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https://www.readbyqxmd.com/read/28817152/gradient-free-determination-of-isoelectric-points-of-proteins-on-chip
#1
Urszula Łapińska, Kadi L Saar, Emma V Yates, Therese W Herling, Thomas Müller, Pavan K Challa, Christopher M Dobson, Tuomas P J Knowles
The isoelectric point (pI) of a protein is a key characteristic that influences its overall electrostatic behaviour. The majority of conventional methods for the determination of the isoelectric point of a molecule rely on the use of spatial gradients in pH, although significant practical challenges are associated with such techniques, notably the difficulty in generating a stable and well controlled pH gradient. Here, we introduce a gradient-free approach, exploiting a microfluidic platform which allows us to perform rapid pH change on chip and probe the electrophoretic mobility of species in a controlled field...
August 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28816436/integrated-distance-based-origami-paper-analytical-device-id-opad-for-one-step-visualized-analysis
#2
Tian Tian, Yuan An, Yiping Wu, Yanling Song, Zhi Zhu, Chaoyong James Yang
An integrated distance-based origami paper analytical device (ID-oPAD) is developed for simple, user-friendly and visual detection of targets of interest. The platform enables complete integration of target recognition, signal amplification and visual signal output, based on aptamer/invertase functionalized sepharose beads, cascaded enzymatic reactions and a 3D microfluidic paper-based analytical device with distance-based readout, respectively. The invertase-DNA conjugate is released upon target addition, after which it permeates through the cellulose and flows down into the bottom detection zone, while sepharose beads with larger size are excluded and stay in the upper zone...
August 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28816432/an-integrated-microsystem-for-real-time-detection-and-threshold-activated-treatment-of-bacterial-biofilms
#3
Sowmya Subramanian, Ekaterina I Tolstaya, Thomas Winkler, William E Bentley, Reza Ghodssi
Bacterial biofilms are the primary cause of infections in medical implants and catheters. Delayed detection of biofilm infections contributes to the widespread use of high doses of antibiotics, leading to the emergence of antibiotic-resistant bacterial strains. Accordingly, there is an urgent need for systems that can rapidly detect and treat biofilm infections in situ. As a step towards this goal, in this work we have developed for the first time a threshold-activated feedback-based impedance sensor-treatment system for combined real-time detection and treatment of biofilms...
August 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28816309/reply-to-the-comment-on-towards-a-personalized-approach-to-aromatase-inhibitor-therapy-a-digital-microfluidic-platform-for-rapid-analysis-of-estradiol-in-core-needle-biopsies-by-p-e-l%C3%A3-nning-lab-chip-2017-17-doi-10-1039-c7lc00617a
#4
Robert F Casper, Aaron R Wheeler
This article provides our response to a comment on our article that appeared in Lab on a Chip (S. Abdulwahab, A. H. C. Ng, M. D. Chamberlain, H. Ahmado, L.-A. Behan, H. Gomaa, R. F. Casper and A. R. Wheeler, Lab Chip, 2017, 17, 1594).
August 17, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28816306/comment-on-towards-a-personalized-approach-to-aromatase-inhibitor-therapy-a-digital-microfluidic-platform-for-rapid-analysis-of-estradiol-in-core-needle-biopsies-by-s-abdulwahab-a-h-c-ng-m-d-chamberlain-h-ahmado-l-a-behan-h-gomaa-r-f-casper-and-a-r-wheeler
#5
Per E Lønning
This comment on an article that appeared in Lab on a Chip (Abdulwahab et al., Lab Chip, 2017, 17, 1594) highlights the need for further validation of the proposed method.
August 17, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28816102/hydrodenticity-to-enhance-relaxivity-of-gadolinium-dtpa-within-crosslinked-hyaluronic-acid-nanoparticles
#6
Maria Russo, Alfonso Maria Ponsiglione, Ernesto Forte, Paolo Antonio Netti, Enza Torino
AIM: The efficacy of gadolinium (Gd) chelates as contrast agents for magnetic resonance imaging remains limited owing to poor relaxivity and toxic effects. Here, the effect of the hydration of the hydrogel structure on the relaxometric properties of Gd-DTPA is explained for the first time and called Hydrodenticity. RESULTS: The ability to tune the hydrogel structure is proved through a microfluidic flow-focusing approach able to produce crosslinked hyaluronic acid nanoparticles, analyzed regarding the crosslink density and mesh size, and connected to the characteristic correlation times of the Gd-DTPA...
August 17, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28816051/lipid-stabilized-water-oil-interfaces-studied-by-micro-focusing-small-angle-x-ray-scattering
#7
Emanuela Di Cola, Kristian Torbensen, Ilaria Clemente, Federico Rossi, Sandra Ristori, Ali Abou-Hassan
Water in oil (w/o) simple emulsions are dispersed micro-confined systems which find applications in many areas of advanced materials and biotechnology, such as food industry, drug delivery and cosmetics, to name but a few. In these systems, the structural and chemical properties of the boundary layer at the w/o interface are of paramount importance to determine functionality and stability. Recently, microfluidic methods have emerged as a valuable tool to fabricate monodisperse emulsion droplets. Due to the intrinsic flexibility of microfluidics, different interfaces can be obtained, and general principles governing the interfacial stability are needed to guide the experimental approach...
August 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28815723/rapid-automatic-creation-of-monodisperse-emulsion-droplets-by-microfluidic-device-with-degassed-pdms-slab-as-a-detachable-suction-actuator
#8
Yuki Murata, Yuta Nakashoji, Masaki Kondo, Yugo Tanaka, Masahiko Hashimoto
We previously developed a technique that enabled automatic creation of monodisperse water-in-oil droplets with the use of an air-evacuated PDMS microfluidic device. Although the device generated droplets over a long-time period, the production rate was slow (∼10 droplets per second). In the current study, we aimed to improve this rate, using the same fluid pumping principle described in our previous work, by remodeling our device configuration. To achieve this aim, we developed a new device with a much larger PDMS surface area-to-volume ratio within the air-trapping void space (178 cm(-1) ), than that of our earlier device (5...
August 16, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28815231/fluorescence-activated-cell-sorting-via-a-focused-traveling-surface-acoustic-beam
#9
Zhichao Ma, Yinning Zhou, David J Collins, Ye Ai
Fluorescence activated cell sorting (FACS) has become an essential technique widely exploited in biological studies and clinical applications. However, current FACS systems are quite complex, expensive, bulky, and pose potential sample contamination and biosafety issues due to the generation of aerosols in an open environment. Microfluidic technology capable of precise cell manipulation has great potential to reinvent and miniaturize conventional FACS systems. In this work, we demonstrate a benchtop scale FACS system that makes use of a highly focused traveling surface acoustic wave beam to sort out micron-sized particles and biological cells upon fluorescence interrogation at ∼kHz rates...
August 17, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28815228/inertio-elastic-flow-instabilities-in-a-90%C3%A2-bent-microchannel
#10
Junghee Kim, Sun Ok Hong, Tae Soup Shim, Ju Min Kim
Biological samples having viscoelastic properties are frequently tested using microfluidic devices. In addition, viscoelastic fluids such as polymer solutions have been used as a suspending medium to spatially focus particles in microchannels. The occurrence of flow instability even at low Reynolds number is a unique property of viscoelastic fluids. In this study, we report the instability in viscoelastic flow for a channel having a 90° bent geometry, which is a characteristic of many microfluidic devices...
August 17, 2017: Soft Matter
https://www.readbyqxmd.com/read/28815227/microparticle-parking-and-isolation-for-highly-sensitive-microrna-detection
#11
Jae Jung Kim, Lynna Chen, Patrick S Doyle
Isolating small objects, such as particles, cells, and molecules, in individual aqueous droplets is useful for chemical and biological assays. We have developed a simple microfluidic platform to immobilize (park) microparticles at defined locations, and isolate particles in monodisperse droplets surrounded by immiscible oil. While conventional methods can only achieve stochastic encapsulation of objects within larger droplets, our in situ method ensures that a single particle is entrapped in a similar-sized droplet, with ∼95% yield for parking and isolation...
August 17, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28814137/pegylated-crosslinked-hyaluronic-acid-nanoparticles-designed-through-a-microfluidic-platform-for-nanomedicine
#12
Maria Russo, Anna Maria Grimaldi, Paolo Bevilacqua, Olimpia Tammaro, Paolo Antonio Netti, Enza Torino
AIM: A high versatile microfluidic platform is proposed to design, in a one-step strategy, PEGylated crosslinked hyaluronic acid nanoparticles (cHANPs) entrapping a magnetic resonance imaging contrast agent and a dye for multimodal imaging applications. MATERIALS & METHODS: Clinically relevant biomaterials were shaped in the form of spherical NPs through a microfluidic flow focusing approach. A comparison between post processing and simultaneous PEGylation is reported to evaluate the potentiality of the chemical decoration of the cHANPs in microfluidics...
August 17, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28813598/functional-microcapsules-with-hybrid-shells-made-via-sol-gel-reaction-within-double-emulsions
#13
David G Moore, Jonathan V A Brignoli, Patrick Alberto Rühs, André R Studart
Microcapsules with organic-inorganic hybrid shells can be used as functionally-responsive delivery systems that are attractive for a broad range of applications. Hybrid-shell microcapsules have often been synthesized by the assembly of solid inorganic nanoparticles and polymers. Efforts to extend this approach to microfluidic emulsification have been hampered by problems with clogging and flow instabilities when utilizing dispersions of solid particles. In this work, hybrid shell microcapsules are synthesized through the reaction of liquid precursors, eliminating the use of solid dispersions...
August 16, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28813186/multi-normalization-and-interpolation-protocol-to-improve-norovirus-immunoagglutination-assay-from-paper-microfluidics-with-smartphone-detection
#14
Soohee Cho, Tu San Park, Kelly A Reynolds, Jeong-Yeol Yoon
Norovirus (NoV) is one of the leading causes of acute gastroenteritis, affecting 685 million people per year around the world. The best preventive measure is to screen water for possible NoV contamination, not from infected humans, preferably using rapid and field-deployable diagnostic methods. While enzyme immunoassays (EIAs) can be used for such detection, the low infectious dose as well as the generally inferior sensitivity and low titer of available NoV antibodies render critical challenges in using EIAs toward NoV detection...
August 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28813055/multivesicular-droplets-a-cell-model-system-to-study-compartmentalised-biochemical-reactions
#15
N Nuti, P E Verboket, P S Dittrich
Multivesicular vesicles (MVVs) are artificial liposomal structures widely used as a platform to study the compartmentalisation of cells and as a scaffold for artificial cell/protocell models. Current preparation techniques for MVVs, however, offer poor control on the size, lamellarity, and loading of inner lipid vesicles. Here, we introduce a microfluidic device for the production of multivesicular droplets (MVDs): a novel model system combining the ease of use and control of droplet microfluidics with the biological relevance of MVVs...
August 16, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28813001/dual-band-band-pass-filter-with-fixed-low-band-and-fluidically-tunable-high-band
#16
Eiyong Park, Daecheon Lim, Sungjoon Lim
In this work, we present a dual-band band-pass filter with fixed low-band resonant frequency and tunable high-band resonant frequency. The proposed filter consists of two split-ring resonators (SRRs) with a stub and microfluidic channels. The lower resonant frequency is determined by the length of the SRR alone, whereas the higher resonant frequency is determined by the lengths of the SRR and the stub. Using this characteristic, we fix the lower resonant frequency by fixing the SRR length and tune the higher resonant frequency by controlling the stub length by injecting liquid metal in the microfluidic channel...
August 16, 2017: Sensors
https://www.readbyqxmd.com/read/28812983/a-microfluidic-platform-for-modeling-metastatic-cancer-cell-matrix-invasion
#17
Laura Blaha, Chentian Zhang, Mario Cabodi, Joyce Y Wong
Invasion of the extracellular matrix is a critical step in the colonization of metastatic tumors. The invasion process is thought to be driven by both chemokine signaling and interactions between invading cancer cells and physical components of the metastatic niche, including endothelial cells that line capillary walls and serve as a barrier to both diffusion and invasion of the underlying tissue. Transwell chambers, a tool for generating artificial chemokine gradients to induce cell migration, have facilitated recent work to investigate the chemokine contributions to matrix invasion...
August 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28812752/extending-charge-separation-lifetime-and-distance-in-patterned-dye-sensitized-sno2-tio2-%C3%AE-m-thin-films
#18
Valeria Saavedra Becerril, Elin Sundin, Mokhtar Mapar, Maria Abrahamsson
A simple method for the preparation of patterned dye-sensitized SnO2-TiO2 thin films, designed to prolong the lifetime of the interfacial charge separated state is presented. Using microfluidic technology, the thin films were sensitized with the organic sensitizer D35 such that they contain SnO2-TiO2 areas with dye and SnO2 dye-free areas at which injected electrons can be accumulated. Single wavelength transient absorption spectroscopy confirmed significantly extended charge separation lifetime at the dye-semiconductor interface...
August 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28811527/atherosusceptible-shear-stress-activates-endoplasmic-reticulum-stress-to-promote-endothelial-inflammation
#19
Keith A Bailey, Fawaz G Haj, Scott I Simon, Anthony G Passerini
Atherosclerosis impacts arteries where disturbed blood flow renders the endothelium susceptible to inflammation. Cytokine activation of endothelial cells (EC) upregulates VCAM-1 receptors that target monocyte recruitment to atherosusceptible regions. Endoplasmic reticulum (ER) stress elicits EC dysregulation in metabolic syndrome. We hypothesized that ER plays a central role in mechanosensing of atherosusceptible shear stress (SS) by signaling enhanced inflammation. Aortic EC were stimulated with low-dose TNFα (0...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28811479/membrane-free-culture-and-real-time-barrier-integrity-assessment-of-perfused-intestinal-epithelium-tubes
#20
Sebastiaan J Trietsch, Elena Naumovska, Dorota Kurek, Meily C Setyawati, Marianne K Vormann, Karlijn J Wilschut, Henriëtte L Lanz, Arnaud Nicolas, Chee Ping Ng, Jos Joore, Stefan Kustermann, Adrian Roth, Thomas Hankemeier, Annie Moisan, Paul Vulto
In vitro models that better reflect in vivo epithelial barrier (patho-)physiology are urgently required to predict adverse drug effects. Here we introduce extracellular matrix-supported intestinal tubules in perfused microfluidic devices, exhibiting tissue polarization and transporter expression. Forty leak-tight tubules are cultured in parallel on a single plate and their response to pharmacological stimuli is recorded over 125 h using automated imaging techniques. A study comprising 357 gut tubes is performed, of which 93% are leak tight before exposure...
August 15, 2017: Nature Communications
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