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Endocytic trafficking

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https://www.readbyqxmd.com/read/28724764/rab5-and-rab11-are-required-for-clathrin-dependent-endocytosis-of-japanese-encephalitis-virus-in-bhk-21-cells
#1
Chun-Chun Liu, Yun-Na Zhang, Zhao-Yao Li, Jin-Xiu Hou, Jing Zhou, Lin Kan, Bin Zhou, Pu-Yan Chen
During infection Japanese Encephalitis Virus generally enters host cells via receptor-mediated clathrin dependent endocytosis. The trafficking of JEV within endosomes is controlled by Rab GTPases, but which Rab proteins are involved in JEV entry into BHK-21 cells is unknown. In this study, entry and post-internalization of JEV were analyzed using biochemical inhibitors, RNA interference and dominant negative mutants. Our data demonstrate that JEV entry into BHK-21 cells depends on clathrin, dynamin, and cholesterol, but not caveolae or macropinocytosis...
July 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28724758/the-shcd-phosphotyrosine-adaptor-subverts-canonical-egf-receptor-trafficking
#2
Melanie K B Wills, Hayley R Lau, Nina Jones
Shc family signalling adaptors connect activated transmembrane receptors to proximal effectors, and most also contain a sequence involved in clathrin-mediated receptor endocytosis. Notably, this AP2 adaptin binding motif (AD) is absent from the ShcD homolog, which also uniquely promotes ligand-independent phosphorylation of the Epidermal Growth Factor (EGF) Receptor (EGFR). We now report that cultured cells expressing ShcD exhibit reduced EGF uptake, commensurate with a decrease in EGFR surface presentation...
July 19, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28720662/proximal-tubule-apical-endocytosis-is-modulated-by-fluid-shear-stress-via-an-mtor-dependent-pathway
#3
Kimberly R Long, Katherine E Shipman, Youssef Rbaibi, Elizabeth V Menshikova, Vladimir B Ritov, Megan L Eshbach, Yu Jiang, Edwin K Jackson, Catherine J Baty, Ora A Weisz
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular filtration rate (GFR) to maintain glomerulotubular balance. PT cells in culture upregulate endocytic capacity in response to acute changes in fluid shear stress (FSS); however it is not known whether GFR modulates PT endocytosis to enable maximally efficient uptake of filtered proteins in vivo Here we show that cells cultured under continuous FSS develop an expanded apical endocytic pathway as well as increased endocytic capacity and lysosomal biogenesis...
July 18, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28713398/a-hops-protein-movps41-is-crucially-important-for-vacuolar-morphogenesis-vegetative-growth-reproduction-and-virulence-in-magnaporthe-oryzae
#4
Xiaojie Zhang, Guanghui Wang, Chengdong Yang, Jun Huang, Xiaofeng Chen, Jie Zhou, Guangpu Li, Justice Norvienyeku, Zonghua Wang
The homotypic fusion and protein sorting protein complex (HOPS) is the first known tether complex identified in the endocytic system that plays a key role in promoting homotypic vacuolar fusion, vacuolar biogenesis and trafficking in a wide range of organisms, including plant and fungi. However, the exact influence of the HOPS complex on growth, reproduction and pathogenicity of the economically destructive rice blast fungus has not been investigated. In this study, we identified M. oryzae vacuolar protein sorting 41 (MoVps41) an accessory subunit of HOPS complex and used targeted gene deletion approach to evaluate its contribution to growth, reproduction and infectious life cycle of the rice blast fungus...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28705836/essential-and-selective-role-of-snx12-in-transport-of-endocytic-and-retrograde-cargo
#5
Amulya Priya, Jini Sugatha, Sameena Parveen, Sandra Lacas-Gervais, Prateek Raj, Jérôme Gilleron, Sunando Datta
The endosomal protein sorting machineries play vital roles in diverse physiologically important cellular processes. Much of the core membrane sorting apparatus are conserved in evolution, such as retromer, involved in the recycling of a diverse set of cargoes via retrograde trafficking route. Here, using a RNAi based loss of function study, we identified that SNX12 when suppressed, leads to severe blockage in CIM6PR transport and alters the morphology of the endocytic compartments. We demonstrate that SNX12 is involved in the early phase of CIM6PR transport and mediates receptor recycling upstream of the other well established SNX components of retromer...
July 13, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28704415/c-elegans-sma-10-regulates-bmp-receptor-trafficking
#6
Ryan J Gleason, Mehul Vora, Ying Li, Nanci S Kane, Kelvin Liao, Richard W Padgett
Signal transduction of the conserved transforming growth factor-β (TGFβ) family signaling pathway functions through two distinct serine/threonine transmembrane receptors, the type I and type II receptors. Endocytosis orchestrates the assembly of signaling complexes by coordinating the entry of receptors with their downstream signaling mediators. Recently, we showed that the C. elegans type I bone morphogenetic protein (BMP) receptor SMA-6, part of the TGFβ family, is recycled through the retromer complex while the type II receptor, DAF-4 is recycled in a retromer-independent, ARF-6 dependent manner...
2017: PloS One
https://www.readbyqxmd.com/read/28701344/syp1-regulates-the-clathrin-mediated-and-clathrin-independent-endocytosis-of-multiple-cargo-proteins-through-a-novel-sorting-motif
#7
Amanda Reider Apel, Kyle Hoban, Silvia Chuartzman, Raffi Tonikian, Sachdev Sidhu, Maya Schuldiner, Beverly Wendland, Derek Prosser
Internalization of proteins from the plasma membrane (PM) allows for cell-surface composition regulation, signaling network modulation, and nutrient uptake. Clathrin-mediated endocytosis (CME) is a major internalization route for PM proteins. During CME, endocytic adaptor proteins bind cargos at the cell surface and link them to the PM and clathrin coat. Muniscins are a conserved family of endocytic adaptors, including Syp1 in budding yeast and its mammalian ortholog, FCHo1. These adaptors bind cargo via a C-terminal ì-homology domain (ìHD); however, few cargos exhibiting muniscin-dependent endocytosis have been identified, and the sorting sequence recognized by the μHD is unknown...
July 12, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28698057/membrane-trafficking-in-plant-immunity
#8
REVIEW
Yangnan Gu, Raul Zavaliev, Xinnian Dong
Plants employ sophisticated mechanisms to interact with pathogenic as well as beneficial microbes. Of those, membrane trafficking is key in establishing the rapid and precise response. Upon interaction with pathogenic microbes, surface-localized immune receptors undergo endocytosis for signal transduction and activity regulation while cell wall components, antimicrobial compounds, and defense proteins are delivered to pathogen invasion sites through polarized secretion. To sustain mutualistic associations, host cells also reprogram the membrane trafficking system to accommodate invasive structures of symbiotic microbes...
July 8, 2017: Molecular Plant
https://www.readbyqxmd.com/read/28697973/traffic-jam-hypothesis-relationship-between-endocytic-dysfunction-and-alzheimer-s-disease
#9
REVIEW
Nobuyuki Kimura, Katsuhiko Yanagisawa
Membrane trafficking pathways, like the endocytic pathway, carry out fundamental cellular processes that are essential for normal functioning. One such process is regulation of cell surface receptor signaling. A growing body of evidence suggests that β-amyloid protein (Aβ) plays a key role in Alzheimer's disease (AD) pathogenesis. Cleavage of Aβ from its precursor, β-amyloid precursor protein (APP), occurs through the endocytic pathway in neuronal cells. In early-stage AD, intraneuronal accumulation of abnormally enlarged endosomes is common, indicating that endosome trafficking is disrupted...
July 8, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28689729/trpml1-the-ca-2-retaker-of-the-lysosome
#10
REVIEW
Simone Di Paola, Anna Scotto-Rosato, Diego Luis Medina
Efficient functioning of lysosome is necessary to ensure the correct performance of a variety of intracellular processes such as degradation of cargoes coming from the endocytic and autophagic pathways, recycling of organelles, and signaling mechanisms involved in cellular adaptation to nutrient availability. Mutations in lysosomal genes lead to more than 50 lysosomal storage disorders (LSDs). Among them, mutations in the gene encoding TRPML1 (MCOLN1) cause Mucolipidosis type IV (MLIV), a recessive LSD characterized by neurodegeneration, psychomotor retardation, ophthalmologic defects and achlorhydria...
June 24, 2017: Cell Calcium
https://www.readbyqxmd.com/read/28674108/reciprocal-regulation-of-signaling-and-endocytosis-implications-for-the-evolving-cancer-cell
#11
REVIEW
Sandra L Schmid
Cell surface receptor uptake via clathrin-mediated endocytosis (CME) and subsequent intracellular sorting for degradation or recycling regulates the strength and specificity of downstream signaling. Signaling, in turn, modulates early endocytic trafficking. This reciprocal regulation of signaling and endocytosis provides opportunities for the establishment of feedback loops to enhance or suppress surface-derived signals. Recent studies suggest that dynamin-1, a presumed neuron-specific isoform of the large, membrane fission GTPase, can be activated in nonneuronal cells downstream of cancer-relevant signaling pathways and thereby function as a nexus between signaling and early endocytic trafficking...
July 3, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28656962/negative-regulation-of-egfr-signalling-by-the-human-folliculin-tumour-suppressor-protein
#12
Laura A Laviolette, Julien Mermoud, Isabel A Calvo, Nicholas Olson, Myriam Boukhali, Ortrud K Steinlein, Elisabeth Roider, Elke C Sattler, Dachuan Huang, Bin Tean Teh, Mo Motamedi, Wilhelm Haas, Othon Iliopoulos
Germline mutations in the Folliculin (FLCN) tumour suppressor gene result in fibrofolliculomas, lung cysts and renal cancers, but the precise mechanisms of tumour suppression by FLCN remain elusive. Here we identify Rab7A, a small GTPase important for endocytic trafficking, as a novel FLCN interacting protein and demonstrate that FLCN acts as a Rab7A GTPase-activating protein. FLCN(-/-) cells display slower trafficking of epidermal growth factor receptors (EGFR) from early to late endosomes and enhanced activation of EGFR signalling upon ligand stimulation...
June 28, 2017: Nature Communications
https://www.readbyqxmd.com/read/28650977/leishmania-donovani-resides-in-modified-early-endosomes-by-upregulating-rab5a-expression-via-the-downregulation-of-mir-494
#13
Jitender Kumar Verma, Ruchir Rastogi, Amitabha Mukhopadhyay
Several intracellular pathogens arrest the phagosome maturation in the host cells to avoid transport to lysosomes. In contrast, the Leishmania containing parasitophorous vacuole (PV) is shown to recruit lysosomal markers and thus Leishmania is postulated to be residing in the phagolysosomes in macrophages. Here, we report that Leishmania donovani specifically upregulates the expression of Rab5a by degrading c-Jun via their metalloprotease gp63 to downregulate the expression of miR-494 in THP-1 differentiated human macrophages...
June 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28650849/the-ins-and-outs-of-endocytic-trafficking-in-platelet-functions
#14
Meenakshi Banerjee, Sidney W Whiteheart
PURPOSE OF REVIEW: Although platelet endocytosis has been recognized in granule cargo loading and the trafficking of several platelet surface receptors, its acute physiological relevance is poorly understood as is its mechanism. The present review discusses the current understanding of platelet endocytosis and its implications for platelet function. RECENT FINDINGS: Recent studies are beginning to identify and define the proteins that mediate platelet endocytosis...
June 24, 2017: Current Opinion in Hematology
https://www.readbyqxmd.com/read/28640985/the-interaction-between-progranulin-and-prosaposin-is-mediated-by-granulins-and-the-linker-region-between-saposin-b-and-c
#15
Xiaolai Zhou, Peter M Sullivan, Lirong Sun, Fenghua Hu
The frontotemporal lobar degeneration (FTLD) protein progranulin (PGRN) is essential for proper lysosomal function. PGRN localizes in the lysosomal compartment within the cell. Prosaposin (PSAP), the precursor of lysosomal saposin activators (saposin A, B, C, D), physically interacts with PGRN. Previously we have shown that PGRN and PSAP facilitate each other's lysosomal trafficking. Here we report that the interaction between PSAP and PGRN requires the linker region of saposin B and C (BC linker). PSAP protein with the BC linker mutated fails to interact with PGRN and target PGRN to lysosomes in the biosynthetic and endocytic pathways...
June 22, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28634349/synuclein-impairs-trafficking-and-signaling-of-bdnf-in-a-mouse-model-of-parkinson-s-disease
#16
Fang Fang, Wanlin Yang, Jazmin B Florio, Edward Rockenstein, Brian Spencer, Xavier M Orain, Stephanie X Dong, Huayan Li, Xuqiao Chen, Kijung Sung, Robert A Rissman, Eliezer Masliah, Jianqing Ding, Chengbiao Wu
Recent studies have demonstrated that hyperphosphorylation of tau protein plays a role in neuronal toxicities of α-synuclein (ASYN) in neurodegenerative disease such as familial Alzheimer's disease (AD), dementia with Lewy bodies (DLB) and Parkinson's disease. Using a transgenic mouse model of Parkinson's disease (PD) that expresses GFP-ASYN driven by the PDGF-β promoter, we investigated how accumulation of ASYN impacted axonal function. We found that retrograde axonal trafficking of brain-derived neurotrophic factor (BDNF) in DIV7 cultures of E18 cortical neurons was markedly impaired at the embryonic stage, even though hyperphosphorylation of tau was not detectable in these neurons at this stage...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28632996/taking-control-hijacking-of-rab-gtpases-by-intracellular-bacterial-pathogens
#17
Stefania Spanò, Jorge E Galán
Intracellular bacterial pathogens survive and replicate within specialized eukaryotic cell organelles. To establish their intracellular niches these pathogens have adopted sophisticated strategies to control intracellular membrane trafficking. Since Rab-family GTPases are critical regulators of endocytic and secretory membrane trafficking events, many intracellular pathogens have evolved specific mechanisms to modulate or hijack Rab GTPases dynamics and trafficking functions. One such strategy is the delivery of bacterial effectors through specialized machines to specifically target Rab GTPases...
June 20, 2017: Small GTPases
https://www.readbyqxmd.com/read/28592304/post-translational-changes-to-%C3%AE-synuclein-control-iron-and-dopamine-trafficking-a-concept-for-neuron-vulnerability-in-parkinson-s-disease
#18
REVIEW
James A Duce, Bruce X Wong, Hannah Durham, Jean-Christophe Devedjian, David P Smith, David Devos
Parkinson's disease is a multifactorial neurodegenerative disorder, the aetiology of which remains elusive. The primary clinical feature of progressively impaired motor control is caused by a loss of midbrain substantia nigra dopamine neurons that have a high α-synuclein (α-syn) and iron content. α-Syn is a neuronal protein that is highly modified post-translationally and central to the Lewy body neuropathology of the disease. This review provides an overview of findings on the role post translational modifications to α-syn have in membrane binding and intracellular vesicle trafficking...
June 7, 2017: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/28591131/visualization-of-early-influenza-a-virus-trafficking-in-human-dendritic-cells-using-sted-microscopy
#19
Faezzah Baharom, Oliver S Thomas, Rico Lepzien, Ira Mellman, Cécile Chalouni, Anna Smed-Sörensen
Influenza A viruses (IAV) primarily target respiratory epithelial cells, but can also replicate in immune cells, including human dendritic cells (DCs). Super-resolution microscopy provides a novel method of visualizing viral trafficking by overcoming the resolution limit imposed by conventional light microscopy, without the laborious sample preparation of electron microscopy. Using three-color Stimulated Emission Depletion (STED) microscopy, we visualized input IAV nucleoprotein (NP), early and late endosomal compartments (EEA1 and LAMP1 respectively), and HLA-DR (DC membrane/cytosol) by immunofluorescence in human DCs...
2017: PloS One
https://www.readbyqxmd.com/read/28586196/real-time-imaging-of-endocytosis-and-intracellular-trafficking-of-semiconducting-polymer-dots
#20
Yuping Han, Xiaoming Li, Haobin Chen, Xingjie Hu, Yao Luo, Ting Wang, Zejun Wang, Qian Li, Chunhai Fan, Jiye Shi, Lihua Wang, Yun Zhao, Changfeng Wu, Nan Chen
Semiconducting polymer dots (Pdots) have shown great promise in biomedical applications, including biosensing, drug delivery, and live imaging of cells and biomolecules. Insight into the mechanism and regulation of cellular uptake and intracellular metabolism of Pdots is important for the development of superior Pdots-based theranostic nanoconjugates. Herein, we performed real-time imaging of endocytosis and intracellular trafficking of a type of fluorescent Pdots that showed excellent biocompatibility in various types of cells...
June 13, 2017: ACS Applied Materials & Interfaces
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