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Edta chelation therapy

Giuseppantonio Maisetta, Lucia Grassi, Semih Esin, Ilaria Serra, Mariano A Scorciapino, Andrea C Rinaldi, Giovanna Batoni
Pseudomonas aeruginosa is a major cause of chronic lung infections in cystic fibrosis (CF) patients. The ability of the bacterium to form biofilms and the presence of a thick and stagnant mucus in the airways of CF patients largely contribute to antibiotic therapy failure and demand for new antimicrobial agents able to act in the CF environment. The present study investigated the anti-P. aeruginosa activity of lin-SB056-1, a recently described semi-synthetic antimicrobial peptide, used alone and in combination with the cation chelator ethylenediaminetetraacetic acid (EDTA)...
September 16, 2017: International Journal of Molecular Sciences
Donald J Messner, Christine Surrago, Celia Fiordalisi, Wing Yin Chung, Kris V Kowdley
Iron overload disorders may be treated by chelation therapy. This study describes a novel method for isolating iron chelators from complex mixtures including plant extracts. We demonstrate the one-step isolation of curcuminoids from turmeric, the medicinal food spice derived from Curcuma longa. The method uses iron-nitrilotriacetic acid (NTA)-agarose, to which curcumin binds rapidly, specifically, and reversibly. Curcumin, demethoxycurcumin, and bisdemethoxycurcumin each bound iron-NTA-agarose with comparable affinities and a stoichiometry near 1...
August 11, 2017: Biometals: An International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Sherin Jose Chockattu, B S Deepak, K Mallikarjun Goud
CONTEXT: Being integral to root canal therapy, obturation can be performed adequately only after the removal of intracanal medicament. One technique involves the use of chelating agents such as ethylenediaminetetraacetic acid (EDTA) and citric acid. Etidronic acid, a relatively new chelator, has smear layer removal ability and lesser dentinal erosion. It is untested in calcium hydroxide (Ca[OH]2) medicament removal. AIM: The aim of this study was to compare the efficiency of irrigation protocols (EDTA, citric acid, and etidronate) in Ca(OH)2 removal...
January 2017: Journal of Conservative Dentistry: JCD
Sofia Marques, Sofia Santos, Kimberly Fremin, Agnes B Fogo
Hyperoxaluria can result in oxalate nephropathy with intratubular calcium oxalate crystallization and acute tubular injury. Primary inherited enzymatic deficiency or secondary causes such as excessive dietary intake, enteric increased absorption, or high doses of vitamin C, which is metabolized to oxalate, may underlie hyperoxaluria and oxalate nephropathy. We report a case of acute kidney injury due to oxalate nephropathy in a patient using chelating therapy with oral ethylenediamine tetra acetic acid (EDTA), intravenous supplementation with vitamin C, and chronic diarrhea and discuss the potential kidney damage these factors can cause in particular settings...
July 21, 2017: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
Zhidan Xia, Jiayu Wei, Yingniang Li, Jia Wang, Wenwen Li, Kai Wang, Xiaoli Hong, Lu Zhao, Caiyong Chen, Junxia Min, Fudi Wang
Recent studies found that mutations in the human SLC30A10 gene, which encodes a manganese (Mn) efflux transporter, are associated with hypermanganesemia with dystonia, polycythemia, and cirrhosis (HMDPC). However, the relationship between Mn metabolism and HMDPC is poorly understood, and no specific treatments are available for this disorder. Here, we generated two zebrafish slc30a10 mutant lines using the CRISPR/Cas9 system. Compared to wild-type animals, mutant adult animals developed significantly higher systemic Mn levels, and Mn accumulated in the brain and liver of mutant embryos in response to exogenous Mn...
July 2017: PLoS Genetics
Sulaiman Sultan, Shishir Murarka, Ahad Jahangir, Farouk Mookadam, A Jamil Tajik, Arshad Jahangir
The off-label use of chelation therapy (disodium edetate or EDTA) for prevention of cardiovascular disease (CVD) is widespread, despite the lack of convincing evidence for efficacy or approval from the Food and Drug Administration. After the publication of results from the National Institute of Health-sponsored Trial to Assess Chelation Therapy (TACT), a randomized controlled trial (RCT) in patients after myocardial infarction (MI), there is a renewed interest in clarifying the role of this treatment modality for patients with coronary artery disease...
August 2017: Expert Review of Clinical Pharmacology
Muhamad F Bin Othman, Nabil R Mitry, Valerie J Lewington, Philip J Blower, Samantha Y A Terry
INTRODUCTION: Despite its desirable half-life and low energy Auger electrons that travel further than for other radionuclides, (67)Ga has been neglected as a therapeutic radionuclide. Here, (67)Ga is compared with Auger electron emitter (111)In as a potential therapeutic radionuclide. METHODS: Plasmid pBR322 studies allowed direct comparison between (67)Ga and (111)In (1MBq) in causing DNA damage, including the effect of chelators (EDTA and DTPA) and the effects of a free radical scavenger (DMSO)...
March 2017: Nuclear Medicine and Biology
Maria Elena Ferrero
Exposure to environmental and occupational toxicants is responsible for adverse effects on human health. Chelation therapy is the only procedure able to remove toxic metals from human organs and tissue, aiming to treat damage related to acute and/or chronic intoxication. The present review focuses on the most recent evidence of the successful use of the chelating agent ethylenediaminetetraacetic acid (EDTA). Assessment of toxic-metal presence in humans, as well as the rationale of EDTA therapy in cardiovascular and neurodegenerative diseases, is reported...
2016: BioMed Research International
Nasim Nosoudi, Aniqa Chowdhury, Steven Siclari, Saketh Karamched, Vaideesh Parasaram, Joe Parrish, Patrick Gerard, Narendra Vyavahare
Degeneration of elastic lamina and vascular calcification are common features of vascular pathology such as aortic aneurysms. We tested whether dual therapy with targeted nanoparticles (NPs) can remove mineral deposits (by delivery of a chelating agent, ethylene diamine tetraacetic acid (EDTA)) and restore elastic lamina (by delivery of a polyphenol, pentagalloyl glucose (PGG)) to reverse moderate aneurysm development. EDTA followed by PGG NP delivery led to reduction in macrophage recruitment, matrix metalloproteinase (MMP) activity, elastin degradation and calcification in the aorta as compared to delivery of control blank NPs...
2016: Theranostics
Elizabeth Quaal Hines, Irfana Soomro, Mary Ann Howland, Robert S Hoffman, Silas W Smith
CONTEXT: Manganese-associated parkinsonism is well described in occupational settings, in chronic methcathinone users, and in patients receiving long-term total parenteral nutrition. We present a unique case of acute intravenous manganese poisoning with a systematic evaluation of hemodialysis efficacy. CASE DETAILS: A 52-year-old woman was inadvertently administered a single intravenous dose of 800 mg compounded manganese chloride at an outpatient chelation center...
July 2016: Clinical Toxicology
Andrea Giampreti, Davide Lonati, Benedetta Ragghianti, Anna Ronchi, Valeria Margherita Petrolini, Sarah Vecchio, Carlo Alessandro Locatelli
Systemic toxicity associated with cobalt (Co) and chromium (Cr) containing metal hip alloy may result in neuropathy, cardiomyopathy, and hypothyroidism. However clinical management concerning chelating therapy is still debated in literature. Here are described two metal-on-metal hip-implanted patients in which N-acetyl-cysteine decreased elevated blood metal levels. A 67-year-old male who underwent Co/Cr hip implant in September 2009 referred to our Poison Control Centre for persisting elevated Co/Cr blood levels (from March 2012 to November 2014)...
2016: Case Reports in Orthopedics
Hassan Vossoughinia, Ali Pourakbar, Samaneh Esfandiari, Masoud Sharifianrazavi
A 19-year-old woman was referred to the Emergency Surgery Department with severe abdominal pain, icterus, and anemia. The patient's clinical and paraclinical findings in addition to her occupational and social history, convinced us to assay blood lead level (BLL), which was 41/5 μg/dL. Therefore toxicology consult was performed to treat lead toxicity. Recheck of the BLL showed the level as 53/7 μg/dL. So oral chelator with succimer was started. Despite consumption of oral chelator, there was no response and the pain continued...
January 2016: Middle East Journal of Digestive Diseases
Awais Ibad, Rabia Khalid, Paul D Thompson
OBJECTIVE: We examined the effect of chelation therapy on cardiovascular diseases (CVDs). BACKGROUND: EDTA is a chelating agent that binds to metals including calcium and facilitates their excretion. Chelation with EDTA is recommended by some practitioners to treat CVD with the hypothesis that reducing calcium reduces atherosclerotic calcification of arteries. However, chelation therapy has not been approved by the Food and Drug Administration, and its effectiveness is unclear...
January 2016: Journal of Clinical Lipidology
Jeong S Kim, Sun W Jang, Miwon Son, Byoung M Kim, Myung J Kang
The oral bioavailability (BA) of risedronate sodium (RS), an antiresorptive agent, is less than 1% due to its low membrane permeability as well as the formation of non-absorbable complexes with multivalent cations such as calcium ion (Ca(2+)) in the gastrointestinal tract. In the present study, to increase oral BA of the bisphosphonate, a novel enteric-coated tablet (ECT) dosage form of RS in combination with phytic acid (IP6), a natural chelating agent recognized as safe, was formulated. The chelating behavior of IP6 against Ca(2+), including a stability constant for complex formulation was characterized using the continuous variation method...
January 20, 2016: European Journal of Pharmaceutical Sciences
Pamela Ouyang, Sheldon H Gottlieb, Valerie L Culotta, Ana Navas-Acien
The Trial to Assess Chelation Therapy (TACT) was a randomized double-blind placebo-controlled trial enrolling patients age ≥50 years with prior myocardial infarction. TACT used a 2 × 2 factorial design to study ethylene diamine tetraacetic acid (EDTA) chelation and high-dose vitamin supplementation. Chelation provided a modest but significant reduction in cardiovascular endpoints. The benefit was stronger and significant among participants with diabetes but absent in those without diabetes. Mechanisms by which chelation might reduce cardiovascular risk in persons with diabetes include the effects of EDTA chelation on transition and toxic metals...
November 2015: Current Cardiology Reports
Kelly L Scholes, Ammon T Ence, Frederick G Strathmann, Jonathan R Genzen
BACKGROUND: Rapid test strips for ethylenediaminetetraacetic acid (EDTA) can be used to verify correct specimen types for clinical assays which require, or cannot be performed on, plasma collection tubes containing EDTA anticoagulant. As the test strip reaction chemistry is based on a color change induced by chelation of bismuth from a xylenol orange complex, we hypothesized that any agent capable of chelating bismuth might induce false positive test strip reactivity. The present study was therefore designed to evaluate the potential for test strip interference by chelating agents commonly used in the treatment of trace and heavy metal toxicity...
2015: Clinical Laboratory
Ching-Yun Chen, Cherng-Jyh Ke, Ko-Chung Yen, Hui-Chen Hsieh, Jui-Sheng Sun, Feng-Huei Lin
Age-related orthopedic disorders and bone defects have become a critical public health issue, and cell-based therapy is potentially a novel solution for issues surrounding bone tissue engineering and regenerative medicine. Long-term cultures of primary bone cells exhibit phenotypic and functional degeneration; therefore, culturing cells or tissues suitable for clinical use remain a challenge. A platform consisting of human osteoblasts (hOBs), calcium-alginate (Ca-Alginate) scaffolds, and a self-made bioreactor system was established for autologous transplantation of human osteoblast cell clusters...
2015: Theranostics
Duygu Çimen, Ilgım Göktürk, Fatma Yılmaz
Iron chelation therapy can be used for the selective removal of Fe(3+) ions from spiked human plasma by ion imprinting. N-Methacryloyl-(L)-glutamic acid (MAGA) was chosen as the chelating monomer. In the first step, MAGA was complexed with the Fe(3+) ions to prepare the precomplex, and then the ion-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-glutamic acid) [PHEMAGA-Fe(3+)] cryogel column was prepared by cryo-polymerization under a semi-frozen temperature of - 12°C for 24 h. Subsequently, the template, of Fe(3+) ions was removed from the matrix by using 0...
June 2016: Artificial Cells, Nanomedicine, and Biotechnology
David Lebeaux, Véronique Leflon-Guibout, Jean-Marc Ghigo, Christophe Beloin
OBJECTIVES: Treatment of catheter-related bloodstream infections (CRBSI) is hampered by the characteristic tolerance of bacterial biofilms towards antibiotics. Our objective was to study the effect of the combination of antibiotics and the alkaline amino acid l-arginine or the cation chelator EDTA on the bacterial killing of in vitro biofilms formed by an array of clinical strains responsible for CRBSI and representative of epidemiologically relevant bacterial species. METHODS: Among 32 strains described in a previous clinical study, we focused on the most antibiotic-tolerant strains including CoNS (n = 4), Staphylococcus aureus (n = 4), Enterococcus faecalis (n = 2), Pseudomonas aeruginosa (n = 4) and Enterobacteriaceae (n = 4)...
2015: Journal of Antimicrobial Chemotherapy
Guangyu Wang
Curcumin potentiates cystic fibrosis transmembrane conductance regulator (CFTR) activation in an ATP-independent but phosphorylation-dependent manner. The underlying molecular mechanisms are unclear. Here, HEK-293T cells cultured in an Fe(3+)-containing medium were transiently transfected with CFTR constructs, and the role of the inhibitory Fe(3+) bridge between intracellular loop 3 and the regulatory domain of CFTR in this pathway was investigated. The results showed that ethylenediaminetetraacetic acid (EDTA) stimulated phosphorylation-dependent CFTR activation and the stimulation was suppressed by the deletion of the regulatory domain or the insertion of a C832A mutation that removes the Fe(3+)-binding interface...
February 24, 2015: Biochemistry
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