keyword
MENU ▼
Read by QxMD icon Read
search

Cd81

keyword
https://www.readbyqxmd.com/read/28934733/exosomes-from-human-induced-pluripotent-stem-cell-derived-mesenchymal-stromal-cells-hipsc-mscs-protect-liver-against-hepatic-ischemia-reperfusion-injury-via-activating-sphingosine-kinase-and-sphingosine-1-phosphate-signaling-pathway
#1
Yingdong Du, Dawei Li, Conghui Han, Haoyu Wu, Longmei Xu, Ming Zhang, Jianjun Zhang, Xiaosong Chen
BACKGROUND/AIMS: This study aimed to evaluate the effects of exosomes produced by human-induced pluripotent stem cell-derived mesenchymal stromal cells (hiPSC-MSCs-Exo) on hepatic ischemia-reperfusion (I/R) injury, as well as the underlying mechanisms. METHODS: Exosomes derived from hiPSC-MSCs were isolated and characterized both biochemically and biophysically. hiPSC-MSCs-Exo were injected systemically into a murine ischemia/reperfusion injury model via the inferior vena cava, and then the therapeutic effects were evaluated...
September 21, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28920599/magnetic-nanoparticle-enhanced-surface-plasmon-resonance-biosensor-for-extracellular-vesicle-analysis
#2
Agnes T Reiner, Nicolas-Guillermo Ferrer, Priyamvada Venugopalan, Ruenn Chai Lai, Sai Kiang Lim, Jakub Dostálek
The sensitive analysis of small lipid extracellular vesicles (EVs) by using a grating-coupled surface plasmon resonance (GC-SPR) biosensor has been reported. In order to enable the analysis of trace amounts of EVs present in complex liquid samples, the target analyte is pre-concentrated on the sensor surface by using magnetic nanoparticles and its affinity binding is probed by wavelength interrogation of SPR. The GC-SPR has been demonstrated to allow for the implementation of efficient pulling of EVs to the sensor surface by using magnetic nanoparticles and an external magnetic field gradient applied through the sensor chip...
September 18, 2017: Analyst
https://www.readbyqxmd.com/read/28903311/development-of-hepatoma-derived-bidirectional-oval-like-cells-as-a-model-to-study-host-interactions-with-hepatitis-c-virus-during-differentiation
#3
Masahiko Ito, Suofeng Sun, Takasuke Fukuhara, Ryosuke Suzuki, Miho Tamai, Toyohiko Yamauchi, Kenji Nakashima, Yoh-Ichi Tagawa, Shigetoshi Okazaki, Yoshiharu Matsuura, Takaji Wakita, Tetsuro Suzuki
Directed differentiation of human stem cells including induced pluripotent stem cells into hepatic cells potentially leads to acquired susceptibility to hepatitis C virus (HCV) infection. However, cellular determinants that change their expression during cell reprogramming or hepatic differentiation and are pivotal for supporting the HCV life cycle remain unclear. In this study, by introducing a set of reprogramming factors, we established HuH-7-derived oval-like cell lines, Hdo-17 and -23, which possess features of bipotential liver precursors...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28886485/development-of-an-epitope-based-vaccine-inhibiting-immune-cells-rolling-and-migration-against-atherosclerosis-using-in-silico-approaches
#4
Mehdi Tourani, Ahmad Karkhah, Ali Najafi
Atherosclerosis is a chronic inflammatory disease characterized by formation of pro-oxidative lipids in large and medium-sized vessels. Over the years, many treatments and drugs have entered the market to improve atherosclerosis and autoantigen-mediated active immunization is currently considered as a beneficial method. Therefore, this study was conducted to design a novel epitope-based vaccine against atherosclerosis employing CD99, CD81 and CD99L2 antigens. In this way, structural vaccinology approaches were used to design a novel multi-epitope vaccine against atherosclerosis...
September 1, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28880937/differential-expression-of-tetraspanin-superfamily-members-in-dendritic-cell-subsets
#5
Malou Zuidscherwoude, Kuntal Worah, Alie van der Schaaf, Sonja I Buschow, Annemiek B van Spriel
Dendritic cells (DCs), which are essential for initiating immune responses, are comprised of different subsets. Tetraspanins organize dendritic cell membranes by facilitating protein-protein interactions within the so called tetraspanin web. In this study we analyzed expression of the complete tetraspanin superfamily in primary murine (CD4+, CD8+, pDC) and human DC subsets (CD1c+, CD141+, pDC) at the transcriptome and proteome level. Different RNA and protein expression profiles for the tetraspanin genes across human and murine DC subsets were identified...
2017: PloS One
https://www.readbyqxmd.com/read/28878075/the-characterization-of-the-quasi-enveloped-hepatitis-e-virus-particles-released-by-the-cellular-exosomal-pathway
#6
Shigeo Nagashima, Masaharu Takahashi, Tominari Kobayashi, Tanggis, Tsutomu Nishizawa, Takashi Nishiyama, Putu Prathiwi Primadharsini, Hiroaki Okamoto
Our previous studies demonstrated that membrane-associated hepatitis E virus (HEV) particles-now considered "quasi-enveloped particles"-are present in the multivesicular body with intraluminal vesicles (exosomes) in infected cells, and that the release of HEV virions is related to the exosomal pathway. In this study, we characterized exosomes purified from culture supernatant of HEV-infected PLC/PRF/5 cells. Purified CD63-, CD9-, or CD81-positive exosomes derived from the culture supernatant of HEV-infected cells that had been cultivated in serum-free medium were found to contain HEV RNA and the viral capsid (ORF2) and ORF3 proteins, as determined by a RT-PCR and Western blotting, respectively...
September 6, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28871089/cd81-association-with-samhd1-enhances-hiv-1-reverse-transcription-by-increasing-dntp-levels
#7
Vera Rocha-Perugini, Henar Suárez, Susana Álvarez, Soraya López-Martín, Gina M Lenzi, Felipe Vences-Catalán, Shoshana Levy, Baek Kim, María A Muñoz-Fernández, Francisco Sánchez-Madrid, Maria Yáñez-Mó
In this study, we report that the tetraspanin CD81 enhances human immunodeficiency virus (HIV)-1 reverse transcription in HIV-1-infected cells. This is enabled by the direct interaction of CD81 with the deoxynucleoside triphosphate phosphohydrolase SAMHD1. This interaction prevents endosomal accumulation and favours the proteasome-dependent degradation of SAMHD1. Consequently, CD81 depletion results in SAMHD1 increased expression, decreasing the availability of deoxynucleoside triphosphates (dNTP) and thus HIV-1 reverse transcription...
September 4, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28840975/cardioprotection-mediated-by-exosomes-is-impaired-in-the-setting-of-type-ii-diabetes-but-can-be-rescued-by-the-use-of-non-diabetic-exosomes-in-vitro
#8
Sean M Davidson, Jaime A Riquelme, Kaloyan Takov, Jose M Vicencio, Claire Boi-Doku, Vanessa Khoo, Christian Doreth, Dina Radenkovic, Sergio Lavandero, Derek M Yellon
Many patients with ischaemic heart disease also have diabetes. As myocardial infarction is a major cause of mortality and morbidity in these patients, treatments that increase cell survival in response to ischaemia and reperfusion are needed. Exosomes-nano-sized, lipid vesicles released from cells-can protect the hearts of non-diabetic rats. We previously showed that exosomal HSP70 activates a cardioprotective signalling pathway in cardiomyocytes culminating in ERK1/2 and HSP27 phosphorylation. Here, we investigated whether the exosomal cardioprotective pathway remains intact in the setting of type II diabetes...
August 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28828654/extracellular-vesicle-isolation-and-analysis-by-western-blotting
#9
Emma J K Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, George M Church
Extracellular vesicles (EVs) are released by mammalian cells and are thought to be important mediators of intercellular communication. There are many methods for isolating EVs from cell culture media, but the most popular methods involve purification based on ultracentrifugation . Here, we provide a detailed protocol for isolating EVs by differential ultracentrifugation and analyzing EV proteins (such as the tetraspanins CD9 , CD63 and CD81 ) by western blotting.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28759649/the-tetraspanin-cd9-facilitates-mers-coronavirus-entry-by-scaffolding-host-cell-receptors-and-proteases
#10
James T Earnest, Michael P Hantak, Kun Li, Paul B McCray, Stanley Perlman, Tom Gallagher
Infection by enveloped coronaviruses (CoVs) initiates with viral spike (S) proteins binding to cellular receptors, and is followed by proteolytic cleavage of receptor-bound S proteins, which prompts S protein-mediated virus-cell membrane fusion. Infection therefore requires close proximity of receptors and proteases. We considered whether tetraspanins, scaffolding proteins known to facilitate CoV infections, hold receptors and proteases together on cell membranes. Using knockout cell lines, we found that the tetraspanin CD9, but not the tetraspanin CD81, formed cell-surface complexes of dipeptidyl peptidase 4 (DPP4), the MERS-CoV receptor, and the type II transmembrane serine protease (TTSP) member TMPRSS2, a CoV-activating protease...
July 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28721844/cd81-large-extracellular-loop-containing-fusion-proteins-with-a-dominant-negative-effect-on-hcv-cell-spread-and-replication
#11
Boyan Grigorov, Jennifer Molle, Eric Rubinstein, Fabien Zoulim, Birke Bartosch
The roles of CD81 in the hepatitis C virus (HCV) life cycle are multiple but remain ill characterized. CD81 is known to interact with the HCV glycoproteins as an attachment factor. It also has an important role in the post-attachment entry process. Its interaction with claudin-1, for example, is vital for viral uptake and trafficking. Furthermore, CD81 and its role in membrane organization and trafficking are thought to play a pivotal role in HCV replication. Some of these functions are particularly limited to human CD81; others can be substituted with CD81 molecules from other species...
July 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28708602/development-of-hepatoma-derived-bidirectional-oval-like-cells-as-a-model-to-study-host-interactions-with-hepatitis-c-virus-during-differentiation
#12
Masahiko Ito, Suofeng Sun, Takasuke Fukuhara, Ryosuke Suzuki, Miho Tamai, Toyohiko Yamauchi, Kenji Nakashima, Yoh-Ichi Tagawa, Shigetoshi Okazaki, Yoshiharu Matsuura, Takaji Wakita, Tetsuro Suzuki
Directed differentiation of human stem cells including induced pluripotent stem cells into hepatic cells potentially leads to acquired susceptibility to hepatitis C virus (HCV) infection. However, cellular determinants that change their expression during cell reprogramming or hepatic differentiation and are pivotal for supporting the HCV life cycle remain unclear. In this study, by introducing a set of reprogramming factors, we established HuH-7-derived oval-like cell lines, Hdo-17 and -23, which possess features of bipotential liver precursors...
July 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28694142/increased-psa-expression-on-prostate-cancer-exosomes-in-in%C3%A2-vitro-condition-and-in-cancer-patients
#13
Mariantonia Logozzi, Daniela F Angelini, Elisabetta Iessi, Davide Mizzoni, Rossella Di Raimo, Cristina Federici, Luana Lugini, Giovanna Borsellino, Alessandro Gentilucci, Federico Pierella, Vittorio Marzio, Alessandro Sciarra, Luca Battistini, Stefano Fais
Prostate specific antigen (PSA) test is the most common, clinically validated test for the diagnosis of prostate cancer (PCa). While neoplastic lesions of the prostate may cause aberrant levels of PSA in the blood, the quantitation of free or complexed PSA poorly discriminates cancer patients from those developing benign lesions, often leading to invasive and unnecessary surgical procedures. Microenvironmental acidity increases exosome release by cancer cells. In this study we evaluated whether acidity, a critical phenotype of malignancy, could influence exosome release and increase the PSA expression in nanovesicles released by PCa cells...
September 10, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28659476/entry-and-release-of-hepatitis-c-virus-in-polarized-human-hepatocytes
#14
Sandrine Belouzard, Adeline Danneels, Lucie Fénéant, Karin Séron, Yves Rouillé, Jean Dubuisson
Hepatitis C virus (HCV) infects primarily hepatocytes that are highly polarized cells. The relevance of cell polarity in the HCV life cycle has only been addressed in distant models and remains poorly understood. Although polarized epithelial cells have a rather simple morphology with a basolateral and an apical domain, hepatocytes exhibit complex polarization structures. However, it has been reported that some selected polarized HepG2 cell clones can exhibit a honeycomb pattern of distribution of the tight-junction proteins, typical of columnar polarized epithelia which can be used as a simple model to study the role of cell polarization in viral infection of hepatocytes...
June 28, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28643872/circulating-microrna-as-candidates-for-early-embryonic-viability-in-cattle
#15
Ky G Pohler, Jonathan A Green, Laura A Moley, Sumedha Gunewardena, Wei-Ting Hung, Rebecca R Payton, Xiaoman Hong, Lane K Christenson, Tom W Geary, Michael F Smith
Blood-borne extracellular vesicles (i.e., exosomes and microvesicles) carrying microRNAs (miRNAs) could make excellent biomarkers of disease and different physiologic states, including pregnancy status. We tested the hypothesis that circulating extracellular vesicle-derived miRNAs might differentiate the pregnancy status of cows that had maintained pregnancy to Day 30 from non-pregnant cows or from those that exhibited embryonic mortality between Days 17 and 30 of gestation. Cows were randomly assigned for artificial insemination with fertile semen (n = 36) or dead semen (n = 8; control group) on Day 0 (day of estrus)...
August 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28643202/specific-igm-and-regulation-of-antibody-responses
#16
Anna Sörman, Birgitta Heyman
Specific IgM, administered together with the antigen it recognizes, enhances primary antibody responses, formation of germinal centers, and priming for secondary antibody responses. The response to all epitopes on the antigen to which IgM binds is usually enhanced. IgM preferentially enhances responses to large antigens such as erythrocytes, malaria parasites, and keyhole limpet hemocyanine. In order for an effect to be seen, antigens must be administered in suboptimal concentrations and in close temporal relationship to the IgM...
June 23, 2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28638089/a-screen-for-inhibitory-peptides-of-hepatitis-c-virus-identifies-a-novel-entry-inhibitor-targeting-e1-and-e2
#17
Peiqi Yin, Ling Zhang, Fei Ye, Yao Deng, Sha Lu, Yi-Ping Li, Leiliang Zhang, Wenjie Tan
Hepatitis C virus (HCV) entry into hepatocytes is a multistep process that represents a promising target for antiviral intervention. The viral envelope protein E1E2 plays a critical role in HCV entry. In this study, we sought to identify peptide inhibitors of HCV by screening a library of overlapping peptides covering E1E2. Screening the peptide library identified several novel anti-HCV peptides. Four peptides from glycoprotein E2 were selected for further investigation. The 50% effective dose (ED50) was approximately 5 nM for each peptide...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28602860/a-role-for-the-tetraspanin-proteins-in-salmonella-infection-of-human-macrophages
#18
Noha A Hassuna, Peter N Monk, Fawwaz Ali, Robert C Read, Lynda J Partridge
OBJECTIVE: Infected macrophages play a role in the dissemination of Salmonella and may serve as a reservoir of infection in asymptomatic carriers. However, relatively little is known about the early molecular interactions of the bacteria with these cells. We have recently shown that members of the tetraspanin family of membrane proteins are involved in the initial adhesion of a range of bacteria to host cells. This study investigated the role of tetraspanins in Salmonella enterica subsp...
June 8, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28574577/pcsk9-and-infection-a-potentially-useful-or-dangerous-association
#19
REVIEW
Farzad Kahdemi, Amir Abbas Momtazi-Borojeni, Željko Reiner, Maciej Banach, Khalid Al Rasadi, Amirhossein Sahebkar
Elevated plasma low-density lipoprotein-cholesterol (LDL-C) concentration is the most important risk factor for atherosclerotic cardiovascular diseases. Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a ubiquitously expressed serine proteinase which plays a key role in cholesterol metabolism, but has been found to be implicated in some other lipid-independent physiological processes. In this review, the role of PCSK9 was evaluated not only concerning lipid metabolism but also hepatitis C virus (HCV) infection, bacterial infections/sepsis and septic shock...
June 2, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28557183/extracellular-vesicles-do-not-contribute-to-higher-circulating-levels-of-soluble-lrp1-in-idiopathic-dilated-cardiomyopathy
#20
Santiago Roura, Carolina Gálvez-Montón, David de Gonzalo-Calvo, Ana Gámez Valero, Paloma Gastelurrutia, Elena Revuelta-López, Cristina Prat-Vidal, Carolina Soler-Botija, Aida Llucià-Valldeperas, Isaac Perea-Gil, Oriol Iborra-Egea, Francesc E Borràs, Josep Lupón, Vicenta Llorente-Cortés, Antoni Bayes-Genis
Idiopathic dilated cardiomyopathy (IDCM) is a frequent cause of heart transplantation. Potentially valuable blood markers are being sought, and low-density lipoprotein receptor-related protein 1 (LRP1) has been linked to the underlying molecular basis of the disease. This study compared circulating levels of soluble LRP1 (sLRP1) in IDCM patients and healthy controls and elucidated whether sLRP1 is exported out of the myocardium through extracellular vesicles (EVs) to gain a better understanding of the pathogenesis of the disease...
May 29, 2017: Journal of Cellular and Molecular Medicine
keyword
keyword
96257
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"