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e_cadherin expression of gastric cancer

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https://www.readbyqxmd.com/read/28611525/clinical-significance-of-expression-of-proliferating-cell-nuclear-antigen-and-e-cadherin-in-gastric-carcinoma
#1
Lin Hu, Hong-Lang Li, Wei-Feng Li, Jun-Min Chen, Jian-Tao Yang, Jun-Jing Gu, Lin Xin
AIM: to investigate the expression of proliferating cell nuclear antigen (PCNA) and E-cadherin in gastric carcinoma and to analyze their clinical significance. METHODS: A total of 146 patients were selected for this study, including 38 patients with intestinal metaplasia, 42 with dysplasia, and 66 with primary gastric cancer. In addition, 40 patients with normal gastric tissues were selected as controls. The expression of PCNA and E-cadherin was detected by immunohistochemistry...
May 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28601080/microrna-630-suppresses-epithelial-to-mesenchymal-transition-by-regulating-foxm1-in-gastric-cancer-cells
#2
Jing Feng, Xiaojuan Wang, Weihua Zhu, Si Chen, Changwei Feng
In the present study, we investigated the functional role of microRNA (miR)-630 in epithelial-to-mesenchymal transition (EMT) of gastric cancer (GC) cells, as well as the regulatory mechanism. Cells of human GC cell line SGC 7901 were transfected with miR-630 mimic or miR-630 inhibitor. The transfection efficiency was confirmed by qRT-PCR. Cell migration and invasion were determined by Transwell assay. Protein expression of E-cadherin, vimentin, and Forkhead box protein M1 (FoxM1) was tested by Western blot...
June 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/28600569/fpr2-promotes-invasion-and-metastasis-of-gastric-cancer-cells-and-predicts-the-prognosis-of-patients
#3
Xi-Lu Hou, Cheng-Dong Ji, Jun Tang, Yan-Xia Wang, Dong-Fang Xiang, Hai-Qing Li, Wei-Wei Liu, Jiao-Xue Wang, He-Zhong Yan, Yan Wang, Peng Zhang, You-Hong Cui, Ji-Ming Wang, Xiu-Wu Bian, Wei Liu
Formyl peptide receptor 2 (FPR2), a classical chemoattractant receptor of G-protein-coupled receptors, is reported to be involved in invasion and metastasis of some cancers, but the role of FPR2 in gastric cancer (GC) has not yet been elucidated. In this study, we found that the levels of FPR2 expression in GC were positively correlated with invasion depth, lymph node metastasis and negatively correlated with the patients' overall survival. Multivariate analysis indicated that FPR2 expression was an independent prognostic marker for GC patients...
June 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28599424/wnt10b-is-critical-for-the-progression-of-gastric-cancer
#4
Xiao-Dan Wu, Qing-Li Bie, Bin Zhang, Zi-He Yan, Zhi-Jun Han
The family of Wnt proteins have been implicated in embryogenesis by regulation of cell fate and pattern formation, and also in human carcinogenesis. Wnt10B was previously shown to be involved in breast cancer development. The present study assessed the association of Wnt10B expression in human gastric cancer tissue specimens with clinicopathological data from these patients. Wnt10B expression in the regulation of gastric cancer cell proliferation and migration capacity in vitro was then investigated. The data revealed that Wnt10B mRNA and protein were upregulated in gastric cancer tissue samples and the upregulated Wnt10B mRNA was associated with gastric cancer metastasizing to lymph nodes...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28595915/predictive-value-for-lymph-node-metastasis-of-epithelial-mesenchymal-transition-and-cancer-stem-cell-marker-expression-in-early-gastric-cancer
#5
Kyung Han Nam, Hyuk Yoon, Kyungbun Lee, Do Joong Park, Hyung-Ho Kim, Hye Seung Lee, Eun Shin
BACKGROUND: Minimally invasive therapies, including endoscopic mucosal resection or sentinel node navigation surgery, have been widely applied in early gastric cancer because of their benefits in promoting patient quality of life. However, lymph node dissection is beyond the capability of endoscopic therapy, and in sentinel node navigation surgery, the potential for skip metastasis is not negligible. Therefore, the possibility of lymph node metastasis is the most important factor to consider when deciding whether to apply the minimally invasive therapies...
March 21, 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28582847/mir-133b-inhibits-proliferation-and-invasion-of-gastric-cancer-cells-by-up-regulating-fbn1-expression
#6
Deying Yang, Deqin Zhao, Xinrui Chen
We aimed to investigate the influence of miR-133b/fibrillin 1 (FBN1) on proliferation and invasion of human gastric cancer (GC) cells. Carcinomatous and adjacent tissues of 43 GC patients, normal gastric mucosa cell line GES-1 and GC cell lines including AGS, HGC-27, KATO III, NCI-N87, SGC-7901, MKN-45 and MGC-803 were collected. Then, the expressions of miR-133b and FBN1 were detected by qRT-PCR. The dual luciferase reporter gene assay was conducted to determine the targeting relationship between miR-133b and FBN1...
May 19, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28577495/knockdown-of-long-noncoding-rna-ghet1-inhibits-cell-activation-of-gastric-cancer
#7
Hui Huang, Wenjun Liao, Xueqiang Zhu, Hao Liu, Lijun Cai
OBJECTION: The aim of this study was to evaluate the effects of lncRNA GHET1 in developing of Gastric Cancer. METHODS: Collecting the 20 gastric cancer patients and evaluated the pathological of adjacent and tumor tissues by HE stating. We analyzed the protein expression of Numb in gastric cancer (GC) tissues by using IHC and the gene expression of Numb and GHET1 in adjacent and GC tissues by RT-PCR. The AGS cells were divided into 3 groups: Control (Co), NC and shRNA groups...
August 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28550287/up-regulation-of-long-non-coding-rna-xloc_010235-regulates-epithelial-to-mesenchymal-transition-to-promote-metastasis-by-associating-with-snail1-in-gastric-cancer
#8
Yu-Yi Liu, Ze-Hong Chen, Jian-Jun Peng, Jia-Lin Wu, Yu-Jie Yuan, Er-Tao Zhai, Shi-Rong Cai, Yu-Long He, Wu Song
We previously performed long non-coding RNA (lncRNA) expression microarray analyses to identify novel indicators for gastric cancer (GC) metastasis and prognosis in which we identified lncRNA XLOC_010235 (XLOC) as a candidate RNA. However, XLOC_010235 molecular mechanism of action remains unclear. Gain and loss of function approaches were used to investigate the biological role of XLOC in vitro. The effects of XLOC on cell viability were assessed by CCK-8 proliferation assays. Real-time PCR, western-blot and immunofluorescence were used to evaluate the mRNA and protein expression of Snail and multiple EMT related molecules...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28540637/importance-of-human-peritoneal-mesothelial-cells-in-the-progression-fibrosis-and-control-of-gastric-cancer-inhibition-of-growth-and-fibrosis-by-tranilast
#9
Hiroto Saito, Sachio Fushida, Shinichi Harada, Tomoharu Miyashita, Katsunobu Oyama, Takahisa Yamaguchi, Tomoya Tsukada, Jun Kinoshita, Hidehiro Tajima, Itasu Ninomiya, Tetsuo Ohta
BACKGROUND: Scirrhous gastric cancer is an intractable disease with a high incidence of peritoneal dissemination and obstructive symptoms (e.g., ileus, jaundice, and hydronephrosis) arising from accompanying marked fibrosis. Microenvironmental interactions between cancer cells and cancer-associated fibroblasts are the suggested cause of the disease. We elucidated the mechanisms of tumor growth and fibrosis using human peritoneal mesothelial cells (HPMCs) and investigated the effects of tranilast treatment on cells and a xenograft mouse model of fibrosis...
May 24, 2017: Gastric Cancer
https://www.readbyqxmd.com/read/28539961/yiqi-huayu-jiedu-decoction-inhibits-the-invasion-and-metastasis-of-gastric-cancer-cells-through-tgf-%C3%AE-smad-pathway
#10
Ting-Ting Wu, Jun Lu, Pei-Qiu Zheng, Shen-Lin Liu, Jian Wu, Wei Sun, Qing-Min Sun, Nai-Xia Ma, Xue-Lian Ding, Min Chen, Xi Zou
Background. Yiqi Huayu Jiedu Decoction (YHJD) can obviously improve the quality of life of those patients with gastric cancer and prolong their survival. Methods. In vitro experiments, we observe YHJD's effect on the cells' proliferation by MTT assay. Cell adhesion assay, wound-healing assay, and Transwell invasion assay serve to detect its influence on cells' adhesion, migration, and invasion, respectively. Inhibitor (10 μM/L of SB431542) and activator (10 ng/mL of TGF-β) of TGF-β/Smad pathway were used to estimate whether YHJD's impact on the biological behavior of gastric cancer cells was related to TGF-β/Smad pathway...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28534934/overexpression-of-stromal-interaction-molecule-1-may-promote-epithelial%C3%A2-mesenchymal-transition-and-indicate-poor-prognosis-in-gastric-cancer
#11
Guobin Wu, Yong Li, Bibo Tan, Liqiao Fan, Qun Zhao, Yü Liu, Zhidong Zhang
The aim of the present study was to investigate the prognostic significance of stromal interaction molecule 1 (STIM1) expression in gastric cancer (GC) and examine the association between STIM1 and epithelial‑mesenchymal transition (EMT). Immunohistochemical staining was performed to detect STIM1, E‑cadherin, β‑catenin and matrix metalloproteinase‑9 (MMP‑9) in 170 GC and 35 adjacent healthy gastric tissue samples. Positive staining of STIM1, E‑cadherin, β‑catenin and MMP‑9 in GC tissues was significantly greater compared with adjacent healthy tissues (P<0...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28534359/e-cadherin-in-gastric-carcinomas-relations-with-histological-parameters-and-its-prognostic-value
#12
Dimitrios Schizas, Demetrios Moris, Adamantios Michalinos, Prodromos Kanavidis, Dimitrios Oikonomou, Alexandros Papalampros, Anastasios Machairas, Theodoros Liakakos
PURPOSE: Gastric cancer (GC) is still one of the most common malignancies with the majority of the tumors being diagnosed at advanced stage. The need for identification of prognostic and early detection biomarkers is thus compulsory. E-cadherin is one of the emerging biomarkers that is currently evaluated in the literature in the frame of epithelial-mesenchymal transition (EMT). Our aim was to study the expression of E-cadherin in the various histological subtypes of GC and to evaluate its prognostic value...
March 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/28512191/mir-2392-suppresses-metastasis-and-epithelial-mesenchymal-transition-by-targeting-maml3-and-whsc1-in-gastric-cancer
#13
Jinjing Li, Tingyu Li, Yuanyuan Lu, Gaofei Shen, Hao Guo, Jian Wu, Chao Lei, Feng Du, Fenli Zhou, Xiaodi Zhao, Yongzhan Nie, Daiming Fan
MicroRNAs have emerged as essential regulators of various cellular processes. We identified the role and underlying mechanisms of miR-2392 in gastric cancer (GC) metastasis. miR-2392 was down-regulated in GC cell lines and tissues, and overexpression of miR-2392 significantly inhibited GC invasion and metastasis in vitro and in vivo We identified MAML3 and WHSC1 as novel targets of miR-2392, and knockdown of MAML3 and WHSC1 had the same antimetastatic effect as that of miR-2392 in GC cells. These effects were clinically relevant, as low miR-2392 expression was correlated with high MAML3 and WHSC1 expression and poor survival in patients with GC...
May 16, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28501849/mir-551b-regulates-epithelial-mesenchymal-transition-and-metastasis-of-gastric-cancer-by-inhibiting-erbb4-expression
#14
Guangyuan Song, Hongcheng Zhang, Chenlin Chen, Lijie Gong, Biao Chen, Shaoyun Zhao, Ji Shi, Ji Xu, Zaiyuan Ye
Epithelial-mesenchymal transition (EMT) is an important biological process that is characteristic of malignant tumor cells with metastatic potential. We investigated the role of miR-551b in EMT and metastasis in gastric cancer (GC). We found that low miR-551b levels were associated with EMT, metastasis and a poor prognosis in GC patients. Further, two GC cell lines, MNK45 and SGC7901, exhibited lower miR-551b levels than the GES normal stomach cell line. Exposing MNK45 and SGC7901 cells to TGF-β1 resulted in cell morphology changes characteristic of EMT, which was confirmed by western blot analysis demonstrating low E-Cadherin and high N-Cadherin and Vimentin levels...
April 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28501767/garlic-derived-organosulfur-compound-exerts-antitumor-efficacy-via-activation-of-mapk-pathway-and-modulation-of-cytokines-in-sgc-7901-tumor-bearing-mice
#15
Xiaoyan Jiang, Xiaosong Zhu, Weizhen Huang, Hongya Xu, Zhongxi Zhao, Siying Li, Shanzhong Li, Jianhua Cai, Jimin Cao
Diallyl trisulfide (DATS), a natural agent derived from garlic, has been tested for its antigastric cancer activities in various preliminary studies. However, more systematic pharmacodymatic (PD) and mechanistic evaluations are clearly needed. The aim of this study was to investigate the antitumor effects of DATS in the treatment of human gastric cancer cell SGC-7901 both in vitro and in vivo using widely recommended study procedures. DATS suppressed cancer cells proliferation and induced cell cycle arrest accompanied by an increase in the expressions of cyclin A2 and cyclin B1 in SGC-7901 cancer cells...
May 11, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28485805/microrna-217-suppressed-epithelial-to-mesenchymal-transition-in-gastric-cancer-metastasis-through-targeting-ptpn14
#16
Y-P Liu, X-H Sun, X-L Cao, W-W Jiang, X-X Wang, Y-F Zhang, J-L Wang
OBJECTIVE: Gastric cancer (GC) is the third leading cause of cancer-related deaths while the mechanisms underlying its metastasis are not fully understood. In this study, we aimed to explore the relationship between miR-217 and GC metastasis. PATIENTS AND METHODS: We examined miR-217 level in gastric tumor tissues of 48 patients with GC and in cell lines including gastric mucosa epithelial cell line (GES-1), gastric cancer cell line (BGC-823), and gastric cancer cell line (SGC-7901)...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28481365/membrane-bound-%C3%AE-catenin-degradation-is-enhanced-by-ets2-mediated-siah1-induction-in-helicobacter-pylori-infected-gastric-cancer-cells
#17
L Das, S B Kokate, P Dixit, S Rath, N Rout, S P Singh, S E Crowe, A Bhattacharyya
β-catenin has two different cellular functions: intercellular adhesion and transcriptional activity. The E3 ubiquitin ligase Siah1 causes ubiquitin-mediated degradation of the cytosolic β-catenin and therefore, impairs nuclear translocation and oncogenic function of β-catenin. However, the effect of Siah1 on the cell membrane bound β-catenin has not been studied. In this study, we identified that the carcinogenic bacterium H. pylori increased ETS2 transcription factor-mediated Siah1 protein expression in gastric cancer cells (GCCs) MKN45, AGS and Kato III...
May 8, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28464378/bradykinin-promotes-cell-proliferation-migration-invasion-and-tumor-growth-of-gastric-cancer-through-erk-signaling-pathway
#18
Guojun Wang, Junfeng Sun, Guanghui Liu, Yang Fu, Xiefu Zhang
Bradykinin (BK) has been reported to be involved in the progression of diverse types of cancer. In the present study, we investigated the possible role of BK in cell proliferation, migration, invasion and tumor growth of gastric cancer (GC). Cell proliferation was evaluated by MTT assays. Cell migration and invasion were assessed by Transwell assays. Tumor growth of nude mice was detected by establishing subcutaneous xenograft tumor model. Silencing of bradykinin B1 receptor (B1R) and the bradykinin B2 receptor (B2R) was performed by transfecting cells with si-B1R and si-B2R, respectively...
May 2, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28460635/transcriptomic-profiling-and-quantitative-high-throughput-qhts-drug-screening-of-cdh1-deficient-hereditary-diffuse-gastric-cancer-hdgc-cells-identify-treatment-leads-for-familial-gastric-cancer
#19
Ina Chen, Lesley Mathews-Greiner, Dandan Li, Abisola Abisoye-Ogunniyan, Satyajit Ray, Yansong Bian, Vivek Shukla, Xiaohu Zhang, Raj Guha, Craig Thomas, Berkley Gryder, Athina Zacharia, Joal D Beane, Sarangan Ravichandran, Marc Ferrer, Udo Rudloff
BACKGROUND: Patients with hereditary diffuse gastric cancer (HDGC), a cancer predisposition syndrome associated with germline mutations of the CDH1 (E-cadherin) gene, have few effective treatment options. Despite marked differences in natural history, histopathology, and genetic profile to patients afflicted by sporadic gastric cancer, patients with HDGC receive, in large, identical systemic regimens. The lack of a robust preclinical in vitro system suitable for effective drug screening has been one of the obstacles to date which has hampered therapeutic advances in this rare disease...
May 1, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28459196/relationships-of-mmp-9-e-cadherin-and-vegf-expression-with-clinicopathological-features-and-response-to-chemosensitivity-in-gastric-cancer
#20
Hongyu Gao, Xiuwen Lan, Sen Li, Yingwei Xue
The matrix metalloproteinase-9, E-cadherin, and vascular endothelial growth factor play an important role in behavior of tumor cell growth, invasion, and metastasis. In this study, we investigated the relationships of matrix metalloproteinase-9, E-cadherin, and vascular endothelial growth factor expression with clinicopathological features and results of chemosensitivity tested by collagen gel droplet-embedded culture-drug sensitivity test in gastric cancer. Fresh specimens were used for collagen gel droplet-embedded culture-drug sensitivity test and paired fixed specimens were used for immunohistochemistry...
May 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
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