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https://www.readbyqxmd.com/read/29129333/pitfalls-in-the-interpretation-of-specimens-from-patients-with-testicular-tumours-with-an-emphasis-on-variant-morphologies
#1
REVIEW
Thomas M Ulbright
Accurate diagnosis of primary and metastatic tumours is essential in testicular cancer. While many cases are straightforward, some pose difficulties, especially when variant morphologies occur. Seminoma with 'atypical' features, including increased nuclear pleomorphism and crowding and greater cytoplasmic density with loss of membrane definition, mimics embryonal carcinoma, although ancillary features (fibrous septa, lymphocytes) and immunohistochemistry are of great help. Other deceptive seminoma features include prominent to exclusive intertubular growth, microcystic/tubular patterns, and signet-ring tumour cells...
November 9, 2017: Pathology
https://www.readbyqxmd.com/read/29097843/a-case-report-of-ventricular-septal-defect-in-an-adult-sprague-dawley-rat
#2
Tetsuro Kurotaki, Yuki Tomonari, Junko Sato, Takeshi Kanno, Minoru Tsuchitani
A 20-week-old male Sprague Dawley rat noted with decreased body weight, dyspnea, and anorexia beginning 2 days before death was necropsied in the recovery period of a sub-chronic toxicity study. The heart was severely enlarged (30 × 20 × 20 mm), 3-4 times larger than normal, with an approximately 6 mm wide defect in the upper, membranous portion of the ventricular septum. Both ventricles measured 4 mm in thickness, and the right ventricle was 4 times thicker than normal. According to a microscopic examination, the myocardial fibers were severely hypertrophic in the right ventricle and mildly hypertrophic in the left ventricle and septum...
October 2017: Journal of Toxicologic Pathology
https://www.readbyqxmd.com/read/29097837/two-types-of-deposits-hyaline-droplets-and-eosinophilic-bodies-associated-with-%C3%AE-2u-globulin-accumulation-in-the-rat-kidney
#3
Masao Hamamura, Takafumi Oshikata, Koshirou Katoku, Minoru Tsuchitani, Ryoji Yamaguchi
Alpha2u-globulin is an adult male rat-specific protein that accumulates spontaneously or inductively in the renal proximal tubular epithelium and forms microscopically observable deposits, which are generally referred to as "hyaline droplets," whereas a specific type of deposits is referred to as "eosinophilic bodies" by Japanese toxicologic pathologists. We compared hyaline droplets and eosinophilic bodies using special stains including immunostaining for α2u-globulin and lysosome-associated membrane protein in spontaneously occurring and d-limonene-induced cases...
October 2017: Journal of Toxicologic Pathology
https://www.readbyqxmd.com/read/29095742/holocrine-poroma-a-distinctive-adnexal-tumor
#4
Marcelo G Horenstein, Marina P Sandoval, Yungtai Lo, Jack Jacob
There are 36 cases of complex poroid tumors with folliculosebaceous and apocrine differentiation reported in the literature. The authors evaluated 111 poroid tumors including 63 typical eccrine poromas and 48 poroid tumors with folliculosebaceous elements. Folliculosebaceous poroid tumors (FSPT) had basaloid and squamous cells (100%), ducts with steatocystoma-like cuticles and holocrine secretions (89.6%), infundibular follicular structures (66.7%), and entrapped sebocytes (56.3%). No definite apocrine decapitation secretions in FSPT were found...
November 1, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/29072602/isolation-and-functional-characterization-of-an-acidic-myotoxic-phospholipase-a%C3%A2-from-colombian-bothrops-asper-venom
#5
Silvia Posada Arias, Paola Rey-Suárez, Andrés Pereáñez J, Cristian Acosta, Mauricio Rojas, Lucilene Delazari Dos Santos, Rui Seabra Ferreira, Vitelbina Núñez
Myotoxic phospholipases A₂ (PLA₂) are responsible for many clinical manifestations in envenomation by Bothrops snakes. A new myotoxic acidic Asp49 PLA₂ (BaCol PLA₂) was isolated from Colombian Bothrops asper venom using reverse-phase high performance liquid chromatography (RP-HPLC). BaCol PLA₂ had a molecular mass of 14,180.69 Da (by mass spectrometry) and an isoelectric point of 4.4. The complete amino acid sequence was obtained by cDNA cloning (GenBank accession No. MF319968) and revealed a mature product of 124 amino acids with Asp at position 49...
October 26, 2017: Toxins
https://www.readbyqxmd.com/read/29066477/carcinoma-in-situ-involving-sclerosing-adenosis-seeking-the-salient-histological-characteristics-to-prevent-overdiagnosis
#6
Xiaoyan Cui, Shi Wei
Ductal or lobular carcinoma in situ (DCIS/LCIS) can rarely arise from sclerosing adenosis (SA). The combination of cytologically malignant cells and the infiltrative growth pattern may make it challenging to distinguish it from an invasive carcinoma. The authors reviewed 50 consecutive cases of CIS involving SA to seek the salient histologic characteristics in order to prevent overdiagnosis. The features commonly seen with CIS were the lobular configuration at low magnification (94%), uninvolved SA in neighboring tissue (84%), at least focally identifiable myoepithelial cells on H&E-stained sections (76%), separate foci of unequivocal CIS (58%), associated microcalcifications (54%), and hyaline basement membrane surrounding tumor cell nests (48%)...
September 2017: Annals of Clinical and Laboratory Science
https://www.readbyqxmd.com/read/29062187/acute-toxicity-of-commercial-atrazine-in-piaractus-mesopotamicus-histopathological-ultrastructural-molecular-and-genotoxic-evaluation
#7
Paula Pereira de Paiva, Mariana Cruz Delcorso, Valquíria Aparecida Matheus, Sonia Claudia do Nascimento de Queiroz, Carla Beatriz Collares-Buzato, Sarah Arana
AIM: The aim of this work was to evaluate the sensitivity of Pacu fingerlings (Piaractus mesopotamicus) by measuring the effects of median lethal concentration (LC50) of atrazine (ATZ - 28.58 mg/L) after acute exposure (up to 96 h). MATERIALS AND METHODS: The fish were exposed to the LC50 of ATZ for 96 h (28.58 mg/L) in a static system. During the experiment, the fingerlings were randomly distributed in four glass tanks (50 L) containing dechlorinated water. Four glass tanks were for the control group, and four were for the ATZ-exposed group (n=4 per glass tank), given a total number of 16 animals tested per group...
September 2017: Veterinary World
https://www.readbyqxmd.com/read/28987651/ultrastructure-and-phylogeny-of-kirithra-asteri-gen-et-sp-nov-ceratoperidiniaceae-dinophyceae-a-free-living-thin-walled-marine-photosynthetic-dinoflagellate-from-argentina
#8
Pernille Vængebjerg Boutrup, Øjvind Moestrup, Urban Tillmann, Niels Daugbjerg
A gymnodinioid photosynthetic dinoflagellate was isolated from Argentina and examined by light and electron microscopy and analysis of nuclear-encoded LSU rDNA. Kirithra asteri gen. et sp. nov. was proposed as morphology and molecular phylogeny separated this dinoflagellate from others within the family Ceratoperidiniaceae. Cells were surrounded by a hyaline amphiesma comprising polygonal vesicles. Each vesicle contained a honeycomb and a trilaminar structure. An anterior sulcal extension ending in a complete circle formed the apical structure complex (ASC), which characterizes Ceratoperidiniaceae...
September 9, 2017: Protist
https://www.readbyqxmd.com/read/28941146/tracheal-repair-with-acellular-human-amniotic-membrane-in-a-rabbit-model
#9
Lianna Ferrari Jorge, Julio C Francisco, Nelson Bergonse, Cristina Baena, Katherine Athayde T Carvalho, Eltyeb Abdelwahid, Jose Rocha Faria Neto, Luiz Felipe Pinho Moreira, Luiz Cesar Guarita-Souza
BACKGROUND: Surgical correction of tracheal stenosis is still a complex and challenging procedure. Acellular human amniotic membranes (AHAM) represent a promising biomaterial source for tissue regeneration. The aim of this study was to evaluate whether AHAM grafts improve tissue regeneration of the trachea in a rabbit model of tracheostomy. METHODS: Twenty rabbits were randomized into two groups. Animals in the control group underwent surgical tracheostomy only and animals in the AHAM group underwent surgical tracheostomy and received an AHAM graft that covered the defect site...
September 22, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28881049/classical-gonadoblastoma-its-relationship-to-the-dissecting-variant-and-undifferentiated-gonadal-tissue
#10
REVIEW
Lawrence M Roth, Liang Cheng
Classical gonadoblastoma occurs almost entirely in the dysgenetic gonads of an individual who has a disorder of sex development. Approximately 40% of such neoplasms are bilateral. Almost all gonadoblastomas occur in patients who have a Y chromosome or part thereof; testis specific protein Y-encoded 1 (TSPY1) is the putative gene. If a gonad in a patient who has a disorder of sex development contains germ cells with delayed maturation and also harbors the TSPY1 gene, the cells can undergo transformation to classical gonadoblastoma...
September 7, 2017: Histopathology
https://www.readbyqxmd.com/read/28872083/-repair-of-articular-cartilage-defects-by-autologous-bone-mesenchymal-stem-cells-and-allogeneic-costal-chondrocytes-in-the-knee-of-wuzhishan-miniature-pigs
#11
Cheng Yang, Jiangdong Ni, Shou Zhang, Zhongcheng Fan
To investigate the feasibility of construction of tissue engineered cartilage by co-culture of bone marrow mesenchymal stem cells (BMSCs) and costal chondrocytes (CCs), and to provide theoretical basis and experimental basis for clinical repair of articular cartilage defects by Wuzhishan miniature pig knee cartilage defects with co-cultured cells.
 Methods: Density gradient centrifugation method was used to isolate BMSCs from Wuzhishan miniature pig. The double enzyme digestion method was used to isolate CCs...
August 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28813619/age-related-changes-in-the-chorioretinal-junction-an-immunohistochemical-study
#12
Tulika Gupta, Narbada Saini, Jasbir Arora, Daisy Sahni
The chorioretinal junction comprises the retinal pigment epithelium, Bruch's membrane (BM), and adjacent choroidal capillaries. Its significance lies in its ability to support the retina mechanically and metabolically. The aim of this cross-sectional study was to record the senescent changes affecting all the constituents of the chorioretinal junction in 40 histological specimens across the whole spectrum of the adult age range. This study included light microscopy, with hematoxylin and eosin and PAS stains, and fluorescent microscopy...
October 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28807065/possible-role-of-dickkopf-1-protein-in-the-pathogenesis-of-tympanosclerosis-in-a-rat-model
#13
Y Zhang, S Wang, Y Zheng, A Liu
OBJECTIVES: This study aimed to investigate the expression of DKK1 protein in an experimental model of tympanosclerosis and its possible role in the pathogenesis of this disorder. METHODS: Forty Sprague Dawley rats were included in the study: 20 in the control group (which received no treatment) and 20 in the experimental group (which received an incision to induce tympanosclerosis). Otomicroscopy was performed to observe the development of myringosclerosis. Haematoxylin and eosin staining was performed to observe the morphological changes...
October 2017: Journal of Laryngology and Otology
https://www.readbyqxmd.com/read/28805143/a-pre-term-infant-of-32-weeks-gestation-with-congenital-tuberculosis-treated-successfully-with-antituberculosis-chemotherapy
#14
Xiaoyi Fang, Ruizhi Mai, Jizhong Guo, Niyang Lin
An infant of 32 weeks gestation was separated from her mother at birth for treatment of hyaline membrane disease and, on recovery, was cared for by adoptive parents. At 25 days, she was treated for pneumonia with immunoglobulins and multiple antibiotics and appeared to respond. Her symptoms recurred at 8 weeks and tuberculosis was confirmed by detection in an acid-fast bacilli smear of gastric aspirate. Her mother presented with disseminated tuberculosis with meningitis 1 month after delivery. Criteria for the diagnosis of congenital tuberculosis in the infant were confirmed...
August 14, 2017: Paediatrics and International Child Health
https://www.readbyqxmd.com/read/28794361/nivolumab-induced-acute-fibrinous-and-organizing-pneumonia-afop
#15
Tsukasa Ishiwata, Takahiro Ebata, Shunichiro Iwasawa, Jun Matsushima, Satoshi Ota, Yukio Nakatani, Kenji Tsushima, Yuji Tada, Koichiro Tatsumi, Yuichi Takiguchi
Although nivolumab is known to cause immune-related interstitial lung diseases (ILD), the detailed characteristics of ILD are still not fully understood. A 68-year-old man was treated with nivolumab because of unresectable sinonasal melanoma, he achieved a complete response soon after the initiation of the therapy and a complete response was thereafter maintained for 30 weeks until the patient experienced dyspnea of subacute onset. CT images revealed patchy infiltrates and ground-glass opacifications. The bronchoalveolar lavage fluid (BALF) contained elevated percentages of lymphocytes (53%) and neutrophils (30%)...
September 1, 2017: Internal Medicine
https://www.readbyqxmd.com/read/28764188/cytology-of-collagenous-spherulosis-a-case-series
#16
Devi Subbarayan, Vijayashree Raghavan
Collagenous Spherulosis (CS) is an uncommon benign entity characterized by the presence of pink hyaline globules composed of basement membrane like material. Although they are known to occur independently, they are more often found as incidental findings in association with benign pro iferative breast lesions. As morphologically similar hyaline globules also occur in certain malignant lesions such as Adenoid Cystic Carcinoma (ACC) and adenomyoepithelial carcinoma, their presence may pose diagnostic problems...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28734808/-congenital-cytomegalovirus-infection-manifesting-as-neonatal-respiratory-distress-in-an-hiv-exposed-uninfected-newborn
#17
A Pham, H El Mjati, N Nathan, F Kieffer, D Mitanchez
BACKGROUND: Cytomegalovirus (CMV) is one of the most common intrauterine infections, affecting approximately 1% of all live births. There are few reports on congenital CMV infections manifesting as isolated pneumonitis. CASE REPORT: We report a case of congenital CMV with neonatal respiratory distress affecting an HIV-exposed uninfected infant. This infant required noninvasive ventilation beginning within the first 15min of life. The initial chest X-ray showed diffuse bilateral ground-glass opacifications...
July 19, 2017: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/28721683/mesenchymal-stem-progenitor-cells-derived-from-articular-cartilage-synovial-membrane-and-synovial-fluid-for-cartilage-regeneration-current-status-and-future-perspectives
#18
REVIEW
Yi-Zhou Huang, Hui-Qi Xie, Antonietta Silini, Ornella Parolini, Yi Zhang, Li Deng, Yong-Can Huang
Large articular cartilage defects remain an immense challenge in the field of regenerative medicine because of their poor intrinsic repair capacity. Currently, the available medical interventions can relieve clinical symptoms to some extent, but fail to repair the cartilaginous injuries with authentic hyaline cartilage. There has been a surge of interest in developing cell-based therapies, focused particularly on the use of mesenchymal stem/progenitor cells with or without scaffolds. Mesenchymal stem/progenitor cells are promising graft cells for tissue regeneration, but the most suitable source of cells for cartilage repair remains controversial...
October 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28665930/kidneys-in-5-year-old-preterm-born-children-a-longitudinal-cohort-monitoring-of-renal-function
#19
Rachel Vieux, Marion Gerard, Audrey Roussel, Aïssata Sow, Amelie Gatin, Francis Guillemin, Jean-Michel Hascoet
BackgroundBeing aware of the impact of low birth weight on late-onset hypertension, our aim was to describe systolic blood pressure (sBP) and renal function in 3-5-year-old preterm-born children and to determine which perinatal factors or childhood factors were associated with an altered renal function at 5 years in these children.MethodsThis was a prospective longitudinal cohort study of children born at 27-31 weeks of gestation and included at birth and examined at 3, 4, and 5 years of age. The primary outcome was renal function at 5 years: BP, estimated glomerular filtration rate, and albuminuria...
December 2017: Pediatric Research
https://www.readbyqxmd.com/read/28661210/solid-pseudopapillary-neoplasm-of-the-pancreas-a-rare-entity-with-unique-features
#20
REVIEW
Peyman Dinarvand, Jinping Lai
Solid pseudopapillary neoplasm of the pancreas is a rare entity with low malignant potential and excellent overall prognosis. It has nonspecific clinical presentations such as abdominal pain and nausea, with vague radiologic features. Histologic features of this neoplasm are usually specific. The tumor shows minimally cohesive, uniform, monotonous cells lining delicate capillary-sized blood vessels, described as pseudopapillary architecture. Other features including hyaline globules, cytoplasmic vacuoles, and nuclear grooving are frequently present...
July 2017: Archives of Pathology & Laboratory Medicine
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