keyword
MENU ▼
Read by QxMD icon Read
search

Superoxide AND Tempol

keyword
https://www.readbyqxmd.com/read/29751093/thyroid-hormones-affect-nitrergic-innervation-function-in-rat-mesenteric-artery-role-of-the-pi3k-akt-pathway
#1
Pablo Llévenes, Gloria Balfagón, Javier Blanco-Rivero
We aimed to determine the influence of nitrergic innervation function on the decreased mesenteric arterial tone induced by high levels of triiodothyronine (T3), as a model of acute thyroiditis, as well as the mechanism/s implicated. We analysed in mesenteric segments from male Wistar rats the effect of 10 nmol/L T3 (2 h) on the vasomotor response to electrical field stimulation (EFS) in the presence/absence of specific neuronal NOS (nNOS) inhibitor L-NPA, or superoxide anion scavenger tempol. Nitric oxide (NO) release was measured in the presence/absence of tempol or PI3K inhibitor LY294002...
May 8, 2018: Vascular Pharmacology
https://www.readbyqxmd.com/read/29695191/high-glucose-inhibits-neural-stem-cell-differentiation-through-oxidative-stress-and-endoplasmic-reticulum-stress
#2
Xi Chen, Wei-Bin Shen, Penghua Yang, Daoyin Dong, Winny Sun, Peixin Yang
Maternal diabetes induces neural tube defects by suppressing neurogenesis in the developing neuroepithelium. Our recent study further revealed that high glucose inhibited embryonic stem cell differentiation into neural lineage cells. However, the mechanism whereby high glucose suppresses neural differentiation is unclear. To investigate whether high glucose-induced oxidative stress and endoplasmic reticulum stress (ER stress) lead to the inhibition of neural differentiation, the effect of high glucose on neural stem cell (the C17...
April 26, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29627571/arginase-overexpression-and-nadph-oxidase-stimulation-underlie-impaired-vasodilation-induced-by-advanced-glycation-end-products
#3
Hany M El-Bassossy, Thikryat Neamatallah, Khadijah S Balamash, Amani T Abushareb, Malcolm L Watson
BACKGROUND: Advanced glycation endproducts (AGEs) play a major role in the development of many vascular complications that are mediated by endothelial dysfunction. The present work aimed to investigate the mechanism by which AGEs impair vasodilation. METHODS: The effect of AGEs on vasodilation induced by acetylcholine or D NONOate was examined by incubating isolated rat aortae with different AGEs concentrations. ACh-induced nitric oxide generation was assessed using the fluorescent probe diaminofluorecein (DAF-FM)...
April 5, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29615926/circadian-differences-in-the-contribution-of-the-brain-renin-angiotensin-system-in-genetically-hypertensive-mice
#4
Kristy L Jackson, Francine Z Marques, Kyungjoon Lim, Pamela J Davern, Geoffrey A Head
Objective: Genetically hypertensive BPH/2J mice are recognized as a neurogenic model of hypertension, primarily based on sympathetic overactivity and greater neuronal activity in cardiovascular regulatory brain regions. Greater activity of the central renin angiotensin system (RAS) and reactive oxygen species (ROS) reportedly contribute to other models of hypertension. Importantly the peripheral RAS contributes to the hypertension in BPH/2J mice, predominantly during the dark period of the 24 h light cycle...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29614490/in-utero-exposure-to-fine-particulate-matter-causes-hypertension-due-to-impaired-renal-dopamine-d1-receptor-in-offspring
#5
Zhengmeng Ye, Xi Lu, Yi Deng, Xinquan Wang, Shuo Zheng, Hongmei Ren, Miao Zhang, Tingting Chen, Pedro A Jose, Jian Yang, Chunyu Zeng
BACKGROUND/AIMS: Adverse environment in utero can modulate adult phenotypes including blood pressure. Fine particulate matter (PM2.5) exposure in utero causes hypertension in the offspring, but the exact mechanisms are not clear. Renal dopamine D1 receptor (D1R), regulated by G protein-coupled receptor kinase type 4 (GRK4), plays an important role in the regulation of renal sodium transport and blood pressure. In this present study, we determined if renal D1R dysfunction is involved in PM2...
March 21, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29604595/ameliorating-effects-of-tempol-on-methotrexate-induced-liver-injury-in-rats
#6
Neslihan Pınar, Mahir Kaplan, Tümay Özgür, Oğuzhan Özcan
Methotrexate (MTX) is used in the treatment of certain types of cancers and chronic inflammatory illnesses, although the clinical use of MTX is limited due to its adverse effects, the most common of which are hepatotoxicity and nephrotoxicity. In the present study, we demonstrate the protecting influence of tempol related to oxidative stress in MTX-induced liver toxicity in rats using histopathological and biochemical parameters. The rats were divided into four groups: control group (group 1), tempol group (group 2), MTX group (group 3) and MTX + tempol group (group 4)...
March 28, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29574160/reactive-oxygen-species-participate-in-liver-function-recovery-during-compensatory-growth-in-zebrafish-danio-rerio
#7
Jirong Jia, Yazhou Zhang, Xi Yuan, Jingkai Qin, Guokun Yang, Xiaozheng Yu, Bin Wang, Caiyun Sun, Wensheng Li
Compensatory growth (CG) is defined as a phase of accelerated growth when the disadvantageous environment is improved, accompanied by metabolic adjustment. Here, we report that hepatic oxidative phosphorylation (OXPHOS) activity was enhanced during compensatory growth in zebrafish. Mitochondrial metabolism enabled the generation of reactive oxygen species (ROS), which activated the nrf2 (nuclear factor-erythroid 2-related factor 2) signaling pathway, as well as the mTOR signaling pathway. Tempol (a superoxide dismutase mimetic) treatment blocked ROS signaling in the liver as well as CG in zebrafish...
March 23, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29507270/antioxidative-effects-of-tempol-on-mitochondrial-dysfunction-in-diabetic-nephropathy
#8
Akram Ranjbar, Nejat Kheiripour, Hassan Ghasemi, Mohamad Ali Seif Rabiei, Farahnaz Dadras, Farhad Khoshjou
INTRODUCTION: Oxidative stress has a well-known role in diabetic nephropathy, and mitochondria are the major source of reactive oxygen species production. This study aimed to assess the effect of tempol, a superoxide dismutase mimetic agent, on mitochondrial antioxidant enzymes and cell viability in diabetic nephropathy. MATERIALS AND METHODS: Adult male Wistar rats were divided into 4 groups of 7 animals. Diabetes mellitus was induced by injection of streptozotocin in 2 groups, the rat in one of which were also treated with tempol for 4 weeks...
March 2018: Iranian Journal of Kidney Diseases
https://www.readbyqxmd.com/read/29458041/role-of-superoxide-ion-formation-in-hypothermia-rewarming-induced-contractile-dysfunction-in-cardiomyocytes
#9
Niccole Schaible, Young Soo Han, Torkjel Tveita, Gary C Sieck
Rewarming following accidental hypothermia is associated with circulatory collapse due primarily to impaired cardiac contractile (systolic) function. Previously, we found that reduced myofilament Ca2+ sensitivity underlies hypothermia/rewarming (H/R)-induced cardiac contractile dysfunction. This reduced Ca2+ sensitivity is associated with troponin I (cTnI) phosphorylation. We hypothesize that H/R induces reactive oxygen species (ROS) formation in cardiomyocytes, which leads to cTnI phosphorylation and reduced myofilament Ca2+ sensitivity...
April 2018: Cryobiology
https://www.readbyqxmd.com/read/29456512/local-injections-of-superoxide-dismutase-attenuate-the-exercise-pressor-reflex-in-rats-with-femoral-artery-occlusion
#10
Jihong Xing, Jian Lu, Jiahao Liu, Jianhua Li
The exercise pressor reflex is amplified in patients with peripheral artery disease (PAD) and in an experimental PAD model of rats induced by femoral artery occlusion. Heightened blood pressure worsens the restricted blood flow directed to the limbs in this disease. The purpose of this study was to determine the role played by muscle oxidative stress in regulating the augmented pressor response to static exercise in PAD. We hypothesized that limb ischemia impairs muscle superoxide dismutase (SOD) thereby leading to abnormal autonomic responsiveness observed in PAD animals, and a chronic compensation of SOD for anti-oxidation improves the exaggerated exercise pressor reflex...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29359965/tempol-a-superoxide-dismutase-mimetic-drug-prevents-chronic-ischemic-renal-injury-in-two-kidney-one-clip-hypertensive-rats
#11
Douglas Vq Nunes, Cristiane A Costa, Graziele F De Bem, Viviane Sc Cordeiro, Izabelle B Santos, Lenize Crm Carvalho, Alessandro K Jordão, Anna C Cunha, Vitor F Ferreira, Roberto S Moura, Angela C Resende, Dayane T Ognibene
Tempol, a superoxide dismutase-mimetic drug, has been shown to attenuate radical-induced damage, exerting beneficial effects in the animal models of oxidative stress and hypertension. This study evaluated the effect of Tempol on renal structural and functional alterations in two-Kidney, one-Clip hypertensive rats. In this study, young male Wistar rats had the left kidney clipped (2K1C), and sham-operated animals (Sham) were used as controls. Animals received Tempol (1mmol/L in drinking water) or vehicle for 5 weeks...
January 23, 2018: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/29351453/superoxide-inhibition-restores-endothelium-dependent-dilatation-in-aging-arteries-by-enhancing-impaired-adherens-junctions
#12
Fumin Chang, Sheila Flavahan, Nicholas A Flavahan
Endothelium-dependent, nitric oxide-mediated dilatation is impaired in aging arteries. The dysfunction reflects increased production of reactive oxygen species (ROS), is reversed by inhibiting superoxide with superoxide dismutase (SOD) mimics, and is assumed to reflect superoxide-mediated inactivation of nitric oxide. However, the dysfunction also reflects Src-dependent degradation and loss of vascular-endothelial (VE)-cadherin from adherens junctions, resulting in a selective impairment in the ability of the junctions to amplify endothelial dilatation...
April 1, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29327381/oxidative-stress-impairs-myocyte-autophagy-resulting-in-myocyte-hypertrophy
#13
Jia-Pu Wang, Rui-Fang Chi, Ke Wang, Teng Ma, Xiao-Fei Guo, Xiao-Li Zhang, Bao Li, Fu-Zhong Qin, Xue-Bin Han, Bian-Ai Fan
NEW FINDINGS: What is the central question of this study? Does oxidative stress induce impairment of autophagy that results in myocyte hypertrophy early after pressure overload? What is the main finding and its importance? In cultured myocytes, hydrogen peroxide decreased autophagy and increased hypertrophy, and inhibition of autophagy enhanced myocyte hypertrophy. In rats with early myocardial hypertrophy after pressure overload, myocyte autophagy was progressively decreased. The antioxidant N-acetyl-cysteine or the superoxide dismutase mimic tempol prevented the decrease of myocyte autophagy and attenuated myocyte hypertrophy early after pressure overload...
April 1, 2018: Experimental Physiology
https://www.readbyqxmd.com/read/29296095/free-radical-scavenging-reverses-fructose-induced-salt-sensitive-hypertension
#14
Zachary P Zenner, Kevin L Gordish, William H Beierwaltes
We have previously reported that a moderate dietary supplementation of 20% fructose but not glucose leads to a salt-sensitive hypertension related to increased proximal sodium-hydrogen exchanger activity and increased renal sodium retention. We also found that while high salt increased renal nitric oxide formation, this was retarded in the presence of fructose intake. We hypothesized that at least part of the pathway leading to fructose-induced salt-sensitive hypertension could be due to fructose-induced formation of reactive oxygen species and inappropriate stimulation of renin secretion, all of which would contribute to an increase in blood pressure...
2018: Integrated Blood Pressure Control
https://www.readbyqxmd.com/read/29229746/inhibition-of-nadph-oxidase-dependent-oxidative-stress-in-the-rostral-ventrolateral-medulla-mediates-the-antihypertensive-effects-of-acupuncture-in-spontaneously-hypertensive-rats
#15
Xue-Rui Wang, Jing-Wen Yang, Cai-Shuo Ji, Xiang-Hong Zeng, Guang-Xia Shi, Marc Fisher, Cun-Zhi Liu
Oxidative stress in the rostral ventrolateral medulla (RVLM), where the sympathetic nervous control center is located, contributes to neural mechanisms of hypertension. Acupuncture was previously reported to favorably affect high blood pressure. However, little is known about the effect of acupuncture on oxidative stress-modulated mechanisms in hypertension. This study was designed to evaluate the hypothesis that acupuncture exerts an antihypertensive effect via ameliorating oxidative stress and the redox-sensitive pathway in the RVLM of spontaneously hypertensive rats...
February 2018: Hypertension
https://www.readbyqxmd.com/read/29210997/tempol-supplementation-restores-diaphragm-force-and-metabolic-enzyme-activities-in-mdx-mice
#16
David P Burns, Izza Ali, Clement Rieux, James Healy, Greg Jasionek, Ken D O'Halloran
Duchenne muscular dystrophy (DMD) is characterized by striated muscle weakness, cardiomyopathy, and respiratory failure. Since oxidative stress is recognized as a secondary pathology in DMD, the efficacy of antioxidant intervention, using the superoxide scavenger tempol, was examined on functional and biochemical status of dystrophin-deficient diaphragm muscle. Diaphragm muscle function was assessed, ex vivo, in adult male wild-type and dystrophin-deficient mdx mice, with and without a 14-day antioxidant intervention...
December 6, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29207011/astragaloside-iv-prevents-kidney-injury-caused-by-iatrogenic-hyperinsulinemia-in-a-streptozotocin%C3%A2-induced-diabetic-rat-model
#17
Ke-Qiang He, Wei-Zu Li, Xiao-Qing Chai, Yan-Yan Yin, Yan Jiang, Wei-Ping Li
Diabetic patients are able to manage their blood glucose with exogenous insulin but, ultimately, remain at risk of diabetic nephropathy (DN). Long‑term use of insulin may lead to iatrogenic hyperinsulinemia, which has been suggested to cause kidney injury. However, there are no effective interventions for iatrogenic hyperinsulinemia leading to kidney damage. In the present paper, the hypothesis that astragaloside IV (AS‑IV), a novel saponin purified from Astragalus membranaceus (Fisch) Bunge, may prevent DN in iatrogenic hyperinsulinemic diabetic rats through antioxidative and anti‑inflammatory mechanisms was investigated...
February 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29196315/phosphorylated-ccaat-enhancer-binding-protein-%C3%AE-contributes-to-rat-hiv-related-neuropathic-pain-in-vitro-and-in-vivo-studies
#18
Hyun Yi, Shue Liu, Yuta Kashiwagi, Daigo Ikegami, Wan Huang, Hirotsugu Kanda, Takafumi Iida, Ching-Hang Liu, Keiya Takahashi, David A Lubarsky, Shuanglin Hao
Chronic pain is increasingly recognized as an important comorbidity of HIV-infected patients, however, the exact molecular mechanisms of HIV-related pain are still elusive. CCAAT/enhancer binding proteins (C/EBPs) are expressed in various tissues, including the CNS. C/EBPβ, one of the C/EBPs, is involved in the progression of HIV/AIDS, but the exact role of C/EBPβ and its upstream factors are not clear in HIV pain state. Here, we used a neuropathic pain model of perineural HIV envelope glycoprotein gp120 application onto the rat sciatic nerve to test the role of phosphorylated C/EBPβ (pC/EBPβ) and its upstream pathway in the spinal cord dorsal horn (SCDH)...
January 17, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29183043/benzo-e-pyrene-inhibits-endothelium-dependent-no-mediated-dilation-of-retinal-arterioles-via-superoxide-production-and-endoplasmic-reticulum-stress
#19
Takayuki Kamiya, Taiji Nagaoka, Tsuneaki Omae, Shinji Ono, Shinichi Otani, Akitoshi Yoshida
Purpose: To investigate whether benzo(e)pyrene (B(e)P), a toxicant in cigarette smoke, affects the endothelium-dependent nitric oxide (NO)-induced vasodilation of the retinal arterioles, and whether oxidative stress, distinct protein kinase signaling pathways, and endoplasmic reticulum (ER) stress are associated with the B(e)P-induced effect on the retinal arterioles. Methods: In this in vitro study, porcine retinal arterioles were isolated, cannulated, and pressurized without flow...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29180450/knockout-of-mitochondrial-voltage-dependent-anion-channel-type-3-increases-reactive-oxygen-species-ros-levels-and-alters-renal-sodium-transport
#20
Li Zou, Valerie Linck, Yu-Jia Zhai, Laura Galarza-Paez, Linda Li, Qiang Yue, Otor Al-Khalili, Hui-Fang Bao, He-Ping Ma, Tiffany L Thai, Jundong Jiao, Douglas C Eaton
It has been suggested that voltage-dependent anion channels (VDACs) control the release of superoxide from mitochondria. We have previously shown that reactive oxygen species (ROS) such as superoxide (O2 ̇̄ ) and hydrogen peroxide (H2 O2 ) stimulate epithelial sodium channels (ENaCs) in sodium-transporting epithelial tissue, including cortical collecting duct (CCD) principal cells. Therefore, we hypothesized that VDACs could regulate ENaC by modulating cytosolic ROS levels. Herein, we find that VDAC3-knockout(KO) mice can maintain normal salt and water balance on low-salt and high-salt diets...
February 2, 2018: Journal of Biological Chemistry
keyword
keyword
96192
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"