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Collagen Fibrillogenesis

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https://www.readbyqxmd.com/read/28042273/gingival-crevicular-fluid-periostin-levels-in-chronic-periodontitis-patients-following-nonsurgical-periodontal-treatment-with-low-level-laser-therapy
#1
Dhanangchaayan Kumaresan, Aruna Balasundaram, Vanaja Krishna Naik, Deva Priya Appukuttan
OBJECTIVE: Periostin is a matricellular protein highly expressed in periosteum, periodontal ligament and is essential for tissue integrity and maturation. It plays a role in collagen fibrillogenesis and is downregulated in periodontal disease. Biostimulation utilizing low-level laser therapy (LLLT) influences periodontal ligament fibroblast proliferation. This study was conducted with the objective of estimating periostin levels in chronic periodontitis (CP) patients following LLLT as an adjunct to root surface debridement (RSD)...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28017821/identification-of-a-p-arg708gln-variant-in-col1a2-in-atypical-femoral-fractures
#2
Funck-Brentano Thomas, Ostertag Agnes, Debiais Francoise, Fardellone Patrice, Corinne Collet, Mornet Etienne, Cohen-Solal Martine
OBJECTIVES: Long-term bisphosphonates exposure is a proven risk factor for atypical femoral fractures (AFF) but several cases occur in untreated patients. The identification of other risk factors for AFF is critical for the management of osteoporosis. We here assessed the genetic factors associated with AFF regardless of the treatment. METHODS: Cases were identified through ICD-10 codes in 3 academic centers. Medical records were analyzed by 2 investigators that adjudicated X-rays for typical or atypical fractures...
December 22, 2016: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/28011632/a-developmental-transcriptomic-analysis-of-pax1-and-pax9-in-embryonic-intervertebral-disc-development
#3
V Sivakamasundari, Petra Kraus, Wenjie Sun, Xiaoming Hu, Siew Lan Lim, Shyam Prabhakar, Thomas Lufkin
Pax1 and Pax9 play redundant, synergistic functions in the patterning and differentiation of the sclerotomal cells that give rise to the vertebral bodies and intervertebral discs (IVD) of the axial skeleton. They are conserved in mice and humans, whereby mutations/deficiency of human PAX1/PAX9 have been associated with kyphoscoliosis. By combining cell-type specific transcriptome and ChIP-sequencing data, we identified the roles of Pax1/Pax9 in cell proliferation, cartilage development and collagen fibrillogenesis, which are vital in early IVD morphogenesis...
December 23, 2016: Biology Open
https://www.readbyqxmd.com/read/27928505/collagen-a-network-for-regenerative-medicine
#4
REVIEW
K M Pawelec, S M Best, R E Cameron
The basic building block of the extra-cellular matrix in native tissue is collagen. As a structural protein, collagen has an inherent biocompatibility making it an ideal material for regenerative medicine. Cellular response, mediated by integrins, is dictated by the structure and chemistry of the collagen fibers. Fiber formation, via fibrillogenesis, can be controlled in vitro by several factors: pH, ionic strength, and collagen structure. After formation, fibers are stabilized via cross-linking. The final bioactivity of collagen scaffolds is a result of both processes...
October 28, 2016: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/27926839/circular-dichroism-spectroscopy-of-collagen-fibrillogenesis-a-new-use-for-an-old-technique
#5
Kathryn E Drzewiecki, Daniel R Grisham, Avanish S Parmar, Vikas Nanda, David I Shreiber
Type-I collagen assembles in a stepwise, hierarchic fashion from the folding of the triple helix to the assembly of fibrils into fibers. The mature assembled fibers are crucial for tissue structure and mechanics, cell interactions, and other functions in vivo. Although triple helix folding can be followed with the use of optical methods such as circular dichroism (CD) spectroscopy, fibrillogenesis is typically measured by alternative methods such as turbidity, rheology, and microscopy. Together, these approaches allow for investigation of the mechanical properties and architectures of collagen-based scaffolds and excised tissues...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27770943/insight-into-the-collagen-assembly-in-the-presence-of-lysine-and-glutamic-acid-an-in-vitro-study
#6
Xinhua Liu, Nianhua Dan, Weihua Dan
The aim of this study is to evaluate the effects of two different charged amino acids in collagen chains, lysine and glutamic acid, on the fibrillogenesis process of collagen molecules. The turbidity, zeta potential, and fiber diameter analysis suggest that introducing the positively charged lysine into collagen might improve the sizes or amounts of the self-assembled collagen fibrils significantly. Conversely, the negatively charged glutamic acid might restrict the self-assembly of collagen building blocks into a higher order structure...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27760365/nanoscale-imaging-of-collagen-gels-with-focused-ion-beam-milling-and-scanning-electron-microscopy
#7
Shawn P Reese, Niloofar Farhang, Randy Poulson, Gennie Parkman, Jeffrey A Weiss
In vitro polymerized type I collagen hydrogels have been used extensively as a model system for three-dimensional (3D) cell and tissue culture, studies of fibrillogenesis, and investigation of multiscale force transmission within connective tissues. The nanoscale organization of collagen fibrils plays an essential role in the mechanics of these gels and emergent cellular behavior in culture, yet quantifying 3D structure with nanoscale resolution to fully characterize fibril organization remains a significant technical challenge...
October 18, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27753891/ish-nia-os-06-putative-mechanisms-for-cerebrovascular-remodelling-in-the-spontaneously-hypertensive-rat-shr-using-transcriptomics
#8
Dawid Walas, Karol Nowicki-Osuch, Dominic Alibhai, Julian Paton
OBJECTIVE: Cerebrovascular remodeling in the SHR may be causative to the known brainstem hypoperfusion. Using RNA sequencing, we examined age-related processes that may govern remodeling of the cerebral arteries in the SHR. DESIGN AND METHOD: In SHR and their progenitor (normotensive) control (Wistar Kyoto, WKY), RNA-seq was performed at three ages: 5, 9, 13 weeks old. Cerebral arteries were flushed and peeled off the brain, stripped of meninges, snap frozen and RNA extracted...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27693454/decorin-interacting-network-a-comprehensive-analysis-of-decorin-binding-partners-and-their-versatile-functions
#9
Maria A Gubbiotti, Sylvain D Vallet, Sylvie Ricard-Blum, Renato V Iozzo
Decorin, a prototype small leucine-rich proteoglycan, regulates a vast array of cellular processes including collagen fibrillogenesis, wound repair, angiostasis, tumor growth, and autophagy. This functional versatility arises from a wide array of decorin/protein interactions also including interactions with its single glycosaminoglycan side chain. The decorin-binding partners encompass numerous categories ranging from extracellular matrix molecules to cell surface receptors to growth factors and enzymes. Despite the diversity of the decorin interacting network, two main roles emerge as prominent themes in decorin function: maintenance of cellular structure and outside-in signaling, culminating in anti-tumorigenic effects...
September 2016: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/27643237/ish-nia-os-06-putative-mechanisms-for-cerebrovascular-remodelling-in-the-spontaneously-hypertensive-rat-shr-using-transcriptomics
#10
Dawid Walas, Karol Nowicki-Osuch, Dominic Alibhai, Julian Paton
OBJECTIVE: Cerebrovascular remodeling in the SHR may be causative to the known brainstem hypoperfusion. Using RNA sequencing, we examined age-related processes that may govern remodeling of the cerebral arteries in the SHR. DESIGN AND METHOD: In SHR and their progenitor (normotensive) control (Wistar Kyoto, WKY), RNA-seq was performed at three ages: 5, 9, 13 weeks old. Cerebral arteries were flushed and peeled off the brain, stripped of meninges, snap frozen and RNA extracted...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27586416/pleiotropic-roles-of-the-matricellular-protein-sparc-in-tendon-maturation-and-ageing
#11
Renate Gehwolf, Andrea Wagner, Christine Lehner, Amy D Bradshaw, Cornelia Scharler, Justyna A Niestrawska, Gerhard A Holzapfel, Hans-Christian Bauer, Herbert Tempfer, Andreas Traweger
Acute and chronic tendinopathies remain clinically challenging and tendons are predisposed to degeneration or injury with age. Despite the high prevalence of tendon disease in the elderly, our current understanding of the mechanisms underlying the age-dependent deterioration of tendon function remains very limited. Here, we show that Secreted protein acidic and rich in cysteine (Sparc) expression significantly decreases in healthy-aged mouse Achilles tendons. Loss of Sparc results in tendon collagen fibrillogenesis defects and Sparc-/- tendons are less able to withstand force in comparison with their respective wild type counterparts...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27519293/detection-of-type-i-collagen-fibrils-formation-and-dissociation-by-a-fluorescence-method-based-on-thioflavin-t
#12
Meilian Zou, Huan Yang, Haibo Wang, Haiyin Wang, Juntao Zhang, Benmei Wei, Hanjun Zhang, Dong Xie
In this study, fibrillogenesis and thermal dissociation of pepsin-soluble collagen (PSC), extracted from snakehead (Channa argus) skin, were monitored by fluorescence method based on thioflavin T (Th-T), where the accuracy and sensitivity were evaluated and compared with those of turbidity assay. The fluorescence method revealed the fibrillogenesis dynamics of collagen with better sensitivity, especially at nucleation and plateau stages. The melting temperature (Tm) of PSC was estimated to be 47°C by circular dichroism spectroscopy; below this temperature, the triple-helical structure should be intact...
November 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27484779/control-of-fibrotic-changes-through-the-synergistic-effects-of-anti-fibronectin-antibody-and-an-rgds-tagged-form-of-the-same-antibody
#13
Anil Tiwari, Rajendra Kumar, Jagat Ram, Maryada Sharma, Manni Luthra-Guptasarma
TGF-β and myofibroblasts play a key role in fibrosis, characterized by aberrant synthesis and deposition of extracellular matrix (ECM) proteins, such as fibronectin (Fn) and collagen type I. There are two major roles played by integrins in the fibrotic pathology: (i) Fn-integrin interaction, coupled with cytokines like TGF-β, facilitates the self-polymerization of Fn and regulates cell-matrix fibrillar adhesions, thereby promoting fibrillogenesis; (ii) Integrin interaction with an RGD (arginine-glycine-aspartic) consensus sequence in the latent TGF-β, resulting in its activation...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27375477/tendon-extracellular-matrix-alterations-in-ullrich-congenital-muscular-dystrophy
#14
Francesca Sardone, Francesco Traina, Alice Bondi, Luciano Merlini, Spartaco Santi, Nadir Mario Maraldi, Cesare Faldini, Patrizia Sabatelli
Collagen VI (COLVI) is a non-fibrillar collagen expressed in skeletal muscle and most connective tissues. Mutations in COLVI genes cause two major clinical forms, Bethlem myopathy and Ullrich congenital muscular dystrophy (UCMD). In addition to congenital muscle weakness, patients affected by COLVI myopathies show axial and proximal joint contractures and distal joint hypermobility, which suggest the involvement of the tendon function. We examined a peroneal tendon biopsy and tenocyte culture of a 15-year-old patient affected by UCMD with compound heterozygous COL6A2 mutations...
2016: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/27234502/periostin-as-a-multifunctional-modulator-of-the-wound-healing-response
#15
REVIEW
John T Walker, Karrington McLeod, Shawna Kim, Simon J Conway, Douglas W Hamilton
During tissue healing, the dynamic and temporal alterations required for effective repair occur in the structure and composition of the extracellular matrix (ECM). Matricellular proteins (MPs) are a group of diverse non-structural ECM components that bind cell surface receptors mediating interactions between the cell and its microenviroment, effectively regulating adhesion, migration, proliferation, signaling, and cell phenotype. Periostin (Postn), a pro-fibrogenic secreted glycoprotein, is defined as an MP based on its expression pattern and regulatory roles during development and healing and in disease processes...
September 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27162200/the-collagen-type-i-segment-long-spacing-sls-and-fibrillar-forms-formation-by-atp-and-sulphonated-diazo-dyes
#16
J Robin Harris, Richard J Lewis
The collagen type I segment long spacing (SLS) crystallite is a well-ordered rod-like molecular aggregate, ∼300nm in length, which is produced in vitro under mildly acidic conditions (pH 2.5-3.5) in the presence of 1mM ATP. The formation of the SLS crystallite amplifies the inherent linear structural features of individual collagen heterotrimers, due to the punctate linear distribution and summation of the bulkier amino acid side chains along the length of individual collagen heterotrimers. This can be correlated structurally with the 67nm D-banded collagen fibril that is found in vivo, and formed in vitro...
July 2016: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/27115146/decellularized-aortic-conduits-could-their-cryopreservation-affect-post-implantation-outcomes-a-morpho-functional-study-on-porcine-homografts
#17
Michele Gallo, Antonella Bonetti, Helen Poser, Filippo Naso, Tomaso Bottio, Roberto Bianco, Adolfo Paolin, Paolo Franci, Roberto Busetto, Anna Chiara Frigo, Edward Buratto, Michele Spina, Maurizio Marchini, Fulvia Ortolani, Laura Iop, Gino Gerosa
Decellularized porcine aortic valve conduits (AVCs) implanted in a Vietnamese Pig (VP) experimental animal model were matched against decellularized and then cryopreserved AVCs to assess the effect of cryopreservation on graft hemodynamic performance and propensity to in vivo repopulation by host's cells. VPs (n = 12) underwent right ventricular outflow tract substitution using AVC allografts and were studied for 15-month follow-up. VPs were randomized into two groups, receiving AVCs treated with decellularization alone (D; n = 6) or decellularization/cryopreservation (DC; n = 6), respectively...
November 2016: Heart and Vessels
https://www.readbyqxmd.com/read/26899371/high-throughput-proteomics-reveal-alarmins-as-amplifiers-of-tissue-pathology-and-inflammation-after-spinal-cord-injury
#18
Athanasios Didangelos, Michele Puglia, Michaela Iberl, Candela Sanchez-Bellot, Bernd Roschitzki, Elizabeth J Bradbury
Spinal cord injury is characterized by acute cellular and axonal damage followed by aggressive inflammation and pathological tissue remodelling. The biological mediators underlying these processes are still largely unknown. Here we apply an innovative proteomics approach targeting the enriched extracellular proteome after spinal cord injury for the first time. Proteomics revealed multiple matrix proteins not previously associated with injured spinal tissue, including small proteoglycans involved in cell-matrix adhesion and collagen fibrillogenesis...
February 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/26818589/myofibroblast-secretome-and-its-auto-paracrine-signaling
#19
REVIEW
Ritin Bomb, Mark R Heckle, Yao Sun, Salvatore Mancarella, Ramareddy V Guntaka, Ivan C Gerling, Karl T Weber
Myofibroblasts (myoFb) are phenotypically transformed, contractile fibroblast-like cells expressing α-smooth muscle actin microfilaments. They are integral to collagen fibrillogenesis with scar tissue formation at sites of repair irrespective of the etiologic origins of injury or tissue involved. MyoFb can persist long after healing is complete, where their ongoing turnover of collagen accounts for a progressive structural remodeling of an organ (a.k.a. fibrosis, sclerosis or cirrhosis). Such persistent metabolic activity is derived from a secretome consisting of requisite components in the de novo generation of angiotensin (Ang) II...
2016: Expert Review of Cardiovascular Therapy
https://www.readbyqxmd.com/read/26711913/forelimb-contractures-and-abnormal-tendon-collagen-fibrillogenesis-in-fibulin-4-null-mice
#20
Dessislava Z Markova, Te-Cheng Pan, Rui-Zhu Zhang, Guiyun Zhang, Takako Sasaki, Machiko Arita, David E Birk, Mon-Li Chu
Fibulin-4 is an extracellular matrix glycoprotein essential for elastic fiber formation. Mice deficient in fibulin-4 die perinatally because of severe pulmonary and vascular defects associated with the lack of intact elastic fibers. Patients with fibulin-4 mutations demonstrate similar defects, and a significant number die shortly after birth or in early childhood from cardiopulmonary failure. The patients also demonstrate skeletal and other systemic connective tissue abnormalities, including joint laxity and flexion contractures of the wrist...
June 2016: Cell and Tissue Research
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