keyword
MENU ▼
Read by QxMD icon Read
search

Endocytic trafficking

keyword
https://www.readbyqxmd.com/read/29608571/endocytic-recycling-via-the-tgn-underlies-the-polarized-hyphal-mode-of-life
#1
Miguel Hernández-González, Ignacio Bravo-Plaza, Mario Pinar, Vivian de Los Ríos, Herbert N Arst, Miguel A Peñalva
Intracellular traffic in Aspergillus nidulans hyphae must cope with the challenges that the high rates of apical extension (1μm/min) and the long intracellular distances (>100 μm) impose. Understanding the ways in which the hyphal tip cell coordinates traffic to meet these challenges is of basic importance, but is also of considerable applied interest, as fungal invasiveness of animals and plants depends critically upon maintaining these high rates of growth. Rapid apical extension requires localization of cell-wall-modifying enzymes to hyphal tips...
April 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29608564/actin-organization-and-endocytic-trafficking-are-controlled-by-a-network-linking-nima-related-kinases-to-the-cdc-42-sid-3-ack1-pathway
#2
Vladimir Lažetić, Braveen B Joseph, Sarina M Bernazzani, David S Fay
Molting is an essential process in the nematode Caenorhabditis elegans during which the epidermal apical extracellular matrix, termed the cuticle, is detached and replaced at each larval stage. The conserved NIMA-related kinases NEKL-2/NEK8/NEK9 and NEKL-3/NEK6/NEK7, together with their ankyrin repeat partners, MLT-2/ANKS6, MLT-3/ANKS3, and MLT-4/INVS, are essential for normal molting. In nekl and mlt mutants, the old larval cuticle fails to be completely shed, leading to entrapment and growth arrest. To better understand the molecular and cellular functions of NEKLs during molting, we isolated genetic suppressors of nekl molting-defective mutants...
April 2, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29599122/intersectin-goes-nuclear-secret-life-of-an-endocytic-protein
#3
Gualtiero Alvisi, Lucia Paolini, Andrea Contarini, Chiara Zambarda, Veronica Di Antonio, Antonella Colosini, Nicole Mercandelli, Martina Timmoneri, Giorgio Palù, Luigi Caimi, Doris Ricotta, Annalisa Radeghieri
Intersectin 1-short (ITSN1-s) is a 1220 amino acids ubiquitously-expressed scaffold protein presenting a multi-domain structure that allows to spatiotemporally regulate the functional interaction of a plethora of proteins. Beside its well-established role in endocytosis, ITSN1-s is involved in regulation of cell signaling and is implicated in tumorigenesis processes, although the signaling pathways involved are still poorly understood. Here we identify ITSN1-s as a nucleocytoplasmic trafficking protein. We show that, by binding to importin (IMP)a, a small fraction of ITSN1-s localizes in the cell nucleus at the steady state, where it preferentially associates with the nuclear envelope (NE) and interacts with lamin A/C...
March 29, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29590595/entropic-control-of-receptor-recycling-using-engineered-ligands
#4
Andre C M DeGroot, David J Busch, Carl C Hayden, Samuel A Mihelic, Aaron T Alpar, Marcelo Behar, Jeanne C Stachowiak
Receptor internalization by endocytosis regulates diverse cellular processes, from the rate of nutrient uptake to the timescale of essential signaling events. The established view is that internalization is tightly controlled by specific protein-binding interactions. However, recent work suggests that physical aspects of receptors influence the process in ways that cannot be explained by biochemistry alone. Specifically, work from several groups suggests that increasing the steric bulk of receptors may inhibit their uptake by multiple types of trafficking vesicles...
March 27, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29587629/comparative-analysis-of-fkbp-family-protein-evaluation-structure-and-function-in-mammals-and-drosophila-melanogaster
#5
REVIEW
George Ghartey-Kwansah, Zhongguang Li, Rui Feng, Liyang Wang, Xin Zhou, Frederic Z Chen, Meng Meng Xu, Odell Jones, Yulian Mu, Shawn Chen, Joseph Bryant, Williams B Isaacs, Jianjie Ma, Xuehong Xu
BACKGROUND: FK506-binding proteins (FKBPs) have become the subject of considerable interest in several fields, leading to the identification of several cellular and molecular pathways in which FKBPs impact prenatal development and pathogenesis of many human diseases. MAIN BODY: This analysis revealed differences between how mammalian and Drosophila FKBPs mechanisms function in relation to the immunosuppressant drugs, FK506 and rapamycin. Differences that could be used to design insect-specific pesticides...
March 27, 2018: BMC Developmental Biology
https://www.readbyqxmd.com/read/29582527/rab11-mediates-selective-recycling-and-endocytic-trafficking-in-trypanosoma-brucei
#6
Khan Umaer, Peter J Bush, James D Bangs
Trypanosoma brucei possesses a streamlined secretory system that guarantees efficient delivery to the cell surface of the critical GPI-anchored virulence factors, variable surface glycoprotein (VSG) and transferrin receptor (TfR). Both are thought to be constitutively endocytosed and returned to the flagellar pocket via TbRab11+ recycling endosomes. We use conditional knockdown with established reporters to investigate the role of TbRab11 in specific endomembrane trafficking pathways in bloodstream trypanosomes...
March 26, 2018: Traffic
https://www.readbyqxmd.com/read/29572244/endosome-maturation-factors-rabenosyn-5-vps45-and-caveolin-1-regulate-ciliary-membrane-and-polycystin-2-homeostasis
#7
Noémie Scheidel, Julie Kennedy, Oliver E Blacque
Primary cilium structure and function relies on control of ciliary membrane homeostasis, regulated by membrane trafficking processes that deliver and retrieve ciliary components at the periciliary membrane. However, the molecular mechanisms controlling ciliary membrane establishment and maintenance, especially in relation to endocytosis, remain poorly understood. Here, using Caenorhabditis elegans , we describe closely linked functions for early endosome (EE) maturation factors RABS-5 (Rabenosyn-5) and VPS-45 (VPS45) in regulating cilium length and morphology, ciliary and periciliary membrane volume, and ciliary signalling-related sensory behaviour...
March 23, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29569114/the-phox-homology-px-domain
#8
Mintu Chandra, Brett M Collins
The phox-homology (PX) domain is a phosphoinositide-binding domain conserved in all eukaryotes and present in 49 human proteins. Proteins containing PX domains, many of which are also known as sorting nexins (SNXs), have a large variety of functions in membrane trafficking, cell signaling, and lipid metabolism in association with membranes of the secretory and endocytic system. In this review we discuss the structural basis for both canonical lipid interactions with the endosome-enriched lipid phosphatidylinositol-3-phosphate (PtdIns3P) as well as non-canonical lipids that promote membrane association...
March 23, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29564371/tracking-endocytosis-and-intracellular-trafficking-of-epitope-tagged-syntaxin-3-by-antibody-feeding-in-live-polarized-mdck-cells
#9
Adrian J Giovannone, Elena Reales, Pallavi Bhattaram, Alberto Fraile-Ramos, Thomas Weimbs
The uptake and trafficking of cell surface receptors can be monitored by a technique called 'antibody-feeding' which uses an externally applied antibody to label the receptor on the surface of cultured, live cells. Here, we adapt the traditional antibody-feeding experiment to polarized epithelial cells (Madin-Darby Canine Kidney) grown on permeable Transwell supports. By adding two tandem extracellular Myc epitope tags to the C-terminus of the SNARE protein syntaxin 3 (Stx3), we provided a site where an antibody could bind, allowing us to perform antibody-feeding experiments on cells with distinct apical and basolateral membranes...
February 5, 2018: Bio-protocol
https://www.readbyqxmd.com/read/29562775/restoring-in-vivo-like-membrane-lipidomics-promotes-exosome-trophic-behavior-from-human-placental-mesenchymal-stromal-stem-cells
#10
Claudia Cavallini, Chiara Zannini, Elena Olivi, Riccardo Tassinari, Valentina Taglioli, Martina Rossi, Paola Poggi, Alexandros Chatgilialoglu, Giuliana Simonazzi, Francesco Alviano, Laura Bonsi, Carlo Ventura
Human mesenchymal stem cells (hMSCs) are an effective tool in regenerative medicine notably for their intrinsic plentiful paracrine activity rather than differentiating properties. The hMSC secretome includes a wide spectrum of regulatory and trophic factors, encompassing several naked molecules as well as different kinds of extracellular vesicles (EVs). Among EVs, exosomes represent an intriguing population, able to shuttle proteins, transcription factors, and genetic materials, with a relevant role in cell-to-cell communication, modulating biological responses in recipient cells...
January 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29559974/viral-mirnas-alter-host-cell-mirna-profiles-and-modulate-innate-immune-responses
#11
Afsar R Naqvi, Jennifer Shango, Alexandra Seal, Deepak Shukla, Salvador Nares
Prevalence of the members of herpesvirus family in oral inflammatory diseases is increasingly acknowledged suggesting their likely role as an etiological factor. However, the underlying mechanisms remain obscure. In our recent miRNA profiling of healthy and diseased human tooth pulps, elevated expression of human herpesvirus encoded viral microRNAs (v-miRs) were identified. Based on the fold induction and significance values, we selected three v-miRs namely miR-K12-3-3p [Kaposi sarcoma-associated virus (KSHV)], miR-H1 [herpes simplex virus 1 (HSV1)], and miR-UL-70-3p [human cytomegalovirus (HCMV)] to further examine their impact on host cellular functions...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29551457/highly-specific-detection-of-muscarinic-m3-receptor-g-protein-interaction-and-intracellular-trafficking-in-human-detrusor-using-proximity-ligation-assay-pla
#12
Mandy Berndt-Paetz, Luise Herbst, Annett Weimann, Andreas Gonsior, Jens-Uwe Stolzenburg, Jochen Neuhaus
PURPOSE: Muscarinic acetylcholine receptors (mAChRs) regulate a number of important physiological functions. Alteration of mAChR expression or function has been associated in the etiology of several pathologies including functional bladder disorders (e.g bladder pain syndrome/interstitial cystitis - BPS/IC). In a previous study we found specific mAChR expression patterns associated with BPS/IC, while correlation between protein and gene expression was lacking. Posttranslational regulatory mechanisms, e...
March 15, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29549047/the-breast-cancer-antigen-5t4-interacts-with-rab11-and-is-a-target-and-regulator-of-rab11-mediated-trafficking
#13
Janelle L Harris, Keyur Dave, Jeffrey Gorman, Kum Kum Khanna
BACKGROUND AND AIMS: 5T4 is a transmembrane glycoprotein with limited expression in normal adult tissues and expression in some solid tumours. It is unclear whether 5T4 is preferentially expressed by stem or differentiated cell types. Modes of 5T4 regulation are unknown despite its ongoing development as a cancer immunotherapy target. Our aims were to clarify the differentiation status of 5T4 expressing cells in breast cancer and to understand the mechanism underlying 5T4 membrane presentation...
March 13, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29548969/tracheal-expression-of-osiris-gene-family-in-drosophila
#14
Aaron Scholl, Yuyang Yang, Patrick McBride, Kelly Irwin, Lan Jiang
The Drosophila trachea is a premier genetic system to investigate the fundamental mechanisms of tubular organ formation. Development of the trachea consists of the well understood early branch specification and migration processes, and the less clear later branch maturation process including the apical membrane expansion, cytoskeleton rearrangement, luminal matrix clearance, and air-filling. We identified seven members of the Osiris (Osi) gene family with obvious tracheal expression in trachea. In addition, HA-tagged Osi proteins are highly concentrated in vesicle-like structures at and near the apical membrane...
March 13, 2018: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/29545069/ascorbic-acid-increases-svct2-localization-at-the-plasma-membrane-by-accelerating-its-trafficking-from-early-secretory-compartments-and-through-the-endocytic-recycling-pathway
#15
A Covarrubias-Pinto, A I Acuña, G Boncompain, E Pápic, P V Burgos, F Perez, M A Castro
Ascorbic acid (Asc) is an antioxidant molecule essential for physiological functions. The concentration of extracellular Asc increases during synaptic transmission and renal reabsorption. These phenomena induce an increase of the Sodium-dependent-Vitamin-C-transporter 2 (SVCT2) at plasma membrane (PM) localization, as we previously demonstrated in neuronal and non-neuronal cells. Hence, the aim of this study was to evaluate intracellular SVCT2 trafficking kinetics in response to Asc. We observed two peaks of SVCT2 localization and function at the PM (at 5-10min, "acute response", and 30-60min, "post-acute response") when cells were incubated with Asc...
March 12, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29535204/interactions-between-the-hepatitis-c-virus-nonstructural-2-protein-and-host-adaptor-proteins-1-and-4-orchestrate-virus-release
#16
Fei Xiao, Stanley Wang, Rina Barouch-Bentov, Gregory Neveu, Szuyuan Pu, Melanie Beer, Stanford Schor, Sathish Kumar, Vlad Nicolaescu, Brett D Lindenbach, Glenn Randall, Shirit Einav
Hepatitis C virus (HCV) spreads via secreted cell-free particles or direct cell-to-cell transmission. Yet, virus-host determinants governing differential intracellular trafficking of cell-free- and cell-to-cell-transmitted virus remain unknown. The host adaptor proteins (APs) AP-1A, AP-1B, and AP-4 traffic in post-Golgi compartments, and the latter two are implicated in basolateral sorting. We reported that AP-1A mediates HCV trafficking during release, whereas the endocytic adaptor AP-2 mediates entry and assembly...
March 13, 2018: MBio
https://www.readbyqxmd.com/read/29523688/proteomics-reveals-novel-protein-associations-with-early-endosomes-in-an-epidermal-growth-factor-dependent-manner
#17
Julie A Gosney, Daniel W Wilkey, Michael L Merchant, Brian P Ceresa
The epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that is an integral component of proliferative signaling. EGFRs on the cell surface become activated upon epidermal growth factor (EGF) binding and have an increased rate of endocytosis. Once in the cytoplasm, the EGF:EGFR complex is trafficked to the lysosome for degradation and signaling is terminated. During trafficking, the EGFR kinase domain remains active and the internalized EGFR can continue signaling to downstream effectors. While effector activity varies based on the EGFR's endocytic location, it is not clear how this occurs...
March 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29515354/mechanisms-of-%C3%AE-synuclein-induced-synaptopathy-in-parkinson-s-disease
#18
REVIEW
Jessika C Bridi, Frank Hirth
Parkinson's disease (PD) is characterized by intracellular inclusions of aggregated and misfolded α-Synuclein (α-Syn), and the loss of dopaminergic (DA) neurons in the brain. The resulting motor abnormalities mark the progression of PD, while non-motor symptoms can already be identified during early, prodromal stages of disease. Recent studies provide evidence that during this early prodromal phase, synaptic and axonal abnormalities occur before the degenerative loss of neuronal cell bodies. These early phenotypes can be attributed to synaptic accumulation of toxic α-Syn...
2018: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29515184/alteration-of-endosomal-trafficking-is-associated-with-early-onset-parkinsonism-caused-by-synj1-mutations
#19
Dominga Fasano, Silvia Parisi, Giovanna Maria Pierantoni, Anna De Rosa, Marina Picillo, Giuseppina Amodio, Maria Teresa Pellecchia, Paolo Barone, Ornella Moltedo, Vincenzo Bonifati, Giuseppe De Michele, Lucio Nitsch, Paolo Remondelli, Chiara Criscuolo, Simona Paladino
Recently, a new form of autosomal recessive early-onset parkinsonism (PARK20), due to mutations in the gene encoding the phosphoinositide phosphatase, Synaptojanin 1 (Synj1), has been reported. Several genes responsible for hereditary forms of Parkinson's disease are implicated in distinct steps of the endolysosomal pathway. However, the nature and the degree of endocytic membrane trafficking impairment in early-onset parkinsonism remains elusive. Here, we show that depletion of Synj1 causes drastic alterations of early endosomes, which become enlarged and more numerous, while it does not affect the morphology of late endosomes both in non-neuronal and neuronal cells...
March 7, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29514933/the-yeast-arrestin-related-protein-bul1-is-a-novel-actor-of-glucose-induced-endocytosis
#20
Junie Hovsepian, Véronique Albanèse, Michel Becuwe, Vasyl Ivashov, David Teis, Sébastien Léon
Yeast cells have a remarkable ability to adapt to nutritional changes in their environment. During adaptation, nutrient-signalling pathways drive the selective endocytosis of nutrient transporters present at the cell surface. A current challenge is to understand the mechanistic basis of this regulation. Transporter endocytosis is triggered by their ubiquitylation, which involves the ubiquitin ligase Rsp5 and its adaptors of the arrestin-related family (ART). This step is highly regulated by nutrient availability...
March 7, 2018: Molecular Biology of the Cell
keyword
keyword
96371
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"