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Süreyya Gül Yurtsever, Can Biçmen, Ayriz T Gündüz, Nuri Ozkütük, Serkan Salman, Mustafa Demirci
This study was conducted to compare BACTEC 460 TB system and the proportion method in commercially available and ready to use antibiotic added Löweinstein-Jensen (LJ) medium for susceptibility testing of first line drugs in Mycobacterium tuberculosis complex isolates. A total 238 M.tuberculosis strains isolated from clinical samples in our laboratory between 2006-2010 period were included in the study. Susceptibility testing for streptomycin, isoniazid, rifampicin and ethambutol in commercially provided LJ medium (Salubris Inc...
October 2011: Mikrobiyoloji Bülteni
Peter Daley, Joy Sarojini Michael, Kalaiselvan S, Asha Latha, Dilip Mathai, K R John, Madhukar Pai
BACKGROUND: Direct sputum smear microscopy for tuberculosis (TB) lacks sensitivity for the detection of acid fast bacilli. Sputum pretreatment procedures may enhance sensitivity. We did a pilot study to compare the diagnostic accuracy and incremental yield of two short-duration (<1 hour) sputum pretreatment procedures to optimize direct smears among patients with suspected TB at a referral hospital in India. METHODOLOGY/FINDINGS: Blinded laboratory comparison of bleach and universal sediment processing (USP) pretreated centrifuged auramine smears to direct Ziehl-Neelsen (ZN) and direct auramine smears and to solid (Loweinstein-Jensen (LJ)) and liquid (BACTEC 460) culture...
May 20, 2009: PloS One
Celia S Chen, Lindy Loweinstein, Craig James, Shyamala C Huilgol, Dinesh Selva
No abstract text is available yet for this article.
April 21, 2003: Medical Journal of Australia
R R Kazwala, C J Daborn, J M Sharp, D M Kambarage, S F Jiwa, N A Mbembati
SETTING: Pastoralist communities in the Northern and Southern zones of Tanzania. DESIGN: Observational study. OBJECTIVES: To determine the involvement of Mycobacterium bovis in tuberculosis cases presenting at tuberculosis (TB) clinics in rural areas in these zones. METHODS: A total of 149 tuberculosis cases identified on the bases of clinical manifestation were sampled. Appropriate specimens were cultured on two Löweinstein Jensen slants with respectively glycerol and pyruvate added...
January 2001: International Journal of Tuberculosis and Lung Disease
F Z Badak, S Goksel, R Sertoz, B Nafile, S Ermertcan, C Cavusoglu, A Bilgic
The feasibility of using nucleic acid probes directly from positive MB/BacT broth to identify mycobacteria was determined in this study. A total number of 2,727 specimens were cultured into the MB/BacT (Organon Teknika) automated system and on conventional Loweinstein-Jensen (LJ) slants. The Gen-Probe AccuProbe culture identification tests (DNA probes) were used on samples from bottles which were identified as positive for mycobacteria by MB/BacT. Samples of positive MB/BacT broth (0.1 ml) were used directly in the broth culture method for the DNA probes as published by Gen-Probe...
May 1999: Journal of Clinical Microbiology
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