of micro/nano particles made of Polystyrene, Poly(methyl methacrylate), Silicon dioxide etc. have been a standard model system to understand colloidal physics. These systems have proved useful insights into phenomena such as self-assembly. Colloidal model systems are also extensively used to simulate many condensed matter phenomena such as dynamics in a quenched disordered system and glass transition. A precise control of particles using optical or holographic tweezers is essential for such studies...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29146949/a-microfluidic-platform-for-the-characterisation-of-cns-active-compounds
#18
Christopher MacKerron, Graham Robertson, Michele Zagnoni, Trevor J Bushell
New in vitro technologies that assess neuronal excitability and the derived synaptic activity within a controlled microenvironment would be beneficial for the characterisation of compounds proposed to affect central nervous system (CNS) function. Here, a microfluidic system with computer controlled compound perfusion is presented that offers a novel methodology for the pharmacological profiling of CNS acting compounds based on calcium imaging readouts. Using this system, multiple applications of the excitatory amino acid glutamate (10 nM-1 mM) elicited reproducible and reversible transient increases in intracellular calcium, allowing the generation of a concentration response curve...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29144721/functionalized-solid-state-nanopore-integrated-in-a-reusable-microfluidic-device-for-a-better-stability-and-nanoparticle-detection
#19
Jean Roman, Nathalie Jarroux, Gilles Patriarche, Olivier Francais, Juan Pelta, Bruno Le Pioufle, Laurent Bacri
Electrical detection based on single nanopores is an efficient tool to detect biomolecules, particles and study their morphology. Nevertheless the surface of the solid-state supporting the nanopore should be better controlled. Moreover, nanopore should be integrated within microfluidic architecture to facilitate control fluid exchanges. We built a reusable microfluidic system integrating a decorated membrane into a fluidic circuit, rendering the drain or refill of analytes and buffers easier. This process enhances strongly ionic conductance of the nanopore and its life-time...
November 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29144515/detection-of-sepsis-in-patient-blood-samples-using-cd64-expression-in-a-microfluidic-cell-separation-device
#20
Ye Zhang, Wenjie Li, Yun Zhou, Amanda Johnson, Amanda Venable, Ahmed Hassan, John Griswold, Dimitri Pappas
A microfluidic affinity separation device was developed for the detection of sepsis in critical care patients. An affinity capture method was developed to capture cells based on changes in CD64 expression in a single, simple microfluidic chip for sepsis detection. Both sepsis patient samples and a laboratory CD64+ expression model were used to validate the microfluidic assay. Flow cytometry analysis showed that the chip cell capture had a linear relationship with CD64 expression in laboratory models. The Sepsis Chip detected an increase in upregulated neutrophil-like cells when the upregulated cell population is as low as 10% of total cells spiked into commercially available aseptic blood samples...
November 16, 2017: Analyst
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