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Mustafa O Jibrin, Neha Potnis, Sujan Timilsina, Gerald V Minsavage, Gary E Vallad, Pamela D Roberts, Jeffrey B Jones, Erica M Goss
Recombination is a major driver of evolution in bacterial populations because it can spread and combine independently evolved beneficial mutations. Recombinant lineages of plant bacterial pathogens are typically associated with colonization of novel hosts and emergence of new diseases. Here, we show that recombination between evolutionarily and phenotypically distinct plant pathogenic lineages generated recombinant lineages with unique combinations of pathogenicity and virulence factors. X. euvesicatoria ( Xe ) and X...
April 20, 2018: Applied and Environmental Microbiology
Ashish Jain, Sanchit Jain, Neha Singh, Priyanka Aswal, Shweta Pal, Sushant Kumar Meinia, Nilotpal Chowdhury
Introduction: This study aimed to investigate the analytical bias in haematological parameters induced by storage at 33 ºC. Materials and methods: Blood from the diversion pouch of 20 blood donors were collected in K 2 EDTA vials and stored at 33 ºC. Readings from each vial were taken at 0, 4, 6, 12, 24, 48 and 72 hours after collection on the Sysmex XP-100 analyser (Sysmex Corporation, Kobe, Japan). The percent difference from the baseline readings were calculated and subjected to a Wilcoxon signed rank test at a Holm corrected significance level of 0...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
M Gencay, A Seffner, S Pabinger, J Gautier, P Gohl, M Weizenegger, D Neofytos, R Batrla, A Woeste, H S Kim, G Westergaard, C Reinsch, E Brill, P T T Thuy, B H Hoang, M Sonderup, C W Spearman, G Brancaccio, M Fasano, G B Gaeta, T Santantonio, W E Kaminski
An important requirement for a state-of-the-art hepatitis B surface antigen (HBsAg) screening assay is reliable detection of mutated HBsAg. Currently, there is a striking shortage of data regarding the detection rates of in vivo HBsAg mutations for these clinically important assays. Therefore, we compared the detection rates of four commercial HBsAg screening assays using a global cohort of 1553 patients from four continents with known HBV genotypes. These samples, which represent the broadest spectrum of known and novel HBsAg major hydrophilic region (MHR) mutations to date, were analyzed for the presence of HBsAg using the Roche Elecsys® HBsAg II Qualitative, Siemens ADVIA Centaur XP HBsAg II, Abbott Architect HBsAg Qualitative II, and DiaSorin Liaison® HBsAg Qualitative assays, respectively...
April 16, 2018: Journal of Viral Hepatitis
Palak Shah, Baozhong Zhao, Lei Qiang, Yu-Ying He
Nucleotide excision repair (NER) is the most versatile DNA repair system that removes bulky DNA damage induced by various endogenous and exogenous factors, including UV radiation. Defects in NER can lead to the xeroderma pigmentosum (XP) syndrome, mainly characterized by increased carcinogenesis in the skin. The function of NER factors, including xeroderma pigmentosum group C (XPC), can be regulated by post-translational modifications such as ubiquitination. However, the role of phosphorylation in XPC function remains unknown...
April 6, 2018: Nucleic Acids Research
Jing Zhu, Lu Yan, Xiaoguang Xu, Yan Zhang, Junling Shi, Chunmei Jiang, Dongyan Shao
To improve the production yield of (+)-pinoresinol (Pin), (+)-pinoresinol monoglucoside (PMG), and (+)-pinoresinol diglucoside (PDG), different methods were conducted, including co-culture with resveratrol-producing Alternaria sp. MG1 spores and addition of Tu-chung in a medium at the start of cultivation, ultrasound treatment (40 kHZ, 10 min) on 5-day culture, and addition of ethanol and sodium butyrate on Day 3, followed by cultivation for an additional period of 2 days. At the end of the cultivation period (5 days), the liquid phase was collected for product analysis...
April 16, 2018: AMB Express
Maleeha Nayyer, Shahreen Zahid, Syed Hammad Hassan, Salman Aziz Mian, Sana Mehmood, Haroon Ahmed Khan, Muhammad Kaleem, Muhammad Sohail Zafar, Abdul Samad Khan
Objective: The objective of this study was to assess the surface properties (microhardness and wear resistance) of various composites and compomer materials. In addition, the methodologies used for assessing wear resistance were compared. Materials and Methods: This study was conducted using restorative material (Filtek Z250, Filtek Z350, QuiXfil, SureFil SDR, and Dyract XP) to assess wear resistance. A custom-made toothbrush simulator was employed for wear testing...
January 2018: European Journal of Dentistry
Xiaodan Chen, Zhongshu Li, Hansjörg Grützmacher
With respect to the well-developed chemistry of pyridines, that of phosphinines is in its infancy. There are still challenges to be overcome with respect to the development of simple syntheses and the application of phosphinine derivatives. Here, we show that sodium phosphinin-2-olate is a valuable building block to prepare multidentate phosphinine-ligands which in turn can be employed for the preparation of metal complexes. These Pd(II) and Rh(I) complexes were fully characterized, including NMR spectra and single X-ray diffraction studies, which clearly demonstrate that molecules R3-xP(O-phosphinine)x with one, two, and three phosphinines (x = 1, 2, 3) can be tethered to a central phosphorus donor center to give bis-, tris-, and tetradentate ligands...
April 12, 2018: Chemistry: a European Journal
Anne-Marie Hinds, Elisabeth Rosser, M Ashwin Reddy
The constellation of signs including microcephaly, retinal colobomas, and exudative vitreo-retinopathy suggests a mutation of the KIF-11 gene on chromosome 10q. We report a female infant with these features but due, instead, to a contiguous gene deletion on chromosome Xp including the OMIM morbid genes CASK, KDM6A, NDP, MAOA, NYX, and DDX3X. The NDP deletion could account for the exudative retinopathy and the CASK deletion for the microcephaly, while CASK and KDM6A have both been associated with coloboma. This case highlights genetic heterogeneity for the clustering of these signs...
April 4, 2018: Ophthalmic Genetics
Joonhong Park, Seungok Lee, Yeongsic Kim, Aeran Choi, Hyeyoung Lee, Jihyang Lim, Yonggoo Kim, Kyungja Han, Eun Jee Oh
BACKGROUND: Carcinoembryonic antigen (CEA) is one of the tumor markers available for evaluating disease progression status after initial therapy and monitoring subsequent treatment modalities in colorectal, gastrointestinal, lung, and breast carcinoma. We evaluated the correlations and differences between widely used, automated CEA immunoassays at four different medical laboratories. METHODS: In total, 393 serum samples with CEA ranging from 3.0 to 1,000 ng/mL were analyzed on ADVIA Centaur XP (Siemens Diagnostics, Tarrytown, NY, USA), ARCHITECT i2000sr (Abbott Diagnostics, Abbott Park, IL, USA), Elecsys E170 (Roche Diagnostics, Indianapolis, IN, USA), and Unicel DxI800 (Beckman Coulter, Fullerton, CA, USA), and the results were compared...
July 2018: Annals of Laboratory Medicine
Ioannis Papoulidis, Annalisa Vetro, Vassilis Paspaliaris, Monika Ziegler, Katharina Kreskowski, George Daskalakis, Vasilios Papadopoulos, Themistoklis Dagklis, Thomas Liehr, Loretta Thomaidis, Emmanouil Manolakos
Background: Short arm deletions of the X-chromosome are challenging issues for genetic counseling due to their low penetrance in population. Female carriers of these deletions have milder phenotype than male ones, considering the intellectual ability and social skills, probably because of the X-chromosome inactivation phenomenon. Case report: A female patient with a 10Mb distal Xp deletion and an Xq duplication, showing mild intellectual disability, is described in this report...
April 2018: Current Genomics
Francesco Maffessanti, Frits W Prinzen, Giulio Conte, François Regoli, Maria Luce Caputo, Daniel Suerder, Tiziano Moccetti, Francesco Faletra, Rolf Krause, Angelo Auricchio
OBJECTIVES: This study sought to test the accuracy of strain measurements based on anatomo-electromechanical mapping (AEMM) measurements compared with magnetic resonance imaging (MRI) tagging, to evaluate the diagnostic value of AEMM-based strain measurements in the assessment of myocardial viability, and the additional value of AEMM over peak-to-peak local voltages. BACKGROUND: The in vivo identification of viable tissue, evaluation of mechanical contraction, and simultaneous left ventricular activation is currently achieved using multiple complementary techniques...
January 2018: JACC. Clinical Electrophysiology
Ambreen Ijaz, Sulman Basit, Ajab Gul, Lilas Batool, Abrar Hussain, Sibtain Afzal, Khushnooda Ramzan, Jamil Ahmad, Abdul Wali
Xeroderma Pigmentosum (XP) is a rare autosomal recessive skin disorder characterized by hyperpigmentation, premature skin aging, ocular and cutaneous photosensitivity and increased risk of skin carcinoma. We investigated seven consanguineous XP families with nine patients from Pakistan. All the Patients exhibited typical clinical symptoms of XP since first year of life. Whole genome SNP genotyping identified a 14Mb autozygous region segregating with the disease phenotype on chromosome 3p25.1. DNA sequencing of XPC gene revealed a founder homozygous splice site mutation (c...
March 23, 2018: Congenital Anomalies
Huaixiang Zhou, Xusheng Cheng, Xiaoyuan Xu, Tianlong Jiang, Haimeng Zhou, Qing Sheng, Zuoming Nie
Alpha-tubulin N-acetyltransferase 1 (ATAT1) is an acetyltransferase specific to α-tubulin and performs important functions in many cellular processes. Bombyx mori is an economic insect and also known as a model lepidoptera insect. In this study, we cloned a B. mori ATAT1 gene (BmATAT1) (Gen Bank accession number: XP_004932777.1). BmATAT1 contained an open reading frame (ORF) of 1,065 bp encoding 355 amino acids (aa). Expression profiling of BmATAT1 protein showed that the expression levels of BmATAT1 at different developmental stages and different tissues in fifth-instar larvae differ...
March 22, 2018: Archives of Insect Biochemistry and Physiology
Gurkamaljit Kaur, Bharati Pandey, Arbind Kumar, Naina Garewal, Abhinav Grover, Jagdeep Kaur
The lipolytic protein LipU was conserved in mycobacterium sp. including M. tuberculosis (MTB LipU) and M. leprae (MLP LipU). The MTB LipU was identified in extracellular fraction and was reported to be essential for the survival of mycobacterium. Therefore to address the problem of drug resistance in pathogen, LipU was selected as a drug target and the viability of finding out some FDA approved drugs as LipU inhibitors in both the cases was explored. Three dimensional (3D) model structures of MTB LipU and MLP LipU were generated and stabilized through molecular dynamics (MD)...
March 20, 2018: Journal of Biomolecular Structure & Dynamics
Gülşah Uslu, Taha Özyürek, Koray Yılmaz, Mustafa Gündoğar, Gianluca Plotino
INTRODUCTION: The purpose of this study was to investigate the amount of apically extruded debris by Reciproc Blue (REC Blue; VDW, Munich, Germany), HyFlex EDM (HEDM; Coltene/Whaledent, Altstätten, Switzerland), and XP-endo Shaper (XPS; FKG Dentaire SA, La Chaux-de-Fonds, Switzerland) files during root canal preparation at body temperature. METHODS: Sixty extracted single-rooted mandibular premolar human teeth were randomly assigned to 3 groups (n = 20). The canals were instrumented using 1 of the following instruments: REC Blue, HEDM, or XPS...
March 14, 2018: Journal of Endodontics
Udo Bauer, Florian Späth, Fabian Düll, Philipp Bachmann, Johann Steinhauer, Hans-Peter Steinrück, Christian Papp
We have investigated the adsorption and thermal reactivity of CO and C2H4 on well-defined bimetallic PtxAg1-x/Pt(111) surface alloys (with x = 0.67, 0.50, 0.38 and 0.32) using in situ synchrotron-based high resolution X-ray photoelectron spectroscopy. During low temperature (140 K) adsorption of CO, we find population of the energetically more stable Pt on-top site followed by the Pt bridge site, as known from clean Pt(111); CO does not adsorb on Ag facets under the applied conditions. The total CO coverage linearly decreases with Ag surface content...
March 14, 2018: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
M Demirci, S Tuncer, H S Sancakli, N Tekçe, C Baydemir
The purpose of this study was to evaluate a nanofilled and a nanohybrid composite, in combination with manufacturer-recommended etch-and-rinse adhesives, in class IV cavities. Thirty-four patients aged 14-46 years (mean age, 27.1 years) comprised the study group. Twenty-six patients received two class IV restorations and eight patients received four class IV restorations. For each patient, half the number of restorations were performed using a nanohybrid composite (Ceram X duo) and the remaining half used a nanofilled resin composite (Filtek Supreme XT), with two- (XP Bond) and three-step (Scotchbond Multipurpose) etch-and-rinse adhesives, respectively...
March 13, 2018: Operative Dentistry
Yiqing Yang, Ruiqiong Guo, Kristen Gaffney, Miyeon Kim, Shaima Muhammednazaar, Wei Tian, Boshen Wang, Jie Liang, Heedeok Hong
ATP-dependent protein degradation mediated by AAA+ proteases is one of the major cellular pathways for protein quality control and regulation of functional networks. While a majority of studies of protein degradation have focused on water-soluble proteins, it is not well understood how membrane proteins with abnormal conformation are selectively degraded. The knowledge gap stems from the lack of an in vitro system in which detailed molecular mechanisms can be studied as well as difficulties in studying membrane protein folding in lipid bilayers...
April 4, 2018: Journal of the American Chemical Society
Ö I Ulusoy, I G Savur, T Alaçam, B Çelik
AIM: To evaluate the effectiveness of NaOCl, NaOCl-EDTA, and NaOCl+HEBP activated by ultrasonics and XP-endo Finisher on organic tissue removal from simulated internal root resorption cavities. METHODOLOGY: The root canals of 144 single-rooted teeth were instrumented. The teeth were split longitudinally and semicircular cavities were prepared in the canals on each half of the roots. Samples obtained from ground bovine muscle tissue were weighed and adapted into the semicircular cavities...
March 5, 2018: International Endodontic Journal
P Malaquias, M F Gutierrez, V Hass, R Stanislawczuk, M C Bandeca, Cag Arrais, P V Farago, A Reis, A D Loguercio
OBJECTIVES: To evaluate the effects of addition of diacetate chlorhexidine (CHX) at different concentrations into two etch-and-rinse adhesive systems on CHX release, as well as the immediate (IM) and two-year (2-Y) resin-dentin microtensile bond strength (μTBS) and nanoleakage (NL). METHODS: CHX was added to XP Bond (XP) and Ambar (AM) at concentrations of 0.0 wt% (control); 0.01 wt%; 0.05 wt%; and 0.1 to 0.2 wt%. To assess the cumulative CHX release, adhesive disks were made in a metallic matrix and after light-curing were stored in water...
March 2018: Operative Dentistry
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