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Collagen Fibrillogenesis

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https://www.readbyqxmd.com/read/28422173/critical-involvement-of-zeb2-in-collagen-fibrillogenesis-the-molecular-similarity-between-mowat-wilson-syndrome-and-ehlers-danlos-syndrome
#1
Mika Teraishi, Mikiro Takaishi, Kimiko Nakajima, Mitsunori Ikeda, Yujiro Higashi, Shinji Shimoda, Yoshinobu Asada, Atsushi Hijikata, Osamu Ohara, Yoko Hiraki, Seiji Mizuno, Toshiyuki Fukada, Takahisa Furukawa, Nobuaki Wakamatsu, Shigetoshi Sano
Mowat-Wilson syndrome (MOWS) is a congenital disease caused by de novo heterozygous loss of function mutations or deletions of the ZEB2 gene. MOWS patients show multiple anomalies including intellectual disability, a distinctive facial appearance, microcephaly, congenital heart defects and Hirschsprung disease. However, the skin manifestation(s) of patients with MOWS has not been documented in detail. Here, we recognized that MOWS patients exhibit many Ehlers-Danlos syndrome (EDS)-like symptoms, such as skin hyperextensibility, atrophic scars and joint hypermobility...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28412645/a-hidden-structural-vulnerability-in-the-thrombospondin-2-deficient-aorta-increases-the-propensity-to-intramural-delamination
#2
C Bellini, N J Kristofik, M R Bersi, T R Kyriakides, J D Humphrey
Mice lacking thrombospondin-2 (TSP2) represent an animal model of impaired collagen fibrillogenesis. Collagen constitutes ~1/3 of the wall of the normal murine descending thoracic aorta (DTA) and is thought to confer mechanical strength at high pressures. Microstructural analysis of the DTA from TSP2-null mice revealed irregular and disorganized collagen fibrils in the adventitia and at the interface between the media and adventitia. Yet, biaxial mechanical tests performed under physiologic loading conditions showed that most mechanical metrics, including stress and stiffness, were not different between mutant and control DTAs at 20- and 40-weeks of age, thus suggesting that the absence of TSP2 is well compensated under normal conditions...
March 9, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28411451/anisotropic-tough-double-network-hydrogel-from-fish-collagen-and-its-spontaneous-in%C3%A2-vivo-bonding-to-bone
#3
Md Tariful Islam Mredha, Nobuto Kitamura, Takayuki Nonoyama, Susumu Wada, Keiko Goto, Xi Zhang, Tasuku Nakajima, Takayuki Kurokawa, Yasuaki Takagi, Kazunori Yasuda, Jian Ping Gong
Soft supporting tissues in the human body, such as cartilages and ligaments, are tough materials and firmly fixed to bones. These soft tissues, once injured, cannot regenerate spontaneously in vivo. Developing tough and biocompatible hydrogels as artificial soft supporting tissues would substantially improve outcomes after soft tissue injury. Collagen is the main rigid component in soft connective tissues which is organized in various hierarchical arrays. We have successfully developed a novel class of collagen fibril-based tough hydrogels based on the double network (DN) concept using swim bladder collagen (SBC) extracted from Bester sturgeon fish...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28387435/collagen-v-haploinsufficiency-in-a-murine-model-of-classic-ehlers-danlos-syndrome-is-associated-with-deficient-structural-and-mechanical-healing-in-tendons
#4
Jessica M Johnston, Brianne K Connizzo, Snehal S Shetye, Kelsey A Robinson, Julianne Huegel, Ashley B Rodriguez, Mei Sun, Sheila M Adams, David E Birk, Louis J Soslowsky
Classic Ehlers-Danlos syndrome (EDS) patients suffer from connective tissue hyperelasticity, joint instability, skin hyperextensibility, tissue fragility, and poor wound healing due to heterozygous mutations in COL5a1 or COL5a2 genes. This study investigated the roles of collagen V in establishing structure and function in uninjured patellar tendons as well as in the injury response using a Col5a1(+/-) mouse, a model for classic EDS. These analyses were done comparing tendons from a classic EDS model (Col5a1(+/-) ) with wild-type controls...
April 7, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28300434/effect-of-carboxymethylcellulose-on-fibril-formation-of-collagen-in-vitro
#5
Cuicui Ding, Ronghui Shi, Zhigong Zheng, Min Zhang
The effect of carboxymethylcellulose (CMC) on the fibril formation of collagen in vitro was studied by turbidity measurements and atomic force microscopy (AFM). The kinetics curves of fibril formation indicated that the rate of collagen fibrillogenesis was decreased with the addition of CMC, meanwhile the final turbidity was obviously increased as the ratio of CMC/collagen reached 30%. The AFM images of collagen-CMC solutions showed that the number of nucleation sites of collagen fibrillogenesis was significantly increased with the presence of CMC, while the diameter of immature collagen fibrils was obviously decreased...
March 16, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28263412/molecular-dynamics-simulations-on-networks-of-heparin-and-collagen
#6
Martin Kulke, Norman Geist, Wenke Friedrichs, Walter Langel
Synthetic scaffolds containing collagen (Type I) are of increasing interest for bone tissue engineering, especially for highly porous biomaterials in combination with glycosaminoglycans. In experiments the integration of heparin during the fibrillogenesis resulted in different types of collagen fibrils, but models for this aggregation on a molecular scale were only tentative. We conducted molecular dynamic simulations investigating the binding of heparin to collagen and the influence of the telopeptides during collagen aggregation...
March 6, 2017: Proteins
https://www.readbyqxmd.com/read/28216298/cell-instructive-starpeg-heparin-collagen-composite-matrices
#7
Marcus Binner, Laura J Bray, Jens Friedrichs, Uwe Freudenberg, Mikhail V Tsurkan, Carsten Werner
Polymer hydrogels can be readily modulated with regard to their physical properties and functionalized to recapitulate molecular cues of the extracellular matrix (ECM). However, they remain structurally different from the hierarchical supramolecular assemblies of natural ECM. Accordingly, we herein report a set of hydrogel composite materials made from starPEG-peptide conjugates, maleimide-functionalized heparin and collagen type I that combine semisynthetic and ECM-derived components. Collagen fibrillogenesis was controlled by temperature and collagen concentration to form collagen microstructures which were then homogeneously distributed within the 3D composite matrix during hydrogel formation...
February 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28192200/the-binding-capacity-of-%C3%AE-1%C3%AE-1-%C3%AE-2%C3%AE-1-and-%C3%AE-10%C3%AE-1-integrins-depends-on-non-collagenous-surface-macromolecules-rather-than-the-collagens-in-cartilage-fibrils
#8
Christian Woltersdorf, Melanie Bonk, Birgit Leitinger, Mikko Huhtala, Jarmo Käpylä, Jyrki Heino, Christian Gil Girol, Stephan Niland, Johannes A Eble, Peter Bruckner, Rita Dreier, Uwe Hansen
Interactions of cells with supramolecular aggregates of the extracellular matrix (ECM) are mediated, in part, by cell surface receptors of the integrin family. These are important molecular components of cell surface-suprastructures regulating cellular activities in general. A subfamily of β1-integrins with von Willebrand-factor A-like domains (I-domains) in their α-chains can bind to collagen molecules and, therefore, are considered as important cellular mechano-receptors. Here we show that chondrocytes strongly bind to cartilage collagens in the form of individual triple helical molecules but very weakly to fibrils formed by the same molecules...
February 10, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28181591/lumican-peptides-rational-design-targeting-alk5-tgfbri
#9
Tarsis Ferreira Gesteira, Vivien J Coulson-Thomas, Yong Yuan, Jianhua Zhang, Helena B Nader, Winston W-Y Kao
Lumican, a small leucine rich proteoglycan (SLRP), is a component of extracellular matrix which also functions as a matrikine regulating multiple cell activities. In the cornea, lumican maintains corneal transparency by regulating collagen fibrillogenesis, promoting corneal epithelial wound healing, regulating gene expression and maintaining corneal homeostasis. We have recently shown that a peptide designed from the 13 C-terminal amino acids of lumican (LumC13) binds to ALK5/TGFBR1 (type1 receptor of TGFβ) to promote wound healing...
February 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28146148/anisotropically-organized-three-dimensional-culture-platform-for-reconstruction-of-a-hippocampal-neural-network
#10
So Hyun Kim, Sun-Kyoung Im, Soo-Jin Oh, Sohyeon Jeong, Eui-Sung Yoon, C Justin Lee, Nakwon Choi, Eun-Mi Hur
In native tissues, cellular and acellular components are anisotropically organized and often aligned in specific directions, providing structural and mechanical properties for actuating biological functions. Thus, engineering alignment not only allows for emulation of native tissue structures but might also enable implementation of specific functionalities. However, achieving desired alignment is challenging, especially in three-dimensional constructs. By exploiting the elastomeric property of polydimethylsiloxane and fibrillogenesis kinetics of collagen, here we introduce a simple yet effective method to assemble and align fibrous structures in a multi-modular three-dimensional conglomerate...
February 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/28127105/fibromodulin-expression-in-folliculostellate-cells-and-pericytes-is-promoted-by-tgf%C3%AE-signaling-in-rat-anterior-pituitary-gland
#11
Rahimi Syaidah, Takehiro Tsukada, Morio Azuma, Kotaro Horiguchi, Ken Fujiwara, Motoshi Kikuchi, Takashi Yashiro
Fibromodulin belongs to the family of small leucine-rich proteoglycans (SLRPs), an active component of extracellular matrix. It directly binds collagens to promote fibrillogenesis and also binds transforming growth factor-beta (TGFβ) to antagonize its actions. Our previous studies of rat anterior pituitary gland revealed that fibromodulin is expressed in folliculostellate cells and pericytes. Although our recent study showed that TGFβ2 secreted from folliculostellate cells induces collagen synthesis in pericytes, the involvement of fibromodulin in TGFβ2-mediated collagen regulation has not been studied...
December 28, 2016: Acta Histochemica et Cytochemica
https://www.readbyqxmd.com/read/28102596/delineation-of-ehlers-danlos-syndrome-phenotype-due-to-the-c-934c-t-p-arg312cys-mutation-in-col1a1-report-on-a-three-generation-family-without-cardiovascular-events-and-literature-review
#12
Marina Colombi, Chiara Dordoni, Marina Venturini, Arianna Zanca, Piergiacomo Calzavara-Pinton, Marco Ritelli
Classical Ehlers-Danlos syndrome (cEDS) is a rare connective tissue disorder primarily characterized by hyperextensible skin, defective wound healing, abnormal scars, easy bruising, and generalized joint hypermobility; arterial dissections are rarely observed. Mutations in COL5A1 and COL5A2 encoding type V collagen account for more than 90% of the patients so far characterized. In addition, cEDS phenotype was reported in a small number of patients carrying the c.934C>T mutation in COL1A1 that results in an uncommon substitution of a non-glycine residue in one Gly-Xaa-Yaa repeat of the pro-α1(I)-chain p...
February 2017: American Journal of Medical Genetics. Part A
https://www.readbyqxmd.com/read/28042273/gingival-crevicular-fluid-periostin-levels-in-chronic-periodontitis-patients-following-nonsurgical-periodontal-treatment-with-low-level-laser-therapy
#13
Dhanangchaayan Kumaresan, Aruna Balasundaram, Vanaja Krishna Naik, Deva Priya Appukuttan
OBJECTIVE: Periostin is a matricellular protein highly expressed in periosteum, periodontal ligament and is essential for tissue integrity and maturation. It plays a role in collagen fibrillogenesis and is downregulated in periodontal disease. Biostimulation utilizing low-level laser therapy (LLLT) influences periodontal ligament fibroblast proliferation. This study was conducted with the objective of estimating periostin levels in chronic periodontitis (CP) patients following LLLT as an adjunct to root surface debridement (RSD)...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28017821/identification-of-a-p-arg708gln-variant-in-col1a2-in-atypical-femoral-fractures
#14
Thomas Funck-Brentano, Agnes Ostertag, Francoise Debiais, Patrice Fardellone, Corinne Collet, Etienne Mornet, Martine Cohen-Solal
OBJECTIVES: Long-term bisphosphonates exposure is a proven risk factor for atypical femoral fractures (AFF) but several cases occur in untreated patients. The identification of other risk factors for AFF is critical for the management of osteoporosis. We here assessed the genetic factors associated with AFF regardless of the treatment. METHODS: Cases were identified through ICD-10 codes in 3 academic centers. Medical records were analyzed by 2 investigators that adjudicated X-rays for typical or atypical fractures...
December 23, 2016: Joint, Bone, Spine: Revue du Rhumatisme
https://www.readbyqxmd.com/read/28011632/a-developmental-transcriptomic-analysis-of-pax1-and-pax9-in-embryonic-intervertebral-disc-development
#15
V Sivakamasundari, Petra Kraus, Wenjie Sun, Xiaoming Hu, Siew Lan Lim, Shyam Prabhakar, Thomas Lufkin
Pax1 and Pax9 play redundant, synergistic functions in the patterning and differentiation of the sclerotomal cells that give rise to the vertebral bodies and intervertebral discs (IVD) of the axial skeleton. They are conserved in mice and humans, whereby mutation/deficiency of human PAX1/PAX9 has been associated with kyphoscoliosis. By combining cell-type-specific transcriptome and ChIP-sequencing data, we identified the roles of Pax1/Pax9 in cell proliferation, cartilage development and collagen fibrillogenesis, which are vital in early IVD morphogenesis...
February 15, 2017: Biology Open
https://www.readbyqxmd.com/read/27928505/collagen-a-network-for-regenerative-medicine
#16
REVIEW
K M Pawelec, S M Best, R E Cameron
The basic building block of the extra-cellular matrix in native tissue is collagen. As a structural protein, collagen has an inherent biocompatibility making it an ideal material for regenerative medicine. Cellular response, mediated by integrins, is dictated by the structure and chemistry of the collagen fibers. Fiber formation, via fibrillogenesis, can be controlled in vitro by several factors: pH, ionic strength, and collagen structure. After formation, fibers are stabilized via cross-linking. The final bioactivity of collagen scaffolds is a result of both processes...
October 28, 2016: Journal of Materials Chemistry. B, Materials for Biology and Medicine
https://www.readbyqxmd.com/read/27926839/circular-dichroism-spectroscopy-of-collagen-fibrillogenesis-a-new-use-for-an-old-technique
#17
Kathryn E Drzewiecki, Daniel R Grisham, Avanish S Parmar, Vikas Nanda, David I Shreiber
Type-I collagen assembles in a stepwise, hierarchic fashion from the folding of the triple helix to the assembly of fibrils into fibers. The mature assembled fibers are crucial for tissue structure and mechanics, cell interactions, and other functions in vivo. Although triple helix folding can be followed with the use of optical methods such as circular dichroism (CD) spectroscopy, fibrillogenesis is typically measured by alternative methods such as turbidity, rheology, and microscopy. Together, these approaches allow for investigation of the mechanical properties and architectures of collagen-based scaffolds and excised tissues...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27770943/insight-into-the-collagen-assembly-in-the-presence-of-lysine-and-glutamic-acid-an-in-vitro-study
#18
Xinhua Liu, Nianhua Dan, Weihua Dan
The aim of this study is to evaluate the effects of two different charged amino acids in collagen chains, lysine and glutamic acid, on the fibrillogenesis process of collagen molecules. The turbidity, zeta potential, and fiber diameter analysis suggest that introducing the positively charged lysine into collagen might improve the sizes or amounts of the self-assembled collagen fibrils significantly. Conversely, the negatively charged glutamic acid might restrict the self-assembly of collagen building blocks into a higher order structure...
January 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27760365/nanoscale-imaging-of-collagen-gels-with-focused-ion-beam-milling-and-scanning-electron-microscopy
#19
Shawn P Reese, Niloofar Farhang, Randy Poulson, Gennie Parkman, Jeffrey A Weiss
In vitro polymerized type I collagen hydrogels have been used extensively as a model system for three-dimensional (3D) cell and tissue culture, studies of fibrillogenesis, and investigation of multiscale force transmission within connective tissues. The nanoscale organization of collagen fibrils plays an essential role in the mechanics of these gels and emergent cellular behavior in culture, yet quantifying 3D structure with nanoscale resolution to fully characterize fibril organization remains a significant technical challenge...
October 18, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27753891/ish-nia-os-06-putative-mechanisms-for-cerebrovascular-remodelling-in-the-spontaneously-hypertensive-rat-shr-using-transcriptomics
#20
Dawid Walas, Karol Nowicki-Osuch, Dominic Alibhai, Julian Paton
OBJECTIVE: Cerebrovascular remodeling in the SHR may be causative to the known brainstem hypoperfusion. Using RNA sequencing, we examined age-related processes that may govern remodeling of the cerebral arteries in the SHR. DESIGN AND METHOD: In SHR and their progenitor (normotensive) control (Wistar Kyoto, WKY), RNA-seq was performed at three ages: 5, 9, 13 weeks old. Cerebral arteries were flushed and peeled off the brain, stripped of meninges, snap frozen and RNA extracted...
September 2016: Journal of Hypertension
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