Zilu Wang, Fengxin Cui, Yinuo Chen, Hongyun Liu, Yiran Zhang, Yaxin Shi, Yinuo Zhang, Yanfei Wang, Na Liang, Li Xu, Ying Liu, Zhiyong Liu, Wei Shi
Because adenomyosis (AM) ectopic primary cells are hard to come by, have a short lifespan, and the characteristics that alter over time, their utility in AM research is constrained. This study aimed to establish a line of immortalized human adenomyosis ectopic cell (ihAMEC) to change this situation. Primary cells were obtained from AM ectopic lesion tissue and then infected with Simian Vacuolating Virus 40 Tag (SV40 T) lentivirus and screened to establish immortalized cells. We verified the main features and found that the ihAMEC could be cultured for more than 50 generations and the proliferation ability of ihAMEC was more active than that of primary cells...
December 1, 2023: Tissue & Cell