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Collagen Fibrillogenesis

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https://www.readbyqxmd.com/read/27770943/insight-into-the-collagen-assembly-in-the-presence-of-lysine-and-glutamic-acid-an-in-vitro-study
#1
Xinhua Liu, Nianhua Dan, Weihua Dan
The aim of this study is to evaluate the effects of two different charged amino acids in collagen chains, lysine and glutamic acid, on the fibrillogenesis process of collagen molecules. The turbidity, zeta potential, and fiber diameter analysis suggest that introducing the positively charged lysine into collagen might improve the sizes or amounts of the self-assembled collagen fibrils significantly. Conversely, the negatively charged glutamic acid might restrict the self-assembly of collagen building blocks into a higher order structure...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27760365/nanoscale-imaging-of-collagen-gels-with-focused-ion-beam-milling-and-scanning-electron-microscopy
#2
Shawn P Reese, Niloofar Farhang, Randy Poulson, Gennie Parkman, Jeffrey A Weiss
In vitro polymerized type I collagen hydrogels have been used extensively as a model system for three-dimensional (3D) cell and tissue culture, studies of fibrillogenesis, and investigation of multiscale force transmission within connective tissues. The nanoscale organization of collagen fibrils plays an essential role in the mechanics of these gels and emergent cellular behavior in culture, yet quantifying 3D structure with nanoscale resolution to fully characterize fibril organization remains a significant technical challenge...
October 18, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27753891/ish-nia-os-06-putative-mechanisms-for-cerebrovascular-remodelling-in-the-spontaneously-hypertensive-rat-shr-using-transcriptomics
#3
Dawid Walas, Karol Nowicki-Osuch, Dominic Alibhai, Julian Paton
OBJECTIVE: Cerebrovascular remodeling in the SHR may be causative to the known brainstem hypoperfusion. Using RNA sequencing, we examined age-related processes that may govern remodeling of the cerebral arteries in the SHR. DESIGN AND METHOD: In SHR and their progenitor (normotensive) control (Wistar Kyoto, WKY), RNA-seq was performed at three ages: 5, 9, 13 weeks old. Cerebral arteries were flushed and peeled off the brain, stripped of meninges, snap frozen and RNA extracted...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27693454/decorin-interacting-network-a-comprehensive-analysis-of-decorin-binding-partners-and-their-versatile-functions
#4
Maria A Gubbiotti, Sylvain D Vallet, Sylvie Ricard-Blum, Renato V Iozzo
Decorin, a prototype small leucine-rich proteoglycan, regulates a vast array of cellular processes including collagen fibrillogenesis, wound repair, angiostasis, tumor growth, and autophagy. This functional versatility arises from a wide array of decorin/protein interactions also including interactions with its single glycosaminoglycan side chain. The decorin-binding partners encompass numerous categories ranging from extracellular matrix molecules to cell surface receptors to growth factors and enzymes. Despite the diversity of the decorin interacting network, two main roles emerge as prominent themes in decorin function: maintenance of cellular structure and outside-in signaling, culminating in anti-tumorigenic effects...
September 2016: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/27643237/ish-nia-os-06-putative-mechanisms-for-cerebrovascular-remodelling-in-the-spontaneously-hypertensive-rat-shr-using-transcriptomics
#5
Dawid Walas, Karol Nowicki-Osuch, Dominic Alibhai, Julian Paton
OBJECTIVE: Cerebrovascular remodeling in the SHR may be causative to the known brainstem hypoperfusion. Using RNA sequencing, we examined age-related processes that may govern remodeling of the cerebral arteries in the SHR. DESIGN AND METHOD: In SHR and their progenitor (normotensive) control (Wistar Kyoto, WKY), RNA-seq was performed at three ages: 5, 9, 13 weeks old. Cerebral arteries were flushed and peeled off the brain, stripped of meninges, snap frozen and RNA extracted...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27586416/pleiotropic-roles-of-the-matricellular-protein-sparc-in-tendon-maturation-and-ageing
#6
Renate Gehwolf, Andrea Wagner, Christine Lehner, Amy D Bradshaw, Cornelia Scharler, Justyna A Niestrawska, Gerhard A Holzapfel, Hans-Christian Bauer, Herbert Tempfer, Andreas Traweger
Acute and chronic tendinopathies remain clinically challenging and tendons are predisposed to degeneration or injury with age. Despite the high prevalence of tendon disease in the elderly, our current understanding of the mechanisms underlying the age-dependent deterioration of tendon function remains very limited. Here, we show that Secreted protein acidic and rich in cysteine (Sparc) expression significantly decreases in healthy-aged mouse Achilles tendons. Loss of Sparc results in tendon collagen fibrillogenesis defects and Sparc-/- tendons are less able to withstand force in comparison with their respective wild type counterparts...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27519293/detection-of-type-i-collagen-fibrils-formation-and-dissociation-by-a-fluorescence-method-based-on-thioflavin-t
#7
Meilian Zou, Huan Yang, Haibo Wang, Haiyin Wang, Juntao Zhang, Benmei Wei, Hanjun Zhang, Dong Xie
In this study, fibrillogenesis and thermal dissociation of pepsin-soluble collagen (PSC), extracted from snakehead (Channa argus) skin, were monitored by fluorescence method based on thioflavin T (Th-T), where the accuracy and sensitivity were evaluated and compared with those of turbidity assay. The fluorescence method revealed the fibrillogenesis dynamics of collagen with better sensitivity, especially at nucleation and plateau stages. The melting temperature (Tm) of PSC was estimated to be 47°C by circular dichroism spectroscopy; below this temperature, the triple-helical structure should be intact...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27484779/control-of-fibrotic-changes-through-the-synergistic-effects-of-anti-fibronectin-antibody-and-an-rgds-tagged-form-of-the-same-antibody
#8
Anil Tiwari, Rajendra Kumar, Jagat Ram, Maryada Sharma, Manni Luthra-Guptasarma
TGF-β and myofibroblasts play a key role in fibrosis, characterized by aberrant synthesis and deposition of extracellular matrix (ECM) proteins, such as fibronectin (Fn) and collagen type I. There are two major roles played by integrins in the fibrotic pathology: (i) Fn-integrin interaction, coupled with cytokines like TGF-β, facilitates the self-polymerization of Fn and regulates cell-matrix fibrillar adhesions, thereby promoting fibrillogenesis; (ii) Integrin interaction with an RGD (arginine-glycine-aspartic) consensus sequence in the latent TGF-β, resulting in its activation...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27375477/tendon-extracellular-matrix-alterations-in-ullrich-congenital-muscular-dystrophy
#9
Francesca Sardone, Francesco Traina, Alice Bondi, Luciano Merlini, Spartaco Santi, Nadir Mario Maraldi, Cesare Faldini, Patrizia Sabatelli
Collagen VI (COLVI) is a non-fibrillar collagen expressed in skeletal muscle and most connective tissues. Mutations in COLVI genes cause two major clinical forms, Bethlem myopathy and Ullrich congenital muscular dystrophy (UCMD). In addition to congenital muscle weakness, patients affected by COLVI myopathies show axial and proximal joint contractures and distal joint hypermobility, which suggest the involvement of the tendon function. We examined a peroneal tendon biopsy and tenocyte culture of a 15-year-old patient affected by UCMD with compound heterozygous COL6A2 mutations...
2016: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/27234502/periostin-as-a-multifunctional-modulator-of-the-wound-healing-response
#10
REVIEW
John T Walker, Karrington McLeod, Shawna Kim, Simon J Conway, Douglas W Hamilton
During tissue healing, the dynamic and temporal alterations required for effective repair occur in the structure and composition of the extracellular matrix (ECM). Matricellular proteins (MPs) are a group of diverse non-structural ECM components that bind cell surface receptors mediating interactions between the cell and its microenviroment, effectively regulating adhesion, migration, proliferation, signaling, and cell phenotype. Periostin (Postn), a pro-fibrogenic secreted glycoprotein, is defined as an MP based on its expression pattern and regulatory roles during development and healing and in disease processes...
September 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27162200/the-collagen-type-i-segment-long-spacing-sls-and-fibrillar-forms-formation-by-atp-and-sulphonated-diazo-dyes
#11
J Robin Harris, Richard J Lewis
The collagen type I segment long spacing (SLS) crystallite is a well-ordered rod-like molecular aggregate, ∼300nm in length, which is produced in vitro under mildly acidic conditions (pH 2.5-3.5) in the presence of 1mM ATP. The formation of the SLS crystallite amplifies the inherent linear structural features of individual collagen heterotrimers, due to the punctate linear distribution and summation of the bulkier amino acid side chains along the length of individual collagen heterotrimers. This can be correlated structurally with the 67nm D-banded collagen fibril that is found in vivo, and formed in vitro...
July 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27115146/decellularized-aortic-conduits-could-their-cryopreservation-affect-post-implantation-outcomes-a-morpho-functional-study-on-porcine-homografts
#12
Michele Gallo, Antonella Bonetti, Helen Poser, Filippo Naso, Tomaso Bottio, Roberto Bianco, Adolfo Paolin, Paolo Franci, Roberto Busetto, Anna Chiara Frigo, Edward Buratto, Michele Spina, Maurizio Marchini, Fulvia Ortolani, Laura Iop, Gino Gerosa
Decellularized porcine aortic valve conduits (AVCs) implanted in a Vietnamese Pig (VP) experimental animal model were matched against decellularized and then cryopreserved AVCs to assess the effect of cryopreservation on graft hemodynamic performance and propensity to in vivo repopulation by host's cells. VPs (n = 12) underwent right ventricular outflow tract substitution using AVC allografts and were studied for 15-month follow-up. VPs were randomized into two groups, receiving AVCs treated with decellularization alone (D; n = 6) or decellularization/cryopreservation (DC; n = 6), respectively...
April 26, 2016: Heart and Vessels
https://www.readbyqxmd.com/read/26899371/high-throughput-proteomics-reveal-alarmins-as-amplifiers-of-tissue-pathology-and-inflammation-after-spinal-cord-injury
#13
Athanasios Didangelos, Michele Puglia, Michaela Iberl, Candela Sanchez-Bellot, Bernd Roschitzki, Elizabeth J Bradbury
Spinal cord injury is characterized by acute cellular and axonal damage followed by aggressive inflammation and pathological tissue remodelling. The biological mediators underlying these processes are still largely unknown. Here we apply an innovative proteomics approach targeting the enriched extracellular proteome after spinal cord injury for the first time. Proteomics revealed multiple matrix proteins not previously associated with injured spinal tissue, including small proteoglycans involved in cell-matrix adhesion and collagen fibrillogenesis...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26818589/myofibroblast-secretome-and-its-auto-paracrine-signaling
#14
Ritin Bomb, Mark R Heckle, Yao Sun, Salvatore Mancarella, Ramareddy V Guntaka, Ivan C Gerling, Karl T Weber
Myofibroblasts (myoFb) are phenotypically transformed, contractile fibroblast-like cells expressing α-smooth muscle actin microfilaments. They are integral to collagen fibrillogenesis with scar tissue formation at sites of repair irrespective of the etiologic origins of injury or tissue involved. MyoFb can persist long after healing is complete, where their ongoing turnover of collagen accounts for a progressive structural remodeling of an organ (a.k.a. fibrosis, sclerosis or cirrhosis). Such persistent metabolic activity is derived from a secretome consisting of requisite components in the de novo generation of angiotensin (Ang) II...
2016: Expert Review of Cardiovascular Therapy
https://www.readbyqxmd.com/read/26711913/forelimb-contractures-and-abnormal-tendon-collagen-fibrillogenesis-in-fibulin-4-null-mice
#15
Dessislava Z Markova, Te-Cheng Pan, Rui-Zhu Zhang, Guiyun Zhang, Takako Sasaki, Machiko Arita, David E Birk, Mon-Li Chu
Fibulin-4 is an extracellular matrix glycoprotein essential for elastic fiber formation. Mice deficient in fibulin-4 die perinatally because of severe pulmonary and vascular defects associated with the lack of intact elastic fibers. Patients with fibulin-4 mutations demonstrate similar defects, and a significant number die shortly after birth or in early childhood from cardiopulmonary failure. The patients also demonstrate skeletal and other systemic connective tissue abnormalities, including joint laxity and flexion contractures of the wrist...
June 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/26600400/the-small-leucine-rich-proteoglycan-fibromodulin-is-overexpressed-in-human-prostate-epithelial-cancer-cell-lines-in-culture-and-human-prostate-cancer-tissue
#16
Niradiz Reyes, Ines Benedetti, Alfonso Bettin, Juan Rebollo, Jan Geliebter
BACKGROUND: Fibromodulin is a small leucine-rich proteoglycan important for extracellular matrix organization and essential for tissue repair in multiple organs. The main function of this proteoglycan is the regulation of collagen fibrillogenesis; however, more recently described roles for fibromodulin have expanded to include regulation of angiogenesis, reprogramming of human fibroblasts into pluripotent cells, modulation of TGF-β activity, inflammatory processes and association with metastatic phenotypes...
2016: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/26562557/feasibility-study-of-the-natural-derived-chitosan-dialdehyde-for-chemical-modification-of-collagen
#17
Xinhua Liu, Nianhua Dan, Weihua Dan, Juxia Gong
The aim of this study is to evaluate the chemical crosslinking effects of the natural derived chitosan dialdehyde (OCS) on collagen. Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC) and circular dichroism (CD) measurements suggest that introducing OCS might not destroy the natural triple helix conformation of collagen but enhance the thermal-stability of collagen. Meanwhile, a denser fibrous network of cross-linked collagen is observed by atomic force microscopy. Further, scanning electron microscopy (SEM) and aggregation kinetics analysis confirm that the fibrillation process of collagen advances successfully and OCS could lengthen the completion time of collagen fibrillogenesis but raise the reconstitution rate of collagen fibrils or microfibrils...
January 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/26554635/restraint-of-trophoblast-invasion-of-the-uterus-by-decorin-role-in-pre-eclampsia
#18
REVIEW
Pinki Nandi, Mohammad Fyyaz Siddiqui, Peeyush K Lala
Decorin (DCN) is a leucine-rich, TGF-β binding proteoglycan produced by mesenchymal cells including chondrocytes, dermal fibroblasts, and uterine decidual cells. It exerts multiple physiological functions including collagen fibrillogenesis, myogenesis, angiostasis, and restraining placental invasiveness. We discovered that decidua-derived DCN restrains proliferation, migration, and invasion of extravillous trophoblast (EVT) cells of the human placenta in a TGF-β-independent manner. These functions were differentially mediated by binding of DCN to multiple tyrosine kinase receptors (TKR) including EGFR, IGFR1, and VEGFR2...
March 2016: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/26488368/development-and-characterization-of-a-rapid-polymerizing-collagen-for-soft-tissue-augmentation
#19
D DeVore, J Zhu, R Brooks, R Rone-McCrate, D A Grant, S A Grant
A liquid collagen has been developed that fibrilizes upon injection. Rapid polymerizing collagen (RPC) is a type I porcine collagen that undergoes fibrillization upon interaction with ionic solutions, such as physiological solutions. The ability to inject liquid collagen would be beneficial for many soft tissue augmentation applications. In this study, RPC was synthesized and characterized as a possible dermal filler. Transmission electron microscopy, ion induced RPC fibrillogenesis tests, collagenase resistance assay, and injection force studies were performed to assess RPC's physicochemical properties...
October 21, 2015: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/26452150/bioinspired-collagen-glycosaminoglycan-based-cellular-microenvironments-for-tuning-osteoclastogenesis
#20
Sandra Rother, Juliane Salbach-Hirsch, Stephanie Moeller, Thomas Seemann, Matthias Schnabelrauch, Lorenz C Hofbauer, Vera Hintze, Dieter Scharnweber
Replicating the biocomplexity of native extracellular matrices (ECM) is critical for a deeper understanding of biochemical signals influencing bone homeostasis. This will foster the development of bioinspired biomaterials with adjustable bone-inducing properties. Collagen-based coatings containing single HA derivatives have previously been reported to promote osteogenic differentiation and modulate osteoclastogenesis and resorption depending on their sulfation degree. However, the potential impact of different GAG concentrations as well as the interplay of multiple GAGs in these coatings is not characterized in detail to date...
October 28, 2015: ACS Applied Materials & Interfaces
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