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Giovanna Squintani, Francesco Donato, Mara Turri, Luciano Deotto, Francesco Teatini, Giuseppe Moretto, Roberto Erro
BACKGROUND: Delta-9-tetrahydrocannabinol and cannabidiol (THC:CBD) oromucosal spray (Sativex®) has been recently approved for the management of treatment-resistant multiple sclerosis (MS) spasticity. Although the symptomatic relief of Sativex® on MS-spasticity has been consistently demonstrated, the pathogenetic implications remain unclear and the few electrophysiological studies performed to address this topic yielded controversial results. We therefore aimed to investigate the mechanisms underpinning the modulation of spastic hypertonia by Sativex®, at both central and spinal levels, through an extensive neurophysiological battery in patients with MS...
November 15, 2016: Journal of the Neurological Sciences
Fabian Lorenzo-Diaz, Cris Fernández-Lopez, Pierre-Emmanuel Douarre, Adrian Baez-Ortega, Carlos Flores, Philippe Glaser, Manuel Espinosa
Streptococcus agalactiae or Group B Streptococcus (GBS) are opportunistic bacteria that can cause lethal sepsis in children and immuno-compromised patients. Their genome is a reservoir of mobile genetic elements that can be horizontally transferred. Among them, integrative and conjugative elements (ICEs) and the smaller integrative and mobilizable elements (IMEs) primarily reside in the bacterial chromosome, yet have the ability to be transferred between cells by conjugation. ICEs and IMEs are therefore a source of genetic variability that participates in the spread of antibiotic resistance...
October 2016: Open Biology
Aiste McCormick, Peter Donoghue, Michelle Dixon, Richard O'Sullivan, Rachel Louise O'Donnell, James Murray, Angelika Kaufmann, Nicola J Curtin, Richard J Edmondson
PURPOSE: DNA damage defects are common in ovarian cancer and can be used to stratify treatment. Although most work has focussed on Homologous Recombination (HR), DNA double strand breaks are repaired primarily by non-homologous end joining (NHEJ). Defects in NHEJ have been shown to contribute to genomic instability and have been associated with the development of chemoresistance. EXPERIMENTAL DESIGN: NHEJ was assessed in a panel of ovarian cancer cell lines and 47 primary ascitic derived ovarian cancer cultures, by measuring the ability of cell extracts to end-join linearized plasmid monomers into multimers...
October 4, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Po-Ru Loh, Petr Danecek, Pier Francesco Palamara, Christian Fuchsberger, Yakir A Reshef, Hilary K Finucane, Sebastian Schoenherr, Lukas Forer, Shane McCarthy, Goncalo R Abecasis, Richard Durbin, Alkes L Price
Haplotype phasing is a fundamental problem in medical and population genetics. Phasing is generally performed via statistical phasing in a genotyped cohort, an approach that can yield high accuracy in very large cohorts but attains lower accuracy in smaller cohorts. Here we instead explore the paradigm of reference-based phasing. We introduce a new phasing algorithm, Eagle2, that attains high accuracy across a broad range of cohort sizes by efficiently leveraging information from large external reference panels (such as the Haplotype Reference Consortium; HRC) using a new data structure based on the positional Burrows-Wheeler transform...
October 3, 2016: Nature Genetics
Chang Ha Park, Young Seon Kim, Xiaohua Li, Haeng Hoon Kim, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Sook Young Lee, Sang Un Park
Carbohydrate sources play important roles in energy and growth of plants. Therefore, in this study, we investigated the optimal carbohydrate source in hairy root cultures (HRCs) of Scutellaria baicalensis infected with Agrobacterium rhizogenes strain R1000. The hairy roots were cultured in half-strength B5 liquid medium supplemented with seven different carbohydrates sources (sucrose, fructose, glucose, galactose, sorbitol, mannitol and maltose), each at a concentration of 100 mM, in order to identify the best carbon sources for the production of major flavones, such as wogonin, baicalin and baicalein...
June 2016: Natural Product Communications
Domingo J Ramos-Campo, Jacobo A Rubio-Arias, Tomás Freitas, Arturo Camacho, José Fernando Jiménez-Diaz, Pedro E Alcaraz
The aim of this study was to analyze physical performance and physiological variables during High-Intensity Resistance Circuit Training (HRC) with the addition of two levels (moderate and high) of systemic hypoxia. Twelve resistance-trained young male subjects participated in the study. After a 6RM testing session, participants performed three randomized trials of HRC: normoxia (NORM: FiO2=0.21; ∼0 m altitude), moderate hypoxia (MH: FiO2=0.16; ∼2.100 m altitude) or high hypoxia (HH: FiO2=0.13; ∼3.800 m altitude), as controlled by a hypoxic generator...
July 19, 2016: Journal of Strength and Conditioning Research
E I Dorokhina, A U Magomedova, V N Dvirnyk, I V Gal'tseva, S A Glinkina, S M Kulikov, T N Obukhova, S K Kravchenko
AIM: to evaluate late myelotoxicity (MT) relate to high-dose chemotherapy (CT) according to the modified NHL-BFM-90 (mNHL-BFM-90) program in adult patients with diffuse large B-cell lymphoma (DLBCL). SUBJECTS AND METHODS: The results of a complex clinical, laboratory, and instrumental examination, including cytologic, histologic, and routine cytogenetic studies of the bone marrow (BM), were analyzed in 40 DLBCL patients treated according to the mNHL-BFM-90 program in the National Research Center for Hematology (NRCH), Ministry of Health of the Russian Federation (MHRF), in 2002 to 2009; among them, there were 20 men and 20 women (median age, 57 years)...
2016: Terapevticheskiĭ Arkhiv
Brynne A Sullivan, Christina McClure, Jamie Hicks, Douglas E Lake, J Randall Moorman, Karen D Fairchild
OBJECTIVES: To determine whether an early heart rate characteristics (HRC) index (HeRO score), measured in the first day and week after birth predicts death and morbidities compared with established illness severity scores. STUDY DESIGN: For all very low birth weight infants in a single neonatal intensive care unit from 2004-2014, the average first day HRC index was calculated within 24 hours of birth (aHRC-24h) and the average first week HRC index within 7 days of birth (aHRC-7d)...
July 2016: Journal of Pediatrics
Toru Kondo, Masahiro Matsuoka, Chihiro Azai, Shigeru Itoh, Hirozo Oh-Oka
Orientations of the FA and FB iron-sulfur (FeS) clusters in a structure-unknown type-I homodimeric heriobacterial reaction center (hRC) were studied in oriented membranes of the thermophilic anaerobic photosynthetic bacterium Heliobacterium modesticaldum by electron paramagnetic resonance (EPR), and compared with those in heterodimeric photosystem I (PS I). The Rieske-type FeS center in the cytochrome b/c complex showed a well-oriented EPR signal. Illumination at 14 K induced an FB(-) signal with g-axes of gz = 2...
May 12, 2016: Journal of Physical Chemistry. B
Jing Li, Jun Zhang, Minghui Tang, Junping Xin, Yan Xu, Andrew Volk, Caiqin Hao, Chenglong Hu, Jiewen Sun, Wei Wei, Quichan Cao, Peter Breslin, Jiwang Zhang
The phosphorylated form of Pten (p-Pten) is highly expressed in >70% of acute myeloid leukemia samples. However, the role of p-Pten in normal and abnormal hematopoiesis has not been studied. We found that Pten protein levels are comparable among long-term (LT) hematopoietic stem cells (HSCs), short-term (ST) HSCs, and multipotent progenitors (MPPs); however, the levels of p-Pten are elevated during the HSC-to-MPP transition. To study whether p-Pten is involved in regulating self-renewal and differentiation in HSCs, we compared the effects of overexpression of p-Pten and nonphosphorylated Pten (non-p-Pten) on the hematopoietic reconstitutive capacity (HRC) of HSCs...
August 2016: Stem Cells
Alfonso Pitarque, Juan Meléndez, Alicia Sales, Teresa Mayordomo, Joaquín Escudero, Salvador Algarabel
We present an associative recognition experiment comparing three samples of healthy people (young people, older people with high cognitive reserve [HCR], and older people with low cognitive reserve [LCR], with each sample consisting of 40 people), manipulating stimuli repetition during the study phase. The results show significant differences among the three samples in their overall performance. However, these differences are not due to a different use of familiarity, but rather due to a different way of using recollection: although there are no differences in the hit rates between the HRC and LRC samples, the LCR group makes significantly more recollective false alarms than the HCR group...
September 2016: Neuropsychology, Development, and Cognition. Section B, Aging, Neuropsychology and Cognition
Xiaofeng Zhou, Qing Yan, Nian Wang
Xanthomonas contains a large group of plant-associated species, and many of them cause severe diseases on important crops worldwide. Six gluconate-operon repressor (GntR) family transcriptional regulators are predicted in Xanthomonas and one of them belonging to the YtrA subfamily plays a prominent role in bacterial virulence. However, direct targets and comprehensive regulatory profile of YtrA remain unknown. Here, we performed microarray and high-resolution ChIP-exo experiment to identify YtrA direct targets and its DNA binding motif in X...
March 12, 2016: Molecular Plant Pathology
Fernando Pareja-Blanco, David Rodríguez-Rosell, Luis Sánchez-Medina, Juan Ribas-Serna, Covadonga López-López, Ricardo Mora-Custodio, Juan Manuel Yáñez-García, Juan José González-Badillo
This study compared the time course of recovery following two resistance exercise protocols differing in the number of repetitions per set with regard to the maximum possible (to failure) number. Ten men performed three sets of 6 versus 12 repetitions with their 70% 1RM (3 × 6 [12] versus 3 × 12 [12]) in the bench press (BP) and squat (SQ) exercises. Mechanical [CMJ height, velocity against the 1 m s(-1) load (V1 -load)], biochemical [testosterone, cortisol, growth hormone, prolactin, insulin-like growth factor-1, creatine kinase (CK)] and heart rate variability (HRV) and complexity (HRC) were assessed pre-, postexercise (Post) and at 6, 24 and 48 h-Post...
March 11, 2016: Clinical Physiology and Functional Imaging
E de Thomasson, S Gouzy, F Cloerec, L Geais
INTRODUCTION: Computer-assisted surgery improves the positioning of hip prostheses but requires use of transosseous pins requiring a complementary approach exposing the patient to rare but at times serious complications. The use of sensor arrays attached to the skin could advantageously replace pins provided that comparable results are obtained, but their validity has not yet been assessed. We conducted a prospective in vitro study to: measure the possible error of a cutaneous versus transosseous fixation to determine the hip rotation center (HRC) position and determine the inter- and intraobserver reproducibility of the cutaneous versus the transosseous fixation...
May 2016: Orthopaedics & Traumatology, Surgery & Research: OTSR
Belén Sanchez-Bridge, Mathieu Renouf, Julien Sauser, Maurice Beaumont, Lucas Actis-Goretta
Understanding the bioavailability and metabolism of coffee compounds will contribute to identify the unknown biological mechanism(s) linked to their beneficial effects. The influence of the roasting process on the metabolism of coffee chlorogenic acids in humans was evaluated. In a randomized, double-blind, crossover study, 12 healthy volunteers consumed four instant coffees namely, high roasted coffee (HRC), low roasted coffee (LRC), unroasted coffee (URC), and in vitro hydrolyzed unroasted coffee (HURC). The sum of areas under the curve (AUC) ranged from 8...
May 2016: BioFactors
Ali Foroutan, Shahram Paydar, Seyed Taghi Heydari, Gholamhussian Erjaee, Kamran Bagheri Lankarani, Abbas Nowroozi, Sam Moslemi
BACKGROUND: Nonlinear analysis of heart rate variability (HRV) has been recently used as a predictor of prognosis in trauma patients. OBJECTIVES: We applied nonlinear analysis of HRV in patients with blunt trauma and intraperitoneal bleeding to assess our ability to predict the outcome of conservative management. PATIENTS AND METHODS: An analysis of electrocardiography (ECG) from 120 patients with blunt trauma was conducted at the onset of admission to the emergency department...
November 2015: Trauma Monthly
Tomás T Freitas, Julio Calleja-González, Francisco Alarcón, Pedro E Alcaraz
This study aimed to investigate the acute effects of two different resistance circuit training protocols on basketball players' physical and technical performance and rating of perceived exertion (RPE). In a repeated-measures, crossover experimental design, 9 semiprofessional basketball players performed a Power Circuit Training (PCT; 45% 1RM) and a High-Resistance Circuit Training (HRC; 6RM), on consecutive weeks. Vertical and horizontal jump performance, 3-points shooting accuracy, repeated-sprint ability (RSA), agility, and upper body power output were measured before and after training...
February 2016: Journal of Strength and Conditioning Research
Hong-Lin Zhao, Fan Yang, Xin Huang, Qing-Hua Zhou
Angiogenesis is an indispensible process for tumor growth and metastasis. Anti-angiogenesis based therapy is one of the most promising treatments for inhibiting cancer progression. Through the exploration of inhibitors of vascular endothelial growth factor receptor (VEGFR)-2, deemed as the major angiogenesis pathway, pazopanib was found as a small molecular pan-VEGFR and pan-platelet-derived growth factor receptor (PDGFR) inhibitor, with suitable pharmacodynamic and pharmacokinetic parameters to be an oral drug...
November 2014: Thoracic Cancer
José Luis Zambrana-García, María Isabel Montoro-Caba, Maite Chicano-Gallardo, Ana Monrobel-Lancho, Daniel Jesús Pérez-de-Luque, José Antonio Peña-Ojeda, José Manuel Recio-Ramírez
BACKGROUND: By high resolution consultation (HRC) we mean an ambulatory process of assistance fulfilled in a single day, by which treatment and diagnosis are established and recorded. OBJECTIVE: To assess to which extent patients with digestive conditions may benefit from a single consultation system. MATERIAL: A descriptive study of 179 first visit events, randomly selected as high-resolution consultations in gastroenterology. We discuss the percentage of patients who benefited from HRC and the complementary tests performed...
January 2016: Revista Española de Enfermedades Digestivas
Paola A Spadaro, Helen L Naug, Eugene F DU Toit, Daniel Donner, Natalie J Colson
Consumption of palatable foods high in refined carbohydrate has been implicated as a contributing factor to the epidemic levels of obesity. Such foods may disrupt appetite regulation in the hypothalamus through alterations in hunger and satiety signalling. This investigation examined whether a palatable high refined carbohydrate (HRC) diet with the potential to induce obesity was linked to modulation of serotonin and dopamine signalling within the hypothalamus of rats. Male Wistar rats were allowed ad libitum access to either a palatable refined carbohydrate enriched (HRC) diet or standard chow (SC)...
2015: Genetics Research
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