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Olfactory ensheathing cells

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https://www.readbyqxmd.com/read/29737535/neural-differentiation-of-human-adipose-derived-mesenchymal-stem-cells-induced-by-glial-cell-conditioned-media
#1
Debora Lo Furno, Giuliana Mannino, Rosario Giuffrida, Elisa Gili, Carlo Vancheri, Maria S Tarico, Rosario E Perrotta, Rosalia Pellitteri
Adipose-derived mesenchymal stem cells (ASCs) may transdifferentiate into cells belonging to mesodermal, endodermal, and ectodermal lineages. The aim of this study was to verify whether a neural differentiation of ASCs could be induced by a conditioned medium (CM) obtained from cultures of olfactory ensheathing cells (OECs) or Schwann cells (SCs). ASCs were isolated from the stromal vascular fraction of adipose tissue and expanded for 2-3 passages. They were then cultured in OEC-CM or SC-CM for 24 hr or 7 days...
May 8, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29713644/the-therapeutic-effects-after-transplantation-of-whole-layer-olfactory-mucosa-in-rats-with-optic-nerve-injury
#2
Shun Gong, Hai Jin, Danfeng Zhang, Wei Zou, Chunhui Wang, Zhenxing Li, Rongbin Chen, Yan Dong, Lijun Hou
Background: Existing evidence suggests the potential therapy of transplanting olfactory ensheathing cells (OEC) either alone or in combination with neurotrophic factors or other cell types in optic nerve injury (ONI). However, clinical use of autologous OEC in the acute stages of ONI is not possible. On the other hand, acute application of heterologous transplantation may bring the issue of immune rejection. The olfactory mucosa (OM) with OEC in the lamina propria layer is located in the upper region of the nasal cavity and is easy to dissect under nasal endoscopy, which makes it a candidate as autograft material in acute stages of ONI...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29659152/cranial-pair-i-the-olfactory-nerve
#3
Carlos Crespo, Teresa Liberia, José Miguel Blasco-Ibáñez, Juan Nácher, Emilio Varea
The olfactory nerve constitutes the first cranial pair. Compared with other cranial nerves, it depicts some atypical features. First, the olfactory nerve does not form a unique bundle. The olfactory axons join other axons and form several small bundles or fascicles: the fila olfactoria. These fascicles leave the nasal cavity, pass through the lamina cribrosa of the ethmoid bone and enter the brain. The whole of these fascicles is what is known as the olfactory nerve. Second, the olfactory sensory neurons, whose axons integrate the olfactory nerve, connect the nasal cavity and the brain without any relay...
April 16, 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29608947/microencapsulated-schwann-cell-transplantation-inhibits-p2x2-3-receptors-overexpression-in-a-sciatic-nerve-injury-rat-model-with-neuropathic-pain
#4
Ya-Ling Zhang, Yi-Guo Liu, De-Jian Chen, Bao-Lin Yang, Tao-Tao Liu, Jia-Juan Li, Xiu-Qi Wang, Hao-Tian Rose Li, Zeng-Xu Liu
Transplantation of Schwann cells (SCs) can promote axonal regeneration and formation of the myelin sheath, reduce inflammation, and promote repair to the damaged nerve. Our previous studies have shown that transplantation of free or micro-encapsulated olfactory ensheathing cells can relieve neuropathic pain. There are no related reports regarding whether the transplantation of micro-encapsulated SCs can alleviate neuropathic pain mediated by P2X2/3 receptors. In the present study, we micro-encapsulated SCs in alginic acid and transplanted them into the region surrounding the injured sciatic nerve in the rat model of chronic constriction injury (CCI)...
March 30, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29567386/the-grueneberg-olfactory-organ-neuroepithelium-recovers-after-injury
#5
Fatemeh Chehrehasa, Angela Jacques, James A St John, Jenny A K Ekberg
The Grueneberg organ (also termed Grueneberg ganglion) is an olfactory subsystem at the rostral nasal septum of rodents, and has been suggested to exist also in humans. Grueneberg organ neurons respond to coldness and alarm pheromones, but the anatomical arrangement and regenerative capacity are not fully characterised. We examined the relationship between the glia and the neurons using crosses of two transgenic mouse lines, S100ß-DsRed and OMP-ZsGreen, to visualise olfactory ensheathing cells (OECs) and Grueneberg olfactory neurons, respectively...
March 19, 2018: Brain Research
https://www.readbyqxmd.com/read/29504110/preparation-of-human-olfactory-ensheathing-cells-for-the-therapy-of-spinal-cord-injuries
#6
А D Voronova, O V Stepanova, M P Valikhov, A V Chadin, А S Dvornikov, I V Reshetov, V P Chekhonin
We developed an optimal protocol for preparing and culturing of olfactory ensheathing cells from human olfactory mucosa. Using this protocol, we obtained a culture enriched with human olfactory ensheathing cells. Immunofluorescence analysis by simultaneous expression of GFAP and p75NTR markers showed that the content of ensheathing cells was maximum in passage 3 and 4 cultures (94 and 89.5%, respectively). The developed protocol can be recommended for obtaining autologous preparations of human ensheathing cells for cell therapy of spinal cord injuries...
March 2018: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29492839/olfactory-ensheathing-cells-promote-physiological-repair-of-injured-recurrent-laryngeal-nerves-and-functional-recovery-of-glottises-in-dogs
#7
Hongyi Liu, Yu Pu, Yaping Xu, He Xu, Huanhai Liu, Yin Cheng, Weihua Xu, Xiaoping Chen, Jingping Fan
The aim of this study was to investigate whether the transplantation of olfactory-ensheathing cells (OECs) could physiologically repair severely injured recurrent laryngeal nerve (RLN) in dogs. Adult Beagle dogs were surgically introduced with a 10-mm defect in the left RLN and transplanted with a nerve guide (NEUROLAC) containing dog olfactory mucosa-olfactory-ensheathing cells (OM-OECs) in matrigel. The effects of OM-OECs on the morphology, histology, and electrophysiology of the injured RLNs, glottis movement, and voice acoustics were comparatively studied...
February 28, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29451202/stem-cells-for-spinal-cord-injuries-bearing-translational-potential
#8
REVIEW
Kyriakos Dalamagkas, Magdalini Tsintou, Alexander M Seifalian
Spinal cord injury (SCI) is a highly debilitating neurological disease, which still lacks effective treatment strategies, causing significant financial burden and distress to the affected families. Nevertheless, nanotechnology and regenerative medicine strategies holding promise for the development of novel therapies that would reach from bench to bedside to serve the SCI patients. There has already been significant progress in the field of cell-based therapies, with the clinical application for SCI, currently in phase II of the clinical trial...
January 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/29352743/cotransplantation-of-adipose-tissue-derived-stromal-cells-and-olfactory-ensheathing-cells-for-spinal-cord-injury-repair
#9
Eduardo D Gomes, Sofia S Mendes, Rita C Assunção-Silva, Fábio G Teixeira, Ana O Pires, Sandra I Anjo, Bruno Manadas, Hugo Leite-Almeida, Jeffrey M Gimble, Nuno Sousa, Angelo C Lepore, Nuno A Silva, António J Salgado
Patients suffering from spinal cord injury (SCI) still have a dismal prognosis. Despite all the efforts developed in this area, currently there are no effective treatments. Therefore, cell therapies have been proposed as a viable alternative to the current treatments used. Adipose tissue-derived stromal cells (ASCs) and olfactory ensheathing cells (OECs) have been used with promising results in different models of SCI, namely due to the regenerative properties of the secretome of the first, and the guidance capability of the second...
January 20, 2018: Stem Cells
https://www.readbyqxmd.com/read/29336992/schwann-cell-lamellipodia-regulate-cell-cell-interactions-and-phagocytosis
#10
Johana Tello Velasquez, James A St John, Lynn Nazareth, Jenny A K Ekberg
Lamellipodia in Schwann cells (SCs) are crucial for myelination, but their other biological functions remain largely uncharacterised. Two types of lamellipodia exist in SCs: axial lamellipodia at the outermost edge of the cell processes, and radial lamellipodia appearing peripherally along the entire cell. We have previously shown that radial lamellipodia on olfactory glia (olfactory ensheathing cells; OECs) promote cell-cell adhesion, contact-mediated migration and phagocytosis. Here we have investigated whether lamellipodia in SCs have similar roles...
April 2018: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/29330870/cell-therapy-for-spinal-cord-injury-with-olfactory-ensheathing-glia-cells-oecs
#11
REVIEW
Rosa M Gómez, Magdy Y Sánchez, Maria Portela-Lomba, Kemel Ghotme, George E Barreto, Javier Sierra, M Teresa Moreno-Flores
The prospects of achieving regeneration in the central nervous system (CNS) have changed, as most recent findings indicate that several species, including humans, can produce neurons in adulthood. Studies targeting this property may be considered as potential therapeutic strategies to respond to injury or the effects of demyelinating diseases in the CNS. While CNS trauma may interrupt the axonal tracts that connect neurons with their targets, some neurons remain alive, as seen in optic nerve and spinal cord (SC) injuries (SCIs)...
January 13, 2018: Glia
https://www.readbyqxmd.com/read/29321494/transplantation-of-olfactory-ensheathing-cells-on-functional-recovery-and-neuropathic-pain-after-spinal-cord-injury-systematic-review-and-meta-analysis
#12
Babak Nakhjavan-Shahraki, Mahmoud Yousefifard, Vafa Rahimi-Movaghar, Masoud Baikpour, Farinaz Nasirinezhad, Saeed Safari, Mehdi Yaseri, Ali Moghadas Jafari, Parisa Ghelichkhani, Abbas Tafakhori, Mostafa Hosseini
There are considerable disagreements on the application of olfactory ensheathing cells (OEC) for spinal cord injury (SCI) rehabilitation. The present meta-analysis was designed to investigate the efficacy of OEC transplantation on motor function recovery and neuropathic pain alleviation in SCI animal models. Accordingly, all related studies were identified and included. Two independent researchers assessed the quality of the articles and summarized them by calculating standardized mean differences (SMD). OEC transplantation was shown to significantly improve functional recovery (SMD = 1...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29291419/neuroprotective-effect-of-olfactory-ensheathing-cells-co-transfected-with-nurr1-and-ngn2-in-both-in-vitro-and-in-vivo-models-of-parkinson-s-disease
#13
Qingqing Liu, Qi Qin, Hongxue Sun, Di Zhong, Ran An, Yushuang Tian, Hongping Chen, Jing Jin, Haining Wang, Guozhong Li
AIMS: The aim of the study is to evaluate the neuroprotective effects of olfactory ensheathing cells (OECs) with the overexpression of nuclear receptor-related factor 1 (Nurr1) and neurogenin 2 (Ngn2) in experimental models of Parkinson's disease (PD) and to elucidate the potential mechanism underlying the neuroprotective effects of OECs-Nurr1-Ngn2. MATERIALS AND METHODS: In vitro study, OECs-Nurr1-Ngn2 conditioned medium (CM) was added to MPP+ -treated PC12 cells for 24h, and then the viability of PC12 cells, oxidative stress and apoptosis were detected...
February 1, 2018: Life Sciences
https://www.readbyqxmd.com/read/29251109/human-olfactory-ensheathing-cell-transplantation-improves-motor-function-in-a-mouse-model-of-type-3-spinocerebellar-ataxia
#14
Jeanne Hsieh, Jen-Wei Liu, Horng-Jyh Harn, Kuo-Wei Hsueh, Karthyayani Rajamani, Yu-Chen Deng, Chih-Min Chia, Woei-Cheang Shyu, Shinn-Zong Lin, Tzyy-Wen Chiou
Spinocerebellar ataxia (SCA) is a progressive neurodegenerative disease that affects the cerebellum and spinal cord. Among the 40 types of SCA, SCA type 3 (SCA3), also referred to as Machado-Joseph disease, is the most common. In the present study, we investigated the therapeutic effects of intracranial transplantation of human olfactory ensheathing cells (hOECs) in the ATXN3-84Q mouse model of SCA3. Motor function begins to decline in ATXN3-84Q transgenic mice at approximately 13 weeks of age. ATXN3-84Q mice that received intracranial hOEC transplantation into the dorsal raphe nucleus of the brain exhibited significant improvements in motor function, as measured by the rotarod performance test and footprint pattern analysis...
October 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29228020/transplantation-of-canine-olfactory-ensheathing-cells-producing-chondroitinase-abc-promotes-chondroitin-sulphate-proteoglycan-digestion-and-axonal-sprouting-following-spinal-cord-injury
#15
Darren Carwardine, Jonathan Prager, Jacob Neeves, Elizabeth M Muir, James Uney, Nicolas Granger, Liang-Fong Wong
Olfactory ensheathing cell (OEC) transplantation is a promising strategy for treating spinal cord injury (SCI), as has been demonstrated in experimental SCI models and naturally occurring SCI in dogs. However, the presence of chondroitin sulphate proteoglycans within the extracellular matrix of the glial scar can inhibit efficient axonal repair and limit the therapeutic potential of OECs. Here we have used lentiviral vectors to genetically modify canine OECs to continuously deliver mammalian chondroitinase ABC at the lesion site in order to degrade the inhibitory chondroitin sulphate proteoglycans in a rodent model of spinal cord injury...
2017: PloS One
https://www.readbyqxmd.com/read/29190980/transplanted-olfactory-ensheathing-cells-restore-retinal-function-in-a-rat-model-of-light-induced-retinal-damage-by-inhibiting-oxidative-stress
#16
Langyue Xue, Yuxiao Zeng, Qiyou Li, Yijian Li, Zhengya Li, Haiwei Xu, Zhengqin Yin
There is still not an effective treatment for continuous retinal light exposure and subsequent photoreceptor degeneration. Olfactory ensheathing cell (OEC) transplantation has been shown to be neuroprotective in spinal cord, and optic nerve injury and retinitis pigmentosa. However, whether OECs protect rat photoreceptors against light-induced damage and how this may work is unclear. Thus, to elucidate this mechanism, purified rat OECs were grafted into the subretinal space of a Long-Evans rat model with light-induced photoreceptor damage...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29150616/nmr-metabolomics-for-stem-cell-type-discrimination
#17
Franca Castiglione, Monica Ferro, Evangelos Mavroudakis, Rosalia Pellitteri, Patrizia Bossolasco, Damiano Zaccheo, Massimo Morbidelli, Vincenzo Silani, Andrea Mele, Davide Moscatelli, Lidia Cova
Cell metabolism is a key determinant factor for the pluripotency and fate commitment of Stem Cells (SCs) during development, ageing, pathological onset and progression. We derived and cultured selected subpopulations of rodent fetal, postnatal, adult Neural SCs (NSCs) and postnatal glial progenitors, Olfactory Ensheathing Cells (OECs), respectively from the subventricular zone (SVZ) and the olfactory bulb (OB). Cell lysates were analyzed by proton Nuclear Magnetic Resonance (1 H-NMR) spectroscopy leading to metabolites identification and quantitation...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29094304/perineuronal-nets-labeled-by-monoclonal-antibody-vc1-1-ensheath-interneurons-expressing-parvalbumin-and-calbindin-in-the-rat-amygdala
#18
Alexander J McDonald, Patricia G Hamilton, Colin J Barnstable
Perineuronal nets (PNNs) are specialized condensations of extracellular matrix that ensheath particular neuronal subpopulations in the brain and spinal cord. PNNs regulate synaptic plasticity, including the encoding of fear memories by the amygdala. The present immunohistochemical investigation studied PNN structure and distribution, as well as the neurochemistry of their ensheathed neurons, in the rat amygdala using monoclonal antibody VC1.1, which recognizes a glucuronic acid 3-sulfate glycan associated with PNNs in the cerebral cortex...
April 2018: Brain Structure & Function
https://www.readbyqxmd.com/read/28973792/rostro-caudal-maturation-of-glial-cells-in-the-accessory-olfactory-system-during-development-involvement-in-outgrowth-of-gnrh-neurites
#19
Sarah Geller, Didier Lomet, Alain Caraty, Yves Tillet, Anne Duittoz, Pascal Vaudin
During mammalian embryonic development, GnRH neurones differentiate from the nasal placode and migrate through the nasal septum towards the forebrain. We previously showed that a category of glial cells, the olfactory ensheathing cells (OEC), forms the microenvironment of migrating GnRH neurones. Here, to characterize the quantitative and qualitative importance of this glial, we investigated the spatiotemporal maturation of glial cells in situ and the role of maturing glia in GnRH neurones development ex vivo...
October 3, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28966235/ginsenoside-rg1-promotes-the-migration-of-olfactory-ensheathing-cells-via-the-pi3k-akt-pathway-to-repair-rat-spinal-cord-injury
#20
Yin-Yao Tang, Wei-Xiao Guo, Zheng-Feng Lu, Mao-Hua Cheng, Yi-Xin Shen, Ying-Zi Zhang
The aim of this study was to determine the effects of ginsenoside Rg1 on the migration of olfactory ensheathing cells (OECs) in vitro, and its influence on the therapeutic efficacy of OECs transplanted in vivo for the treatment of spinal cord injury (SCI). Primary cultured and purified OECs (prepared from rats) were treated with ginsenoside Rg1. The wound healing test indicated that ginsenoside Rg1 promoted the migration of OECs. Real-time RT-PCR demonstrated that ginsenoside Rg1 upregulated the expression of migration-related factors of OECs, including matrix metalloproteinases-2 (MMP-2), MMP-9, and neural cell adhesion molecule 1 (NCAM1)...
2017: Biological & Pharmaceutical Bulletin
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