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Ricardo J Flores, Aaron J Kelly, Yiting Li, Manjula Nakka, Donald A Barkauskas, Mark Krailo, Lisa L Wang, Laszlo Perlaky, Ching C Lau, M John Hicks, Tsz-Kwong Man
BACKGROUND: Osteosarcoma (OS) is the most common malignant pediatric bone tumor. The identification of novel biomarkers for early prognostication will facilitate risk-based stratification and therapy. This study investigated the significance of circulating cytokines/chemokines for predicting the prognosis at the initial diagnosis. METHODS: Luminex assays were used to measure cytokine/chemokine concentrations in blood samples from a discovery cohort of OS patients from Texas Children's Hospital (n = 37) and an independent validation cohort obtained from the Children's Oncology Group (n = 233)...
August 16, 2016: Cancer
Gaetane Nocturne, Xavier Mariette
Primary Sjögren Syndrome (pSS) is an autoimmune disease associated with an increased risk of lymphoma. Lymphomas complicating pSS are mostly low-grade B cell non-Hodgkin lymphomas, predominantly of marginal zone histological type. Mucosal localization is predominant, notably mucosa-associated lymphoid tissue lymphomas. Lymphomas often develop in organs where pSS is active, such as salivary glands. Germinal centre (GC)-like structures, high TNFSF13B (BAFF) and Flt3-ligand (FLT3LG) levels and genetic impairment of TNFAIP3 are new predictors of lymphoma development...
February 2015: British Journal of Haematology
Dokyoon Kim, Francesco Marchetti, Zuxiong Chen, Sasa Zaric, Robert J Wilson, Drew A Hall, Richard S Gaster, Jung-Rok Lee, Junyi Wang, Sebastian J Osterfeld, Heng Yu, Robert M White, William F Blakely, Leif E Peterson, Sandhya Bhatnagar, Brandon Mannion, Serena Tseng, Kristen Roth, Matthew Coleman, Antoine M Snijders, Andrew J Wyrobek, Shan X Wang
Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7...
2013: Scientific Reports
Heiner Falkenberg, Teja Falk Radke, Gesine Kögler, Kai Stühler
Ex vivo expansion of haematopoetic cells by application of specific cytokines is one approach to overcome boundaries in cord blood transplantation due to limited numbers of haematopoetic stem cells. While many protocols describe an effective increase of total cell numbers and the amount of CD34-positive cells, it still remains unclear if and how the procedure actually affects the cells' properties. In the presented publications, CD34-positive cells were isolated from cord blood and expanded for up to 7 days in media supplemented with stem cell factor (SCF), thrombopoietin (THPO), interleukin 6 (IL-6), and fms-related tyrosine kinase 3 ligand (FLT3lg)...
2013: Stem Cells International
Romain Barbet, Isabelle Peiffer, Antoinette Hatzfeld, Pierre Charbord, Jacques A Hatzfeld
We present a strategy to identify developmental/differentiation and plasma membrane marker genes of the most primitive human Mesenchymal Stem Cells (hMSCs). Using sensitive and quantitative TaqMan Low Density Arrays (TLDA) methodology, we compared the expression of 381 genes in human Embryonic Stem Cells (hESCs), hESC-derived MSCs (hES-MSCs), and hMSCs. Analysis of differentiation genes indicated that hES-MSCs express the sarcomeric muscle lineage in addition to the classical mesenchymal lineages, suggesting they are more primitive than hMSCs...
2011: Stem Cells International
Masashi Sanada, Takahiro Suzuki, Lee-Yung Shih, Makoto Otsu, Motohiro Kato, Satoshi Yamazaki, Azusa Tamura, Hiroaki Honda, Mamiko Sakata-Yanagimoto, Keiki Kumano, Hideaki Oda, Tetsuya Yamagata, Junko Takita, Noriko Gotoh, Kumi Nakazaki, Norihiko Kawamata, Masafumi Onodera, Masaharu Nobuyoshi, Yasuhide Hayashi, Hiroshi Harada, Mineo Kurokawa, Shigeru Chiba, Hiraku Mori, Keiya Ozawa, Mitsuhiro Omine, Hisamaru Hirai, Hiromitsu Nakauchi, H Phillip Koeffler, Seishi Ogawa
Acquired uniparental disomy (aUPD) is a common feature of cancer genomes, leading to loss of heterozygosity. aUPD is associated not only with loss-of-function mutations of tumour suppressor genes, but also with gain-of-function mutations of proto-oncogenes. Here we show unique gain-of-function mutations of the C-CBL (also known as CBL) tumour suppressor that are tightly associated with aUPD of the 11q arm in myeloid neoplasms showing myeloproliferative features. The C-CBL proto-oncogene, a cellular homologue of v-Cbl, encodes an E3 ubiquitin ligase and negatively regulates signal transduction of tyrosine kinases...
August 13, 2009: Nature
Jianbiao Zhou, Chonglei Bi, Jasinghe V Janakakumara, Shaw-Cheng Liu, Wee-Joo Chng, Kian-Ghee Tay, Lai-Fong Poon, Zhigang Xie, Senthilnathan Palaniyandi, Hanry Yu, Keith B Glaser, Daniel H Albert, Steven K Davidsen, Chien-Shing Chen
To further investigate potential mechanisms of resistance to FLT3 inhibitors, we developed a resistant cell line by long-term culture of MV4-11 cells with ABT-869, designated as MV4-11-R. Gene profiling reveals up-regulation of FLT3LG (FLT3 ligand) and BIRC5 (survivin), but down-regulation of SOCS1, SOCS2, and SOCS3 in MV4-11-R cells. Hypermethylation of these SOCS genes leads to their transcriptional silencing. Survivin is directly regulated by STAT3. Stimulation of the parental MV4-11 cells with FLT3 ligand increases the expression of survivin and phosphorylated protein STAT1, STAT3, STAT5...
April 23, 2009: Blood
Ikuo Kashiwakura, Osamu Inanami, Miho Murakami, Tsuneo A Takahashi, Mikinori Kuwabara, Yoshinari Takagi
Combinations of thrombopoietin and cytokines that act on megakaryocyte development (stem cell factor, IL3, IL6, IL11, flt3 ligand (now known as FLT3LG), erythropoietin, GM-CSF and G-CSF were evaluated for their ability to enhance clonal growth in vitro of X-irradiated CD34(+) megakaryocytic progenitor cells (CFU-megakaryocytes) purified from normal human peripheral blood. These data were compared with corresponding results described previously for CD34(+) CFU-megakaryocytes from human placental/umbilical cord blood (I...
August 2002: Radiation Research
I Kashiwakura, M Kuwabara, O Inanami, M Murakami, Y Hayase, T A Takahashi, Y Takagi
The in vitro radiation sensitivity of CFU-Meg isolated from human placental and umbilical cord blood was evaluated in plasma clot cultures stimulated by recombinant human cytokines, including thrombopoietin, the FLT3 ligand (FLT3LG), interleukin-3, interleukin-11 and stem cell factor. The CD34(+) cells were irradiated with X rays at a dose rate of 73 cGy/ min. The megakaryocyte colonies were identified by using an FITC-conjugated antibody to glycoprotein IIbIIIa and were classified into two groups based on colony size: large colonies (immature CFU-Meg) and small colonies (mature CFU-Meg)...
February 2000: Radiation Research
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