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Electroporation

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https://www.readbyqxmd.com/read/28087631/papc-couples-the-segmentation-clock-to-somite-morphogenesis-by-regulating-n-cadherin-dependent-adhesion
#1
Jérome Chal, Charlène Guillot, Olivier Pourquié
Vertebrate segmentation is characterized by the periodic formation of epithelial somites from the mesenchymal presomitic mesoderm (PSM). How the rhythmic signaling pulse delivered by the Segmentation Clock is translated into the periodic morphogenesis of somites remains poorly understood. Here, we focused on the role of Paraxial protocadherin (PAPC/Pcdh8) in this process. We showed that in chicken and mouse embryos, PAPC expression is tightly regulated by the Clock and Wavefront system in the posterior PSM...
January 13, 2017: Development
https://www.readbyqxmd.com/read/28078482/irreversible-electroporation-as-treatment-of-locally-advanced-and-as-margin-accentuation-in-borderline-resectable-pancreatic-adenocarcinoma
#2
P Marsanic, A Mellano, A Sottile, M De Simone
In recent years, many local ablation technologies based on thermal damage have been used in the treatment of locally advanced pancreatic carcinoma (LAPC) and borderline resectable pancreatic carcinoma (BLRPC). However, they are associated with major complications because of possible vascular and ductal damage. Irreversible electroporation (IRE) is a nonthermal ablation technology that seems safe near vital vascular and ductal structures. IRE could be used as exclusive treatment of LAPC (en situ to IRE) after induction chemotherapy In BLRPC, surgery is not really radical in 6% of patients (microscopic residual) and local recurrences occur in 11-42% of apparent radical resections...
January 11, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/28070536/establishment-a-cho-cell-line-expressing-human-cd52-molecule
#3
Tati Kadijeh, Yazdanpanah-Samani Mahsa, Ramezani Amin, Mahmoudi Maymand Elham, Ghaderi Abbas
BACKGROUND: CD52 is a small glycoprotein with a GPI anchor at its C-terminus. CD52 is expressed by Normal and malignant T and B lymphocytes and monocytes. There are detectable amounts of soluble CD52 in plasma of patients with CLL and could be used as a tumor marker. Although the biological function of CD52 is unknown but it seems that CD52 may be involved in migration and activation of T-cells .The aim of this study was to clone and express human CD52 gene in CHO cell line and studying its function in more details...
October 2016: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28070106/excessive-activation-of-ahr-signaling-disrupts-neuronal-migration-in-the-hippocampal-ca1-region-in-the-developing-mouse
#4
Eiki Kimura, Ken-Ichiro Kubo, Toshihiro Endo, Kazunori Nakajima, Masaki Kakeyama, Chiharu Tohyama
The aryl hydrocarbon receptor (AhR) avidly binds dioxin, a ubiquitous environmental contaminant. Disruption of downstream AhR signaling has been reported to alter neuronal development, and rodent offspring exposed to dioxin during gestation and lactation showed abnormalities in learning and memory, emotion, and social behavior. However, the mechanism behind the disrupted AhR signaling and developmental neurotoxicity induced by xenobiotic ligands remains elusive. Therefore, we studied how excessive AhR activation affects neuronal migration in the hippocampal CA1 region of the developing mouse brain...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28067371/high-efficiency-hydrodynamic-bacterial-electrotransformation
#5
Paulo A Garcia, Zhifei Ge, Laura E Kelley, Steven J Holcomb, Cullen R Buie
Synthetic biology holds great potential for addressing pressing challenges for mankind and our planet. One technical challenge in tapping into the full potential of synthetic biology is the low efficiency and low throughput of genetic transformation for many types of cells. In this paper, we discuss a novel microfluidic system for improving bacterial electrotransformation efficiency and throughput. Our microfluidic system is comprised of non-uniform constrictions in microchannels to facilitate high electric fields with relatively small applied voltages to induce electroporation...
January 9, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28065834/nanosecond-pulsed-electric-fields-trigger-cell-differentiation-in-chlamydomonas-reinhardtii
#6
Fan Bai, Christian Gusbeth, Wolfgang Frey, Peter Nick
Nanosecond pulsed electric fields (nsPEFs) have great potential for biotechnological and medical applications. However, the biological mechanisms causing the cellular responses are still far from understood. We used the unicellular green algae Chlamydomonas reinhardtii as experimental model to dissect the immediate consequences of electroporation from the developmental cellular responses evoked by nsPEFs. We observe that nsPEFs induce a short-term permeabilization of the membrane, accompanied by swelling and oxidative burst...
January 5, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28062003/identification-of-genes-involved-in-mycoplasma-gallisepticum-biofilm-formation-using-mini-tn4001-sgm-transposon-mutagenesis
#7
Yang Wang, Li Yi, Fanqing Zhang, Xusheng Qiu, Lei Tan, Shengqing Yu, Xiangchao Cheng, Chan Ding
Mycoplasma gallisepticum (MG) is an important pathogen that can cause chronic respiratory disease in chickens and infectious sinusitis in turkeys. MG has the ability to form biofilms. The molecular mechanisms underlying MG biofilm formation are complex and poorly understood. To better understand the mechanisms involved in biofilm formation, mini-Tn4001-SGM, a novel transposon vector containing the gentamicin gene was constructed and electroporated into MG strain Rlow. Of the 738 mutants obtained, 12 had significantly reduced capacity to form biofilms in a polystyrene microtiter-plate biofilm assay...
January 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28060338/a-submerged-filter-paper-sandwich-for-long-term-ex-ovo-time-lapse-imaging-of-early-chick-embryos
#8
Manuel Schmitz, Ben K A Nelemans, Theodoor H Smit
Due to its availability, low cost, flat geometry, and transparency, the ex ovo chick embryo has become a major vertebrate animal model for the study of morphogenetic events, such as gastrulation(2), neurulation(3)(-)(5), somitogenesis(6), heart bending(7,8), and brain formation(9)(-)(13), during early embryogenesis. Key to understanding morphogenetic processes is to follow them dynamically by time-lapse imaging. The acquisition of time-lapse movies of chick embryogenesis ex ovo has been limited either to short time windows or to the need for an incubator to control temperature and humidity around the embryo(14)...
December 28, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28058692/comparative-characteristics-of-two-anion-channel-rhodopsins-and-prospects-of-their-use-in-optogenetics
#9
D A Dolgikh, A Yu Malyshev, M V Roshchin, G R Smirnova, O V Nekrasova, L E Petrovskaya, T B Feldman, P M Balaban, M P Kirpichnikov, M A Ostrovsky
Anion-selective opsins slow ChloC and ACR2 were expressed in rat brain cortical neurons by electroporation in utero. It is shown that the light-activated channel ACR2 has pronounced advantages in terms of both the inactivation kinetics and the neuron inhibition intensity, which is associated with a more negative value of the light-activated current reversal potential compared to the slow ChloC channel. The identified properties of opsin ACR2 indicate that it can be used for strictly controlled suppression of neuronal activity in optogenetic experiments, including the expression in the retinal ganglionic cells for reconstituting the OFF-component of their receptive field, which is essential for optogenetic prosthetics of degenerative retina...
November 2016: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28052102/irreversible-electroporation-of-the-pancreas-using-parallel-plate-electrodes-in-a-porcine-model-a-feasibility-study
#10
Steffi J E Rombouts, Maarten W Nijkamp, Willemijn P M van Dijck, Lodewijk A A Brosens, Maurits Konings, R van Hillegersberg, Inne H M Borel Rinkes, Jeroen Hagendoorn, Fred H Wittkampf, I Quintus Molenaar
BACKGROUND: Irreversible electroporation (IRE) with needle electrodes is being explored as treatment option in locally advanced pancreatic cancer. Several studies have shown promising results with IRE needles, positioned around the tumor to achieve tumor ablation. Disadvantages are the technical difficulties for needle placement, the time needed to achieve tumor ablation, the risk of needle track seeding and most important the possible occurrence of postoperative pancreatic fistula via the needle tracks...
2017: PloS One
https://www.readbyqxmd.com/read/28049588/efficacy-of-an-autophagy-targeted-dna-vaccine-against-avian-leukosis-virus-subgroup-j
#11
Zhenkai Dai, Jianfei Huang, Xiaoya Lei, Yiming Yan, Piaopiao Lu, Huanmin Zhang, Wencheng Lin, Weiguo Chen, Jingyun Ma, Qingmei Xie
Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85+rapamycin and pVAX1-gp85-LC3+rapamycin through electroporation...
December 31, 2016: Vaccine
https://www.readbyqxmd.com/read/28046204/electroporation-in-the-rodent-embryonic-brain-using-whole-embryo-culture-system
#12
Takako Kikkawa, Masanori Takahashi, Noriko Osumi
This unit describes basic methods for mammalian whole embryo culture (WEC) using embryonic day 10.5 mouse embryos, including the preparation of high-quality immediately centrifuged (IC) rat serum that is commonly used for WEC and is essential for normal growth and development of cultured mouse and rat embryos in vitro. An alternative protocol for different stages of rodent embryos is also introduced. Since embryos for WEC are dissected out of the uterus and manipulated under the microscope, one can overcome many of the difficulties of gene delivery encountered using in utero electroporation...
January 3, 2017: Current Protocols in Neuroscience
https://www.readbyqxmd.com/read/28045587/conjugation-of-%C3%AF-bt1-derived-integrative-plasmid-pdzl802-in-amycolatopsis-mediterranei-u32
#13
Chen Li, Li Zhou, Ying Wang, Guoping Zhao, Xiaoming Ding
The genus Amycolatopsis is well known for its ability to produce antibiotics, and an increasing number of valuable biotechnological applications, such as bioremediation, biodegradation, bioconversion, and potentially biofuel, that employ this genus have been developed. Amycolatopsis mediterranei is an industrial-scale producer of the important antibiotic rifamycin, which plays a vital role in antimycobacterial therapy. Genetic studies of Amycolatopsis species have progressed slowly due to the lack of efficient transformation methods and stable plasmid vectors...
January 3, 2017: Bioengineered
https://www.readbyqxmd.com/read/28042055/development-of-an-efficient-electroporation-method-for-rhizobacterial-bacillus-mycoides-strains
#14
Yanglei Yi, Oscar P Kuipers
In order to develop a method for electroporation of environmental Bacillus mycoides strains, we optimized several conditions that affect the electroporation efficiency of this bacterium. By combining the optimized conditions, the electroporation efficiency of strain EC18 was improved to (1.3±0.6)×10(5)cfu/μg DNA, which is about 10(3)-fold increase in comparison with a previously reported value. The method was further validated on various B. mycoides strains, yielding reasonable transformation efficiencies...
December 30, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28035048/live-cell-single-molecule-labeling-and-analysis-of-myosin-motors-with-quantum-dots
#15
Hiroyasu Hatakeyama, Yoshihito Nakahata, Hirokazu Yarimizu, Makoto Kanzaki
Quantum dots (QDs) are a powerful tool for quantitatively analyzing dynamic cellular processes by single-particle tracking. However, tracking of intracellular molecules with QDs is limited by their inability to penetrate the plasma membrane and bind to specific molecules of interest. Although several techniques for overcoming these problems have been proposed, they are either complicated or inconvenient. To address this issue, in this study, we developed a simple, convenient, and nontoxic method for labeling intracellular molecules in cells using HaloTag technology and electroporation...
January 1, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28034769/sfpq-associates-to-lsd1-and-regulates-the-migration-of-newborn-pyramidal-neurons-in-the-developing-cerebral-cortex
#16
K Saud, J Cánovas, C I Lopez, F A Berndt, E López, J C Maass, A Barriga, M Kukuljan
The development of the cerebral cortex requires the coordination of multiple processes ranging from the proliferation of progenitors to the migration and establishment of connectivity of the newborn neurons. Epigenetic regulation carried out by the COREST/LSD1 complex has been identified as a mechanism that regulates the development of pyramidal neurons of the cerebral cortex. We now identify the association of the multifunctional RNA-binding protein SFPQ to LSD1 during the development of the cerebral cortex...
December 26, 2016: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/28033149/partial-gland-ablation-in-the-management-of-prostate-cancer-a-review
#17
Timothy D McClure, Daniel J A Margolis, Jim C Hu
PURPOSE OF REVIEW: The index lesion theory has created a strong interest in partial gland ablation for men with prostate cancer. By only treating the focus of clinically significant disease and avoidance of surrounding periprostatic tissue, one may provide adequate oncologic control with minimal side effects. Accurate identification of the index lesion and effective ablation are critical for satisfactory oncologic outcomes. Herein, we review key ablative techniques used in partial gland ablation...
December 28, 2016: Current Opinion in Urology
https://www.readbyqxmd.com/read/28033117/the-impact-of-electrode-resistance-on-the-biogalvanic-characterisation-technique
#18
J H Chandler, D A Head, M E Hubbard, A Neville, D G Jayne, P R Culmer
Measurement of a tissue-specific electrical resistance may offer a discriminatory metric for evaluation of tissue health during cancer surgery. With a move toward minimally-invasive procedures, applicable contact sensing modalities must be scalable, fast and robust. A passive resistance characterisation method utilising a biogalvanic cell as an intrinsic power source has been proposed as a potentially suitable solution. Previous work has evaluated this system with results showing effective discrimination of tissue type and damage (through electroporation)...
December 29, 2016: Physiological Measurement
https://www.readbyqxmd.com/read/28029459/real-time-monitoring-of-cytotoxic-effects-of-electroporation-on-breast-and-colon-cancer-cell-lines
#19
Danijela M Cvetković, Marko N Živanović, Milena G Milutinović, Tijana R Djukić, Miloš D Radović, Aleksandar M Cvetković, Nenad D Filipović, Nebojša D Zdravković
PURPOSE: To study the effects of electroporation on different cell lines. MATERIAL: The effects of electroporation on human breast cancer (MDA-MB-231), human colon cancer (SW-480 and HCT-116), human fibroblast cell line (MRC-5), primary human aortic smooth muscle cells (hAoSMC) and human umbilical vein endothelial cells (HUVEC) were studied. Real-time technology was used for cell viability monitoring. Acridine orange/ethidium bromide assay was applied for cell death type determination...
October 22, 2016: Bioelectrochemistry
https://www.readbyqxmd.com/read/28028534/cellular-uptake-and-dynamics-of-unlabeled-freestanding-silicon-nanowires
#20
John F Zimmerman, Ramya Parameswaran, Graeme Murray, Yucai Wang, Michael Burke, Bozhi Tian
The ability to seamlessly merge electronic devices with biological systems at the cellular length scale is an exciting prospect for exploring new fundamental cell biology and in designing next-generation therapeutic devices. Semiconductor nanowires are well suited for achieving this goal because of their intrinsic size and wide range of possible configurations. However, current studies have focused primarily on delivering substrate-bound nanowire devices through mechanical abrasion or electroporation, with these bulkier substrates negating many of the inherent benefits of using nanoscale materials...
December 2016: Science Advances
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