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Collagen Fibrillogenesis

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https://www.readbyqxmd.com/read/28216298/cell-instructive-starpeg-heparin-collagen-composite-matrices
#1
Marcus Binner, Laura J Bray, Jens Friedrichs, Uwe Freudenberg, Mikhail V Tsurkan, Carsten Werner
: Polymer hydrogels can be readily modulated with regard to their physical properties and functionalized to recapitulate molecular cues of the extracellular matrix (ECM). However, they remain structurally different from the hierarchical supramolecular assemblies of natural ECM. Accordingly, we herein report a set of hydrogel composite materials made from starPEG-peptide conjugates, maleimide-functionalized heparin and collagen type I that combine semisynthetic and ECM-derived components...
February 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28192200/the-binding-capacity-of-%C3%AE-1%C3%AE-1-%C3%AE-2%C3%AE-1-and-%C3%AE-10%C3%AE-1-integrins-depends-on-non-collagenous-surface-macromolecules-rather-than-the-collagens-in-cartilage-fibrils
#2
Christian Woltersdorf, Melanie Bonk, Birgit Leitinger, Mikko Huhtala, Jarmo Käpylä, Jyrki Heino, Christian Gil Girol, Stephan Niland, Johannes A Eble, Peter Bruckner, Rita Dreier, Uwe Hansen
Interactions of cells with supramolecular aggregates of the extracellular matrix (ECM) are mediated, in part, by cell surface receptors of the integrin family. These are important molecular components of cell surface-suprastructures regulating cellular activities in general. A subfamily of β1-integrins with von Willebrand-factor A-like domains (I-domains) in their α-chains can bind to collagen molecules and, therefore, are considered as important cellular mechano-receptors. Here we show that chondrocytes strongly bind to cartilage collagens in the form of individual triple helical molecules but very weakly to fibrils formed by the same molecules...
February 10, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28181591/lumican-peptides-rational-design-targeting-alk5-tgfbri
#3
Tarsis Ferreira Gesteira, Vivien J Coulson-Thomas, Yong Yuan, Jianhua Zhang, Helena B Nader, Winston W-Y Kao
Lumican, a small leucine rich proteoglycan (SLRP), is a component of extracellular matrix which also functions as a matrikine regulating multiple cell activities. In the cornea, lumican maintains corneal transparency by regulating collagen fibrillogenesis, promoting corneal epithelial wound healing, regulating gene expression and maintaining corneal homeostasis. We have recently shown that a peptide designed from the 13 C-terminal amino acids of lumican (LumC13) binds to ALK5/TGFBR1 (type1 receptor of TGFβ) to promote wound healing...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28146148/anisotropically-organized-three-dimensional-culture-platform-for-reconstruction-of-a-hippocampal-neural-network
#4
So Hyun Kim, Sun-Kyoung Im, Soo-Jin Oh, Sohyeon Jeong, Eui-Sung Yoon, C Justin Lee, Nakwon Choi, Eun-Mi Hur
In native tissues, cellular and acellular components are anisotropically organized and often aligned in specific directions, providing structural and mechanical properties for actuating biological functions. Thus, engineering alignment not only allows for emulation of native tissue structures but might also enable implementation of specific functionalities. However, achieving desired alignment is challenging, especially in three-dimensional constructs. By exploiting the elastomeric property of polydimethylsiloxane and fibrillogenesis kinetics of collagen, here we introduce a simple yet effective method to assemble and align fibrous structures in a multi-modular three-dimensional conglomerate...
February 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/28127105/fibromodulin-expression-in-folliculostellate-cells-and-pericytes-is-promoted-by-tgf%C3%AE-signaling-in-rat-anterior-pituitary-gland
#5
Rahimi Syaidah, Takehiro Tsukada, Morio Azuma, Kotaro Horiguchi, Ken Fujiwara, Motoshi Kikuchi, Takashi Yashiro
Fibromodulin belongs to the family of small leucine-rich proteoglycans (SLRPs), an active component of extracellular matrix. It directly binds collagens to promote fibrillogenesis and also binds transforming growth factor-beta (TGFβ) to antagonize its actions. Our previous studies of rat anterior pituitary gland revealed that fibromodulin is expressed in folliculostellate cells and pericytes. Although our recent study showed that TGFβ2 secreted from folliculostellate cells induces collagen synthesis in pericytes, the involvement of fibromodulin in TGFβ2-mediated collagen regulation has not been studied...
December 28, 2016: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28102596/delineation-of-ehlers-danlos-syndrome-phenotype-due-to-the-c-934c-t-p-arg312cys-mutation-in-col1a1-report-on-a-three-generation-family-without-cardiovascular-events-and-literature-review
#6
Marina Colombi, Chiara Dordoni, Marina Venturini, Arianna Zanca, Piergiacomo Calzavara-Pinton, Marco Ritelli
Classical Ehlers-Danlos syndrome (cEDS) is a rare connective tissue disorder primarily characterized by hyperextensible skin, defective wound healing, abnormal scars, easy bruising, and generalized joint hypermobility; arterial dissections are rarely observed. Mutations in COL5A1 and COL5A2 encoding type V collagen account for more than 90% of the patients so far characterized. In addition, cEDS phenotype was reported in a small number of patients carrying the c.934C>T mutation in COL1A1 that results in an uncommon substitution of a non-glycine residue in one Gly-Xaa-Yaa repeat of the pro-α1(I)-chain p...
February 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28042273/gingival-crevicular-fluid-periostin-levels-in-chronic-periodontitis-patients-following-nonsurgical-periodontal-treatment-with-low-level-laser-therapy
#7
Dhanangchaayan Kumaresan, Aruna Balasundaram, Vanaja Krishna Naik, Deva Priya Appukuttan
OBJECTIVE: Periostin is a matricellular protein highly expressed in periosteum, periodontal ligament and is essential for tissue integrity and maturation. It plays a role in collagen fibrillogenesis and is downregulated in periodontal disease. Biostimulation utilizing low-level laser therapy (LLLT) influences periodontal ligament fibroblast proliferation. This study was conducted with the objective of estimating periostin levels in chronic periodontitis (CP) patients following LLLT as an adjunct to root surface debridement (RSD)...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28017821/identification-of-a-p-arg708gln-variant-in-col1a2-in-atypical-femoral-fractures
#8
Thomas Funck-Brentano, Agnes Ostertag, Francoise Debiais, Patrice Fardellone, Corinne Collet, Etienne Mornet, Martine Cohen-Solal
OBJECTIVES: Long-term bisphosphonates exposure is a proven risk factor for atypical femoral fractures (AFF) but several cases occur in untreated patients. The identification of other risk factors for AFF is critical for the management of osteoporosis. We here assessed the genetic factors associated with AFF regardless of the treatment. METHODS: Cases were identified through ICD-10 codes in 3 academic centers. Medical records were analyzed by 2 investigators that adjudicated X-rays for typical or atypical fractures...
December 23, 2016: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/28011632/a-developmental-transcriptomic-analysis-of-pax1-and-pax9-in-embryonic-intervertebral-disc-development
#9
V Sivakamasundari, Petra Kraus, Wenjie Sun, Xiaoming Hu, Siew Lan Lim, Shyam Prabhakar, Thomas Lufkin
Pax1 and Pax9 play redundant, synergistic functions in the patterning and differentiation of the sclerotomal cells that give rise to the vertebral bodies and intervertebral discs (IVD) of the axial skeleton. They are conserved in mice and humans, whereby mutation/deficiency of human PAX1/PAX9 has been associated with kyphoscoliosis. By combining cell-type-specific transcriptome and ChIP-sequencing data, we identified the roles of Pax1/Pax9 in cell proliferation, cartilage development and collagen fibrillogenesis, which are vital in early IVD morphogenesis...
February 15, 2017: Biology Open
https://www.readbyqxmd.com/read/27928505/collagen-a-network-for-regenerative-medicine
#10
REVIEW
K M Pawelec, S M Best, R E Cameron
The basic building block of the extra-cellular matrix in native tissue is collagen. As a structural protein, collagen has an inherent biocompatibility making it an ideal material for regenerative medicine. Cellular response, mediated by integrins, is dictated by the structure and chemistry of the collagen fibers. Fiber formation, via fibrillogenesis, can be controlled in vitro by several factors: pH, ionic strength, and collagen structure. After formation, fibers are stabilized via cross-linking. The final bioactivity of collagen scaffolds is a result of both processes...
October 28, 2016: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/27926839/circular-dichroism-spectroscopy-of-collagen-fibrillogenesis-a-new-use-for-an-old-technique
#11
Kathryn E Drzewiecki, Daniel R Grisham, Avanish S Parmar, Vikas Nanda, David I Shreiber
Type-I collagen assembles in a stepwise, hierarchic fashion from the folding of the triple helix to the assembly of fibrils into fibers. The mature assembled fibers are crucial for tissue structure and mechanics, cell interactions, and other functions in vivo. Although triple helix folding can be followed with the use of optical methods such as circular dichroism (CD) spectroscopy, fibrillogenesis is typically measured by alternative methods such as turbidity, rheology, and microscopy. Together, these approaches allow for investigation of the mechanical properties and architectures of collagen-based scaffolds and excised tissues...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27770943/insight-into-the-collagen-assembly-in-the-presence-of-lysine-and-glutamic-acid-an-in-vitro-study
#12
Xinhua Liu, Nianhua Dan, Weihua Dan
The aim of this study is to evaluate the effects of two different charged amino acids in collagen chains, lysine and glutamic acid, on the fibrillogenesis process of collagen molecules. The turbidity, zeta potential, and fiber diameter analysis suggest that introducing the positively charged lysine into collagen might improve the sizes or amounts of the self-assembled collagen fibrils significantly. Conversely, the negatively charged glutamic acid might restrict the self-assembly of collagen building blocks into a higher order structure...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27760365/nanoscale-imaging-of-collagen-gels-with-focused-ion-beam-milling-and-scanning-electron-microscopy
#13
Shawn P Reese, Niloofar Farhang, Randy Poulson, Gennie Parkman, Jeffrey A Weiss
In vitro polymerized type I collagen hydrogels have been used extensively as a model system for three-dimensional (3D) cell and tissue culture, studies of fibrillogenesis, and investigation of multiscale force transmission within connective tissues. The nanoscale organization of collagen fibrils plays an essential role in the mechanics of these gels and emergent cellular behavior in culture, yet quantifying 3D structure with nanoscale resolution to fully characterize fibril organization remains a significant technical challenge...
October 18, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27753891/ish-nia-os-06-putative-mechanisms-for-cerebrovascular-remodelling-in-the-spontaneously-hypertensive-rat-shr-using-transcriptomics
#14
Dawid Walas, Karol Nowicki-Osuch, Dominic Alibhai, Julian Paton
OBJECTIVE: Cerebrovascular remodeling in the SHR may be causative to the known brainstem hypoperfusion. Using RNA sequencing, we examined age-related processes that may govern remodeling of the cerebral arteries in the SHR. DESIGN AND METHOD: In SHR and their progenitor (normotensive) control (Wistar Kyoto, WKY), RNA-seq was performed at three ages: 5, 9, 13 weeks old. Cerebral arteries were flushed and peeled off the brain, stripped of meninges, snap frozen and RNA extracted...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27693454/decorin-interacting-network-a-comprehensive-analysis-of-decorin-binding-partners-and-their-versatile-functions
#15
Maria A Gubbiotti, Sylvain D Vallet, Sylvie Ricard-Blum, Renato V Iozzo
Decorin, a prototype small leucine-rich proteoglycan, regulates a vast array of cellular processes including collagen fibrillogenesis, wound repair, angiostasis, tumor growth, and autophagy. This functional versatility arises from a wide array of decorin/protein interactions also including interactions with its single glycosaminoglycan side chain. The decorin-binding partners encompass numerous categories ranging from extracellular matrix molecules to cell surface receptors to growth factors and enzymes. Despite the diversity of the decorin interacting network, two main roles emerge as prominent themes in decorin function: maintenance of cellular structure and outside-in signaling, culminating in anti-tumorigenic effects...
September 2016: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/27643237/ish-nia-os-06-putative-mechanisms-for-cerebrovascular-remodelling-in-the-spontaneously-hypertensive-rat-shr-using-transcriptomics
#16
Dawid Walas, Karol Nowicki-Osuch, Dominic Alibhai, Julian Paton
OBJECTIVE: Cerebrovascular remodeling in the SHR may be causative to the known brainstem hypoperfusion. Using RNA sequencing, we examined age-related processes that may govern remodeling of the cerebral arteries in the SHR. DESIGN AND METHOD: In SHR and their progenitor (normotensive) control (Wistar Kyoto, WKY), RNA-seq was performed at three ages: 5, 9, 13 weeks old. Cerebral arteries were flushed and peeled off the brain, stripped of meninges, snap frozen and RNA extracted...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27586416/pleiotropic-roles-of-the-matricellular-protein-sparc-in-tendon-maturation-and-ageing
#17
Renate Gehwolf, Andrea Wagner, Christine Lehner, Amy D Bradshaw, Cornelia Scharler, Justyna A Niestrawska, Gerhard A Holzapfel, Hans-Christian Bauer, Herbert Tempfer, Andreas Traweger
Acute and chronic tendinopathies remain clinically challenging and tendons are predisposed to degeneration or injury with age. Despite the high prevalence of tendon disease in the elderly, our current understanding of the mechanisms underlying the age-dependent deterioration of tendon function remains very limited. Here, we show that Secreted protein acidic and rich in cysteine (Sparc) expression significantly decreases in healthy-aged mouse Achilles tendons. Loss of Sparc results in tendon collagen fibrillogenesis defects and Sparc-/- tendons are less able to withstand force in comparison with their respective wild type counterparts...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27519293/detection-of-type-i-collagen-fibrils-formation-and-dissociation-by-a-fluorescence-method-based-on-thioflavin-t
#18
Meilian Zou, Huan Yang, Haibo Wang, Haiyin Wang, Juntao Zhang, Benmei Wei, Hanjun Zhang, Dong Xie
In this study, fibrillogenesis and thermal dissociation of pepsin-soluble collagen (PSC), extracted from snakehead (Channa argus) skin, were monitored by fluorescence method based on thioflavin T (Th-T), where the accuracy and sensitivity were evaluated and compared with those of turbidity assay. The fluorescence method revealed the fibrillogenesis dynamics of collagen with better sensitivity, especially at nucleation and plateau stages. The melting temperature (Tm) of PSC was estimated to be 47°C by circular dichroism spectroscopy; below this temperature, the triple-helical structure should be intact...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27484779/control-of-fibrotic-changes-through-the-synergistic-effects-of-anti-fibronectin-antibody-and-an-rgds-tagged-form-of-the-same-antibody
#19
Anil Tiwari, Rajendra Kumar, Jagat Ram, Maryada Sharma, Manni Luthra-Guptasarma
TGF-β and myofibroblasts play a key role in fibrosis, characterized by aberrant synthesis and deposition of extracellular matrix (ECM) proteins, such as fibronectin (Fn) and collagen type I. There are two major roles played by integrins in the fibrotic pathology: (i) Fn-integrin interaction, coupled with cytokines like TGF-β, facilitates the self-polymerization of Fn and regulates cell-matrix fibrillar adhesions, thereby promoting fibrillogenesis; (ii) Integrin interaction with an RGD (arginine-glycine-aspartic) consensus sequence in the latent TGF-β, resulting in its activation...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27375477/tendon-extracellular-matrix-alterations-in-ullrich-congenital-muscular-dystrophy
#20
Francesca Sardone, Francesco Traina, Alice Bondi, Luciano Merlini, Spartaco Santi, Nadir Mario Maraldi, Cesare Faldini, Patrizia Sabatelli
Collagen VI (COLVI) is a non-fibrillar collagen expressed in skeletal muscle and most connective tissues. Mutations in COLVI genes cause two major clinical forms, Bethlem myopathy and Ullrich congenital muscular dystrophy (UCMD). In addition to congenital muscle weakness, patients affected by COLVI myopathies show axial and proximal joint contractures and distal joint hypermobility, which suggest the involvement of the tendon function. We examined a peroneal tendon biopsy and tenocyte culture of a 15-year-old patient affected by UCMD with compound heterozygous COL6A2 mutations...
2016: Frontiers in Aging Neuroscience
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