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https://www.readbyqxmd.com/read/29150449/claudin-10-isoform-expression-and-cation-selectivity-change-with-salinity-in-salt-secreting-epithelia-of-f-heteroclitus
#1
William S Marshall, Jason P Breves, Ellen M Doohan, Christian K Tipsmark, Scott P Kelly, George N Robertson, Patricia M Schulte
To provide insight into claudin (Cldn) tight junction (TJ) protein contributions to branchial salt secretion in marine teleost fishes, this study examined cldn-10 TJ protein isoforms of a euryhaline teleost (mummichog; Fundulus heteroclitus) in association with salinity change and measurements of transepithelial cation selectivity. Mummichogs were transferred from fresh water (FW) to seawater (SW, 35 ‰) and from SW to hypersaline SW (2SW, 60 ‰) in a time course with transfer control groups (FW to FW and SW to SW)...
November 17, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29106043/synthesis-and-application-of-a-novel-solid-phase-extraction-adsorbent-for-the-multiresidue-analysis-of-insecticides-in-water
#2
Jingjing Xiong, Huizhen Li, Xue Ma, Jing You
A new solid-phase adsorbent was synthesized for the simultaneous enrichment of multiple classes of trace insecticides (neonicotinoids, organophosphates, fiproles and organochlorines) in water. The adsorbent was spherical with a diameter, surface area, average pore volume and pore size of ∼5 μm, 341 m(2) /g, 0.092 m(3) /g and 2.22 nm, respectively. Extraction conditions were optimized, including water pH and the type and volume of the rinsing and eluting solvents. After extraction, target insecticides were analyzed by gas chromatography with mass spectrometry and high-performance liquid chromatography with tandem mass spectrometry...
November 6, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29105862/co-cluster-based-metal-organic-frameworks-as-selective-catalysts-for-benzene-tandem-acylation-nazarov-cyclization-to-benzocyclopentanone
#3
Jie Wu, Mengjia Liu, Kuan Gao, Yanru Fan, Xiaoqing Guo, Xiangru Meng, Hongwei Hou
One-step selective benzene acylation-Nazarov cyclization is an attractive yet challenging method for the synthesis of benzocyclopentanone skeleton which is key intermediate of many natural products. Herein, we reported that two metal-cluster-based metal-organic frameworks (MCOFs) {(H3O)2[Co4(pbcd)2(μ3-OH)2]·(CH3CH2OH)·4H2O}n (1) and {[Co5.25(mcd)2(HCO2)(μ2-O)0.5(μ3-OH)0.5(H2O)4]·6H2O·5DMF}n (2) were developed to catalyze tandem-Nazarov cyclization reaction of 1,3-dimethoxy benzene with α,β-unsaturated carboxylic acids for the synthesis of cyclopentenone[b]benzene...
November 5, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29016681/heterodimerization-of-two-pore-domain-k-channel-task1-and-talk2-in-living-heterologous-expression-systems
#4
Yoshiaki Suzuki, Kanako Tsutsumi, Tatsuya Miyamoto, Hisao Yamamura, Yuji Imaizumi
Two-pore-domain K+ (K2P) channels sense a wide variety of stimuli such as mechanical stress, inhalational anesthetics, and changes in extracellular pH or temperature. The K2P channel activity forms a background K+ current and, thereby, contributes to resting membrane potentials. Six subfamilies including fifteen subtypes of K2P channels have been identified. Each K2P channel molecule with two pores consists of a homodimer of each subtype. In addition, a few heterodimers mainly within the same subfamilies have been found recently...
2017: PloS One
https://www.readbyqxmd.com/read/28970050/the-biology-of-mucus-composition-synthesis-and-organization
#5
Rama Bansil, Bradley S Turner
In this review we discuss mucus, the viscoelastic secretion from goblet or mucous producing cells that lines the epithelial surfaces of all organs exposed to the external world. Mucus is a complex aqueous fluid that owes its viscoelastic, lubricating and hydration properties to the glycoprotein mucin combined with electrolytes, lipids and other smaller proteins. Electron microscopy of mucosal surfaces reveals a highly convoluted surface with a network of fibers and pores of varying sizes. The major structural and functional component, mucin is a complex glycoprotein coded by about 20 mucin genes which produce a protein backbone having multiple tandem repeats of Serine, Threonine (ST repeats) where oligosaccharides are covalently O-linked...
September 29, 2017: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/28863334/molecularly-imprinted-polymer-sol-gel-tablet-toward-micro-solid-phase-extraction-ii-determination-of-amphetamine-in-human-urine-samples-by-liquid-chromatography-tandem-mass-spectrometry
#6
Aziza El-Beqqali, Lars I Andersson, Amin Dadoun Jeppsson, Mohamed Abdel-Rehim
Amphetamine selective molecularly imprinted sol-gel polymer tablets, MIP-tablets, for solid-phase microextraction of biofluid samples were prepared. An acetonitrile solution of deuterated amphetamine template and silane precursor, 3-(propylmethacrylate) trimethoxysilane, was soaked into the pores of polyethylene tablet substrates and polymerized by an acid-catalysed sol-gel process. Application of the resultant MIP-tablets to extract amphetamine from human urine samples followed by LC-MS/MS analysis was investigated...
September 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28855917/peptidomic-analysis-of-the-venom-of-the-solitary-bee-xylocopa-appendiculata-circumvolans
#7
Kohei Kazuma, Kenji Ando, Ken-Ichi Nihei, Xiaoyu Wang, Marisa Rangel, Marcia Regina Franzolin, Kanami Mori-Yasumoto, Setsuko Sekita, Makoto Kadowaki, Motoyoshi Satake, Katsuhiro Konno
BACKGROUND: Among the hymenopteran insect venoms, those from social wasps and bees - such as honeybee, hornets and paper wasps - have been well documented. Their venoms are composed of a number of peptides and proteins and used for defending their nests and themselves from predators. In contrast, the venoms of solitary wasps and bees have not been the object of further research. In case of solitary bees, only major peptide components in a few venoms have been addressed. Therefore, the aim of the present study was to explore the peptide component profile of the venom from the solitary bee Xylocopa appendiculata circumvolans by peptidomic analysis with using LC-MS...
2017: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/28798962/structural-pathway-of-regulated-substrate-transfer-and-threading-through-an-hsp100-disaggregase
#8
Célia Deville, Marta Carroni, Kamila B Franke, Maya Topf, Bernd Bukau, Axel Mogk, Helen R Saibil
Refolding aggregated proteins is essential in combating cellular proteotoxic stress. Together with Hsp70, Hsp100 chaperones, including Escherichia coli ClpB, form a powerful disaggregation machine that threads aggregated polypeptides through the central pore of tandem adenosine triphosphatase (ATPase) rings. To visualize protein disaggregation, we determined cryo-electron microscopy structures of inactive and substrate-bound ClpB in the presence of adenosine 5'-O-(3-thiotriphosphate), revealing closed AAA+ rings with a pronounced seam...
August 2017: Science Advances
https://www.readbyqxmd.com/read/28714232/intermediate-product-regulation-in-tandem-solid-catalysts-with-multimodal-porosity-for-high-yield-synthetic-fuel-production
#9
Nicolas Duyckaerts, Mathias Bartsch, Ioan-Teodor Trotuş, Norbert Pfänder, Axel Lorke, Ferdi Schüth, Gonzalo Prieto
Tandem catalysis is an attractive strategy to intensify chemical technologies. However, simultaneous control over the individual and concerted catalyst performances poses a challenge. We demonstrate that enhanced pore transport within a Co/Al2 O3 Fischer-Tropsch (FT) catalyst with hierarchical porosity enables its tandem integration with a Pt/ZSM-5 zeolitic hydrotreating catalyst in a spatially distant fashion that allows for catalyst-specific temperature adjustment. Nevertheless, this system resembles the case of close active-site proximity by mitigating secondary reactions of primary FT α-olefin products...
September 11, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28699173/porous-silicon-nanoparticle-delivery-of-tandem-peptide-anti-infectives-for-the-treatment-of-pseudomonas-aeruginosa-lung-infections
#10
Ester J Kwon, Matthew Skalak, Alessandro Bertucci, Gary Braun, Francesco Ricci, Erkki Ruoslahti, Michael J Sailor, Sangeeta N Bhatia
There is an urgent need for new materials to treat bacterial infections. In order to improve antibacterial delivery, an anti-infective nanomaterial is developed that utilizes two strategies for localization: i) a biodegradable nanoparticle carrier to localize therapeutics within the tissue, and ii) a novel tandem peptide cargo to localize payload to bacterial membranes. First, a library of antibacterial peptides is screened that combines a membrane-localizing peptide with a toxic peptide cargo and discovers a tandem peptide that displays synergy between the two domains and is able to kill Pseudomonas aeruginosa at sub-micromolar concentrations...
September 2017: Advanced Materials
https://www.readbyqxmd.com/read/28680078/3d-printed-silicones-with-shape-memory
#11
Amanda S Wu, Ward Small Iv, Taylor M Bryson, Emily Cheng, Thomas R Metz, Stephanie E Schulze, Eric B Duoss, Thomas S Wilson
Direct ink writing enables the layer-by-layer manufacture of ordered, porous structures whose mechanical behavior is driven by architecture and material properties. Here, we incorporate two different gas filled microsphere pore formers to evaluate the effect of shell stiffness and Tg on compressive behavior and compression set in siloxane matrix printed structures. The lower Tg microsphere structures exhibit substantial compression set when heated near and above Tg, with full structural recovery upon reheating without constraint...
July 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28676376/cutaneous-inflammation-regulates-thik1-expression-in-small-c-like-nociceptor-dorsal-root-ganglion-neurons
#12
William Haskins, Sergio Benitez, Juan M Mercado, Cristian G Acosta
Tandem pore-domain Halothane Inhibited K(+) channel (THIK1) is a two-pore-domain potassium channel (K2P) present in dorsal root ganglia (DRG). We previously demonstrated that THIK1 mRNA levels in the DRG dropped ipsilaterally 1day after CFA-induced cutaneous inflammation (CFA1). In this study we aimed to identify the currently unknown DRG subpopulations expressing THIK1, and to investigate the relationship between the channel and both inflammatory and spontaneous pain in normal rats. Using a combination of immunohistochemistry, western blotting and behavioural tests, we found that all small neurons and large groups of medium and large DRG neurons express THIK1...
July 1, 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/28630209/functional-task-3-like-channels-in-mitochondria-of-aldosterone-producing-zona-glomerulosa-cells
#13
Junlan Yao, David McHedlishvili, William E McIntire, Nick A Guagliardo, Alev Erisir, Craig A Coburn, Vincent P Santarelli, Douglas A Bayliss, Paula Q Barrett
Ca(2+) drives aldosterone synthesis in the cytosolic and mitochondrial compartments of the adrenal zona glomerulosa cell. Membrane potential across each of these compartments regulates the amplitude of the Ca(2+) signal; yet, only plasma membrane ion channels and their role in regulating cell membrane potential have garnered investigative attention as pathological causes of human hyperaldosteronism. Previously, we reported that genetic deletion of TASK-3 channels (tandem pore domain acid-sensitive K(+) channels) from mice produces aldosterone excess in the absence of a change in the cell membrane potential of zona glomerulosa cells...
August 2017: Hypertension
https://www.readbyqxmd.com/read/28591601/origin-of-ion-specificity-of-telomeric-dna-g-quadruplexes-investigated-by-free-energy-simulations
#14
Till Siebenmorgen, Martin Zacharias
Telomeric DNA consists of tandem repeats of the sequence d(TTAGGG) that form G-quadruplex structures made of stacked guanines with monovalent cations bound at a central cavity. Although different ions can stabilize a G-quadruplex structure, the preferred bound ions are typically K(+) or Na(+). Several different strand-folding topologies have been reported for Q-quadruplexes formed from telomeric repeats depending on DNA length and ion solution condition. This suggests a possible dependence of the ion selectivity of the central pore on the folding topology of the quadruplex...
June 6, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28381826/recombinant-tandem-of-pore-domains-in-a-weakly-inward-rectifying-k-channel-2-twik2-forms-active-lysosomal-channels
#15
Nicole Bobak, Sylvain Feliciangeli, Cheng-Chang Chen, Ismail Ben Soussia, Stefan Bittner, Sophie Pagnotta, Tobias Ruck, Martin Biel, Christian Wahl-Schott, Christian Grimm, Sven G Meuth, Florian Lesage
Recombinant TWIK2 channels produce weak basal background K(+) currents. Current amplitudes depend on the animal species the channels have been isolated from and on the heterologous system used for their re-expression. Here we show that this variability is due to a unique cellular trafficking. We identified three different sequence signals responsible for the preferential expression of TWIK2 in the Lamp1-positive lysosomal compartment. Sequential inactivation of tyrosine-based (Y308ASIP) and di-leucine-like (E266LILL and D282EDDQVDIL) trafficking motifs progressively abolishes the targeting of TWIK2 to lysosomes, and promotes its functional relocation at the plasma membrane...
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28346390/the-effect-and-mechanism-of-transdermal-penetration-enhancement-of-fu-s-cupping-therapy-new-physical-penetration-technology-for-transdermal-administration-with-traditional-chinese-medicine-tcm-characteristics
#16
Wei-Jie Xie, Yong-Ping Zhang, Jian Xu, Xiao-Bo Sun, Fang-Fang Yang
BACKGROUND: In this paper, a new type of physical penetration technology for transdermal administration with traditional Chinese medicine (TCM) characteristics is presented. Fu's cupping therapy (FCT), was established and studied using in vitro and in vivo experiments and the penetration effect and mechanism of FCT physical penetration technology was preliminarily discussed. METHODS: With 1-(4-chlorobenzoyl)-5-methoxy-2-methylindole-3-ylacetic acid (indomethacin, IM) as a model drug, the establishment of high, medium, and low references was completed for the chemical permeation system via in vitro transdermal tests...
March 27, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28325748/halogenated-ether-alcohol-and-alkane-anesthetics-activate-task-3-tandem-pore-potassium-channels-likely-through-a-common-mechanism
#17
Anita Luethy, James D Boghosian, Rithu Srikantha, Joseph F Cotten
The TWIK-related acid-sensitive potassium channel 3 (TASK-3; KCNK9) tandem pore potassium channel function is activated by halogenated anesthetics through binding at a putative anesthetic-binding cavity. To understand the pharmacologic requirements for TASK-3 activation, we studied the concentration-response of TASK-3 to several anesthetics (isoflurane, desflurane, sevoflurane, halothane, α-chloralose, 2,2,2-trichloroethanol [TCE], and chloral hydrate), to ethanol, and to a panel of halogenated methanes and alcohols...
June 2017: Molecular Pharmacology
https://www.readbyqxmd.com/read/28258564/the-evolutionary-aspects-of-aquaporin-family
#18
REVIEW
Kenichi Ishibashi, Yoshiyuki Morishita, Yasuko Tanaka
Aquaporins (AQPs ) are a family of transmembrane proteins present in almost all species including virus. They are grossly divided into three subfamilies based on the sequence around a highly conserved pore-forming NPA motif: (1) classical water -selective AQP (CAQP), (2) glycerol -permeable aquaglyceroporin (AQGP) and (3) AQP super-gene channel, superaquaporin (SAQP). AQP is composed of two tandem repeats of conserved three transmembrane domains and a NPA motif. AQP ancestors probably started in prokaryotes by the duplication of half AQP genes to be diversified into CAQPs or AQGPs by evolving a subfamily-specific carboxyl-terminal NPA motif...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28193058/development-of-a-biocompatible-in-tube-solid-phase-microextraction-device-a-sensitive-approach-for-direct-analysis-of-single-drops-of-complex-matrixes
#19
Hamed Piri-Moghadam, Sofia Lendor, Janusz Pawliszyn
The aim of the current study is to develop a sensitive solid-phase microextraction (SPME) device for direct and rapid analysis of untreated complex matrixes (i.e., single drop of the samples, V ≤ 2 μL). A thin layer of a biocompatible nanostructured polypyrrole (PPy) was electrochemically deposited inside a medical grade spinal needle, minimizing the matrix effect. Microsampling was facilitated by loading the sample inside the in-tube SPME device (withdraw of sample via plunger), where extraction was performed under static conditions...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28185621/an-alternative-perspective-of-hollow-fiber-mediated-extraction-bundled-hollow-fiber-array-liquid-phase-microextraction-with-sonication-assisted-desorption-and-liquid-chromatography-tandem-mass-spectrometry-for-determination-of-estrogens-in-aqueous-matrices
#20
Shalene Xue Lin Goh, Hian Kee Lee
A bundled hollow fiber array (BHF)-liquid-phase microextraction (LPME) approach has been developed for the ultra-high performance liquid chromatography tandem mass spectrometric determination of estrone, 17β-estradiol, estriol, and 17α-ethinylestradiol. The BHF was dipped in n-octanol to impregnate only the wall pores of the hollow fibers without deliberate loading of extractant solvent in the lumens, before placing it in the sample for extraction. Parameters influencing extraction efficiency, such as number of bundled hollow fibers, type of extraction and desorption solvent, agitation mode, extraction temperature and duration, and the salting out effect were examined...
March 10, 2017: Journal of Chromatography. A
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